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https://www.readbyqxmd.com/read/28814299/biological-function-in-the-twilight-zone-of-sequence-conservation
#1
REVIEW
Chris P Ponting
Strong DNA conservation among divergent species is an indicator of enduring functionality. With weaker sequence conservation we enter a vast 'twilight zone' in which sequence subject to transient or lower constraint cannot be distinguished easily from neutrally evolving, non-functional sequence. Twilight zone functional sequence is illuminated instead by principles of selective constraint and positive selection using genomic data acquired from within a species' population. Application of these principles reveals that despite being biochemically active, most twilight zone sequence is not functional...
August 16, 2017: BMC Biology
https://www.readbyqxmd.com/read/28754482/patients-with-compromised-oral-health-related-quality-of-life-experienced-an-improvement-when-wearing-complete-dentures-fabricated-conventionally-or-using-the-neutral-zone-concept
#2
Romina Brignardello-Petersen
No abstract text is available yet for this article.
July 17, 2017: Journal of the American Dental Association
https://www.readbyqxmd.com/read/28745028/chemical-communication-sexual-selection-and-introgression-in-wall-lizards
#3
Hannah E A MacGregor, Rachel A M Lewandowsky, Patrizia d'Ettorre, Chloé Leroy, Noel W Davies, Geoffrey M While, Tobias Uller
Divergence in communication systems should influence the likelihood that individuals from different lineages interbreed, and consequently shape the direction and rate of hybridization. Here, we studied the role of chemical communication in hybridization, and its contribution to asymmetric and sexually selected introgression, between two lineages of the common wall lizard (Podarcis muralis). Males of the two lineages differed in the chemical composition of their femoral secretions. Chemical profiles provided information regarding male secondary sexual characters, but the associations were variable and inconsistent between lineages...
July 26, 2017: Evolution; International Journal of Organic Evolution
https://www.readbyqxmd.com/read/28744904/do-mandibular-complete-dentures-made-using-a-neutral-zone-technique-improve-speech-a-pilot-study
#4
Peter Rehmann, Anna Katharina Künkel, Daniel Weber, Ulrich Lotzmann, Bernd Wöstmann
OBJECTIVE: The aim of this study was to evaluate speech sound production (SSP) in older edentulous patients provided with two different types of mandibular complete dentures (MCDs; conventional vs neutral zone). BACKGROUND: Regarding the fact that complete dentures (CDs) affect SSP, it is unknown whether the set-up of the artificial teeth resulting from the neutral zone technique leads to a negative impact on SSP. MATERIALS AND METHODS: For 21 participants, a conventional MCD and a MCD using a modified neutral zone technique (neutral zone dentures) were fabricated...
July 26, 2017: Gerodontology
https://www.readbyqxmd.com/read/28735027/translation-of-an-injectable-triple-interpenetrating-network-hydrogel-for-intervertebral-disc-regeneration-in-a-goat-model
#5
Sarah E Gullbrand, Thomas P Schaer, Prateek Agarwal, Justin R Bendigo, George R Dodge, Weiliam Chen, Dawn M Elliott, Robert L Mauck, Neil R Malhotra, Lachlan J Smith
Degeneration of the intervertebral discs is a progressive cascade of cellular, compositional and structural changes that is frequently associated with low back pain. As the first signs of disc degeneration typically arise in the disc's central nucleus pulposus (NP), augmentation of the NP via hydrogel injection represents a promising strategy to treat early to mid-stage degeneration. The purpose of this study was to establish the translational feasibility of a triple interpenetrating network hydrogel composed of dextran, chitosan, and teleostean (DCT) for augmentation of the degenerative NP in a preclinical goat model...
July 19, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28734500/a-new-online-delay-estimation-based-robust-adaptive-stabilizer-for-multi-input-neutral-systems-with-unknown-actuator-nonlinearities
#6
A H Tahoun
This paper studies the problem of actuator-nonlinearities compensation in the multi-input uncertain neutral systems. The neutral systems with different unknown time-varying delays, unmodeled dynamics, nonlinear perturbations and disturbances are considered. A new methodology based on the online estimation of the delays to compensate for the effects generated by dead-zone and saturation, acting in series on a system's input are presented. The online estimations of the unknown delays are accomplished by adaptive laws that guarantee the exponential stabilities of the estimated delays...
