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JOURNAL ARTICLE
Angela M Develin, Brian Fuglestad
As a key component for NADPH oxidase 2 (NOX2) activation, the peripheral membrane protein p47phox translocates a cytosolic activating complex to the membrane through its PX domain. This study elucidates a potential regulatory mechanism of p47phox recruitment and NOX2 activation by inositol hexaphosphate (IP6). Through NMR, fluorescence polarization, and FRET experimental results, IP6 is shown to be capable of breaking the lipid binding and membrane anchoring events of p47phox -PX with low micromolar potency...
April 26, 2024: Biochemistry
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JOURNAL ARTICLE
Haidong Ding, Rina Wu
BACKGROUND: Chronic Obstructive Pulmonary Disease (COPD) is characterized by airflow limitation and inflammation resulting from genetic and environmental factors, notably cigarette smoke. Pyroptosis, a cell death process, is implicated in COPD, but its mechanisms are unclear. SHP2, a phosphatase, modulates inflammatory pathways, suggesting a role in COPD pathogenesis and potential therapeutic avenues. OBJECTIVE: This study investigates the mechanism by which SHP2 regulates cell pyroptosis in bronchial epithelial cells in COPD patients...
April 18, 2024: Alternative Therapies in Health and Medicine
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JOURNAL ARTICLE
Yaming Yang, Zheng Yan, Qi Xie, Yong Wang, Zhiying Liu, Min Lei
BACKGROUND: The purpose of this study is to observe LP45 (Lactobacillus plantarum 45) to investigate the mechanism by which LP45 attenuates oxidative stress-induced damage and regulates the osteoblast-osteoclast balance. MATERIALS AND METHODS: The oxidative stress level and osteoblast- and osteoclast-related proteins were detected by immunofluorescence staining, Western blotting, ROS fluorescent probe and ELISA. Osteoblast cell proliferation capacity was determined by the CCK-8 assay...
April 3, 2024: Aging
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JOURNAL ARTICLE
Kuo-Wei Chen, Yong-Ren Chen, Ling-Yu Yang, Ya-Wen Cheng, Sheng-Che Chou, Yi-Hsing Chen, Yi-Tzu Chen, Sung-Tsang Hsieh, Meng-Fai Kuo, Kuo-Chuan Wang
BACKGROUND AND OBJECTIVES: Hydrocephalus is characterized by progressive enlargement of cerebral ventricles, resulting in impaired microvasculature and cerebral hypoperfusion. This study aimed to demonstrate the microvascular changes in hydrocephalic rats and the effects of cerebrospinal fluid (CSF) release on cerebral blood flow (CBF). METHODS: On postnatal day 21 (P21), male Wistar rats were intracisternally injected with either a kaolin suspension or saline. On P47, Evan's ratio (ER) was measured using MRI...
March 21, 2024: Neurosurgery
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JOURNAL ARTICLE
Simon V N P Kuihon, Brodrick J Sevart, Colette A Abbey, Kayla J Bayless, Baoyu Chen
The actin cytoskeleton and reactive oxygen species (ROS) both play crucial roles in various cellular processes. Previous research indicated a direct interaction between two key components of these systems: the WAVE1 subunit of the WAVE regulatory complex (WRC), which promotes actin polymerization, and the p47phox subunit of the NADPH oxidase 2 complex (NOX2), which produces ROS. Here, using carefully characterized recombinant proteins, we find that activated p47phox uses its dual SH3 domains to bind to multiple regions within the WAVE1 and Abi2 subunits of the WRC, without altering WRC's activity in promoting Arp2/3-mediated actin polymerization...
March 1, 2024: Journal of Biological Chemistry
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JOURNAL ARTICLE
Rongna Liu, Lin Chen, Xin Zhao, Lili Bao, Ruixia Wei, Xiaohua Wu
OBJECTIVE: To explore the effect of MUC1 on recurrent implantation failure (RIF) and its molecular mechanism. METHODS: Bioinformation analysis was used to find possible molecular mechanisms of specific genes in the pathogenesis of RIF. The number of M1 and M2 macrophages was measured by flow cytometry. Immunohistochemical staining and western blotting were used to detect the expression of related proteins. Angiogenesis capacity was measured by cell tube-formation assay...
February 26, 2024: Aging
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JOURNAL ARTICLE
Fusheng Jiang, Chenglong Hua, Jieli Pan, Suyu Peng, Dandan Ning, Cheng Chen, Shiqing Li, Xiaohua Xu, Linyan Wang, Chunchun Zhang, Meiya Li
BACKGROUND & AIMS: The effect fraction of Bletilla striata (Thunb.) Reichb.f. (EFBS), a phenolic-rich extract, has significant protective effects on lipopolysaccharide (LPS)-induced acute lung injury (ALI), but its composition and molecular mechanisms are unclear. This study elucidated its chemical composition and possible protective mechanisms against LPS-induced ALI from an antioxidant perspective. METHODS: EFBS was prepared by ethanol extraction, enriched by polyamide column chromatography, and characterized using ultra-performance liquid chromatography/time-of-flight mass spectrometry...
