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https://www.readbyqxmd.com/read/28813482/cysteine-rich-protein-2-accelerates-actin-filament-cluster-formation
#1
Takanori Kihara, Yasunobu Sugimoto, Satoko Shinohara, Shunpei Takaoka, Jun Miyake
Filamentous actin (F-actin) forms many types of structures and dynamically regulates cell morphology and movement, and plays a mechanosensory role for extracellular stimuli. In this study, we determined that the smooth muscle-related transcription factor, cysteine-rich protein 2 (CRP2), regulates the supramolecular networks of F-actin. The structures of CRP2 and F-actin in solution were analyzed by small-angle X-ray solution scattering (SAXS). The general shape of CRP2 was partially unfolded and relatively ellipsoidal in structure, and the apparent cross sectional radius of gyration (Rc) was about 15...
2017: PloS One
https://www.readbyqxmd.com/read/28811226/characterization-and-expression-analysis-of-six-interleukin-17-receptor-genes-in-grouper-epinephelus-coioides-after-cryptocaryon-irritans-infection
#2
Biao Jiang, Yan-Wei Li, Ya-Zhou Hu, Heng-Li Luo, An-Xing Li
Interleukin-17 receptors (IL17Rs) mediate the activation of several downstream signal pathways to induce inflammatory response and contribute to the pathology of many autoimmune diseases. In this study, six IL17Rs (IL17RA1, RA2, RB, RC, RD and RE) were cloned and characterized from Epinephelus coioides, an orange-spotted grouper. Multiple sequence alignment and structural analysis revealed that all members of IL17Rs were low in sequence identity with each other. But their structures were conservative in grouper, which contain signal peptide, extracellular FNIII domain (IL17RA1/RA2/RB) or IL-17_R_N domain (IL17RC/RD/RE), transmembrane domain and SEFIR domain in their intracellular region...
August 12, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28810684/a-new-approach-combining-analytical-methods-for-workplace-exposure-assessment-of-inhalable-multi-walled-carbon-nanotubes
#3
Peter C Tromp, Eelco Kuijpers, Cindy Bekker, Lode Godderis, Qing Lan, Aleksandra D Jedynska, Roel Vermeulen, Anjoeka Pronk
To date there is no consensus about the most appropriate analytical method for measuring carbon nanotubes (CNTs), hampering the assessment and limiting the comparison of data. The goal of this study is to develop an approach for the assessment of the level and nature of inhalable multi-wall CNTs (MWCNTs) in an actual workplace setting by optimizing and evaluating existing analytical methods. In a company commercially producing MWCNTs, personal breathing zone samples were collected for the inhalable size fraction with IOM samplers; which were analyzed with carbon analysis, inductively coupled plasma mass spectrometry (ICP-MS) and scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM/EDX)...
August 1, 2017: Annals of Work Exposures and Health
https://www.readbyqxmd.com/read/28809138/daejeonia-ginsenosidivorans-gen-nov-sp-nov-a-ginsenoside-transforming-bacterium-isolated-from-lake-water
#4
Muhammad Zubair Siddiqi, Gyu-Min Choi, Mi-Sun Kim, Wan-Taek Im
A Gram-stain-negative, oxidase- and catalase-positive, strictly aerobic, non-gliding bacterial strain, designated as NPT, was isolated from lake water and subjected to a taxonomic study using a polyphasic approach. Colonies of strain NP5T were light-yellow-coloured, circular with regular margins, and opaque. Cells were rods, 0.2-0.5 µm wide and 1.2-3.0 µm long. Strain NP5T possessed β-glycosidase activity, which was responsible for its ability to convert ginsenosides Rb1, Rc and Rd (three main active components of ginseng) to ginsenoside F2...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28807374/design-and-application-of-discrete-wavelet-packet-transform-based-multiresolution-controller-for-liquid-level-system
#5
Rimi Paul, Anindita Sengupta
A new controller based on discrete wavelet packet transform (DWPT) for liquid level system (LLS) has been presented here. This controller generates control signal using node coefficients of the error signal which interprets many implicit phenomena such as process dynamics, measurement noise and effect of external disturbances. Through simulation results on LLS problem, this controller is shown to perform faster than both the discrete wavelet transform based controller and conventional proportional integral controller...
