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https://www.readbyqxmd.com/read/29045939/very-low-dose-decitabine-is-effective-in-treating-intermediate-or-high-risk-myelodysplastic-syndrome
#1
Hongmin Li, Liru Wang, Yue Wu, Li Su, Hong Zhao, Yongqing Zhang, Zhao Wang, Dayong Huang, Zhongxia Huang, Xiaoxiong Wu, Xiaohong Li, Fang Ye, Fan Yu, Hui Liu, Jing-Wen Wang, Jia Cong, Wanjun Sun, Hui-Ren Chen, Jingbo Wang, Bing Han
Nowadays, the regular recommended dose of decitabine for the treatment of myelodysplastic syndrome (MDS) is 20 mg/m2/day for 5 consecutive days with a relatively high incidence of treatment-related morbidities and costs. In this study, a retrospective and multicenter analysis was performed to explore the very-low-dose decitabine schedule for the treatment of patients with IPSS intermediate- or high-risk MDS. A total of 31 newly diagnosed MDS cases from 14 hospitals in Beijing received decitabine monotherapy (decitabine 6 mg/m2/day intravenously for 7 consecutive days, repeated every 4 weeks)...
October 19, 2017: Acta Haematologica
https://www.readbyqxmd.com/read/29045926/enhanced-enzymatic-saccharification-of-sugarcane-bagasse-pretreated-by-sodium-methoxide-with-glycerol
#2
Xiaojing Lv, Jianghai Lin, Liang Luo, Dou Zhang, Senlin Lei, Wenjuan Xiao, Yuan Xu, Yingxue Gong, Zehuan Liu
Sodium methoxide (CH3ONa) with glycerol pretreatment (CWGP) was performed to improve the enzymatic digestibility of sugarcane bagasse (SCB). Response surface methodology was utilized to optimize the CWGP parameters for pretreating SCB from the perspective of total fermentable sugar yield (TFSY) and total fermentable sugar concentration (TFSC). Under the optimal CWGP conditions, 0.5666g/g of TFSY (0.82% CH3ONa, 1.11h, 150°C) and 17.75g/L of TFSC (0.87% CH3ONa, 1.38h, 149.27°C) were achieved, corresponding to delignification of 79...
September 22, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29045910/immunogenicity-and-protective-efficacy-of-multistage-vaccine-candidates-mtb8-4-hspx-and-hspx-mtb8-4-against-mycobacterium-tuberculosis-infection-in-mice
#3
Wanbo Liu, Jingjing Li, Hongxia Niu, Xiaofa Lin, Ruiying Li, Yue Wang, Qi Xin, Hongjuan Yu, Yumin Wu, Bingdong Zhu, Jiying Tan
In this study, Mtb8.4 and HspX, which are expressed at proliferating and dormant stages of Mycobacterium tuberculosis (M. tuberculosis), respectively, were chosen to construct two fusion proteins, Mtb8.4-HspX (8.4H) and HspX-Mtb8.4 (H8.4), and we investigated whether the antigen dose and protein sequential order could impact the immunogenicity and protective efficacy of these fusion protein vaccines against M. tuberculosis. C57BL/6 mice were vaccinated with new constructions containing a fusion protein with adjuvant of N, N'-dimethyl-N, N'-dioctadecylammonium bromide (DDA) or a mixed adjuvant composed of DDA, polyribocytidylic acid and gelatin (DPG), and the antigen specific immune responses and protective efficacy against M...
October 15, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/29045883/in-vitro-in-vivo-correlations-of-carbamazepine-nano-dispersions-for-application-in-formulation-development
#4
Zachary Warnken, Michael Puppolo, Justin Hughey, Iris Duarte, Susan Jansen-Varnum
During formulation development, efficiently integrating in vitro dissolution testing can significantly improve one's ability to estimate in vivo performance and aide in the selection of premier drug candidates. The concept of in vitro - in vivo relationship/correlation (IVIVR/IVIVC) has garnered significant attention from pharmaceutical scientists to predict expected bioavailability characteristics for drug substances and products. The current work illustrates a comparative evaluation of in vitro tests to access crystalline carbamazepine and various types of amorphous and crystalline dispersions of carbamazepine and Eudragit L100 produced by spray-drying, including a membrane-permeation dissolution methodology and non-sink dissolution...
