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Streptococcus equi

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https://www.readbyqxmd.com/read/29778182/evidence-for-involvement-of-the-fas-bcax-regulon-in-capsule-synthesis-by-streptococcus-equi
#1
Sridhar Velineni, John F Timoney
The constitutively expressed hyaluronic acid capsule is an important virulence factor of Streptococcus equi, the cause of equine strangles. Study of the genomic sequence of CF22caps-, a non-encapsulated mutant of S. equi CF22 generated by gamma (Co60 ) irradiation revealed a non-sense mutation in fasC (SEQ_0302), a sensor kinase gene in FasBCAX an operon with an important regulatory role in expression of streptococcal secreted virulence and matrix binding proteins. The mutation was associated with a significant (p < ...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29761571/frequency-of-shedding-of-respiratory-pathogens-in-horses-recently-imported-to-the-united-states
#2
Fauna Leah Smith, Johanna L Watson, Sharon J Spier, Isabelle Kilcoyne, Samantha Mapes, Claudia Sonder, Nicola Pusterla
BACKGROUND: Imported horses that have undergone recent long distance transport might represent a serious risk for spreading infectious respiratory pathogens into populations of horses. OBJECTIVE: To investigate the frequency of shedding of respiratory pathogens in recently imported horses. ANIMALS: All imported horses with signed owner consent (n = 167) entering a USDA quarantine for contagious equine metritis from October 2014 to June 2016 were enrolled in the study...
May 15, 2018: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29758536/characterization-of-sesec_01411-as-a-surface-protective-antigen-of-streptococcus-equi-ssp-zooepidemicus
#3
Honglin Xie, Zigong Wei, Chunquan Ma, Shun Li, Xiaohong Liu, Qiang Fu
Streptococcus equi ssp. zooepidemicus (Streptococcus zooepidemicus, SEZ) is a commensal bacterium related to opportunistic infections of many species, including humans, dogs, cats, and pigs. SeseC_01411 has been proven to be immunogenic. However, its protective efficacy remained to be evaluated. In the present study, the purified recombinant SeseC_01411 could elicit a strong humoral antibody response and protect against lethal challenge with virulent SEZ in mice. Our finding confirmed that SeseC_01411 distributes on the surface of SEZ...
May 8, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29717639/streptococcus-equi-subspecies-zooepidemicus-septicemia-in-alpacas-three-cases-and-review-of-the-literature
#4
Juan M Corpa, Francisco Carvallo, Mark L Anderson, Akinyi C Nyaoke, Janet D Moore, Francisco A Uzal
Streptococcus equi subspecies zooepidemicus septicemia of alpacas and llamas, also called alpaca fever, is characterized clinically by fever, depression, recumbency, and death, and pathologically by polyserositis. Although a few natural and experimental cases of the disease have been reported, very little information about the pathology of spontaneous cases has been published. We present a detailed gross and microscopic description of 3 spontaneous cases of alpaca fever and review the literature on this condition...
April 1, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29691005/evaluation-of-magnetic-nanoparticles-influence-on-hyaluronic-acid-production-from-streptococcus-equi
#5
Yasser A Attia, Mohamed I Kobeasy, Mohamed Samer
This work aims at developing a novel hyaluronic acid (HA) production method using magnetic nanoparticles (NPs). In a separate process, HA was produced with the addition of the amino acids (AA) as bio-additives. Regarding the NPs additives, the results showed that the highest dry weight of the produced HA was 0.264 g/l with the addition of 20 mg/l of Fe3 O4 NPs. Concerning the AA additives, the results showed that the highest dry weight of the produced HA was 0.079 g/l with the addition of 0.26 g/l of glutamic acid (GA) compared to the control produced 0...
July 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29626984/the-proteome-of-fetal-fluids-in-mares-with-experimentally-induced-placentitis
#6
S C Loux, B A Ball
INTRODUCTION: Placental inflammation (placentitis) is one of the leading causes of late-term abortion in mares. Although prognosis is good assuming early diagnosis and treatment, diagnostics are limited. METHODS: To better characterize the disease and identify potential biomarkers, we analyzed the proteome of fetal fluids (amniotic and allantoic) in both control mares (n = 5) and mares with experimentally-induced placentitis (n = 5) using LTQ-Orbitrap mass-spectrometry...
April 2018: Placenta
https://www.readbyqxmd.com/read/29583047/phagocytosis-bacterial-killing-and-cytokine-activation-of-circulating-blood-neutrophils-in-horses-with-severe-equine-asthma-and-control-horses
#7
Johanne M Vanderstock, Marie-Pier Lecours, Annouck Lavoie-Lamoureux, Marcelo Gottschalk, Mariela Segura, Jean-Pierre Lavoie, Daniel Jean
OBJECTIVE To evaluate in vitro phagocytosis and bactericidal activity of circulating blood neutrophils in horses with severe equine asthma and control horses and to determine whether circulating blood neutrophils in horses with severe equine asthma have an increase in expression of the proinflammatory cytokine tumor necrosis factor (TNF)-α and the chemokine interleukin (IL)-8 and a decrease in expression of the anti-inflammatory cytokine IL-10 in response to bacteria. ANIMALS 6 horses with severe equine asthma and 6 control horses...
