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https://www.readbyqxmd.com/read/28638979/endocytosis-is-required-for-exocytosis-and-priming-of-respiratory-burst-activity-in-human-neutrophils
#1
T Michael Creed, Shweta Tandon, Richard A Ward, Kenneth R McLeish
OBJECTIVE AND DESIGN: Neutrophil generation of reactive oxygen species (ROS) is enhanced by exposure to pro-inflammatory agents in a process termed priming. Priming is depending on exocytosis of neutrophil granules and p47(phox) phosphorylation-dependent translocation of cytosolic NADPH oxidase components. Clathrin-mediated endocytosis was recently reported to be necessary for priming, but the mechanism linking endocytosis to priming was not identified. The present study examined the hypothesis that endocytosis regulates neutrophil priming by controlling granule exocytosis...
June 21, 2017: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/28630004/co-releasing-molecules-2-attenuates-ox-ldl-induced-injury-in-huvecs-by-ameliorating-mitochondrial-function-and-inhibiting-wnt-%C3%AE-catenin-pathway
#2
Hai-Jian Sun, Dong-Yan Xu, Yi-Xin Sun, Tong Xue, Chen-Xing Zhang, Zhi-Xuan Zhang, Wei Lin, Ke-Xue Li
Oxidized low-density lipoprotein (ox-LDL) is well known to disrupt normal functionality of endothelium, which plays a prominent role in endothelial dysfunction in many cardiovascular diseases. CO-releasing molecule 2 (CORM-2) is a promising candidate for treatment of cardiovascular diseases. However, it has not been defined whether CORM-2 might improve endothelial injury induced by ox-LDL. The present study was undertaken to determine the regulatory role of CORM-2 in cell injury of ox-LDL-treated human umbilical vein endothelial cells (HUVECs)...
June 17, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28601846/nadph-oxidase-depletion-in-neutrophils-from-patients-with-cirrhosis-and-restoration-via-toll-like-receptor-7-8-activation
#3
Loïc Rolas, Abdelali Boussif, Emmanuel Weiss, Philippe Lettéron, Oualid Haddad, Jamel El-Benna, Pierre-Emmanuel Rautou, Richard Moreau, Axel Périanin
OBJECTIVE: Cirrhosis downregulates phagocyte oxidant production via their antibacterial superoxide-generating system, NADPH oxidase (NOX2) and increases patients' susceptibility to infection and mortality rate. To explore novel biochemical parameters that explain susceptibility to infections, we investigated the expression of NOX2 and partners in neutrophils of patients with severe alcoholic cirrhosis and have provided a novel approach to restore superoxide production capacity in patients' neutrophils and blood...
June 10, 2017: Gut
https://www.readbyqxmd.com/read/28600985/nox2-contributes-to-hyperinsulinemia-induced-redox-imbalance-and-impaired-vascular-function
#4
Abeer M Mahmoud, Mohamed M Ali, Edwin R Miranda, Jacob T Mey, Brian K Blackburn, Jacob M Haus, Shane A Phillips
Insulin resistance promotes vascular endothelial dysfunction and subsequent development of cardiovascular disease. Previously we found that skeletal muscle arteriolar flow-induced dilation (FID) was reduced following a hyperinsulinemic clamp in healthy adults. Therefore, we hypothesized that hyperinsulinemia, a hallmark of insulin resistance, contributes to microvascular endothelial cell dysfunction via inducing oxidative stress that is mediated by NADPH oxidase (Nox) system. We examined the effect of insulin, at levels that are comparable with human hyperinsulinemia on 1) FID of isolated arterioles from human skeletal muscle tissue in the presence and absence of Nox inhibitors and 2) human adipose microvascular endothelial cell (HAMECs) expression of nitric oxide (NO), endothelial NO synthase (eNOS), and Nox-mediated oxidative stress...
June 3, 2017: Redox Biology
https://www.readbyqxmd.com/read/28573047/simultaneous-fluorescein-angiography-and-spectral-domain-optical-coherence-tomography-correlate-retinal-thickness-changes-to-vascular-abnormalities-in-an-in-vivo-mouse-model-of-retinopathy-of-prematurity
#5
Olachi J Mezu-Ndubuisi, Lauren K Taylor, Jamee A Schoephoerster
BACKGROUND: Retinopathy of prematurity (ROP) is a condition of abnormal retinal vascular development (RVD) in premature infants. Fluorescein angiography (FA) has depicted phases (early, mid, late, and mature) of RVD in oxygen-induced retinopathy (OIR) mice. We sought to establish the relationship between retinal structural and vascular changes using simultaneous FA and spectral domain optical coherence tomography (SD-OCT). METHOD: 63 mice were exposed to 77% oxygen at postnatal day 7 (P7) for 5 days, while 63 mice remained in room air (RA)...
