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https://www.readbyqxmd.com/read/29793200/influence-of-auxochrome-group-in-disperse-dyes-bearing-azo-groups-as-chromophore-center-in-the-biotransformation-and-molecular-docking-prediction-by-reductase-enzyme-implications-and-assessment-for-environmental-toxicity-of-xenobiotics
#1
Jefferson Honorio Franco, Bianca F da Silva, Elisangela Franciscon G Dias, Alexandre A de Castro, Teodorico C Ramalho, Maria Valnice Boldrin Zanoni
Synthetic azo dyes have increasingly become a matter of great concern as a result of the genotoxic and mutagenic potential of the products derived from azo dye biotransformation. This work evaluates the manner in which reducing enzymes produced by Escherichia coli (E. coli) act on three disperse dyes bearing azo groups, namely Disperse Red 73 (DR 73), Disperse Red 78 (DR 78), and Disperse Red 167 (DR 167). UV-Vis spectrophotometry, high-performance liquid chromatography with diode array detector (HPLC-DAD), and liquid chromatography mass spectrometry (LC-MS/MS) were applied towards the identification of the main products...
May 21, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29793194/simultaneous-determination-of-18-psychoactive-agents-and-6-metabolites-in-plasma-using-lc-ms-ms-and-application-to-actual-plasma-samples-from-conscription-candidates
#2
Dongeun Park, Hyeyoung Choi, Moonhee Jang, Hyejin Chang, Sanghee Woo, Wonkyung Yang
In Korea, an increasing number of people attempt to evade military conscription by posing as mental health patients. To verify the authenticity of mental illness, there is a need to detect wide range of psychoactive agents in biological specimens of conscription candidates. In this study, we developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of 18 psychoactive agents and 6 metabolites in human plasma. The method was characterized by the use of a simple, fast and cheap protein precipitation as sample preparation, a rapid run time (11min) and a low volume of plasma sample (200μL)...
April 22, 2018: Forensic Science International
https://www.readbyqxmd.com/read/29793191/development-and-validation-of-a-prediction-model-for-functional-decline-in-older-medical-inpatients
#3
Toshihiko Takada, Shingo Fukuma, Yosuke Yamamoto, Yukio Tsugihashi, Hiroyuki Nagano, Michio Hayashi, Jun Miyashita, Teruhisa Azuma, Shunichi Fukuhara
OBJECTIVE: To prevent functional decline in older inpatients, identification of high-risk patients is crucial. The aim of this study was to develop and validate a prediction model to assess the risk of functional decline in older medical inpatients. METHODS: In this retrospective cohort study, patients ≥65 years admitted acutely to medical wards were included. The healthcare database of 246 acute care hospitals (n = 229,913) was used for derivation, and two acute care hospitals (n = 1767 and 5443, respectively) were used for validation...
May 17, 2018: Archives of Gerontology and Geriatrics
https://www.readbyqxmd.com/read/29793137/3-o-sulfation-of-heparin-leads-to-hepatotropism-and-longer-circulatory-half-life
#4
Colton M Miller, Yongmei Xu, Katrina M Kudrna, Blake E Hass, Brianna M Kellar, Andrew W Egger, Jian Liu, Edward N Harris
INTRODUCTION: Heparins are common blood anticoagulants that are critical for many surgical and biomedical procedures used in modern medicine. In contrast to natural heparin derived from porcine gut mucosa, synthetic heparins are homogenous by mass, polymer length, and chemistry. MATERIALS & METHODS: Stable cell lines expressing the human and mouse Stabilin receptors were used to evaluate endocytosis of natural and synthetic heparin. We chemoenzymatically produced synthetic heparin consisting of 12 sugars (dodecamers) containing 14 sulfate groups resulting in a non-3-O sulfated structure (n12mer)...
May 17, 2018: Thrombosis Research
https://www.readbyqxmd.com/read/29793099/carbon-monoxide-analysis-method-in-human-blood-by-airtight-gas-syringe-gas-chromatography-mass-spectrometry-ags-gc-ms-relevance-for-postmortem-poisoning-diagnosis
#5
S Oliverio, V Varlet
Carbon monoxide is one of the most abundant toxic air pollutants. Symptoms of a CO intoxication are non-specific, leading to a high number of misdiagnosed CO poisoning cases that are missing in the disease statistics. The chemical nature of the molecule makes it difficult to detect for long periods and at low levels, thus requiring a very accurate and sensitive method. Current methods capable of accurate and sensitive analyses are available, however an inconsistency between results and symptoms are frequently reported...
