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https://www.readbyqxmd.com/read/29159968/histochemical-and-immunohistochemical-characterization-of-rodlet-cells-in-the-intestine-of-two-teleosts-anguilla-anguilla-and-cyprinus-carpio
#1
G Bosi, J A DePasquale, M Manera, G Castaldelli, L Giari, B Sayyaf Dezfuli
Rodlet cells (RC) are characterized by a distinctive cell cortex and conspicuous inclusions named "rodlets." These cells are particularly abundant and large in size in intestine of eels. Histochemical, immunohistochemical and ultrastructural investigations were carried out on European eel Anguilla anguilla and Common carp Cyprinus carpio from Northern Italy. Eight biotinylated lectins were used to probe for specific carbohydrate residues in deparaffinized, hydrated intestinal sections of eel and carp. Five antibodies were tested on intestinal sections of both fish species: inducible nitric oxide synthase (i-NOS), leu-enkephalin, lysozyme, serotonin and tumour necrosis factor-α...
November 21, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/29159684/early-experience-with-a-new-concept-of-angioplasty-nitinol-constrained-balloon-catheter-chocolate-%C3%A2-in-severely-claudicant-patients
#2
Pasqualino Sirignano, Wassim Mansour, Alessandro d'Adamo, Simone Cuozzo, Laura Capoccia, Francesco Speziale
BACKGROUND: To report our experience in treating severely claudicant patients, employing a "nitinol-constrained" balloon (Chocolate, TriReme Medical Inc., Pleasanton, CA-USA) before drug-coated balloon (DCB) in a standardized protocol. METHODS: Eighty-one (84 limbs) consecutive Rutherford category (RC) 3 patients treated between December 2014 and December 2016 for superficial femoral artery (SFA) and popliteal arterial (PA) disease by nitinol-constrained balloon followed by DCB were enrolled...
November 20, 2017: Cardiovascular and Interventional Radiology
https://www.readbyqxmd.com/read/29156782/trends-in-neoadjuvant-chemotherapy-use-and-oncological-outcomes-for-muscle-invasive-bladder-cancer-in-japan-a-multicenter-study
#3
Go Anan, Shingo Hatakeyama, Naoki Fujita, Hiromichi Iwamura, Toshikazu Tanaka, Hayato Yamamoto, Yuki Tobisawa, Tohru Yoneyama, Takahiro Yoneyama, Yasuhiro Hashimoto, Takuya Koie, Hiroyuki Ito, Kazuaki Yoshikawa, Toshiaki Kawaguchi, Makoto Sato, Chikara Ohyama
Objective: Despite benefits of neoadjuvant chemotherapy (NAC), the adoption of guideline recommendations for NAC use in patients with muscle-invasive bladder cancer (MIBC) has been slow. We aimed to evaluate temporal trends in NAC use and oncological outcomes in a representative cohort of patients with MIBC. Methods: We included 532 patients from 4 hospitals who underwent radical cystectomy (RC) for ≥ cT2 MIBC in 1996-2017. We retrospectively evaluated temporal changes in NAC use and progression-free and overall survival...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156347/creation-of-a-3mn-1fe-cluster-in-the-oxygen-evolving-complex-of-photosystem-ii-and-investigation-of-its-functional-activity
#4
B К Semin, L N Davletshina, M Seibert, A B Rubin
Extraction of Mn cations from the oxygen-evolving complex (OEC) of Ca-depleted PSII membranes (PSII[-Ca,4Mn]) by reductants like hydroquinone (H2Q) occurs with lower efficiency at acidic pH (2Mn/reaction center [RC] are extracted at pH5.7) than at neutral pH (3Mn/RC are extracted at pH6.5) [Semin et al. Photosynth. Res. 125 (2015) 95]. Fe(II) also extracts Mn cations from PSII(-Ca,4Mn), but only 2Mn/RC at pH6.5, forming a heteronuclear 2Mn/2Fe cluster [Semin and Seibert, J. Bioenerg. Biomembr. 48 (2016) 227]...
November 11, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29156237/skeleton-skin-structured-rgo-cnts-pani-composite-fiber-electrodes-with-excellent-mechanical-and-electrochemical-performance-for-all-solid-state-symmetric-supercapacitors
#5
Dong Liu, Pengcheng Du, Wenli Wei, Hongxing Wang, Qi Wang, Peng Liu
Polyaniline coated reduced graphene oxide/carbon nanotube composite fibers ((RGO/CNTs)@PANI, RCP) with skeleton/skin structure are designed as fiber-shaped electrodes for high performance all-solid-state symmetric supercapacitor. The one-dimensional reduced graphene oxide/carbon nanotube composite fibers (RGO/CNTs, RC) are prepared via a simple in-situ reduction of graphene oxide in presence of carbon nanotubes in quartz glass pipes, which exhibit excellent mechanical performance of >193.4 MPa of tensile strength...
