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Bone marrow concentration

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https://www.readbyqxmd.com/read/29138222/decitabine-enhances-targeting-of-aml-cells-by-cd34-progenitor-derived-nk-cells-in-nod-scid-il2rg-null-mice
#1
Jeannette Cany, Mieke W H Roeven, Janneke S Hoogstad-van Evert, Willemijn Hobo, Frans Maas, Rosalia Franco Fernandez, Nicole M A Blijlevens, Walter J van der Velden, Gerwin Huls, Joop H Jansen, Nicolaas P M Schaap, Harry Dolstra
Combining NK cell adoptive transfer with hypomethylating agents (HMA) is an attractive therapeutic approach for patients with acute myeloid leukemia (AML). However, data regarding the impact of HMA on NK cell functionality are mostly derived from in vitro studies with high non-clinical relevant drug concentrations. Here, we report a comparative study of azacitidine and decitabine in combination with allogeneic NK cells generated from CD34(+) hematopoietic stem and progenitor cells (HSPC-NK cells) in in vitro and in vivo AML models...
November 14, 2017: Blood
https://www.readbyqxmd.com/read/29134323/single-stage-treatment-of-infected-tibial-non-unions-and-osteomyelitis-with-bone-marrow-granulocytes-precursors-protecting-bone-graft
#2
Philippe Hernigou, Arnaud Dubory, Yasuhiro Homma, Charles Henri Flouzat Lachaniette, Nathalie Chevallier, Helene Rouard
PURPOSE: Infected non-unions present a clinical challenge, especially with risk of recurrent infection. Bone marrow contains granulocyte precursors identified in vitro as colony forming units-granulocyte macrophage (CFU-GM) have a prophylactic action against infection. We therefore tested the hypothesis that bone marrow concentrated granulocytes precursors added to a standard bone graft could decrease the risk of recurrence of infection when single-stage treatment of infected tibial non-unions is performed with bone graft...
November 13, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/29132091/effect-of-anti-diabetic-drugs-on-bone-metabolism-evidence-from-preclinical-and-clinical-studies
#3
REVIEW
Mohammad Adil, Rashid Ali Khan, Abul Kalam, Shiva Kumar Venkata, Amit Dattatraya Kandhare, Pinaki Ghosh, Manju Sharma
Diabetes mellitus is associated with abnormal bone health and an increased risk of fracture even though patients have normal or higher BMD. The mechanisms behind diabetes mellitus- induced various skeletal disorders remain unclear. Anti-diabetic drugs may have negative or positive impact on bone metabolism. For instance, thiazolidinediones increases the bone loss and risk of fracture possibly through PPARγ activation in bone marrow cells and hamper osteoblastogenesis via decreasing Runx2 transcription factor, IGF-1 and Wnt signalling pathways...
May 25, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/29131717/in-vitro-effects-of-high-intensity-laser-photobiomodulation-on-equine-bone-marrow-derived-mesenchymal-stem-cell-viability-and-cytokine-expression
#4
Frances J Peat, Aimee C Colbath, Lori M Bentsen, Laurie R Goodrich, Melissa R King
OBJECTIVE: This study aimed to examine the influence of neodymium-doped yttrium aluminum garnet (Nd:YAG) laser irradiation on equine bone marrow-derived mesenchymal stem cell (MSC) viability, proliferation, and cytokine expression in vitro. BACKGROUND: Photobiomodulation of cells using monochromatic light is a technique designed to influence cellular processes. Previous studies have shown dose-dependent effects of low-level laser irradiation on cell proliferation and cytokine expression in a range of cell types and species...
November 13, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/29127762/anti-invasive-and-antiproliferative-effects-of-pleurotus-ostreatus-extract-on-acute-leukemia-cell-lines
#5
Alireza Ebrahimi, Amir Atashi, Masoud Soleimani, Maedeh Mashhadikhan, Ahmadreza Barahimi, Amirhossein Maghari
BACKGROUND: Currently, mushrooms have been used in traditional and folk medicines for their therapeutic activities, such as antibiotic, antitumor, anti-inflammatory, anticancer, antileukemic and immunomodulatory actions. This investigation evaluates the anti-invasive, antiproliferative and cytotoxic effects of Pleurotus ostreatus (Pleurotaceae) on leukemia cell lines. METHODS: The proliferation of KG-1 cells was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay after treatment with gradient dilutions of P...
