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https://www.readbyqxmd.com/read/27889531/sirt1-protects-cardiac-cells-against-apoptosis-induced-by-zearalenone-or-its-metabolites-%C3%AE-and-%C3%AE-zearalenol-through-an-autophagy-dependent-pathway
#1
Intidhar Ben Salem, Manel Boussabbeh, Julie Pires Da Silva, Arnaud Guilbert, Hassen Bacha, Salwa Abid-Essefi, Christophe Lemaire
Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by several species of Fusarium in cereals and agricultural products. The major ZEN metabolites are α-zearalenol (α-ZOL) and β-zearalenol (β-ZOL). In the present study, we investigated the underlying mechanism of the toxicity induced by ZEN, α-ZOL and β-ZOL in cardiac cells (H9c2). We show that treatment with ZEN or its metabolites induces the activation of the mitochondrial pathway of apoptosis as characterized by an increase in ROS generation, a loss of mitochondrial transmembrane potential (ΔΨm) and an activation of caspases...
November 23, 2016: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/27832632/autophagy-prevents-oxidative-stress-induced-loss-of-self-renewal-capacity-and-stemness-in-human-tendon-stem-cells-by-reducing-ros-accumulation
#2
Hua Chen, Heng-An Ge, Gen-Bing Wu, Biao Cheng, Yong Lu, Chaoyin Jiang
BACKGROUND/AIMS: Tendon stem cells (TSCs) exhibit a high self-renewal capacity, multi-differentiation potential, and low immunogenicity; thus, these cells might provide a new cell source for tendon repair and regeneration. TSCs are exposed to increased oxidative stress at tendon injury sites; however, how TSCs maintain their stemness under oxidative stress is not clear. METHODS AND RESULTS: In this study, we found that H2O2 treatment increased ROS accumulation in human TSCs (hTSCs) and resulted in loss of self-renewal capacity and stemness, as reflected in reduced colony formation and proliferation, decreased expression of the stemness markers Nanog, Oct-4, NS, and SSEA-4, and impaired differentiation capability...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27809300/inhibition-of-autophagy-increased-age-ros-mediated-apoptosis-in-mesangial-cells
#3
Li Xu, Qiuling Fan, Xu Wang, Xue Zhao, Lining Wang
The aim of our study was to investigate the role of autophagy, a homeostatic process involved in the lysosomal degradation of damaged cell organelles and proteins, in regulating the survival of mesangial cells treated with advanced glycation end products (AGEs). In the present study, AGEs induced mitochondrial depolarization and led to mitochondrial-dependent apoptosis in mesangial cells, as shown by the loss of the mitochondrial membrane potential; increased Bax processing; increased Caspase-9, Caspase-3 and PARP cleavage; and decreased Bcl-2 expression...
November 3, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27784315/intraoperative-hemidiaphragm-electrical-stimulation-reduces-oxidative-stress-and-upregulates-autophagy-in-surgery-patients-undergoing-mechanical-ventilation-exploratory-study
#4
Robert T Mankowski, Shakeel Ahmed, Thomas Beaver, Marvin Dirain, Chul Han, Phillip Hess, Tomas Martin, Barbara K Smith, Shinichi Someya, Christiaan Leeuwenburgh, A Daniel Martin
BACKGROUND: Mechanical ventilation (MV) during a cardio-thoracic surgery contributes to diaphragm muscle dysfunction that impairs weaning and can lead to the ventilator- induced diaphragm dysfunction. Especially, it is critical in older adults who have lower muscle reparative capacity following MV. Reports have shown that the intraoperative intermittent hemidiaphragm electrical stimulation can maintain and/or improve post-surgery diaphragm function. In particular, from a molecular point of view, intermittent ES may reduce oxidative stress and increase regulatory autophagy levels, and therefore improve diaphragm function in animal studies...
October 26, 2016: Journal of Translational Medicine
https://www.readbyqxmd.com/read/27755961/marrubium-vulgare-ethanolic-extract-induces-proliferation-block-apoptosis-and-cytoprotective-autophagy-in-cancer-cells-in-vitro
#5
V Paunovic, M Kosic, S Djordjevic, A Zugic, N Djalinac, U Gasic, V Trajkovic, J Harhaji-Trajkovic
Marrubium vulgare is a European medicinal plant with numerous beneficial effects on human health. The aim of the study was to isolate the plant ethanolic extract (MVE) and to investigate its anti-melanoma and anti-glioma effects. MVE was prepared by the modified pharmacopoeial percolation method and characterized by UHPLC-LTQ OrbiTrap MS. MVE dose-dependently reduced viability of melanoma (B16) and glioma (U251) cells, but not peripheral blood mononuclear cells. It arrested cell cycle in S+G2/M phase, which was associated with the activation of MAP kinase p38 and up-regulation of antiproliferative genes p53, p21 and p27...
