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https://www.readbyqxmd.com/read/28545122/spot-a-novel-and-streamlined-microarray-platform-for-observing-cellular-trna-levels
#1
Simon Grelet, Ariel McShane, Eveline Hok, Jensen Tomberlin, Philip H Howe, Renaud Geslain
Recent studies have placed transfer RNA (tRNA), a housekeeping molecule, in the heart of fundamental cellular processes such as embryonic development and tumor progression. Such discoveries were contingent on the concomitant development of methods able to deliver high-quality tRNA profiles. The present study describes the proof of concept obtained in Escherichia coli (E. coli) for an original tRNA analysis platform named SPOt (Streamlined Platform for Observing tRNA). This approach comprises three steps. First, E...
2017: PloS One
https://www.readbyqxmd.com/read/28531970/ultrasensitive-cholesterol-biosensor-based-on-enzymatic-silver-deposition-on-gold-nanoparticles-modified-screen-printed-carbon-electrode
#2
Yong Huang, Lijie Cui, Yewei Xue, Songbai Zhang, Nixuan Zhu, Jintao Liang, Guiyin Li
Cholesterol is one of the essential structural constituents of cell membranes. Determination of cholesterol is of great importance in clinical analysis because the level of cholesterol in serum is an indicator in the diagnosis and prevention of heart diseases. In this work, a simple and ultrasensitive cholesterol biosensor based on enzymatic silver deposition was designed by immobilizing cholesterol oxidase (CHOD) and cholesterol esterase (CHER) onto the surface of gold nanoparticles (Au NPs) modified screen-printed carbon electrode (SPE)...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28527767/using-multimaterial-3-dimensional-printing%C3%A2-for-personalized-planning-of-complex-structural-heart-disease-intervention
#3
Kent Chak-Yu So, Yiting Fan, Louis Sze, Ka-Wai Kwok, Anna Kin-Yin Chan, Gary Shing-Him Cheung, Alex Pui-Wai Lee
No abstract text is available yet for this article.
May 11, 2017: JACC. Cardiovascular Interventions
https://www.readbyqxmd.com/read/28471308/fabrication-of-orientation-controlled-3d-tissues-using-a-layer-by-layer-technique-and-3d-printed-a-thermo-responsive-gel-frame
#4
Yoshinari Tsukamoto, Takami Akagi, Fumiaki Shima, Mitsuru Akashi
Herein, we report the fabrication of orientation-controlled tissues similar to heart and nerve tissues using a cell accumulation and three-dimensional (3D) printing technique. We firstly evaluated the 3D shaping ability of hydroxybutyl chitosan (HBC), a thermo-responsive polymer, by using a robotic dispensing 3D printer. HBC polymer could be laminated to a height of 1,124 ± 14 μm. Based on this result, we fabricated 3D gel frames of various shapes such as square, triangular, rectangular and circular for shape control of 3D tissue and then normal human cardiac fibroblasts (NHCFs) coated with extracellular matrix (ECM) nanofilms were seeded in the frames...
May 4, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28444177/single-centre-experience-with-transapical-transcatheter-mitral-valve-implantation%C3%A2
#5
Gry Dahle, Kjell-Arne Rein, Arnt E Fiane
OBJECTIVES: A transcatheter heart valve technique can be used in failed mitral valve repairs with annuloplasty rings, deterioriated bioprostheses and in mitral annular calcification, all serving as 'docking stations' for balloon-expandable valves. Specially designed transcatheter mitral valve platforms are used in ongoing studies for native mitral valve regurgitation. We present our single centre experience with transcatheter mitral valve implantation-transapical approach procedures in eleven patients...