October 15, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29045858/circulating-circular-rna-hsa_circ_0001785-acts-as-a-diagnostic-biomarker-for-breast-cancer-detection
#5
Wei-Bing Yin, Ming-Guang Yan, Xiao Fang, Jian-Jun Guo, Ruo-Peng Zhang, Wei Xiong
BACKGROUND: Circular RNAs (circRNAs) are a class of non-coding RNAs (ncRNAs) and characterized by covalently closed loop without 5' and 3' end. Recently, the diagnostic value of circRNAs has received more and more attention. However, the in-depth study about circRNAs diagnosis in breast cancer is still rarely reported. In present study, we try to investigate the circRNAs expression profiles in breast cancer peripheral blood and discover valuable diagnostic biomarkers. METHODS: The expression profiles of circRNAs in plasma specimens were screened from five breast cancer and paired healthy volunteer s...
October 15, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29045845/revealing-the-determinants-of-widespread-alternative-splicing-perturbation-in-cancer
#6
Yongsheng Li, Nidhi Sahni, Rita Pancsa, Daniel J McGrail, Juan Xu, Xu Hua, Jasmin Coulombe-Huntington, Michael Ryan, Boranai Tychhon, Dhanistha Sudhakar, Limei Hu, Michael Tyers, Xiaoqian Jiang, Shiaw-Yih Lin, M Madan Babu, Song Yi
It is increasingly appreciated that alternative splicing plays a key role in generating functional specificity and diversity in cancer. However, the mechanisms by which cancer mutations perturb splicing remain unknown. Here, we developed a network-based strategy, DrAS-Net, to investigate more than 2.5 million variants across cancer types and link somatic mutations with cancer-specific splicing events. We identified more than 40,000 driver variant candidates and their 80,000 putative splicing targets deregulated in 33 cancer types and inferred their functional impact...
October 17, 2017: Cell Reports
https://www.readbyqxmd.com/read/29045803/chitosan-based-trilayer-scaffold-for-multitissue-periodontal-regeneration
#7
E M Varoni, S Vijayakumar, E Canciani, A Cochis, L De Nardo, G Lodi, L Rimondini, M Cerruti
Periodontal regeneration is still a challenge for periodontists and tissue engineers, as it requires the simultaneous restoration of different tissues-namely, cementum, gingiva, bone, and periodontal ligament (PDL). Here, we synthetized a chitosan (CH)-based trilayer porous scaffold to achieve periodontal regeneration driven by multitissue simultaneous healing. We produced 2 porous compartments for bone and gingiva regeneration by cross-linking with genipin either medium molecular weight (MMW) or low molecular weight (LMW) CH and freeze-drying the resulting scaffolds...
October 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/29045781/development-of-clickable-monophosphoryl-lipid-as-towards-semi-synthetic-conjugates-with-tumor-associated-carbohydrate-antigens
#8
Marcello Ziaco, Sabina Górska, Serena Traboni, Agnieszka Maria Razim, Angela Casillo, Alfonso Iadonisi, Andrzej Gamian, Maria Michela Corsaro, Emiliano Bedini
A semi-synthetic strategy for the obtainment of monophosphoryl lipid A derivatives equipped with clickable (azide, alkyne, double bond, a thiol precursor) moieties, starting from the native lipid A isolated from Escherichia coli, is here presented. These lipid A derivatives can be conjugated with other interesting biomolecules, such as tumor-associated carbohydrate antigens (TACAs). In this way, the immunostimulant activity of monophosphoryl lipid A can significantly improve the immunogenicity of TACAs, thus opening an access to potential self-adjuvant anticancer vaccine candidates...
October 18, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29045754/genomic-studies-of-local-adaptation-in-natural-plant-populations
#9
Victoria L Sork
Local adaptation arises as a result of selection by the local environment favoring phenotypes that enhance fitness. Geographic patterns of phenotypic variation are in part due to this selective process. Classically, the genetic basis of those phenotypes has been studied in plant populations using a quantitative genetic approach in which plants from different source populations are grown in common environments, in reciprocal transplant experiments, or in studies across a wide geographic and environmentally heterogeneous area...
October 14, 2017: Journal of Heredity
https://www.readbyqxmd.com/read/29045729/knockout-mice-for-dyslexia-susceptibility-gene-homologs-kiaa0319-and-kiaa0319l-have-unaffected-neuronal-migration-but-display-abnormal-auditory-processing
#10
Luiz G Guidi, Jane Mattley, Isabel Martinez-Garay, Anthony P Monaco, Jennifer F Linden, Antonio Velayos-Baeza, Zoltán Molnár
Developmental dyslexia is a neurodevelopmental disorder that affects reading ability caused by genetic and non-genetic factors. Amongst the susceptibility genes identified to date, KIAA0319 is a prime candidate. RNA-interference experiments in rats suggested its involvement in cortical migration but we could not confirm these findings in Kiaa0319-mutant mice. Given its homologous gene Kiaa0319L (AU040320) has also been proposed to play a role in neuronal migration, we interrogated whether absence of AU040320 alone or together with KIAA0319 affects migration in the developing brain...
