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https://www.readbyqxmd.com/read/29846494/endothelial-differentiated-adipose-derived-stem-cells-improvement-of-survival-and-neovascularization-in-fat-transplantation
#1
William M Harris, Michael Plastini, Nikolas Kappy, Telisha Ortiz, Shaohua Chang, Spencer Brown, Jeffrey P Carpenter, Ping Zhang
Background: Adipose-derived stem cells (ASCs) assisted lipotransfer have been considered to facilitate the survival of fat grafts. However, emerging evidence of insufficient vascularization is another obstacle for fat graft survival in cell-assisted lipotransfer. Objectives: This study evaluated whether supplementation of differentiated toward an endothelial phenotype ASCs with fat lipoaspirate improves survival and neovascularization in fat transplantation. Methods: ASCs were isolated from human periumbilical fat tissue and cultured in Endothelial Growth Medium for 2wk...
May 28, 2018: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29794702/supportive-use-of-adipose-derived-stem-cells-in-cell-assisted-lipotransfer-for-localized-scleroderma
#2
Bo Chen, Xiaojun Wang, Xiao Long, Mingzi Zhang, Jiuzuo Huang, Nanze Yu, Jing Xu
BACKGROUND: The authors aimed to analyze factors related to lipotransfer for localized scleroderma, and to explore the feasibility of cell-assisted lipotransfer for localized scleroderma treatment. METHODS: Abdominal fat samples were taken from six scleroderma patients without corticosteroid therapy, five scleroderma patients with corticosteroid therapy, and 10 normal liposuction patients. Their quantity, morphology, and proliferation ability were measured. Blood flow was measured by laser speckle contrast imaging in localized scleroderma lesions and normal contralateral regions for eight localized scleroderma patients...
June 2018: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29464176/latissimus-dorsi-and-immediate-fat-transfer-lift-for-complete-autologous-breast-reconstruction
#3
James M Economides, David H Song
Background: Despite the popularity of latissimus dorsi (LD) flap in breast reconstruction, a breast implant is often necessary to achieve sufficient volume. Prior reports describe fat grafting to the LD flap as a secondary procedure to correct contour deformities and improve volume. Our institution has instituted autologous breast reconstruction with an LD flap and immediate fat transfer (LIFT). Methods: A retrospective review of all patients undergoing the LIFT procedure was undertaken...
January 2018: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/29438465/does-stromal-vascular-fraction-ensure-a-higher-survival-in-autologous-fat-grafting-for-breast-augmentation-a-volumetric-study-using-3-dimensional-laser-scanning
#4
Cheng-Hung Chiu
Background: Cell-assisted lipotransfer (CAL) has been considered a promising technique for promoting adipogenesis and angiogenesis in fat grafts. Objectives: The author sought to objectively analyze the change of breast volume in patients who underwent stromal vascular fraction (SVF)-enriched fat grafting for breast augmentation and compared the clinical results with those who underwent conventional fat grafting without SVF by using 3D laser scanning. Methods: From April 2015 to March 2016, 105 patients who underwent traditional fat grafting without SVF enrichment for breast augmentation were assigned to group A and served as the control...
February 9, 2018: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29228092/does-stromal-vascular-fraction-supplementation-improve-facial-lipotransfer
#5
Eric Swanson
No abstract text is available yet for this article.
February 17, 2018: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29228084/response-to-does-stromal-vascular-fraction-supplementation-improve-facial-lipotransfer
#6
Natale Ferreira Gontijo-de-Amorim, Luiz Charles-de-Sá, Gino Rigotti
No abstract text is available yet for this article.
February 17, 2018: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29188676/autologous-lipotransfer-versus-stromal-vascular-fraction-enriched-lipoinjection-for-diabetic-foot-wounds-healing-a-pilot-study
#7
G Vicenti, G Solarino, V Pesce, L Moretti, A Notarnicola, M Carrozzo, F Rifino, B Moretti
Chronic ulcers of the lower limbs represent a significant social and economic burden. Diabetes is a strong risk factor for development of chronic lesions. Adult stem cells and growth factors derived from the adipose tissue are among the most promising therapeutic strategies for hard to heal wounds. Fat grafts have been used for several decades to treat soft tissue deformities, but despite its excellent characteristics, the outcome was unpredictable, due to partial necrosis and resorption of the graft. Stem cells’ enrichment of these grafts or their injection into the edges of the ulcers have shown encouraging results in various experimental settings...
