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https://www.readbyqxmd.com/read/28333374/odontoclastogenesis-of-mouse-papilla-derived-mdpc-23-cells-induced-by-lps
#1
C Li, W T Qi, H W Jiang
AIM: To investigate the role of LPS in the odontoclast differentiation of MDPC-23 cells. It was hypothesized that MDPC 23 odontoblast-like cells may function as odontoclasts under the influence of LPS. METHODOLOGY: MDPC-23 cells were cultured in the presence of 0.1 or 1 μg/mL LPS for 6 days. Cell viability was determined using the CCK8 assay. TRAP staining, dentine resorption assay and ROS detection by confocal laser scanning microscope were used to test the odontoclast-like function of the induced cells...
March 23, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28324845/pharmacological-effects-of-%C3%AE-d-mannuronic-acid-m2000-on-mir-146a-irak1-traf6-and-nf-%C3%AE%C2%BAb-gene-expression-as-target-molecules-in-inflammatory-reactions
#2
Seyed Shahabeddin Mortazavi Jahromi, Mehdi Malek Jamshidi, Ali Farazmand, Zahra Aghazadeh, Mehdi Yousefi, Abbas Mirshafiey
BACKGROUND: Impaired expression and function of microRNAs (miRNAs) are involved in the pathogenesis of many autoimmune and inflammatory diseases. Moreover, there is a close relationship between TLRs and miRNAs and impairment in regulating their expression which can play a vital role in the immunopathogenesis of many inflammatory reactions. This research aimed to study the pharmaceutical effects of M2000 (β-d-mannuronic acid) on the expression of miR-146a and its two target molecules (IRAK1 and TRAF6), and the transcription factor NF-κB in the HEK-Blue hTLR2 cell line...
January 24, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/28323847/role-of-the-mapks-tgf-%C3%AE-1-traf6-signaling-pathway-in-postoperative-atrial-fibrillation
#3
Daoliang Zhang, Xiaoqing Chen, Qian Wang, Shaohui Wu, Yue Zheng, Xu Liu
OBJECTIVES: To explore the relationship between the MAPKs/TGF-β1/TRAF6 signaling pathway and atrial fibrosis in patients with rheumatic heart disease (RHD) and its role in atrial fibrillation (AF) after cardiac surgery on the basis of our previous animal study of the MAPKs/TGF-β1/TRAF6 signaling pathway in atrial fibrosis. METHODS: A total of 57 patients with RHD without a history of AF consented to left atrial biopsy. Histopathology quantified the percentage of fibrosis, and real-time PCR and western blot assessed the mRNA and protein expression of TGF-β1, TRAF6, and connective tissue growth factor (CTGF), respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28323261/corrigendum-ubiquitination-of-hnrnpa1-by-traf6-links-chronic-innate-immune-signaling-with-myelodysplasia
#4
Jing Fang, Lyndsey C Bolanos, Kwangmin Choi, Xiaona Liu, Susanne Christie, Shailaja Akunuru, Rupali Kumar, Dehua Wang, Xiaoting Chen, Kenneth D Greis, Peter Stoilov, Marie-Dominique Filippi, Jaroslaw P Maciejewski, Guillermo Garcia-Manero, Matthew T Weirauch, Nathan Salamonis, Hartmut Geiger, Yi Zheng, Daniel T Starczynowski
No abstract text is available yet for this article.
March 22, 2017: Nature Immunology
https://www.readbyqxmd.com/read/28322346/resveratrol-mitigates-lipopolysaccharide-mediated-acute-inflammation-in-rats-by-inhibiting-the-tlr4-nf-%C3%AE%C2%BAbp65-mapks-signaling-cascade
#5
Guangxi Wang, Zhiqiang Hu, Qiuting Fu, Xu Song, Qiankun Cui, Renyong Jia, Yuanfeng Zou, Changliang He, Lixia Li, Zhongqiong Yin
Resveratrol (RSV) is a natural compound exhibiting anti-inflammatory effect, but the anti-inflammatory mechanism has not been fully understood. This study is aimed to evaluate the anti-inflammatory activity and mechanism of RSV in lipopolysaccharides-induced rats' model. The visceral wet/dry weight ratios and the changes of hematologic and biochemical indices indicated that LPS- stimulation mainly caused damages to liver and lung, while pretreatment with RSV could alleviate the lesions. The cytokine assays showed that RSV could markedly decrease the production of proinflammatory mediators and cytokines (IL-1, IL-1β, IL-6, NO, iNOS and COX-2), and increase the expression of anti-inflammatory mediator (IL-10)...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28319200/induction-of-immunomodulatory-mir-146a-and-mir-155-in-small-intestinal-epithelium-of-vibrio-cholerae-infected-patients-at-acute-stage-of-cholera
#6
Aziz Bitar, Rituparna De, Silvia Melgar, Kyaw Min Aung, Arman Rahman, Firdausi Qadri, Sun Nyunt Wai, Tahmina Shirin, Marie-Louise Hammarström
The potential immunomodulatory role of microRNAs in small intestine of patients with acute watery diarrhea caused by Vibrio cholerae O1 or enterotoxigenic Escherichia coli (ETEC) infection was investigated. Duodenal biopsies were obtained from study-participants at the acute (day 2) and convalescent (day 21) stages of disease, and from healthy individuals. Levels of miR-146a, miR-155 and miR-375 and target gene (IRAK1, TRAF6, CARD10) and 11 cytokine mRNAs were determined by qRT-PCR. The cellular source of microRNAs in biopsies was analyzed by in situ hybridization...
