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https://www.readbyqxmd.com/read/28944999/systemic-redistribution-of-the-intramyocardially-injected-mesenchymal-stem-cells-by-repeated-remote-ischaemic-post-conditioning
#1
Qin Jiang, Tao Yu, Keli Huang, Hao Zhang, Zhe Zheng, Shengshou Hu
We investigated the effect of repeated remote ischaemic post-conditioning (RIPoC) on the organ distribution of the intramyocardially injected MSCs in rat myocardial ischemia model. Myocardial ischemia of adult female Sprague-Dawley rats was induced by 30-min. obstruction of the left anterior descending coronary artery. Repeated RIPoC was induced after ischemia with three cycles of 5-min. occlusion and reperfusion of the limb with the frequency of half a day, 1 or 2 days, respectively. Compared with that by single RIPoC, repeated RIPoC transiently reduced oxidative stress, lipid peroxidation and inflammation in ischaemic myocardium; the gene expression of stromal cell-derived factor-1 alpha (SDF-1α) was consistently induced by repeated RIPoC procedures...
September 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28944839/alteration-of-oxidative-stress-and-inflammatory-cytokines-induces-apoptosis-in-diabetic-nephropathy
#2
Jibin Sha, Bo Sui, Xiaoqing Su, Qingfang Meng, Chenggang Zhang
Diabetic nephropathy (DN) is one of the most significant long‑term complications in terms of morbidity and mortality for diabetic patients; however, the exact cause remains unknown. To address this, the DN model was established, and oxidative stress indexes, including malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH‑Px), and inflammatory cytokines, includinginterleukin‑6 (IL‑6), tumor necrosis factor‑alpha (TNF‑α) and transforming growth factor‑beta (TGF‑β), were examined by ELISA...
September 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28943392/an-integrative-analysis-of-chemically-induced-cirrhosis-associated-hepatocarcinogenesis-histological-biochemical-and-molecular-features
#3
Guilherme Ribeiro Romualdo, Tony Fernando Grassi, Renata Leme Goto, Mariana Baptista Tablas, Lucas Tadeu Bidinotto, Ana Angélica Henrique Fernandes, Bruno Cogliati, Luís Fernando Barbisan
This study aimed the integrative characterization of morphological, biochemical and molecular features of chemically-induced cirrhosis-associated hepatocarcinogenesis. Thus, male Wistar rats were submitted to a diethylnitrosamine (DEN)/thioacetamide (TAA)-induced model. Liver tissue was processed for global gene expression, histopathological and collagen evaluations; as well as immunohistochemical and oxidative stress analysis. Gene Ontology and functional analysis showed the upregulation of extracellular matrix deposition genes, such as collagen type I alpha 1 and 2 (Col1α1 and Col1α2) and tissue inhibitor of metalloproteinase 1 and 2 genes (Timp1 and Timp2)...
September 21, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28943358/custom-glycosylation-of-cells-and-proteins-using-cyclic-carbamate-derivatized-oligosaccharides
#4
Marek W J Whitehead, Nikolay Khanzhin, Lubor Borsig, Thierry Hennet
The structural complexity of glycosylation restrains the functional characterization of glycans. We present a versatile carbohydrate ligation technique based on the reaction of cyclic carbamates with primary amines. Cyclic-carbamate-derivatized carbohydrates can be added to primary amine-containing molecules in aqueous solution to yield glycoconjugates. This method enabled the presentation of carbohydrate epitopes on live animal cells, as shown by the acquisition of E-selectin binding sites on mouse MC-38 cells decorated with 3-fucosyllactose or 3-fucosyl-3-sialyllactose...