October 17, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/29045689/sensitivity-to-house-dust-mites-allergens-with-atopic-asthma-and-its-relationship-with-cd14-c-159t-polymorphism-in-patients-of-west-bengal-india
#11
Amlan Ghosh, Shampa Dutta, Sanjoy Podder, Priti Mondal, Arghya Laha, Nimai Chandra Saha, Saibal Moitra, Goutam Kumar Saha, Richard Johnson
India is the home to around 15-20 million asthmatics, and asthma prevalence is increasing in Indian metropolitan area, including Kolkata, West Bengal. Complex interactions of genetic and environmental factors are involved in asthma. Genome-wide search for susceptible loci regulating IgE response (atopy) have identified a candidate gene CD14 which is most important in the context of allergic responses of respiratory system. This study was aimed to investigate the role of house dust and house dust mites in development of bronchial asthma and to explore the possible association of candidate gene CD14 with disease manifestation among Kolkata patient population...
October 17, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29045613/genome-structure-of-rosa-multiflora-a-wild-ancestor-of-cultivated-roses
#12
Noriko Nakamura, Hideki Hirakawa, Shusei Sato, Shungo Otagaki, Shogo Matsumoto, Satoshi Tabata, Yoshikazu Tanaka
The draft genome sequence of a wild rose (Rosa multiflora Thunb.) was determined using Illumina MiSeq and HiSeq platforms. The total length of the scaffolds was 739,637,845 bp, consisting of 83,189 scaffolds, which was close to the 711 Mbp length estimated by k-mer analysis. N50 length of the scaffolds was 90,830 bp, and extent of the longest was 1,133,259 bp. The average GC content of the scaffolds was 38.9%. After gene prediction, 67,380 candidates exhibiting sequence homology to known genes and domains were extracted, which included complete and partial gene structures...
October 16, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/29045601/genomic-changes-associated-with-reproductive-and-migratory-ecotypes-in-sockeye-salmon-oncorhynchus-nerka
#13
Andrew J Veale, Michael A Russello
Mechanisms underlying adaptive evolution can best be explored using paired populations displaying similar phenotypic divergence, illuminating the genomic changes associated with specific life history traits. Here we used paired migratory [anadromous vs. resident (kokanee)] and reproductive [shore- vs. stream-spawning] ecotypes of sockeye salmon (Oncorhynchus nerka) sampled from seven lakes and two rivers spanning three catchments (Columbia, Fraser, and Skeena) in British Columbia, Canada to investigate the patterns and processes underlying their divergence...
October 13, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29045579/auditory-frequency-representations-in-human-somatosensory-cortex
#14
Alexis Pérez-Bellido, Kelly Anne Barnes, Lexi E Crommett, Jeffrey M Yau
Recent studies have challenged the traditional notion of modality-dedicated cortical systems by showing that audition and touch evoke responses in the same sensory brain regions. While much of this work has focused on somatosensory responses in auditory regions, fewer studies have investigated sound responses and representations in somatosensory regions. In this functional magnetic resonance imaging (fMRI) study, we measured BOLD signal changes in participants performing an auditory frequency discrimination task and characterized activation patterns related to stimulus frequency using both univariate and multivariate analysis approaches...
October 13, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/29045563/generation-of-transgenic-marmosets-using-a-tetracyclin-inducible-transgene-expression-system-as-a-neurodegenerative-disease-model
#15
Ikuo Tomioka, Naotake Nogami, Terumi Nakatani, Kensuke Owari, Naoko Fujita, Hideyuki Motohashi, Osamu Takayama, Kentaro Takae, Yoshitaka Nagai, Kazuhiko Seki
Controllable transgene expression systems are indispensable tools for the production of animal models of disease to investigate protein functions at defined periods. However, in non-human primates that share genetic, physiological, and morphological similarities with humans, genetic modification techniques have not been well established; therefore, the establishment of novel transgenic models with controllable transgene expression systems will be valuable tools to understand pathological mechanism of human disease...