August 11, 2017: ISA Transactions
https://www.readbyqxmd.com/read/28805902/enteric-methane-emissions-from-low-and-high-residual-feed-intake-beef-heifers-measured-using-greenfeed-and-respiration-chamber-techniques
#6
A W Alemu, D Vyas, G Manafiazar, J A Basarab, K A Beauchemin
The objectives of this study were to evaluate the relationship between residual feed intake (RFI; g/d) and enteric methane (CH) production (g/kg DM) and to compare CH and carbon dioxide (CO) emissions measured using respiration chambers (RC) and the GreenFeed emission monitoring (GEM) system (C-Lock Inc., Rapid City, SD). A total of 98 crossbred replacement heifers were group housed in 2 pens and fed barley silage ad libitum and their individual feed intakes were recorded by 16 automated feeding bunks (GrowSafe, Airdrie, AB, Canada) for a period of 72 d to determine their phenotypic RFI...
August 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28805849/topology-of-polymer-chains-under-nanoscale-confinement
#7
Vahid Satarifard, Maziar Heidari, Samaneh Mashaghi, Sander J Tans, Mohammad Reza Ejtehadi, Alireza Mashaghi
Spatial confinement limits the conformational space accessible to biomolecules but the implications for bimolecular topology are not yet known. Folded linear biopolymers can be seen as molecular circuits formed by intramolecular contacts. The pairwise arrangement of intra-chain contacts can be categorized as parallel, series or cross, and has been identified as a topological property. Using molecular dynamics simulations, we determine the contact order distributions and topological circuits of short semi-flexible linear and ring polymer chains with a persistence length of lp under a spherical confinement of radius Rc...
August 14, 2017: Nanoscale
https://www.readbyqxmd.com/read/28805264/large-nested-variant-of-urothelial-carcinoma-a%C3%A2-clinicopathological-study-of-36-cases
#8
Eva Compérat, Jesse K McKenney, Arndt Hartmann, Ondrej Hes, Simone Bertz, Justine Varinot, Fadi Brimo
AIMS: The large nested variant of urothelial carcinoma (LNUC) has been added to the World Health Organization (WHO) 2016 classification. Scant data exist, and little is known about its clinical behaviour. MATERIAL AND RESULTS: Cases fulfilling the morphological criteria of LNUC were collected. Pure and mixed cases (i.e. with other patterns of invasive UC) were studied. Immunohistochemical staining with cytokeratin (CK)7, p63, GATA-3, CK20, p53 and Ki-67 was performed...
June 9, 2017: Histopathology
https://www.readbyqxmd.com/read/28803783/impact-of-mutations-within-the-fe-s-cluster-or-the-lipoic-acid-biosynthesis-pathways-on-mitochondrial-protein-expression-profiles-in-fibroblasts-from-patients
#9
E Lebigot, P Gaignard, I Dorboz, A Slama, M Rio, P de Lonlay, B Héron, F Sabourdy, O Boespflug-Tanguy, A Cardoso, F Habarou, C Ottolenghi, P Thérond, C Bouton, M P Golinelli-Cohen, A Boutron
Lipoic acid (LA) is the cofactor of the E2 subunit of mitochondrial ketoacid dehydrogenases and plays a major role in oxidative decarboxylation. De novo LA biosynthesis is dependent on LIAS activity together with LIPT1 and LIPT2. LIAS is an iron‑sulfur (Fe-S) cluster-containing mitochondrial protein, like mitochondrial aconitase (mt-aco) and some subunits of respiratory chain (RC) complexes I, II and III. All of them harbor at least one [Fe-S] cluster and their activity is dependent on the mitochondrial [Fe-S] cluster (ISC) assembly machinery...
August 3, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/28803034/variations-in-the-costs-of-radical-cystectomy-for-bladder-cancer-in-the-usa
#10
Jeffrey J Leow, Alexander P Cole, Thomas Seisen, Joaquim Bellmunt, Matthew Mossanen, Mani Menon, Mark A Preston, Toni K Choueiri, Adam S Kibel, Benjamin I Chung, Maxine Sun, Steven L Chang, Quoc-Dien Trinh
BACKGROUND: Radical cystectomy (RC) for muscle-invasive bladder cancer (BCa) has potential for serious complications, prolonged length of stay and readmissions-all of which may increase costs. Although variations in outcomes are well described, less is known about determinants driving variation in costs. OBJECTIVE: To assess surgeon- and hospital-level variations in costs and predictors of high- and low-cost RC. DESIGN, SETTING, AND PARTICIPANTS: Cohort study of 23 173 patients who underwent RC for BCa in 208 hospitals in the USA from 2003 to 2015 in the Premier Healthcare Database...