September 6, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28942850/characterization-and-complete-genome-sequences-of-l-rhamnosus-dsm-14870-and-l-gasseri-dsm-14869-contained-in-the-ecovag-%C3%A2-probiotic-vaginal-capsules
#5
Harold Marcotte, Kasper Krogh Andersen, Yin Lin, Fanglei Zuo, Zhu Zeng, Per Göran Larsson, Erik Brandsborg, Gunnar Brønstad, Lennart Hammarström
Lactobacillus rhamnosus DSM 14870 and Lactobacillus gasseri DSM 14869 were previously isolated from the vaginal epithelial cells (VEC) of healthy women and selected for the development of the vaginal EcoVag(®) probiotic capsules. EcoVag(®) was subsequently shown to provide long-term cure and reduce relapse of bacterial vaginosis (BV) as an adjunct to antibiotic therapy. To identify genes potentially involved in probiotic activity, we performed genome sequencing and characterization of the two strains. The complete genome analysis of both strains revealed the presence of genes encoding functions related to adhesion, exopolysaccharide (EPS) biosynthesis, antimicrobial activity, and CRISPR adaptive immunity but absence of antibiotic resistance genes...
December 2017: Microbiological Research
https://www.readbyqxmd.com/read/28942652/polyphenols-from-blossoms-of-citrus-aurantium-l-var-amara-engl-show-significant-anti-complement-and-anti-inflammatory-effects
#6
Chun-Yan Shen, Jian-Guo Jiang, Chun-Ling Huang, Wei Zhu, Chao-Yang Zheng
Citrus aurantium L. var. amara Engl. (CAVA) was traditionally used as a digestant or expectorant in China. Crude polyphenols (CAVAP-W) extracted from blossoms of CAVA were mainly composed of eriocitrin/neoeriocitrin, eriocitrin/neoeriocitrin, rhoifolin, hesperidin, naringin, rutin, veronicastroside, neohesperidin and hesperetin by LC-MS analysis. CAVAP-W showed significant anti-complement and anti-inflammatory effects. Due to the close relationship between anti-complement and anti-inflammatory activity, the anti-inflammatory effect was further investigated and the results showed that CAVAP-W significantly suppressed production of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and mRNA expression of inducible nitric oxide synthase (iNOS), IL-6, TNF-α, IL-1β and cyclooxygenase-2 (COX-2) in lipopolysaccharides-stimulated RAW264...
September 24, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28942049/purification-of-native-m-vogae-and-h-contortus-tubulin-by-tog-affinity-chromatography
#7
Beatriz Munguía, Ramiro Teixeira, Victoria Veroli, Elisa Melian, Jenny Saldaña, Mahia Minteguiaga, Mario Señorale, Mónica Marín, Laura Domínguez
Microtubules are non-covalent cylindrical polymers formed by alpha- and beta-tubulin heterodimer units, crucial for cell division, intracellular transport, motility and differentiation. This makes them very attractive pharmacological targets exploited to develop different drugs such as anthelmintics, antifungals, and antineoplastics. In this work, in order to establish an in vitro target-based screen to integrate to the search for new anthelmintics, we explored the extraction of native assembly-competent tubulin from two helminth parasites: Mesocestoides vogae tetrathyridia (syn...
September 20, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28941732/the-adaptor-protein-alpha-syntrophin-is-reduced-in-human-non-alcoholic-steatohepatitis-but-is-unchanged-in-hepatocellular-carcinoma
#8
Rein-Fischboeck Lisa, Pohl Rebekka, M Haberl Elisabeth, S Weiss Thomas, Buechler Christa
The adaptor protein alpha-syntrophin (SNTA) is differentially expressed in varying types of cancer and affects triglyceride levels, inflammatory response and cell proliferation. However, little is known about the expression of SNTA in liver diseases. Non-alcoholic steatohepatitis (NASH) is characterized by hepatic steatosis, inflammation and eventually fibrosis, and may progress to hepatocellular carcinoma (HCC). Here, SNTA mRNA was analyzed in liver tissues from 71 non-alcoholic fatty liver disease patients and 32 controls to assess associations with disease characteristics...
