keyword
MENU ▼
Read by QxMD icon Read
search

candid

keyword
https://www.readbyqxmd.com/read/28823128/molecular-cloning-and-expression-analysis-of-annexin-a2-gene-in-sika-deer-antler-tip
#1
Yanling Xia, Haomiao Qu, Binshan Lu, Qiang Zhang, Heping Li
Objective: Molecular cloning and bioinformatics analysis of Annexin A2 gene in sika deer antler tip were conducted. The role of Annexin A2 gene in the growth and development of the antler were analyzed initially. Methods: The reverse transcriptase-polymerase chain reaction (RT-PCR) was used to clone the cDNA sequence of the Anxa2 gene from antler tip of sika deer (Cervus nippon hortulorum) and the bioinformatics tools provided on the web site were applied to analyze the amino acid sequence of Anxa2 protein...
August 16, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28823039/hydroponic-screening-of-fast-growing-tree-species-for-lead-phytoremediation-potential
#2
Jiraporn Yongpisanphop, Sandhya Babel, Maleeya Kruatrachue, Prayad Pokethitiyook
Using trees as phytoremediators has become a powerful tool to remediate lead from contaminated environments. This study aims to identify potential candidates among fast-growing trees by comparing their ability to tolerate and accumulate Pb. Cuttings from Acacia mangium, Azadirachta indica, Eucalyptus camaldulensis, and Senna siamea were cultured in 25% modified Hoagland's solutions supplemented with 10, 30, and 50 mg/L Pb for 15 days. Lead concentrations were determined by a flame atomic absorption spectrophotometer...
August 20, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28823010/the-reliability-of-rifampicin-resistance-as-a-proxy-for-multidrug-resistant-tuberculosis-a-systematic-review-of-studies-from-iran
#3
REVIEW
M J Nasiri, S Zamani, A Pormohammad, M M Feizabadi, H R Aslani, M Amin, R Halabian, A A Imani Fooladi
In Iran, patients showing rifampicin (RIF) resistance detected by the Xpert® MTB/RIF assay are considered as candidates for multidrug-resistant tuberculosis (MDR-TB) treatment. Despite the fact that RIF resistance has been used as a proxy for MDR-TB, little is known about the proportion of isoniazid (INH) resistance patterns in RIF-resistant TB. We systematically searched MEDLINE, Embase, and other databases up to March 2017 for studies addressing the proportion of INH resistance patterns in RIF-resistant TB in Iran...
August 19, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28822867/de-novo-assembly-and-characterization-of-seabass-lateolabrax-japonicus-transcriptome-and-expression-of-hepatic-genes-following-different-dietary-phosphorus-calcium-levels
#4
Kang-Le Lu, Zhong-Li Ji, Samad Rahimnejad, Chun-Xiao Zhang, Ling Wang, Kai Song
Fish farming seriously influences the aquatic environment because most dietary phosphorus (P) is excreted in the effluent. To increase the P utilization in fish, molecular techniques should be explored given the remarkable development of these techniques. Thus, to identify the candidate genes related to P utilization and molecular alterations following administration of a P-deficient diet in seabass Lateolabrax japonicus, we assessed the de novo pituitary, gill, intestine, liver, kidney, scales and vertebra transcriptomes, and we compared the expression of hepatic genes with three diets varying in P and Ca levels: diet I (0...
August 9, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/28822863/an-x-ray-absorption-fine-structure-spectroscopy-study-of-metal-sorption-to-graphene-oxide
#5
Allison R Showalter, Thomas A Duster, Jennifer E S Szymanowski, Chongzheng Na, Jeremy B Fein, Bruce A Bunker
Remediation and prevention of environmental contamination by toxic metals is an ongoing issue. Additionally, improving water filtration systems is necessary to prevent toxic metals from circulating through the water supply. Graphene oxide (GO) is a highly sorptive material for a variety of heavy metals under different ionic strength conditions over a wide pH range, making it a promising candidate for use in metal adsorption from contaminated sites or in filtration systems. We present X-ray absorption fine structure (XAFS) spectroscopy results investigating the binding environment of Cd (II), U(VI) and Pb(II) ions onto multi-layered graphene oxide (MLGO)...
