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https://www.readbyqxmd.com/read/29039274/different-escherichia-coli-b2-st131-clades-b-and-c-producing-extended-spectrum-%C3%AE-lactamases-esbl-colonizing-residents-of-portuguese-nursing-homes
#1
C Rodrigues, E Machado, S Fernandes, L Peixe, Â Novais
ESBL-producing Enterobacteriaceae and particularly Escherichia coli ST131 isolates producing CTX-M enzymes are commonly found colonizing the intestine of nursing home (NH) residents, but ST131 subclonal structure has been scarcely explored in this vulnerable population. Our goal was to perform a pilot study to assess the faecal carriage rate and epidemiological features of ESBL- and/or carbapenemase-producing Enterobacteriaceae (ESBL-E and CPE, respectively) among NH residents. For this purpose, faecal samples from residents at 4 different NHs in the North of Portugal (representing 9·5% of the residents' population, July 2014) were screened for ESBL-E and/or CPE by phenotypic and genotypic methods...
October 17, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/29020219/epidemiology-and-antimicrobial-resistance-characteristics-of-the-st131-h30-subclone-among-extraintestinal-escherichia-coli-collected-from-us-children
#2
Arianna Miles-Jay, Scott J Weissman, Amanda L Adler, Veronika Tchesnokova, Evgeni V Sokurenko, Janet G Baseman, Danielle M Zerr
Background: E. coli ST131-H30 is a globally important pathogen implicated in rising rates of multidrug resistance among E. coli causing extraintestinal infections. Previous studies have focused on adults, leaving the epidemiology of H30 among children undefined. Methods: We used clinical data and isolates from a case-control study of extended-spectrum cephalosporin-resistant E. coli conducted at four US children's hospitals to estimate the burden and identify host correlates of infection with H30...
September 15, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28935873/whole-genome-sequencing-molecular-typing-and-in-vivo-virulence-of-oxa-48-producing-escherichia-coli-isolates-including-st131-h30-rx-h22-and-h41-subclones
#3
María de Toro, Javier Fernández, Vanesa García, Azucena Mora, Jorge Blanco, Fernando de la Cruz, M Rosario Rodicio
Carbapenem-resistant Enterobacteriaceae, including the increasingly reported OXA-48 Escherichia coli producers, are an emerging public health threat worldwide. Due to their alarming detection in our healthcare setting and their possible presence in the community, seven OXA-48-producing, extraintestinal pathogenic E. coli were analysed by whole genome sequencing as well as conventional tools, and tested for in vivo virulence. As a result, five E. coli OXA-48-producing subclones were detected (O25:H4-ST131/PST43-fimH30-virotype E; O25:H4-ST131/PST9-fimH22-virotype D5, O16:H5-ST131/PST506-fimH41; O25:H5-ST83/PST207 and O9:H25-ST58/PST24)...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28874372/triple-edged-sword-multiple-modes-of-action-of-a-monoclonal-antibody-against-the-multi-drug-resistant-escherichia-coli-clone-st131-h30
#4
Luis M Guachalla, Katharina Hartl, Cecília Varga, Lukas Stulik, Irina Mirkina, Stefan Malafa, Eszter Nagy, Gábor Nagy, Valéria Szijártó
The multi-drug resistant extra-intestinal pathogenic Escherichia coli clone, ST131-H30 has spread worldwide. This clone expresses a conserved LPS O-antigen, O25b. Previously, we described monoclonal antibodies (mAbs) specific to the O25b antigen and characterized them as diagnostic and therapeutic tools. In this study evidence is provided that besides the previously shown complement mediated bactericidal effect, an O25b-specific humanized mAb, A1124 also enhances opsonophagocytic uptake by the murine macrophage cell line RAW264...