July 19, 2017: ISA Transactions
https://www.readbyqxmd.com/read/28724263/mutagenesis-and-characterization-of-a-bacillus-amyloliquefaciens-strain-for-cinnamomum-camphora-seed-kernel-oil-extraction-by-aqueous-enzymatic-method
#7
Cheng Zeng, Rongbin Zhao, Maomao Ma, Zheling Zeng, Deming Gong
The purpose of the present study was to increase the proteinase activity of the strain NCU116 by combining ultraviolet irradiation and N-methyl-N'-nitro-N-nitroso guanidine treatment, in order to enhance the efficiency of Cinnamomum camphora seed kernel oil (CCSKO) extraction by aqueous enzymatic method (AEM). The mutated strain, designated as NCU116-1, was screened out by the ratio of hydrolytic zone diameter to colony diameter on skim milk plate. The proteinase activity (9116.1 U/ml) of NCU116-1 was increased by 31...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28720338/complete-denture-fabrication-using-piezography-and-cad-cam-a-clinical-report
#8
Chikahiro Ohkubo, Hidemasa Shimpo, Ai Tokue, Eun-Jin Park, Tae Hyung Kim
To provide maxillary and mandibular complete dentures for a patient with severe ridge resorption, the denture space was recorded using the piezography technique. After the piezographic space was scanned, a virtual tooth arrangement and festooning were performed within the space using computer-aided design software. The denture bases were milled from a polymethylmethacrylate resin block using computer-aided manufacturing, and commercially available denture teeth were bonded with resin adhesive. Using the piezography technique described, physiologically appropriate complete dentures were fabricated based on the neutral zone concept...
July 15, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28701033/ionic-strength-differentially-affects-the-bioavailability-of-neutral-and-negatively-charged-inorganic-hg-complexes
#9
Benjamin Robert Stenzler, Aaron Hinz, Matti O Ruuskanen, Alexandre J Poulain
Mercury (Hg) bioavailability to bacteria in marine systems is the first step towards its bioamplification in food webs. These systems exhibit high salinity and ionic strength which will both alter Hg speciation and properties of the bacteria cell walls. The role of Hg speciation on Hg bioavailability in marine systems has not been teased apart from that of ionic strength on cell wall properties, however. We developed and optimized a whole-cell Hg bioreporter capable of functioning under aerobic and anaerobic conditions and exhibiting no physiological limitations of signal production to changes in ionic strength...
July 13, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28699878/genetically-stable-infectious-schmallenberg-virus-persists-in-foetal-envelopes-of-pregnant-ewes
#10
Antoine Poskin, Ludovic Martinelle, Yves Van der Stede, Claude Saegerman, Brigitte Cay, Nick De Regge
Schmallenberg virus (SBV) is a recently emerged vector-borne virus, inducing congenital defects in bovines, ovines and caprines. Here we have shown that infectious SBV is capable of persisting until the moment of birth in the foetal envelopes of ewes infected with SBV-infectious serum at day 45 (1/5 positive) and 60 (4/6 positive) of gestation. This persistence of at least 100 days is a new aspect of the SBV pathogenesis that could help to explain how SBV overwinters the cold season in temperate climate zones...
July 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28697202/a-randomized-crossover-trial-comparing-patient-preference-for-mandibular-complete-dentures-made-with-two-different-techniques-a-short-term-follow-up
#11
Greta Avm Geerts
PURPOSE: The objective of this trial was to compare short-term patient preference for two mandibular complete denture (CD) fabrication techniques: neutral zone (NZ) and conventional (CV). MATERIALS AND METHODS: A prospective, within-subject, randomized crossover trial was performed on a sample of 37 edentulous patients with complete denture experience. Two new sets of CDs were made for each patient, one set with a CV and one with a NZ mandibular denture. After consecutively wearing each set for at least 2 months after the last adjustment visit, patients indicated their preference (CV, NZ, or none)...