April 21, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/28441847/-feasibility-of-device-closure-for-multiple-atrial-septal-defects-using-3d-printing-and-ultrasound-guided-intervention-technique
#6
X Qiu, B Lü, N Xu, C W Yan, W B Ouyang, Y Liu, F W Zhang, Z Q Yue, K J Pang, X B Pan
Objective: To investigate the feasibility of trans-catheter closure of multiple atrial septal defects (ASD) monitored by trans-thoracic echocardiography (TTE) under the guidance of 3D printing heart model. Methods: Between April and August 2016, a total of 21 patients (8 male and 13 female) with multiple ASD in Fuwai Hospital of Chinese Academy of Medical Sciences underwent CT scan and 3-dimensional echocardiography for heart disease model produced by 3D printing technique. The best occlusion program was determined through the simulation test on the model...
April 25, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28431980/shear-stress-alterations-in-the-celiac-trunk-of-patients-with-a-continuous-flow-left-ventricular-assist-device-as-shown-by-in-silico-and-in-vitro-flow-analyses
#7
Francesco Scardulla, Salvatore Pasta, Leonardo D'Acquisto, Sergio Sciacca, Valentina Agnese, Christian Vergara, Alfio Quarteroni, Francesco Clemenza, Diego Bellavia, Michele Pilato
BACKGROUND: The use of left ventricular assist devices (LVADs) to treat advanced cardiac heart failure is constantly increasing, although this device leads to high risk for gastrointestinal bleeding. METHODS: Using in-silico flow analysis, we quantified hemodynamic alterations due to continuous-flow LVAD (HeartWare, Inc., Framingham, MA) in the celiac trunk and major branches of the abdominal aorta, and then explored the relationship between wall shear stress (WSS) and celiac trunk orientation...
March 24, 2017: Journal of Heart and Lung Transplantation
https://www.readbyqxmd.com/read/28430115/a-systematic-review-of-3-d-printing-in-cardiovascular-and-cerebrovascular-diseases
#8
Zhonghua Sun, Shen Yuan Lee
OBJECTIVE: The application of 3-D printing has been increasingly used in medicine, with research showing many applications in cardiovascular disease. This systematic review analyzes those studies published about the applications of 3-D printed, patient-specific models in cardiovascular and cerebrovascular diseases. METHODS: A search of PubMed/Medline and Scopus databases was performed to identify studies investigating the 3-D printing in cardiovascular and cerebrovascular diseases...
April 10, 2017: Anatolian Journal of Cardiology
https://www.readbyqxmd.com/read/28426298/mass-media-campaign-to-reduce-consumption-of-sugar-sweetened-beverages-in-a-rural-area-of-the-united-states
#9
Thomas A Farley, Hayli S Halper, Anne M Carlin, Karen M Emmerson, Kelly N Foster, Angela R Fertig
OBJECTIVES: To evaluate a mass media campaign to reduce the consumption of sugar-sweetened beverages (SSBs). METHODS: We disseminated messages emphasizing the health risks of SSBs through television, digital channels, and local organizations over 15 weeks in 2015-2016 in the Tri-Cities region of northeast Tennessee, southwest Virginia, and southeast Kentucky. We evaluated the campaign with pre- and post-telephone surveys of adults aged 18 to 45 years in the intervention area and by examining changes in beverage sales in the intervention and a matched comparison area in western Virginia...
June 2017: American Journal of Public Health
https://www.readbyqxmd.com/read/28422483/transparent-flexible-multifunctional-nanostructured-architectures-for-non-optical-readout-proximity-and-pressure-sensing
#10
Mohit Rameshchandra Kulkarni, Rohit Abraham John, Mayank Rajput, Naveen Tiwari, Natalia Yantara, Anh Chien Nguyen, Nripan Mathews
Electronic skins need to be versatile and able to detect multiple inputs beyond simple pressure and touch while having attributes of transparency and facile manufacturability. Herein, we demonstrate a versatile nanostructured transparent sensor capable of detecting wide range of pressures and proximity as well as novel nonoptical detection of printed patterns. The architecture and fabrication processes are straightforward and show robustness to repeated cycling and testing. The sensor displays good sensitivity and stability from 30 Pa to 5 kPa without the use of microstructuration and is conformal and sensitive to be utilized as a wrist-based heart-rate monitor...
