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https://www.readbyqxmd.com/read/28811654/characterization-of-the-mollusc-rig-i-mavs-pathway-reveals-an-archaic-antiviral-signalling-framework-in-invertebrates
#1
Baoyu Huang, Linlin Zhang, Yishuai Du, Fei Xu, Li Li, Guofan Zhang
Despite the mitochondrial antiviral signalling protein (MAVS)-dependent RIG-I-like receptor (RLR) signalling pathway in the cytosol plays an indispensable role in the antiviral immunity of the host, surprising little is known in invertebrates. Here we characterized the major members of RLR pathway and investigated their signal transduction a Molluscs. We show that genes involved in RLR pathway were significantly induced during virus challenge, including CgRIG-I-1, CgMAVS, CgTRAF6 (TNF receptor-associated factor 6), and CgIRFs (interferon regulatory factors...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28811467/the-mtorc1-4e-bp-eif4e-axis-controls-de-novo-bcl6-protein-synthesis-in-t-cells-and-systemic-autoimmunity
#2
Woelsung Yi, Sanjay Gupta, Edd Ricker, Michela Manni, Rolf Jessberger, Yurii Chinenov, Henrik Molina, Alessandra B Pernis
Post-transcriptional modifications can control protein abundance, but the extent to which these alterations contribute to the expression of T helper (TH) lineage-defining factors is unknown. Tight regulation of Bcl6 expression, an essential transcription factor for T follicular helper (TFH) cells, is critical as aberrant TFH cell expansion is associated with autoimmune diseases, such as systemic lupus erythematosus (SLE). Here we show that lack of the SLE risk variant Def6 results in deregulation of Bcl6 protein synthesis in T cells as a result of enhanced activation of the mTORC1-4E-BP-eIF4E axis, secondary to aberrant assembly of a raptor-p62-TRAF6 complex...
August 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/28811005/regulatory-effects-of-mir-146a-b-on-the-function-of-endothelial-progenitor-cells-in-acute-ischemic-stroke-in-mice
#3
Zeng-Feng Su, Zhong-Wu Sun, Ying Zhang, Shu Wang, Qi-Gui Yu, Ze-Bing Wu
The study aims to explore how microRNA-146a/b (miR-146a/b) regulates the function of endothelial progenitor cells (EPCs) in acute ischemic stroke in mice. Eighty male SPF C57BL/6J mice were evenly divided into the model-6 h, model-12 h, model-24 h (mice suffered from middle cerebral artery occlusion [MCAO] for 6 h, 12 h and model-24 h) and normal groups. EPCs were transfected and assigned into the control, MCAO, MCAO-miR-146a, MCAO-miR-146b and MCAO-miR-146a/b groups. The qRT-PCR was used to detect miR-146a/b expression in EPCs...
August 2017: Kaohsiung Journal of Medical Sciences
https://www.readbyqxmd.com/read/28808786/inhibition-of-tlr4-alleviates-the-inflammation-and-apoptosis-of-retinal-ganglion-cells-in-high-glucose
#4
Lili Hu, Hongxia Yang, Ming Ai, Shuanghong Jiang
PURPOSE: To investigate the expression profiles of Toll-like receptor 4 (TLR4), the effect of TLR4 on inflammation, and apoptosis of retinal ganglion cells (RGCs) cultured in high glucose and the underlying mechanism. METHODS: A high-glucose model was established in RGCs isolated from Sprague-Dawley (SD) rats (2-3 days old) and identified with Brn3a. Primary cultured RGCs were divided into control (0 mM), HG1 (10 mM glucose), HG2 (20 mM glucose), HG3 (30 mM glucose), HG (20 mM glucose) + TAK-242 (1...
August 14, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28775078/tlr2-plays-a-key-role-in-platelet-hyperreactivity-and-accelerated-thrombosis-associated-with-hyperlipidemia
#5
Sudipta Biswas, Alejandro Zimman, Detao Gao, Tatiana Byzova, Eugene Podrez
Rationale: Platelet hyperreactivity, which is common in many pathological conditions, is associated with increased atherothrombotic risk. The mechanisms leading to platelet hyperreactivity are complex and not yet fully understood. Objective: Platelet hyperreactivity and accelerated thrombosis, specifically in dyslipidemia, have been mechanistically linked to accumulation in the circulation of a specific group of oxidized phospholipids (oxPCCD36) that are ligands for the platelet pattern-recognition receptor CD36...