July 2017: International Journal of Prosthodontics
https://www.readbyqxmd.com/read/28690335/spatially-resolved-images-of-reactive-ions-in-the-orion-bar-%C3%A2-%C3%A2
#12
Javier R Goicoechea, Sara Cuadrado, Jérôme Pety, Emeric Bron, John H Black, José Cernicharo, Edwige Chapillon, Asunción Fuente, Maryvonne Gerin
We report high angular resolution (4.9″×3.0″) images of reactive ions SH(+), HOC(+), and SO(+) toward the Orion Bar photodissociation region (PDR). We used ALMA-ACA to map several rotational lines at 0.8 mm, complemented with multi-line observations obtained with the IRAM 30 m telescope. The SH(+) and HOC(+) emission is restricted to a narrow layer of 2″- to 10″-width (≈800 to 4000 AU depending on the assumed PDR geometry) that follows the vibrationally excited [Formula: see text] emission. Both ions efficiently form very close to the H/H2 transition zone, at a depth of Av≲1 mag into the neutral cloud, where abundant C(+), S(+), and [Formula: see text] coexist...
May 2017: Astronomy and Astrophysics
https://www.readbyqxmd.com/read/28683759/intravenous-neutralization-of-vascular-endothelial-growth-factor-reduces-vascular-function-permeability-of-the-ovary-and-prevents-development-of-ohss-like-symptoms-in-rhesus-monkeys
#13
C V Bishop, D M Lee, O D Slayden, X Li
BACKGROUND: Ovarian hyperstimulation syndrome (OHSS) is a disorder associated with elevated serum VEGFA following chorionic gonadotropin (hCG) exposure in controlled ovarian stimulation (COS) cycles in women. In this study, we tested the effect of intravenous VEGFA neutralization on OHSS-like symptoms and vascular function in rhesus macaques during COS cycles. METHODS: Monkeys (n = 8) were treated with 3 COS protocols and assigned randomly to groups as follows: 1) COS alone (Control, n = 5); 2) COS + VEGF mAb Avastin 19 ± 5 h before hCG (Avastin pre-hCG; n = 6); 3) COS + Avastin 3-4 days post-hCG (Avastin post-hCG; n = 4); 4) COS + Simulated Early Pregnancy (SEP n = 3); or 5) COS + SEP + Avastin (SEP + Avastin n = 3)...
July 6, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28682110/is-intervertebral-disc-degeneration-related-to-segmental-instability-an-evaluation-with-two-different-grading-systems-based-on-clinical-imaging
#14
David Volkheimer, Fabio Galbusera, Christian Liebsch, Sabine Schlegel, Friederike Rohlmann, Sebastian Kleiner, Hans-Joachim Wilke
Background Several in vitro studies investigated how degeneration affects spinal motion. However, no consensus has emerged from these studies. Purpose To investigate how degeneration grading systems influence the kinematic output of spinal specimens. Material and Methods Flexibility testing was performed with ten human T12-S1 specimens. Degeneration was graded using two different classifications, one based on X-ray and the other one on magnetic resonance imaging (MRI). Intersegmental rotation (expressed by range of motion [ROM] and neutral zone [NZ]) was determined in all principal motion directions...
January 1, 2017: Acta Radiologica
https://www.readbyqxmd.com/read/28679119/biodegradable-plastic-bags-on-the-seafloor-a-future-threat-for-seagrass-meadows
#15
Elena Balestri, Virginia Menicagli, Flavia Vallerini, Claudio Lardicci
Marine plastic litter is a global concern. Carrier bags manufactured from non-biodegradable polymers constitute a large component of this litter. Because of their adverse impact on marine life, non-biodegradable bags have recently been replaced by biodegradable ones. However, growing evidence shows that these latter are not readily degradable in marine sediments and can alter benthic assemblages. The potential impact of biodegradable bags on seagrasses inhabiting sandy bottoms, which are the most widespread and productive ecosystems of the coastal zones, has been ignored...