2017: Journal of Ophthalmology
https://www.readbyqxmd.com/read/28545352/cytoskeleton-anchoring-of-conformational-mutant-like-p53-but-not-shorter-isoforms-p53%C3%AE-and-p47-%C3%AE-n40p53-in-senescent-human-fibroblasts
#6
Koji Nishio
BACKGROUND: Cytoskeleton anchoring of conformational mutant-like p53 is prominent in human senescent cell. The present research investigated the structural basis of vimentin cytoskeleton-anchoring of human p53. METHOD: GFP-fused wild type p53, mutant p53, those of the various truncated isoforms including p53β and p47, were expressed in the vimentin-expressing cells: mouse fibroblasts, COS-7 cells, young and senescent human fibroblasts, and HeLa cells (non-vimentin-expressing)...
May 23, 2017: Current Aging Science
https://www.readbyqxmd.com/read/28515091/angiotensin-converting-enzyme-enhances-the-oxidative-response-and-bactericidal-activity-of-neutrophils
#7
Zakir Khan, Xiao Z Shen, Ellen A Bernstein, Jorge F Giani, Masahiro Eriguchi, Tuantuan V Zhao, Romer A Gonzalez-Villalobos, Sebastien Fuchs, George Y Liu, Kenneth E Bernstein
Angiotensin converting enzyme (ACE) inhibitors are widely used to reduce blood pressure. Here, we examined if ACE is important for the antibacterial effectiveness of neutrophils. ACE knockout mice or mice treated with an ACE inhibitor were more susceptible to bacterial infection by methicillin-resistant S. aureus (MRSA). In contrast, mice over expressing ACE in neutrophils (Neu(ACE) mice) have increased resistance to MRSA, and better in vitro killing of MRSA, P. aeruginosa, and K. pneumoniae ACE overexpression increased neutrophil production of reactive oxygen species (ROS) following MRSA challenge, an effect independent of the angiotensin II AT1 receptor...
May 17, 2017: Blood
https://www.readbyqxmd.com/read/28476935/a-schistosoma-japonicum-infection-promotes-the-expansion-of-myeloid-derived-suppressor-cells-by-activating-the-jak-stat3-pathway
#8
Quan Yang, Huaina Qiu, Hongyan Xie, Yanwei Qi, Hefei Cha, Jiale Qu, Mei Wang, Yuanfa Feng, Xin Ye, Jianbing Mu, Jun Huang
Myeloid-derived suppressor cells (MDSCs), a heterogeneous group of immune cells from the myeloid lineage, play an important part in suppression of host immune responses during many pathologic conditions, including cancer and infectious diseases. Thus, understanding the functional diversity of these cells as well as the underlying mechanisms is crucial for the development of disease control strategies. The role of MDSCs during Schistosoma japonicum infection, however, is not clear, and there is a lack of systematic study so far...
June 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28473742/nadph-oxidase-activation-contributes-to-heavy-ion-irradiation-induced-cell-death
#9
Yupei Wang, Qing Liu, Weiping Zhao, Xin Zhou, Guoying Miao, Chao Sun, Hong Zhang
Increased oxidative stress plays an important role in heavy ion radiation-induced cell death. The mechanism involved in the generation of elevated reactive oxygen species (ROS) is not fully illustrated. Here we show that NADPH oxidase activation is closely related to heavy ion radiation-induced cell death via excessive ROS generation. Cell death and cellular ROS can be greatly reduced in irradiated cancer cells with the preincubation of diphenyleneiodium, an inhibitor of NADPH oxidase. Most of the NADPH oxidase (NOX) family proteins (NOX1, NOX2, NOX3, NOX4, and NOX5) showed increased expression after heavy ion irradiation...
January 2017: Dose-response: a Publication of International Hormesis Society
https://www.readbyqxmd.com/read/28455132/garcinia-mangostana-pericarp-extract-protects-against-oxidative-stress-and-cardiovascular-remodeling-via-suppression-of-p47-phox-and-inos-in-nitric-oxide-deficient-rats
#10
Pattanapong Boonprom, Orachorn Boonla, Kanokporn Chayaburakul, Jariya Umka Welbat, Patchareewan Pannangpetch, Upa Kukongviriyapan, Veerapol Kukongviriyapan, Poungrat Pakdeechote, Parichat Prachaney
N(ω)-Nitro-l-arginine methyl ester (l-NAME)-induced hypertension and cardiovascular remodeling are associated with oxidative stress and inflammation. Garcinia mangostana Linn., has been reported to have antioxidant and anti-inflammatory properties. This study investigated whether G. mangostana pericarp extract (GME) could prevent l-NAME-induced hemodynamic alterations, cardiovascular remodeling, oxidative stress and inflammation in rats. Male Sprague-Dawley rats were given 40mg/kg/day of l-NAME in drinking water to induce hypertension, and were simultaneously treated with GME at a dose of 200mg/kg/day...