October 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/29175136/the-liquid-facelift-first-hands-on-experience-with-facial-water-jet-assisted-liposuction-as-an-additive-technique-for-rhytidectomy-a-case-series-of-25-patients
#8
Kai O Kaye, Sonja Kästner, Felix J Paprottka, Phillipp Gonser
OBJECTIVE: Recently, water jet-assisted liposuction (WAL) was successfully applied by several other authors to remove fat and induce skin contraction in nonfacial body areas. Extending the range of indications for this new method, the authors of this article report on their first experience with its use in facial contouring, fat harvesting, and hydrodissection of the facial skin flap in rhytidectomy in a case series of 25 patients. METHODS: Twenty-five patients (median age: 56 years) had facelift surgery under sedation, and the WAL technique was used for facial contouring, fat harvesting for facial lipotransfer, and hydrodissection of the facial skin flap...
February 2018: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/29140567/human-adipose-derived-stromal-cell-isolation-methods-and-use-in-osteogenic-and-adipogenic-in-vivo-applications
#9
Elizabeth Brett, Ruth Tevlin, Adrian McArdle, Eun Young Seo, Charles K F Chan, Derrick C Wan, Michael T Longaker
Adipose tissue represents an abundant and easily accessible source of multipotent cells, which may serve as excellent building blocks for tissue engineering. This article presents a newly described protocol for isolating adipose-derived stromal cells (ASCs) from human lipoaspirate, compared to the standard protocol for harvesting ASCs established in 2001. Human ASC isolation is performed using two methods, and resultant cells are compared through cell yield, cell viability, cell proliferation and regenerative potential...
November 15, 2017: Current Protocols in Stem Cell Biology
https://www.readbyqxmd.com/read/29052508/assessment-of-the-clinical-efficacy-of-cell-assisted-lipotransfer-and-conventional-fat-graft-a-meta-analysis-based-on-case-control-studies
#10
Yu Wang, Yanfei Wu
BACKGROUND: Cell-assisted lipotransfer is a novel technique for fat grafting. This study aimed to investigate the clinical efficacy of cell-assisted lipotransfer technology compared with conventional fat grafting. METHODS: According to PRISMA guidelines, related articles in PubMed, Embase and Cochrane library were systematically searched. Studies focusing on fat survival rate and/or patient satisfaction rate for fat grafting alone versus cell-assisted lipotransfer were retrieved...
October 19, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/29036025/the-fate-of-the-adipose-derived-stromal-cells-during-angiogenesis-and-adipogenesis-after-cell-assisted-lipotransfer
#11
Ki Yong Hong, Sangjun Yim, Hyun Jung Kim, Ung Sik Jin, SooA Lim, SuRak Eo, Hak Chang, Kyung Won Minn
BACKGROUND: Cell-assisted lipotransfer is a process in which fat grafting is supplemented with autologous adipose-derived stromal cells. Since the efficacy of the technique was demonstrated, studies have focused on the mechanism by which cell-assisted lipotransfer enhances the rate of graft survival. However, the microenvironmental changes in donor and recipient tissue associated with cell-assisted lipotransfer remain unclear. METHODS: The authors introduced an animal model of cell-assisted lipotransfer using two different transgenic reporter mice...
February 2018: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29025230/commentary-on-mechanical-supplementation-with-the-stromal-vascular-fraction-yields-improved-volume-retention-in-facial-lipotransfer-a-1-year-comparative-study
#12
Norbert Pallua, Bong-Sung Kim
No abstract text is available yet for this article.
October 1, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29025229/mechanical-supplementation-with-the-stromal-vascular-fraction-yields-improved-volume-retention-in-facial-lipotransfer-a-1-year-comparative-study
#13
Natale Ferreira Gontijo-de-Amorim, Luiz Charles-de-Sá, Gino Rigotti
Background: Unpredictable volume maintenance in the long term is a major limitation of autologous fat grafting. Objectives: The authors compared results of autologous lipotransfer to the face with or without enrichment of fat with the stromal vascular fraction (SVF). Methods: Thirty patients with asymmetric depletion of facial volume were included in a prospective study. Patients were randomly assigned to undergo a single session of autologous fat transfer with washed adipose tissue (control group) or with washed adipose tissue combined with the pellet of centrifuged lipoaspirate, which contained the SVF (enriched group)...
October 1, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29025217/use-of-lipotransfer-in-scleroderma
#14
Michelle F Griffin, Aurora Almadori, Peter E Butler
Lipotransfer for soft tissue filling is a well-established plastic and aesthetic surgical technique. Elective fat grafting is considered a safe and well-tolerated procedure. Coleman and others have reported that fat grafting may have tissue regenerative properties and not only serve as a soft tissue filler. There have been reports from our group and others that it may improve fibrosis secondary to many different pathological aetiologies including scleroderma, burn injury, lichen sclerosis, graft vs host disease, and radiation...