September 30, 2016: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/27713820/fibromyalgia-syndrome-metabolic-and-autophagic-processes-in-intermittent-cold-stress-mice
#6
Lisa Oezel, Hanna Then, Anna L Jung, Samir Jabari, Gabriel A Bonaterra, Thaddeus T Wissniowski, Susanne F Önel, Matthias Ocker, Kati Thieme, Ralf Kinscherf, Pietro Di Fazio
Fibromyalgia is characterized by widespread musculoskeletal pain, fatigue, and depression. The aim was to analyze potential mitochondrial dysfunction or autophagy in mice after exposure to intermittent cold stress (ICS). Muscle and liver specimens were obtained from 36 mice. Lactate dehydrogenase (LDH) activity was measured. Microtubule-associated protein light chain 3 (MAP1LC3B) and glycogen content were determined histologically; muscle ultrastructure by electron microscopy. Mitochondrial- and autophagy-related markers were analyzed by RT-qPCR and Western blotting...
October 2016: Pharmacology Research & Perspectives
https://www.readbyqxmd.com/read/27711766/cyclometallated-iridium-complexes-inducing-paraptotic-cell-death-like-natural-products-synthesis-structure-and-mechanistic-aspects
#7
Suman Kumar Tripathy, Umasankar De, Niranjan Dehury, Paltan Laha, Manas Kumar Panda, Hyung Sik Kim, Srikanta Patra
Six mononuclear Ir complexes (1-6) using polypyridyl-pyrazine based ligands (L1 and L2) and {[cp*IrCl(μ-Cl)]2 and [(ppy)2Ir(μ-Cl)]2} precursors have been synthesised and characterised. Complexes 1-5 have shown potent anticancer activity against various human cancer cell lines (MCF-7, LNCap, Ishikawa, DU145, PC3 and SKOV3) while complex 6 is found to be inactive. Flow cytometry studies have established that cellular accumulation of the complexes lies in the order 2 > 1 > 5 > 4 > 3 > 6 which is in accordance with their observed cytotoxicity...
September 27, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27707689/caveolin-3-plays-a-critical-role-in-autophagy-after-ischemia-reperfusion
#8
Adam Kassan, Uyen Pham, Quynhmy Nguyen, Melissa E Reichelt, Eunbyul Cho, Piyush M Patel, David M Roth, Brian P Head, Hemal H Patel
Autophagy is a dynamic recycling process responsible for the breakdown of misfolded proteins and damaged organelles, providing nutrients and energy for cellular renovation and homeostasis. Loss of autophagy is associated with cardiovascular diseases. Caveolin-3 (Cav-3), a muscle-specific isoform, is a structural protein within caveolae and is critical to stress adaptation in the heart. Whether Cav-3 plays a role in regulating autophagy to modulate cardiac stress responses remains unknown. In the present study, we used HL-1 cells, a cardiac muscle cell line, with stable Cav-3 knockdown (Cav-3 KD) and Cav-3 overexpression (Cav-3 OE) to study the impact of Cav-3 in regulation of autophagy...
December 1, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27679973/nur77-exacerbates-pc12-cellular-injury-in-vitro-by-aggravating-mitochondrial-impairment-and-endoplasmic-reticulum-stress
#9
Huimin Gao, Zhaoyu Chen, Yongmei Fu, Xiaoyan Yang, Ruihui Weng, Rui Wang, Jianjun Lu, Mengqiu Pan, Kunlin Jin, Chris McElroy, Beisha Tang, Ying Xia, Qing Wang
The nuclear orphan receptor, Nur77 plays important roles in neuroimflammation, apoptosis, and dopaminergic neurodegeneration. We conducted a further mechanistic investigation into the association of Nur77 with cell death. Cytosporone B (Csn-B), an agonist for Nur77, and Nur77 knockdown were adopted in the 6-hydroxydopamine (OHDA)-lesioned PC12 cells to investigate the mechanisms underlying Nur77-mediated injury. The 6-OHDA incubation caused Nur77 translocation from the nucleus to cytosol and Endoplasm reticulum (ER) and induced co-localization of Tom20/Nur77 and Protein Disulfide Isomerase (PDI)/Nur77...
September 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27666506/inhibition-of-autophagy-sensitises-cells-to-hydrogen-peroxide-induced-apoptosis-protective-effect-of-mild-thermotolerance-acquired-at-40%C3%A2-c
#10
Maureen Redza-Dutordoir, Sarah Kassis, Hou Ve, Mélanie Grondin, Diana A Averill-Bates
Various toxic compounds produce reactive oxygen species, resulting in oxidative stress that threatens cellular homeostasis. Yet, lower doses of stress can stimulate defence systems allowing cell survival, whereas intense stress activates cell death pathways such as apoptosis. Mild thermal stress (40°C, 3h) induces thermotolerance, an adaptive survival response that renders cells less sensitive to subsequent toxic stress, by activating defence systems like heat shock proteins, antioxidants, anti-apoptotic and ER-stress factors...