September 20, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28941240/dynamic-changes-in-brain-mesenchymal-perivascular-cells-associate-with-multiple-sclerosis-disease-duration-active-inflammation-and-demyelination
#9
Ellen Iacobaeus, Rachael V Sugars, Anton Törnqvist Andrén, Jessica J Alm, Hong Qian, Janek Frantzen, Jia Newcombe, Kanar Alkass, Henrik Druid, Matteo Bottai, Matias Röyttä, Katarina Le Blanc
Vascular changes, including blood brain barrier destabilization, are common pathological features in multiple sclerosis (MS) lesions. Blood vessels within adult organs are reported to harbor mesenchymal stromal cells (MSCs) with phenotypical and functional characteristics similar to pericytes. We performed an immunohistochemical study of MSCs/pericytes in brain tissue from MS and healthy persons. Post-mortem brain tissue from patients with early progressive MS (EPMS), late stage progressive MS (LPMS), and healthy persons were analyzed for the MSC and pericyte markers CD146, platelet-derived growth factor receptor beta (PDGFRβ), CD73, CD271, alpha-smooth muscle actin, and Ki67...
September 23, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28941035/role-of-estrogen-receptor-alpha-and-beta-in-bladder-tissue-in-patients-with-clinical-diagnosis-of-benign-prostatic-hyperplasia
#10
R Bhattar, S S Yadav, V Tomar, A Mittal, G Gangkak, J Mehta
OBJECTIVE: To study the differential expression of estrogen receptor (ER) subtypes in human urinary bladder tissue using immunohistochemistry (IHC) methods and explores their correlation with various measures of LUTS. METHODS: In this prospective case control study, case group (group A) comprised of 34 patients having bothersome LUTS underwent transurethral resection of prostate (TURP). 19 age matched otherwise normal patients who underwent cystoscopy as an investigation for microscopic hematuria were categorized in control group (group B)...
September 21, 2017: BJU International
https://www.readbyqxmd.com/read/28940415/mesenchymal-stromal-cells-ameliorate-acute-allergic-rhinitis-in-rats
#11
Chunlei Li, Yanxia Fu, Yinyin Wang, Yanhua Kong, Mengdi Li, Danhui Ma, Wanli Zhai, Hao Wang, Yuting Lin, Sihan Liu, Fangli Ren, Jun Li, Yi Wang
Mesenchymal stromal cells (MSCs) have been extensively investigated as a potential antiinflammatory treatment in many inflammatory-related diseases; however, it remains unclear whether MSCs could be used to treat acute allergic rhinitis. A rat model of allergic rhinitis was treated with MSCs. The effect of MSCs on the inflammation of allergic rhinitis was evaluated by sneezing, nose rubbing, the pathology of the nasal mucosa, and the expression of interleukin 4, tumour necrosis factor alpha, and immunoglobulin E in the serum of rats...
September 20, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28940304/the-foxa2-transcription-factor-is-frequently-somatically-mutated-in-uterine-carcinosarcomas-and-carcinomas
#12
Matthieu Le Gallo, Meghan L Rudd, Mary Ellen Urick, Nancy F Hansen, Maria J Merino, David G Mutch, Paul J Goodfellow, James C Mullikin, Daphne W Bell
BACKGROUND: Uterine carcinosarcomas (UCSs) are a rare but clinically aggressive form of cancer. They are biphasic tumors consisting of both epithelial and sarcomatous components. The majority of uterine carcinosarcomas are clonal, with the carcinomatous cells undergoing metaplasia to give rise to the sarcomatous component. The objective of the current study was to identify novel somatically mutated genes in UCSs. METHODS: We whole exome sequenced paired tumor and nontumor DNAs from 14 UCSs and orthogonally validated 464 somatic variants using Sanger sequencing...