August 14, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28822859/a-facile-method-to-prepare-green-nano-phosphors-with-a-large-stokes-shift-and-solid-state-enhanced-photophysical-properties-based-on-surface-modified-gold-nanoclusters
#6
C H Cheng, H Y Huang, M J Talite, W C Chou, J M Yeh, C T Yuan
Colloidal nano-materials, such as quantum dots (QDs) have been applied to light-conversion nano-phosphors due to their unique tunable emission. However, most of the QDs involve toxic elements and are synthesized in a hazardous solvent. In addition, conventional QD nano-phosphors with a small Stokes shift suffered from reabsorption losses and aggregation-induced quenching in the solid state. Here, we demonstrate a facile, matrix-free method to prepare eco-friendly nano-phosphors with a large Stokes shift based on aqueous thiolate-stabilized gold nanoclusters (GSH-AuNCs) with simple surface modifications...
August 8, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28822800/amelogenin-induces-m2-macrophage-polarisation-via-pge2-camp-signalling-pathway
#7
Kensuke Yamamichi, Takao Fukuda, Terukazu Sanui, Kyosuke Toyoda, Urara Tanaka, Yuki Nakao, Karen Yotsumoto, Hiroaki Yamato, Takaharu Taketomi, Takeshi Uchiumi, Fusanori Nishimura
OBJECTIVES: Amelogenin, the major component of the enamel matrix derivative (EMD), has been suggested as a bioactive candidate for periodontal regeneration. Apart from producing a regenerative effect on periodontal tissues, amelogenin has also been reported to have an anti-inflammatory effect. However, the precise molecular mechanisms underlying these effects remain unclear. In the present study, we examined the immunomodulatory effects of amelogenin on macrophages. DESIGN: Human phorbol 12-myristate 13-acetate (PMA)-differentiated U937 macrophages and CD14+ peripheral blood-derived monocytes (PBMC)-derived macrophages were stimulated with recombinant amelogenin (rM180)...
August 12, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28822783/the-expression-induction-and-pharmacological-activity-of-cyp1a2-are-post-transcriptionally-regulated-by-microrna-hsa-mir-132-5p
#8
Yinting Chen, Linjuan Zeng, Yong Wang, William H Tolleson, Bridgett Knox, Si Chen, Zhen Ren, Lei Guo, Nan Mei, Feng Qian, Kaihong Huang, David Liu, Weida Tong, Dianke Yu, Baitang Ning
Cytochrome P450 1A2 (CYP1A2) is one of the most abundant and important drug metabolizing enzymes in human liver. However, little is known about the post-transcriptional regulation of CYP1A2, especially the mechanisms involving microRNAs (miRNAs). This study applied a systematic approach to investigate the post-transcriptional regulation of CYP1A2 by miRNAs. Candidate miRNAs targeting the 3'-untranslated region (3'-UTR) of CYP1A2 were screened in silico, resulting in the selection of sixty-two potential miRNAs for further analysis...
August 16, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28822774/effects-of-neuropeptides-and-sex-steroids-on-the-pituitary-gonadal-axis-of-pre-pubertal-f1-wreckfish-h%C3%A4-puku-polyprion-oxygeneios-in-vivo-evidence-of-inhibitory-effects-of-androgens
#9
Matthew J Wylie, Alvin N Setiawan, Glen W Irvine, Jane E Symonds, Abigail Elizur, P Mark Lokman
The ability to advance puberty in broodstock that have a long generation interval and mature at large size is a highly valuable tool in contemporary aquaculture enterprise. Juvenile male and female wreckfish 'hāpuku' (Polyprion oxygeneios), a candidate for commercialization in aquaculture, were subjected to treatment for 8 weeks with two implants, one containing steroid (blank; estradiol-17β, E2; 11-ketotestosterone, KT; 17 α -methyltestosterone, MT), the other peptide (blank; gonadotropin-releasing hormone analog, GnRHa; kisspeptin, Kiss2-12)...
August 16, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28822700/full-length-genome-of-a-novel-genotype-3-hepatitis-e-virus-strain-obtained-from-domestic-pigs-in-japan
#10
Putu Prathiwi Primadharsini, Masao Miyake, Satoshi Kunita, Tsutomu Nishizawa, Masaharu Takahashi, Shigeo Nagashima, Tanggis, Hiroshi Ohnishi, Tominari Kobayashi, Takashi Nishiyama, Suljid Jirintai, Hiroaki Okamoto
Hepatitis E virus (HEV) causes acute or chronic hepatitis in humans and can be transmitted via the fecal-oral route. Pigs are one of the main reservoirs for this infection. Sixty pigs, 4-5 months of age, on a swine herd in Japan had detectable anti-HEV IgG antibodies, and five (8.3%) of them had ongoing infection of genotype 3 HEV. Five HEV strains obtained from the viremic pigs shared 98.8-100% nucleotide identity, and one representative strain (swHE1606845), whose entire genomic sequence was determined in this study, differed by 14...
