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https://www.readbyqxmd.com/read/29145064/dog-cloning-with-in%C3%A2-vivo-matured-oocytes-obtaining-using-serum-estradiol-levels-for-predicting-time-of-ovulation
#1
Minghui Zhao, Seunghoon Lee, Dong-Hoon Kim, Jingu No, Yoonseok Nam, Sun A Ock, Yeoung-Gyu Ko, Tai-Young Hur
Dog cloning using in vivo-matured oocytes has been carried out for a decade. To obtain mature oocytes, serum progesterone (P4) levels are used to evaluate ovulation. However, the accuracy of these methods is not sufficient. Thus, the aim of the present study was to verify the feasibility of serum estradiol (E2) on canine ovulation determination as assessed by the percentage of dogs yielding mature oocytes. In vivo-matured oocytes were utilized for canine somatic cell nuclear transfer (SCNT), and serum P4 and E2 levels were assessed to determine ovulation and oocyte maturation...
October 27, 2017: Theriogenology
https://www.readbyqxmd.com/read/29144407/the-effects-of-various-weather-conditions-as-a-potential-ischemic-stroke-trigger-in-dogs
#2
Kristy L Meadows, Gena M Silver
Stroke is the fifth leading cause of death in the United States, and is the leading cause of serious, long-term disability worldwide. There are at least 795,000 new or recurrent strokes each year, and approximately 85% of all stroke occurrences are ischemic. Unfortunately, companion animals are also at risk for ischemic stroke. Although the exact incidence of ischemic stroke in companion animals is unknown, some studies, and the veterinary information network (VIN), report that approximately 3% of neurological case referrals are due to a stroke...
November 16, 2017: Veterinary Sciences
https://www.readbyqxmd.com/read/29143689/examination-of-the-susceptibility-gap-in-the-treatment-of-canine-heartworm-infection
#3
REVIEW
Dwight D Bowman, Jason Drake
BACKGROUND: The "susceptibility gap" in a dog diagnosed with adult heartworms has been defined as the period of time in which some Dirofilaria immitis stages are not susceptible to treatment with either macrocyclic lactones or melarsomine dihydrochloride. This was previously defined within the American Heartworm Society guidelines as a period of about 3 months "as per product labels." It can be postulated, however, that a susceptibility gap does not exist with the combination of continued macrocyclic lactone therapy coupled with a three-dose melarsomine dihydrochloride protocol where the first intramuscular treatment is near the time of first diagnosis...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29143688/investigating-management-choices-for-canine-heartworm-disease-in-northern-mississippi
#4
Tobi N Ku
BACKGROUND: There are concerns that the chronic use of macrocyclic lactone preventives to kill adult heartworms ("soft-" or "slow-kill") may have contributed to the development of macrocyclic lactone resistance. This prospective analysis was designed to expand our understanding of currently employed treatment decisions, protocols utilized in a "slow-kill" methodology, and trends in heartworm prevention in a region with concerns about macrocyclic lactone resistance. We tested the hypothesis that practitioners underestimate the actual percentage of heartworm-positive dogs treated with "slow-kill" therapy...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29143687/prevalence-and-epidemiology-of-canine-and-feline-heartworm-infection-in-taiwan
#5
Ta-Li Lu, Jun-Yue Wong, Ta-Lun Tan, Yong-Wei Hung
BACKGROUND: Heartworm, Dirofilaria immitis, has long been recognized in Taiwanese dogs but feline heartworm infection has been largely overlooked by veterinarians and pet owners. The main goal of this study was to determine the prevalence and epidemiology of canine and feline heartworm infection in Taiwan. METHODS: Household dogs and cats were selected from 103 veterinary hospitals in 13 cities throughout Taiwan. All animals were at least 1 year old, had received no heartworm prevention for more than 1 year, and had lived in the same city for at least 1 year...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29143686/acute-phase-proteins-and-markers-of-oxidative-stress-to-assess-the-severity-of-the-pulmonary-hypertension-in-heartworm-infected-dogs
#6
Elena Carretón, José Joaquín Cerón, Silvia Martínez-Subiela, Asta Tvarijonaviciute, Alicia Caro-Vadillo, José Alberto Montoya-Alonso