September 5, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28833256/noncovalent-interactions-between-cisplatin-and-graphene-prototypes
#5
Ma Del Refugio Cuevas-Flores, Marco Antonio Garcia-Revilla, Massimiliano Bartolomei
Cisplatin (CP) has been widely used as an anticancer drug for more than 30 years despite severe side effects due to its low bioavailability and poor specificity. For this reason, it is paramount to study and design novel nanomaterials to be used as vectors capable to effectively deliver the drug to the biological target. The CP square-planar geometry, together with its low water solubility, suggests that it could be possibly easily adsorbed on 2D graphene nanostructures through the interaction with the related highly conjugated π-electron system...
August 21, 2017: Journal of Computational Chemistry
https://www.readbyqxmd.com/read/28825894/adhesion-and-invasion-to-epithelial-cells-and-motility-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-reveal-st131-superiority-a-comparative-in-vitro-study-of-extraintestinal-pathogenic-e-coli-lineages
#6
Kira Kondratyeva, Ayala Wollman, Gabi Gerlitz, Shiri Navon-Venezia
PURPOSE: Extended-spectrum β-lactamase (ESBL)-producing extraintestinal pathogenic Escherichia coli (ExPEC) sequence type ST131 is pandemic, and it is the major contributor to antibiotic resistance in E. coli. Despite its epidemiological superiority, the physiological reasons that decipher its success remain elusive. We aimed to compare the adhesion, invasion and motility potential of ST131 versus other E. coli lineages. METHODOLOGY: In this in vitro comparative study, 14 ESBL-producing ExPEC community-onset bacteremia isolates were chosen from a reported clinical collection (Karfunkel D, Carmeli Y, Chmelnitsky I, Kotlovsky T, Navon-Venezia S...
September 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28742224/synthesis-of-unimolecular-micelles-with-incorporated-hyperbranched-boltorn-h30-polyester-modified-with-hyperbranched-helical-poly-phenyl-isocyanide-chains-and-their-enantioselective-crystallization-performance
#7
Zhi-Huang Zhang, Chen-Yang Qiao, Jian Zhang, Wen-Ming Zhang, Jun Yin, Zong-Quan Wu
Here, the fabrication of unimolecular micelles functionalized with helical polymeric chains as a chiral nucleating agent in enantioselective crystallization is reported. Starting from a fractionated hyperbranched polyester (Boltorn H30), the ring-opening polymerization of l-lactic acid (LLA) and subsequent terminal-group modification affords the alkyne-Pd(II)-anchored hyperbranched macroinitiator (H30-PLLA-Pd). By taking advantage of a Pd(II)-catalyzed living polymerization of chiral pendant modified l- or d-phenyl isocyanide (PI) monomers, well-defined chiral unimolecular micelles (H30-PLLA-PPI) grafted with radiating helical PPI coronas of one predominant screw sense are obtained...
July 25, 2017: Macromolecular Rapid Communications
https://www.readbyqxmd.com/read/28708540/influence-of-two-ovulation-inducing-agents-on-the-pituitary-response-and-follicle-blood-flow-in-mares
#8
Y L Boakari, J C Ferreira, H S Canesin, D L Thompson, F S Lima, J C F Pantoja, C Meira
The objective of the current study was to evaluate the effects of deslorelin and hCG, two ovulation-inducing therapies, on LH surge and follicle vascularity in mares. Thirty mares were either treated with 1.5 mg IM of deslorelin, 2,500 IU IV of hCG or 2 mL IM of NaCl 0.9% (GnRH, hCG and Saline groups, respectively). Power-flow Doppler examination and blood collection were performed every hour during the first 12 hours after treatment (H0) and every six hours between hours 12 (H12) and 30 (H30) after treatment...