April 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28346363/rapid-and-low-cost-crp-measurement-by-integrating-a-paper-based-microfluidic-immunoassay-with-smartphone-crp-chip
#11
Meili Dong, Jiandong Wu, Zimin Ma, Hagit Peretz-Soroka, Michael Zhang, Paul Komenda, Navdeep Tangri, Yong Liu, Claudio Rigatto, Francis Lin
Traditional diagnostic tests for chronic diseases are expensive and require a specialized laboratory, therefore limiting their use for point-of-care (PoC) testing. To address this gap, we developed a method for rapid and low-cost C-reactive protein (CRP) detection from blood by integrating a paper-based microfluidic immunoassay with a smartphone (CRP-Chip). We chose CRP for this initial development because it is a strong biomarker of prognosis in chronic heart and kidney disease. The microfluidic immunoassay is realized by lateral flow and gold nanoparticle-based colorimetric detection of the target protein...
March 26, 2017: Sensors
https://www.readbyqxmd.com/read/28329105/medical-three-dimensional-printing-opens-up-new-opportunities-in-cardiology-and-cardiac-surgery
#12
Thomas Bartel, Andrew Rivard, Alejandro Jimenez, Carlos A Mestres, Silvana Müller
Advanced percutaneous and surgical procedures in structural and congenital heart disease require precise pre-procedural planning and continuous quality control. Although current imaging modalities and post-processing software assists with peri-procedural guidance, their capabilities for spatial conceptualization remain limited in two- and three-dimensional representations. In contrast, 3D printing offers not only improved visualization for procedural planning, but provides substantial information on the accuracy of surgical reconstruction and device implantations...
February 16, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28324943/overcoming-spatio-temporal-limitations-using-dynamically-scaled-in-vitro-pc-mri-a-flow-field-comparison-to-true-scale-computer-simulations-of-idealized-stented-and-patient-specific-left-main-bifurcations
#13
Susann Beier, John Ormiston, Mark Webster, John Cater, Stuart Norris, Pau Medrano-Gracia, Alistair Young, Kathleen Gilbert, Brett Cowan
The majority of patients with angina or heart failure have coronary artery disease. Left main bifurcations are particularly susceptible to pathological narrowing. Flow is a major factor of atheroma development, but limitations in imaging technology such as spatio-temporal resolution, signal-to-noise ratio (SNRv), and imaging artefacts prevent in vivo investigations. Computational fluid dynamics (CFD) modelling is a common numerical approach to study flow, but it requires a cautious and rigorous application for meaningful results...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28317159/surgical-planning-for-a-complex-double-outlet-right-ventricle-using-3d-printing
#14
Puneet Bhatla, Justin T Tretter, Sathish Chikkabyrappa, Sujata Chakravarti, Ralph S Mosca
Rapid prototyping may be beneficial in properly selected cases of complex congenital heart disease, providing detailed anatomical understanding that helps to guide potential surgical and cardiac catheterization interventions. We present a case of double-outlet right ventricle, where the decision to obtain a three-dimensional printed model helped for better understanding of the anatomy, with the additional advantage of surgical simulation in planning the surgical approach and type of surgical repair.
March 19, 2017: Echocardiography
https://www.readbyqxmd.com/read/28302738/impact-of-three-dimensional-printing-on-the-study-and-treatment-of-congenital-heart-disease
#15
Matthew Bramlet, Laura Olivieri, Kanwal Farooqi, Beth Ripley, Meghan Coakley
No abstract text is available yet for this article.