December 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27642051/icariside-ii-a-novel-phosphodiesterase-5-inhibitor-protects-against-h2-o2-induced-pc12-cells-death-by-inhibiting-mitochondria-mediated-autophagy
#11
Jianmei Gao, Yuanyuan Deng, Caixia Yin, Yuangui Liu, Wei Zhang, Jingshan Shi, Qihai Gong
Oxidative stress is a major cause of cellular injury in a variety of human diseases including neurodegenerative disorders. Thus, removal of excessive reactive oxygen species (ROS) or suppression of ROS generation may be effective in preventing oxidative stress-induced cell death. This study was designed to investigate the effect of icariside II (ICS II), a novel phosphodiesterase 5 inhibitor, on hydrogen peroxide (H2 O2 )-induced death of highly differentiated rat neuronal PC12 cells, and to further examine the underlying mechanisms...
September 19, 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27636016/mesenchymal-stromal-cells-deficient-in-autophagy-proteins-are-susceptible-to-oxidative-injury-and-mitochondrial-dysfunction
#12
Sailaja Ghanta, Konstantin Tsoyi, Xiaoli Liu, Kiichi Nakahira, Bonna Ith, Anna A Coronata, Laura E Fredenburgh, Joshua A Englert, Claude A Piantadosi, Augustine M K Choi, Mark A Perrella
Oxidative stress resulting from inflammatory responses that occur during acute lung injury and sepsis can initiate changes in mitochondrial function. Autophagy regulates cellular processes in the setting of acute lung injury, sepsis, and oxidative stress by modulating the immune response and facilitating turnover of damaged cellular components. We have shown that mesenchymal stromal cells (MSCs) improve survival in murine models of sepsis by also regulating the immune response. However, the effect of autophagy on MSC and MSC mitochondrial function during oxidative stress is unknown...
September 16, 2016: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/27633131/autophagy-related-proteins-are-functionally-active-in-human-spermatozoa-and-may-be-involved-in-the-regulation-of-cell-survival-and-motility
#13
I M Aparicio, J Espino, I Bejarano, A Gallardo-Soler, M L Campo, G M Salido, J A Pariente, F J Peña, J A Tapia
Macroautophagy (hereafter autophagy) is an evolutionarily highly conserved cellular process that participates in the maintenance of intracellular homeostasis through the degradation of most long-lived proteins and entire organelles. Autophagy participates in some reproductive events; however, there are not reports regarding the role of autophagy in the regulation of sperm physiology. Hence, the aim of this study was to investigate whether autophagy-related proteins are present and functionally active in human spermatozoa...
September 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27591926/cisplatin-and-resveratrol-induce-apoptosis-and-autophagy-following-oxidative-stress-in-malignant-mesothelioma-cells
#14
Yoon-Jin Lee, Gina J Lee, Sun Shin Yi, Su-Hak Heo, Cho-Rong Park, Hae-Seon Nam, Moon-Kyun Cho, Sang-Han Lee
Malignant mesothelioma (MM) is characterized by poor responsiveness to current chemotherapeutic drugs, usually owing to high resistance to apoptosis. Here, we investigated chemosensitizing effects of phytochemical resveratrol, in combination with cisplatin, on MM cells. The combination treatment of cisplatin and resveratrol (CDDP/RSV) synergistically induced apoptosis, as evidenced by typical cell morphological changes, the appearance of sub-G0/G1 peak, an increase in the Annexin V(+) cells and the cleavage of caspase-3 and PARP...
August 31, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27581524/deep-hypothermia-enhanced-autophagy-protects-pc12-cells-against-oxygen-glucose-deprivation-via-a-mitochondrial-pathway
#15
Dang Tang, Cheng Wang, Yongjun Gao, Jun Pu, Jiang Long, Wei Xu
Deep hypothermia is known for its organ-preservation properties, which is introduced into surgical operations on the brain and heart, providing both safety in stopping circulation as well as an attractive bloodless operative field. However, the molecular mechanisms have not been clearly identified. This study was undertaken to determine the influence of deep hypothermia on neural apoptosis and the potential mechanism of these effects in PC12 cells following oxygen-glucose deprivation. Deep hypothermia (18°C) was given to PC12 cells while the model of oxygen-glucose deprivation (OGD) induction for 1h...
