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https://www.readbyqxmd.com/read/28421058/antimicrobial-compounds-produced-by-vaginal-lactobacillus-crispatus-are-able-to-strongly-inhibit-candida-albicans-growth-hyphal-formation-and-regulate-virulence-related-gene-expressions
#1
Shuai Wang, Qiangyi Wang, Ence Yang, Ling Yan, Tong Li, Hui Zhuang
The female vaginal environment contains diverse microorganisms, and their interactions play significant roles in health and disease. Lactobacillus species are the predominant vaginal microorganisms in healthy women and relevant as a barrier to defense against pathogens, including Candida albicans. The yeast-to-hyphae transition is believed to be a determinant of C. albicans pathogenesis. In this study, we investigated the effects of vaginal isolates of L. crispatus (seven strains), L. gasseri (six strains), and L...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28421052/whole-genome-based-amplified-fragment-length-polymorphism-analysis-reveals-genetic-diversity-in-candida-africana
#2
Anuradha Chowdhary, Ferry Hagen, Cheshta Sharma, Abdullah M S Al-Hatmi, Letterio Giuffrè, Domenico Giosa, Shangrong Fan, Hamid Badali, Maria Rosa Felice, Sybren de Hoog, Jacques F Meis, Orazio Romeo
This study aimed at investigating the genetic diversity of a panel of Candida africana strains recovered from vaginal samples in different countries. All fungal strains were heterozygous at the mating-type-like locus and belonged to the genotype A of Candida albicans. Moreover, all examined C. africana strains lack N-acetylglucosamine assimilation and sequence analysis of the HXK1 gene showed a distinctive polymorphism that impair the utilization of this amino sugar in this yeast. Multi-locus sequencing of seven housekeeping genes revealed a substantial genetic homogeneity among the strains, except for the CaMPIb, SYA1 and VPS13 loci which contributed significantly to the classification of our set of C...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28391495/boric-acid-inhibition-of-trichophyton-rubrum-growth-and-conidia-formation
#3
Martin Schmidt
Trichophyton rubrum is a common human dermatophyte that is the causative agent of 80-93% of fungal infections of the skin and nails. While dermatophyte infections in healthy people are easily treatable with over-the-counter medications, such infections pose a higher risk for patients with compromised immune function and impaired regenerative potential. The efficacy of boric acid (BA) for the treatment of vaginal yeast infections prompted an investigation of the effect of BA on growth and morphology of T. rubrum...
April 8, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28385421/prevalence-of-candida-albicans-candida-dubliniensis-and-candida-africana-in-pregnant-women-suffering-from-vulvovaginal-candidiasis-in-argentina
#4
María Josefina Mucci, María Luján Cuestas, María Fernanda Landanburu, María Teresa Mujica
BACKGROUND: Vulvovaginal candidiasis (VVC) is a vulvovaginitis commonly diagnosed in gynecology care. In recent years, the taxonomy of the most important pathogenic Candida species, such as Candida albicans have undergone significant changes. AIMS: This study examined the prevalence of C. albicans, Candida africana, and Candida dubliniensis in vaginal specimens from 210 pregnant women suffering from vulvovaginitis or having asymptomatic colonization. METHODS: Phenotypic and molecular methods were used for the identification of the species...
April 3, 2017: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/28369145/polyethylene-glycol-functionalized-poly-lactic-acid-co-glycolic-acid-and-graphene-oxide-nanoparticles-induce-pro-inflammatory-and-apoptotic-responses-in-candida-albicans-infected-vaginal-epithelial-cells
#5
R Doug Wagner, Shemedia J Johnson, Zhixia Yan Danielsen, Jin-Hee Lim, Thilak Mudalige, Sean Linder
Mucous-penetrating nanoparticles consisting of poly lactic acid-co-glycolic acid (PLGA)-polyethylene glycol (PEG) could improve targeting of microbicidal drugs for sexually transmitted diseases by intravaginal inoculation. Nanoparticles can induce inflammatory responses, which may exacerbate the inflammation that occurs in the vaginal tracts of women with yeast infections. This study evaluated the effects of these drug-delivery nanoparticles on VK2(E6/E7) vaginal epithelial cell proinflammatory responses to Candida albicans yeast infections...
