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https://www.readbyqxmd.com/read/29331935/disruption-of-thyroid-hormone-sulfotransferase-activity-by-brominated-flame-retardant-chemicals-in-the-human-choriocarcinoma-placenta-cell-line-bewo
#1
Christopher P Leonetti, Craig M Butt, Heather M Stapleton
Brominated flame retardants (BFRs) have been shown to disrupt thyroid hormone (TH) homeostasis through multiple mechanisms, including inhibition of enzymes that regulate intracellular levels of THs, such as sulfotransferases (SULTs). The placenta plays a critical role in helping to maintain TH levels during fetal development and expresses SULTs. This is concerning given that disruption of TH regulation within the placenta could potentially harm the developing fetus. In this study, we investigated the effects of two polybrominated diphenyl ethers (PBDEs), two hydroxylated PBDEs, and 2,4,6-tribromophenol (2,4,6-TBP) on TH SULT activity in a choriocarcinoma placenta cell line (BeWo)...
December 28, 2017: Chemosphere
https://www.readbyqxmd.com/read/29331497/dietary-antioxidant-micronutrients-alter-mucosal-inflammatory-risk-in-a-murine-model-of-genetic-and-microbial-susceptibility
#2
Joseph F Pierre, Reinhard Hinterleitner, Romain Bouziat, Nathan A Hubert, Vanessa Leone, Jun Miyoshi, Bana Jabri, Eugene B Chang
Inflammatory bowel diseases (IBD) are caused by the convergence of microbial, environmental, and genetic factors. Diet significantly alters these interactions by affecting both the host and microbiome. Using a mucosal inflammatory model that resembles the human condition of ileal pouchitis, we investigated the effects of Control (CONT) or Antioxidant (AOX) diet, containing pharmacologically relevant levels of 4 micronutrients, on disease risk in wild-type and IL-10-/- animals following surgical self-filling (SF) ileal blind loop placement...
December 10, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29330928/specific-labelling-of-myonuclei-by-an-antibody-against-pericentriolar-material-1-pcm1-on-skeletal-muscle-tissue-sections
#3
I M Winje, M Bengtsen, E Eftestøl, I Juvkam, J C Bruusgaard, K Gundersen
AIM: Skeletal muscle is a heterogeneous tissue containing several different cell types and only about 40 - 50% of the cell nuclei within the tissue belong to myofibres. Existing technology, attempting to distinguish myonuclei from other nuclei at the light microscopy level, has led to controversies in our understanding of the basic cell biology of muscle plasticity. The present study aims at demonstrating that an antibody against the protein Pericentriolar material 1 (PCM1) can be used to reliably identify myonuclei on histological cross sections from humans, mice and rats...
January 13, 2018: Acta Physiologica
https://www.readbyqxmd.com/read/29330807/evaluation-of-macrophage-polarization-in-pancreatic-cancer-microenvironment-under-hypoxia
#4
Kuldeep S Attri, Kamiya Mehla, Pankaj K Singh
Hypoxic microenvironment found in pancreatic ductal adenocarcinoma and other solid tumors is central to physiological and metabolic alterations of immune cells that significantly impact tumor growth dynamics. Hypoxic adaptations in the immune cells are primarily mediated by the stabilization of hypoxia-inducible factor-1 alpha (HIF-1α), which regulates cellular metabolism by modulating glycolysis and other interconnected metabolic pathways. HIF-1α plays distinct roles in M1 and M2 macrophage polarization, which, in turn, regulates tumor cell immune escape and growth...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29330794/hypoxia-mediated-in-vivo-tumor-glucose-uptake-measurement-and-analysis
#5
Surendra K Shukla, Scott E Mulder, Pankaj K Singh
Most solid tumors are hypoxic in nature due to the limited supply of oxygen to internal tissues. Hypoxia plays an important role in metabolic adaptations of tumors that contribute significantly to cancer pathogenesis. Among the several metabolic alterations induced by hypoxia, hypoxia-mediated increased glucose uptake serves as the hallmark of metabolic reprogramming. Hypoxia-mediated stabilization of hypoxia-inducible factor-1 alpha (HIF-1α) transcription factor leads to altered expression of several glycolytic genes and glucose transporters, which results in increased glucose uptake by tumor cells...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29330793/hypoxia-induced-metabolomic-alterations-in-pancreatic-cancer-cells
#6
Venugopal Gunda, Sushil Kumar, Aneesha Dasgupta, Pankaj K Singh
Hypoxic conditions in the pancreatic tumor microenvironment lead to the stabilization of hypoxia-inducible factor-1 alpha (HIF-1α), which acts as the master regulator of cancer cell metabolism. HIF-1α-mediated metabolic reprogramming results in large-scale metabolite perturbations. Characterization of the metabolic intermediates and the corresponding metabolic pathways altered by HIF-1α would facilitate the identification of therapeutic targets for hypoxic microenvironments prevalent in pancreatic ductal adenocarcinoma and other solid tumors...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29330774/a-collection-of-xy-female-cell-lines
#7
Fumio Kasai, Malcolm A Ferguson-Smith
Discordance between sexual phenotype and the 46,XY sex chromosome complement may be found in certain disorders of sexual development (DSD). Many of these DSD patients with female external genitalia and secondary sex characteristics have undescended testes and male internal genitalia. Causative mutations involving genes of the sex determining pathway, including the androgen receptor, SRY and the 5-alpha-reductase genes, are well-known, but the origin of other cases remain unresolved. In this report, we introduce our collection of lymphoblastoid lines derived from female patients with a 46,XY karyotype...
