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https://www.readbyqxmd.com/read/28916473/down-regulation-of-nox2-activity-in-phagocytes-mediated-by-atm-kinase-dependent-phosphorylation
#1
Sylvain Beaumel, Antoine Picciocchi, Franck Debeurme, Corinne Vives, Anne-Marie Hesse, Myriam Ferro, Didier Grunwald, Heather Stieglitz, Pahk Thepchatri, Susan M E Smith, Franck Fieschi, Marie José Stasia
NADPH oxidases (NOX) have many biological roles, but their regulation to control production of potentially toxic ROS molecules remains unclear. A previously identified insertion sequence of 21 residues (called NIS) influences NOX activity, and its predicted flexibility makes it a good candidate for providing a dynamic switch controlling the NOX active site. We constructed NOX2 chimeras in which NIS had been deleted or exchanged with those from other NOXs (NIS1, 3 and 4). All contained functional heme and were expressed normally at the plasma membrane of differentiated PLB-985 cells...
September 12, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28878675/inhibition-of-collagen-induced-platelet-aggregation-by-the-secobutanolide-secolincomolide-a-from-lindera-obtusiloba-blume
#2
Sang-Hyuk Jung, Joo-Hui Han, Hyun-Soo Park, Jung-Jin Lee, Seo Young Yang, Young Ho Kim, Kyung-Sun Heo, Chang-Seon Myung
Atherothrombosis is one of the main underlying cause of cardiovascular diseases. In addition to treating atherothrombosis with antithrombotic agents, there is growing interest in the role of natural food products and biologically active ingredients for the prevention and treatment of cardiovascular diseases. This study aimed to investigate the effect of secolincomolide A (3) isolated from Lindera obtusiloba Blume on platelet activity and identify possible signaling pathways. In our study, the antiplatelet activities of 3 were measured by collagen-induced platelet aggregation and serotonin secretion in freshly isolated rabbit platelets...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28878301/epa-dha-6-1-prevents-angiotensin-ii-induced-hypertension-and-endothelial-dysfunction-in-rats-role-of-nadph-oxidase-and-cox-derived-oxidative-stress
#3
Zahid Rasul Niazi, Grazielle C Silva, Thais Porto Ribeiro, Antonio J León-González, Mohamad Kassem, Abdur Mirajkar, Azhar Alvi, Malak Abbas, Faraj Zgheel, Valérie B Schini-Kerth, Cyril Auger
Eicosapentaenoic acid:docosahexaenoic acid (EPA:DHA) 6:1, an omega-3 polyunsaturated fatty acid formulation, has been shown to induce a sustained formation of endothelial nitric oxide (NO) synthase-derived NO, a major vasoprotective factor. This study examined whether chronic intake of EPA:DHA 6:1 prevents hypertension and endothelial dysfunction induced by angiotensin II (Ang II) in rats. Male Wister rats received orally corn oil or EPA:DHA 6:1 (500 mg kg(-1) per day) before chronic infusion of Ang II (0...
September 7, 2017: Hypertension Research: Official Journal of the Japanese Society of Hypertension
https://www.readbyqxmd.com/read/28855537/activation-of-nadph-oxidases-leads-to-dna-damage-in-esophageal-cells
#4
Vikas Bhardwaj, Ravindran Caspa Gokulan, Andela Horvat, Liudmila Yermalitskaya, Olga Korolkova, Kay M Washington, Wael El-Rifai, Sergey I Dikalov, Alexander I Zaika
Gastroesophageal reflux disease (GERD) is the strongest known risk factor for esophageal adenocarcinoma. In the center of tumorigenic events caused by GERD is repeated damage of esophageal tissues by the refluxate. In this study, we focused on a genotoxic aspect of exposure of esophageal cells to acidic bile reflux (BA/A). Analyzing cells generated from patients with Barrett's esophagus and human esophageal specimens, we found that BA/A cause significant DNA damage that is mediated by reactive-oxygen species...
August 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28835433/integration-of-trpc6-and-nadph-oxidase-activation-in-lysophosphatidylcholine-induced-trpc5-externalization
#5
Pinaki Chaudhuri, Michael A Rosenbaum, Lutz Birnbaumer, Linda M Graham
Lipid oxidation products, including lysophosphatidylcholine (lysoPC), activate TRPC6, and the subsequent increase in intracellular Ca(2+) leads to TRPC5 activation. The goal of this study is to elucidate the steps in the pathway between TRPC6 activation and TRPC5 externalization. Following TRPC6 activation by lysoPC, extracellular regulated kinase (ERK) is phosphorylated. This leads to phosphorylation of p47(phox) and subsequent NADPH oxidase activation with increased production of reactive oxygen species. ERK activation requires TRPC6 opening and influx of Ca(2+) as evidenced by the failure of lysoPC to induce ERK phosphorylation in TRPC6(-/-) endothelial cells...
