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https://www.readbyqxmd.com/read/29777205/oviduct-epithelium-induces-interferon-tau-in-bovine-day-4-embryos-which-generates-an-anti-inflammatory-response-in-immune-cells
#1
Anup K Talukder, Mohammad B Rashid, Mohamed S Yousef, Kazuya Kusama, Takashi Shimizu, Masayuki Shimada, Susan S Suarez, Kazuhiko Imakawa, Akio Miyamoto
Recent studies indicate that communication between the bovine embryo and the mother begins in the oviduct. Here, we aimed to investigate the effect of embryos on bovine oviducts for their immune responses using an in vitro model. First, zygotes were cultured with or without bovine oviduct epithelial cells (BOECs) for 4 days, when embryos had reached the 16-cell stage. At that time, we detected interferon-tau (IFNT) in embryos co-cultured with BOECs, but not in embryos cultured alone. Next, peripheral blood mononuclear cells (PBMCs) were incubated either in media from embryo alone cultures or from co-cultures of embryos with BOECs...
May 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29774490/higher-activation-of-the-interferon-gamma-signaling-pathway-in-systemic-lupus-erythematosus-patients-with-a-high-type-i-ifn-score-relation-to-disease-activity
#2
Manman Liu, Junli Liu, Shumeng Hao, Ping Wu, Xiaoyan Zhang, Yichuan Xiao, Gengru Jiang, Xinfang Huang
Increased IFN-γ levels have been associated with systemic lupus erythematosus (SLE). However, the relationships among IFN-γ, type I interferons (IFNs) and clinical features have not been extensively studied. Peripheral blood samples from 44 SLE patients and 36 healthy donors (HDs) were collected. Quantitative real-time PCR was used to assess the mRNA expression of IFNG, type II IFN-inducible genes (IRF1, GBP1, CXCL9, CXCL10, and SERPING1, which are used for the type II IFN score), type I IFN-inducible genes (IRF7, MX1, ISG15, and ISG20, which are used for the type I IFN score), TBX21, and EOMES in peripheral blood mononuclear cells...
May 17, 2018: Clinical Rheumatology
https://www.readbyqxmd.com/read/29769653/isg15-in-antiviral-immunity-and-beyond
#3
REVIEW
Yi-Chieh Perng, Deborah J Lenschow
The host response to viral infection includes the induction of type I interferons and the subsequent upregulation of hundreds of interferon-stimulated genes. Ubiquitin-like protein ISG15 is an interferon-induced protein that has been implicated as a central player in the host antiviral response. Over the past 15 years, efforts to understand how ISG15 protects the host during infection have revealed that its actions are diverse and pathogen-dependent. In this Review, we describe new insights into how ISG15 directly inhibits viral replication and discuss the recent finding that ISG15 modulates the host damage and repair response, immune response and other host signalling pathways...
May 16, 2018: Nature Reviews. Microbiology
https://www.readbyqxmd.com/read/29758929/mir-370-regulates-isg15-expression-and-influences-ifn-%C3%AE-sensitivity-in-hepatocellular-carcinoma-cells
#4
Zhuo Liu, Min Ma, Lei Yan, Shilin Chen, Sha Li, Darong Yang, Xiaohong Wang, Hua Xiao, Hongyu Deng, Haizhen Zhu, Chaohui Zuo, Man Xia
BACKGROUND: Interferon-α (IFN-α) is an adjuvant to chemotherapy and radiotherapy for hepatocellular carcinoma (HCC), but some HCC patients do not respond to treatment with IFN-α. METHODS: We performed loss-of-function and gain-of-function experiments to examine the role of ISG15 in the IFN-α sensitivity of LH86, HLCZ01, SMMC7721, and Huh7 cell lines and tumor samples. RESULTS: The overexpression of ISG15 reduced apoptosis in Huh7 and LH86 cells in the presence of IFN-α, whereas the shRNA-mediated knock down of ISG15 expression increased apoptosis in both Huh7 and LH86 cells...
May 5, 2018: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/29758230/vaccination-with-uv-inactivated-nodavirus-partly-protects-european-sea-bass-against-infection-while-inducing-few-changes-in-immunity
#5
Yulema Valero, Mokrani Djamal, Elena Chaves-Pozo, Marta Arizcun, Mustapha Oumouna, José Meseguer, M Ángeles Esteban, Alberto Cuesta
Developing viral vaccines through the ultraviolet (UV) inactivation of virus is promising technique since it is straightforward and economically affordable, while the resulting viruses are capable of eliciting an adequate antiviral immune response. Nodavirus (NNV) is a devastating virus that mainly affects European sea bass juveniles and larvae, causing serious economic losses in Mediterranean aquaculture. In this work, a potential vaccine consisting on UV-inactivated NNV (iNNV) was generated and administered to healthy juveniles of European sea bass to elucidate whether it triggers the immune response and improves their survival upon challenge...