June 8, 2017: Theriogenology
https://www.readbyqxmd.com/read/28638846/the-pandemic-h30-subclone-of-sequence-type-131-st131-as-the-leading-cause-of-multidrug-resistant-escherichia-coli-infections-in-the-united-states-2011-2012
#9
James R Johnson, Stephen Porter, Paul Thuras, Mariana Castanheira
BACKGROUND: Extraintestinal Escherichia coli infections are increasingly challenging due to emerging antimicrobial resistance, including resistance to extended-spectrum beta-lactams and fluoroquinolones. Sequence type 131 (ST131) is a leading contributor. METHODS: Three hundred sixty E. coli clinical isolates from across the United States (2011-2012), selected randomly from the SENTRY collection within 3 resistance categories (extended-spectrum cephalosporin [ECS]-reduced susceptibility [RS]; fluoroquinolone-resistant, ESC-susceptible; and fluoroquinolone-susceptible, ESC-susceptible) were typed for phylogroup, sequence type complex (STc), subsets thereof, virulence genotype, O type, and beta-lactamase genes...
2017: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/28630063/listeria-vectored-vaccine-expressing-the-mycobacterium-tuberculosis-30-kda-major-secretory-protein-via-the-constitutively-active-prfa-regulon-boosts-bcg-efficacy-against-tuberculosis
#10
Qingmei Jia, Barbara Jane Dillon, Saša Masleša-Galić, Marcus A Horwitz
A potent vaccine against tuberculosis, one of the world's deadliest diseases, is needed to enhance the immunity of people worldwide, most of whom have been vaccinated with the partially effective BCG vaccine. Here we investigate novel live attenuated recombinant Listeria monocytogenes (rLm) vaccines expressing the Mycobacterium tuberculosis (Mtb) 30 kDa major secretory protein (r30/Ag85B) (rLm30) as heterologous booster vaccines in animals primed with BCG. Using three attenuated Lm vectors, rLm ΔactA (LmI), rLm ΔactA ΔinlB (LmII), and rLm ΔactA ΔinlBprfA* (LmIII), we constructed five rLm30 vaccine candidates expressing the r30 linked in-frame to the Lm Listeriolycin O signal sequence and driven by the hly promoter (h30) or linked in-frame to the ActA N-terminus and driven by the actA promoter (a30)...
June 19, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28592545/development-of-a-web-tool-for-escherichia-coli-subtyping-based-on-fimh-alleles
#11
Louise Roer, Veronika Tchesnokova, Rosa Allesøe, Mariya Muradova, Sujay Chattopadhyay, Johanne Ahrenfeldt, Martin C F Thomsen, Ole Lund, Frank Hansen, Anette M Hammerum, Evgeni Sokurenko, Henrik Hasman
The aim of this study was to construct a valid publicly available method for in silico fimH subtyping of Escherichia coli particularly suitable for differentiation of fine-resolution subgroups within clonal groups defined by standard multilocus sequence typing (MLST). FimTyper was constructed as a FASTA database containing all currently known fimH alleles. The software source code is publicly available at https://bitbucket.org/genomicepidemiology/fimtyper, the database is freely available at https://bitbucket...
August 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28584160/rapid-identification-of-different-escherichia-coli-sequence-type-131-clades
#12
Yasufumi Matsumura, Johann D D Pitout, Gisele Peirano, Rebekah DeVinney, Taro Noguchi, Masaki Yamamoto, Ryota Gomi, Tomonari Matsuda, Satoshi Nakano, Miki Nagao, Michio Tanaka, Satoshi Ichiyama
Escherichia coli sequence type 131 (ST131) is a pandemic clonal lineage that is responsible for the global increase in fluoroquinolone resistance and extended-spectrum-β-lactamase (ESBL) producers. The members of ST131 clade C, especially subclades C2 and C1-M27, are associated with ESBLs. We developed a multiplex conventional PCR assay with the ability to detect all ST131 clades (A, B, and C), as well as C subclades (C1-M27, C1-nM27 [C1-non-M27], and C2). To validate the assay, we used 80 ST131 global isolates that had been fully sequenced...