October 6, 2016: Neuroscience Letters
https://www.readbyqxmd.com/read/27551495/resveratrol-and-stat-inhibitor-enhance-autophagy-in-ovarian-cancer-cells
#16
L-X Zhong, Y Zhang, M-L Wu, Y-N Liu, P Zhang, X-Y Chen, Q-Y Kong, J Liu, H Li
Autophagic activity reflects cellular response to drug treatment and can be regulated by STAT3 signaling. Resveratrol inhibits STAT3 activation and causes remarkable growth arrest and cell death of ovarian cancer (OC) cells. However, the autophagic status and its relevance with resveratrol's anti-OC effects remain unclear. We analyzed the states of autophagic activities, the nature of autophagosomes and the levels of autophagy-related proteins (LC-3, Beclin 1 and STAT3) in resveratrol-treated CAOV-3 and OVCAR-3 OC cells using multiple approaches...
2016: Cell Death Discovery
https://www.readbyqxmd.com/read/27527270/nkct1-purified-naja-kaouthia-protein-toxin-conjugated-gold-nanoparticles-induced-akt-mtor-inactivation-mediated-autophagic-and-caspase-3-activated-apoptotic-cell-death-in-leukemic-cell
#17
Tanmoy Bhowmik, Antony Gomes
BACKGROUND: Gold nanoparticle (GNP) and snake venom protein toxin NKCT1 was conjugated as stated earlier (Bhowmik et al., 2013). The aim of this study was to explore the caspase dependent apoptotic pathway and autophagy inducing ability of gold nanoparticles tagged snake venom protein toxin NKCT1 (GNP-NKCT1) in human leukemic U937 and K562 cell line. METHODS: GNP-NKCT1 induced apoptosis in U937 and K562 cell line were assessed through mitochondrial membrane potential assay, ROS generation assay, caspase 3 pathways and western blotting...
October 2016: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/27508931/overexpression-of-hypo-phosphorylated-i%C3%AE%C2%BAb%C3%AE-at-ser313-protects-the-heart-against-sepsis
#18
Guang-Qing Wang, Tao Tang, Zhong-Shan Wang, Ying-Ying Liu, Li Wang, Peng-Fei Luo, Zhao-Fan Xia
IκBβis an inhibitor of nuclear factor kappa B(NF-κB) and participates in the cardiac response to sepsis. However, the role of the hypo-phosphorylated form of IκBβ at Ser313, which can be detected during sepsis, is unknown. Here, we examined the effects of IκBβ with a mutation at Ser313→Ala313 on cardiac damage induced by sepsis. Transgenic (Tg) mice were generated to overexpress IκBβ, in which Ser-313 is replaced with alanine ubiquitously, in order to mimic the hypo-phosphorylated form of IκBβ...
2016: PloS One
https://www.readbyqxmd.com/read/27469193/far-infrared-radiation-protects-viability-in-a-cell-model-of-spinocerebellar-ataxia-by-preventing-polyq-protein-accumulation-and-improving-mitochondrial-function
#19
Jui-Chih Chang, Shey-Lin Wu, Fredrik Hoel, Yu-Shan Cheng, Ko-Hung Liu, Mingli Hsieh, August Hoel, Karl Johan Tronstad, Kuo-Chia Yan, Ching-Liang Hsieh, Wei-Yong Lin, Shou-Jen Kuo, Shih-Li Su, Chin-San Liu
Far infrared radiation (FIR) is currently investigated as a potential therapeutic strategy in various diseases though the mechanism is unknown. Presently, we tested if FIR mediates beneficial effects in a cell model of the neurodegenerative disease spinocerebellar ataxia type 3 (SCA3). SCA3 is caused by a mutation leading to an abnormal polyglutamine expansion (PolyQ) in ataxin-3 protein. The consequent aggregation of mutant ataxin-3 results in disruption of vital cell functions. In this study, neuroblastoma cells (SK-N-SH) was transduced to express either non-pathogenic ataxin-3-26Q or pathogenic ataxin-3-78Q proteins...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27463126/absence-of-autophagy-promotes-apoptosis-by-modulating-the-ros-dependent-rlr-signaling-pathway-in-classical-swine-fever-virus-infected-cells
#20
Jingjing Pei, Jieru Deng, Zuodong Ye, Jiaying Wang, Hongchao Gou, Wenjun Liu, Mingqiu Zhao, Ming Liao, Lin Yi, Jinding Chen
A growing number of studies have demonstrated that both macroautophagy/autophagy and apoptosis are important inner mechanisms of cell to maintain homeostasis and participate in the host response to pathogens. We have previously reported that a functional autophagy pathway is trigged by infection of classical swine fever virus (CSFV) and is required for viral replication and release in host cells. However, the interplay of autophagy and apoptosis in CSFV-infected cells has not been clarified. In the present study, we demonstrated that autophagy induction with rapamycin facilitates cellular proliferation after CSFV infection, and that autophagy inhibition by knockdown of essential autophagic proteins BECN1/Beclin 1 or MAP1LC3/LC3 (microtubule-associated protein 1 light chain 3) promotes apoptosis via fully activating both intrinsic and extrinsic mechanisms in CSFV-infected cells...
October 2, 2016: Autophagy
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