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https://www.readbyqxmd.com/read/29152314/seroprevalence-of-west-nile-virus-in-horses-in-different-moroccan-regions
#1
Abdennasser Benjelloun, Mehdi El Harrak, Paolo Calistri, Chafiqa Loutfi, Hafsa Kabbaj, Annamaria Conte, Carla Ippoliti, Maria Luisa Danzetta, Bouchra Belkadi
West Nile virus-associated disease is one of the most widespread vector-borne diseases in the world. In Morocco, the first cases were reported in horses in 1996 and the disease re-emerged in 2003 and in 2010. The objective of this work was to study the epidemiological situation of WNV-associated infection in Morocco, by quantifying the seroprevalence of anti-WNV IgM and IgG antibodies in horses in different bioclimatic regions-zones of Morocco in 2011. During the months of May, June and July 2011, 840 serum samples were collected from horses in four regions characterized by different environmental and climatic features such as altitude, temperature and precipitation...
November 2017: Veterinary Medicine and Science
https://www.readbyqxmd.com/read/29078185/development-and-optimization-of-gas-chromatography-coupled-to-high-resolution-mass-spectrometry-based-method-for-the-sensitive-determination-of-dechlorane-plus-and-related-norbornene-based-flame-retardants-in-food-of-animal-origin
#2
Jekaterina Rjabova, Arturs Viksna, Dzintars Zacs
An analytical method has been developed for the trace determination of 10 dechlorane-related compounds (DRCs) in food products by gas chromatography - high resolution mass spectrometry (GC-HRMS). The highest sensitivity of this instrumental analysis method was achieved by selection of the GC column type, optimization of the heated zones within the instrument, and adjusting the electron impact energy. The efficiency of solid phase extraction as clean-up procedure was also optimized. Two different types of cartridges - neutral silica gel and Florisil(®), as well as seven organic solvents or their mixtures - n-hexane, cyclohexane, acetone, toluene, n-hexane/ethyl acetate, n-hexane/acetone, and n-hexane/dichloromethane - were evaluated...
October 19, 2017: Chemosphere
https://www.readbyqxmd.com/read/29054117/assessing-global-range-expansion-in-a-cryptic-species-complex-insights-from-the-red-seaweed-genus-asparagopsis-florideophyceae
#3
Marianela Zanolla, María Altamirano, Raquel Carmona, Julio De la Rosa, Virginia Souza-Egipsy, Alison Sherwood, Konstantinos Tsiamis, Ana Márcia Barbosa, Antonio Román Muñoz, Nikos Andreakis
The mitochondrial genetic diversity, distribution and invasive potential of multiple cryptic operational taxonomic units (OTUs) of the red invasive seaweed Asparagopsis were assessed by studying introduced Mediterranean and Hawaiian populations. Invasive behavior of each Asparagopsis OTU was inferred from phylogeographic reconstructions, past historical demographic dynamics, recent range expansion assessments and future distributional predictions obtained from demographic models. Genealogical networks resolved Asparagopsis gametophytes and tetrasporophytes into four A...
October 20, 2017: Journal of Phycology
https://www.readbyqxmd.com/read/29037407/serum-concentrations-of-free-amino-acids-in-growing-pigs-exposed-to-diurnal-heat-stress-fluctuations
#4
Miguel Cervantes, Nely Ibarra, Nydia Vásquez, Francisco Reyes, Ernesto Avelar, Salvador Espinoza, Adriana Morales
In areas where the ambient temperature (AT) is above the thermo neutral (TN) zone of pigs, significant changes within a 24-h period occur, differently affecting the availability of amino acids (AA) within the same day. An experiment was conducted to analyze the serum concentrations (SC) of free AA in pigs exposed to diurnal variations in AT. Six pigs (27.1 ±1.3kg body weight) implanted with a thermometer to register the body temperature (BT) at 15-min intervals were used. Blood samples were collected on the last 3 d of the 14-d study, at 0700h (lowest AT), 1200h (mild HS), and 1600h (severe HS)...
October 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/29019693/dynamic-structuration-of-physical-chitosan-hydrogels
#5
Nicolas Sereni, Alin Enache, Guillaume Sudre, Alexandra Montembault, Cyrille Rochas, Philippe Durand, Marie-Hélène Perrard, Grigore Bozga, Jean-Pierre Puaux, Thierry Delair, Laurent David
We studied the microstructure of physical chitosan hydrogels formed by the neutralization of chitosan aqueous solutions highlighting the structural gradients within thick gels (up to a thickness of 16 mm). We explored a high polymer concentrations range (Cp ≥ 1.0% w/w) with different molar masses of chitosan and different concentrations of the coagulation agent. The effect of these processing parameters on the morphology was evaluated mainly through small-angle light scattering (SALS) measurements and confocal laser scanning microscopy (CLSM) observations...