April 2018: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/29574904/respiratory-disease-and-sero-epidemiology-of-respiratory-pathogens-in-the-working-horses-of-ethiopia
#8
G Laing, R Christley, A Stringer, N Aklilu, T Ashine, R Newton, A Radford, G Pinchbeck
BACKGROUND: Pathogens are frequently implicated in equine respiratory disease. In Ethiopia, respiratory disease is a frequent cause for presentation at veterinary clinics and a priority concern for users of working horses. However, there is little existing literature on possible aetiologies. OBJECTIVES: Determine prevalence of respiratory signs and exposure to major respiratory pathogens through a serological survey. STUDY DESIGN: Cross-sectional...
March 25, 2018: Equine Veterinary Journal
https://www.readbyqxmd.com/read/29545493/-streptococcus-equi-breaking-its-strangles-hold
#9
Andrew Waller
Andrew Waller , head of bacteriology at the AHT, describes Streptococcus equi, the causative agent of strangles in horses, and discusses progress with the latest research aimed at improving vaccines against this global disease.
March 17, 2018: Veterinary Record
https://www.readbyqxmd.com/read/29424487/streptococcus-equi-infections-in-horses-guidelines-for-treatment-control-and-prevention-of-strangles-revised-consensus-statement
#10
A G Boyle, J F Timoney, J R Newton, M T Hines, A S Waller, B R Buchanan
This consensus statement update reflects our current published knowledge and opinion about clinical signs, pathogenesis, epidemiology, treatment, complications, and control of strangles. This updated statement emphasizes varying presentations in the context of existing underlying immunity and carrier states of strangles in the transmission of disease. The statement redefines the "gold standard" for detection of possible infection and reviews the new technologies available in polymerase chain reaction diagnosis and serology and their use in outbreak control and prevention...
March 2018: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29398274/strangvac-a-recombinant-fusion-protein-vaccine-that-protects-against-strangles-caused-by-streptococcus-equi
#11
Carl Robinson, Lars Frykberg, Margareta Flock, Bengt Guss, Andrew S Waller, Jan-Ingmar Flock
The host-restricted pathogen Streptococcus equi causes strangles in the horse, which is characterised by abscessation of the lymph nodes of the head and neck. The disease is endemic throughout the world causing considerable welfare and economic cost to the horse industry. Here we report the results of three studies where ponies were vaccinated with combinations of recombinant fusion proteins to optimise vaccine production and the level of protection conferred. Optimal protection was conferred by a prototype multicomponent subunit vaccine, Strangvac 4, which contained eight proteins CNE, SclC, SclF, SclI, EAG (fused as CCE), SEQ_402, SEQ_0256 (fused as Eq85) and IdeE...
March 7, 2018: Vaccine
https://www.readbyqxmd.com/read/29377359/decreased-clinical-severity-of-strangles-in-weanlings-associated-with-restricted-seroconversion-to-optimized-streptococcus-equi-ssp-equi-assays
#12
L Tscheschlok, M Venner, K Steward, R Böse, M Riihimäki, J Pringle
BACKGROUND: Streptococcus equi ssp. equi causes characteristic clinical signs that are most severe in young horses, including fever, purulent nasal discharge, and lymph node abscessation in the head region. HYPOTHESIS/OBJECTIVES: Clinical, serologic, and microbiologic factors related to unexpectedly mild disease severity in a natural outbreak of strangles in immunologically naïve weanlings were investigated. ANIMALS: One-hundred and twelve warmblood weanlings...
January 2018: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29341315/use-of-quantitative-real-time-pcr-to-determine-viability-of-streptococcus-equi-subspecies-equi-in-respiratory-secretions-from-horses-with-strangles
#13
N Pusterla, C M Leutenegger, S M Barnum, B A Byrne
BACKGROUND: In recent years, molecular approaches have been able to characterise the viability of equine upper respiratory tract pathogens using absolute molecular quantitation as well as detection of transcripts for virulence genes. OBJECTIVES: The objective this study was to investigate molecular surrogates for S. equi subspecies equi (S. equi) viability in biological samples from horses with strangles. STUDY DESIGN: Retrospective cross-sectional study...
January 17, 2018: Equine Veterinary Journal
https://www.readbyqxmd.com/read/29306502/identification-and-characterization-of-a-novel-protective-antigen-sec_205-of-streptococcus-equi-ssp-zooepidemicus
#14
Huihuang Liang, Bin Tang, Pengpeng Zhao, Mingyong Deng, Lili Yan, Pan Zhai, Zigong Wei
Streptococcus equi ssp. zooepidemicus (SEZ) is an important pathogen of swine streptococcal diseases and can infect a wide range of animals as well as human beings. The absence of effective vaccine confounds the control of SEZ infection. Sec_205, a novel protein identified in the previous study, was inducibly over-expressed in Escherichia coli in the present study. The purified recombinant protein could elicit a significant humoral antibody response and provide efficient protection against lethal challenge of SEZ C55138 in mouse model...