May 11, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29743376/transmembrane-protein-pul50-of-human-cytomegalovirus-inhibits-isgylation-by-downregulating-ube1l
#6
Myoung Kyu Lee, Ye Ji Kim, Young-Eui Kim, Tae-Hee Han, Jens Milbradt, Manfred Marschall, Jin-Hyun Ahn
Interferon-stimulated gene (ISG) 15 encodes a ubiquitin-like protein that can be conjugated to proteins via an enzymatic cascade involving the E1, E2, and E3 enzymes. ISG15 expression and protein ISGylation modulate viral infection; however, the viral mechanisms regulating the function of ISG15 and ISGylation are not well understood. We recently showed that ISGylation suppresses the growth of human cytomegalovirus (HCMV) at multiple steps of the virus life cycle, and that the virus-encoded pUL26 protein inhibits protein ISGylation...
May 9, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29702095/evidence-that-interferon-tau-secreted-from-day-7-embryo-in-vivo-generates-anti-inflammatory-immune-response-in-the-bovine-uterus
#7
Mohammad B Rashid, Anup K Talukder, Kazuya Kusama, Shingo Haneda, Toshiro Takedomi, Hitomi Yoshino, Satoru Moriyasu, Motozumi Matsui, Masayuki Shimada, Kazuhiko Imakawa, Akio Miyamoto
Recent studies suggest that Day-7 bovine embryo starts to communicate with the uterine epithelium through interferon-tau (IFNT) signaling. However, immune modulatory role of IFNT in the uterus just after the embryo moves from the oviduct is unclear. We aimed to examine the hypothesis that Day-7 bovine embryo secretes IFNT in the uterus, which induces anti-inflammatory response in immune cells. The uterine flush (UF) with multiple embryos was collected from Day-7 donor pregnant cows and peripheral blood mononuclear cells (PBMCs) were then cultured in UF...
April 24, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29681409/recombinant-bcg-rv2645-elicits-enhanced-protective-immunity-compared-to-bcg-in-vivo-with-induced-isgylation-related-genes-and-th1-and-th17-responses
#8
Wei Luo, Zilu Qu, Lingyun Zhang, Yan Xie, Fengling Luo, Yang Tan, Qin Pan, Xiao-Lian Zhang
There is a need to develop protective vaccines against tuberculosis (TB). Recently, we identified an immunodominant T-cell antigen, Rv2645, from the region of deletion 13 (RD13) of M. tuberculosis (M. tb) H37Rv, which is absent in Bacille Calmette-Guérin (BCG). Here, a recombinant BCG expressing Rv2645, namely, BCG::Rv2645, was constructed. Compared to BCG, we found that BCG::Rv2645 improved the antigen presentation capacity of dendritic cells (DCs) and elicited much stronger Th1 and Th17 responses, higher CD44high CD62low effector memory CD4+ T cells (TEM ), and fewer T regulated cells (Treg) and regulatory B10 in mice...
April 19, 2018: Vaccine
https://www.readbyqxmd.com/read/29658965/a-comprehensive-transcriptome-analysis-of-skeletal-muscles-in-two-polish-pig-breeds-differing-in-fat-and-meat-quality-traits
#9
Katarzyna Piórkowska, Kacper Żukowski, Katarzyna Ropka-Molik, Mirosław Tyra, Artur Gurgul
Pork is the most popular meat in the world. Unfortunately, the selection pressure focused on high meat content led to a reduction in pork quality. The present study used RNA-seq technology to identify metabolic process genes related to pork quality traits and fat deposition. Differentially expressed genes (DEGs) were identified between pigs of Pulawska and Polish Landrace breeds for two the most important muscles (semimembranosus and longissimus dorsi). A total of 71 significant DEGs were reported: 15 for longissimus dorsi and 56 for semimembranosus muscles...
January 2018: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29626479/protein-isgylation-and-free-isg15-levels-are-increased-by-interferon-gamma-in-breast-cancer-cells
#10
Angeles C Tecalco-Cruz, Eduardo Cruz-Ramos
The induction of ISG15 by interferon (IFN)-α/β and subsequent protein ISGylation has been demonstrated in several cell types. However, regulation of free ISG15 levels and ISGylation by other IFNs and its implications in some carcinomas have not yet been completely evaluated. Here, we demonstrated that free ISG15 and ISGylation levels are enhanced by IFN-γ treatment in the estrogen receptor-α-positive and -negative breast cancer cells, MCF-7 and MDA-MB-231, respectively. Specifically, IFN-γ increases free ISG15 levels in the cytoplasm and ISGylation in the nucleus and cytoplasm, but in a manner distinct between MCF-7 and MDA-MB-231 cells...