2017: PloS One
https://www.readbyqxmd.com/read/28314677/vulvovaginal-candidiasis-etiology-symptomatology-and-risk-factors
#6
L Mtibaa, N Fakhfakh, A Kallel, S Belhadj, N Belhaj Salah, N Bada, K Kallel
OBJECTIVE: To determine epidemiological, clinical and mycological characteristics of vulvovaginal candidiasis (VVC) in Tunisian population and to evaluate predisposing factors. PATIENTS AND METHODS: In this retrospective study, 2160 vaginal swabs were performed over 2 years (January 2014-December 2015). It was carried out at the laboratory of Parasitology and Mycology, Rabta Hospital in Tunisia. After swab collecting, direct examination and culture on Sabouraud Chloramphenicol and Sabouraud Chloramphenicol Actidione media were implemented to research yeasts...
March 14, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/28293217/mechanistic-insights-underlying-tolerance-to-acetic-acid-stress-in-vaginal-candida-glabrata-clinical-isolates
#7
Diana V Cunha, Sara B Salazar, Maria M Lopes, Nuno P Mira
During colonization of the vaginal tract Candida glabrata cells are challenged with the presence of acetic acid at a low pH, specially when dysbiosis occurs. To avoid exclusion from this niche C. glabrata cells are expected to evolve efficient adaptive responses to cope with this stress; however, these responses remain largely uncharacterized, especially in vaginal strains. In this work a cohort of 18 vaginal strains and 2 laboratory strains (CBS138 and KUE100) were phenotyped for their tolerance against inhibitory concentrations of acetic acid at pH 4...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28263903/candida-and-its-dual-lifestyle-as-a-commensal-and-a-pathogen
#8
Mathieu Cauchie, Stefanie Desmet, Katrien Lagrou
Candida spp. are part of the normal vaginal microflora in 20-30% of healthy women. However, if the balance between these yeasts and the host is disturbed, Candida spp. can cause vulvovaginal candidiasis (VVC), with Candida albicans being the major causative agent. Different studies have been performed in order to better understand Candida's dual lifestyle in the vagina. The potential of C. albicans to switch from the yeast cell morphology to its hyphal form is considered a key element in VVC pathogenesis. Candida spp...
March 2, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28124960/-investigation-of-in-vitro-metronidazole-resistance-in-the-clinical-isolates-of-trichomonas-vaginalis
#9
Hatice Ertabaklar, Senem Yaman Karadam, Erdoğan Malatyalı, Sema Ertuğ
Trichomonas vaginalis, a flagellated, urogenital anaerobic protozoon is reported as an important cause of vaginitis with a global distribution. Although metronidazole is the primary choice of drug for the treatment of trichomoniasis, the presence of resistant isolates from many different countries highlights the need of novel drugs for the treatment. Many studies from Turkey mostly dealing with the in vitro effects of compounds and natural products against T.vaginalis have been reported, however, only one study has been encountered searching the metronidazole resistance in a single T...
October 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28063567/vulvovaginite-d%C3%A3-pistage-et-prise-en-charge-de-la-trichomonase-de-la-candidose-vulvovaginale-et-de-la-vaginose-bact%C3%A3-rienne
#10
Julie van Schalkwyk, Mark H Yudin
OBJECTIF: Analyser les données probantes et formuler des recommandations quant au dépistage et à la prise en charge de la candidose vulvovaginale, de la trichomonase et de la vaginose bactérienne. ISSUES: Parmi les issues évaluées, on trouve l'efficacité de l'antibiothérapie, les taux de guérison en ce qui concerne les infections simples et compliquées, et les implications de ces pathologies pendant la grossesse. RéSULTATS: La littérature publiée a été récupérée par l'intermédiaire de recherches menées dans MEDLINE, EMBASE, CINAHL et The Cochrane Library en juin 2013 au moyen d'un vocabulaire contrôlé (p...