August 9, 2017: European Urology
https://www.readbyqxmd.com/read/28802856/different-mathematical-processing-of-absorption-ratio-and-derivative-spectra-for-quantification-of-mixtures-containing-minor-component-an-application-to-the-analysis-of-the-recently-co-formulated-antidiabetic-drugs-canagliflozin-and-metformin
#11
Hayam M Lotfy, Dalia Mohamed, Mona S Elshahed
In the presented work several spectrophotometric methods were performed for the quantification of canagliflozin (CGZ) and metformin hydrochloride (MTF) simultaneously in their binary mixture. Two of these methods; response correlation (RC) and advanced balance point-spectrum subtraction (ABP-SS) were developed and introduced for the first time in this work, where the latter method (ABP-SS) was performed on both the zero order and the first derivative spectra of the drugs. Besides, two recently established methods; advanced amplitude modulation (AAM) and advanced absorbance subtraction (AAS) were also accomplished...
August 5, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28801768/the-influence-of-particle-size-and-feedstock-of-biochar-on-the-accumulation-of-cd-zn-pb-and-as-by-brassica-chinensis-l
#12
Ruilun Zheng, Cui Li, Guoxin Sun, Zubin Xie, Jie Chen, Juying Wu, Qinghai Wang
Biochar produced from rice straw (RC) and maize stalk (MC) was amended to the heavy metal-contaminated soil to investigate the effects of different biochar feedstock and particle size (fine, moderate, coarse) on the accumulation of Cd, Zn, Pb, and As in Brassica chinensis L. (Chinese cabbage). The concentrations of Cd, Zn, and Pb in shoot were decreased by up to 57, 75, and 63%, respectively, after biochar addition (4%). Only MC decreased As concentration in B. chinensis L. shoots by up to 61%. Biochar treatments significantly decreased NH4NO3-extractable concentrations of Cd, Zn, and Pb in soil by 47-62, 33-66, and 38-71%, respectively, yet increased that of As by up to 147%...
August 11, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28801444/radical-cystectomy-bladder-removal-against-intravesical-bcg-immunotherapy-for-high-risk-non-muscle-invasive-bladder-cancer-bravo-a-protocol-for-a-randomised-controlled-feasibility-study
#13
Jamie B Oughton, Heather Poad, Maureen Twiddy, Michelle Collinson, Victoria Hiley, Kathryn Gordon, Mark Johnson, Sunjay Jain, Aidan P Noon, Rohit Chahal, Matt Simms, Mohantha Dooldeniya, Phillip Koenig, Louise Goodwin, Julia M Brown, James W F Catto
INTRODUCTION: High-risk non-muscle invasive bladder cancer (HRNMIBC) is a heterogeneous disease that can be difficult to predict. While around 25% of cancers progress to invasion and metastases, the remaining majority of tumours remain within the bladder. It is uncertain whether patients with HRNMIBC are better treated with intravesical maintenance BCG (mBCG) immunotherapy or primary radical cystectomy (RC). A definitive randomised controlled trial (RCT) is needed to compare these two different treatments but may be difficult to recruit to and has not been attempted to date...
August 11, 2017: BMJ Open
https://www.readbyqxmd.com/read/28801130/prospective-implementation-of-enhanced-recovery-after-surgery-protocols-to-radical-cystectomy
#14
Karl H Pang, Ruth Groves, Suresh Venugopal, Aidan P Noon, James W F Catto
BACKGROUND: Multimodal enhanced recovery after surgery (ERAS) regimens have improved outcomes from colorectal surgery. OBJECTIVE: We report the application of ERAS to patients undergoing radical cystectomy (RC). DESIGN, SETTING, AND PARTICIPANTS: Prospective collection of outcomes from consecutive patients undergoing RC at a single institution. INTERVENTION: Twenty-six components including prehabilitation exercise, same day admission, carbohydrate fluid loading, targeted intraoperative fluid resuscitation, regional local anaesthesia, cessation of nasogastric tubes, omitting oral bowel preparation, avoiding drain use, early mobilisation, chewing gum use, and audit...
August 8, 2017: European Urology
https://www.readbyqxmd.com/read/28801049/a-four-state-parametric-model-for-the-kinetics-of-the-non-photochemical-quenching-in-photosystem-ii
#15
Joris J Snellenburg, Matthew P Johnson, Alexander V Ruban, Rienk van Grondelle, Ivo H M van Stokkum
The phenomenon of non-photochemical quenching (NPQ) was studied in spinach chloroplasts using pulse amplitude modulated (PAM) fluorometry. We present a new analysis method which describes the observed fluorescence quantum yield as the sum of the product of four different states of PsiI and their corresponding quantum yields. These four distinct states are PsiI in the quenched or unquenched state, and with its reaction center either open or closed depending upon the reduction of the QA site. With this method we can describe the dynamics of the NPQ induction and recovery as well as quantify the percentage of photoinactivated RC throughout the measurement...