October 24, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28988711/in%C3%A2-vivo-selection-of-a-computationally-designed-schema-aav-library-yields-a-novel-variant-for-infection-of-adult-neural-stem-cells-in-the-svz
#6
David S Ojala, Sabrina Sun, Jorge L Santiago-Ortiz, Mikhail G Shapiro, Philip A Romero, David V Schaffer
Directed evolution continues to expand the capabilities of complex biomolecules for a range of applications, such as adeno-associated virus vectors for gene therapy; however, advances in library design and selection strategies are key to develop variants that overcome barriers to clinical translation. To address this need, we applied structure-guided SCHEMA recombination of the multimeric adeno-associated virus (AAV) capsid to generate a highly diversified chimeric library with minimal structural disruption...
September 8, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28987006/effects-of-assortative-mate-choice-on-the-genomic-and-morphological-structure-of-a-hybrid-zone-between-two-bird-subspecies
#7
Georgy A Semenov, Elizabeth S C Scordato, David R Khaydarov, Chris C R Smith, Nolan C Kane, Rebecca J Safran
Phenotypic differentiation plays an important role in the formation and maintenance of reproductive barriers. In some cases, variation in a few key aspects of phenotype can promote and maintain divergence; hence, the identification of these traits and their associations with patterns of genomic divergence is crucial for understanding the patterns and processes of population differentiation. We studied hybridization between the alba and personata subspecies of the white wagtail (Motacilla alba), and quantified divergence and introgression of multiple morphological traits and 19,437 SNP loci on a 3,000 km transect...
October 7, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28982176/affective-vocalizations-influence-body-ownership-as-measured-in-the-rubber-hand-illusion
#8
Tahnée Engelen, Rebecca Watson, Francesco Pavani, Beatrice de Gelder
Emotional signals, like threatening sounds, automatically ready the perceiver to prepare an appropriate defense behavior. Conjecturing that this would manifest itself in extending the safety zone around the body we used the rubber hand illusion (RHI) to test this prediction. The RHI is a perceptual illusion in which body ownership is manipulated by synchronously stroking a rubber hand and real hand occluded from view. Many factors, both internal and external, have been shown to influence the strength of the illusion, yet the effect of emotion perception on body ownership remains unexplored...
2017: PloS One
https://www.readbyqxmd.com/read/28978569/marginal-zone-b-cells-are-critical-to-factor-viii-inhibitor-formation-in-mice-with-hemophilia-a
#9
Patricia E Zerra, Courtney Cox, W Hunter Baldwin, Seema R Patel, Connie M Arthur, Pete Lollar, Shannon L Meeks, Sean R Stowell
While factor VIII (FVIII) replacement therapy can be life saving for patients with hemophilia A, neutralizing alloantibodies to FVIII, known as inhibitors, develop in a significant number of patients and actively block FVIII activity, making bleeding difficult to control and prevent. Although a variety of downstream immune factors likely regulate inhibitor formation, the identification and subsequent targeting of key initiators in inhibitor development may provide an attractive approach to prevent inhibitor formation before amplification of the FVIII immune response occurs...
October 4, 2017: Blood
https://www.readbyqxmd.com/read/28948549/delayed-effects-of-neonatal-administration-of-non-opioid-analog-of-leu-enkephalin-on-cerebral-consequences-of-antenal-hypoxia
#10
A A Simankova, E N Sazonova, O A Lebed'ko
In 60-day-old male rats after antenatal hypoxia, the body weight and the absolute weight of the cerebral hemispheres were significantly lower than in the progeny of intact animals. Analysis of brain sections stained with silver nitrate revealed reduced number of nucleoli in neocortical layer II and hippocampal CA1 neurons and smaller area of neuronal nuclei in neocortical layer V and total area of nucleoli in neurons of all studied zones. The animals demonstrated increased locomotor activity in the elevated plus-maze test...