January 12, 2018: Human Cell
https://www.readbyqxmd.com/read/29330517/iron-induced-calcification-in-human-aortic-vascular-smooth-muscle-cells-through-interleukin-24-il-24-with-without-tnf-alpha
#8
Sayuri Kawada, Yasuyuki Nagasawa, Mutsuki Kawabe, Hideki Ohyama, Aritoshi Kida, Nahoko Kato-Kogoe, Masayoshi Nanami, Yukiko Hasuike, Takahiro Kuragano, Hiromitsu Kishimoto, Keiji Nakasho, Takeshi Nakanishi
In CKD patients, arteriosclerotic lesions, including calcification, can occur in vascular smooth muscle cells in a process called Moenckeberg's medial arteriosclerosis. Iron overload induces several complications, including the acceleration of arteriosclerosis. However, the relationship between Moenckeberg's arteriosclerosis in vascular smooth muscle cells and iron accumulation has remained unknown. We tested the accelerated effect of iron on calcification in cultured human aortic vascular smooth muscle cells (HASMCs)...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29330471/slc26a3-dra-prevents-tnf-alpha-induced-barrier-dysfunction-and-dextran-sulfate-sodium-induced-acute-colitis
#9
Xiangming Ding, Dongxiao Li, Mengke Li, Han Wang, Qin He, Yunwu Wang, Hongbing Yu, Dean Tian, Qin Yu
SLC26A3 encodes a Cl-/HCO3- ion transporter that is also known as downregulated in adenoma (DRA) and is involved in HCO3-/mucus formation. The role of DRA in the epithelial barrier has not been previously established. In this study, we investigated the in vivo and in vitro mechanisms of DRA in the colon epithelial barrier. Immunofluorescence (IF) and co-immunoprecipitation (co-IP) studies reveal that DRA binds directly to tight junction (TJ) proteins and affects the expression of TJ proteins in polarized Caco-2BBe cells...
January 12, 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29330447/human-placental-derived-adherent-stromal-cells-co-induced-with-tnf-%C3%AE-and-ifn-%C3%AE-inhibit-triple-negative-breast-cancer-in-nude-mouse-xenograft-models
#10
Hoshea Allen, Niva Shraga-Heled, Michal Blumenfeld, Tamar Dego-Ashto, Dana Fuchs-Telem, Ariel Gilert, Zami Aberman, Racheli Ofir
Culturing 3D-expanded human placental-derived adherent stromal cells (ASCs) in the presence of tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) transiently upregulated the secretion of numerous anti-proliferative, anti-angiogenic and pro-inflammatory cytokines. In a 3D-spheroid screening assay, conditioned medium from these induced-ASCs inhibited proliferation of cancer cell lines, including triple-negative breast cancer (TNBC) lines. In vitro co-culture studies of induced-ASCs with MDA-MB-231 human breast carcinoma cells, a model representing TNBC, supports a mechanism involving immunomodulation and angiogenesis inhibition...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29330187/acquisition-of-a-novel-sulfur-oxidizing-symbiont-in-the-gutless-marine-worm-inanidrilus-exumae
#11
C Bergin, C Wentrup, N Brewig, A Blazejak, C Erséus, O Giere, M Schmid, P De Wit, N Dubilier
Gutless phallodrilines are marine annelid worms without a mouth or gut, which live in an obligate association with multiple bacterial endosymbionts that supply them with nutrition. In this study, we discovered an unusual symbiont community in the gutless phallodriline Inanidrilus exumae that differs markedly from the microbiome of all other 22 examined host species. Comparative 16S rRNA gene sequence analysis and fluorescence in situ hybridization revealed that I. exumae harboured co-occurring gamma-, alpha- and deltaproteobacterial symbionts, while all other known host species harbour gamma- and either alpha- or deltaproteobacterial symbionts...