November 10, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29155597/aggregation-and-rheological-behavior-of-the-lavender-oil-pluronic-p123-microemulsions-in-water-ethanol-mixed-solvents
#6
Oyais Ahmad Chat, Nighat Nazir, Parvaiz Ahmad Bhat, Puthusserickal Abdulrahiman Hassan, Vinod Kumar Aswal, Aijaz Ahmad Dar
The effect of lavender oil on aggregation characteristics of P123 in aqueous-ethanolic solutions is investigated systematically by DLS, SANS and Rheology. The solubilization capacity of the formulation increased by increasing P123 concentration. The study unveiled the importance of the short chain alcohol-ethanol, as solubilization enhancer. The apparent hydrodynamic radius (Rh) increased significantly with increase in lavender oil concentration up to maximum oil solubilization capacity of the copolymer at a particular ethanol concentration...
November 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29152553/oncological-outcomes-of-sequential-intravesical-gemcitabine-and-docetaxel-in-patients-with-non-muscle-invasive-bladder-cancer
#7
Niv Milbar, Max Kates, Meera R Chappidi, Filippo Pederzoli, Takahiro Yoshida, Alexander Sankin, Phillip M Pierorazio, Mark P Schoenberg, Trinity J Bivalacqua
Background: Bacillus Calmette-Guérin (BCG) unresponsive/relapsing patients with non-muscle invasive bladder cancer (NMIBC) who prefer bladder preservation over radical cystectomy (RC) or those who do not qualify for surgery may be offered intravesical therapies. Gemcitabine (GEM) combined with Docetaxel (DOCE) has been offered at Johns Hopkins Hospital (JHH). Objective: To evaluate experience with GEM/DOCE, to confirm safety of the regimen, to identify populations that may benefit most, and to consider the appropriate endpoints for judging efficacy of second line therapies...
October 27, 2017: Bladder Cancer
https://www.readbyqxmd.com/read/29152549/systematic-review-and-meta-analysis-on-the-efficacy-of-chemotherapy-with-transurethral-resection-of-bladder-tumors-as-definitive-therapy-for-muscle-invasive-bladder-cancer
#8
George W Moran, Gen Li, Dennis J Robins, Justin T Matulay, James M McKiernan, Christopher B Anderson
Background: Bladder-sparing treatment of muscle invasive bladder cancer (MIBC) with systemic chemotherapy plus transurethral resection of bladder tumors (TURBT) is increasingly seen in the literature -both in case series and subanalyses of patients who opt out of or are unfit for radical cystectomy (RC). Survival outcomes among these patients are often impressive, but these are typically small retrospective studies from single institutions and therefore of limited clinical value. Objectives: Our aim is to summarize the literature regarding definitive treatment of MIBC with systemic chemotherapy plus TURBT and provide a meta-analysis of survival outcomes for patients who received this treatment...
October 27, 2017: Bladder Cancer
https://www.readbyqxmd.com/read/29151942/gender-related-outcome-in-bladder-cancer-patients-undergoing-radical-cystectomy
#9
Renate Pichler, Josef Fritz, Isabel Heidegger, Wilhelm Oberaigner, Wolfgang Horninger, Margarethe Hochleitner
Background: The impact of gender on oncological outcome after radical cystectomy (RC) is not fully understood yet. The aim of the study was to evaluate gender-related differences in histopathological parameters and prognosis of patients with bladder cancer undergoing RC. Methods: A retrospective analysis of a 10-year single-center cystectomy database was performed. Kaplan-Meier survival and Cox-regression analyses with sex-specific interactions were performed to determine the impact of gender on recurrence-free survival (RFS), cancer-specific survival (CSS), and overall survival (OS), in addition to established clinicopathological factors...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/29147759/patterns-and-predictors-of-recurrence-after-open-radical-cystectomy-for-bladder-cancer-a-comprehensive-review-of-the-literature
#10
REVIEW
Andrea Mari, Riccardo Campi, Riccardo Tellini, Giorgio Gandaglia, Simone Albisinni, Mohammad Abufaraj, Georgios Hatzichristodoulou, Francesco Montorsi, Roland van Velthoven, Marco Carini, Andrea Minervini, Shahrokh F Shariat
PURPOSE: To review the currently available literature reporting the patterns of recurrence and their predictive factors after open radical cystectomy (RC) for bladder cancer. METHODS: A review of the literature was performed using the MEDLINE, Scopus and Web of Sciences databases from January 1997 to May 2017. The PRISMA guidelines were followed for the conduct of the study. RESULTS: Local recurrence rate ranges between 30 and 54%. Distant recurrence is not often standardized and is reported in up to 50% of cases...