September 21, 2017: Cancer
https://www.readbyqxmd.com/read/28939747/a-pilot-trial-of-humanized-anti-gd2-monoclonal-antibody-hu14-18k322a-with-chemotherapy-and-natural-killer-cells-in-children-with-recurrent-refractory-neuroblastoma
#13
Sara M Federico, M Beth McCarville, Barry L Shulkin, Paul M Sondel, Jacquelyn A Hank, Paul Hutson, Michael Meagher, Aaron Shafer, Catherine Y Ng, Wing Leung, William E Janssen, Jianrong Wu, Shenghua Mao, Rachel C Brennan, Victor M Santana, Alberto Pappo, Wayne L Furman
PURPOSE: Anti-GD2 monoclonal antibodies (mAb), acting via antibody-dependent cell-mediated cytotoxicity, may enhance the effects of chemotherapy. This pilot trial investigated a fixed dose of a unique anti-GD2 mAb, hu14.18K322A, combined with chemotherapy, cytokines and haploidentical natural killer (NK) cells. EXPERIMENTAL DESIGN: Children with recurrent/refractory neuroblastoma received up to six courses of hu14.18K322A (40mg/m2/dose, days 2-5), GMCSF and IL-2 with chemotherapy: cyclophosphamide/topotecan (courses 1,2), irinotecan/temozolomide (courses 3,4) and ifosfamide/carboplatin/etoposide (courses 5,6)...
September 22, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28938749/the-testicular-transcriptome-associated-with-spermatogonia-differentiation-initiated-by-gonadotrophin-stimulation-in-the-juvenile-rhesus-monkey-macaca-mulatta
#14
Suresh Ramaswamy, William H Walker, Paula Aliberti, Rahil Sethi, Gary R Marshall, Alyxzandria Smith, Seyedmehdi Nourashrafeddin, Alicia Belgorosky, Uma R Chandran, Mark P Hedger, Tony M Plant
STUDY QUESTION: What is the genetic landscape within the testis of the juvenile rhesus monkey (Macaca mulatta) that underlies the decision of undifferentiated spermatogonia to commit to a pathway of differentiation when puberty is induced prematurely by exogenous LH and FSH stimulation? SUMMARY ANSWER: Forty-eight hours of gonadotrophin stimulation of the juvenile monkey testis resulted in the appearance of differentiating B spermatogonia and the emergence of 1362 up-regulated and 225 down-regulated testicular mRNAs encoding a complex network of proteins ranging from enzymes regulating Leydig cell steroidogenesis to membrane receptors, and from juxtacrine and paracrine factors to transcriptional factors governing spermatogonial stem cell fate...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28938560/inflammatory-factor-tnf-%C3%AE-promotes-the-growth-of-breast-cancer-via-the-positive-feedback-loop-of-tnfr1-nf-%C3%AE%C2%BAb-and-or-p38-p-stat3-hbxip-tnfr1
#15
Xiaoli Cai, Can Cao, Jiong Li, Fuquan Chen, Shuqin Zhang, Bowen Liu, Weiying Zhang, Xiaodong Zhang, Lihong Ye
In the connection between inflammation and cancer development, tumor necrosis factor-alpha (TNF-α) contributes to the tumorigenesis. However, the underlying mechanism remains poorly understood. In this study, we report that TNF-α enhances the growth of breast cancer through up-regulation of oncoprotein hepatitis B X-interacting protein (HBXIP). Our data showed that the levels of TNF-α were positively related to those of HBXIP in clinical breast cancer tissues. Moreover, TNF-α could up-regulate HBXIP in breast cancer cells...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938448/novel-autosomal-dominant-pth-gene-signal-sequence-mutation-in-a-family-with-familial-isolated-hypoparathyroidism
#16
Luigia Cinque, Angelo Sparaneo, Laura Penta, Amedea Mencarelli, Daniela Rogaia, Susanna Esposito, Federico Pio Fabrizio, Filomena Baorda, Alberto Verrotti, Alberto Falorni, Gabriela Stangoni, Geoffrey N Hendy, Vito Guarnieri, Paolo Prontera
Context: Familial isolated hypoparathyroidism (FIH) is a genetically heterogeneous disorder due to mutations of the calcium-sensing receptor (CASR), glial cells missing-2 (GCM2), guanine nucleotide binding protein alpha 11 (GNA11), or parathyroid hormone (PTH) genes. Thus far, only 4 cases with homozygous and 2 cases with heterozygous mutations in the PTH gene have been reported. Objective: To clinically describe an FIH family and identify and characterize the causal gene mutation...
