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https://www.readbyqxmd.com/read/28808307/lc3a-silencing-hinders-aggresome-vimentin-cage-clearance-in-primary-choroid-plexus-carcinoma
#1
Marwa Nassar, Heba Samaha, Myret Ghabriel, Maha Yehia, Hala Taha, Sherin Salem, Khaled Shaaban, Mariam Omar, Nabil Ahmed, Shahenda El-Naggar
Aggresomes are transient microtubule-dependent inclusion bodies that sequester misfolded proteins and are ultimately removed by autophagy. Here we report the generation of a choroid plexus carcinoma cell line; Children's Cancer Hospital Egypt (CCHE)-45, which is characterized by the constitutive formation of aggresomes. When examining the autophagy pathway as the main route for aggresomes clearance, CCHE-45 cells displayed increased autophagy flux mediated by MAP1LC3B. MAP1LC3A-Variant1 gene expression was silenced by promoter methylation...
August 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28807857/prevalence-and-genetic-characterization-of-toxoplasma-gondii-in-donkeys-in-northeastern-china
#2
Xiao-Xuan Zhang, Wei Shi, Nian-Zhang Zhang, Kun Shi, Jian-Ming Li, Peng Xu, Quan Zhao, Rui Du
Toxolasma gondii is one of the most important obligate intracellular protozoan parasites. Recently, toxoplasmosis is of increasing concerns because T. gondii not only has a worldwide distribution but also can infect virtually all warm-blooded hosts including donkeys. However, limited information is available concerning the genetic characterization of T. gondii in donkeys in northeastern China. In this study, a total of 302 brain tissue samples collected from donkeys from Jilin (n=108) and Liaoning (n=194) provinces, northeastern China, were examined for T...
August 12, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28795702/cascade-photocaging-of-diazeniumdiolate-a-novel-strategy-for-one-and-two-photon-triggered-uncaging-with-real-time-reporting
#3
Krishna Kalyani Behara, Y Rajesh, Yarra Venkatesh, Bhaskar Rao Pinninti, Mahitosh Mandal, N D Pradeep Singh
We report a new strategy, viz. cascade photocaging, for protecting diethylamine diazeniumdiolate (O(2)-position), a light sensitive molecule. Upon photolysis, the cascade photocage at first releases the light activatable linker (latent fluorophore) O(2)-caged diazeniumdiolate, which undergoes spontaneous 1,8-elimination, triggering the release of the diazeniumdiolate anion and the fluorophore.
August 10, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28780576/mechanical-and-surgical-bioprosthetic-valve-thrombosis
#4
REVIEW
Wei Yao Lim, Guy Lloyd, Sanjeev Bhattacharyya
Valve thrombosis can occur in mechanical prosthetic valves and is increasingly recognised in transcatheter and surgically implanted bioprosthetic valves. The risk of thrombosis of mechanical valves is higher in the mitral position compared with aortic position and in older generation valves (ball and cage valves). There is a wide spectrum of presentation from the asymptomatic patient to those with embolic complications or cardiogenic shock. A combination of transthoracic and transesophageal echocardiography is required to assess the haemodynamic effect of thrombosis (valve gradients and area), leaflet motion and thrombus size...
August 5, 2017: Heart: Official Journal of the British Cardiac Society
https://www.readbyqxmd.com/read/28771048/effects-of-nanosized-zinc-oxide-and-%C3%AE-polyglutamic-acid-on-eggshell-quality-and-serum-parameters-of-aged-laying-hens
#5
Syuan-Yian Mao, Tu-Fa Lien
In order to increase zinc (Zn) absorption and improve eggshell quality, diets for aged laying hens were supplemented with different Zn sources and the effects on egg production, eggshell quality and serum parameters were recorded. Seventy-five 64-week old brown layers were individually caged and randomly allotted to five treatment groups: an un-supplemented Control and four groups, where the following Zn sources were added to the diets, ZnO (group ZnO), Zn-methionine (group Zn-Met), nanosized ZnO (group nanoZnO) and γ-PGA-nano ZnO (group γ-PGA-nanoZnO)...
