E Yaqi, Yan Lin, Guoliang Yan, Jiahe Yang, Lijie Jiao, Ren Wu, Qiuyi Yan, Yinuo Chen, Yongxiang Chen, Xinwu Yan, Hongzhu Li
Hydrogen sulfide (H2 S) is the third gas signaling molecule that has been shown to be involved in the regulating vital activities in the body, including inhibition of aging. However, it is unknown whether H2 S alleviates aging in the kidney and glomerular mesangial cells (GMCs) by modulating their mitophagy. Here, results of experiments in vivo and in vitro showed that compared with control group, the renal function of mice and GMCs viability were decreased in D-gal (D-galactose) group, while the activity of SA-β-gal and p21 expression were increased, Cyclin D1 and Klotho expressions were decreased; H2 S content and CSE expression were lower; ROS and MDA contents and mitochondrial permeability transition pore (mPTP) opening were rised; ATP production and mitochondrial membrane potential (Δψm) were reduced; Apoptotic rate, the expression of cleaved caspase-9 and -3, Cyt c, p62 and Drp1 were enhanced and the expression of Bcl-2, Mfn2, Beclin-1, LC3 II/I, PINK1 and parkin were decreased...
August 17, 2023: Biochimica et Biophysica Acta. Molecular Cell Research