August 16, 2017: Virus Research
https://www.readbyqxmd.com/read/28822691/fatal-meningitis-in-swine-after-intrathecal-administration-of-adeno-associated-virus-expressing-syngeneic-interleukin-10
#11
Mark D Unger, Josef Pleticha, James E Collins, Anibal G Armien, Jennifer L Brazzell, Laura K Newman, Lukas F Heilmann, Jodi A Scholz, Timothy P Maus, Andreas S Beutler
Interleukin-10 (IL-10) delivered by intrathecal (i.t.) gene vectors is a candidate investigational new drug (IND) for several chronic neurological disorders such as neuropathic pain. We performed a preclinical safety study of IL-10. A syngeneic large animal model was used delivering porcine IL-10 (pIL-10) to the i.t. space in swine by adeno-associated virus serotype 8 (AAV8), a gene vector that was previously found to be nontoxic in the i.t. space. Unexpectedly, animals became ill, developing ataxia, seizures, and an inability to feed and drink, and required euthanasia...
August 1, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28822631/clinical-evaluation-of-ebola-virus-disease-therapeutics
#12
REVIEW
Guodong Liu, Gary Wong, Shuo Su, Yuhai Bi, Frank Plummer, George F Gao, Gary Kobinger, Xiangguo Qiu
Ebola virus disease (EVD) was first described over 40 years ago, but no treatment has been approved for humans. The 2013-2016 EVD outbreak in West Africa has expedited the clinical evaluation of several candidate therapeutics that act through different mechanisms, but with mixed results. Nevertheless, these studies are important because the accumulation of clinical data and valuable experience in conducting efficacy trials under emergency circumstances will lead to better implementation of similar studies in the future...
August 16, 2017: Trends in Molecular Medicine
https://www.readbyqxmd.com/read/28822624/evaluating-an-australian-emergency-nurse-practitioner-candidate-training-program
#13
Sharyn J Plath, Mary Wright, Julia Hocking
Nurse Practitioners (NPs) receive core clinical training at master's level, with their employer providing the opportunity to upskill in clinical and procedural competencies. It is increasingly recognised that this generic education requires supplementary training for operating effectively within a specific clinical environment. In this paper we describe a pilot program designed to train Australian NP Candidates to work effectively within the Emergency Department Fast Track model of care. The training program consisted of a 12-month period: four hours in-house training per week over two semesters, running concurrently with the NP candidate's University semesters, and 3 months' clinical practice to consolidate...
August 16, 2017: Australasian Emergency Nursing Journal: AENJ
https://www.readbyqxmd.com/read/28822551/disruption-of-staphylococcus-aureus-biofilms-using-rhamnolipid-biosurfactants
#14
S S E Silva, J W P Carvalho, C P Aires, M Nitschke
Staphylococcus aureus is an important pathogen that has shown ability to establish biofilm communities that can represent a source of contamination and resistance in food processing. Rhamnolipids (RL) have attracted attention as candidates to replace synthetic surfactants, exhibiting high surface activity combined with its microbial origin, biodegradability, and low toxicity. In this work, an RL biosurfactant was evaluated regarding its ability to disrupt or remove S. aureus biofilms established on polystyrene plates using nutrient broth and skim milk as the growth media...