August 3, 2017: Circulation Research
https://www.readbyqxmd.com/read/28771774/mir-146a-protects-small-intestine-against-ischemia-reperfusion-injury-by-down-regulating-tlr4-traf6-nf-%C3%AE%C2%BAb-pathway
#6
Xuemei He, Yingqiang Zheng, Shengzhi Liu, Sen Shi, Yong Liu, Yanzheng He, Chunxiang Zhang, Xiangyu Zhou
Previous studies reported that miR-146a was involved in small intestine ischemia-reperfusion (I/R) injury, but the mechanism is largely vague. Here we aimed to identify the change of miR-146a in patients with mesenteric ischemia and explore the potential regulatory mechanism of miR-146a in intestine epithelial cells survival under ischemia and I/R injury. The plasma of 20 patients with mesenteric ischemia and 25 controls was collected to examine the miR-146a expression by qPCR. Rat intestinal epithelial cells (IEC-6) and 24 male Sprague-Dawley rats were included to build ischemia and I/R model in vitro and in vivo...
August 3, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28768858/human-metapneumovirus-m2-2-protein-acts-as-a-negative-regulator-of-alpha-interferon-production-by-plasmacytoid-dendritic-cells
#7
Yoshinori Kitagawa, Madoka Sakai, Mariko Funayama, Masae Itoh, Bin Gotoh
Human metapneumovirus (HMPV) has the ability to inhibit TLR7- and TLR9-dependent alpha interferon (IFN-α) production by plasmacytoid dendritic cells (pDCs). However the inhibition mechanism remains largely unknown. To identify viral proteins responsible for this inhibition, we performed a screening of HMPV open reading frames (ORFs) for their abilities to block TLR7/9-dependent signaling reconstituted in HEK293T cells by transfection with MyD88, TRAF6, IKKα, and IRF7. This screening demonstrated that the M2-2 protein was the most potent inhibitor of TLR7/9-dependent IFN-α induction...
August 2, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28757133/corrigendum-to-autophagy-linked-fyve-containing-protein-wdfy3-interacts-with-traf6-and-modulates-rankl-induced-osteoclastogenesis-j-autoimmun-73c-2016-73-84
#8
Dennis J Wu, Ran Gu, Ritu Sarin, Regina Zavodovskaya, Chia-Pei Chen, Blaine A Christiansen, Konstantinos S Zarbalis, Iannis E Adamopoulos
No abstract text is available yet for this article.
July 27, 2017: Journal of Autoimmunity
https://www.readbyqxmd.com/read/28757040/porcine-parvovirus-infection-activates-inflammatory-cytokine-production-through-toll-like-receptor-9-and-nf-%C3%AE%C2%BAb-signaling-pathways-in-porcine-kidney-cells
#9
Yong Zhou, Xiao-Hui Jin, Ya-Xing Jing, Yue Song, Xiao-Xiang He, Lan-Lan Zheng, Ya-Bin Wang, Zhan-Yong Wei, Gai-Ping Zhang
Porcine parvovirus virus (PPV) is an animal virus that has caused high economic losses for the swine industry worldwide. Previous studies demonstrated that PPV infection induced significant production of interleukin 6 (IL-6) in vitro and in vivo. However, the inflammatory cytokines and specific signaling pathways induced during PPV infection remain largely unknown. In the present study, we analyzed the expression levels of IL-6 in PPV-infected porcine kidney 15 (PK-15) and the results showed that PPV infection induced the increase of IL-6 mRNA expression in a dose-dependent manner...
August 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28755289/downregulation-of-usp4-promotes-activation-of-microglia-and-subsequent-neuronal-inflammation-in-rat-spinal-cord-after-injury
#10
Xingjie Jiang, Mingchen Yu, Yiqing Ou, Yong Cao, Yu Yao, Ping Cai, Feng Zhang
NF-κB is involved in the activation of microglia, which induces secondary spinal cord injury (SCI). This process involves the activation of NF-κB signaling pathway by TRAF6 through its polyubiquitination function. We know that deubiquitination of TRAF6 mediated by deubiquitinating enzyme (DUB) significantly inhibits activation of NF-κB pathway. The ubiquitin-specific protease 4 (USP4) belongs to the deubiquitinase family. Therefore, we hypothesize that USP4 is involved in the microglial activation and subsequent neuronal inflammation after SCI...
