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https://www.readbyqxmd.com/read/28438316/multiple-modes-of-capillary-electrophoresis-applied-in-peptide-nucleic-acid-related-study
#1
Xiaoqian Wang, Youhao Hu, Feng Qu, Rizwan Ullah Khan
Peptide Nucleic Acid (PNA) is a nucleic acid analogue, whose neutrally charged and hydrophobic backbone makes it more stable in vivo, so it might act as a potentially better protein probe as compared to aptamer. Currently the investigation of PNA and protein interaction is scarce. In this research, multiple modes of capillary electrophoresis were established and applied for PNA characterization and its interaction with ssDNA and protein. A 15-mer PNA having the same nucleobase sequence as 15-mer anti-thrombin DNA aptamer was chosen as PNA model for this study, its pI (7...
April 17, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28437577/high-gene-flow-despite-opposite-chirality-in-hybrid-zones-between-enantiomorphic-door-snails
#2
Eva L Koch, Marco T Neiber, Frank Walther, Bernhard Hausdorf
We studied differentiation and gene flow patterns between enantiomorphic door-snail species in two hybrid zones in the Bucegi Mountains (Romania) to investigate the effects of intrinsic barriers (complications in copulation) and extrinsic selection by environmental factors. A mitochondrial gene tree confirmed the historical separation of the examined populations into the dextral Alopia livida and the sinistral Alopia straminicollis in accordance with the morphological classification, but also indicated gene flow between the species...
April 24, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28428868/metapopulation-patterns-of-additive-and-nonadditive-genetic-variance-in-the-sea-bass-dicentrarchus-labrax
#3
Bruno Guinand, Marc Vandeputte, Mathilde Dupont-Nivet, Alain Vergnet, Pierrick Haffray, Hervé Chavanne, Béatrice Chatain
Describing and explaining the geographic within-species variation in phenotypes ("phenogeography") in the sea over a species distribution range is central to our understanding of a variety of eco-evolutionary topics. However, phenogeographic studies that have a large potential to investigate adaptive variation are overcome by phylogeographic studies, still mainly focusing on neutral markers. How genotypic and phenotypic data could covary over large geographic scales remains poorly understood in marine species...
April 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28428842/incipient-ecological-speciation-between-successional-varieties-of-a-dominant-tree-involves-intrinsic-postzygotic-isolating-barriers
#4
Elizabeth A Stacy, Bhama Paritosh, Melissa A Johnson, Donald K Price
Whereas disruptive selection imposed by heterogeneous environments can lead to the evolution of extrinsic isolating barriers between diverging populations, the evolution of intrinsic postzygotic barriers through divergent selection is less certain. Long-lived species such as trees may be especially slow to evolve intrinsic isolating barriers. We examined postpollination reproductive isolating barriers below the species boundary, in an ephemeral hybrid zone between two successional varieties of the landscape-dominant Hawaiian tree, Metrosideros polymorpha, on volcanically active Hawai'i Island...
April 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28424594/overexpression-of-human-sod1-leads-to-discrete-defects-in-the-cerebellar-architecture-in-the-mouse
#5
Pegah Afshar, Niloufar Ashtari, Xiaodan Jiao, Maryam Rahimi-Balaei, Xiaosha Zhang, Behzad Yaganeh, Marc R Del Bigio, Jiming Kong, Hassan Marzban
The human superoxide dismutase 1 (SOD1) gene is responsible for neutralizing supercharged oxygen radicals within the cell. Mutation in SOD1 gene causes amyotrophic lateral sclerosis (ALS). Recent studies have shown involvement of the cerebellum in ALS, although the cerebellar contribution in SOD1 transgenic mice remains unclear. Using immunohistopathology, we investigated the Purkinje cell phenotype in the vermis of the SOD1 transgenic mice cerebellum. Calbindin 1 (Calb1) and three well-known zone and stripe markers, zebrin II, HSP25, and PLCβ4 have been used to explore possible alteration in zone and stripe...