November 4, 2023: Phytomedicine
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JOURNAL ARTICLE
Brian Alvarez, Chloe Balleste Delpierre
OBJECTIVES: Our study aimed to identify the potential causes for the low lung procurement rate in Argentina and to develop strategies to the actual problems in donor management focused on lung retrieval. MATERIALS AND METHODS: We performed a crosssectional study in which we analyzed data obtained from digital self-reported surveys conducted on professionals participating in the organ procurement process in Argentina. The surveys included questions on donor management, selection criteria, and resource availability involving increasing lung procurement...
January 2024: Experimental and Clinical Transplantation
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JOURNAL ARTICLE
Nisha Singh, Agustin Rolandelli, Anya J O'Neal, L Rainer Butler, Sourabh Samaddar, Hanna J Laukaitis-Yousey, Matthew Butnaru, Stephanie E Mohr, Norbert Perrimon, Joao H F Pedra
Although genetic manipulation is one of the hallmarks of model organisms, its applicability to non-model species has remained difficult due to our limited understanding of their fundamental biology. For instance, manipulation of a cell line originated from the black-legged tick Ixodes scapularis, an arthropod that serves as a vector for several human pathogens, has yet to be established. Here, we demonstrate the successful genetic modification of the commonly used tick ISE6 line through ectopic expression and clustered regularly interspaced palindromic repeats [(CRISPR)/CRISPR-associated protein 9 (Cas9)] genome editing...
February 21, 2024: MBio
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JOURNAL ARTICLE
Mingang Hao, Peixin Lu, Sarah Sotropa, Kanakaraju Manupati, Syn Kok Yeo, Jun-Lin Guan
Autophagy is a conserved cellular process, and its dysfunction is implicated in cancer and other diseases. Here, we employ an in vivo CRISPR screen targeting genes implicated in the regulation of autophagy to identify the Nsfl1c gene encoding p47 as a suppressor of human epidermal growth factor receptor 2 (HER2)+ breast cancer metastasis. p47 ablation specifically increases metastasis without promoting primary mammary tumor growth. Analysis of human breast cancer patient databases and tissue samples indicates a correlation of lower p47 expression levels with metastasis and decreased survival...
February 15, 2024: Cell Reports
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JOURNAL ARTICLE
Xiaoyu Liu, Yiting Shi, Rui Liu, Kangcheng Song, Lei Chen
Phagocyte NADPH oxidase, a protein complex with a core made up of NOX2 and p22 subunits, is responsible for transferring electrons from intracellular NADPH to extracellular oxygen1 . This process generates superoxide anions that are vital for killing pathogens1 . The activation of phagocyte NADPH oxidase requires membrane translocation and the binding of several cytosolic factors2 . However, the exact mechanism by which cytosolic factors bind to and activate NOX2 is not well understood. Here we present the structure of the human NOX2-p22 complex activated by fragments of three cytosolic factors: p47, p67 and Rac1...
February 14, 2024: Nature
#12
JOURNAL ARTICLE
Yu Li, Ailin Guo, Jianlei Liu, Lijuan Tang, Lide Su, Zonghong Liu
OBJECTIVE: Notch-1 is a signal regulatory protein with extensive effects in myeloid cells, but its role in aneurysms remains to be fully clarified. In this study, therefore, the aneurysm mouse model with myeloid-specific knockout of Notch-1 was established to observe the role of Notch-1 in aneurysm progression. METHODS AND RESULTS: The effect of Notch-1 was assessed by pathological staining and Western blotting. It was found that after myeloid-specific knockout of Notch-1 in the aneurysm mouse model, the area of aneurysms and the macrophage infiltration were significantly reduced, the damage to arterial elastic plates was significantly relieved, and the oxidative stress level significantly declined...
January 26, 2024: Aging
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JOURNAL ARTICLE
Xiuhong Lin, Xiaoyun Wang, Weilian Feng, Yan Wan, Jiani Chai, Feng Li, Mingtong Xu
INTRODUCTION: China now has the highest number of diabetes in the world. Angiotensin II (Ang II) causes insulin resistance by acting on the insulin signaling pathway of peripheral target tissues. However, its effect on islet β-cells remains unclear. The possible role of Angiotensin-(1-7) [Ang-(1-7)] as an antagonist to the effects of Ang II and in treating diabetes needs to be elucidated. OBJECTIVES: To assess the effects of Ang II and Ang-(1-7) on the function and growth of islet β cell line NIT-1, which is derived from the islets of non-obese diabetic/large T-antigen (NOD/LT) mice with insulinoma...
January 1, 2024: Current Diabetes Reviews
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JOURNAL ARTICLE
Elisa Karen Gutiérrez Rodríguez, Fernando Astudillo-Melgar, Violeta Larios, Francisco Bolívar, Adelfo Escalante, Martha Giles-Gómez
We report the sequence of the complete genome and associated plasmids of two Lactiplantibacillus plantarum isolates from the traditional Mexican pulque beverage assembled with a combination of PacBio and Illumina data. The resulting complete genome for strain LB1_P46 is 3,287,706 bp; for strain LB2_P47, the complete genome is 3,289,072 bp.
