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https://www.readbyqxmd.com/read/29620027/two-polymorphs-of-2-5-dichloro-3-6-bis-dibenzylamino-p-hydroquinone-with-flexible-dibenzylamino-groups
#1
In Sub Shin, Yuta Shimada, Emi Horiguchi-Babamoto, Shinya Matsumoto
We obtained two conformational polymorphs of 2,5-dichloro-3,6-bis(dibenzylamino)-p-hydroquinone, C34 H30 Cl2 N2 O2 . Both polymorphs have an inversion centre at the centre of the hydroquinone ring (Z' = 1/2), and there are no significant differences between their bond lengths and angles. The most significant structural difference in the molecular conformations was found in the rotation of the phenyl rings of the two crystallographically independent benzyl groups. The crystal structures of the polymorphs were distinguishable with respect to the arrangement of the hydroquinone rings and the packing motif of the phenyl rings that form part of the benzyl groups...
April 1, 2018: Acta Crystallographica. Section C, Structural Chemistry
https://www.readbyqxmd.com/read/29593223/latitudinal-trends-in-human-primary-activities-characterizing-the-winter-day-as-a-synchronizer
#2
José María Martín-Olalla
This work analyzes time use surveys from 19 countries (17 European and 2 American) in the middle latitude (38-61 degree) accounting for 45% of world population in this range. Time marks for primary activities are contrasted against light/dark conditions. The analysis reveals winter sunrise synchronizes labor start time below 54 degree, occurring within winter civil twilight. Winter sunset is a source of synchronization for labor end times. Winter terminator punctuate meal times in Europe: dinner occurs 3 h after winter sunset time within 1 h; 40% narrower than variability of dinner local times...
March 28, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29589963/serotyping-and-genotyping-characterization-of-pathogenic-escherichia-coli-strains-in-kimchi-and-determination-of-their-kinetic-behavior-in-cabbage-kimchi-during-fermentation
#3
Yukyung Choi, Soomin Lee, Hyun Jung Kim, Heeyoung Lee, Sejeong Kim, Jeeyeon Lee, Jimyeong Ha, Hyemin Oh, Jang Won Yoon, Yohan Yoon, Kyoung-Hee Choi
This study determined the serotyping and genotyping properties of Escherichia coli strains isolated from kimchi and various raw vegetables used for kimchi preparation. In addition, the kinetic behavior of E. coli strains in kimchi during fermentation was also determined using a predictive microbiological model. The study results revealed that E. coli isolated from napa cabbage (3.3%; 1/30) was enterohemorrhagic E. coli (O6:H34), and eight typical colonies isolated from kimchi (15%; 6/40) were enteropathogenic E...
March 28, 2018: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29571838/multidrug-resistant-infections-in-long-term-care-facilities-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-and-hypervirulent-antibiotic-resistant-clostridium-difficile
#4
Maria Giufrè, Marisa Accogli, Enrico Ricchizzi, Fabrizio Barbanti, Claudio Farina, Paolo Fazii, Romano Mattei, Mario Sarti, Agostino Barozzi, Rossella Buttazzi, Marina Cosentino, Maria Nardone, Vincenzo Savini, Patrizia Spigaglia, Maria Luisa Moro, Marina Cerquetti
Infections due to multidrug-resistant (MDR) organisms in long-term care facilities (LTCFs) residents constitute a public health concern. This multicenter study investigated the frequency of ESBL-producing pathogens and MDR Clostridium difficile in clinical specimens from LTCF residents in Italy. During October 2014-March 2015, all urine and diarrheic fecal samples from LTCF residents (≥65 years) with suspected urinary tract infection or C. difficile infection, respectively, received for diagnosis by 4 hospital laboratories located in different cities were analyzed...