October 2017: Archives of Animal Nutrition
https://www.readbyqxmd.com/read/28765807/the-influence-of-lordotic-cages-on-creating-sagittal-balance-in-the-cmis-treatment-of-adult-spinal-deformity
#6
Neel Anand, Ryan B Cohen, Jason Cohen, Babak Kahndehroo, Sheila Kahwaty, Eli Baron
BACKGROUND: CMIS techniques are heavily dependent on placement of lateral interbody cages. Cages with an increased lordotic angle are being advocated to improve segmental lordosis and SVA. We assessed the segmental lordosis achieved with the individual cages. We further studied three variables and the effect each had on segmental lordosis: the lordosis angle of the cage, the position of the cage in the intervertebral space, and the level that it has been placed. METHODS: This is a retrospective study of 66 consecutive patients who underwent lateral interbody fusion using lordotic cages as part of CMIS correction of scoliosis from June 2012 to January 2016...
2017: International Journal of Spine Surgery
https://www.readbyqxmd.com/read/28759978/a-novel-plif-peek-interbody-cage-with-an-impactionless-insertion-technology-a-case-series-with-a-mid-term-follow-up-of-three-years
#7
Charlotte Struwe, Pia C Hermann, Rahel Bornemann, Milena Plöger, Philip P Roessler, Andreas C Strauss, Yorck Rommelspacher, Ernst M W Koch, Robert Pflugmacher
BACKGROUND: Spinal fusion surgery has become one of the most common spinal procedures during the recent years. Searching for an optimum structural stability of the vertebral interspace, surgical implants which can be inserted via a posterior lumbar interbody fusion (PLIF) approach have been enhanced recently. OBJECTIVE: Evaluation of safety and efficacy of a novel PLIF polyetheretherketone (PEEK) interbody cage (TWIST) with an impactionless insertion technique. METHODS: Surgical outcome in 15 patients treated with the new system were observed preoperatively, one and three years after surgery using the Oswestry Disability Index (ODI), a Visual Analogue Scale (VAS) for pain and radiological outcome analysis of fusion success...
July 21, 2017: Technology and Health Care: Official Journal of the European Society for Engineering and Medicine
https://www.readbyqxmd.com/read/28758213/observations-of-marine-wildlife-tourism-effects-on-a-non-focal-species
#8
J R Rizzari, J M Semmens, A Fox, C Huveneers
A radio-acoustic positioning system was used to assess the effects of shark cage-diving operators (SCDO) on the fine-scale movements of a non-focal species, the smooth stingray Bathytoshia brevicaudata. The results revealed that the time spent in the array was individually variable, but generally increased when SCDO were present and that the presence of SCDO may have the capacity to elicit changes in the space use of B. brevicaudata. These results indicate that the effects of marine wildlife tourism may extend beyond the focal species of interest...
July 30, 2017: Journal of Fish Biology
https://www.readbyqxmd.com/read/28752864/co-diffusion-as-a-re-orientation-mechanism-in-the-nay-zeolite
#9
A A Rybakov, A V Larin, D P Vercauteren
Our work is devoted to DFT calculations of the relative rotational and diffusional barriers for CO motions in zeolite NaY. The diffusion jump of CO adsorbed in NaY from NaII to Na'II has been confirmed as the favored way for CO re-coordination via either the C or the O atom to the Na cations instead of the CO rotation, hence explaining the mechanism which is responsible for the CO exchange between different positions and the changes in the intensities of the vibrational IR spectra. The fine structure of the vibrational C-O bands is explained by the different CO locations of adsorbed mono- and dicarbonyl species...
August 9, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28736210/effects-of-positive-expiratory-pressure-on-chest-wall-volumes-in-subjects-with-stroke-compared-to-healthy-controls-a-case-control-study
#10
Elis E A Cabral, Vanessa R Resqueti, Illia N D F Lima, Lucien P Gualdi, Andrea Aliverti, Guilherme A F Fregonezi
BACKGROUND: Alterations in respiratory system kinematics in stroke lead to restrictive pattern associated with decreased lung volumes. Chest physical therapy, such as positive expiratory pressure, may be useful in the treatment of these patients; however, the optimum intensity to promote volume and motion changes of the chest wall remains unclear. OBJECTIVE: To assess the effect of different intensities of positive expiratory pressure on chest wall kinematics in subjects with stroke compared to healthy controls...