August 23, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28827764/traf4-promotes-lung-cancer-aggressiveness-by-modulating-tumor-microenvironment-in-normal-fibroblasts
#6
EunGi Kim, Wanyeon Kim, Sungmin Lee, Jahyun Chun, JiHoon Kang, Gaeul Park, IkJoon Han, Hee Jung Yang, HyeSook Youn, BuHyun Youn
Normal fibroblasts surrounding tumor cells play a crucial role in cancer progression through formation of the tumor microenvironment. Because factors secreted from normal fibroblasts can modulate the tumor microenvironment, it is necessary to identify key factors associated with regulation of secreted factors and to investigate the molecular mechanisms contributing to the tumor microenvironment formation process. In this study, we found that radiation induced the expression and K63-linkage poly-ubiquitination of TRAF4 in normal lung fibroblasts...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821269/age-exacerbates-microglial-activation-oxidative-stress-inflammatory-and-nox2-gene-expression-and-delays-functional-recovery-in-a-middle-aged-rodent-model-of-spinal-cord-injury
#7
Ramona E von Leden, Guzal Khayrullina, Kasey E Moritz, Kimberly R Byrnes
BACKGROUND: Spinal cord injury (SCI) among people over age 40 has been steadily increasing since the 1980s and is associated with worsened outcome than injuries in young people. Age-related increases in reactive oxygen species (ROS) are suggested to lead to chronic inflammation. The NADPH oxidase 2 (NOX2) enzyme is expressed by microglia and is a primary source of ROS. This study aimed to determine the effect of age on inflammation, oxidative damage, NOX2 gene expression, and functional performance with and without SCI in young adult (3 months) and middle-aged (12 months) male rats...
August 18, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28793292/huangqi-decoction-ameliorates-streptozotocin-induced-rat-diabetic-nephropathy-through-antioxidant-and-regulation-of-the-tgf-%C3%AE-mapk-ppar-%C3%AE-signaling
#8
Haiyan Han, Aili Cao, Li Wang, Hengjiang Guo, Yingjun Zang, Zezheng Li, Xuemei Zhang, Wen Peng
BACKGROUND/AIMS: Huangqi Decoction (HQD) has been traditionally used to treat diabetes mellitus in China. The present study was carried out to assess the protective effect of HQD on diabetic nephropathy (DN) using the streptozotocin-induced (STZ) diabetic rats. METHODS: Diabetes was induced by a single intraperitoneal injection of STZ (60 mg/kg) in male Wistar rats. 40 diabetic rats were divided into 5 groups: vehicle-treated (DN group), 0.45, 0.15, 0.05 g/kg HQD-treated diabetic group (HQD group) and 1 mg/kg rosiglitazone-treated diabetic group (RGZ group)...
August 9, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28764528/development-of-a-high-speed-vacuum-ultraviolet-vuv-imaging-system-for-the-experimental-advanced-superconducting-tokamak
#9
Fan Zhou, Tingfeng Ming, Yumin Wang, Zhijun Wang, Feifei Long, Qing Zhuang, Guoqiang Li, Yunfeng Liang, Xiang Gao
A high-speed vacuum ultraviolet (VUV) imaging system for edge plasma studies is being developed on the Experimental Advanced Superconducting Tokamak (EAST). Its key optics is composed of an inverse type of Schwarzschild telescope made of a set of Mo/Si multilayer mirrors, a micro-channel plate (MCP) equipped with a P47 phosphor screen and a high-speed camera with CMOS sensors. In order to remove the contribution from low-energy photons, a Zr filter is installed in front of the MCP detector. With this optics, VUV photons with a wavelength of 13...
July 2017: Review of Scientific Instruments
https://www.readbyqxmd.com/read/28757373/anti-inflammatory-mechanism-of-galangin-in-lipopolysaccharide-stimulated-microglia-critical-role-of-ppar-%C3%AE-signaling-pathway
#10
Min-Ji Choi, Eun-Jung Lee, Jin-Sun Park, Su-Nam Kim, Eun-Mi Park, Hee-Sun Kim
Since microglia-associated neuroinflammation plays a pivotal role in the progression of neurodegenerative diseases, controlling microglial activation has been suggested as a potential therapeutic strategy. Here, we investigated the anti-inflammatory effects of galangin (3,5,7-trihydroxyflavone) in microglia and analyzed the underlying molecular mechanisms. Galangin inhibited the expression of inducible nitric oxide synthase (iNOS) and pro-inflammatory cytokines and enhanced the expression of anti-inflammatory interleukin (IL)-10 in lipopolysaccharide (LPS)-stimulated BV2 microglia...
