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https://www.readbyqxmd.com/read/28438657/the-thiol-of-human-serum-albumin-acidity-microenvironment-and-mechanistic-insights-on-its-oxidation-to-sulfenic-acid
#1
Jenner Bonanata, Lucía Turell, Laura Antmann, Gerardo Ferrer-Sueta, Santiago Botasini, Eduardo Méndez, Beatriz Alvarez, E Laura Coitiño
Human serum albumin (HSA) has a single reduced cysteine residue, Cys34, whose acidity has been controversial. Three experimental approaches (pH-dependence of reactivity towards hydrogen peroxide, ultraviolet titration and infrared spectroscopy) are used to determine that the pKa value in delipidated HSA is 8.1 ± 0.2 at 37°C and 0.1M ionic strength. Molecular dynamics simulations of HSA in the sub-microsecond timescale show that while sulfur exposure to solvent is limited and fluctuating in the thiol form, it increases in the thiolate, stabilized by a persistent hydrogen-bond (HB) network involving Tyr84 and bridging waters to Asp38 and Gln33 backbone...
April 21, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28437978/tunable-optical-cage-array-generated-by-dammann-vector-beam
#2
Xiaoyu Weng, Luping Du, Peng Shi, Xiaocong Yuan
Optical cages attract considerable attentions recently owing to their potential applications in optical trapping, optical imaging and optical cloaking. However, the generation of tunable optical cage arrays in the transverse plane comes to a great challenge, which restricts the effectiveness of the above applications. In this work, we propose a full polarization-controlled method that optical cage arrays with tunable number and positions in the x-y plane can be generated by a so-called Dammann vector beam (DVB), both under the conditions of high- and low-NA focusing system...
April 17, 2017: Optics Express
https://www.readbyqxmd.com/read/28437330/pedicle-screw-fluid-sign-an-indication-on-magnetic-resonance-imaging-of-a-deep-infection-after-posterior-spinal-instrumentation
#3
Hiroaki Kimura, Jitsuhiko Shikata, Seiichi Odate, Tsunemitsu Soeda
STUDY DESIGN: A single-center case-referent study. OBJECTIVE: To assess whether the "pedicle screw (PS) fluid sign" on magnetic resonance imaging (MRI) can be used to diagnose deep surgical site infection (SSI) after posterior spinal instrumentation (PSI). SUMMARY OF BACKGROUND DATA: MRI is a useful tool for the early diagnosis of a deep SSI. However, the diagnosis is frequently difficult with feverish patients with clear wounds after PSI because of artifacts from the metallic implants...
May 2017: Clinical Spine Surgery
https://www.readbyqxmd.com/read/28433848/instrumented-circumferential-fusion-in-two-stages-for-instable-lumbar-fracture-long-term-results-of-a-series-of-74-patients-on-sagittal-balance-and-functional-outcomes
#4
Mourad Ould-Slimane, Camille Damade, Guillaume Lonjon, André Gilibert, Jérôme Cochereau, Rémi Gauthé, Nicolas Lonjon
AIM: To report the radiological and functional results of a multicentre, prospective case series of patients with comminuted lumbar fractures treated with two-stage circumferential arthrodesis. METHODS: A multicentre, prospective case series of 74 patients with comminuted lumbar fractures was analyzed. The strategy entailed initial posterior osteosynthesis then physical replacement with an expandable titanium cage filled with autologous bone, via retroperitoneal lumbotomy...
April 19, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28431093/interactions-between-cassava-mosaic-geminiviruses-and-their-vector-bemisia-tabaci-hemiptera-aleyrodidae
#5
Simon B Boni, Costancia P Rugumamu, Dan Gerling, K Sagary Nokoe, James P Legg
The sweetpotato whitefly, Bemisia tabaci (Gennadius) is the vector of the cassava mosaic geminiviruses (CMGs) that cause cassava mosaic disease (CMD). Synergistic interactions between B. tabaci and CMGs have been hypothesized as a cause of whitefly "super-abundance," which has been a key factor behind the spread of the severe CMD pandemic through East and Central Africa. The current study investigated this hypothesis by conducting experiments with CMD-susceptible cassava varieties infected with different CMGs in both the north-western Lake Zone region (pandemic affected) and the eastern Coast Zone where CMD is less severe...
