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https://www.readbyqxmd.com/read/28531096/the-lys-asp-tyr-triad-within-the-mite-allergen-der-p-1-propeptide-is-a-critical-structural-element-for-the-ph-dependent-initiation-of-the-protease-maturation
#1
Andy Chevigné, Vincenzo Campizi, Martyna Szpakowska, David Bourry, Marie-Eve Dumez, José C Martins, André Matagne, Moreno Galleni, Alain Jacquet
The major house dust mite allergen, Der p 1, is a papain-like cysteine protease expressed as an inactive precursor, proDer p 1, carrying an N-terminal propeptide with a unique structure. The maturation of the zymogen into an enzymatically-active form of Der p 1 is a multistep autocatalytic process initiated under acidic conditions through conformational changes of the propeptide, leading to the loss of its inhibitory ability and its subsequent gradual cleavage. The aims of this study were to characterize the residues present in the Der p 1 propeptide involved in the initiation of the zymogen maturation process, but also to assess the impact of acidic pH on the propeptide structure, the activity of Der p 1 and the fate of the propeptide...
May 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28530393/structural-factors-that-alter-the-redox-potential-of-quinones-in-cyanobacterial-and-plant-photosystem-i
#2
Keisuke Kawashima, Hiroshi Ishikita
Using the cyanobacterial and plant photosystem I (PSI) crystal structures and by considering the protonation states of all titratable residues, redox potentials (Em) of the two phylloquinones--A1A and A1B--were calculated. The calculated Em values were Em(A1A) = -773 mV and Em(A1B) = -818 mV for the plant PSI structure and Em(A1A) = -612 mV and Em(A1B) = -719 mV for the cyanobacterial PSI structure. Our analysis of the PSI crystal structures suggested that the side-chain orientations of Lys-B542 and Gln-B678 in the cyanobacterial crystal structure differ from these side-chain orientations in the plant crystal structure...
May 22, 2017: Biochemistry
https://www.readbyqxmd.com/read/28526402/outpatient-antibiotic-stewardship-interventions-and-opportunities
#3
Erica L Dobson, Michael E Klepser, Jason M Pogue, Matthew J Labreche, Alex J Adams, Timothy P Gauthier, R Brigg Turner, Christy P Su, David M Jacobs, Katie J Suda
Improving the use of antibiotics across the continuum of care is a national priority. Data outlining the misuse of antibiotics in the outpatient setting justify the expansion of antibiotic stewardship programs (ASPs) into this health care setting; however, best practices for outpatient antibiotic stewardship (AS) are not yet defined. In a companion article, we focused on recommendations to overcome challenges related to the implementation of an outpatient ASP (e.g., building the AS team and defining program metrics)...
May 16, 2017: Journal of the American Pharmacists Association: JAPhA
https://www.readbyqxmd.com/read/28526350/designing-novel-bioconjugates-of-hydroxyethylcellulose-and-salicylates-for-potential-pharmaceutical-and-pharmacological-applications
#4
Khawar Abbas, Muhammad Amin, Muhammad Ajaz Hussain, Muhammad Sher, Syed Nasir Abbas Bukhari, Ibrahim Jantan, Kevin J Edgar
The present study deals with fabrication of macromolecular prodrugs (MPDs) of salicylic acid (SA) and aspirin (ASP) based on a hydrophilic cellulose ether, hydroxyethylcellulose (HEC). Degrees of substitution (DS) of SA and ASP per HEC repeating unit (HEC-RU) were achieved ranging from 0.60-2.18 and 0.53-1.50, respectively. The amphiphilic HEC-SA conjugate 2 assembled into nanowire-like structures, while HEC-ASP conjugate 6 formed nanoparticles (diameter 300-700nm) at a water/DMSO interface. After oral administration in rabbit models, conjugates 2 and 6 showed plasma half-life of 6...
May 16, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28526311/dramatic-effects-of-a-new-antimicrobial-stewardship-program-in-a-rural-community-hospital
#5
Claudia R Libertin, Stephanie H Watson, William L Tillett, Joy H Peterson
BACKGROUND: New Joint Commission antimicrobial stewardship requirements took effect on January 1, 2017, promoted as a central strategy for coping with the emerging problems of antimicrobial resistance and Clostridium difficile infection. Our objective was to measure the effects of a new antimicrobial stewardship program (ASP) in a rural community hospital with no prior ASP, in the context of having a new infectious disease specialist on staff. METHODS: An ASP team was formed to implement a prospective audit with health care provider feedback and targeting 12 antimicrobial agents in a rural hospital in Georgia...