BACKGROUND: Canine heartworm infection is characterized by pulmonary endarteritis and pulmonary hypertension (PH). The aim of the present study was to evaluate the relationship between PH with the concentrations of different positive (C-reactive protein [CRP] and haptoglobin [Hp]) and negative (albumin and paraoxonase-1 [PON-1]) acute phase proteins (APP), as well as the oxidative stress, by measuring glutathione peroxidase (GPx) and total antioxidant capacity (TAC) in 27 heartworm-infected dogs on Day 0 (diagnosis) and Day 120 (1 month after the last adulticide injection)...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29143669/considerations-for-using-minocycline-vs-doxycycline-for-treatment-of-canine-heartworm-disease
#7
Mark G Papich
BACKGROUND: Doxycycline has been considered the first drug of choice for treating Wolbachia, a member of the Rickettsiaceae, which has a symbiotic relationship with filarial worms, including heartworms. Wolbachia, is susceptible to tetracyclines, which have been used as adjunctive treatments for heartworm disease. Treatment with doxycycline reduces Wolbachia numbers in all stages of heartworms and improves outcomes and decreased microfilaremia in dogs treated for heartworm disease. The American Heartworm Society recommends treatment with doxycycline in dogs diagnosed with heartworm disease at a dose of 10 mg/kg twice daily for 28 days...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29143666/comparative-evaluation-of-commercially-available-point-of-care-heartworm-antigen-tests-using-well-characterized-canine-plasma-samples
#8
Lindsay A Starkey, Joy V Bowles, Mark E Payton, Byron L Blagburn
BACKGROUND: Dirofilaria immitis is a worldwide parasite that is endemic in many parts of the United States. There are many commercial assays available for the detection of D. immitis antigen, one of which was modified and has reentered the market. Our objective was to compare the recently reintroduced Witness® Heartworm (HW) Antigen test Kit (Zoetis, Florham Park, NJ) and the SNAP® Heartworm RT (IDEXX Laboratories, Inc., Westbrook, ME) to the well-based ELISA DiroChek® Heartworm Antigen Test Kit (Zoetis, Florham Park, NJ)...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29143662/heat-treatment-and-false-positive-heartworm-antigen-testing-in-ex-vivo-parasites-and-dogs-naturally-infected-by-dirofilaria-repens-and-angiostrongylus-vasorum
#9
Luigi Venco, Simone Manzocchi, Marco Genchi, Laura H Kramer
BACKGROUND: Heartworm antigen testing is considered sensitive and specific. Currently available tests are reported as detecting a glycoprotein found predominantly in the reproductive tract of the female worm and can reach specificity close to 100%. Main concerns regard sensitivity in the case of light infections, the presence of immature females or cases of all-male infections. Research and development have been aimed at increasing sensitivity. Recently, heat treatment of serum prior to antigen testing has been shown to result in an increase in positive antigen test results, presumably due to disruption of natural antigen-antibody complexes...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29143657/clinical-benefits-of-incorporating-doxycycline-into-a-canine-heartworm-treatment-protocol
#10
C Thomas Nelson, Elizabeth S Myrick, Thomas A Nelson
BACKGROUND: The objective of heartworm treatment is to improve the clinical condition of the patient and to eliminate pre-cardiac, juvenile, and adult worm stages with minimal complications. Pulmonary thromboembolisms are an inevitable consequence of worm death and can result in severe pulmonary reactions and even death of the patient. To minimize these reactions, various treatment protocols involving melarsomine, the only adulticidal drug approved by the US Food and Drug Administrations (FDA), in conjunction with macrocyclic lactone heartworm preventives and glucocorticosteroids have been advocated...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29143646/validation-of-immune-complex-dissociation-methods-for-use-with-heartworm-antigen-tests
#11
Melissa J Beall, Andrea Arguello-Marin, Jan Drexel, Jiayou Liu, Ramaswamy Chandrashekar, A Rick Alleman