September 25, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28945799/the-riddle-of-mitochondrial-alkaline-neutral-invertases-a-novel-arabidopsis-isoform-mainly-present-in-reproductive-tissues-and-involved-in-root-ros-production
#11
Marina E Battaglia, María Victoria Martin, Leandra Lechner, Giselle M A Martínez-Noël, Graciela L Salerno
Alkaline/neutral invertases (A/N-Inv), glucosidases that irreversibly hydrolyze sucrose into glucose and fructose, play significant roles in plant growth, development, and stress adaptation. They occur as multiple isoforms located in the cytosol or organelles. In Arabidopsis thaliana, two mitochondrial A/N-Inv genes (A/N-InvA and A/N-InvC) have already been investigated. In this study, we functionally characterized A/N-InvH, a third Arabidopsis gene coding for a mitochondrial-targeted protein. The phenotypic analysis of knockout mutant plants (invh) showed a severely reduced shoot growth, while root development was not affected...
2017: PloS One
https://www.readbyqxmd.com/read/28934173/using-upper-extremity-skin-temperatures-to-assess-thermal-comfort-in-office-buildings-in-changsha-china
#12
Zhibin Wu, Nianping Li, Haijiao Cui, Jinqing Peng, Haowen Chen, Penglong Liu
Existing thermal comfort field studies are mainly focused on the relationship between the indoor physical environment and the thermal comfort. In numerous chamber experiments, physiological parameters were adopted to assess thermal comfort, but the experiments' conclusions may not represent a realistic thermal environment due to the highly controlled thermal environment and few occupants. This paper focuses on determining the relationships between upper extremity skin temperatures (i.e., finger, wrist, hand and forearm) and the indoor thermal comfort...
September 21, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28931551/emc10-endoplasmic-reticulum-membrane-protein-complex-subunit-10-is-a-bone-marrow-derived-angiogenic-growth-factor-promoting-tissue-repair-after-myocardial-infarction
#13
Marc R Reboll, Mortimer Korf-Klingebiel, Stefanie Klede, Felix Polten, Eva Brinkmann, Ines Reimann, Hans-Joachim Schönfeld, Maria Bobadilla, Jan Faix, George Kensah, Ina Gruh, Michael Klintschar, Matthias Gaestel, Hans W Niessen, Andreas Pich, Johann Bauersachs, Joseph A Gogos, Yong Wang, Kai C Wollert
BACKGROUND: Clinical trials of bone marrow cell-based therapies after acute myocardial infarction (MI) have produced mostly neutral results. Treatment with specific bone marrow cell-derived secreted proteins may provide an alternative biological approach to improving tissue repair and heart function after MI. We recently performed a bioinformatic secretome analysis in bone marrow cells from patients with acute MI and discovered a poorly characterized secreted protein, EMC10 (endoplasmic reticulum membrane protein complex subunit 10), showing activity in an angiogenic screen...
November 7, 2017: Circulation
https://www.readbyqxmd.com/read/28922530/a-space-charge-treatment-of-the-increased-concentration-of-reactive-species-at-the-surface-of-a-ceria-solid-solution
#14
Alexander F Zurhelle, Xiaorui Tong, Andreas Klein, David S Mebane, Roger A De Souza
A space-charge theory applicable to concentrated solid solutions (Poisson-Cahn theory) was applied to describe quantitatively as a function of temperature and oxygen partial pressure published data obtained by in situ X-ray photoelectron spectroscopy (XPS) for the concentration of Ce(3+) (the reactive species) at the surface of the oxide catalyst Ce0.8 Sm0.2 O1.9 . In contrast to previous theoretical treatments, these calculations clearly indicate that the surface is positively charged and compensated by an attendant negative space-charge zone...
September 18, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28921977/photoinduced-bimolecular-electron-transfer-in-ionic-liquids
#15
Boning Wu, Mark Maroncelli, Edward W Castner
The present work seeks to better understand the role of solute diffusion and solvation dynamics on bimolecular electron transfer in ionic liquids (ILs). Steady-state and time-resolved measurements of the reductive fluorescence quenching of five fluorophores ("F") by six quenchers ("Q"; electron donors) are reported in acetonitrile and two ionic liquids, 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)amide and trihexyl(tetradecyl)phosphonium bis(trifluoromethylsulfonyl)amide. Data were collected on 66 different F-Q-solvent systems, which span a 2...
October 5, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28904636/the-orthopaedic-management-of-lower-limb-deformity-in-hypophosphataemic-rickets
#16
A Horn, J Wright, D Bockenhauer, W Van't Hoff, D M Eastwood
BACKGROUND: Many patients with X-linked hypophosphataemic rickets (X-LHPR) demonstrate significant lower limb deformity despite optimal medical management. This study evaluates the use of guided growth by means of hemi-epiphysiodesis to address coronal plane deformity in the skeletally immature child. METHODS: Since 2005, 24 patients with X-LHPR have been referred to our orthopaedic unit for evaluation. All patients had standardised long leg radiographs that were analysed sequentially before and after surgery if any was performed...