November 11, 2017: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/29126349/bone-marrow-aspirate-concentrate-for-cartilage-defects-of-the-knee-from-bench-to-bedside-evidence
#6
Eric J Cotter, Kevin C Wang, Adam B Yanke, Susan Chubinskaya
Objective To critically evaluate the current basic science, translational, and clinical data regarding bone marrow aspirate concentrate (BMAC) in the setting of focal cartilage defects of the knee and describe clinical indications and future research questions surrounding the clinical utility of BMAC for treatment of these lesions. Design A literature search was performed using the PubMed and Ovid MEDLINE databases for studies in English (1980-2017) using keywords, including ["bone marrow aspirate" and "cartilage"], ["mesenchymal stem cells" and "cartilage"], and ["bone marrow aspirate" and "mesenchymal stem cells" and "orthopedics"]...
November 1, 2017: Cartilage
https://www.readbyqxmd.com/read/29121823/effect-of-tumor-necrosis-factor-alpha-dose-and-exposure-time-on-tumor-necrosis-factor-induced-gene-6-activation-by-neonatal-and-adult-mesenchymal-stromal-cells
#7
Shiva Hamidian Jahromi, Yunqing Li, John E Davies
Tumor necrosis factor (TNF)-stimulated gene 6 (TSG-6) is a major anti-inflammatory mediator released by activated mesenchymal stromal cells (MSCs). Neonatal MSCs are considered more metabolically active than cells derived from adult tissues, and potentially less heterogeneous. We hypothesized that a TNF-α activated neonatal MSC population (human umbilical cord perivascular cells-HUCPVCs) would show an enhanced level of TSG-6 activation compared to adult bone marrow MSCs (BMMSCs). Thus, we stimulated HUCPVCs, and both human and mouse BMMSCs with 1, 10, 50, and 100ng/mL of recombinant TNF-α over various exposure times...
November 9, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29121817/stromal-cell-derived-factor-1%C3%AE-promotes-endothelial-colony-forming-cell-migration-through-the-ca2-dependent-activation-of-the-extracellular-signal-regulated-kinase-1-2-and-phosphoinositide-3-kinase-akt-pathways
#8
Estella Zuccolo, Christian Andrea Di Buduo, Francesco Lodola, Stefania Orecchioni, Giorgia Scarpellino, Dlzar Ali Kheder, Valentina Poletto, Germano Guerra, Francesco Bertolini, Alessandra Balduini, Vittorio Rosti, Francesco Moccia
Stromal derived factor-1α (SDF-1α) drives endothelial colony forming cell (ECFC) homing and incorporation within neovessels, thereby restoring tissue perfusion in ischemic tissues and favouring tumor vascularization and metastasis. SDF-1α stimulates ECFC migration by activating the Gi-protein coupled receptor, CXCR4, and then engaging the phosphoinositide 3-kinase (PI3K)/AKT signalling pathway. Sporadic evidence showed that SDF-1α may also act through an increase in intracellular Ca2+ concentration ([Ca2+]i) in bone marrow-derived hematopoietic progenitor cells and fully differentiated endothelial cells...
November 9, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29119394/effects-of-bone-marrow-multipotent-mesenchymal-stromal-cells-and-their-secretion-products-on-the-cellular-composition-of-the-thymus-and-spleen-of-female-wistar-rats-with-experimental-chronic-inflammation-of-the-internal-genitals
#9
T I Dergacheva, A V Shurlygina, E V Mel'nikova, O B Gritsyk, M V Tenditnik, O V Poveshchenko, V I Konenkov
The effects of bone marrow multipotent mesenchymal stromal cells and their secretion products on the subpopulation composition of thymic and splenic lymphocytes were studied in female Wistar rats with experimental chronic inflammatory process in the internal genitals. Stromal cells and medium conditioned by these cells in different administration routes (intravenous or lymphotropic injection) produces different modulating effect on blood leukocyte count and on subpopulation composition of the splenic and thymic lymphocytes...
November 9, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29115467/coenzyme-q10-promotes-osteoblast-proliferation-and-differentiation-and-protects-against-ovariectomy-induced-osteoporosis
#10
Delu Zheng, Chenli Cui, Meng Yu, Xiang Li, Lu Wang, Xinyan Chen, Yichen Lin
Coenzyme Q10 (CoQ10) is a fat‑soluble vitamin‑like substance used for the treatment of a variety of disorders, including osteoporosis. The exact mechanism underlying CoQ10‑mediated protection against osteoporosis remains to be elucidated. The present study aimed to evaluate the effect of CoQ10 on osteoblastic cell proliferation and differentiation, and therapeutic effects on a rat model of osteoporosis. Following treatment with different concentrations of CoQ10, cell proliferation and differentiation of rat bone marrow stromal cells (BMSCs), and expression of osteoblastogenic markers, were measured...