July 28, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28751780/traf6-is-a-novel-ns3-interacting-protein-that-inhibits-classical-swine-fever-virus-replication
#11
Huifang Lv, Wang Dong, Zhi Cao, Xiaomeng Li, Jie Wang, Gui Qian, Qizhuang Lv, Chengbao Wang, Kangkang Guo, Yanming Zhang
Classical swine fever virus (CSFV) non-structural protein 3 (NS3) is a multifunctional non-structural protein that plays a major role in viral replication. However, how exactly NS3 exerts these functions remains unknown. Here, we identified tumour necrosis factor receptor-associated factor 6 (TRAF6) as a novel NS3-interacting protein via yeast two-hybrid analysis, co-immunoprecipitation, and glutathione S-transferase pull-down assays. Furthermore, we observed that TRAF6 overexpression significantly inhibited CSFV replication, and TRAF6 knockdown promoted CSFV replication in porcine alveolar macrophages...
July 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28750349/escherichia-coli-maltose-binding-protein-mbp-activates-mouse-th1-through-tlr2-mediated-myd88-dependent-pathway-and-tlr4-mediated-trif-dependent-pathway
#12
GuoMu Liu, YiXin Zhang, NanNan Zhang, WeiHua Ni, Jing Jie, LiNa Jiang, GuiXiang Tai
MBP (maltose-binding protein) is a component of Escherichia coli. Our previous study found that MBP directly induces the activation of Th1 (T helper type 1), but the molecular mechanism remains unclear. In the present study, CD4(+)T cells were purified from the spleens of normal mice using antibody-coated immunomagnetic beads by negative selection. CD4(+)T cells activated with a CD3/CD28 antibody were stimulated with MBP. The results indicated that MBP elevated IFN-γ mRNA levels in activated CD4(+)T cells and promoted IFN-γ production from activated CD4(+)T cells...
July 24, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28745813/subversion-of-innate-immune-responses-by-francisella-involves-the-disruption-of-traf3-and-traf6-signaling-complexes
#13
Daniela Putzova, Swarupa Panda, Anetta Härtlova, Jiří Stulík, Nelson O Gekara
The success of pathogens depends on their ability to circumvent immune defenses. Francisella tularensis is one of the most infectious bacteria known. The remarkable virulence of Francisella is believed to be due to its capacity to evade or subvert the immune system, but how remains obscure. Here we show that Francisella triggers but concomitantly inhibits the Toll-like receptor (TLR), RIG-I-like receptor (RLR) and Cytoplasmic DNA (CDR) pathways. Francisella subverts these pathways at least in part by inhibiting K63-linked polyubiquitination and assembly of TRAF6 and TRAF3 complexes that control the transcriptional responses of PRRs...
July 26, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28739486/microrna-146a-5p-attenuates-liver-fibrosis-by-suppressing-profibrogenic-effects-of-tgf%C3%AE-1-and-lipopolysaccharide
#14
Yanting Zou, Yu Cai, Di Lu, Yi Zhou, Qunyan Yao, Shuncai Zhang
Liver fibrosis is characterized by proliferation and activation of hepatic stellate cells (HSCs). Transforming growth factor-β1 (TGFβ1) is crucial for liver fibrogenesis, and gut-derived endotoxin (LPS) also plays an important role in liver fibrogenesis. In the present study, we found that microRNA-146a-5p (miR-146a-5p) could regulate TGFβ1/Smad and LPS/NF-κB/Bambi pathways to attenuate liver fibrosis. Downregulated miR-146a-5p and upregulated level of LPS were found in liver of CCl4-treated rats. On cellular level, expression of miR-146a-5p is reduced during primary rat HSCs naturally activation and changed in response to TGFβ1 and/or LPS stimulation in primary rat HSCs and human HSC line LX-2...
July 21, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28734969/integrated-analysis-of-mrna-seq-in-the-haemocytes-of-eriocheir-sinensis-in-response-to-spiroplasma-eriocheiris-infection
#15
Yinghui Wang, Yunji Xiu, Keran Bi, Jiangtao Ou, Wei Gu, Wen Wang, Qingguo Meng
The Chinese mitten crab Eriocheir sinensis is an important economic crustacean that has been exposed to various diseases. Spiroplasma eriocheiris, isolated from tremor-diseased E. sinensis, was first identified as a lethal pathogen of freshwater crustaceans. To understand the pathogenesis of S. eriocheiris to E. sinensis, the transcriptomic profiles of haemocytes in the experimental and control groups at 1 d and 7 d post-injection were obtained using Illumina HiSeq 2500. These results showed that 40,358,724, 44,462,112, 45,516,576 and 37,713,728 paired-end clean reads were obtained from the cDNA libraries of DZ1 (the control group at 1 d), DZ7 (the control group at 7 d), SY1 (the experimental group at 1 d) and SY7 (the experimental group at 7 d), respectively...