2017: PloS One
https://www.readbyqxmd.com/read/28319123/saccharomyces-boulardii-and-bacillus-subtilis-b10-modulate-tlrs-and-cytokines-expression-patterns-in-jejunum-and-ileum-of-broilers
#7
Imran Rashid Rajput, Huang Ying, Sun Yajing, Muhammad Asif Arain, Li Weifen, Li Ping, Dost Muhammad Bloch, Liu Wenhua
The present study was designed to evaluate the effects of Saccharomyces boulardii (Sb) and Bacillus subtilis B10 (Bs) on intestinal epithelial Toll like receptors (TLR), and Cytokine expression response to understand the intestinal epithelial innate immune mechanism in broilers. A total of 300 birds (Sanhuang broilers) were allotted into three groups (n = 100) and each divided into five replications (n = 20). Control group (Ctr) birds were fed basal diet, broilers in experimental groups received (1×108cfu/kg feed) Sb and Bs respectively in addition to basal diet for 72 days...
2017: PloS One
https://www.readbyqxmd.com/read/28319114/internally-tagged-ubiquitin-a-tool-to-identify-linear-polyubiquitin-modified-proteins-by-mass-spectrometry
#8
Katarzyna Kliza, Christoph Taumer, Irene Pinzuti, Mirita Franz-Wachtel, Simone Kunzelmann, Benjamin Stieglitz, Boris Macek, Koraljka Husnjak
Ubiquitination controls a plethora of cellular processes. Modifications by linear polyubiquitin have so far been linked with acquired and innate immunity, lymphocyte development and genotoxic stress response. Until now, a single E3 ligase complex (LUBAC), one specific deubiquitinase (OTULIN) and a very few linear polyubiquitinated substrates have been identified. Current methods for studying lysine-based polyubiquitination are not suitable for the detection of linear polyubiquitin-modified proteins. Here, we present an approach to discovering linear polyubiquitin-modified substrates by combining a lysine-less internally tagged ubiquitin (INT-Ub...
March 20, 2017: Nature Methods
https://www.readbyqxmd.com/read/28315393/tumor-necrosis-factor-receptor-associated-factor-6-traf6-participates-in-peroxinectin-gene-expression-in-fenneropenaeus-penicillatus
#9
Shuanghu Cai, Yucong Huang, Bei Wang, Jichang Jian, Youhou Xu
Tumor necrosis factor receptor-associated factor 6 (TRAF6) is an important cytoplasm signal adaptor that mediates signals activated by tumor necrosis factor receptor (TNFR) superfamily and the Interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily. In the study, the full-length cDNA of a TRAF6 homolog (FpTRAF6) was identified from Fenneropenaeus penicillatus. The full-length cDNA of FpTRAF6 is 2033 bp long, with an open reading frame (ORF) encoding a putative protein of 594 amino acids, including a RING type Zinc finger, two TRAF-type Zinc fingers, and a conserved C-terminal meprin and TRAF homology (MATH) domain...
March 14, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28314786/effects-of-microrna-146a-on-the-proliferation-and-apoptosis-of-human-osteoarthritis-chondrocytes-by-targeting-traf6-through-the-nf-%C3%AE%C2%BAb-signaling-pathway
#10
Jun-Hua Zhong, Jing Li, Cui-Fang Liu, Ning Liu, Rui-Xiang Bian, Shou-Mei Zhao, Shu-Yi Yan, Yong-Bing Zhang
This study aims to investigate the effects of microRNA-146a (miR-146a) on the proliferation and apoptosis of human osteoarthritis (OA) chondrocytes by targeting TRAF6 through NF-κB signaling pathway. Human normal and OA chondrocytes were selected and divided into the normal group, blank group, negative control (NC) group, miR-146a mimics group, miR-146a inhibitors, miR-146a inhibitor + si-TRAF6 group and si-TRAF6 group. Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to detect the expressions of miR-146a, TRAF6 mRNA and NF-κB mRNA...