August 1, 2017: Journal of Children's Orthopaedics
https://www.readbyqxmd.com/read/28902471/narrow-hybrid-zones-in-spite-of-very-low-population-differentiation-in-neutral-markers-in-an-island-bird-species-complex
#17
Boris Delahaie, Josselin Cornuault, Charline Masson, Joris A M Bertrand, Yann X C Bourgeois, Borja Milá, Christophe Thébaud
Patterns of phenotypic and genic frequencies across hybrid zones provide insight into the origin and evolution of reproductive isolation. The Reunion grey white-eye, Zosterops borbonicus, exhibits parapatrically distributed plumage colour forms across the lowlands of the small volcanic island of Reunion (Mascarene archipelago). These forms meet and hybridise in regions that are natural barriers to dispersal (rivers, lava fields). Here, we investigated the relationship among patterns of differentiation at neutral genetic (microsatellite) markers, phenotypic traits (morphology and plumage colour) and niche characteristics across three independent hybrid zones...
September 13, 2017: Journal of Evolutionary Biology
https://www.readbyqxmd.com/read/28898815/biomechanical-evaluation-of-different-surgical-procedures-in-single-level-transforaminal-lumbar-interbody-fusion-in-vitro
#18
Yuanwu Cao, Fubing Liu, Shengcheng Wan, Yun Liang, Chun Jiang, Zhenzhou Feng, Xiaoxing Jiang, Zixian Chen
BACKGROUNDS: A variety of improved surgical methods were adopted in the transforaminal lumbar interbody fusion. A mechanical stability provides an ideal environment for the formation of a fusion mass and is the basis of their good outcomes. The object of this study is to evaluate the initial similarities and differences of four commonly-used posterior surgical procedures biomechanically. METHODS: Biomechanical testing was performed at L3-4 motion segment in 6 fresh-frozen human cadaveric lumbar spines (L2-L5), including the following sequentially tested configurations: 1) intact motion segment; 2) bilateral pedicle screw fixation; 3) unilateral pedicle screw fixation; 4) unilateral pedicle screw plus contralateral translaminar facet joint screw fixation according to the Magerl technique; and 5) bilateral pedicle screw fixation with bilateral facetectomies...
September 5, 2017: Clinical Biomechanics
https://www.readbyqxmd.com/read/28895029/chemical-characteristics-of-soluble-aerosols-over-the-central-himalayas-insights-into-spatiotemporal-variations-and-sources
#19
Lekhendra Tripathee, Shichang Kang, Dipesh Rupakheti, Zhiyuan Cong, Qianggong Zhang, Jie Huang
In order to investigate the spatial and temporal variations of aerosols and its soluble chemical compositions of the data gap zone in the central Himalayan region, aerosol samples were collected at four sites. The sampling location were characterized by four different categories, such as urban (Bode), semi-urban site in the northern Indo-Gangetic Plain (Lumbini), rural (Dhunche), and semiarid rural (Jomsom). A total of 230 aerosol samples were collected from four representative sites for a yearlong period and analyzed for water-soluble inorganic ions (WSIIs)...
September 12, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28890702/a-pilot-study-of-the-effects-of-mindfulness-based-stress-reduction-on-post-traumatic-stress-disorder-symptoms-and-brain-response-to-traumatic-reminders-of-combat-in-operation-enduring-freedom-operation-iraqi-freedom-combat-veterans-with-post-traumatic-stress
#20
James Douglas Bremner, Sanskriti Mishra, Carolina Campanella, Majid Shah, Nicole Kasher, Sarah Evans, Negar Fani, Amit Jasvant Shah, Collin Reiff, Lori L Davis, Viola Vaccarino, James Carmody
OBJECTIVE: Brain imaging studies in patients with post-traumatic stress disorder (PTSD) have implicated a circuitry of brain regions including the medial prefrontal cortex, amygdala, hippocampus, parietal cortex, and insula. Pharmacological treatment studies have shown a reversal of medial prefrontal deficits in response to traumatic reminders. Mindfulness-based stress reduction (MBSR) is a promising non-pharmacologic approach to the treatment of anxiety and pain disorders. The purpose of this study was to assess the effects of MBSR on PTSD symptoms and brain response to traumatic reminders measured with positron-emission tomography (PET) in Operation Enduring Freedom/Operation Iraqi Freedom (OEF/OIF) combat veterans with PTSD...
2017: Frontiers in Psychiatry
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