October 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29115171/sika-pilose-antler-type-i-collagen-promotes-bmsc-differentiation-via-the-erk1-2-and-p38-mapk-signal-pathways
#11
Yanshuang Wang, Su Luo, Dafang Zhang, Xiaobo Qu, Yinfeng Tan
CONTEXT: Sika pilose antler type I collagen (SPC-I) have been reported to promote bone marrow mesenchymal stem cell (BMSC) proliferation and differentiation. However, the underlying mechanism is still unclear. OBJECTIVE: This study investigates the molecular mechanisms of SPC-I on the BMSC proliferation and differentiation of osteoblast (OB) in vitro. MATERIAL AND METHODS: The primary rat BMSC was cultured and exposed to SPC-I at different concentrations (2...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/29113298/il-8-gro-and-mcp-1-produced-by-hepatocellular-carcinoma-microenvironment-determine-the-migratory-capacity-of-human-bone-marrow-derived-mesenchymal-stromal-cells-without-affecting-tumor-aggressiveness
#12
Juan Bayo, Alejandrina Real, Esteban J Fiore, Mariana Malvicini, Leonardo Sganga, Marcela Bolontrade, Oscar Andriani, Carolina Bizama, Cristóbal Fresno, Osvaldo Podhajcer, Elmer Fernandez, Manuel Gidekel, Guillermo D Mazzolini, Mariana G García
New therapies are needed for advanced hepatocellular carcinoma (HCC) and the use of mesenchymal stromal cells (MSCs) carrying therapeutic genes is a promising strategy. HCC produce cytokines recruiting MSCs to the tumor milieu and modifying its biological properties. Our aim was to study changes generated on human MSCs exposed to conditioned media (CM) derived from human HCC fresh samples and xenografts. All CM shared similar cytokines expression pattern including CXCL1-2-3/GRO, CCL2/MCP-1 and CXCL8/IL-8 being the latter with the highest concentration...
October 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/29113211/aberrant-expression-of-cd133-and-cd82-in-patients-with-pediatric-acute-lymphoblastic-leukemia-and-the-clinical-significance
#13
Hongyan Ji, Li Chen, Yunpeng Dai, Xiaojun Sun, Xiuli Li, Qi Wang, Daoxin Ma, Dongdong Du, Ping Zhao, Yulin Wang
Cluster of differentiation (CD)133 is considered to be a marker of leukemia stem cells (LSCs), which are one of the primary causes of occurrence, drug resistance and relapse of acute lymphoblastic leukemia (ALL). CD82, an adhesion molecule, performs an important role in the interaction between LSCs and their niche. The purpose of the present study was to assess CD133 and CD82 expression in patients with pediatric ALL, and to evaluate the association with the clinical data. Using flow cytometric assessment and reverse transcription-polymerase chain reaction, CD133 and CD82 expression levels were measured in the bone marrow (BM) of 37 patients with newly diagnosed (ND) pediatric ALL [ALL-ND; 30 B-cell-ALL (B-ALL) and 7 T-cell-ALL (T-ALL)], in 22 patients with complete remission pediatric ALL (ALL-CR) and in 16 age-matched children without BM disease...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29112682/cross-coupling-effects-of-silencing-of-cyclooxygenase-2-cox-2-aggrecanase-1-and-over-expressed-insulin-like-growth-factor-1-igf-1-in-an-osteoarthritis-animal-model
#14
Zhao Zhang, Xiaofei Li, Heng Huang, Guozhong Wang, Zhigang Qu, Haining Zhang
BACKGROUND This study aimed to observe the effect of lentivirus-mediated cyclooxygenase-2 and aggrecanase-1 silencing and insulin-like growth factor-1 overexpression in human bone marrow mesenchymal stem cells after injection into model osteoarthritic knees. MATERIAL AND METHODS Using genetic recombination technique, the genes of cyclooxygenase-2, aggrecanase-1, and insulin-like growth factor-1 were recombined into the lentiviral vectors, and we transfected the human bone marrow stem cells in vitro. The BMSC transfected with lentivirus without genes served as a blank-virus group, and saline was used for another control group...
November 7, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29110360/enhancing-engineered-vascular-networks-in-vitro-and-in-vivo-the-effects-of-igf1-on-vascular-development-and-durability
#15
Claudia C Friedrich, Yunfeng Lin, Alexander Krannich, Yinan Wu, Joseph P Vacanti, Craig M Neville
OBJECTIVES: Creation of functional, durable vasculature remains an important goal within the field of regenerative medicine. Engineered biological vasculature has the potential to restore or improve human tissue function. We hypothesized that the pleotropic effects of insulin-like growth factor 1 (IGF1) would enhance the engineering of capillary-like vasculature. MATERIALS AND METHODS: The impact of IGF1 upon vasculogenesis was examined in in vitro cultures for a period of up to 40 days and as subcutaneous implants within immunodeficient mice...