April 4, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29625402/effect-of-tropical-thermal-stress-on-peri-implantation-immune-responses-in-cows
#11
Mohanned Naif Alhussien, Aarti Kamboj, Mustafa Ahmad Aljader, Bibhudatta S K Panda, Munna Lal Yadav, Lakshit Sharma, Seid Mohammed, Aasif Ahmad Sheikh, Masoud Lotfan, Rajeev Kapila, A K Mohanty, Ajay Kumar Dang
Pregnancy losses during the peri-implantation period cause a negative impact on the reproductive and economic performance of dairy herds. In this study, we investigated the possible immunological factors which may contribute to pregnancy loss during the peri-implantation period under different seasons of tropical conditions. Eighteen Karan Fries cows, six cows in each season (W: winter; HH: hot-humid; HD: hot-dry) were selected. These cows exhibited heat and were brought for artificial insemination (AI; day 0)...
March 29, 2018: Theriogenology
https://www.readbyqxmd.com/read/29621762/mifepristone-inhibits-the-migration-of-cervical-cancer-cells-by-inhibiting-exocrine-secretion
#12
Lin Sang, Xiaoyan Wang, Xingbo Zhao
Cervical cancer (CC) is one of the most common gynecological malignancies, and metastasis limits the use of surgical resection. Metapristone (MIF) was reported to suppress the proliferation and migration of several cancer cells. Exosomes play a variety of roles in cellular biological processes. The relation of exosomes and CC is less studied. Cell viability, apoptosis assay and migration assay was conducted in HeLa cells treated by MIF by CCK-8 kit, staining by Annexin V-fluorescein isothiocyanate and propidium iodide, and wound test respectively...
April 5, 2018: Pharmacology
https://www.readbyqxmd.com/read/29621736/antiviral-activity-of-porcine-interferon-delta-8-against-foot-and-mouth-disease-virus-in-vitro
#13
Shi-Fang Li, Jun-Jun Shao, Fu-Rong Zhao, Mei-Jiao Gong, Yin-Li Xie, Hui-Yun Chang, Yong-Guang Zhang
Foot-and-mouth disease (FMD) is one of the most devastating diseases affecting livestock. Since vaccines fail to provide protection until seven days post-vaccination, the application of anti-viral molecules is imperative for suppressing the spread of FMDV prior to development of an adaptive immune response. Interferons (IFNs) are effective for the host to fight FMDV infections; however, a novel type I IFNs, interferon delta (IFN-δ), has not been investigated for their antiviral effects against this virus. Thus, this study investigated FMDV infection, upon pre- and post-treatment with PoIFN-δ8...
April 2, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29580840/hiv-1-promotes-the-degradation-of-components-of-the-type-1-ifn-jak-stat-pathway-and-blocks-anti-viral-isg-induction
#14
Siobhan Gargan, Suaad Ahmed, Rebecca Mahony, Ciaran Bannan, Silvia Napoletano, Cliona O'Farrelly, Persephone Borrow, Colm Bergin, Nigel J Stevenson
Anti-retroviral therapy successfully suppresses HIV-1 infection, but fails to provide a cure. During infection Type 1 IFNs normally play an essential role in viral clearance, but in vivo IFN-α only has a modest impact on HIV-1 infection, suggesting its possible targeting by HIV. Here, we report that the HIV protein, Vif, inhibits effective IFN-α signalling via degradation of essential JAK/STAT pathway components. We found that STAT1 and STAT3 are specifically reduced in HEK293T cells expressing Vif and that full length, infectious HIV-1 IIIB strain promotes their degradation in a Vif-dependent manner...
April 2018: EBioMedicine
https://www.readbyqxmd.com/read/29555370/isg15-promotes-esophageal-squamous-cell-carcinoma-tumorigenesis-via-c-met-fyn-%C3%AE-catenin-signaling-pathway
#15
Hongyu Yuan, Wei Zhou, Yang Yang, Liyan Xue, Linxiu Liu, Yongmei Song
Esophageal squamous cell carcinoma (ESCC) is one of the most malignant tumors in China with a poor prognosis. Most ESCC patients were diagnosed at advanced stages, losing the opportunity for surgical excision. Hence, it remains a pressing work to identify biomarkers for early detection, prognosis prediction and targeting therapies in ESCC. Interferon-stimulated gene 15 (ISG15) encodes a 15-kDa protein, and is involved in the post-translational modification (PTMs) of multiple proteins. However, the molecular functions of ISG15 in ESCC remain unclear...
