Kin Israel R Notarte, Mark Tristan J Quimque, Imee T Macaranas, Abbas Khan, Adriel M Pastrana, Oliver B Villaflores, Hans Christian P Arturo, Delfin Yñigo H Pilapil Iv, Sophia Morgan M Tan, Dong-Qing Wei, Arlette Wenzel-Storjohann, Deniz Tasdemir, Chia-Hung Yen, Seon Yeong Ji, Gi-Young Kim, Yung Hyun Choi, Allan Patrick G Macabeo
Pathologic hyperreactive inflammatory responses occur when there is excessive activation of a proinflammatory NF-κB pathway and a reduced cytoprotective NRF2 cascade. The noncytotoxic, highly selective COX-2 inhibitory flavonol-enriched butanol fraction (UaB) from Uvaria alba ( U. alba ) was investigated for its inflammatory modulating potential by targeting NF-κB activation and NRF2 activity. Enzyme-linked immunosorbent assay was initially performed to measure levels of proinflammatory mediators [nitric oxide (NO), prostaglandin E2 , and reactive oxygen species (ROS)] and cytokines [tumor necrosis factor-alpha (TNF-α), IL-1β, and IL-6], followed by reverse transcription-polymerase chain reaction and western blotting to determine mRNA and protein expression, respectively...
February 14, 2023: ACS Omega