August 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28552831/extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-isolated-from-healthy-humans-in-mexico-including-subclone-st131-b2-o25-h4-h30-rx
#13
Gerardo Cortés-Cortés, Patricia Lozano-Zarain, Carmen Torres, Carla Andrea Alonso, Ana María Ríos-Torres, Miguel Castañeda, Liliana López-Pliego, Armando Navarro, Rosa Del Carmen Rocha-Gracia
OBJECTIVES: The resistance mechanisms, molecular type and plasmid content of cefotaxime-resistant Escherichia coli isolated from faecal samples of healthy volunteers in Puebla, Mexico, were characterised. METHODS AND RESULTS: Cefotaxime-resistant E. coli were recovered from 11 (18%) of 60 healthy volunteers. The isolates (one per sample) were characterised as multidrug-resistant and phenotypically extended-spectrum β-lactamase (ESBL)-producing strains. Genes encoding resistance to β-lactams (blaCTX-M-15, blaCTX-M-14a, blaCTX-M-14b, blaOXA-1, blaTEM-1b), quinolones [aac(6')-Ib-cr, qnrB19], aminoglycosides [aac(3')-II] and tetracycline [tet(A), tet(B)] were detected among the 11 ESBL-producing E...
June 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28550370/molecular-epidemiology-of-escherichia-coli-sequence-type-131-and-its-h30-h30-rx-subclones-recovered-from-extra-intestinal-infections-first-report-of-oxa-48-producing-st131-clone-from-iran
#14
Z Hojabri, M Mirmohammadkhani, F Kamali, K Ghassemi, S Taghavipour, O Pajand
Multidrug-resistant (MDR) O25b-ST131 clone of Escherichia coli is well established as a significant cause of extra-intestinal infections worldwide. However, there have been no studies about the prevalence of ST131 and its H30/H30Rx subclones from Iran. The prevalence of ST131 was 29.8% among phylogroups B2, D, and F of E.coli isolates recovered from extra-intestinal infections. Fifty-seven (90.4%) and six (9.6%) of isolates belonged to serogroups O25b and O16 respectively, and exhibited high rates of MDR (98...
May 27, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28533233/epidemic-emergence-in-the-united-states-of-escherichia-coli-sequence-type-131-h30-st131-h30-2000-to-2009
#15
James R Johnson, Stephen Porter, Paul Thuras, Mariana Castanheira
The H30 subclone of Escherichia coli sequence type 131 (ST131-H30) has become the leading antimicrobial resistance E. coli lineage in the United States and often exhibits resistance to one or both of the two key antimicrobial classes for treating Gram-negative infections, extended-spectrum cephalosporins (ESCs) and fluoroquinolones (FQs). However, the timing of and reasons for its recent emergence are inadequately defined. Accordingly, from E. coli clinical isolates collected systematically across the United States by the SENTRY Antimicrobial Surveillance Program in 2000, 2003, 2006, and 2009, 234 isolates were selected randomly, stratified by year, within three resistance categories: (i) ESC-reduced susceptibility, regardless of FQ phenotype (ESC-RS); (ii) FQ resistance, ESC susceptible (FQ-R); and (iii) FQ susceptible, ESC susceptible (FQ-S)...
August 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28439032/inactivation-of-transcriptional-regulators-during-within-household-evolution-of-escherichia-coli
#16
Dagmara I Kisiela, Matthew Radey, Sandip Paul, Stephen Porter, Kseniya Polukhina, Veronika Tchesnokova, Sofiya Shevchenko, Diana Chan, Maliha Aziz, Timothy J Johnson, Lance B Price, James R Johnson, Evgeni V Sokurenko
We analyzed the within-household evolution of two household-associated Escherichia coli strains from pandemic clonal group ST131-H30, using isolates recovered from five individuals within two families, each of which had a distinct strain. Family 1's strain was represented by a urine isolate from the index patient (older sister) with recurrent cystitis and a blood isolate from her younger sister with fatal urosepsis. Family 2's strain was represented by a urine isolate from the index patient (father) with pyelonephritis and renal abscesses, blood and kidney drainage isolates from the daughter with emphysematous pyelonephritis, and urine and fecal isolates from the mother with cystitis...
