keyword
https://read.qxmd.com/read/37810582/hierarchical-self-assembly-of-a-reflectin-derived-peptide
#1
JOURNAL ARTICLE
Ana Margarida Gonçalves Carvalho Dias, Inês Pimentel Moreira, Iana Lychko, Cátia Lopes Soares, Arianna Nurrito, Arménio Jorge Moura Barbosa, Viviane Lutz-Bueno, Raffaele Mezzenga, Ana Luísa Carvalho, Ana Sofia Pina, Ana Cecília Afonso Roque
Reflectins are a family of intrinsically disordered proteins involved in cephalopod camouflage, making them an interesting source for bioinspired optical materials. Understanding reflectin assembly into higher-order structures by standard biophysical methods enables the rational design of new materials, but it is difficult due to their low solubility. To address this challenge, we aim to understand the molecular self-assembly mechanism of reflectin's basic unit-the protopeptide sequence YMDMSGYQ-as a means to understand reflectin's assembly phenomena...
2023: Frontiers in Chemistry
https://read.qxmd.com/read/37519901/squid-leucophore-inspired-engineering-of-optically-dynamic-human-cells
#2
JOURNAL ARTICLE
Georgii Bogdanov, Atrouli Chatterjee, Nataliya Makeeva, Aleeza Farrukh, Alon A Gorodetsky
Cephalopods (e.g., squids, octopuses, and cuttlefishes) possess remarkable dynamic camouflage abilities and therefore have emerged as powerful sources of inspiration for the engineering of dynamic optical technologies. Within this context, we have focused on the development of engineered living systems that can emulate the tunable optical characteristics of some squid skin cells. Herein, we expand our ability to controllably incorporate reflectin-based structures within mammalian cells via genetic engineering methods, and demonstrate that such structures can facilitate holotomographic and standard microscopy imaging of the cells...
July 21, 2023: IScience
https://read.qxmd.com/read/37403486/voltage-calibrated-finely-tunable-protein-assembly
#3
JOURNAL ARTICLE
Yin-Chen Lin, Eloise Masquelier, Yahya Al Sabeh, Lior Sepunaru, Michael J Gordon, Daniel E Morse
Neuronally triggered phosphorylation drives the calibrated and cyclable assembly of the reflectin signal transducing proteins, resulting in their fine tuning of colours reflected from specialized skin cells in squid for camouflage and communication. In close parallel to this physiological behaviour, we demonstrate for the first time that electrochemical reduction of reflectin A1, used as a surrogate for charge neutralization by phosphorylation, triggers voltage-calibrated, proportional and cyclable control of the size of the protein's assembly...
July 2023: Journal of the Royal Society, Interface
https://read.qxmd.com/read/36890909/synthetic-peptides-for-the-precise-transportation-of-proteins-of-interests-to-selectable-subcellular-areas
#4
JOURNAL ARTICLE
Junyi Song, Chuanyang Liu, Baoshan Li, Liangcheng Liu, Ling Zeng, Zonghuang Ye, Wenjian Wu, Lingyun Zhu, Biru Hu
Proteins, as gifts from nature, provide structure, sequence, and function templates for designing biomaterials. As first reported here, one group of proteins called reflectins and derived peptides were found to present distinct intracellular distribution preferences. Taking their conserved motifs and flexible linkers as Lego bricks, a series of reflectin-derivates were designed and expressed in cells. The selective intracellular localization property leaned on an RMs (canonical conserved reflectin motifs)-replication-determined manner, suggesting that these linkers and motifs were constructional fragments and ready-to-use building blocks for synthetic design and construction...
2023: Frontiers in Bioengineering and Biotechnology
https://read.qxmd.com/read/36692450/squid-skin-cell-inspired-refractive-index-mapping-of-cells-vesicles-and-nanostructures
#5
JOURNAL ARTICLE
Atrouli Chatterjee, Preeta Pratakshya, Albert L Kwansa, Nikhil Kaimal, Andrew H Cannon, Barbara Sartori, Benedetta Marmiroli, Helen Orins, Zhijing Feng, Samantha Drake, Justin Couvrette, LeAnn Le, Sigrid Bernstorff, Yaroslava G Yingling, Alon A Gorodetsky
The fascination with the optical properties of naturally occurring systems has been driven in part by nature's ability to produce a diverse palette of vibrant colors from a relatively small number of common structural motifs. Within this context, some cephalopod species have evolved skin cells called iridophores and leucophores whose constituent ultrastructures reflect light in different ways but are composed of the same high refractive index material─a protein called reflectin. Although such natural optical systems have attracted much research interest, measuring the refractive indices of biomaterial-based structures across multiple different environments and establishing theoretical frameworks for accurately describing the obtained refractive index values has proven challenging...