January 12, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29330116/tnf-%C3%AE-increases-staphylococcus-aureus-induced-death-of-human-alveolar-epithelial-cell-line-a549-associated-with-rip3-mediated-necroptosis
#12
Shun-Hang Wen, Luo-Na Lin, Hu-Jun Wu, Lu Yu, Li Lin, Li-Li Zhu, Hai-Yan Li, Hai-Lin Zhang, Chang-Chong Li
AIM: To explore the role of tumor necrosis factor-alpha (TNF-α) on Staphylococcus aureus-induced necroptosis in alveolar epithelial cells. MAIN METHODS: The A549 alveolar epithelial cell line was pretreated with small interfering RNA (siRNA) against receptor interacting protein-3 (RIP3) and then stimulated by S. aureus, where some cells were pretreated with TNF-α or TNF-α with anti-TNF-α antibody simultaneously. A549 cell death was assessed using lactate dehydrogenase (LDH) leakage and flow cytometry analyses...
January 9, 2018: Life Sciences
https://www.readbyqxmd.com/read/29330050/production-and-characterization-of-a-novel-site-specific-modifiable-anti-ox40-receptor-single-chain-variable-fragment-for-targeted-drug-delivery
#13
Aki Tanabe, Kazumi Nakano, Makoto Nakakido, Satoru Nagatoishi, Yuetsu Tanaka, Kouhei Tsumoto, Kaoru Uchimaru, Toshiki Watanabe
OX40 receptor (tumor necrosis factor receptor superfamily, member 4; CD134) is a T-cell co-stimulatory molecule that plays an important role in T-cell activation and survival. OX40 receptor is activated by its ligand, OX40L; and modulation of the OX40-OX40L interaction is a promising target for the treatment of autoimmune diseases and cancers. Here, we generated a high-affinity anti-OX40 single-chain variable fragment carrying a C-terminal cysteine residue (scFvC). Physicochemical and functional analyses revealed that the scFvC bound to OX40-expressing cells and was internalized via OX40-mediated endocytosis without inducing phosphorylation of IκBα (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), an important complex in the classical NFκB (nuclear factor kappa-light-chain-enhancer of activated B cells) signaling pathway...
January 9, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29329680/correlation-of-severity-of-chronic-obstructive-pulmonary-disease-with-potential-biomarkers
#14
Seema Singh, S K Verma, Santosh Kumar, M K Ahmad, Anuradha Nischal, S K Singh, R K Dixit
BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a non-specific inflammation, which involves the airways, lung parenchyma and pulmonary vessels. The inflammation causes the activation of inflammatory cells and the release of various inflammatory mediators such as interleukin-1 beta, interleukin-6 and tumor necrosis factor alpha (TNF-a). The present study was designed to assess the serum cytokines [Interleukin-1β (IL-1β), Interleukin-6 (IL-6), Tumor necrosis factor-α (TNF-α)] levels in chronic obstructive pulmonary disease (COPD) patients and they were correlated with severity of disease by spirometric measurements...
January 9, 2018: Immunology Letters
https://www.readbyqxmd.com/read/29329619/hepatic-fibropoiesis-in-dogs-naturally-infected-with-leishmania-leishmania-infantum-treated-with-liposome-encapsulated-meglumine-antimoniate-and-allopurinol
#15
R S Castro, I F G de Amorim, R A Pereira, S M Silva, L J Pinheiro, A J W Pinto, E G Azevedo, C Demicheli, M M V Caliari, D M Mosser, M S M Michalick, Frédéric J G Frezard, Wagner L Tafuri
Hepatic fibropoiesis in canine visceral leishmaniasis (CVL) were evaluated by histological (morphometrical collagen deposition) and immunohistochemical assays characterizing alpha-actin (α-SMA), vimentin, calprotectin (L1 antigen), and TGF-β in 46 naturally infected dogs with Leishmania infantum treated with liposome-encapsulated meglumine antimoniate and allopurinol separately and in combination. Six treatment groups were defined: meglumine antimoniate encapsulated in nanometric liposomes (LMA), allopurinol (ALLOP); liposome-encapsulated meglumine antomoniate combined with allopurinol (LMA+ALLOP); empty liposomes (LEMP); empty liposomes combined with allopurinol (LEMP+ALLOP) and saline...