July 8, 2017: Brazilian Journal of Physical Therapy
https://www.readbyqxmd.com/read/28730435/supported-fluid-lipid-bilayer-as-a-scaffold-to-direct-assembly-of-rna-nanostructures
#11
Aleksandra P Dabkowska, Agnes Michanek, Luc Jaeger, Arkadiusz Chworos, Tommy Nylander, Emma Sparr
RNA architectonics offers the possibility to design and assemble RNA into specific shapes, such as nanoscale 3D solids or nanogrids. Combining the minute size of these programmable shapes with precise positioning on a surface further enhances their potential as building blocks in nanotechnology and nanomedicine. Here we describe a bottom-up approach to direct the arrangement of nucleic acid nanostructures by using a supported fluid lipid bilayer as a surface scaffold. The strong attractive electrostatic interactions between RNA polyanions and cationic lipids promote RNA adsorption and self-assembly...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28724490/murine-astrovirus-infection-and-transmission-in-neonatal-cd1-mice
#12
Susan R Compton, Carmen J Booth, James D Macy
Murine astrovirus (MuAstV) is a recently identified, widespread infection among laboratory mice. Our goal was to determine the duration of MuAstV infection, susceptibility of pups, and efficacy of soiled-bedding sentinels and environmental monitoring. Eight CD1 dams and their litters of 3-d-old pups and 8 CD1 dams and their litters of 13-d-old mice were inoculated orally with MuAstV. Neither dams nor offspring demonstrated any clinical signs, and MuAstV had little to no effect on weight gain in pups. MuAstV RNA was detected in feces from 15 of the 16 dams through postnatal day (PND) 21, and 9 dams were still shedding MuAstV at PND 42...
July 1, 2017: Journal of the American Association for Laboratory Animal Science: JAALAS
https://www.readbyqxmd.com/read/28722076/the-anomalous-halogen-bonding-interactions-between-chlorine-and-bromine-with-water-in-clathrate-hydrates
#13
Hana Dureckova, Tom K Woo, Konstantin A Udachin, John A Ripmeester, Saman Alavi
Clathrate hydrate phases of Cl2 and Br2 guest molecules have been known for about 200 years. The crystal structure of these phases was recently re-determined with high accuracy by single crystal X-ray diffraction. In these structures, the water oxygen-halogen atom distances are determined to be shorter than the sum of the van der Waals radii, which indicates the action of some type of non-covalent interaction between the dihalogens and water molecules. Given that in the hydrate phases both lone pairs of each water oxygen atom are engaged in hydrogen bonding with other water molecules of the lattice, the nature of the oxygen-halogen interactions may not be the standard halogen bonds characterized recently in the solid state materials and enzyme-substrate compounds...
July 19, 2017: Faraday Discussions
https://www.readbyqxmd.com/read/28712092/favorable-impact-on-stress-related-behaviors-by-modulating-plasma-butyrylcholinesterase
#14
REVIEW
Stephen Brimijoin, Susannah Tye
In the last decade, it has become clear that the neuropeptide "ghrelin" and its principal receptor have a large impact on anxiety and stress. Our recent studies have uncovered a link between plasma butyrylcholinesterase (BChE) and ghrelin. BChE actually turns out to be the key regulator of this peptide. This article reviews our recent work on manipulating ghrelin levels in mouse blood and brain by long term elevation of BChE, leading to sustained decrease of ghrelin. That effect in turn was found to reduce stress-induced aggression in group caged mice...
July 15, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28709707/endovascular-treatment-of-acute-ischemic-stroke-with-eric-device
#15
Laurent Pierot, Jean-Yves Gauvrit, Vincent Costalat, Michel Piotin, Charbel Mounayer, Denis Herbreteau, Sophie Gallas, René Anxionnat, Hubert Desal
BACKGROUND AND PURPOSE: After 6 positive randomized trials, mechanical thrombectomy with stent-retriever is now recommended as a first-line treatment for acute ischemic stroke (AIS). The ERIC device is a device with several interlinked cage-like spheres fixed on a pusher wire. Neurothrombectomy France (NTF) is a registry conducted in France to analyze the results of mechanical thrombectomy. In order to analyze its performances, the subgroup of patients treated with the ERIC device was analyzed...