March 16, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29518394/interferon-stimulated-gene-15-promotes-cholesterol-efflux-by-activating-autophagy-via-the-mir-17-5p-beclin-1-pathway-in-thp-1-macrophage-derived-foam-cells
#16
Chong Huang, Xiao-Hua Yu, Xi-Long Zheng, Xiang Ou, Chao-Ke Tang
Macrophage autophagy contributes to the hydrolysis of cholesteryl ester into free cholesterol mainly for ATP-binding cassette transporter A1 (ABCA1)-dependent efflux. Interferon-stimulated gene 15 (ISG15) has been shown to regulate autophagy in multiple types of cells. The present study aimed to examine the effects of ISG15 on autophagy and cholesterol efflux in THP-1 macrophage-derived foam cells and to explore the underlying molecular mechanisms. Our results showed that overexpression of ISG15 promoted autophagy and cholesterol efflux and inhibited lipid accumulation without impact on ABCA1 expression...
May 15, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29515574/human-intestinal-epithelial-cells-release-antiviral-factors-that-inhibit-hiv-infection-of-macrophages
#17
Le Guo, Xi-Qiu Xu, Li Zhou, Run-Hong Zhou, Xu Wang, Jie-Liang Li, Jin-Biao Liu, Hang Liu, Biao Zhang, Wen-Zhe Ho
As a rich source of CD4+ T cells and macrophages, the gastrointestinal (GI) tract is a major target site for HIV infection. The interplay between GI-resident macrophages and intestinal epithelial cells (IECs) constitutes an important element of GI innate immunity against pathogens. In this study, we investigated whether human IECs have the ability to produce antiviral factors that can inhibit HIV infection of macrophages. We demonstrated that IECs possess functional toll-like receptor 3 (TLR3), the activation of which resulted in induction of key interferon (IFN) regulatory factors (IRF3 and IRF7), IFN-β, IFN-λ, and CC chemokines (MIP-1α, MIP-1β, RANTES), the ligands of HIV entry co-receptor CCR5...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29510721/heme-oxygenase-1-promoter-region-gt-n-polymorphism-associates-with-increased-neuroimmune-activation-and-risk-for-encephalitis-in-hiv-infection
#18
Alexander J Gill, Rolando Garza, Surendra S Ambegaokar, Benjamin B Gelman, Dennis L Kolson
BACKGROUND: Heme oxygenase-1 (HO-1) is a critical cytoprotective enzyme that limits oxidative stress, inflammation, and cellular injury within the central nervous system (CNS) and other tissues. We previously demonstrated that HO-1 protein expression is decreased within the brains of HIV+ subjects and that this HO-1 reduction correlates with CNS immune activation and neurocognitive dysfunction. To define a potential CNS protective role for HO-1 against HIV, we analyzed a well-characterized HIV autopsy cohort for two common HO-1 promoter region polymorphisms that are implicated in regulating HO-1 promoter transcriptional activity, a (GT)n dinucleotide repeat polymorphism and a single nucleotide polymorphism (A(-413)T)...
March 6, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29510252/antiviral-activity-of-transiently-expressed-mitochondrial-antiviral-signaling-adapter-mavs-orthologue-from-asian-seabass
#19
Rahul Krishnan, Jeena K, Zahoor Mushtaq, K U Shyam, Pani Prasad Kurcheti
The innate immune signaling adapter, Mitochondrial antiviral signaling protein (MAVS) coordinates the signals received from two independent RLRs (RIG-1 and MDA5) to induce IFN & interferon stimulatory genes (ISGs). In the present study, we report identification of an orthologue of MAVS from Lates calcarifer (LcMAVS) and its functional role in piscine RLR signaling. The LcMAVS-cDNA was cloned into pcDNA and transfected into SISS cells. LcMAVS was detected to be a 61KDa protein in western blot. Confocal microscopy demonstrated the mitochondrial localization of LcMAVS...
May 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29508932/genome-wide-expression-profiling-analysis-to-identify-key-genes-in-the-anti-hiv-mechanism-of-cd4-and-cd8-t-cells
#20
Lijie Gao, Yunqi Wang, Yi Li, Ya Dong, Aimin Yang, Jie Zhang, Fengying Li, Rongqiang Zhang
Comprehensive bioinformatics analyses were performed to explore the key biomarkers in response to HIV infection of CD4+ and CD8+ T cells. The numbers of CD4+ and CD8+ T cells of HIV infected individuals were analyzed and the GEO database (GSE6740) was screened for differentially expressed genes (DEGs) in HIV infected CD4+ and CD8+ T cells. Gene Ontology enrichment, KEGG pathway analyses, and protein-protein interaction (PPI) network were performed to identify the key pathway and core proteins in anti-HIV virus process of CD4+ and CD8+ T cells...
March 6, 2018: Journal of Medical Virology
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