January 24, 2023: ACS Biomaterials Science & Engineering
https://read.qxmd.com/read/36555320/a-mini-review-on-reflectins-from-biochemical-properties-to-bio-inspired-applications
#6
REVIEW
Junyi Song, Baoshan Li, Ling Zeng, Zonghuang Ye, Wenjian Wu, Biru Hu
Some cephalopods (squids, octopuses, and cuttlefishes) produce dynamic structural colors, for camouflage or communication. The key to this remarkable capability is one group of specialized cells called iridocytes, which contain aligned membrane-enclosed platelets of high-reflective reflectins and work as intracellular Bragg reflectors. These reflectins have unusual amino acid compositions and sequential properties, which endows them with functional characteristics: an extremely high reflective index among natural proteins and the ability to answer various environmental stimuli...
December 10, 2022: International Journal of Molecular Sciences
https://read.qxmd.com/read/36325178/stack-of-cellular-lamellae-forms-a-silvered-cortex-to-conceal-the-opaque-organ-in-a-transparent-gastropod-in-epipelagic-habitat
#7
JOURNAL ARTICLE
Daisuke Sakai, Jun Nishikawa, Hiroshi Kakiuchida, Euichi Hirose
Background: Gelatinous zooplankton in epipelagic environments often have highly transparent bodies to avoid detection by their visual predators and prey; however, the digestive systems are often exceptionally opaque even in these organisms. In a holoplanktonic gastropod, Pterotrachea coronata , the visceral nucleus is an opaque organ located at the posterior end of its alimentary system, but this organ has a mirrored surface to conceal its internal opaque tissue. Results: Our ultrastructural observation proved that the cortex of the visceral nucleus comprised a stack of thin cellular lamellae forming a Bragg reflector, and the thickness of lamellae (0...
2022: PeerJ
https://read.qxmd.com/read/36283693/gene-recruitments-and-dismissals-in-the-argonaut-genome-provide-insights-into-pelagic-lifestyle-adaptation-and-shell-like-eggcase-reacquisition
#8
JOURNAL ARTICLE
Masa Aki Yoshida, Kazuki Hirota, Junichi Imoto, Miki Okuno, Hiroyuki Tanaka, Rei Kajitani, Atsushi Toyoda, Takehiko Itoh, Kazuho Ikeo, Takenori Sasaki, Davin H E Setiamarga
The paper nautilus or greater argonaut, Argonauta argo, is a species of octopods which is characterized by its pelagic lifestyle and by the presence of a protective spiral-shaped shell-like eggcase in females. To reveal the genomic background of how the species adapted to the pelagic lifestyle and acquired its shell-like eggcase, we sequenced the draft genome of the species. The genome size was 1.1 Gb, which is the smallest among the cephalopods known to date, with the top 215 scaffolds (average length 5,064,479 bp) covering 81% (1...
October 26, 2022: Genome Biology and Evolution
https://read.qxmd.com/read/35813206/tunable-cellular-localization-and-extensive-cytoskeleton-interplay-of-reflectins
#9
JOURNAL ARTICLE
Junyi Song, Chuanyang Liu, Baoshan Li, Liangcheng Liu, Ling Zeng, Zonghuang Ye, Ting Mao, Wenjian Wu, Biru Hu
Reflectin proteins are natural copolymers consisting of repeated canonical domains. They are located in a biophotonic system called Bragg lamellae and manipulate the dynamic structural coloration of iridocytes. Their biological functions are intriguing, but the underlying mechanism is not fully understood. Reflectin A1, A2, B1, and C were found to present distinguished cyto-/nucleoplasmic localization preferences in the work. Comparable intracellular localization was reproduced by truncated reflectin variants, suggesting a conceivable evolutionary order among reflectin proteins...
2022: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/35670152/color-tunable-fluorescent-hierarchical-nanoassemblies-with-concentration-encoded-emission
#10
JOURNAL ARTICLE
Jia Kong, Wenxin Li, Shixuan Zhao, Jiaxing Zhang, Tianli Yue, Yuefei Wang, Yinqiang Xia, Zhonghong Li
Cephalopods possess a dynamic coloration behavior to change their iridescence due to the concentration-induced optical properties of chromatophores and hierarchical assembly of reflectin. However, cephalopods rarely have iridescence in the darkfield. It would be interesting to develop color-tunable fluorescent hierarchical nanoassemblies with concentration-encoded emission. Herein, to construct the bioavailable fluorophore with dynamic coloration properties, a histidine-rich peptide is designed, which can self-assemble into hierarchical nanoassemblies stabilized by hydrogen bonds and π-π stacking interactions...