January 30, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29329251/pharmacological-investigation-of-the-anti-inflammation-and-anti-oxidation-activities-of-diallyl-disulfide-in-a-rat-emphysema-model-induced-by-cigarette-smoke-extract
#16
Yan Liu, Ang Li, Xiuli Feng, Xiao Sun, Xiaosong Zhu, Zhongxi Zhao
Diallyl disulfide (DADS) is the main organosulfur ingredient in garlic, with known antioxidant and anti-inflammatory activities. The aim of the present study was to investigate the effect of DADS on reducing the inflammation and redox imbalance in a rat emphysema model that was induced by intraperitoneal injection of cigarette smoke extract (CSE). Briefly, DADS exerted an anti-inflammation effect on emphysema rats through decreasing cell influx in the bronchoalveolar lavage fluid (BALF) and suppressing pro-inflammation cytokine production including tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) via inhibiting the NF-κB pathway...
January 12, 2018: Nutrients
https://www.readbyqxmd.com/read/29329129/primary-cutaneous-spindle-cell-squamous-carcinoma-expressing-smooth-muscle-actin-diagnostic-pitfalls
#17
Abdullahi Ahmad, Hye Jin Chung, Jag Bhawan
Alpha smooth muscle actin (SMA) belongs to the actin proteins. It is a known immunohistochemical marker for tumors of mesenchymal origin. There have been reports of expression of SMA in certain epithelial malignancies in the head and neck and genital regions. In this study, the authors report a primary cutaneous spindle cell squamous carcinoma expressing SMA. Both high- and low-molecular-weight keratins and p63 were positive, and S100 protein, SOX10, MART-1/Melan-A, and muscle-specific actin stains were negative...
January 11, 2018: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/29329019/salidroside-influences-the-cellular-cross-talk-of-human-fetal-lung-diploid-fibroblasts-a-proteomic-approach
#18
Wenmin Xing, Wenyan Gao, Huili Su, Sanying Wang, Jing Zhang, Genxiang Mao, Jing Yan
Senescence is a complex multiple factor proces, which is still poorly understood. The purpose of this study was to find the proteome of cultured human fetal lung diploid fibroblasts (2BS) of different population doubling (PD), as well as the altered proteome induced by salidroside (SAL) in 2BS cells. Proteins were identified by two-dimensional electrophoresis (2-DE) combining matrix-assisted laser desorption/ionization-time and flight mass spectrometry (MAL DI-TOF/MS). As a result, we found 16 proteins with two-fold variations in senescent cells or after SAL treatment, some being reduced such as reticulocalbin-1, heat shock protein beta-6, elongation factor 1-delta, F-actin-capping protein subunit alpha-1, and chloride intracellular channel 1...
January 5, 2018: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/29328990/the-amyloidogenicity-of-the-influenza-virus-pb1-derived-peptide-sheds-light-on-its-antiviral-activity
#19
Yana A Zabrodskaya, Dmitry V Lebedev, Marja A Egorova, Aram A Shaldzhyan, Alexey V Shvetsov, Alexander I Kuklin, Daria S Vinogradova, Nikolay V Klopov, Oleg V Matusevich, Taisiia A Cheremnykh, Rajeev Dattani, Vladimir V Egorov
The influenza virus polymerase complex is a promising target for new antiviral drug development. It is known that, within the influenza virus polymerase complex, the PB1 subunit region from the 1st to the 25th amino acid residues has to be is in an alpha-helical conformation for proper interaction with the PA subunit. We have previously shown that PB1(6-13) peptide at low concentrations is able to interact with the PB1 subunit N-terminal region in a peptide model which shows aggregate formation and antiviral activity in cell cultures...
January 8, 2018: Biophysical Chemistry
https://www.readbyqxmd.com/read/29328882/effects-of-salvia-miltiorrhiza-polysaccharides-on-lipopolysaccharide-induced-inflammatory-factor-release-in-raw264-7-cells
#20
Chao Han, Jinkai Yang, Pengyan Song, Xiao Wang, Wanyu Shi
This study investigated the anti-inflammatory effects and possible underlying mechanisms of Salvia miltiorrhiza polysaccharides (SMP) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The cytotoxicity of SMP was detected by the MTT method. The morphological change of RAW264.7 was observed by Diff-Quik staining. Enzyme-linked immunosorbent assay was used to evaluate the production of cytokines in LPS-induced RAW264.7 cells. The nitric oxide (NO) kit assay detected the NO release from LPS-induced RAW264...
January 2018: Journal of Interferon & Cytokine Research
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