2017: Frontiers in Neuroanatomy
https://www.readbyqxmd.com/read/28418580/effect-of-surfactant-species-and-electrophoretic-medium-composition-on-the-electrophoretic-behavior-of-neutral-and-water-insoluble-linear-synthetic-polymers-in-nonaqueous-capillary-zone-electrophoresis
#6
Nao Fukai, Shinya Kitagawa, Hajime Ohtani
We have recently demonstrated the separation of neutral and water-insoluble linear synthetic polymers in non-aqueous capillary zone electrophoresis (NACZE) using a cationic surfactant of cetyltrimethylammonium chloride (CTAC). In this study, eight ionic surfactants were investigated for the separation of four synthetic polymers (polystyrene, polymethylmethacrylates, polybutadiene, and polycarbonate); only three surfactants (CTAC, dimethyldioctadecylammonium bromide, and sodium dodecylsulfate) caused their separation...
April 18, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28402181/chemical-composition-of-rosehips-from-different-rosa-species-an-alternative-source-of-antioxidants-for-food-industry
#7
Sandra Jiménez, Nerea Jiménez-Moreno, Asunción Luquin, Mariano Laguna, María Jesús Rodríguez-Yoldi, Carmen Ancín-Azpilicueta
It is important to explore new sources of natural additives because the demand for these compounds by consumers is increasing. These products also provide health benefits and help in food preservation. An unexplored source of nutrients and antioxidant compounds is rosehip, the fleshy fruit of roses. This work compares antioxidant compounds (vitamin C, neutral phenols and acidic phenols) content of four Rosa species rosehips: R. pouzinii, R. corymbifera, R. glauca and R. canina coming from different geographical zones...
April 12, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28379939/re-evaluation-of-learned-information-in-drosophila
#8
Johannes Felsenberg, Oliver Barnstedt, Paola Cognigni, Suewei Lin, Scott Waddell
Animals constantly assess the reliability of learned information to optimize their behaviour. On retrieval, consolidated long-term memory can be neutralized by extinction if the learned prediction was inaccurate. Alternatively, retrieved memory can be maintained, following a period of reconsolidation during which it is labile. Although extinction and reconsolidation provide opportunities to alleviate problematic human memories, we lack a detailed mechanistic understanding of memory updating. Here we identify neural operations underpinning the re-evaluation of memory in Drosophila...
April 13, 2017: Nature
https://www.readbyqxmd.com/read/28344495/enantioselective-analysis-of-fluoxetine-in-pharmaceutical-formulations-by-capillary-zone-electrophoresis
#9
Melania Cârcu-Dobrin, Monica Budău, Gabriel Hancu, Laszlo Gagyi, Aura Rusu, Hajnal Kelemen
Fluoxetine is an antidepressant, a selective serotonin reuptake inhibitor (SSRI) used primarily in the treatment of major depression, panic disorder and obsessive compulsive disorder. Chiral separation of racemic fluoxetine is necessary due to its enantioselective metabolism. In order to develop a suitable method for chiral separation of fluoxetine, cyclodextrin (CD) modified capillary electrophoresis (CE) was employed. A large number of native and derivatized, neutral and ionized CD derivatives were screened to find the optimal chiral selector...
March 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/28343575/expression-analysis-of-toll-like-receptors-and-interleukins-in-tharparkar-cattle-during-acclimation-to-heat-stress-exposure
#10
Jaya Bharati, S S Dangi, S R Mishra, V S Chouhan, V Verma, O Shankar, M K Bharti, A Paul, Dilip K Mahato, G Rajesh, G Singh, V P Maurya, S Bag, Puneet Kumar, M Sarkar
Six male Tharparkar cattle of 2-3 years old were selected for the study. After 15 days acclimation at thermo neutral zone (TNZ) in psychrometric chamber, animals were exposed at 42°C for 6h up to 23 days followed by 12 days of recovery period. Blood samples were collected during control period at TNZ (day 1, 5 and 12), after heat stress exposure (day 1-10, Short Term Heat Stress Acclimation - STHSA; day 15-23, Long Term Heat Stress Acclimation - LTHSA) and recovery period (day 7 and 12) and peripheral blood mononuclear cells (PBMCs) were isolated for RNA and protein extraction...
