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D Buiuc, C Bercovici, C Iosub, C Straton
The antibiogram of 658 S. typhi strains isolated in Moldova during the last 20 years points out that this bacterium is preserving in the studied area its natural sensitivity towards the antibiotics used in the treatment of typhoid fever. A very small number of strains resistant to these antibiotics (below 0.5%) have appeared as an accident without clinico-epidemiological consequences. The MIC unimodal distribution of cloramphenicol and other antibiotics towards S. typhi is similar to the MIC distribution towards the serotypes with very limited circulation (S...
January 1982: Revista Medico-chirurgicală̆ a Societă̆ţ̜ii de Medici ş̧i Naturaliş̧ti Din Iaş̧i
Torsten Meyer, Thomas Schirrmann, André Frenzel, Sebastian Miethe, Janin Stratmann-Selke, Gerald F Gerlach, Katrin Strutzberg-Minder, Stefan Dübel, Michael Hust
BACKGROUND: Solely in Europoe, Salmonella Typhimurium causes more than 100,000 infections per year. Improved detection of livestock colonised with S. Typhimurium is necessary to prevent foodborne diseases. Currently, commercially available ELISA assays are based on a mixture of O-antigens (LPS) or total cell lysate of Salmonella and are hampered by cross-reaction. The identification of novel immunogenic proteins would be useful to develop ELISA based diagnostic assays with a higher specificity...
2012: BMC Biotechnology
Michael R McLaughlin, John P Brooks, Ardeshir Adeli, John J Read
Swine (Sus scofa domestica) lagoon effluent is a valuable resource. In the U.S. Mid-South it is applied from April to September to fertilize grass hay in spray-irrigated fields. Lagoon levels of nutrients and bacteria, and soil levels of nutrients have been documented, but little was known of effluent bacterial levels in soil. The present study examined levels of selected effluent bacteria and nutrients in soils inside and outside spray fields after >15 yr of effluent irrigation. Samples were collected February to March 2009 from contiguous soils spanning adjacent irrigated and nonirrigated areas...
September 2010: Journal of Environmental Quality
Yiying Qin, Hui Wu, Xinglong Xiao, Xiaoquan Yang, Jingwei Zhang, Yigang Yu, Huifang Li
UNLABELLED: We designed a pair of specific primers and a TaqMan fluorescent probe targeting the toxR gene of Vibrio parahaemolyticus (VP). After optimizing the conditions, the specialty, sensitivity and reproducibility of the detection method were evaluated. RESULTS: (1) the developed real-time PCR assay protocol detected only VP and was not affected by other normal food pathogens such as Staphylococcus aureus, Salmonela, Listeria monocytogenes. (2) the limit of detection was 25 copies of toxR gene in the detected samples, and the sensitivity of pure cultures and simulated food samples was 21 cfu/mL and 210 cfu/g...
October 2008: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
Lillian C Becker
3-Methylamino-4-Nitrophenoxyethanol is a semipermanent (direct) hair colorant used in 21 hair dyes and colors at use concentrations up to 0.15%. When applied to human skin in vitro, 0.42% of the applied 3-Methylamino-4-Nitrophenoxyethanol was recovered in the receptor fluid. In an acute toxicity study using rats, 3-Methylamino-4-Nitrophenoxyethanol at 1000 mg/kg resulted in hypoactivity, piloerection, dyspnea, and lateral recumbency in animals that later died. The surviving rats exhibited none of these signs...
2008: International Journal of Toxicology
Yoshiyuki Yamazaki, Ken-Ichi Fujita, Kazuo Nakayama, Akihiro Suzuki, Katsunori Nakamura, Hiroshi Yamazaki, Tetsuya Kamataki
We newly developed 10 Salmonela typhimurium TA1538 strains each co-expressing a form of human cytochrome P450s (P450 or CYP) together with NADPH-cytochrome P450 reductase (CPR) for highly sensitive detection of mutagenic activation of mycotoxins, polycyclic aromatic hydrocarbons, heterocyclic amines, and aromatic amines at low substrate concentrations. Each form of P450 (CYP1A1, CYP1A2, CYP2A6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A4 or CYP3A5) expressed in the TA1538 cells efficiently catalyzed the oxidation of a representative substrate...
August 8, 2004: Mutation Research
A Jafari, S Bouzari, A A Farhoudi-Moghaddam, M Parsi, F Shokouhi
A total of 132 isolates of the Havana serovar of Salmonela enterica were studied for their adhesion to and invasion of Hela cells in the presence of D-mannose. A total of 124 (93.9%) isolates were adherent, and 51% of these were invasive. Of these 124 strains 82 (66%) adhered to HeLa cells with a pattern typical for enteroaggregative (EAgg) Escherichia coli whereas the remaining 34% adhered in a non-aggregative (non-EAgg) pattern. Of the 82 strains showing the EAgg pattern 60% were invasive whereas only 33% of the 49 non-EAgg isolates invaded HeLa cells (P < 0...
January 1994: Microbial Pathogenesis
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