July 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28720071/developmental-expression-and-dysregulation-of-mir-146a-and-mir-155-in-down-s-syndrome-and-mouse-models-of-down-s-syndrome-and-alzheimer-s-disease
#16
A Arena, A M Iyer, I Milenkovic, G G Kovács, I Ferrer, M Perluigi, E Aronica
miR-146a and miR-155 are key regulators of the innate immune response. We hypothesized that an inflammation-mediated dysregulation of these miRNAs may occur in patients with Down syndrome (DS) and Alzheimer's disease (AD). The miRNA expression patterns were investigated by in situ hybridization in developing hippocampus from controls, patients with DS and in adults with AD pathology (DS and sporadic AD; sAD). quantitative real-time PCR was employed to evaluate the miRNA levels in the hippocampus of sAD and in mouse models of DS and AD...
July 6, 2017: Current Alzheimer Research
https://www.readbyqxmd.com/read/28708287/regulation-of-rankl-induced-osteoclastogenesis-by-ring-finger-protein-rnf114
#17
Boren Lin, Qi Ke, Douglas W Leaman, Vijay Goel, Anand Agarwal
Normal bone remodeling is a continuous process orchestrated by bone-resorbing osteoclasts and bone-forming osteoblasts, which an imbalance in bone remodeling results in metabolic bone diseases. RANKL, a member of the TNF cytokine family, functions as a key stimulator for osteoclast differentiation and maturation. Here, we report that RNF114, previously identified as a psoriasis susceptibility gene, plays a regulatory role in the RANKL/RANK/TRAF6 signaling pathway that mediates osteoclastogenesis. Our results demonstrated that RNF114 expression was significantly down-regulated in mouse osteoclast precursor cells undergoing RANKL-induced osteoclast differentiation...
July 14, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28706136/swine-irf3-irf7-attenuates-inflammatory-responses-through-tlr4-signaling-pathway
#18
Pei-Ge Chen, Yan-Jing Guan, Guang-Ming Zha, Xian-Qin Jiao, He-Shui Zhu, Cheng-Yu Zhang, Yue-Ying Wang, He-Ping Li
To explore the role of IRF3/IRF7 during inflammatory responses, we investigated the effects of swine IRF3/IRF7 on TLR4 signaling pathway and inflammatory factors expression in porcine kidney epithelial PK15 cell lines. We successfully constructed eukaryotic vectors PB-IRF3 and PB-IRF7, transfected these vectors into PK15 cells and observed GFP under a fluorescence microscope. In addition, RT-PCR was also used to detect transfection efficiency. We found that IRF3/IRF7 was efficiently overexpressed in PK15 cells...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28704394/genome-wide-host-gene-expression-analysis-in-mice-experimentally-infected-with-pasteurella-multocida
#19
G Bhuvana Priya, Viswas Konasagara Nagaleekar, A Arun Prince Milton, M Saminathan, Amod Kumar, Amit Ranjan Sahoo, Sajad Ahmad Wani, Amit Kumar, S K Gupta, Aditya P Sahoo, A K Tiwari, R K Agarwal, Ravi Kumar Gandham
Pasteurella multocida causes acute septicemic and respiratory diseases, including haemorrhagic septicaemia, in cattle and buffalo with case fatality of 100%. In the present study, mice were infected with P. multocida (1.6 × 103 cfu, intraperitoneal) to evaluate host gene expression profile at early and late stages of infection using high throughput microarray transcriptome analyses. Several differentially expressed genes (DEGs) at both the time points were identified in P.multocida infected spleen, liver and lungs...
2017: PloS One
https://www.readbyqxmd.com/read/28691814/intervention-of-dietary-dipeptide-gamma-l-glutamyl-l-valine-%C3%AE-ev-ameliorates-inflammatory-response-in-a-mouse-model-of-lps-induced-sepsis
#20
MacKenzie E Chee, Kaustav Majumder, Yoshinori Mine
Sepsis, the systemic inflammatory response syndrome (SIRS) with infection is one of the leading causes of death in critically ill patients in the developed world due to the lack of effective antisepsis treatments. This study examined the efficacy of dietary dipeptide gamma-l-glutamyl-l-valine (γ-EV), which was characterized previously as an anti-inflammatory peptide, in an LPS-induced mouse model of sepsis. BALB/c mice were administered γ-EV via oral gavage followed by an intraperitoneal injection of LPS to induce sepsis...
July 26, 2017: Journal of Agricultural and Food Chemistry
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