April 26, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28438648/the-superoxide-dismutase-mimetic-tempol-blunts-diabetes-induced-upregulation-of-nadph-oxidase-and-endoplasmic-reticulum-stress-in-a-rat-model-of-diabetic-nephropathy
#11
Miles J De Blasio, Anand Ramalingam, Anh H Cao, Darnel Prakoso, Ji-Ming Ye, Raelene Pickering, Anna M D Watson, Judy B de Haan, David M Kaye, Rebecca H Ritchie
Endoplasmic reticulum (ER) stress contributes to progression of diabetic nephropathy, which promotes end-stage renal failure in diabetic patients. This study was undertaken to investigate the actions of tempol and ramipril, pharmacological agents that target the consequences of NADPH oxidase, on diabetic nephropathy in a rat model of type 1 diabetes, with an emphasis on markers of ER stress. Male Sprague-Dawley rats were injected intravenously with a single bolus of streptozotocin (55mg/kg) to induce type 1 diabetes...
July 15, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28413457/persicarin-isolated-from-oenanthe-javanica-protects-against-diabetes-induced-oxidative-stress-and-inflammation-in-the-liver-of-streptozotocin-induced-type-1-diabetic-mice
#12
Joo Young Lee, Min Yeong Kim, Sung Ho Shin, Mi-Rae Shin, O Jun Kwon, Tae Hoon Kim, Chan Hum Park, Jeong Sook Noh, Man Hee Rhee, Seong-Soo Roh
Persicarin is one of the major components of the Oenanthe javanica (water dropwort). The present study was aimed to evaluate the role of persicarin in the hepatic tissue of streptozotocin-induced type 1 diabetic mice. Diabetes was induced by single intra-peritoneal injection of streptozotocin (120 mg/kg body weight) and then oral administration of persicarin at a dose 2.5 and 5 mg/kg body weight for 10 days. Serum and hepatic glucose levels were increased in diabetic control mice, while persicarin treatment groups were markedly reduced...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28408971/long-term-effects-of-maternal-deprivation-on-redox-regulation-in-rat-brain-involvement-of-nadph-oxidase
#13
Branka Marković, Nevena V Radonjić, Gordana Jevtić, Tihomir Stojković, Milica Velimirović, Milan Aksić, Joko Poleksić, Tatjana Nikolić, Dubravka Aleksić, Vidosava Radonjić, Branislav Filipović, Nataša D Petronijević
Maternal deprivation (MD) causes perinatal stress, with subsequent behavioral changes which resemble the symptoms of schizophrenia. The NADPH oxidase is one of the major generators of reactive oxygen species, known to play a role in stress response in different tissues. The aim of this study was to elucidate the long-term effects of MD on the expression of NADPH oxidase subunits (gp91(phox), p22(phox), p67(phox), p47(phox), and p40(phox)). Activities of cytochrome C oxidase and respiratory chain Complex I, as well as the oxidative stress parameters using appropriate spectrophotometric techniques were analyzed...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28356734/therapeutic-effects-of-proteoliposomes-on-x-linked-chronic-granulomatous-disease-proof-of-concept-using-macrophages-differentiated-from-patient-specific-induced-pluripotent-stem-cells
#14
Julie Brault, Guillaume Vaganay, Aline Le Roy, Jean-Luc Lenormand, Sandra Cortes, Marie José Stasia
Chronic granulomatous disease (CGD) is a rare inherited immunodeficiency due to dysfunction of the phagocytic nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex leading to severe and recurrent infections in early childhood. The main genetic form is the X-linked CGD leading to the absence of cytochrome b558 composed of NOX2 and p22 (phox) , the membrane partners of the NADPH oxidase complex. The first cause of death of CGD patients is pulmonary infections. Recombinant proteoliposome-based therapy is an emerging and innovative approach for membrane protein delivery, which could be an alternative local, targeted treatment to fight lung infections in CGD patients...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28351780/cordycepin-negatively-modulates-lipopolysaccharide-induced-cytokine-production-by-up-regulation-of-heme-oxygenase-1
#15
Rui Qing, Zezhi Huang, Yufei Tang, Qingke Xiang, Fan Yang
AIMS: The present study is to investigate the effect of cordycepin on the expression of heme oxygenase-1 (HO-1) in lipopolysaccharide (LPS)-activated microphages, as well as its mechanism of action. METHODS: Mouse RAW264.7 cells were treated with different concentrations of cordycepin for 0-16h. Western blotting was used to determine the expression of HO-1 and the phosphorylation of c-Src and the p47(phox) subunit of NADPH oxidase. Intracellular reactive oxygen species (ROS) level was determined using H2DCFDA as fluorescent probe...
