Jian Shi, Xinwen Wang, Huaijun Zhu, Hui Jiang, Danxin Wang, Alexey Nesvizhskii, Hao-Jie Zhu
Russell and colleagues deserve credit for being the first to use a QconCAT standard to simultaneously quantify both the wild-type and mutant peptides of a protein (i.e., CYP2B6) (J. Proteome Res. 2013,12 (12), 5934-5942, DOI: 10.1021/pr400279u). However, the rationale of their study was entirely different from ours (J. Proteome Res. 2018, 17 (10), 3606-3612, DOI: 10.1021/acs.jproteome.8b00620). Their study focused on the quantification of individual drug-metabolizing enzymes and transporters, whereas ours developed a targeted proteomics method to determine the allele-specific protein expression (ASPE) of a gene and advocated the use of the ASPE imbalance as the phenotype for identifying cis-regulatory genetic variants of the gene...
January 7, 2019: Journal of Proteome Research