BACKGROUND: Antigen testing is routinely used to diagnose canine Dirofilaria immitis infections. Immune complex dissociation (ICD) methods, which were employed in the original heartworm antigen tests to release antigen that was bound by endogenous canine antibodies, were discontinued with improvements in assay reagents. The purpose of this study was to evaluate different ICD methods for detection of heartworm antigen by microtiter plate ELISA and assess the performance in samples from pet dogs...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29143645/impact-of-heat-treatment-on-dirofilaria-immitis-antigen-detection-in-shelter-dogs
#12
Brian A DiGangi, Carly Dworkin, Jason W Stull, Jeanette O'Quin, Morgan Elser, Antoinette E Marsh, Lesli Groshong, Wendy Wolfson, Brandy Duhon, Katie Broaddus, Elise N Gingrich, Emily Swiniarski, Elizabeth A Berliner
BACKGROUND: The diagnosis and management of canine heartworm disease is a growing concern for shelter veterinarians. Although the accuracy of commercial antigen test kits has been widely studied, recent reports have renewed interest in antigen blocking as a causative factor for false "no antigen detected" results. The objectives of this study were to determine the prevalence of false "no antigen detected" results in adult dogs entering shelters in northern, southern, and western regions of the country and to identify historical and clinical risk factors for such results...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29143643/subcutaneous-dirofilariosis-dirofilaria-repens-an-infection-spreading-throughout-the-old-world
#13
REVIEW
Claudio Genchi, Laura Kramer
BACKGROUND: Two main Dirofilaria species infect dogs: D. immitis and D. repens. While D. immitis has a worldwide distribution, D. repens is currently found only in Europe, Asia, and Africa. Adult D. repens are located in subcutaneous tissues of natural hosts where they survive for long periods of time. First-stage larvae, microfilariae, circulate in the peripheral bloodstream, where they are taken up by the mosquito intermediate hosts. Infected mosquitoes then transmit infective third-stage (L3) larvae to new hosts through the blood meal...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29143642/a-diagnostic-algorithm-for-evaluating-cases-of-potential-macrocyclic-lactone-resistant-heartworm
#14
Andrew R Moorhead, Christopher C Evans, Ray M Kaplan
BACKGROUND: The emergence of macrocyclic lactone resistance in canine heartworm poses a substantial threat to what is currently the only effective, FDA-approved available method of prevention. Further study of the biotypes is necessary to understand the mechanism of resistance and evaluate novel prevention options. Identifying cases of drug-resistant infection remains problematic, however, especially when poor compliance and insufficient testing are concerns. Furthermore, a definitive demonstration of resistance requires experimental infection and treatment, which is prohibitively costly...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29143403/effect-of-distal-implant-inclination-on-dislodging-forces-of-different-locator-attachments-used-for-mandibular-overdentures-an-in-vitro-study
#15
Moustafa Abdou ELsyad, Radwa Mohsen Emera, Tarek Mohy Ashmawy
PURPOSE: To evaluate the effect of distal implant inclination on axial and nonaxial retentive forces of different Locator attachments used to retain mandibular overdentures. MATERIALS AND METHODS: Four duplicate mandibular edentulous acrylic models received 2 implants in the canine areas with 0°, 5°, 10°, and 20° distal inclinations. Experimental overdentures were connected to the implants with Locator extra light retention (Le), Locator light retention (Ll), and Locator medium retention (Lm)...
November 16, 2017: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/29143248/what-do-dogs-canis-familiaris-see-a-review-of-vision-in-dogs-and-implications-for-cognition-research
#16
Sarah-Elizabeth Byosiere, Philippe A Chouinard, Tiffani J Howell, Pauleen C Bennett
Over the last 20 years, a large amount of research has been conducted in an attempt to uncover the cognitive abilities of the domestic dog. While substantial advancements have been made, progress has been impeded by the fact that little is known about how dogs visually perceive their external environment. It is imperative that future research determines more precisely canine visual processing capabilities, particularly considering the increasing number of studies assessing cognition via paradigms requiring vision...