August 18, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28938189/the-potent-suppressive-effect-of-%C3%AE-d-mannuronic-acid-m2000-on-molecular-expression-of-the-tlr-nf-kb-signaling-pathway-in-ankylosing-spondylitis-patients
#17
Maryam Roozbehkia, Mahdi Mahmoudi, Somaye Aletaha, Nima Rezaei, Mohammad Javad Fattahi, Fahimeh Jafarnezhad-Ansariha, Anis Barati, Abbas Mirshafiey
Ankylosing spondylitis (AS) is a chronic inflammatory rheumatic disease characterized by the inflammation of sacroiliac joints and axial skeleton. A combination of genetic, environmental and immunological factors are involved in AS's pathogenesis. TLRs are type I transmembrane glycoproteins that play a crucial role in the innate immune responses against invading pathogens. Observational studies have demonstrated a possible association between TLR dysregulation and AS. The β-d-mannuronic acid (M2000), as a novel NSAID with immunosuppressive property, has shown an inhibitory effect on Toll-like receptor (TLR) 2, 4 signaling in HEK293 cells...
September 19, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28937406/tolerance-in-xenotransplantation
#18
Kazuhiko Yamada, Megan Sykes, David H Sachs
PURPOSE OF REVIEW: This review describes recent progress in tolerance-inducing strategies across xenogeneic immunological barriers as well as the potential benefit of a tolerance strategy for islets and kidney xenotransplantation. RECENT FINDINGS: Using advanced gene editing technologies, xenotransplantation from multitransgenic alpha-1,3-galactosyltransferase knockout pigs has demonstrated marked prolongation of renal xenograft survival, ranging from days to greater than several months for life-supporting kidneys, and more than 2 years in a heterotopic nonlife-supporting cardiac xenograft model...
September 20, 2017: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/28937367/associations-between-epidermal-growth-factor-receptor-and-topoisomerase-ii-alpha-gene-copy-number-variations-human-papillomavirus-positivity-and-cytologic-analysis-in-cervical-cell-lesions
#19
Işın Kaya, Oya Nermin Sivrikoz, Özdal Etlik, Abdülkadir Gök
BACKGROUND: Cervical cancer is the second most common gynecologic cancer worldwide. Human papillomavirus (HPV) infection is a leading etiological factor in cervical carcinoma.The aim of this study was to compare HPV positivity, EGFR and TOP2A gene copy number variations and cervical cytologic findings. MATERIALS AND METHODS: The study group comprised 100 female volunteers between 21-64 years old. Cytologic analysis was performed using the liquid-based cytology technique...
July 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28937296/comparison-of-e-cadherin-with-stat3-and-apoptosis-regulators-bak-and-bcl-xl-in-endometrioid-adenocarcinomas-of-different-er-alpha-immunoprofile
#20
Urszula Sulkowska, Andrzej Wincewicz, Luiza Kanczuga-Koda, Mariusz Koda, Stanislaw Sulkowski
E-cadherin is a factor of good prognosis in endometrioid adenocarcinomas, while STAT3 is an oncogenic driver of carcinogenesis. E-cadherin, Bak, Bcl-xL and STAT3 were immunohistochemically detected in 78 human endometrioid adenocarcinomas. E-cadherin correlated with STAT3 (p <. 001, r = 0.537) as well as Bak (p = .005, r = 0.314) and Bcl-xL (p = .002, r = 0.340) in the whole study group. In G2 tumors, E-cadherin associated with Bak (p = .021, r = 0.319), Bcl-xL (p = .026, r = 0...
September 22, 2017: Gynecological Endocrinology
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