March 17, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28297724/intraocular-delivery-of-mir-146-inhibits-diabetes-induced-retinal-functional-defects-in-diabetic-rat-model
#11
Pei Zhuang, Chithra K Muraleedharan, Shunbin Xu
Purpose: Previously, we showed that microRNA-146 (miR-146) is a pivotal negative feedback regulator of multiple nuclear factor kappa-B (NF-κB) activation pathways in retinal endothelial cells (RECs). We hypothesized that miR-146 plays an important role in diabetic retinopathy (DR) by inhibiting diabetes-induced inflammatory response in the retina. The purpose of the current study is to test this hypothesis in vivo. Methods: Lentiviruses expressing rno-miR-146a, lenti-miR-146a, and negative control oligonucleotide with scrambled sequence, lenti-miR-neg ctl, were produced...
March 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28288804/mir-146a-potentially-promotes-ivig-mediated-inhibition-of-tlr4-signaling-in-lps-activated-human-monocytes
#12
Lionel Loubaki, Dominique Chabot, Isabelle Paré, Mathieu Drouin, Renée Bazin
IVIg is used as an immunomodulatory agent in inflammatory disorders such as sepsis. IVIg also affects monocyte differentiation and functions, two processes in which microRNAs play a crucial role. Monocytes detect microorganisms through pathogen recognition receptors (PRRs) such as TLR4. MiR-146a has been shown to supress NF-κB and IRF3 activity, two key components of TLR4 signaling. To evaluate whether miR-146a is involved in the anti-inflammatory effects of IVIg, monocytes were treated with LPS or IVIg alone or, alternately, first activated with LPS followed by washing and addition of IVIg...
March 10, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28286757/effect-of-tumor-necrosis-factor-inhibitor-therapy-on-osteoclasts-precursors-in-rheumatoid-arthritis
#13
Inês P Perpétuo, Joana Caetano-Lopes, Ana Maria Rodrigues, Raquel Campanilho-Marques, Cristina Ponte, Helena Canhão, Mari Ainola, João E Fonseca
Objective. Tumor necrosis factor (TNF) increases circulating osteoclast (OC) precursors numbers by promoting their proliferation and differentiation. The aim of this study was to assess the effect of TNF inhibitors (TNFi) on the differentiation and activity of OC in rheumatoid arthritis (RA) patients. Methods. Seventeen RA patients treated with TNFi were analyzed at baseline and after a minimum follow-up period of 6 months. Blood samples were collected to assess receptor activator of nuclear factor kappa-B ligand (RANKL) surface expression on circulating leukocytes and frequency and phenotype of monocyte subpopulations...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28279663/mir-146a-mediates-inflammatory-changes-and-fibrosis-in-the-heart-in-diabetes
#14
Biao Feng, Shali Chen, Andrew Devon Gordon, Subrata Chakrabarti
Hyperglycemia induced endothelial injury is a key pathogenetic factor in diabetic cardiomyopathy. In diabetes, changes in pro-inflammatory cytokines are a key mechanism leading to cardiac fibrosis. We have previously demonstrated alteration of miR-146a in chronic diabetic complications. Here, we investigated the role of endothelial miR-146a in mediating inflammation and fibrosis in diabetic cardiomyopathy. To examine the effects of miR-146a on the inflammatory mediators, an endothelial specific miR-146a overexpressing transgenic mice (TG) using tie-2 promoter, was generated...
March 6, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28260042/protective-effect-of-naringin-against-the-lps-induced-apoptosis-of-pc12-cells-implications-for-the-treatment-of-neurodegenerative-disorders
#15
Hui Wang, You Song Xu, Miao Lin Wang, Chao Cheng, Rui Bian, Hao Yuan, Yi Wang, Ting Guo, Lin Lin Zhu, Hang Zhou
Several studies have demonstrated that increased apoptosis plays an essential role in neurodegenerative disorders. It has been demonstrated that lipopolysaccharide (LPS) induces apoptosis largely through the production of intracellular reactive oxygen species (ROS) and inflammatory mediators. In this study, we investigated the potential protective mechanisms of naringin (Nar), a pummelo peel extract, on LPS-induced PC12 cell apoptosis. Nar pre-conditioning prior to stimulation with LPS for 18 h was a prerequisite for evaluating PC12 cell viability and the protective mechanisms of Nar...