May 18, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29793032/phosphorylated-and-non-phosphorylated-hck-kinase-domains-produced-by-cell-free-protein-expression
#6
Kazushige Katsura, Yuri Tomabechi, Takayoshi Matsuda, Mayumi Yonemochi, Junko Mikuni, Noboru Ohsawa, Takaho Terada, Shigeyuki Yokoyama, Mutsuko Kukimoto-Niino, Chie Takemoto, Mikako Shirouzu
Since phosphorylation is involved in various physiological events, kinases and interacting factors can be potential targets for drug discovery. For the development and improvement of inhibitors from the point of view of mechanistic enzymology, a cell-free protein synthesis system would be advantageous, since it could prepare mutant proteins easily. However, especially in the case of protein kinase, product solubility remains one of the major challenges. To overcome this problem, we prepared a chaperone-supplemented extract from Escherichia coli BL21 cells harboring a plasmid encoding a set of chaperone genes, dnaK, dnaJ, and grpE...
May 21, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29793031/cloning-overexpression-and-purification-of-a-gene-of-unknown-function-of-prophage-loci-from-candidatus-liberibacter-asiaticus-the-destructive-bacterial-pathogen-of-huanglongbing-disease-in-citrus-plants
#7
Duangtip Sudhan, Thamrongjet Puttamuk, Supachai Vuttipongchaikij, Pitak Chuawong
Citrus Huanglongbing (HLB) or citrus greening is one of the most destructive diseases affecting citrus industry worldwide. The causal agent in Asia is a phloem-limited, Gram-negative bacterium, 'Candidatus Liberibacter asiaticus' (CLas). Within the genome of CLas lies prophage regions, classified as Type-A, B, C, and D. In particular, Type-D has been indicated to correlate with the blotchy-mottle symptoms of citrus trees. Here we reported the cloning, overexpression, and purification of the ORF1, an open reading frame from the partial Type-D region of CLas obtained from an infected lime tree (Citrus aurantifolia Swingle)...
May 21, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29793029/oxidative-degradation-of-atenolol-by-heat-activated-persulfate-kinetics-degradation-pathways-and-distribution-of-transformation-intermediates
#8
Dong Miao, Jianbiao Peng, Xiaohuan Zhou, Li Qian, Mengjie Wang, Li Zhai, Shixiang Gao
Atenolol (ATL) has been widely detected in wastewater and aquatic environment. Although satisfactory removal of ATL from wastewater could be achieved, the mineralization ratio is usually low, which may result in the accumulation of its transformation products in the effluent and cause additional ecological risk to the environment. The aim of this study is to explore the effectiveness of heat activated persulfate (PS) in the removal of ATL from wastewater. Influencing factors including temperature, PS dosage, solution pH, existence of NO3 - , Cl- , HCO3 - and Suwannee river fulvic acid (SRFA) were examined...
May 17, 2018: Chemosphere
https://www.readbyqxmd.com/read/29793028/cytosolic-distributions-of-highly-toxic-metals-cd-and-tl-and-several-essential-elements-in-the-liver-of-brown-trout-salmo-trutta-l-analyzed-by-size-exclusion-chromatography-and-inductively-coupled-plasma-mass-spectrometry
#9
Zrinka Dragun, Nesrete Krasnići, Nicol Kolar, Vlatka Filipović Marijić, Dušica Ivanković, Marijana Erk
Cytosolic distributions of nonessential metals Cd and Tl and seven essential elements among compounds of different molecular masses were studied in the liver of brown trout (Salmo trutta) from the karstic Krka River in Croatia. Analyses were done by size exclusion high performance liquid chromatography and high resolution inductively coupled plasma mass spectrometry. Common feature of Cd and Tl, as highly toxic elements, was their distribution within only two narrow peaks. The increase of cytosolic Cd concentrations was reflected in marked increase of Cd elution within low molecular mass peak (maximum at ∼15 kDa), presumably containing metallothioneins (MTs), which indicated successful Cd detoxification in brown trout liver under studied exposure conditions...
May 15, 2018: Chemosphere
https://www.readbyqxmd.com/read/29793015/right-atrial-anomalous-muscle-bundle-presenting-with-acute-superior-vena-cava-syndrome-and-pulmonary-embolism-surgical-management
#10
Jeko M Madjarov, Michael G Katz, Sophia Madjarova, Svetozar Madzharov, Frank R Arko, David W Miller, Francis Robicsek
BACKGROUND: An anomalous muscle bundle crossing the right atrial cavity represents a pathological finding with unproved clinical significance. This congenital anomaly may be difficult to recognize via echocardiography and could be confused with other intra-cavitary lesions. METHODS: We report a case of a 53-year-old female presented to the cardio-vascular service with acute superior vena cava syndrome and sub-massive pulmonary embolism. RESULTS: The patient underwent venography confirming superior vena cava stenosis...