April 18, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28428145/prior-treadmill-exercise-promotes-resilience-to-vicarious-trauma-in-rats
#6
Camila Kochi, Hesong Liu, Safiyya Zaidi, Fatin Atrooz, Phoebe Dantoin, Samina Salim
BACKGROUND: Post-traumatic stress disorder (PTSD) is a serious psychological condition, which can develop both from physically experiencing and also from witnessing traumatic events. There is evidence that physical exercise can have a positive impact on the symptoms of PTSD. Relevant to this, in our previous pre-clinical work, beneficial effects of treadmill exercise were reported on PTSD-like behaviors in a social defeat paradigm, a rat model of direct physical trauma. However, the role of exercise on vicariously acquired PTSD-like phenotype was not examined...
April 17, 2017: Progress in Neuro-psychopharmacology & Biological Psychiatry
https://www.readbyqxmd.com/read/28422979/correlation-of-egfr-or-kras-mutation-status-with-18f-fdg-uptake-on-pet-ct-scan-in-lung-adenocarcinoma
#7
Kazuya Takamochi, Kaoru Mogushi, Hideya Kawaji, Kota Imashimizu, Mariko Fukui, Shiaki Oh, Masayoshi Itoh, Yoshihide Hayashizaki, Weijey Ko, Masao Akeboshi, Kenji Suzuki
BACKGROUND: 18F-fluoro-2-deoxy-glucose (18F-FDG) positron emission tomography (PET) is a functional imaging modality based on glucose metabolism. The correlation between EGFR or KRAS mutation status and the standardized uptake value (SUV) of 18F-FDG PET scanning has not been fully elucidated. METHODS: Correlations between EGFR or KRAS mutation status and clinicopathological factors including SUVmax were statistically analyzed in 734 surgically resected lung adenocarcinoma patients...
2017: PloS One
https://www.readbyqxmd.com/read/28413431/asian-house-rats-may-facilitate-their-invasive-success-through-suppressing-brown-rats-in-chronic-interaction
#8
Hong-Ling Guo, Hua-Jing Teng, Jin-Hua Zhang, Jian-Xu Zhang, Yao-Hua Zhang
BACKGROUND: The Asian house rat (Rattus tanezumi) and the brown rat (Rattus norvegicus) are closely related species and are partially sympatric in southern China. Over the past 20 years, R. tanezumi has significantly expanded northward in China and partially replaced the native brown rat subspecies, R. n. humiliatus. Although invasive species are often more aggressive than native species, we did not observe interspecific physical aggression between R. tanezumi and R. n. humiliatus. Here, we focused on whether or not R...
2017: Frontiers in Zoology
https://www.readbyqxmd.com/read/28397041/effects-of-nourishing-liver-and-kidney-acupuncture-therapy-on-expression-of-brain-derived-neurotrophic-factor-and-synaptophysin-after-cerebral-ischemia-reperfusion-in-rats
#9
Wen-Guang Xia, Chan-Juan Zheng, Xuan Zhang, Juan Wang
The aim of the present study was to investigate the effect of "nourishing liver and kidney" acupuncture therapy on motor and cognitive deficits, and the underlying mechanism following cerebral ischemia-reperfusion (I/R) via increasing the expression of brain derived neurotrophic factor (BDNF) and synaptophysin (SYN) in the hippocampus. Healthy adult male SD rats were randomly divided into sham operation group (n=51), model group (n=51), acupuncture group (n=51) and acupuncture control group (n=51). The middle cerebral I/R model was established...
April 2017: Journal of Huazhong University of Science and Technology. Medical Sciences
https://www.readbyqxmd.com/read/28394397/development-of-perching-behavior-in-3-strains-of-pullets-reared-in-furnished-cages
#10
A M Habinski, L J Caston, T M Casey-Trott, M E Hunniford, T M Widowski
Furnished rearing cages are becoming more widely available to replace conventional systems for pullets. To date, there is little information on how pullets develop perching behavior in furnished cages or how this varies among strains. The objective of this study was to evaluate the use of perches and a platform in a commercial furnished rearing "Combi-Cage" system by 3 pure-bred heritage strains of pullets (Rhode Island Red, Columbian Rock, and White Leghorn). Each cage had 4 elevated locations: one platform and 3 perches of differing heights and positions in the cage...