February 22, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28254499/benzo-a-pyrene-exposure-under-future-ocean-acidification-scenarios-weakens-the-immune-responses-of-blood-clam-tegillarca-granosa
#16
Wenhao Su, Shanjie Zha, Yichen Wang, Wei Shi, Guoqiang Xiao, Xueliang Chai, Hongxi Wu, Guangxu Liu
Persistent organic pollutants (POPs) are known to converge into the ocean and accumulate in the sediment, posing great threats to marine organisms such as the sessile bottom burrowing bivalves. However, the immune toxicity of POPs, such as B[a]P, under future ocean acidification scenarios remains poorly understood to date. Therefore, in the present study, the impacts of B[a]P exposure on the immune responses of a bivalve species, Tegillarca granosa, under present and future ocean acidification scenarios were investigated...
February 28, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28251965/-amphioxus-ortholog-of-ecsit-an-evolutionarily-conserved-adaptor-in-the-toll-and-bmp-signaling-pathways
#17
Y H Lin, W Zhang, J W Li, H W Zhang, D Y Chen
In vertebrates, evolutionarily conserved signaling intermediate in the Toll pathway (ECSIT) interacts with the TNF-receptor associated factor 6 (TRAF6) to regulate the processing of MEKK1, activate NF-κB, and also control BMP target genes. However, the role of ECSIT in invertebrates remains largely unexplored. We performed comparative investigations of the expression, gene structure, and phylogeny of ECSIT, Toll-like receptor (TLR), and Smad4 in the cephalochordate Branchiostoma belcheri. Phylogenetic analysis indicated that, in amphioxus, ECSIT, TLR, and Smad4 form independent clusters at the base of Chordate   clusters...
January 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28250578/oral-administration-of-resveratrol-alleviates-osteoarthritis-pathology-in-c57bl-6j-mice-model-induced-by-a-high-fat-diet
#18
Mengqi Jiang, Xingyao Li, Xiaolu Yu, Xudan Liu, Xiaolei Xu, Jianyi He, Hailun Gu, Li Liu
Obesity has been associated with osteoarthritis (OA) due to increased mass and metabolic factors which are independent of the biomechanical contribution to joint load. Resveratrol, a natural polyphenolic compound, exerts protective effects on OA through its anti-inflammatory property. However, the mechanism of resveratrol on obesity-related OA is unclear. To investigate the effect and possible mechanism of oral resveratrol on obesity-related OA, we fed C57BL/6J mice with a high-fat diet (HFD) for 16 weeks to establish obesity-related OA model; then two doses (22...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28249776/lack-of-interaction-between-between-nemo-and-sharpin-impairs-linear-ubiquitination-and-nf-%C3%AE%C2%BAb-activation-and-leads-to-incontinentia-pigmenti
#19
E Bal, E Laplantine, Y Hamel, V Dubosclard, B Boisson, A Pesacatore, C Picard, S Hadj-Rabia, G Royer, J Steffann, J P Bonnefont, V M Ursini, P Vabres, A Munnich, J L Casanova, C Bodemer, R Weil, F Agou, A Smahi
BACKGROUND: Incontinentia pigmenti (IP; MIM308300) is a severe, male-lethal, X-linked, dominant genodermatosis resulting from loss-of-function mutations in the IKBKG gene encoding NF-κB essential modulator (NEMO, the regulatory subunit of the IKK complex). In 80% of cases of IP, the deletion of exons 4 to 10 leads to the absence of NEMO and total inhibition of NF-κB signaling. Here, we described a new IKBKG mutation responsible for IP resulting in an inactive truncated form of NEMO...
February 26, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28244869/yod1-traf6-association-balances-p62-dependent-il-1-signaling-to-nf-%C3%AE%C2%BAb
#20
Gisela Schimmack, Kenji Schorpp, Kerstin Kutzner, Torben Gehring, Jara Kerstin Brenke, Kamyar Hadian, Daniel Krappmann
The ubiquitin ligase TRAF6 is a key regulator of canonical IκB kinase (IKK)/NF-κB signaling in response to interleukin-1 (IL-1) stimulation. Here, we identified the deubiquitinating enzyme YOD1 (OTUD2) as a novel interactor of TRAF6 in human cells. YOD1 binds to the C-terminal TRAF homology domain of TRAF6 that also serves as the interaction surface for the adaptor p62/Sequestosome-1, which is required for IL-1 signaling to NF-κB. We show that YOD1 competes with p62 for TRAF6 association and abolishes the sequestration of TRAF6 to cytosolic p62 aggregates by a non-catalytic mechanism...
February 28, 2017: ELife
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