July 2, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28666766/toxicogenomic-responses-of-human-alveolar-epithelial-cells-to-tungsten-boride-nanoparticles
#16
Hasan Türkez, Mehmet Enes Arslan, Erdal Sönmez, Abdulgani Tatar, Metin Açikyildiz, Fatime Geyikoğlu
During the recent years, microarray analysis of gene expression has become an inevitable tool for exploring toxicity of drugs and other chemicals on biological systems. Therefore, toxicogenomics is considered as a fruitful area for searching cellular pathways and mechanisms including cancer, immunological diseases, environmental responses, gene-gene interactions and chemical toxicity. In this work, we examined toxic effects of Tungsten Borides NPs on gene expression profiling of the human lung alveolar epithelial cells (HPAEpiC)...
June 27, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28664249/hydrogeochemistry-study-and-groundwater-quality-assessment-in-the-north-of-isfahan-iran
#17
Ali Rezaei, Hossein Hassani
This study presents the groundwater quality assessment in the north of Isfahan, Iran. In the study area, assessment and measurement of groundwater hydrochemical parameters such as pH, total dissolved solids (TDS), electrical conductivity (EC), sodium absorption ratio (SAR), total hardness, major cations (K(+), Na(+), Ca(2+) and Mg(2+)) and major anions (Cl(-), [Formula: see text] and [Formula: see text]) concentrations were performed. Accordingly, the 66 water samples from different locations were collected during April and May 2015...
June 29, 2017: Environmental Geochemistry and Health
https://www.readbyqxmd.com/read/28663394/external-rotation-of-the-femoral-component-increases-asymmetry-of-the-posterior-condyles
#18
M P Bonnin, M Saffarini, L Nover, J van der Maas, C Haeberle, G Hannink, J Victor
AIMS: The morphometry of the distal femur was largely studied to improve bone-implant fit in total knee arthroplasty (TKA), but little is known about the asymmetry of the posterior condyles. This study aimed to investigate the dimensions of the posterior condyles and the influence of externally rotating the femoral component on potential prosthetic overhang or under-coverage. PATIENTS AND METHODS: We analysed the shape of 110 arthritic knees at the time of primary TKA using pre-operative CT scans...
July 2017: Bone & Joint Journal
https://www.readbyqxmd.com/read/28653444/ambient-temperature-mediated-changes-in-hepatic-gene-expression-of-a-mammalian-herbivore-neotoma-lepida
#19
Patrice Kurnath Connors, Jael R Malenke, M Denise Dearing
Herbivores regularly ingest natural toxins produced by plants as a defence against herbivory. Recent work suggests that compound toxicity is exacerbated at higher ambient temperatures. This phenomenon, known as temperature-dependent toxicity (TDT), is the likely result of decreased liver function at warmer temperatures; however, the underlying cause of TDT remains speculative. In the present study, we compared the effects of temperature and dietary plant toxins on differential gene expression in the liver of an herbivorous rodent (Neotoma lepida), using species-specific microarrays...
June 27, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28647236/hydrolysis-of-polyaluminum-chloride-prior-to-coagulation-effects-on-coagulation-behavior-and-implications-for-improving-coagulation-performance
#20
Zhongguo Zhang, Jun Wang, Dan Liu, Jiuyi Li, Xiaolin Wang, Boyu Song, Bing Yue, Kehui Zhao, Yun Song
The effects of polyaluminum chloride (PACl) hydrolysis prior to coagulation on both the coagulation zone and coagulation performance of a kaolin suspension were investigated by a novel jar test named the "reversed coagulation test". The tests showed that PACl hydrolysis prior to coagulation decreased the performance of charge neutralization coagulation in the case of short-time slow mixing (10min; G=15sec(-1)) and increased the optimal dosage for charge neutralization and sweep coagulation. Moreover, the hydrolysis time had insignificant effects on the size and zeta potential of PACl precipitates and the residual turbidity of the raw water...
July 2017: Journal of Environmental Sciences (China)
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