April 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/28314568/health-workers-and-the-weaponisation-of-health-care-in-syria-a-preliminary-inquiry-for-the-lancet-american-university-of-beirut-commission-on-syria
#11
Fouad M Fouad, Annie Sparrow, Ahmad Tarakji, Mohamad Alameddine, Fadi El-Jardali, Adam P Coutts, Nour El Arnaout, Lama Bou Karroum, Mohammed Jawad, Sophie Roborgh, Aula Abbara, Fadi Alhalabi, Ibrahim AlMasri, Samer Jabbour
The conflict in Syria presents new and unprecedented challenges that undermine the principles and practice of medical neutrality in armed conflict. With direct and repeated targeting of health workers, health facilities, and ambulances, Syria has become the most dangerous place on earth for health-care providers. The weaponisation of health care-a strategy of using people's need for health care as a weapon against them by violently depriving them of it-has translated into hundreds of health workers killed, hundreds more incarcerated or tortured, and hundreds of health facilities deliberately and systematically attacked...
March 14, 2017: Lancet
https://www.readbyqxmd.com/read/28306740/metabolic-rate-of-the-red-panda-ailurus-fulgens-a-dietary-bamboo-specialist
#12
Yuxiang Fei, Rong Hou, James R Spotila, Frank V Paladino, Dunwu Qi, Zhihe Zhang
The red panda (Ailurus fulgens) has a similar diet, primarily bamboo, and shares the same habitat as the giant panda, Ailuropoda melanoleuca. There are considerable efforts underway to understand the ecology of the red panda and to increase its populations in natural reserves. Yet it is difficult to design an effective strategy for red panda reintroduction if we do not understand its basic biology. Here we report the resting metabolic rate of the red panda and find that it is higher than previously measured on animals from a zoo...
2017: PloS One
https://www.readbyqxmd.com/read/28303246/cytotoxicity-and-antimicrobial-effects-of-a-new-fast-set-mta
#13
Michelle Shin, Jung-Wei Chen, Chi-Yang Tsai, Raydolfo Aprecio, Wu Zhang, Ji Min Yochim, Naichia Teng, Mahmoud Torabinejad
Purpose. To compare the biocompatibility and antimicrobial effectiveness of the new Fast-Set MTA (FS-MTA) with ProRoot MTA (RS-MTA). Methods. The agar overlay method with neutral red dye was used. L929 mouse fibroblast cells were cultured. The liquid and oil extracts and solid test material were placed on the agar overlay, four samples for each material. Phenol was used as the positive control and cottonseed oil and MEM extracts were used as negative controls. Cytotoxicity was examined by measuring the zones of decolorization and evaluating cell lysis under an inverted microscope using the established criteria after 24 and 48 hours...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28303177/community-assembly-in-lake-tanganyika-cichlid-fish-quantifying-the-contributions-of-both-niche-based-and-neutral-processes
#14
Thijs Janzen, Adriana Alzate, Moritz Muschick, Martine E Maan, Fons van der Plas, Rampal S Etienne
The cichlid family features some of the most spectacular examples of adaptive radiation. Evolutionary studies have highlighted the importance of both trophic adaptation and sexual selection in cichlid speciation. However, it is poorly understood what processes drive the composition and diversity of local cichlid species assemblages on relatively short, ecological timescales. Here, we investigate the relative importance of niche-based and neutral processes in determining the composition and diversity of cichlid communities inhabiting various environmental conditions in the littoral zone of Lake Tanganyika, Zambia...
February 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28259619/improving-adolescent-preventive-care-in-an-urban-pediatric-clinic-capturing-missed-opportunities
#15
Tina Kumra, Shweta Antani, Sara B Johnson, Sallie J Weaver
PURPOSE: To increase the proportion of adolescents with Medicaid who receive preventive care services in an urban pediatric clinic. METHODS: A quality improvement intervention was implemented at an urban pediatric primary care clinic between November 2013 and October 2014. The intervention systematically "flipped" acute visits into well-care visits for patients ages 12-21 years, when overdue. The primary process measure was the percentage of acute visits expanded to include well-care components out of total eligible opportunities...