March 17, 2017: Circulation Research
https://www.readbyqxmd.com/read/28285203/flexible-heartbeat-sensor-for-wearable-device
#16
Yeon Hwa Kwak, Wonhyo Kim, Kwang Bum Park, Kunnyun Kim, Sungkyu Seo
We demonstrate a flexible strain-gauge sensor and its use in a wearable application for heart rate detection. This polymer-based strain-gauge sensor was fabricated using a double-sided fabrication method with polymer and metal, i.e., polyimide and nickel-chrome. The fabrication process for this strain-gauge sensor is compatible with the conventional flexible printed circuit board (FPCB) processes facilitating its commercialization. The fabricated sensor showed a linear relation for an applied normal force of more than 930 kPa, with a minimum detectable force of 6...
March 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28284205/usage-of-3d-models-of-tetralogy-of-fallot-for-medical-education-impact-on-learning-congenital-heart-disease
#17
RANDOMIZED CONTROLLED TRIAL
Yue-Hin Loke, Ashraf S Harahsheh, Axel Krieger, Laura J Olivieri
BACKGROUND: Congenital heart disease (CHD) is the most common human birth defect, and clinicians need to understand the anatomy to effectively care for patients with CHD. However, standard two-dimensional (2D) display methods do not adequately carry the critical spatial information to reflect CHD anatomy. Three-dimensional (3D) models may be useful in improving the understanding of CHD, without requiring a mastery of cardiac imaging. The study aimed to evaluate the impact of 3D models on how pediatric residents understand and learn about tetralogy of Fallot following a teaching session...
March 11, 2017: BMC Medical Education
https://www.readbyqxmd.com/read/28281732/utilisation-of-three-dimensional-printed-heart-models-for-operative-planning-of-complex-congenital-heart-defects
#18
Peter Olejník, Matej Nosal, Tomas Havran, Adriana Furdova, Maros Cizmar, Michal Slabej, Andrej Thurzo, Pavol Vitovic, Martin Klvac, Tibor Acel, Jozef Masura
BACKGROUND: To evaluate the accuracy of the three-dimensional printing of cardiovascular structures.To explore whether utilization of three-dimensional printed heart replicas can improve surgical and catheter interventional planning in patients with complex congenital heart defects. METHODS: Between December 2014 and November 2015, we fabricated n=8 cardiovascular models based on computed tomography data in patients with complex spatial anatomical relationships of cardiovascular structures...
March 10, 2017: Kardiologia Polska
https://www.readbyqxmd.com/read/28277924/follow-your-heart-development-of-an-evidence-based-campaign-empowering-older-women-with-hiv-to-participate-in-a-large-scale-cardiovascular-disease-prevention-trial
#19
Markella V Zanni, Kathleen Fitch, Corinne Rivard, Laura Sanchez, Pamela S Douglas, Steven Grinspoon, Laura Smeaton, Judith S Currier, Sara E Looby
BACKGROUND: Women's under-representation in HIV and cardiovascular disease (CVD) research suggests a need for novel strategies to ensure robust representation of women in HIV-associated CVD research. OBJECTIVE: To elicit perspectives on CVD research participation among a community-sample of women with or at risk for HIV, and to apply acquired insights toward the development of an evidence-based campaign empowering older women with HIV to participate in a large-scale CVD prevention trial...
March 2017: HIV Clinical Trials
https://www.readbyqxmd.com/read/28268011/hands-on-surgical-training-of-congenital-heart-surgery-using-3-dimensional-print-models
#20
Shi-Joon Yoo, Thomas Spray, Erle H Austin, Tae-Jin Yun, Glen S van Arsdell
OBJECTIVE: Patient-based congenital heart surgery (CHS) training is opportunity-based and difficult. Three-dimensional (3D) print models of the heart were used for hands-on surgical training (HOST) at the 2015 AATS and subsequently in 2 local institutions. We aim to introduce the process of 3D printing for surgical simulation and to present the attendee's responses. METHODS: Using CT or MR angiograms, the models of congenital heart disease were created and printed with flexible rubberlike material...
February 9, 2017: Journal of Thoracic and Cardiovascular Surgery
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