October 13, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29045518/negative-hyper-selection-of-metastatic-colorectal-cancer-patients-for-anti-egfr-monoclonal-antibodies-the-pressing-case-control-study
#16
C Cremolini, F Morano, R Moretto, R Berenato, E Tamborini, F Perrone, D Rossini, A Gloghini, A Busico, G Zucchelli, C Baratelli, E Tamburini, M Tampellini, E Sensi, G Fucà, C Volpi, M Milione, M Di Maio, G Fontanini, F De Braud, A Falcone, F Pietrantonio
Background: Refining the selection of metastatic colorectal cancer (mCRC) patients candidates for anti-EGFR monoclonal antibodies beyond RAS and BRAF testing is a challenge of precision oncology. Several uncommon genomic mechanisms of primary resistance, leading to activation of tyrosine kinase receptors other than EGFR or downstream signaling pathways, have been suggested by preclinical and retrospective studies. Patients and methods: We conducted this multicenter, prospective, case-control study to demonstrate the negative predictive impact of a panel of rare genomic alterations (PRESSING panel), including HER2/MET amplifications, ALK/ROS1/NTRK1-3/RET fusions, and HER2/PI3K/PTEN/AKT1 mutations...
September 25, 2017: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/29045505/surfaceome-profiling-enables-isolation-of-cancer-specific-exosomal-cargo-in-liquid-biopsies-from-pancreatic-cancer-patients
#17
J Castillo, V Bernard, F A San Lucas, K Allenson, M Capello, D U Kim, P Gascoyne, F C Mulu, B M Stephens, J Huang, H Wang, A A Momin, R O Jacamo, M Katz, R Wolff, M Javle, G Varadhachary, I I Wistuba, S Hanash, A Maitra, H Alvarez
Background: Detection of circulating tumor DNA (ctDNA) can be limited due to their relative scarcity in circulation, particularly while patients are actively undergoing therapy. Exosomes provide a vehicle through which cancer-specific material can be enriched from the compendium of circulating non-neoplastic tissue-derived nucleic acids. We performed a comprehensive profiling of the pancreatic ductal adenocarcinoma (PDAC) exosomal "surfaceome" in order to identify surface proteins that will render liquid biopsies amenable to cancer-derived exosome enrichment for downstream molecular profiling...
September 25, 2017: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/29045465/identifying-protein-complex-by-integrating-characteristic-of-core-attachment-into-dynamic-ppi-network
#18
Xianjun Shen, Li Yi, Xingpeng Jiang, Tingting He, Jincai Yang, Wei Xie, Po Hu, Xiaohua Hu
How to identify protein complex is an important and challenging task in proteomics. It would make great contribution to our knowledge of molecular mechanism in cell life activities. However, the inherent organization and dynamic characteristic of cell system have rarely been incorporated into the existing algorithms for detecting protein complexes because of the limitation of protein-protein interaction (PPI) data produced by high throughput techniques. The availability of time course gene expression profile enables us to uncover the dynamics of molecular networks and improve the detection of protein complexes...
2017: PloS One
https://www.readbyqxmd.com/read/29045442/a-semi-synthetic-glycosaminoglycan-analogue-inhibits-and-reverses-plasmodium-falciparum-cytoadherence
#19
Mark A Skidmore, Khairul Mohd Fadzli Mustaffa, Lynsay C Cooper, Scott E Guimond, Edwin A Yates, Alister G Craig
A feature of mature Plasmodium falciparum parasitized red blood cells is their ability to bind surface molecules of the microvascular endothelium via the parasite-derived surface protein Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1). This ligand is associated with the cytoadherence pathology observed in severe malaria. As pRBC treated with effective anti-malarial drugs are still able to cytoadhere, there is therefore a need to find an adjunct treatment that can inhibit and reverse the adhesion process...
2017: PloS One
https://www.readbyqxmd.com/read/29045433/integrated-mrna-and-microrna-transcriptome-analyses-reveal-regulation-of-thermal-acclimation-in-gymnocypris-przewalskii-a-case-study-in-tibetan-schizothoracine-fish
#20
Cunfang Zhang, Chao Tong, Fei Tian, Kai Zhao
Environmental acclimation is important episode in wildlife occupation of the high-altitude Tibetan Plateau (TP). Transcriptome-wide studies on thermal acclimation mechanism in fish species are rarely revealed in Tibetan Plateau fish at high altitude. Thus, we used mRNA and miRNA transcriptome sequencing to investigate regulation of thermal acclimation in larval Tibetan naked carp, Gymnocypris przewalskii. We first remodeled the regulation network of mRNA and miRNA in thermal acclimation, and then identified differential expression of miRNAs and target mRNAs enriched in metabolic and digestive pathways...
2017: PloS One
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