March 1, 2017: Journal of Adolescent Health: Official Publication of the Society for Adolescent Medicine
https://www.readbyqxmd.com/read/28240653/paraspinal-muscle-passive-stiffness-remodels-in-direct-response-to-spine-stiffness-a-study-using-the-ent1-deficient-mouse
#16
Kelsey Y Gsell, Derek P Zwambag, Dale E Fournier, Cheryle A Séguin, Stephen H M Brown
STUDY DESIGN: Basic science study of the relationship between the structural properties of the spine and its surrounding musculature. OBJECTIVE: To determine if an increase in spine stiffness causes an inverse compensatory change in the passive stiffness of the adjacent paraspinal muscles. SUMMARY OF BACKGROUND DATA: Intervertebral disc degeneration causes an increase in multifidus passive stiffness; this was hypothesized to compensate for a decrease in spine stiffness associated with disc degeneration...
February 24, 2017: Spine
https://www.readbyqxmd.com/read/28222180/pre-existing-neutralizing-antibody-mitigates-b-cell-dysregulation-and-enhances-the-env-specific-antibody-response-in-shiv-infected-rhesus-macaques
#17
Juan Pablo Jaworski, Peter Bryk, Zachary Brower, Bo Zheng, Ann J Hessell, Alexander F Rosenberg, Tong Tong Wu, Ignacio Sanz, Michael C Keefer, Nancy L Haigwood, James J Kobie
Our central hypothesis is that protection against HIV infection will be powerfully influenced by the magnitude and quality of the B cell response. Although sterilizing immunity, mediated by pre-formed abundant and potent antibodies is the ultimate goal for B cell-targeted HIV vaccine strategies, scenarios that fall short of this may still confer beneficial defenses against viremia and disease progression. We evaluated the impact of sub-sterilizing pre-existing neutralizing antibody on the B cell response to SHIV infection...
2017: PloS One
https://www.readbyqxmd.com/read/28214974/interaction-of-scots-pine-defensin-with-model-membrane-by-coarse-grained-molecular-dynamics
#18
Elena Ermakova, Yuriy Zuev
Plant defensins are a part of the innate immune system of plants that acts against a broad range of pathogens. Many plant defensins, including pine defensins, show strong antifungal activity that is associated with their ability to penetrate into the fungal cell membrane. However, the exact molecular mechanism of their action remains poorly defined. To obtain insight into the mechanism of protein-membrane interaction, we applied a coarse-grained molecular dynamics simulation to study the interaction of pine defensin with two model membranes: the first consisted of zwitterion-neutral POPC molecules and the second was composed of combined anionic POPG and POPC...
February 18, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28207656/biomechanical-evaluation-of-transpedicular-nucleotomy-with-intact-annulus-fibrosus
#19
Fabrizio Russo, Robert A Hartman, Kevin M Bell, Nam Vo, Gwendolyn A Sowa, James D Kang, Gianluca Vadalà, Vincenzo Denaro
STUDY DESIGN: Biomechanical testing of partially nucleotomized ovine cadaveric spines. OBJECTIVE: To explore how the nucleus pulposus (NP) affects the biomechanical behavior of the intervertebral disc (IVD) by performing a partial nucleotomy via the transpedicular approach. SUMMARY OF BACKGROUND DATA: Mechanical loading represents a crucial part of IVD homeostasis. However, traditional regenerative strategies require violation of the annulus fibrosus (AF) resulting in significant alteration of joint mechanics...
February 15, 2017: Spine
https://www.readbyqxmd.com/read/28190488/effect-of-a-titanium-cage-as-a-stand-alone-device-on-biomechanical-stability-in-the-lumbosacral-spine-of-canine-cadavers
#20
M Teunissen, A J van der Veen, T H Smit, M A Tryfonidou, B P Meij
Degenerative lumbosacral stenosis is a common disease in dogs characterised by intervertebral disc herniation, loss of disc height and stenosis. Decompressive dorsal laminectomy and partial discectomy can cause spinal instability and worsen foraminal stenosis. Pedicle screw and rod fixation (PSRF) with an intervertebral body cage allows for distraction and restoration of disc height and restores foraminal apertures. The aim of this study was to evaluate the ex vivo biomechanical properties of a titanium intervertebral cage alone and in combination with PSRF in the lumbosacral spine of dogs...
February 2017: Veterinary Journal
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