March 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28388328/efficacy-of-deltagard-%C3%A2-fyfanon-%C3%A2-and-evoluer%C3%A2-31-66-against-culex-quinquefasciatus-in-harris-county-texas
#11
James A Dennett, Pamela M Stark, Chris L Fredregill, Mustapha Debboun
The comparative efficacy of ultra-low volume applications of deltamethrin, permethrin, and malathion against specific adult populations of Culex quinquefasciatus , a primary regional vector of Saint Louis encephalitis and West Nile virus, using field cage tests is described. At labeled application rates, the estimated mass median diameters for Fyfanon(®), Evoluer™, and DeltaGard(®) were 18.32, 19.24, and 17.44 μm, respectively. No significant differences (P ≥ 0.01) in mean percent control were observed across all 3 wild populations and a susceptible Sebring strain tested with Fyfanon, DeltaGard, and Evoluer, providing 98...
March 2017: Journal of the American Mosquito Control Association
https://www.readbyqxmd.com/read/28379554/effect-of-refined-functional-carbohydrates-from-enzymatically-hydrolyzed-yeast-on-the-presence-of-salmonella-spp-in-the-ceca-of-broiler-breeder-females
#12
G K Walker, S Jalukar, J Brake
Broiler breeders hatched from Salmo-nella negative grandparents received either zero or 50 g/MT of refined functional carbohydrates (RFC) in their diets from d of placement to end of lay. There were no other treatments used. Pullets and cockerels were reared separately in an enclosed litter-floor house to 21 wk of age when 28 randomly selected pullets from each diet were transferred to individual cages for an additional 14 d before they were killed, and their ceca were excised aseptically and tested for Salmonella spp...
March 31, 2017: Poultry Science
https://www.readbyqxmd.com/read/28376796/ahcoda-db-a-data-repository-with-web-based-mining-tools-for-the-analysis-of-automated-high-content-mouse-phenomics-data
#13
Bastijn Koopmans, August B Smit, Matthijs Verhage, Maarten Loos
BACKGROUND: Systematic, standardized and in-depth phenotyping and data analyses of rodent behaviour empowers gene-function studies, drug testing and therapy design. However, no data repositories are currently available for standardized quality control, data analysis and mining at the resolution of individual mice. DESCRIPTION: Here, we present AHCODA-DB, a public data repository with standardized quality control and exclusion criteria aimed to enhance robustness of data, enabled with web-based mining tools for the analysis of individually and group-wise collected mouse phenotypic data...
April 4, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28374128/cage-deviation-in-the-subaxial-cervical-spine-in-relation-to-implant-position-in-the-sagittal-plane
#14
Klaus Christian Mende, Sven Oliver Eicker, Friedrich Weber
Subsidence of interbody cages is a frequently observed and relevant complication in anterior cervical discectomy and fusion (ACDF). Only a handful of studies concentrated on the modality of subsidence and its clinical impact. We performed a retrospective analysis of ACDF patients from 2004 to 2010. Numeric analog scale (NAS) score pre-op and post-op, Oswestry Disability Index (ODI) on x-rays, endplate (EP) and cage dimensions, implant position, lordotic/kyphotic subsidence patterns (>5°), and cervical alignment were recorded...
April 4, 2017: Neurosurgical Review
https://www.readbyqxmd.com/read/28372949/sleep-disordered-breathing-in-neuromuscular-disease-diagnostic-and-therapeutic-challenges
#15
REVIEW
Loutfi S Aboussouan, Eduardo Mireles-Cabodevila
Normal sleep related rapid eye movement sleep atonia, reduced lung volumes, reduced chemosensitivity, and impaired airway dilator activity, become significant vulnerabilities in the setting of neuromuscular disease. In that context, the compounding effects of respiratory muscle weakness and disease-specific features that promote upper airway collapse or cause dilated cardiomyopathy, contribute to various sleep-disordered breathing events. The reduction in lung volumes with neuromuscular disease is further compromised by sleep and the supine position, exaggerating the tendency for upper airway collapse and desaturation with sleep-disordered breathing events...