August 8, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28799726/external-hyphae-of-rhizophagus-irregularis-daom-197198-are-less-sensitive-to-low-ph-than-roots-in-arbuscular-mycorrhizae-evidence-from-axenic-culture-system
#16
Ning Wang, Zengwei Feng, Yang Zhou, Honghui Zhu, Qing Yao
The growth of plant roots and arbuscular mycorrhizal fungi (AMF) can be inhibited by low pH; however, it is largely unknown which is more sensitive to low pH. This study aimed to compare the physiological and molecular responses of external hyphae (EH) and roots to low pH in terms of growth, development and functioning. We established AM symbiosis in a two-compartmented system (root compartment, RC; hyphal compartment, HC) using AMF and transformed hairy roots and exposed them to pH 6.5 and/or pH 4.5. The results showed that pH 4...
August 11, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/28799708/acute-and-mid-term-outcomes-of-stent-implantation-for-recurrent-coarctation-of-the-aorta-between-the-norwood-operation-and-fontan-completion-a-multi-center-pediatric-interventional-cardiology-early-career-society-investigation
#17
Osamah Aldoss, Bryan H Goldstein, Saar Danon, Sebastian Goreczny, Robert G Gray, Shyam Sathanandam, Wendy Whiteside, Derek A Williams, Jeffrey D Zampi
OBJECTIVES: We sought to evaluate outcomes of stent implantation (SI) for recurrent coarctation of the aorta (RC) following the Norwood operation. BACKGROUND: RC is common following the Norwood operation. Balloon angioplasty (BA) is standard treatment but may result in unsatisfactory relief of RC. SI may improve RC, but outcome data are limited. METHODS: We performed a multi-center retrospective study of patients who underwent SI for RC between the Norwood operation and Fontan completion...
August 11, 2017: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/28795982/treatment-of-patients-with-hepatitis-c-virus-infection-genotype-4-with-ledipasvir-sofosbuvir-in-the-liver-transplant-setting
#18
Faisal Abaalkhail, Hussein Elsiesy, Hany Elbeshbeshy, Mohamed Shawkat, Sarra Yousif, Waheed Ullah, Saleh Alabbad, Ahmed Al-Jedai, Aziza Ajlan, Dieter Broering, Sammy Saab, Mohammed Al Sebayel, Waleed Al-Hamoudi
BACKGROUND: Hepatitis C virus infection is a major cause of liver cirrhosis and hepatocellular carcinoma and the leading indication for liver transplantation. In the Middle East, genotype 4 HCV infection is the most common genotype. However, limited data exists on the treatment of genotype-4 in the liver transplant setting. We evaluated the safety and efficacy of ledipasvir-sofosbuvir (LDV/SOF) in treating HCV genotype-4 infected patients with cirrhosis or postliver transplantation. METHODS: This prospective, single-arm, observational study includes cohort of patients with cirrhosis before liver transplantation (Cohort A) and a cohort of postliver transplantation patients (Cohort B)...
August 9, 2017: Transplantation
https://www.readbyqxmd.com/read/28794896/prevalence-of-and-associations-with-agitation-in-residents-with-dementia-living-in-care-homes-marque-cross-sectional-study
#19
Gill Livingston, Julie Barber, Louise Marston, Penny Rapaport, Deborah Livingston, Sian Cousins, Sarah Robertson, Francesca La Frenais, Claudia Cooper
BACKGROUND: Agitation is reportedly the most common neuropsychiatric symptom in care home residents with dementia. AIMS: To report, in a large care home survey, prevalence and determinants of agitation in residents with dementia. METHOD: We interviewed staff from 86 care homes between 13 January 2014 and 12 November 2015 about residents with dementia with respect to agitation (Cohen-Mansfield Agitation Inventory (CMAI)), quality of life (DEMQOL-proxy) and dementia severity (Clinical Dementia Rating)...
July 2017: BJPsych Open
https://www.readbyqxmd.com/read/28791976/a-prospective-randomized-double-blind-clinical-trial-of-one-nano-hybrid-and-one-high-viscosity-bulk-fill-composite-restorative-systems-in-class-ii-cavities-12-months-results
#20
H Colak, U Tokay, R Uzgur, M M Hamidi, E Ercan
BACKGROUND: Recently, manufacturers have introduced bulk-fill composite resins that reportedly can be placed in increments of 4 mm or greater. OBJECTIVE: The purpose of this article was to report the results of 12 months prospective randomized clinical trial that evaluated the clinical performance of one high-viscosity bulk-fill composite resin in Class II cavities of posterior teeth. MATERIALS AND METHODS: Thirty-four participantshad at least two Class II cavities included the study...
July 2017: Nigerian Journal of Clinical Practice
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