July 28, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28755362/functional-assembly-of-soluble-and-membrane-recombinant-proteins-of-mammalian-nadph-oxidase-complex
#11
Hajer Souabni, Aymen Ezzine, Tania Bizouarn, Laura Baciou
Activation of phagocyte cells from an innate immune system is associated with a massive consumption of molecular oxygen to generate highly reactive oxygen species (ROS) as microbial weapons. This is achieved by a multiprotein complex, the so-called NADPH oxidase. The activity of phagocyte NADPH oxidase relies on an assembly of more than five proteins, among them the membrane heterodimer named flavocytochrome b 558 (Cytb 558), constituted by the tight association of the gp91(phox) (also named Nox2) and p22(phox) proteins...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28751937/myricitrin-modulates-nadph-oxidase-dependent-ros-production-to-inhibit-endotoxin-mediated-inflammation-by-blocking-the-jak-stat1-and-nox2-p47-phox-pathways
#12
Shimei Qi, Zunyong Feng, Qiang Li, Zhilin Qi, Yao Zhang
Myricitrin, a naturally occurring polyphenol hydroxy flavonoid, has been reported to possess anti-inflammatory properties. However, the precise molecular mechanism of myricitrin's effects on LPS-induced inflammation is unclear. In the present study, myricitrin significantly alleviated acute lung injury in mice. Myricitrin also markedly suppressed the production of NO, TNF-α, IL-6, and MCP-1 in RAW264.7 macrophage cells. The inhibition of NO was concomitant with a decrease in the protein and mRNA levels of iNOS...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28740353/anti-apoptotic-effect-of-banhasasim-tang-on-chronic-acid-reflux-esophagitis
#13
Mi-Rae Shin, Hyo-Jin An, Bu-Il Seo, Seong-Soo Roh
AIM: To evaluate the anti-apoptotic effect of banhasasim-tang (BHSST) on chronic acid reflux esophagitis (CARE) using a rat model. METHODS: A surgically-induced CARE model was established in Sprague-Dawley rats. The modeled rats were divided into a treatment group or untreated group, and given BHSST (1 g/kg body weight per day) or water, respectively, for 15 consecutive days (n = 7 each group). Changes in expression of proteins related to nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and apoptosis were assessed by western blotting...
July 7, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28733146/resveratrol-efficiently-improves-pulmonary-function-via-stabilizing-mast-cells-in-a-rat-intestinal-injury-model
#14
Xiaolei Huang, Weicheng Zhao, Dan Hu, Xue Han, Hanbin Wang, Jianyu Yang, Yang Xu, Yuantao Li, Weifeng Yao, Chaojin Chen
BACKGROUND: Intestinal ischemia/reperfusion (IIR) leads to acute lung injury (ALI) distally by aggravating pulmonary oxidative stress. Resveratrol is effective in attenuating ALI through its antioxidant capacity. This study aimed to determine the effects of resveratrol on IIR-induced ALI and to explore the role of mast cells (MCs) activation in a rat model of IIR. METHODS: Adult Sprague-Dawley rats were subjected to IIR by occluding the superior mesenteric artery for 60min followed by 4-hour reperfusion...
September 15, 2017: Life Sciences
https://www.readbyqxmd.com/read/28729672/plasmodium-berghei-p47-is-essential-for-ookinete-protection-from-the-anopheles-gambiae-complement-like-response
#15
Chiamaka Valerie Ukegbu, Maria Giorgalli, Hassan Yassine, Jose Luis Ramirez, Chrysanthi Taxiarchi, Carolina Barillas-Mury, George K Christophides, Dina Vlachou
Malaria is a mosquito-borne disease affecting millions of people every year. The rodent parasite Plasmodium berghei has served as a model for human malaria transmission studies and played a pivotal role in dissecting the mosquito immune response against infection. The 6-cysteine protein P47, known to be important for P. berghei female gamete fertility, is shown to serve a different function in Plasmodium falciparum, protecting ookinetes from the mosquito immune response. Here, we investigate the function of P...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28719308/a-recombinant-47-kda-outer-membrane-protein-induces-an-immune-response-against-orientia-tsutsugamushi-strain-boryong
#16
Sangho Choi, Hang Jin Jeong, Kyu-Jam Hwang, Byoungchul Gill, Young Ran Ju, Yeong Seon Lee, Jeongmin Lee
We investigated the 47-kDa outer membrane protein (OMP), which is a periplasmic serine protease and an antigenic major surface protein of Orientia tsutsugamushi, as a vaccine candidate. We developed a conventional subunit vaccine expressing recombinant 47-kDa OMP (rec47) and a DNA vaccine (p47). In mouse immunization experiments, intranasal immunization with rec47 alone or with rec47 plus heat-labile enterotoxin B subunit from Escherichia coli or plus cholera toxin (CT) as adjuvants induced a higher amount of rec47-specific antibodies than intramuscular immunization with p47 alone or with p47 plus pBOOST2-samIRF7/3 (pB) as adjuvant...