May 16, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28520511/upregulation-of-acref-in-quinolone-resistance-development-in-escherichia-coli-when-acrab-tolc-function-is-impaired
#6
Chuan-Zhen Zhang, Man-Xia Chang, Lei Yang, Yan-Yan Liu, Pin-Xian Chen, Hong-Xia Jiang
We studied mechanisms of drug resistance development in Escherichia coli strains lacking efflux pump components. E. coli K12 deletion mutants were subjected to increasing concentrations of ciprofloxacin (CIP) to determine the frequency of target gene mutations. We generated a series of mutants that were selected based on their minimum inhibitory concentrations (MICs) to CIP, as well as their corresponding point mutations in target genes. The mutants displayed a number of target modifications and, in particular, gyrA mutations altering codons Ser83Leu, Asp87Gly, and Asp87His as well as a change in parC at 78 (substitution of Gly for Asp)...
May 18, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28518476/adult-onset-chronic-cyclic-thrombocytopenia-in-a-rhesus-macaque-macaca-mulatta-after-dengue-virus-vaccination-and-viral-challenge
#7
Galit H Frydman, Kelly A Metcalf Pate, Robert P Marini, Armelle M de Laforcade, Irene Bosch, Vasudevan Bakthavatchalu, Sureshkumar Muthupalani, Alton G Swennes, Claire E Lyons, Ronald G Tompkins, James G Fox
An 8-year-old, male Rhesus macaque (Macaca mulatta), previously used for dengue virus (DENV) vaccine research with viral challenge, was presented with adult-onset, chronic, cyclic thrombocytopenia. Platelet number, morphology, and function were evaluated by automated hematology, peripheral blood smears, electron microscopy, flow cytometry, and impedance aggregometry. Bone marrow was evaluated by cytology. Both serum anti-dengue nonstructural protein 1 (NS1) antibodies and anti-platelet antibodies were detected by ELISA...
May 18, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28516893/design-of-electrohydrodynamic-sprayed-polyethylene-glycol-hydrogel-microspheres-for-cell-encapsulation
#8
Anisa S Qayyum, Era Jain, Grant Kolar, Yonghyun Kim, Scott A Sell, Silviya P Zustiak
Electrohydrodynamic spraying (EHS) has recently gained popularity for microencapsulation of cells for applications in cell delivery and tissue engineering. Some of the polymers compatible with EHS are alginate, chitosan, and other similar natural polymers, which are subject to ionotropic or physical gelation. It is desirable to further extend the use of the EHS technique beyond such polymers for wider biofabrication applications. Here, building upon our previous work of making PEG microspheres via EHS, we utilized the principles of EHS to fabricate cell-laden polyethylene glycol (PEG) hydrogel microspheres...
May 18, 2017: Biofabrication
https://www.readbyqxmd.com/read/28513838/the-emerging-role-of-npnt-in-tissue-injury-repair-and-bone-homeostasis
#9
REVIEW
Youqiang Sun, Vincent Kuek, Heng Qiu, Jennifer Tickner, Leilei Chen, Haibin Wang, Wei He, Jiake Xu
Nephronectin (NPNT), a highly conserved extracellular matrix protein, plays an important role in regulating cell adhesion, differentiation, spreading and survival. NPNT protein belongs to epidermal growth factor (EGF)-like superfamily and exhibits several common structural determinants; including EGF-like repeat domains, MAM domain (Meprin, A5 Protein, and Receptor Protein-Tyrosine Phosphatase µ), RGD motif (Arg-Gly-Asp) and a coiled-coil domain. It regulates integrins-mediated signaling pathways via the interaction of its RGD motif with integrin α8β1...
May 17, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28513697/self-assembled-nanohelix-from-a-bolaamphiphilic-diacetylene-via-hydrogelation-and-selective-responsiveness-towards-amino-acids-and-nucleobases
#10
Yan Meng, Jian Jiang, Minghua Liu
A bolaamphiphile with diacteylene mesogen unit and l-glutamic acid as the terminal group was newly designed and its self-assembly was investigated. The compound formed hydrogels with water upon heating and cooling. The as-formed gel could be switched to a dispersion upon mechanical shaking. Both the gel and the water dispersion undergo polymerization upon UV irradiation. However, the gel turned to blue and then purple red color, while the dispersion turned red directly. Using AFM and SEM observations, it was found that nanohelix was formed in the as-prepared gel and polymerized blue gels, while nanobelt was formed from the water dispersion...