December 19, 2023: Microbiology Resource Announcements
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JOURNAL ARTICLE
Suzune Nakayama, Hisao Kondo
Two distinct p97ATPase-mediated membrane fusion pathways are required for Golgi and endoplasmic reticulum (ER) biogenesis; namely, the p97/p47 pathway and the p97/p37 pathway. p97(VCP)/p47 complex-interacting protein, p135 (VCIP135) is necessary for both of these pathways. Although VCIP135 is known to form a complex with p97 in the cytosol, the role of this complex in Golgi and ER biogenesis has remained unclear. In this study, we demonstrated that VCIP135 has two distinct p97-binding sites at its N- and C-terminal regions...
December 8, 2023: Journal of Biological Chemistry
#16
JOURNAL ARTICLE
Yukun Yuan, Jordan M Bailey, Gretchen M Rivera-Lopez, William D Atchison
The exact causes of Amyotrophic lateral sclerosis (ALS), a progressive and fatal neurological disorder due to loss of upper and/or lower motoneurons, remain elusive. Gene-environment interactions are believed to be an important factor in the development of ALS. We previously showed that in vivo exposure of mice overexpressing the human superoxide dismutase 1 (hSOD1) gene mutation (hSOD1G93A; G93A), a mouse model for ALS, to environmental neurotoxicant methylmercury (MeHg) accelerated the onset of ALS-like phenotype...
January 2024: Neurotoxicology
#17
JOURNAL ARTICLE
Haopeng Ye, Weidan Ren, Guiwei Liu, Qingjin Guo
OBJECTIVE: Currently, multiple studies have shown that long non-coding ribonucleic acid TUSC7 exerts an anti-tumor effect in a variety of cancers. However, the function and underlying regulatory mechanism of lncRNA TUSC7 in CRC remain unclear. METHODS: The relative fluorescence intensity of MMP9 in the cancer and in peritoneal tissues was measured by immunofluorescence. Peritoneal macrophages in BALB/c mice were sorted out using flow cytometry. The abdominal circumference of mice was measured...
December 5, 2023: Aging
#18
JOURNAL ARTICLE
Zhipeng Tang, Xiuqin Li, Nan Yin, Ming Zhao, Qingchuan Hu, Peiyuan Lv
PURPOSE: To investigate the influences and mechanism of erythropoietin (EPO) on the cognitive function of vascular dementia (VD) rats. METHODS: 1) Spatial memory capacity was assessed by Morris water maze test; 2) Pathological conditions of brain tissues were detected by hematoxylin-eosin (HE) staining; 3) The effect of treatment on apoptosis was observed by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining; 4) Western blotting was used to examine the protein expression in hippocampal neurons...
November 6, 2023: Aging
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JOURNAL ARTICLE
Xueting Liu, Xia Mao, Yudong Liu, Wenjia Chen, Weijie Li, Na Lin, Yanqiong Zhang
Tianhe Zhuifeng Gao (TZG) shows a satisfying therapeutic efficacy in treating arthromyodynia, which shares similar etiology to myofascial pain syndrome (MPS). We herein aim to explore whether TZG could be a potential prescription for MPS therapy. An MPS rat model was successfully established presenting with reduced pain thresholds, abnormal local switch responses, etc. , which was effectively reversed by TZG treatment externally. A transcriptome sequencing based on the active MTrPs samples of rats, combined with network analysis revealed that TZG might ameliorate the progression of MPS by impairing neutrophil extracellular traps (NETs) release through inhibiting PI3K-RAC2 signaling to reduce NADPH oxidase-originated ROS...
October 20, 2023: IScience
#20
JOURNAL ARTICLE
Chloe N Cordes, Melissa M Gouge, Hannah Morgan, Carah Porter, Jamie Siders, Erica Bacab, Briana Barin, Ixel A Becerra, Jose Castillo, Jared Church, Shaunacee Condo-Hicks, Kattleya J Conroy, Isbel Curbelo-Navarro, Benjamin J DePrest, Adrienne Eady, TyMahni Edmead, Verena Farouk, Angelica Flores-Torres, Michael Girmai, Cynthia I Gutierrez Navarro, Stan Guzman, Alexis B Harris, Karin Healy, Alina Jaafar, Abraham Khadr, Melissa N Kiboi, Stephanie N Korte, Celia Lopez, Hyder Mahdi, Jennifer Mendoza Avalos, Kathy Miranda, Dipti Patel, Celia Lopez, Hyder Mahdi, Jennifer Mendoza Avalos, Kathy Miranda, Dipti Patel, Rishi Patel, Sydney Pechulis, Victoria Plachta, Kamryn Rhodes, Andrea M Sandoval, Caila Thomas, Joselyn A Valadez-Mendoza, Hannah Vora, Jonathan Yousif, Kayla L Bieser, Jacob D Kagey
An EMS-based forward genetic screen was conducted in an apoptotic null background to identify genetic aberrations that contribute to regulation of cell growth in Drosophila melanogaster . The current work maps the genomic location of one of the identified mutants, L.3.2 . Genetic crosses conducted through the Fly-CURE consortium determined that the gene locus for the L.3.2 mutation is p47 on chromosome 2R.
2023: microPublication. Biology
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