November 16, 2017: World Journal of Urology
https://www.readbyqxmd.com/read/29146510/relative-contributions-of-the-midcarpal-and-radiocarpal-joints-to-dart-thrower-s-motion-at-the-wrist
#11
Patrick M Kane, Bryan G Vopat, P Kaveh Mansuripur, Michael P Gaspar, Scott W Wolfe, Joseph J Crisco, Christopher Got
PURPOSE: To identify the relative contributions of the radiocarpal (RC) and midcarpal (MC) joints to dart-thrower's motion (DTM) of the wrist. METHODS: Six cadaveric upper extremities were fixed to a custom-designed loading jig allowing for pure moment-rotation analysis in 24 different directions of wrist motion. Each specimen was tested in 3 states: intact, simulated radiocarpal fusion (sRCF) and simulated pancarpal fusion (sPCF). Moments of ± 1.5 Nm were applied at each of 24 directions for each state and the resulting wrist rotation recorded...
November 14, 2017: Journal of Hand Surgery
https://www.readbyqxmd.com/read/29143652/genetic-profiles-of-ten-dirofilaria-immitis-isolates-susceptible-or-resistant-to-macrocyclic-lactone-heartworm-preventives
#12
Catherine Bourguinat, Kathy Keller, Jianguo Xia, Pierre Lepage, Tom L McTier, Debra J Woods, Roger K Prichard
BACKGROUND: For dogs and cats, chemoprophylaxis with macrocyclic lactone (ML) preventives for heartworm disease is widely used in the United States and other countries. Since 2005, cases of loss of efficacy (LOE) of heartworm preventives have been reported in the U.S. More recently, ML-resistant D. immitis isolates were confirmed. Previous work identified 42 genetic markers that could predict ML response in individual samples. For field surveillance, it would be more appropriate to work on microfilarial pools from individual dogs with a smaller subset of genetic markers...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29143324/bioelectrical-impedance-as-a-technique-for-estimating-postmortem-interval
#13
Eriek S Hansen, Christiane Baigent, Sophia I Reck, Melissa Connor
Estimation of postmortem interval (PMI) is a critical component of death investigation. A cadaver can be hypothesized to be a resistor-capacitor (RC) circuit the impedance (Z) of which changes in a quantifiable manner as the cadaver decomposes. This hypothesis was tested using bioelectrical impedance analysis (BIA) equipment to apply a current with a fixed amplitude at a single frequency to four cadavers over time and measuring two components of Z, resistance (R) and reactance (Xc ). Quadratic regression analysis between Z and accumulated degree days (ADD) showed a statistically significant parabolic relationship...
November 16, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/29142389/bioactive-constituents-radical-scavenging-and-antibacterial-properties-of-the-leaves-and-stem-essential-oils-from-peperomia-pellucida-l-kunth
#14
Sunday O Okoh, Benson C Iweriebor, Omobola O Okoh, Anthony I Okoh
Background: Peperomia pellucida is an annual herbaceous ethnomedicinal plant used in the treatment of a variety of communicable and noncommunicable diseases in the Amazon region. Objective: The study aimed at profiling the bioactive constituents of the leaves and stem essential oils (LEO and SEO) of P. pellucida, their in vitro antibacterial and radical scavenging properties as probable lead constituents in the management of oxidative stress and infectious diseases...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29141511/a-multimodal-mri-study-of-recovery-of-consciousness-in-severe-traumatic-brain-injury-preliminary-results
#15
Ang Yang, Xue Hong Xiao, Zhong Hua Liu, Zhi Long Wan, Ze Yan Wang
Accurate and reliable assessment of the conscious state of patients with severe TBI is vital for their future management. The purpose of the present study is to find an effective and accurate magnetic resonance imaging (MRI) method for predicting recovery of consciousness in patients with severe traumatic brain injury (TBI). Multimodal MR techniques, including structural MRI, magnetic resonance spectroscopy (MRS), diffusion tensor imaging (DTI), were used to evaluate brain damage in 58 patients with severe TBI...