November 15, 2017: Psychonomic Bulletin & Review
https://www.readbyqxmd.com/read/29143141/prevalence-and-genomic-characteristics-of-canine-kobuvirus-in-southwest-china
#17
Mingxiang Li, Nan Yan, Min Wang, Bin Zhang, Hua Yue, Cheng Tang
To investigate canine kobuvirus (CaKoV) infection in southwest China, 107 fecal samples were collected from dogs with obvious diarrhea in Sichuan and Chongqing regions, China. CaKoV infection was detected in 54 diarrheic samples (50.46%) by RT-PCR targeting a partial fragment (504 bp) of the 3D gene. Comparison of these partial 3D gene sequences from 14 of these CaKoV-positive samples show 95.4%-99.0% nucleotide (nt) identity within this group, and nt identities ranging from 93.1% to 98.2% with previously reported CaKoV 3D gene sequences...
November 15, 2017: Archives of Virology
https://www.readbyqxmd.com/read/29142645/effect-of-implant-and-occlusal-load-location-on-stress-distribution-in-locator-attachments-of-mandibular-overdenture-a-finite-element-study
#18
Angel Alvarez-Arenal, Ignacio Gonzalez-Gonzalez, Hector deLlanos-Lanchares, Elena Martin-Fernandez, Aritza Brizuela-Velasco, Joseba Ellacuria-Echebarria
PURPOSE: The aim of this study is to evaluate and compare the stress distribution in Locator attachments in mandibular two-implant overdentures according to implant locations and different loading conditions. MATERIALS AND METHODS: Four three-dimensional finite element models were created, simulating two osseointegrated implants in the mandible to support two Locator attachments and an overdenture. The models simulated an overdenture with implants located in the position of the level of lateral incisors, canines, second premolars, and crossed implant...
October 2017: Journal of Advanced Prosthodontics
https://www.readbyqxmd.com/read/29142638/analysis-of-dimensions-and-shapes-of-maxillary-and-mandibular-dental-arch-in-korean-young-adults
#19
Su-Jung Park, Richard Leesungbok, Jae-Won Song, Se Hun Chang, Suk-Won Lee, Su-Jin Ahn
PURPOSE: The aim of this study was to investigate dental arch dimensions and to classify arch shape in Korean young adults. MATERIALS AND METHODS: The sample included 50 Koreans with age ranging from 24 to 32 years. Maxillary and mandibular casts were fabricated using irreversible hydrocolloid and type III dental stones. Incisor-canine distance, incisor-1(st) molar distance, incisor-2(nd) molar distance, intercanine distance, inter-1(st) molar distance, and inter-2(nd) molar distance in both the maxillary and mandibular arch were measured using a three-dimensional measuring device...
October 2017: Journal of Advanced Prosthodontics
https://www.readbyqxmd.com/read/29142306/an-exome-sequencing-based-approach-for-genome-wide-association-studies-in-the-dog
#20
Bart J G Broeckx, Thomas Derrien, Stéphanie Mottier, Valentin Wucher, Edouard Cadieu, Benoît Hédan, Céline Le Béguec, Nadine Botherel, Kerstin Lindblad-Toh, Jimmy H Saunders, Dieter Deforce, Catherine André, Luc Peelman, Christophe Hitte
Genome-wide association studies (GWAS) are widely used to identify loci associated with phenotypic traits in the domestic dog that has emerged as a model for Mendelian and complex traits. However, a disadvantage of GWAS is that it always requires subsequent fine-mapping or sequencing to pinpoint causal mutations. Here, we performed whole exome sequencing (WES) and canine high-density (cHD) SNP genotyping of 28 dogs from 3 breeds to compare the SNP and linkage disequilibrium characteristics together with the power and mapping precision of exome-guided GWAS (EG-GWAS) versus cHD-based GWAS...
November 15, 2017: Scientific Reports
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