July 11, 2017: Journal of Neuroradiology. Journal de Neuroradiologie
https://www.readbyqxmd.com/read/28705503/building-a-bio-based-hydrogel-via-electrostatic-and-host-guest-interactions-for-realizing-dual-controlled-release-mechanism
#16
Shuai Han, Ting Wang, Li Yang, Bin Li
Bio-based hydrogel containing cyclodextrins (CDs) is of a promising polymer material that could display many advantages including wide availability, sustainability, biocompatibility and biodegradability, especially the inherent encapsulation ability with hydrophobic substance. To obtain these, the electrostatic and host-guest interactions were introduced and a hydrogel with three-dimensional double network structures was built. For preparing a spherical biopolymer cage, hydroxyethyl cellulose (HEC) and modified chitosan (HACC) were cross-linked by a one-pot reaction...
July 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28704856/effects-of-highly-pure-enriched-cellulose-diet-on-digestibility-lipid-metabolism-and-redox-status-in-obese-offspring-of-streptozotocin-induced-diabetic-rats
#17
Bensalah Meryem, Bouanane Samira, Benyagoub Ouahiba, Merzouk Hafida, Baba Ahmed Fatima Zohra
The long-term nutritional efficacy of a highly-pure-enriched-cellulose (HPEC) diet and its effects on lipid metabolism and oxidant/antioxidant status were investigated in obese offspring of streptozotocin-induced diabetic rats. Diabetes in pregnant rats was induced by intraperitoneal injection of streptozotocin on day 5 of gestation. Control pregnant rats were injected with citrate buffer. At weaning offspring of diabetic and control rats were fed HPEC or control diet, they were housed individually in metabolic cage for 2 months to investigate the nutritional efficacy of the diets...
July 13, 2017: Experimental and Clinical Endocrinology & Diabetes
https://www.readbyqxmd.com/read/28704473/enriched-environment-and-stress-exposure-influence-splenic-b-lymphocyte-composition
#18
Blake T Gurfein, Burcu Hasdemir, Jeffrey M Milush, Chadi Touma, Rupert Palme, Douglas F Nixon, Nicholas Darcel, Frederick M Hecht, Aditi Bhargava
Prolonged chronic stress has deleterious effects on immune function and is associated with numerous negative health outcomes. The spleen harbors one-fourth of the body's lymphocytes and mediates both innate and adaptive immune responses. However, the subset of splenic lymphocytes that respond, either adaptively or maladaptively, to various stressors remains largely unknown. Here we investigated the effects of unpredictable chronic mild stress (CMS) exposure on spleen composition in male mice housed in two different caging conditions: standard caging (Cntl) and enriched environment (EE)...
2017: PloS One
https://www.readbyqxmd.com/read/28704331/mis-single-position-lateral-and-oblique-lateral-lumbar-interbody-fusion-and-bilateral-pedicle-screw-fixation-feasibility-and-perioperative-results
#19
Daniel J Blizzard, J Alex Thomas
STUDY DESIGN: Retrospective review of prospectively collected data of the first 72 consecutive patients treated with single-position one- or two-level lateral (LLIF) or oblique lateral interbody fusion (OLLIF) with bilateral percutaneous pedicle screw and rod fixation by a single spine surgeon. OBJECTIVE: To evaluate the clinical feasibility, accuracy, and efficiency of a single-position technique for LLIF and OLLIF with bilateral pedicle screw and rod fixation...
July 12, 2017: Spine
https://www.readbyqxmd.com/read/28700052/influence-of-environmental-enrichment-on-the-behavior-and-physiology-of-mice-infected-by-trypanosoma-cruzi
#20
Déborah Maria Moreira da Silva, Laila Pinheiro, Cristiano Schetini Azevedo, Guilherme de Paula Costa, André Talvani
INTRODUCTION: Enriched environments normally increase behavioral repertoires and diminish the expression of abnormal behaviors and stress-related physiological problems in animals. Although it has been shown that experimental animals infected with microorganisms can modify their behaviors and physiology, few studies have evaluated how environmental enrichment affects these parameters. This study aimed to evaluate the effects of environmental enrichment on the behavior and physiology of confined mice infected with Trypanosoma cruzi...
May 2017: Revista da Sociedade Brasileira de Medicina Tropical
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