May 21, 2018: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/29792999/does-solid-fusion-eliminate-rod-fracture-after-pedicle-subtraction-osteotomy-in-ankylosing-spondylitis-related-thoracolumbar-kyphosis
#11
Shi-Zhou Zhao, Bang-Ping Qian, Mu Qiao, Jun Jiang, Yong Qiu
BACKGROUND CONTEXT: Rod fracture (RF) has a negative impact on the surgical outcome of ankylosing spondylitis (AS) patients following lumbar pedicle subtraction osteotomy (PSO). However, there is a paucity of published studies analyzing the risk factors for RF in PSO-treated AS patients with thoracolumbar kyphosis. PURPOSE: To investigate the risk factors for RF after PSO for thoracolumbar kyphosis secondary to AS. STUDY DESIGN/SETTING: Retrospective single-center study...
May 21, 2018: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/29792998/bmi-and-gender-increase-risk-for-sacral-fractures-after-multilevel-instrumented-spinal-fusion-compared-to-bone-mineral-density-and-pelvic-parameters
#12
Stephan N Salzmann, Courtney Ortiz Miller, John A Carrino, Jingyan Yang, Jennifer Shue, Andrew A Sama, Frank P Cammisa, Federico P Girardi, Alexander P Hughes
BACKGROUND CONTEXT: Sacral fractures are a rare but potentially devastating complication. Long-fusion constructs including the sacrum that do not extend to the pelvis may result in sacral fractures. Besides established risk factors including gender, age and number of levels fused, body mass index (BMI), pelvic parameters and bone mineral density (BMD) have also been proposed as potential risk factors for postoperative sacral fractures. The literature supporting this, however, is limited...
May 21, 2018: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/29792981/probabilistic-non-carcinogenic-and-carcinogenic-risk-assessments-monte-carlo-simulation-method-of-the-measured-acrylamide-content-in-tah-dig-using-quechers-extraction-and-uhplc-ms-ms
#13
Parvin Eslami Shahrbabki, Bahador Hajimohammadi, Shahram Shoeibi, Mehdi Elmi, Arash Yousefzadeh, Gea Oliveri Conti, Margherita Ferrante, Maryam Amirahmadi, Yadolah Fakhri, Amin Mousavi Khaneghah
A modified "Quick, Easy, Cheap, Effective, Rugged, and Safe" QuEChERS in combination with Ultra-High Performance Liquid Chromatography/tandem mass spectrometry (UHPLC-MS/MS) method was optimized for the determination of acrylamide content in different types of tah-dig (rice, bread, and potato). Also, the non-carcinogenic and carcinogenic risks (target hazard quotient (THQ) and incremental lifetime cancer risk (ILCR)) due to ingestion of acrylamide via tah-dig in the adults and children were assessed by Monte Carlo simulation (MCS) method...
May 21, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29792964/a-family-30-glucurono-xylanase-from-bacillus-subtilis-lc9-expression-characterization-and-its-application-in-chinese-bread-making
#14
Yalan Guo, Zhen Gao, Jiaxing Xu, Siyuan Chang, Bin Wu, Bingfang He
A GH30-8 endoxylanase was identified from an environmental Bacillus subtilis isolate following growth selection on aspen wood glucuronoxylan. The putative endoxylanase was cloned for protein expression and characterization in the Gram-positive protease deficient protein expression host B. subtilis WB800. The extracellular activity obtained was 55 U/mL, which was 14.5-fold higher than that obtained with the native species. The apparent molecular mass of BsXyn30 was estimated as 43 kDa by SDS-PAGE. BsXyn30 showed an optimal activity at pH 7...
May 21, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29792946/investigation-of-nonalcoholic-fatty-liver-disease-induced-drug-metabolism-by-comparative-global-toxicoproteomics
#15
Ann-Yae Na, Jung Jae Jo, Ok Kwang Kwon, Riya Shrestha, Piljoung Cho, Kyu Min Kim, Sung Hwan Ki, Tae Hee Lee, Tae Won Jeon, Tae Cheon Jeong, Sangkyu Lee
Non-alcoholic fatty liver disease (NAFLD) includes conditions such as steatosis, non-alcoholic steatohepatitis, and ultimately hepatocellular carcinoma. Although the pathology of NAFLD is well-established, NAFLD-induced drug metabolism mediated by cytochrome P450 (CYP) in the liver has remained largely unexplored. Therefore, we investigated NAFLD-induced drug metabolism mediated by CYP by quantitative toxicoproteomics analysis. After administration of a methionine-choline deficient (MCD) diet to induce development of NAFLD, tandem mass tags-based liquid chromatography-tandem mass spectrometry analysis was conducted to investigate the dynamics of hepatic proteins...