December 2016: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
https://www.readbyqxmd.com/read/27915337/quercetin-assists-fluconazole-to-inhibit-biofilm-formations-of-fluconazole-resistant-candida-albicans-in-in-vitro-and-in-vivo-antifungal-managements-of-vulvovaginal-candidiasis
#11
Mei Gao, Hui Wang, LiJuan Zhu
BACKGROUND: Vulvovaginal candidiasis (VVC) is a common gynecological disease. Candida albicans is believed to be mainly implicated in VVC occurrence, the biofilm of which is one of the virulence factors responsible for resistance to traditional antifungal agents especially to fluconazole (FCZ). Quercetin (QCT) is a dietary flavonoid and has been demonstrated to be antifungal against C. albicans biofilm. METHODS: 17 C. albicans isolates including 15 clinical ones isolated from VVC patients were employed to investigate the effects of QCT and/or FCZ on the inhibition of C...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27847193/extremely-low-gestational-age-and-very-low-birthweight-for-gestational-age-are-risk-factors-for-autism-spectrum-disorder-in-a-large-cohort-study-of-10-year-old-children-born-at-23-27-weeks-gestation
#12
Robert M Joseph, Steven J Korzeniewski, Elizabeth N Allred, T Michael O'Shea, Tim Heeren, Jean A Frazier, Janice Ware, Deborah Hirtz, Alan Leviton, Karl Kuban
BACKGROUND: No prospective cohort study of high-risk children has used rigorous exposure assessment and optimal diagnostic procedures to examine the perinatal antecedents of autism spectrum disorder separately among those with and without cognitive impairment. OBJECTIVE: We sought to identify perinatal factors associated with increased risk for autism spectrum disorder with and without intellectual disability (intelligence quotient <70) in children born extremely preterm...
March 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27833516/utility-of-laboratory-diagnosis-for-confirmation-of-the-syndromic-case-management-in-married-indian-women-with-vaginal-discharge
#13
Prabhav Aggarwal, Sonali Bhattar, Satyendra Kumar Sahani, Preena Bhalla
OBJECTIVE: WHO and NACO recommend treatment of STIs/RTIs on the basis syndromic case management (SCM), even without laboratory confirmation, which may lead to over-treatment. Thus, this study was conducted to evaluate the utility of laboratory diagnosis for confirmation of patients with vaginal discharge diagnosed on the basis of SCM. METHODOLOGY: 234 married women in reproductive age group, diagnosed as having vaginal discharge syndrome were included. Normal saline wet-mount slide preparations were made for detection of motile trichomonads...
October 2016: International Journal of Health Sciences
https://www.readbyqxmd.com/read/27788740/-study-on-the-production-of-igg-derived-from-vaginal-epithelial-cells-and-the-effect-of-anti-candida-albicans
#14
X X Niu, T Li, Z H Liu
Objective: To investigate the function of IgG secreted by vaginal epithelial cells in natural resistance to vulvovaginal candidiasis. Methods: (1)Immunohistochemical method was used to determine the expression of IgG secreted by normal vaginal epithelial cells VK2/E6E7.(2)Samples were divided into three groups by different proportions of VK2/E6E7 cells to Candida albicans ,including Candida albicans: VK2/E6E7 cells were 1∶10, 1∶1[yeast+ cells(1∶10)group and yeast+ cells(1∶1)group]and VK2/E6E7 cells as blank control group...
October 25, 2016: Zhonghua Fu Chan Ke za Zhi
https://www.readbyqxmd.com/read/27751723/antifungal-effects-of-lavandula-binaludensis-and-cuminum-cyminum-essential-oils-against-candida-albicans-strains-isolated-from-patients-with-recurrent-vulvovaginal-candidiasis
#15
M H Minooeianhaghighi, L Sepehrian, H Shokri
OBJECTIVE: Recurrent vulvovaginal candidiasis (RVVC), which affects approximately 5% of women of reproductive age, is defined as 4 or more episodes of symptomatic Candida vaginitis within a year. The purposes of this study were to determine the chemical compositions and antifungal susceptibility of Cuminum cyminum (C. cyminum) and Lavandula binaludensis (L. binaludensis) essential oils and their combination against Candida albicans (C. albicans) strains isolated from patients with RVVC...