July 1, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/28962175/application-of-adipose-derived-stromal-cells-in-fat-grafting-basic-science-and-literature-review
#15
Margarita Moustaki, Othon Papadopoulos, Christos Verikokos, Dimitrios Karypidis, Dhalia Masud, Alkiviadis Kostakis, Florentia Papastefanaki, Maria G Roubelakis, Despoina Perrea
Autologous fat is considered the ideal material for soft-tissue augmentation in plastic and reconstructive surgery. The primary drawback of autologous fat grafting is the high resorption rate. The isolation of mesenchymal stem cells from adipose tissue inevitably led to research focusing on the study of combined transplantation of autologous fat and adipose derived stem cells (ADSCs) and introduced the theory of 'cell-assisted lipotransfer'. Transplantation of ADSCs is a promising strategy, due to the high proliferative capacity of stem cells, their potential to induce paracrine signalling and ability to differentiate into adipocytes and vascular cells...
September 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28719946/cell-assisted-lipotransfer-friend-or-foe-in-fat-grafting-systematic-review-and-meta-analysis
#16
REVIEW
J Laloze, A Varin, J Gilhodes, N Bertheuil, J L Grolleau, J Brie, J Usseglio, L Sensebe, T Filleron, B Chaput
Autologous fat grafting is a common procedure for soft-tissue reconstruction but is associated with a graft resorption rate ranging from 20% to 80%. To improve the fat graft survival rate, a new technique, called cell-assisted lipotransfer (CAL), was developed. With CAL, fat is injected along with adipose-derived stromal cells that are assumed to improve fat survival rate. We conducted an evidence-based meta-analysis to evaluate the efficacy and safety of CAL as compared with conventional autologous fat grafting (non-CAL)...
February 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28643006/complications-of-fat-transfer-for-breast-augmentation
#17
Paraskevas Kontoes, George Gounnaris
Autologous fat grafting is quite common for breast augmentations as well as for reconstructive breast surgery. Coleman has described the surgical technique of fat grafting. Fat is harvested, and after centrifugation and refinement, blunt infiltration cannulas are used to place the fat through small incisions. The grafted tissue is placed in small aliquots with each withdrawal of the cannula. In order to achieve an aesthetically pleasing contour of the breast, the fat should be layered into different levels from the chest wall to the skin...
October 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28574947/dynamic-rheology-for-the-prediction-of-surgical-outcomes-in-autologous-fat-grafting
#18
Anna Luan, Elizabeth R Zielins, Taylor Wearda, David A Atashroo, Charles P Blackshear, Jordan Raphel, Elizabeth A Brett, John Flacco, Michael C Alyono, Arash Momeni, Sarah Heilshorn, Michael T Longaker, Derrick C Wan
BACKGROUND: Because of the abundance and biocompatibility of fat, lipotransfer has become an attractive method for treating soft-tissue deficits. However, it is limited by unpredictable graft survival and retention. Currently, little is known about the viscoelastic properties of fat after various injection methods. Here, the authors assess the effects of cannula diameter, length, and shape on the viscoelastic properties, structure, and retention of fat. METHODS: Human lipoaspirate was harvested using suction-assisted liposuction and prepared for grafting...
September 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28573148/a-promising-tool-for-surgical-lipotransfer-a-constant-pressure-and-quantity-injection-device-in-facial-fat-grafting
#19
Mei Song, Yi Liu, Ping Liu, Xianying Zhang
BACKGROUND: The purpose of this study was to preliminarily assess a constant pressure and quantity fat granule injection device for minimal invasive properties in clinic. METHODS: A retrospective controlled study was carried out, from October 2013 to January 2015, on 76 female healthy patients aged between 26 and 53 years at the General Hospital of Lanzhou Military Command, China. To achieve small volume, high thrust, and precision requirements of facial fat grafting, an integrated handheld controller and motor design was adopted, reducing the volume and weight of the fat transplantation injection device...
2017: Burns and Trauma
https://www.readbyqxmd.com/read/28545495/the-impact-of-human-adipose-tissue-derived-stem-cells-on-breast-cancer-cells-implications-for-cell-assisted-lipotransfers-in-breast-reconstruction
#20
Eva Koellensperger, Lilly-Claire Bonnert, Inka Zoernig, Frederik Marmé, Stefanie Sandmann, Günter Germann, Felix Gramley, Uwe Leimer
BACKGROUND: In this study we evaluated the interactions of human adipose tissue-derived stem cells (ADSCs) and different human breast cancer cell lines (BRCAs) with regard to the safety of cell-assisted lipotransfers for breast reconstruction and a thereby unintended co-localization of ADSCs and BRCAs. METHODS: ADSCs were co-cultured with five different human BRCAs (MCF-7, MDA-MB-231, SK-BR-3, ZR-75-30, and EVSA-T) and primary BRCAs from one patient in a transwell system, and cell-cell-interactions were analyzed by assessing doubling time, migration and invasion, angiogenesis, quantitative real-time polymerase chain reaction (PCR) of more than 300 tumor-associated genes, and multiplex protein assays of 20 chemokines and growth factors and eight matrix metalloproteinases (MMPs)...
May 25, 2017: Stem Cell Research & Therapy
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