May 21, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29792926/prediction-of-midlife-hand-osteoarthritis-in-young-men
#16
Karin Magnusson, Aleksandra Turkiewicz, Simon Timpka, Martin Englund
OBJECTIVES: Improved prediction modeling in osteoarthritis (OA) may encourage risk reduction through calculation of individual and population lifetime risks. There are currently no prediction models for hand OA. Thus, we aimed to i) develop a prediction model for hand OA in men and ii) to contrast its discriminative performance to a prediction model for lung cancer and chronic obstructive pulmonary disease (COPD). METHODS: We included 40 118 men aged 18 years undergoing mandatory conscription in Sweden 1969-70...
May 21, 2018: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/29792921/characterization-of-solution-stress-degradation-products-of-aliskiren-and-prediction-of-their-physicochemical-and-admet-properties
#17
Bhoopendra Singh Kushwah, Jugal Gupta, Dilip Kumar Singh, Moolchand Kurmi, Archana Sahu, Saranjit Singh
Forced degradation studies on aliskiren were carried out according to ICH and WHO guidelines. Six degradation products were formed in total in the solution state. Their separation among themselves and from the drug was successfully achieved on a C-18 column utilizing acetonitrile and phosphate buffer (pH 3.0) in the mobile phase, which was run in a gradient mode. To characterize them, a complete mass fragmentation pathway of the drug was first established with the help of MS/TOF and MSn data. This was followed by LC-MS/TOF studies on the degradation products...
May 21, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29792883/plasma-n-glycan-signatures-associate-with-features-of-inflammatory-bowel-diseases
#18
Florent Clerc, Mislav Novokmet, Viktoria Dotz, Karli R Reiding, Noortje de Haan, Guinevere S M Kammeijer, Hans Dalebout, Marco R Bladergroen, Frano Vukovic, Erdmann Rapp, Stephan R Targan, Gildardo Barron, Natalia Manetti, Anna Latiano, Dermot P B McGovern, Vito Annese, Gordan Lauc, Manfred Wuhrer
BACKGROUND & AIMS: Biomarkers are needed for early detection of Crohn's disease (CD) and ulcerative colitis (UC) or to predict patient outcomes. Glycosylation is a common and complex post-translational modification of proteins that affects their structure and activity. We compared plasma N-glycosylation profiles between patients with CD or UC and healthy individuals (controls). METHODS: We analyzed the total plasma N-glycomes of 2635 patients with inflammatory bowel diseases and 996 controls by mass spectrometry with a linkage-specific sialic acid derivatization technique...
May 21, 2018: Gastroenterology
https://www.readbyqxmd.com/read/29792881/the-temporal-association-of-robotic-surgical-diffusion-with-overtreatment-of-the-small-renal-mass
#19
Paras H Shah, Manaf A Alom, Bradley C Leibovich, R Houston Thompson, Robert G Uzzo, Louis R Kavoussi, Lee Richstone, Bimal Bhindi, Elizabeth B Habermann, Vidhu Joshi, Stephen A Boorjian
PURPOSE: To evaluate contemporary practice patterns in the management of small renal masses. METHODS: We identified 52,804 patients within the National Cancer Database diagnosed with a small renal mass (≤4cm) between 2010-2014. Utilization trends for active surveillance, ablation, and robotic, laparoscopic, and open surgical techniques were compared among all-comers, elderly patients (≥75 years) and individuals with competing health risks (Charlson index ≥2)...
May 21, 2018: Journal of Urology
https://www.readbyqxmd.com/read/29792853/preoperative-obstructive-sleep-apnea-screening-in-gynecologic-oncology-patients
#20
Ross F Harrison, Erin E Medlin, Chase B Petersen, Stephen L Rose, Ellen M Hartenbach, David M Kushner, Ryan J Spencer, Laurel W Rice, Ahmed N Al-Niaimi
BACKGROUND: Women with a gynecologic cancer tend to be older, obese, and postmenopausal, characteristics that are associated with an increased risk for obstructive sleep apnea. However, there is limited investigation regarding the condition's prevalence in this population or its impact on postoperative outcomes. In other surgical populations, patients with obstructive sleep apnea have been observed to be at increased risk for adverse postoperative events. OBJECTIVES: To estimate the prevalence of obstructive sleep apnea among gynecologic oncology patients undergoing elective surgery and to investigate for a relationship between obstructive sleep apnea and postoperative outcomes...
May 21, 2018: American Journal of Obstetrics and Gynecology
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