May 17, 2017: Nanoscale
https://www.readbyqxmd.com/read/28512575/new-leads-for-fragment-based-design-of-rhenium-technetium-radiopharmaceutical-agents
#11
Alice Brink, John R Helliwell
Multiple possibilities for the coordination of fac-[Re(CO)3(H2O)3](+) to a protein have been determined and include binding to Asp, Glu, Arg and His amino-acid residues as well as to the C-terminal carboxylate in the vicinity of Leu and Pro. The large number of rhenium metal complex binding sites that have been identified on specific residues thereby allow increased target identification for the design of future radiopharmaceuticals. The core experimental concept involved the use of state-of-art tuneable synchrotron radiation at the Diamond Light Source to optimize the rhenium anomalous dispersion signal to a large value (f'' of 12...
May 1, 2017: IUCrJ
https://www.readbyqxmd.com/read/28507551/deciphering-genomic-regions-for-high-grain-iron-and-zinc-content-using-association-mapping-in-pearl-millet
#12
N Anuradha, C Tara Satyavathi, C Bharadwaj, T Nepolean, S Mukesh Sankar, Sumer P Singh, Mahesh C Meena, Tripti Singhal, Rakesh K Srivastava
Micronutrient malnutrition, especially deficiency of two mineral elements, iron [Fe] and zinc [Zn] in the developing world needs urgent attention. Pearl millet is one of the best crops with many nutritional properties and is accessible to the poor. We report findings of the first attempt to mine favorable alleles for grain iron and zinc content through association mapping in pearl millet. An association mapping panel of 130 diverse lines was evaluated at Delhi, Jodhpur and Dharwad, representing all the three pearl millet growing agro-climatic zones of India, during 2014 and 2015...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28507436/design-of-polyaspartic-acid-peptide-poly-ethylene-glycol-poly-%C3%AE%C2%B5-caprolactone-nanoparticles-as-a-carrier-of-hydrophobic-drugs-targeting-cancer-metastasized-to-bone
#13
Jinsong Liu, Youyun Zeng, Shuai Shi, Lihua Xu, Hualin Zhang, Janak L Pathak, Yihuai Pan
Treatment of cancer metastasized to bone is still a challenge due to hydrophobicity, instability, and lack of target specificity of anticancer drugs. Poly (ethylene glycol)-poly (ε-caprolactone) polymer (PEG-PCL) is an effective, biodegradable, and biocompatible hydrophobic drug carrier, but lacks bone specificity. Polyaspartic acid with eight peptide sequences, that is, (Asp)8, has a strong affinity to bone surface. The aim of this study was to synthesize (Asp)8-PEG-PCL nanoparticles as a bone-specific carrier of hydrophobic drugs to treat cancer metastasized to bone...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28506754/synthesis-biological-evaluation-and-molecular-docking-studies-of-chromone-hydrazone-derivatives-as-%C3%AE-glucosidase-inhibitors
#14
Guangcheng Wang, Ming Chen, Jing Wang, Yaping Peng, Luyao Li, ZhenZhen Xie, Bing Deng, Shan Chen, Wenbiao Li
A series of chromone hydrazone derivatives 4a-4p have been synthesized, characterized by (1)H NMR and (13)C NMR and evaluated for theirinvitro α-glucosidase inhibitory activity. Out of these tested compounds, six (4a, 4b, 4d, 4j, 4o and 4p) displayed potent α-glucosidase inhibitory activity with IC50 values in the range of 20.1±0.19μM to 45.7±0.23μM, as compared to the standard drug acarbose (IC50=817.38±6.27μM). Among this series, compound 4d (IC50=20.1±0.19μM) with 4-sulfonamide substitution at phenyl part of hydrazide was found to be the most active compound...
May 5, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28505407/unique-n-phenylacetylation-and-nrps-with-substrate-promiscuity-for-biosynthesis-of-heptapeptide-variants-jbir-78-and-jbir-95
#15
Kunpei Takeda, Kohei Kemmoku, Yasuharu Satoh, Yasushi Ogasawara, Kazuo Shin-Ya, Tohru Dairi
JBIR-78 (1) and JBIR-95 (2), both of which are heptapeptide derivatives isolated from Kibdelosporangium sp. AK-AA56, have the same amino acid sequences except for the second amino acid; phenylacetic acid (Paa)-L-Val-D-Asp (1)/D-cysteic acid (2)-L-Ala-(3S)-3-hydroxy-D-Leu-Gly-D-Ala-L-Phe. Heterologous expression of the biosynthetic gene cluster including genes encoding non-ribosomal peptide synthetases (NRPS) and in vitro assays with recombinant Orf3, L-cysteic acid synthase homolog, suggested the single A-domain in module 2 activates both L-Asp and L-cysteic acid to yield 1 and 2, respectively, although the substrate specificities of the A-domains of NRPSs are usually strict...