February 1, 2018: Vaccine
https://www.readbyqxmd.com/read/29198841/-antimicrobial-susceptibility-in-isolates-of-streptococcus-equi-subsp-equi-from-buenos-aires-province-argentina
#15
Carla P Bustos, María J Marfil, Natalia S Lanza, Nora Guida
Streptococcus equi subsp. equi is the etiologic agent of strangles, an infectious disease affecting the upper respiratory tract and head and neck lymph nodes of equines. Routine antimicrobial therapy includes penicillin (PEN) as antibiotic of first choice. Streptococci are usually susceptible to PEN and only a few antimicrobial studies had been performed. The aim of this work was to study the antimicrobial susceptibility profile of S. equi from Buenos Aires, Argentina. Ninety-two isolates were studied by the single disk method to PEN, cefotaxime, erythromycin (ERY), tetracycline, enrofloxacin (ENR), trimethoprim sulfamethoxazole (TMS), ciprofloxacin, clindamycin (CLI), streptomycin (STR) and florfenicol...
November 30, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/29164073/-streptococcus-equi-subsp-zooepidemicus-invades-and-survives-in-epithelial-cells
#16
Bolette Skive, Manfred Rohde, Gabriella Molinari, Thomas Hartig Braunstein, Anders M Bojesen
Streptococcus equi subsp. zooepidemicus ( S. zooepidemicus ) is an opportunistic pathogen of several species including humans. S. zooepidemicus is found on mucus membranes of healthy horses, but can cause acute and chronic endometritis. Recently S. zooepidemicus was found able to reside in the endometrium for prolonged periods of time. Thus, we hypothesized that an intracellular phase may be part of the S. zooepidemicus pathogenesis and investigated if S. zooepidemicus was able to invade and survive inside epithelial cells...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29147933/aerobic-bacteriological-studies-on-the-respiratory-tracts-of-apparently-healthy-and-pneumonic-camels-camelus-dromedaries-in-selected-districts-of-afar-region-ethiopia
#17
Mu'uz Gebru, Genene Tefera, Fufa Dawo, Tesfaye Sisay Tessema
A cross-sectional study was conducted to isolate and identify bacterial species from the respiratory tract of apparently healthy and pneumonic camels in Asayita and Dubti woredas in the Afar Region, Ethiopia. From a total of 74 lung tissue and 74 tracheal swab samples Staphylococcus aureus, 16.3%, Streptococcus equi subsp. equi, 13.0%, and Pasteurella multocida, 10.9%, were dominant isolates from pneumonic lungs; Escherichia coli, 12.7%, Proteus species, 10.9%, and Klebsiella pneumoniae, 9.1%, were the majority in the normal lungs...
March 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29103694/mir-194b-3p-partially-inhibits-streptococcus-equi-subsp-zooepidemicus-adherence-to-pk15-cells
#18
Bin Tang, Huihuang Liang, Pengpeng Zhao, Zigong Wei
MicroRNAs are increasingly reported implicated in the host cell response to bacterial pathogens. In order to investigate whether miR-194b-3p regulates the adherence of Streptococcus equi subsp. Zooepidemicus (SeZ) to porcine kidney cell line PK15, the miR-194b-3p agomir and antagomir were transfected into PK15 cells respectively and the adherence rate of SeZ to each was determined. Adherence rate of SeZ C55138 was significantly decreased when miR-194b-3p agomir was transfected in PK15, while that of miR-194b-3p antagomir evaluated...
October 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/29048299/blood-culture-negative-endocarditis-morocco
#19
Najma Boudebouch, M'hammed Sarih, Abdelfattah Chakib, Salma Fadili, Drissi Boumzebra, Zahira Zouizra, Badie Azamane Mahadji, Hamid Amarouch, Didier Raoult, Pierre-Edouard Fournier
We investigated the microorganisms causing blood culture-negative endocarditis (BCNE) in Morocco. We tested 19 patients with BCNE by serologic methods, molecular methods, or both and identified Bartonella quintana, Staphylococcus aureus, Streptococcus equi, and Streptococcus oralis in 4 patients. These results highlight the role of these zoonotic agents in BCNE in Morocco.
November 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29018124/straight-from-the-horse-s-mouth-genomic-epidemiology-of-an-icelandic-equine-epidemic
#20
Mark R Davies
Despite tight biosecurity measures, an outbreak of respiratory disease rapidly spread across the Icelandic equine population in 2010. Horse transportation was brought to a halt in order to contain the spread of the infectious agent. In a recent article, Björnsdóttir and colleagues (S. Björnsdóttir et al., mBio 8:e00826-17, 2017, https://doi.org/10.1128/mBio.00826-17) employ the power and resolution of "genomic epidemiology," the combination of whole genomic sequencing and epidemiological approaches, to examine the source and spread of the outbreak...
October 10, 2017: MBio
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