March 25, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28331194/post-treatment-with-ma-huang-tang-ameliorates-cold-warm-cycles-induced-rat-lung-injury
#16
Meng-Meng Xiao, Chun-Shui Pan, Yu-Ying Liu, Li-Qian Ma, Li Yan, Jing-Yu Fan, Chuan-She Wang, Rong Huang, Jing-Yan Han
Frequent and drastic ambient temperature variation may cause respiratory diseases such as common cold and pneumonia, the mechanism for which is not fully understood, however, due to lack of appropriate animal models. Ma-Huang-Tang (MHT) is widely used in China for treatment of respiratory diseases. The present study aimed to investigate the effect of MHT on temperature alternation induced rat lung injury and explore underlying mechanisms. Male Sprague-Dawley rats were exposed to a cold environment for 1 h and then shifted to a warm environment for 30 min...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28322021/cytoprotective-and-antioxidant-effects-of-steen-solution-on-human-lung-spheroids-and-human-endothelial-cells
#17
F Pagano, C Nocella, S Sciarretta, L Fianchini, C Siciliano, G Mangino, M Ibrahim, E De Falco, R Carnevale, I Chimenti, G Frati
Respiratory diseases represent a major healthcare burden worldwide. Lung transplantation (LTx) is the "gold standard" for end-stage patients, strongly limited by shortage of available/suitable donor lungs. Normothermic ex vivo lung perfusion (EVLP) has significantly increased the number of lungs suitable for transplantation. Steen solution is used for EVLP, but the mechanisms involved in its beneficial properties remain to be clarified. We investigated the effects of Steen solution in an in vitro protocol of cold starvation and normothermic recovery on human lung spheroids, named pneumospheres (PSs), containing epithelial/basal cells, and on endothelial human umbilical vein endothelial cells (HUVEC)...
March 20, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28296340/lactadherin-is-a-candidate-oviduct-lewis-x-trisaccharide-receptor-on-porcine-spermatozoa
#18
E Silva, D Frost, L Li, N Bovin, D J Miller
A recent study has demonstrated that porcine spermatozoa recognize with high affinity carbohydrate structures containing Lewis X motifs. Sperm adhesion to Lewis X is proposed to mediate sperm binding to the oviduct epithelium to form a reservoir. The objective of this study was to identify Lewis X-binding proteins from porcine spermatozoa as candidate receptors for oviduct glycans. To identify low-abundance proteins typically masked by proteins originating from seminal fluid, Lewis X candidate receptors were enriched from cauda epididymal boar spermatozoa...
May 2017: Andrology
https://www.readbyqxmd.com/read/28292711/co-releasing-molecules-corm2-attenuates-angiotensin-ii-induced-human-aortic-smooth-muscle-cell-migration-through-inhibition-of-ros-il-6-generation-and-matrix-metalloproteinases-9-expression
#19
Ming-Horng Tsai, Chiang-Wen Lee, Lee-Fen Hsu, Shu-Yu Li, Yao-Chang Chiang, Ming-Hsueh Lee, Chun-Han Chen, Hwey-Fang Liang, Jia-Mei How, Pey-Jium Chang, Ching-Mei Wu, I-Ta Lee
Ang II has been involved in the pathogenesis of cardiovascular diseases, and matrix metalloproteinase-9 (MMP-9) induced migration of human aortic smooth muscle cells (HASMCs) is the most common and basic pathological feature. Carbon monoxide (CO), a byproduct of heme breakdown by heme oxygenase, exerts anti-inflammatory effects in various tissues and organ systems. In the present study, we aimed to investigate the effects and underlying mechanisms of carbon monoxide releasing molecule-2 (CORM-2) on Ang II-induced MMP-9 expression and cell migration of HASMCs...
August 2017: Redox Biology
https://www.readbyqxmd.com/read/28287132/crispr-cas9-generated-p47-phox-deficient-cell-line-for-chronic-granulomatous-disease-gene-therapy-vector-development
#20
Dominik Wrona, Ulrich Siler, Janine Reichenbach
Development of gene therapy vectors requires cellular models reflecting the genetic background of a disease thus allowing for robust preclinical vector testing. For human p47(phox)-deficient chronic granulomatous disease (CGD) vector testing we generated a cellular model using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 to introduce a GT-dinucleotide deletion (ΔGT) mutation in p47(phox) encoding NCF1 gene in the human acute myeloid leukemia PLB-985 cell line. CGD is a group of hereditary immunodeficiencies characterized by impaired respiratory burst activity in phagocytes due to a defective phagocytic nicotinamide adenine dinucleotide phosphate (NADPH) oxidase...
March 13, 2017: Scientific Reports
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