November 7, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29109351/chronological-differential-effects-of-pro-inflammatory-cytokines-on-rankl-induced-osteoclast-differentiation-of-canine-bone-marrow-derived-macrophages
#16
Suranji Wijekoon, Eugene C Bwalya, Jing Fang, Sangho Kim, Kenji Hosoya, Masahiro Okumura
The aim of this study was to investigate osteoclastogenic properties of inflammatory cytokines at different time-points of osteoclastogenesis. Bone marrow-derived macrophages from five healthy dogs were stimulated with the macrophage colony-stimulating factor, receptor activator of nuclear factor-κB ligand and inflammatory cytokines such as interleukin (IL)-1β, tumor necrosis factor (TNF)-α and IL-17. Osteoclasts (OC) formation and function were enhanced with TNF-α regardless of temporal differences. But in contrast, IL-1β suppressed the osteoclastogenesis at early phase of the process while upregulating at the late phase...
November 3, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29109286/circadian-clock-cryptochrome-proteins-regulate-autoimmunity
#17
Qi Cao, Xuan Zhao, Jingwen Bai, Sigal Gery, Haibo Sun, De-Chen Lin, Qi Chen, Zhengshan Chen, Lauren Mack, Henry Yang, Ruishu Deng, Xianping Shi, Ling-Wa Chong, Han Cho, Jianjun Xie, Quan-Zhen Li, Markus Müschen, Annette R Atkins, Christopher Liddle, Ruth T Yu, Serhan Alkan, Jonathan W Said, Ye Zheng, Michael Downes, Ronald M Evans, H Phillip Koeffler
The circadian system regulates numerous physiological processes including immune responses. Here, we show that mice deficient of the circadian clock genes Cry1 and Cry2 [Cry double knockout (DKO)] develop an autoimmune phenotype including high serum IgG concentrations, serum antinuclear antibodies, and precipitation of IgG, IgM, and complement 3 in glomeruli and massive infiltration of leukocytes into the lungs and kidneys. Flow cytometry of lymphoid organs revealed decreased pre-B cell numbers and a higher percentage of mature recirculating B cells in the bone marrow, as well as increased numbers of B2 B cells in the peritoneal cavity of Cry DKO mice...
November 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29109124/endogenous-calcitriol-synthesis-controls-the-humoral-ige-response-in-mice
#18
Juliane Lindner, Sebastian Rausch, Sandra Treptow, Kerstin Geldmeyer-Hilt, Tina Krause, René St-Arnaud, Alice Arabian, Andreas Radbruch, Susanne Hartmann, Margitta Worm, Guido Heine
The vitamin D receptor participates in the control of IgE class-switch recombination in B cells. The physiologic vitamin D receptor agonist, 1,25(OH)2D3 (calcitriol), is synthesized by the essential enzyme 25-hydroxyvitamin D3-1α-hydroxylase (CYP27B1), which can be expressed by activated immune cells. The role of endogenous calcitriol synthesis for the regulation of IgE has not been proven. In this study, we investigated IgE-responses in Cyp27b1-knockout (KO) mice following sensitization to OVA or intestinal infection with Heligmosomoides polygyrus Specific Igs and plasmablasts were determined by ELISA and ELISpot, Cyp27b1 expression was measured by quantitative PCR...
November 6, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29108480/short-term-homing-of-hyaluronan-primed-cells-therapeutic-implications-for-osteoarthritis-treatment
#19
Giovanna Desando, Isabella Bartolotti, Carola Cavallo, Antonella Schiavinato, Cynthia Secchieri, Elizaveta Kon, Giuseppe Filardo, Maurizio Paro, Brunella Grigolo
The evaluation of key factors modulating cell homing following transplantation can provide new insights in the comprehension of unsolved biological questions about the use of cell therapies for osteoarthritis (OA). The main purpose of this in vivo study was to investigate the biodistribution of an intra-articular (IA) injection of mesenchymal stromal cells (MSCs) and bone marrow concentrate (BMC) in a rabbit OA model, and whether the additional use of sodium hyaluronate (HA) could modulate their migration and promote joint repair...
November 6, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29101872/evaluation-of-the-bisphosphonate-effect-on-stem-cells-derived-from-jaw-bone-and-long-bone-rabbit-models-a-pilot-study
#20
Jun-Beom Park, Seung-Hyun Cho, InSoo Kim, Won Lee, Seung-Hwan Kang, Heesung Kim
BACKGROUND AND OBJECTIVE: Bisphosphonates have been widely used and the number of patients experiencing medication-related osteonecrosis of the jaw (MRONJ) has been increasing. This study was designed to evaluate the effect of zoledronate on stem cells derived from different tissues. DESIGN: Stem cells derived from four different tissues were compared using rabbit models (JPO: periosteum from the jaw bone (mandible), JBM: bone marrow from the jaw bone, LPO: periosteum from long bone (tibia), and LBM: bone marrow from long bone)...
October 31, 2017: Archives of Oral Biology
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