February 26, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29503639/comprehensive-molecular-characterization-of-escherichia-coli-isolates-from-urine-samples-of-hospitalized-patients-in-rio-de-janeiro-brazil
#5
Ana Carolina C Campos, Nathália L Andrade, Mithila Ferdous, Monika A Chlebowicz, Carla C Santos, Julio C D Correal, Jerome R Lo Ten Foe, Ana Cláudia P Rosa, Paulo V Damasco, Alex W Friedrich, John W A Rossen
Urinary tract infections (UTIs) are often caused by Escherichia coli . Their increasing resistance to broad-spectrum antibiotics challenges the treatment of UTIs. Whereas, E. coli ST131 is often multidrug resistant (MDR), ST69 remains susceptible to antibiotics such as cephalosporins. Both STs are commonly linked to community and nosocomial infections. E. coli phylogenetic groups B2 and D are associated with virulence and resistance profiles making them more pathogenic. Little is known about the population structure of E...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29442665/preparation-of-expoxy-functionalized-magnetic-nanoparticles-for-immobilization-of-glycerol-dehydrogenase
#6
Jian Gao, Ai-Rong Wang, Xiao-Ping Jiang, Jiu-Xun Zhang, Ye-Wang Zhang
Immobilization of glycerol dehydrogenase (GDH) from Serratia marcescens H30 onto epoxy functional magnetic nanoparticles by covalent attachment was carried out. The optimal immobilization conditions were obtained as follows: enzyme/support 6.08 mg/g, temperature 25 °C, pH 7.0 and time 8 h. Under these conditions, a high immobilization yield above 90% was obtained. The characterization of the immobilized GDH indicated that enhanced pH and thermal stability were achieved. Kinetic parameters Km of free and immobilized GDH were determined as 10...
July 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29302257/elisa-for-quantitative-determination-of-hepatitis-b-virus-surface-antigen
#7
Se-Ho Kim
Several studies have reported a good correlation between levels of serum hepatitis B virus surface antigen (HBsAg) and covalently closed circular DNA (cccDNA) before and after antiviral therapy. As a result, the quantification of HBsAg levels has attracted much attention in recent years as an important approach to evaluate viral activity. In this study, mAbs against HBsAg were generated and 9 mAbs (H17, H30, H31, H67, H73, H97, H101, H118, and H128) were investigated for optimization of HBsAg quantitation ELISA...
December 2017: Immune Network
https://www.readbyqxmd.com/read/29260847/manipulating-the-defect-structure-v-o-of-in-2-o-3-nanoparticles-for-enhancement-of-formaldehyde-detection
#8
Fubo Gu, Chunju Li, Dongmei Han, Zhihua Wang
Because defects such as oxygen vacancies (VO ) can affect the properties of nanomaterials, investigating the defect structure-function relationship are attracting intense attention. However, it remains an enormous challenge to the synthesis of nanomaterials with high sensing performance by manipulating VO because understanding the role of surface or bulk VO on the sensing properties of metal oxides is still missing. Herein, In2 O3 nanoparticles with different contents of surface and bulk VO were obtained by hydrogen reduction treatment, and the role of surface or bulk VO on the sensing properties of In2 O3 was investigated...
January 10, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29190804/virulence-genes-and-subclone-status-as-markers-of-experimental-virulence-in-a-murine-sepsis-model-among-escherichia-coli-sequence-type-131-clinical-isolates-from-spain
#9
Irene Merino, Stephen B Porter, Brian D Johnston, Connie Clabots, Evelyn Shaw, Juan Pablo Horcajada, Rafael Cantón, Patricia Ruiz-Garbajosa, James R Johnson
OBJECTIVE: To assess experimental virulence among sequence type 131 (ST131) Escherichia coli bloodstream isolates in relation to virulence genotype and subclone. METHODS: We analysed 48 Spanish ST131 bloodstream isolates (2010) by PCR for ST131 subclone status (H30Rx, H30 non-Rx, or non-H30), virulence genes (VGs), and O-type. Then we compared these traits with virulence in a murine sepsis model, as measured by illness severity score (ISS) and rapid lethality (mean ISS ≥ 4)...