March 31, 2017: Chest
https://www.readbyqxmd.com/read/28372375/adaptive-microfluidic-gradient-generator-for-quantitative-chemotaxis-experiments
#16
Alexander Anielski, Eva K B Pfannes, Carsten Beta
Chemotactic motion in a chemical gradient is an essential cellular function that controls many processes in the living world. For a better understanding and more detailed modelling of the underlying mechanisms of chemotaxis, quantitative investigations in controlled environments are needed. We developed a setup that allows us to separately address the dependencies of the chemotactic motion on the average background concentration and on the gradient steepness of the chemoattractant. In particular, both the background concentration and the gradient steepness can be kept constant at the position of the cell while it moves along in the gradient direction...
March 2017: Review of Scientific Instruments
https://www.readbyqxmd.com/read/28350349/identification-of-conditions-for-successful-aphid-control-by-ladybirds-in-greenhouses
#17
REVIEW
Eric W Riddick
As part of my research on the mass production and augmentative release of ladybirds, I reviewed the primary research literature to test the prediction that ladybirds are effective aphid predators in greenhouses. Aphid population reduction exceeded 50% in most studies and ladybird release rates usually did not correlate with aphid reduction. The ratio of aphid reduction/release rate was slightly less for larvae than adults in some studies, suggesting that larvae were less effective (than adults) in suppressing aphids...
March 28, 2017: Insects
https://www.readbyqxmd.com/read/28345997/evaluation-of-a-new-method-to-determine-the-tibial-tuberosity-advancement-distance-required-to-reduce-the-patellar-tendon-tibial-plateau-angle-to-90%C3%A2-with-the-modified-maquet-technique-in-dogs
#18
Paul Pillard, Veronique Livet, Quentin Cabon, Camille Bismuth, Juliette Sonet, Denise Remy, Didier Fau, Claude Carozzo, Eric Viguier, Thibaut Cachon
OBJECTIVE To assess use of a new radiographic method to determine the distance by which the tibial tuberosity should be advanced to reduce the patellar tendon-tibial plateau angle (PTA) to 90° by means of the modified Maquet technique (MMT) in dogs. SAMPLE 24 pelvic limbs from 12 adult medium-sized to large-breed canine cadavers. PROCEDURES Radiographs of stifle joints at 135° extension in true lateral position were used to determine tibial tuberosity advancement distances for use in the MMT. A method was devised to incorporate the planned osteotomy axis; distal patellar translations of 0, 3, 5, or 10 mm; and advancement cage implant application level along the osteotomy site in advancement planning measurements...
April 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28339968/optimization-of-dietary-zinc-for-egg-production-and-antioxidant-capacity-in-chinese-egg-laying-ducks-fed-a-diet-based-on-corn-wheat-bran-and-soybean-meal
#19
W Chen, S Wang, H X Zhang, D Ruan, W G Xia, Y Y Cui, C T Zheng, Y C Lin
The aim of this study was to evaluate the effect of zinc supplementation on productive performance and antioxidant status in laying ducks. Five-hundred-four laying ducks were divided into 7 treatments, each containing 6 replicates of 12 ducks. The ducks were caged individually and fed a corn-soybean meal and wheat bran basal diet (37 mg Zn/kg) or the basal diet supplemented with 15, 30, 45, 60, 75, or 90 mg Zn/kg (as zinc sulfate). During the early laying period of 10 d (daily egg production <80%), egg production, daily egg mass, and FCR increased quadratically with increasing dietary Zn levels (P < 0...
March 2, 2017: Poultry Science
https://www.readbyqxmd.com/read/28329150/scoliosis-after-pectus-excavatum-correction-does-it-improve-or-worsen-%C3%A2
#20
Jae Ho Chung, Hyung Joo Park, Kwang Taik Kim
OBJECTIVES: Scoliosis is associated with pectus excavatum. However, the change in the degree of scoliosis after pectus excavatum correction has not been clarified. This study examined how the correction of pectus excavatum influences the status of pre-existing scoliosis. METHODS: A total of 779 pectus excavatum patients operated between 2007 and 2011 using the pectus bar were retrospectively analysed. Preoperative severity and postoperative change of scoliosis in accordance with the severity of pectus excavatum were evaluated...
March 7, 2017: European Journal of Cardio-thoracic Surgery
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