July 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28713487/moringa-oleifera-seeds-attenuate-vascular-oxidative-and-nitrosative-stresses-in-spontaneously-hypertensive-rats
#17
Joseph Iharinjaka Randriamboavonjy, Marc Rio, Pierre Pacaud, Gervaise Loirand, Angela Tesse
Moringa oleifera (MOI) is a tree currently used in traditional medicine in tropical Africa, America, and Asia for therapeutic applications in several disorders including arterial hypertension. We previously described a cardiac protective role of a treatment with MOI seeds in spontaneously hypertensive rats (SHR). Here, we investigated the effects of this treatment on oxidative and nitrosative vascular stresses in SHR, with normotensive Wistar Kyoto rats used as controls. Oxidative and nitrosative stresses detected in SHR aortas using the fluorescent dye dihydroethidine and protein nitrotyrosine staining were reduced in MOI-treated SHR aortas...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28659754/nadph-oxidase-isoform-2-nox2-is-involved-in-drug-addiction-vulnerability-in-progeny-developmentally-exposed-to-ethanol
#18
Marcela L Contreras, Erwin de la Fuente-Ortega, Sofía Vargas-Roberts, Daniela C Muñoz, Carolina A Goic, Paola A Haeger
Ethanol exposure increases oxidative stress in developing organs, including the brain. Antioxidant treatment during maternal ethanol ingestion improves behavioral deficits in rodent models of fetal alcohol spectrum disorder (FASD). However, the impact of general antioxidant treatment in their adult offspring and the Specific Reactive Species (ROS)-dependent mechanism, are not fully understood. We hypothesized that pre and early postnatal ethanol exposure (PEE) modifies redox homeostasis, in particular NOX2 function during reward signaling in the mesocorticolimbic pathway, which reinforces the effects of alcohol...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28638979/endocytosis-is-required-for-exocytosis-and-priming-of-respiratory-burst-activity-in-human-neutrophils
#19
T Michael Creed, Shweta Tandon, Richard A Ward, Kenneth R McLeish
OBJECTIVE AND DESIGN: Neutrophil generation of reactive oxygen species (ROS) is enhanced by exposure to pro-inflammatory agents in a process termed priming. Priming is depending on exocytosis of neutrophil granules and p47(phox) phosphorylation-dependent translocation of cytosolic NADPH oxidase components. Clathrin-mediated endocytosis was recently reported to be necessary for priming, but the mechanism linking endocytosis to priming was not identified. The present study examined the hypothesis that endocytosis regulates neutrophil priming by controlling granule exocytosis...
June 21, 2017: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/28630004/co-releasing-molecules-2-attenuates-ox-ldl-induced-injury-in-huvecs-by-ameliorating-mitochondrial-function-and-inhibiting-wnt-%C3%AE-catenin-pathway
#20
Hai-Jian Sun, Dong-Yan Xu, Yi-Xin Sun, Tong Xue, Chen-Xing Zhang, Zhi-Xuan Zhang, Wei Lin, Ke-Xue Li
Oxidized low-density lipoprotein (ox-LDL) is well known to disrupt normal functionality of endothelium, which plays a prominent role in endothelial dysfunction in many cardiovascular diseases. CO-releasing molecule 2 (CORM-2) is a promising candidate for treatment of cardiovascular diseases. However, it has not been defined whether CORM-2 might improve endothelial injury induced by ox-LDL. The present study was undertaken to determine the regulatory role of CORM-2 in cell injury of ox-LDL-treated human umbilical vein endothelial cells (HUVECs)...
August 26, 2017: Biochemical and Biophysical Research Communications
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