July 1, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28429685/interlayer-crosslinked-micelles-prepared-from-star-shaped-copolymers-via-click-chemistry-for-sustained-drug-release
#17
Xiaojin Zhang, Hongquan Wang, Yu Dai
To balance the stability and the particle size of polymeric micelles, star-shaped copolymers Hx-yne-N3-PEG containing both alkynyl and azido groups were synthesized from hyperbranched 2,2-bismethylolpropionic acid polyester (H20 with 16 hydroxyl, H30 with 32 hydroxyl, H40 with 64 hydroxyl) to develop interlayer-crosslinked micelles by click chemistry. The results of dynamic light scattering indicate that the crosslinking could enhance the stability of polymeric micelles. The crosslinked micelles are regular nanosized (approximately 20 nm) spheres observed by a transmission electron microscope...
May 19, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28412380/colonization-by-multidrug-resistant-organisms-in-long-term-care-facilities-in-italy-a-point-prevalence-study
#18
M Giufrè, E Ricchizzi, M Accogli, F Barbanti, M Monaco, F Pimentel de Araujo, C Farina, P Fazii, R Mattei, M Sarti, A Barozzi, R Buttazzi, M Cosentino, M Nardone, V Savini, P Spigaglia, A Pantosti, M L Moro, M Cerquetti
OBJECTIVES: To determine prevalence and risk factors for colonization by multidrug-resistant organisms (MDROs) in long-term care facility (LTCF) residents in Italy. Genotypes of MDRO isolates were investigated. METHODS: A point-prevalence study was conducted at 12 LTCFs located in four Italian cities (2 February to 14 March 2015). Rectal swabs, faeces and nasal/auxiliary swabs were cultured for extended-spectrum β-lactamase (ESBL)- and/or carbapenemase-producing Enterobacteriaceae, Clostridium difficile and methicillin-resistant Staphylococcus aureus (MRSA) respectively...
April 12, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28347271/the-st131-escherichia-coli-h22-subclone-from-human-intestinal-microbiota-comparison-of-genomic-and-phenotypic-traits-with-those-of-the-globally-successful-h30-subclone
#19
Marie-Hélène Nicolas-Chanoine, Marie Petitjean, Azucena Mora, Noémie Mayer, Jean-Philippe Lavigne, Olivier Boulet, Véronique Leflon-Guibout, Jorge Blanco, Didier Hocquet
BACKGROUND: In 2006, we found healthy subjects carrying ST131 Escherichia coli in their intestinal microbiota consisting of two populations: a subdominant population of fluoroquinolone-resistant E. coli belonging to subclone H30 (H30-R or subclade C1), the current worldwide dominant ST131 subclone, and a dominant E. coli population composed of antibiotic-susceptible E. coli belonging to subclone H22 (clade B), the precursor of subclone H30. We sequenced the whole genome of fecal H22 strain S250, compared it to the genomes of ExPEC ST131 H30-Rx strain JJ1886 and commensal ST131 H41 strain SE15, sought the H22-H30 genomic differences in our fecal strains and assessed their phenotypic consequences...
March 27, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28257756/bacteraemia-due-to-non-esbl-producing-escherichia-coli-o25b-h4-sequence-type-131-insights-into-risk-factors-clinical-features-and-outcomes
#20
Isabel Morales-Barroso, Lorena López-Cerero, José Molina, Mar Bellido, María Dolores Navarro, Lara Serrano, Verónica González-Galán, Julia Praena, Alvaro Pascual, Jesús Rodríguez-Baño
The epidemiology and outcomes of bloodstream infections (BSIs) caused by Escherichia coli ST131 isolates not producing extended-spectrum β-lactamases (ESBLs) are not well defined despite being more prevalent than ESBL-producers. In this study, risk factors and the impact on outcome of BSIs caused by non-ESBL-producing ST131 E. coli versus non-ST131 E. coli were investigated. A case-control study was performed in two tertiary centres to identify risk factors for ST131. Molecular methods were used to investigate all E...
April 2017: International Journal of Antimicrobial Agents
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