May 15, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28504743/accumulation-layer-surface-plasmons-in-transparent-conductive-oxides
#16
Shima Fardad, E Alexander Ramos, Alessandro Salandrino
A rigorous analytical study of the eigenmodes supported by a charge accumulation layer within a transparent conductive oxide (TCO) is presented. The new class of surface plasmons termed accumulation-layer surface plasmons (ASPs) is introduced. Near resonance ASPs are tightly bound and display a vast effective index tunability that could be of great practical interest. The suppression of ASPs in the presence of epsilon-near zero regions is discussed.
May 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28501600/development-of-a-human-epidermal-growth-factor-derivative-with-egfr-blocking-and-depleted-biological-activities-a-comparative-in-vitro-study-using-egfr-positive-breast-cancer-cells
#17
Masomeh Mehrabi, Kamran Mansouri, Bijan Soleymani, Zohreh Hoseinkhani, Mohsen Shahlaie, Reza Khodarahmi
Epidermal growth factor (EGF) is a local growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF and EGFR are involved in many aspects of the development of carcinomas. Because EGFR has been found to be over-expressed in many tumors of epithelial origin, it is a potential target for antitumor therapy. In this study we designed a mutated form of hEGF (mEGF) with a deletion of four amino acids residues (Gln(43), Tyr(44), Arg(45) and Asp(46)) in order to show importance of Leu spatial location for EGFR binding/activation...
May 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28501405/short-communication-nutrient-consumption-patterns-of-lactobacillus-acidophilus-klds-1-0738-in-controlled-ph-batch-fermentations
#18
Xuepeng Lv, Gefei Liu, Xiaomei Sun, Hongyu Chen, Jiahui Sun, Zhen Feng
This work focused on elucidating the nutrient consumption patterns of Lactobacillus acidophilus to guide the design of media for high-cell-density culture. We investigated the nutrient consumption patterns of L. acidophilus KLDS 1.0738 in chemically defined media in controlled pH batch fermentations. The most abundantly consumed amino acids, vitamins, ions, and purines and pyrimidines were Glu and Gly, pyridoxine and nicotinamide, K(+) and PO4(3-), and guanine and uracil, respectively. The highest consumption rates for amino acids, vitamins, ions, and purines and pyrimidines were Asp and Arg, folic acid and pyridoxine, Fe(2+) and Mn(2+), and uracil and thymine, respectively...
May 10, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28501176/liquid-chromatography-high-resolution-mass-spectrometric-methods-for-the-surveillance-monitoring-of-cyanotoxins-in-freshwaters
#19
Sara Bogialli, Claudio Bortolini, Iole Maria Di Gangi, Federica Nigro Di Gregorio, Luca Lucentini, Gabriella Favaro, Paolo Pastore
A comprehensive risk management on human exposure to cyanotoxins, whose production is actually unpredictable, is limited by reliable analytical tools for monitoring as many toxic algal metabolites as possible. Two analytical approaches based on a LC-QTOF system for target analysis and suspect screening of cyanotoxins in freshwater were presented. A database with 369 compounds belonging to cyanobacterial metabolites was developed and used for a retrospective data analysis based on high resolution mass spectrometry (HRMS)...
August 1, 2017: Talanta
https://www.readbyqxmd.com/read/28501104/knockdown-of-cbp-p300-interacting-transactivator-with-glu-asp-rich-carboxy-terminal-domain-2-inhibits-cell-division-and-increases-apoptosis-in-gastric-cancer
#20
Ze Tang, Gan He, Jie Xu, Li Zhongfu
BACKGROUND: Cbp/P300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (CITED2) is a pleiotropic protein associated with numerous cell functions, including transcription and differentiation. The role of CITED2 has been investigated in a number of malignancies; however, the roles of this protein in gastric cancers remain unclear. Therefore, we determined the role of CITED2 in gastric cancers. MATERIALS AND METHODS: Gastric cancer cell lines (MKN74, MKN28, 7901, and AGS) were used to assess CITED2 transcript levels...
May 1, 2017: Journal of Surgical Research
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