August 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28822148/-dna-barcoding-identification-of-endangered-medicinal-plants-of-orchidaceae
#15
Huan Tang, Li Xiang, Xi-Wen Li, Wei Sun, Mei-Na Wang, Yun-Feng Huang, Meng Ye
In this study, DNA barcoding was used to validate the traditional morphological classification of medicinal plants of Orchidaceae. The 163 samples of 135 species belong to 49 genera which have been confirmed by morphological identification were collected. Candidate sequences, including matK, psbA-trnH and ITS2 sequences, were amplified, bidirectionally sequenced, and assembled. All the sequences were blasted to GenBank database at NCBI, then analyzed using Neighbor-joining tree method by MEGA 7.0. The results showed that the DNAs of 163 samples were successfully extracted...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822130/exploiting-natural-variation-to-discover-candidate-genes-involved-in-photosynthesis-related-traits
#16
Franklin Magnum de Oliveira Silva, Lucas de Ávila Silva, Wagner L Araújo, Agustin Zsögön, Adriano Nunes-Nesi
Naturally occurring genetic variation in plants can be very useful to dissect the complex regulation of primary metabolism as well as of physiological traits such as photosynthesis and photorespiration. The physiological and genetic mechanisms underlying natural variation in closely related species or accessions may provide important information that can be used to improve crop yield. In this chapter we describe in detail the use of a population of introgression lines (ILs), with the Solanum pennellii IL population as a study case, as a tool for the identification of genomic regions involved in the control of photosynthetic efficiency...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28822116/interactions-between-variation-in-candidate-genes-and-environmental-factors-in-the-etiology-of-schizophrenia-and-bipolar-disorder-a-systematic-review
#17
REVIEW
Błażej Misiak, Filip Stramecki, Łukasz Gawęda, Katarzyna Prochwicz, Maria M Sąsiadek, Ahmed A Moustafa, Dorota Frydecka
Schizophrenia and bipolar disorder (BD) are complex and multidimensional disorders with high heritability rates. The contribution of genetic factors to the etiology of these disorders is increasingly being recognized as the action of multiple risk variants with small effect sizes, which might explain only a minor part of susceptibility. On the other site, numerous environmental factors have been found to play an important role in their causality. Therefore, in recent years, several studies focused on gene × environment interactions that are believed to bridge the gap between genetic underpinnings and environmental insults...
August 18, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28822103/current-progress-in-innovative-engineered-antibodies
#18
REVIEW
William R Strohl
As of May 1, 2017, 74 antibody-based molecules have been approved by a regulatory authority in a major market. Additionally, there are 70 and 575 antibody-based molecules in phase III and phase I/II clinical trials, respectively. These total 719 antibody-based clinical stage molecules include 493 naked IgGs, 87 antibody-drug conjugates, 61 bispecific antibodies, 37 total Fc fusion proteins, 17 radioimmunoglobulins, 13 antibody fragments, and 11 immunocytokines. New uses for these antibodies are being discovered each year...
August 18, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28822073/isorhynchophylline-attenuates-mpp-induced-apoptosis-through-endoplasmic-reticulum-stress-and-mitochondria-dependent-pathways-in-pc12-cells-involvement-of-antioxidant-activity
#19
Xiao-Ming Li, Xiao-Jie Zhang, Miao-Xian Dong
Endoplasmic reticulum stress (ERS) and mitochondrial dysfunctions are thought to be involved in the dopaminergic neuronal death in Parkinson's disease (PD). In this study, we found that isorhynchophylline (IRN) significantly attenuated 1-methyl-4-phenylpyridinium (MPP(+))-induced apoptotic cell death and oxidative stress in PC12 cells. IRN markedly reduced MPP(+)-induced-ERS responses, indicative of inositol-requiring enzyme 1 (IRE1) phosphorylation and caspase-12 activation. Furthermore, IRN inhibits MPP(+)-triggered apoptosis signal-regulating kinase 1 (ASK1)/c-Jun N-terminal Kinase (JNK) signaling-mediated mitochondria-dependent apoptosis pathway...
August 18, 2017: Neuromolecular Medicine
https://www.readbyqxmd.com/read/28822019/gypenoside-ix-suppresses-p38-mapk-akt-nf%C3%AE%C2%BAb-signaling-pathway-activation-and-inflammatory-responses-in-astrocytes-stimulated-by-proinflammatory-mediators
#20
Xiaoshuang Wang, Liu Yang, Li Yang, Faping Xing, Hua Yang, Liyue Qin, Yunyi Lan, Hui Wu, Beibei Zhang, Hailian Shi, Cheng Lu, Fei Huang, Xiaojun Wu, Zhengtao Wang
Gypenoside IX (GP IX) is a pure compound isolated from Panax notoginseng. Gypenosides have been implicated to benefit the recovery of enormous neurological disorders. By suppressing the activation of astrocytes, gypenosides can improve the cognitive impairment. However, so far, little is known about whether GP IX could restrain the inflammatory responses in astrocytes or reactive astrogliosis. In present study, the anti-inflammatory effects of GP IX were investigated in reactive astrocytes induced by proinflammatory mediators both in vitro and in vivo...
August 18, 2017: Inflammation
keyword
keyword
86771
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"