2017: PloS One
https://www.readbyqxmd.com/read/29180984/risk-of-transmission-of-antimicrobial-resistant-escherichia-coli-from-commercial-broiler-and-free-range-retail-chicken-in-india
#10
Arif Hussain, Sabiha Shaik, Amit Ranjan, Nishant Nandanwar, Sumeet K Tiwari, Mohammad Majid, Ramani Baddam, Insaf A Qureshi, Torsten Semmler, Lothar H Wieler, Mohammad A Islam, Dipshikha Chakravortty, Niyaz Ahmed
Multidrug-resistant Escherichia coli infections are a growing public health concern. This study analyzed the possibility of contamination of commercial poultry meat (broiler and free-range) with pathogenic and or multi-resistant E. coli in retail chain poultry meat markets in India. We analyzed 168 E. coli isolates from broiler and free-range retail poultry (meat/ceca) sampled over a wide geographical area, for their antimicrobial sensitivity, phylogenetic groupings, virulence determinants, extended-spectrum-β-lactamase (ESBL) genotypes, fingerprinting by Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR and genetic relatedness to human pathogenic E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29167743/prevalence-and-characteristics-of-st131-clone-among-unselected-clinical-escherichia-coli-in-a-chinese-university-hospital
#11
Bin Li, Yanfang Lu, Fangjun Lan, Qingwen He, Chen Li, Yingping Cao
Background: Escherichia coli clinical sequence type 131 (ST131) has emerged as an extensively antimicrobial resistant E. coli clonal group in recent years throughout the world. The aim of this study was to investigate the prevalence and molecular characteristics of ST131 among unselected E. coli clinical isolates in a Chinese university hospital. Methods: Seven hundred consecutive E. coli isolates were collected at a Chinese university hospital between 2014 and 2015...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29075838/a-new-molecular-approach-based-on-the-secondary-structure-of-ribosomal-rna-for-phylogenetic-analysis-of-mobilid-ciliates
#12
Yan-Hong Du, Yuan-Jun Zhao, Fa-Hui Tang
We analyzed the secondary structure of the small subunit (SSU) rRNA genes of Mobilida (Ciliophora, Peritrichia) and found that the secondary structures of some regions within the SSU-rRNA gene are distinct between the families Trichodinidae and Urceolariidae. Therefore, some of these important regions including H10, H11, H17, H47, H29, H30, H37, E10-1, H45-H46, and V4 (E23-4, E23-7) could be used as the barcodes for classification of these two families. In contrast, V4 (E23-1, E23-2) belongs to a hypervariable region and is not a good barcode at the genus level because of its great inter-specific variation...
October 26, 2017: Current Microbiology
https://www.readbyqxmd.com/read/29039274/different-escherichia-coli-b2-st131-clades-b-and-c-producing-extended-spectrum-%C3%AE-lactamases-esbl-colonizing-residents-of-portuguese-nursing-homes
#13
C Rodrigues, E Machado, S Fernandes, L Peixe, Â Novais
ESBL-producing Enterobacteriaceae and particularly Escherichia coli ST131 isolates producing CTX-M enzymes are commonly found colonizing the intestine of nursing home (NH) residents, but ST131 subclonal structure has been scarcely explored in this vulnerable population. Our goal was to perform a pilot study to assess the faecal carriage rate and epidemiological features of ESBL- and/or carbapenemase-producing Enterobacteriaceae (ESBL-E and CPE, respectively) among NH residents. For this purpose, faecal samples from residents at 4 different NHs in the North of Portugal (representing 9·5% of the residents' population, July 2014) were screened for ESBL-E and/or CPE by phenotypic and genotypic methods...
November 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/29020219/epidemiology-and-antimicrobial-resistance-characteristics-of-the-st131-h30-subclone-among-extraintestinal-escherichia-coli-collected-from-us-children
#14
Arianna Miles-Jay, Scott J Weissman, Amanda L Adler, Veronika Tchesnokova, Evgeni V Sokurenko, Janet G Baseman, Danielle M Zerr
Background: E. coli ST131-H30 is a globally important pathogen implicated in rising rates of multidrug resistance among E. coli causing extraintestinal infections. Previous studies have focused on adults, leaving the epidemiology of H30 among children undefined. Methods: We used clinical data and isolates from a case-control study of extended-spectrum cephalosporin-resistant E. coli conducted at four US children's hospitals to estimate the burden and identify host correlates of infection with H30...
September 15, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28935873/whole-genome-sequencing-molecular-typing-and-in-vivo-virulence-of-oxa-48-producing-escherichia-coli-isolates-including-st131-h30-rx-h22-and-h41-subclones
#15
María de Toro, Javier Fernández, Vanesa García, Azucena Mora, Jorge Blanco, Fernando de la Cruz, M Rosario Rodicio
Carbapenem-resistant Enterobacteriaceae, including the increasingly reported OXA-48 Escherichia coli producers, are an emerging public health threat worldwide. Due to their alarming detection in our healthcare setting and their possible presence in the community, seven OXA-48-producing, extraintestinal pathogenic E. coli were analysed by whole genome sequencing as well as conventional tools, and tested for in vivo virulence. As a result, five E. coli OXA-48-producing subclones were detected (O25:H4-ST131/PST43-fimH30-virotype E; O25:H4-ST131/PST9-fimH22-virotype D5, O16:H5-ST131/PST506-fimH41; O25:H5-ST83/PST207 and O9:H25-ST58/PST24)...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28874372/multiple-modes-of-action-of-a-monoclonal-antibody-against-multidrug-resistant-escherichia-coli-sequence-type-131-h30
#16
Luis M Guachalla, Katharina Hartl, Cecília Varga, Lukas Stulik, Irina Mirkina, Stefan Malafa, Eszter Nagy, Gábor Nagy, Valéria Szijártó
The multidrug-resistant H30 subclone of extraintestinal pathogenic Escherichia coli sequence type 131 (ST131-H30) has spread worldwide. This clone expresses a conserved lipopolysaccharide (LPS) O antigen, O25b. Previously, we described monoclonal antibodies (MAbs) specific to the O25b antigen and characterized them as diagnostic and therapeutic tools. In this study, evidence is provided that besides the previously shown complement-mediated bactericidal effect, an O25b-specific humanized MAb, A1124, also enhances opsonophagocytic uptake by the murine macrophage cell line RAW 264...