June 7, 2022: Small
https://read.qxmd.com/read/35645193/at-the-intersection-of-natural-structural-coloration-and-bioengineering
#11
REVIEW
Atrouli Chatterjee
Most of us get inspired by and interact with the world around us based on visual cues such as the colors and patterns that we see. In nature, coloration takes three primary forms: pigmentary coloration, structural coloration, and bioluminescence. Typically, pigmentary and structural coloration are used by animals and plants for their survival; however, few organisms are able to capture the nearly instantaneous and visually astounding display that cephalopods (e.g., octopi, squid, and cuttlefish) exhibit. Notably, the structural coloration of these cephalopods critically relies on a unique family of proteins known as reflectins...
May 23, 2022: Biomimetics
https://read.qxmd.com/read/35476418/cephalopod-mimetic-tunable-photonic-coatings-assembled-from-quasi-monodispersed-reflectin-protein-nanoparticles
#12
JOURNAL ARTICLE
Jun Jie Loke, Shawn Hoon, Ali Miserez
The remarkable dynamic camouflage ability of cephalopods arises from precisely orchestrated structural changes within their chromatophores and iridophores photonic cells. This mesmerizing color display remains unmatched in synthetic coatings and is regulated by swelling/deswelling of reflectin protein nanoparticles, which alters platelet dimensions in iridophores to control photonic patterns according to Bragg's law. Toward mimicking the photonic response of squid's skin, reflectin proteins from Sepioteuthis lessioniana were sequenced, recombinantly expressed, and self-assembled into spherical nanoparticles by conjugating reflectin B1 with a click chemistry ligand...
May 11, 2022: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/34272457/design-and-fabrication-of-recombinant-reflectin-based-multilayer-reflectors-bio-design-engineering-and-photoisomerism-induced-wavelength-modulation
#13
JOURNAL ARTICLE
Emmanuel Wolde-Michael, Aled D Roberts, Derren J Heyes, Ahu G Dumanli, Jonny J Blaker, Eriko Takano, Nigel S Scrutton
The remarkable camouflage capabilities of cephalopods have inspired many to develop dynamic optical materials which exploit certain design principles and/or material properties from cephalopod dermal cells. Here, the angle-dependent optical properties of various single-layer reflectin thin-films on Si wafers are characterized within the UV-Vis-NIR regions. Following this, initial efforts to design, fabricate, and optically characterize a bio-inspired reflectin-based multilayer reflector is described, which was found to conserve the optical properties of single layer films but exhibit reduced angle-dependent visible reflectivity...
July 16, 2021: Scientific Reports
https://read.qxmd.com/read/34022064/natural-biopolymers-as-proton-conductors-in-bioelectronics
#14
REVIEW
Manping Jia, Jinhwan Kim, Tiffany Nguyen, Thi Duong, Marco Rolandi
Bioelectronic devices sense or deliver information at the interface between living systems and electronics by converting biological signals into electronic signals and vice-versa. Biological signals are typically carried by ions and small molecules. As such, ion conducting materials are ideal candidates in bioelectronics for an optimal interface. Among these materials, ion conducting polymers that are able to uptake water are particularly interesting because, in addition to ionic conductivity, their mechanical properties can closely match the ones of living tissue...
July 2021: Biopolymers
https://read.qxmd.com/read/33938723/long-range-proton-transport-in-films-from-a-reflectin-derived-polypeptide
#15
JOURNAL ARTICLE
Chengyi Xu, Nabin Kandel, Xin Qiao, Md Imran Khan, Preeta Pratakshya, Nadia E Tolouei, Bo Chen, Alon A Gorodetsky
Protein- and peptide-based proton conductors have been extensively studied because of their important roles in biological processes and established potential for bioelectronic device applications. However, despite much progress, the demonstration of long-range proton transport for such materials has remained relatively rare. Herein, we fabricate, electrically interrogate, and physically characterize films from a reflectin-derived polypeptide. The electrical measurements indicate that device-integrated films exhibit proton conductivities with values of ∼0...