November 15, 2017: Journal of Neurotrauma
https://www.readbyqxmd.com/read/29141130/enhancement-of-l-threonine-production-through-controlling-sequential-carbon-nitrogen-ratios-during-fermentation
#16
Hyeok-Won Lee, Hee-Suk Lee, Chun-Suk Kim, Jin-Gyeom Lee, Won-Kyo Kim, Eun-Gyo Lee, Hong-Weon Lee
Controlling residual glucose concentration is important for improving productivity in L-threonine fermentation. In this study, we developed a procedure to automatically control the feeding quantity of glucose solution as a function of ammonia-water consumption rate. The feeding ratio (Rc/n) of glucose and ammonia water was pre-determined via a stoichiometric approach, based on glucose-ammonia water consumption rates. In a 5-L fermenter, 102 g/L L-threonine was obtained using our glucose-ammonia water combined feeding strategy, which was then successfully applied in a 500-L fermenter (89g/L)...
November 15, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29133595/targeted-next-gen-sequencing-for-detecting-mll-gene-fusions-in-leukemia
#17
Sadia Afrin, Christine Rc Zhang, Claus Meyer, Caedyn L Stinson, Thy Pham, Timothy Jc Bruxner, Nicola C Venn, Toby N Trahair, Rosemary Sutton, Rolf Marschalek, J Lynn Fink, Andrew S Moore
Mixed Lineage Leukemia (MLL) gene rearrangements characterize approximately 70% of infant and 10% of adult and therapy-related leukemia. Conventional clinical diagnostics, including cytogenetics and fluorescence in situ hybridization (FISH) fail to detect MLL translocation partner genes (TPGs) in many patients. Long-Distance Inverse (LDI)-PCR, the 'gold standard' technique that is used to characterize MLL breakpoints is laborious and requires a large input of genomic DNA (gDNA). To overcome the limitations of current techniques, a targeted Next-Generation Sequencing (NGS) approach that requires low RNA input was tested...
November 13, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/29129617/computed-tomographic-evaluation-of-bone-stock-in-patients-with-crowe-type-iii-developmental-dysplasia-of-the-hip-implications-for-guiding-acetabular-component-placement-using-the-high-hip-center-technique
#18
Bo Liu, Yu-Hang Gao, Lu Ding, Shu-Qiang Li, Jian-Guo Liu, Xin Qi
BACKGROUND: We evaluated acetabular cup coverage (CC) and rim contact (RC) to evaluate the bone stock above the acetabulum for guiding acetabular component placement using the high hip center technique in patients with Crowe type III developmental dysplasia of the hip. METHODS: Using hip computed tomography and image processing software, pelvises were reconstructed digitally in 20 hips with Crowe type III developmental dysplasia of the hip. Mimicked cup was placed with anteversion angles of 0° (group I), 5° (group II), and 10° (group III) respectively...
October 24, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/29128420/neoadjuvant-treatment-for-muscle-invasive-bladder-cancer-the-past-the-present-and-the-future
#19
REVIEW
Tom J N Hermans, Charlotte S Voskuilen, Michiel S van der Heijden, Bernd J Schmitz-Dräger, Wassim Kassouf, Roland Seiler, Ashish M Kamat, Petros Grivas, Anne E Kiltie, Peter C Black, Bas W G van Rhijn
BACKGROUND: Approximately half of patients who undergo radical cystectomy (RC) for muscle-invasive bladder cancer (MIBC) will succumb to metastatic disease. We summarize the evidence for neoadjuvant radiation (NAR), chemo (NAC), and immunotherapy (checkpoint inhibition) prior to RC for MIBC. MATERIALS AND METHODS: Data were obtained by a search of PubMed, ClinicalTrials.gov, and Cochrane databases for English language articles published from 1925 up to 2017. RESULTS: NAC usage has increased over the last decade, while NAR is rarely administered...
November 8, 2017: Urologic Oncology
https://www.readbyqxmd.com/read/29127452/accuracy-and-prognostic-value-of-variant-histology-and-lymphovascular-invasion-at-transurethral-resection-of-bladder
#20
Mohammad Abufaraj, Shahrokh F Shariat, Beat Foerster, Carmen Pozo, Marco Moschini, David D'Andrea, Romain Mathieu, Martin Susani, Anna K Czech, Pierre I Karakiewicz, Veronika Seebacher
OBJECTIVES: To evaluate the concordance rate of lymphovascular invasion (LVI) and variant histology (VH) of transurethral resection (TUR) with radical cystectomy (RC) specimens. Furthermore, to evaluate the value of LVI and VH at TUR for predicting non-organ confined (NOC) disease, lymph node metastasis, and survival outcomes. PATIENTS AND METHODS: Two hundred and sixty-eight patients who underwent TUR and subsequent RC were reviewed. Logistic regression analyses were performed to evaluate the association of LVI and VH with NOC and lymph node metastasis at RC...
November 11, 2017: World Journal of Urology
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