November 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28833256/noncovalent-interactions-between-cisplatin-and-graphene-prototypes
#17
Ma Del Refugio Cuevas-Flores, Marco Antonio Garcia-Revilla, Massimiliano Bartolomei
Cisplatin (CP) has been widely used as an anticancer drug for more than 30 years despite severe side effects due to its low bioavailability and poor specificity. For this reason, it is paramount to study and design novel nanomaterials to be used as vectors capable to effectively deliver the drug to the biological target. The CP square-planar geometry, together with its low water solubility, suggests that it could be possibly easily adsorbed on 2D graphene nanostructures through the interaction with the related highly conjugated π-electron system...
August 21, 2017: Journal of Computational Chemistry
https://www.readbyqxmd.com/read/28825894/adhesion-and-invasion-to-epithelial-cells-and-motility-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-reveal-st131-superiority-a-comparative-in-vitro-study-of-extraintestinal-pathogenic-e-coli-lineages
#18
Kira Kondratyeva, Ayala Wollman, Gabi Gerlitz, Shiri Navon-Venezia
PURPOSE: Extended-spectrum β-lactamase (ESBL)-producing extraintestinal pathogenic Escherichia coli (ExPEC) sequence type ST131 is pandemic, and it is the major contributor to antibiotic resistance in E. coli. Despite its epidemiological superiority, the physiological reasons that decipher its success remain elusive. We aimed to compare the adhesion, invasion and motility potential of ST131 versus other E. coli lineages. METHODOLOGY: In this in vitro comparative study, 14 ESBL-producing ExPEC community-onset bacteremia isolates were chosen from a reported clinical collection (Karfunkel D, Carmeli Y, Chmelnitsky I, Kotlovsky T, Navon-Venezia S...
September 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28742224/synthesis-of-unimolecular-micelles-with-incorporated-hyperbranched-boltorn-h30-polyester-modified-with-hyperbranched-helical-poly-phenyl-isocyanide-chains-and-their-enantioselective-crystallization-performance
#19
Zhi-Huang Zhang, Chen-Yang Qiao, Jian Zhang, Wen-Ming Zhang, Jun Yin, Zong-Quan Wu
Here, the fabrication of unimolecular micelles functionalized with helical polymeric chains as a chiral nucleating agent in enantioselective crystallization is reported. Starting from a fractionated hyperbranched polyester (Boltorn H30), the ring-opening polymerization of l-lactic acid (LLA) and subsequent terminal-group modification affords the alkyne-Pd(II)-anchored hyperbranched macroinitiator (H30-PLLA-Pd). By taking advantage of a Pd(II)-catalyzed living polymerization of chiral pendant modified l- or d-phenyl isocyanide (PI) monomers, well-defined chiral unimolecular micelles (H30-PLLA-PPI) grafted with radiating helical PPI coronas of one predominant screw sense are obtained...
July 25, 2017: Macromolecular Rapid Communications
https://www.readbyqxmd.com/read/28708540/influence-of-two-ovulation-inducing-agents-on-the-pituitary-response-and-follicle-blood-flow-in-mares
#20
Y L Boakari, J C Ferreira, H S Canesin, D L Thompson, F S Lima, J C F Pantoja, C Meira
The objective of the current study was to evaluate the effects of deslorelin and hCG, two ovulation-inducing therapies, on LH surge and follicle vascularity in mares. Thirty mares were either treated with 1.5 mg IM of deslorelin, 2,500 IU IV of hCG or 2 mL IM of NaCl 0.9% (GnRH, hCG and Saline groups, respectively). Power-flow Doppler examination and blood collection were performed every hour during the first 12 hours after treatment (H0) and every six hours between hours 12 (H12) and 30 (H30) after treatment...
June 8, 2017: Theriogenology
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