May 12, 2021: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/33455403/growth-and-spatial-control-of-murine-neural-stem-cells-on-reflectin-films
#16
JOURNAL ARTICLE
Rylan Kautz, Long Phan, Janahan Arulmoli, Atrouli Chatterjee, Justin P Kerr, Mahan Naeim, James Long, Alex Allevato, Jessica E Leal-Cruz, LeAnn Le, Parsa Derakhshan, Francesco Tombola, Lisa A Flanagan, Alon A Gorodetsky
Stem cells have attracted significant attention due to their regenerative capabilities and their potential for the treatment of disease. Consequently, significant research effort has focused on the development of protein- and polypeptide-based materials as stem cell substrates and scaffolds. Here, we explore the ability of reflectin, a cephalopod structural protein, to support the growth of murine neural stem/progenitor cells (mNSPCs). We observe that the binding, growth, and differentiation of mNSPCs on reflectin films is comparable to that on more established protein-based materials...
March 9, 2020: ACS Biomaterials Science & Engineering
https://read.qxmd.com/read/33323484/structure-self-assembly-and-properties-of-a-truncated-reflectin-variant
#17
JOURNAL ARTICLE
Mehran J Umerani, Preeta Pratakshya, Atrouli Chatterjee, Juana A Cerna Sanchez, Ho Shin Kim, Gregor Ilc, Matic Kovačič, Christophe Magnan, Benedetta Marmiroli, Barbara Sartori, Albert L Kwansa, Helen Orins, Andrew W Bartlett, Erica M Leung, Zhijing Feng, Kyle L Naughton, Brenna Norton-Baker, Long Phan, James Long, Alex Allevato, Jessica E Leal-Cruz, Qiyin Lin, Pierre Baldi, Sigrid Bernstorff, Janez Plavec, Yaroslava G Yingling, Alon A Gorodetsky
Naturally occurring and recombinant protein-based materials are frequently employed for the study of fundamental biological processes and are often leveraged for applications in areas as diverse as electronics, optics, bioengineering, medicine, and even fashion. Within this context, unique structural proteins known as reflectins have recently attracted substantial attention due to their key roles in the fascinating color-changing capabilities of cephalopods and their technological potential as biophotonic and bioelectronic materials...
December 15, 2020: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/33259748/electrochemistry-as-a-surrogate-for-protein-phosphorylation-voltage-controlled-assembly-of-reflectin-a1
#18
JOURNAL ARTICLE
Sheng-Ping Liang, Robert Levenson, Brandon Malady, Michael J Gordon, Daniel E Morse, Lior Sepunaru
Phosphorylation is among the most widely distributed mechanisms regulating the tunable structure and function of proteins in response to neuronal, hormonal and environmental signals. We demonstrate here that the low-voltage electrochemical reduction of histidine residues in reflectin A1, a protein that mediates the neuronal fine-tuning of colour reflected from skin cells for camouflage and communication in squids, acts as an in vitro surrogate for phosphorylation in vivo , driving the assembly previously shown to regulate its function...
December 2020: Journal of the Royal Society, Interface
https://read.qxmd.com/read/32315669/isolation-culture-and-downstream-characterization-of-primary-microglia-and-astrocytes-from-adult-rodent-brain-and-spinal-cord
#19
JOURNAL ARTICLE
Nilesh M Agalave, Brandon T Lane, Prapti H Mody, Thomas A Szabo-Pardi, Michael D Burton
BACKGROUND: Neuroimmunologists aspire to understand the interactions between neurons, microglia, and astrocytes in the CNS. To study these cells, researchers work with either immortalized cell lines or primary cells acquired from animal tissue. Primary cells reflect in vivo characteristics and functionality compared to immortalized cells; however, they are challenging to acquire and maintain. NEW METHOD: Established protocols to harvest primary glia use neonatal rodents, here we provide a method for simultaneously isolating microglia and astrocytes from brain and/or spinal cord from adult rodents...
July 1, 2020: Journal of Neuroscience Methods
https://read.qxmd.com/read/32097553/reflectin-proteins-bind-and-reorganize-synthetic-phospholipid-vesicles
#20
JOURNAL ARTICLE
Junyi Song, Robert Levenson, Jerome Santos, Lourdes Velazquez, Fan Zhang, Deborah Fygenson, Wenjian Wu, Daniel E Morse
The reflectin proteins have been extensively studied for their role in reflectance in cephalopods. In the recently evolved Loliginid squids, these proteins and the structural color they regulate are dynamically tunable, enhancing their effectiveness for camouflage and communication. In these species, the reflectins are found in highest concentrations within the structurally tunable, membrane enclosed, periodically stacked lamellae of subcellular Bragg reflectors and in the intracellular vesicles of specialized skin cells known as iridocytes and leuocophores, respectively...
March 17, 2020: Langmuir: the ACS Journal of Surfaces and Colloids
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