October 14, 2016: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/27744307/genetic-diversity-of-the-hwp1-gene-and-his3-ef3-cdc3-microsatellites-and-antifungal-susceptibility-profiles-of-candida-albicans-isolates-from-yaound%C3%A3-hiv-infected-patients
#16
Thierry Kammalac Ngouana, Pascal Drakulovski, Donika Krasteva, Rufin Kuipou Toghueo, Charles Kouanfack, Jacques Reynes, Eric Delaporte, Fabrice Fekam Boyom, Michèle Mallié, Sébastien Bertout
The molecular epidemiology and the antifungal susceptibility profiles of Candida albicans are scarce in Cameroon. Authors studied the genetic diversity and the antifungal susceptibility of C. albicans isolates from Yaoundé HIV-infected patients. Clinical isolates were obtained by mycological diagnosis of oropharyngeal swabs, stools, urine, and vaginal swabs from patients. C. albicans isolates were confirmed by the Light cycler real-time PCR of the ITS1 region of the 5.8s ribosomal DNA. The ABC genotypes and the Hwp1 gene amplification were carried out with specific primers...
October 14, 2016: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/27741168/the-in-vitro-effectiveness-of-lactobacillus-fermentum-against-different-candida-species-compared-with-broadly-used-azoles
#17
Francesca Deidda, Angela Amoruso, Stefania Nicola, Teresa Graziano, Marco Pane, Serena Allesina, Elisabetta Raiteri, Mario Del Piano, Luca Mogna
GOALS: To investigate the possible use of Lactobacillus strains in the prophylaxis and/or adjuvant therapy of acute vulvovaginal candidiasis and other vaginal infections sustained by Candida yeasts. BACKGROUND: The incidence of Candida infections has substantially increased in recent years. Treatment of vaginal infections with lactobacilli has a long tradition, starting with Döderlein's description of the vaginal microbiota. MATERIALS AND METHODS: We assessed the activity of serially diluted fluconazole and miconazole (from 3 ng/mL to 1 mg/mL) against Candida strains...
November 2016: Journal of Clinical Gastroenterology
https://www.readbyqxmd.com/read/27741167/in-vitro-activity-of-lactobacillus-fermentum-lf5-against-different-candida-species-and-gardnerella-vaginalis-a-new-perspective-to-approach-mixed-vaginal-infections
#18
Francesca Deidda, Angela Amoruso, Serena Allesina, Marco Pane, Teresa Graziano, Mario Del Piano, Luca Mogna
GOALS: This study was undertaken to demonstrate the ability of Lactobacillus fermentum LF5 (DSM 32277) to inhibit in vitro different Candida species and Gardnerella vaginalis to weigh its potential effectiveness even in mixed vaginal infections. BACKGROUND: A wide female population is suffering from various vulvovaginal infections. These diseases are often associated with a decrease in the concentration of Lactobacilli in the vagina. Mixed vaginal infections represent >20% of women with vulvovaginal infection...
November 2016: Journal of Clinical Gastroenterology
https://www.readbyqxmd.com/read/27679703/microdilution-in-vitro-antifungal-susceptibility-patterns-of-candida-species-from-mild-cutaneous-to-bloodstream-infections
#19
Elham Rezazadeh, Azar Sabokbar, Maryam Moazeni, Mohammad Sadegh Rezai, Hamid Badali
BACKGROUND: Candida species, as opportunistic organisms, can cause various clinical manifestations, ranging from mild cutaneous infections to systemic candidiasis in otherwise healthy individuals. Remarkably, the incidence and mortality rates of candidemia have significantly increased worldwide, even after advances in medical interventions and the development of novel antifungal drugs. OBJECTIVES: Given the possible resistance to antifungal agents, susceptibility testing can be useful in defining the activity spectrum of antifungals and determining the appropriate treatment regime...
July 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27568869/sensitivity-of-candida-albicans-to-essential-oils-are-they-an-alternative-to-antifungal-agents
#20
E Bona, S Cantamessa, M Pavan, G Novello, N Massa, A Rocchetti, G Berta, E Gamalero
AIMS: Candida albicans is an important opportunistic pathogen, responsible for the majority of yeast infections in humans. Essential oils, extracted from aromatic plants, are well-known antimicrobial agents, characterized by a broad spectrum of activities, including antifungal properties. The aim of this work was to assess the sensitivity of 30 different vaginal isolated strains of C. albicans to 12 essential oils, compared to the three main used drugs (clotrimazole, fluconazole and itraconazole)...
December 2016: Journal of Applied Microbiology
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