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Zeeshaan Arshad, Lindsey Karmen, Rajan Choudhary, James A Smith, Olivier A Branford, David A Brindley, David Pettitt, Benjamin M Davies
BACKGROUND: Cell assisted lipotransfer serves as a novel technique for both breast reconstruction and breast augmentation. This systematic review assesses the efficacy, safety and use of patient reported outcome measures in studies involving cell assisted lipotransfer. We also carry out an objective assessment of study quality focussing on recruitment, follow-up and provide an up-to-date clinical trial landscaping analysis. METHODS: Key electronic databases were searched according to PRISMA guidelines and pre-defined inclusion and exclusion criteria...
December 2016: JPRAS Open
Vincent Hivernaud, Bruno Lefourn, Myriam Robard, Jérôme Guicheux, Pierre Weiss
BACKGROUND: Autologous fat grafting is a widely used technique that gives natural results when treating soft tissue deficiencies. However, there is no consensus on which is the best procedure to use, leading to unpredictable results because of fat graft resorption. OBJECTIVES: This study compared four commercial lipotransfer devices by analyzing the behavior of the processed adipose tissue and outcome of the adipose graft in an in vivo model. METHODS: Four different protocols that used manual, power-assisted or water-assisted lipoaspiration and then decantation, centrifugation, or filtration were used on each of eight patients to process lipoaspirate...
February 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
Shaun S Tan, Weiliang Loh
Autologous free-fat grafting (AFG) has emerged as an attractive proposition for soft-tissue reconstruction of various contour defects because it obviates more complex reconstructive options and reduces operative times and donor-site morbidity. Nonetheless, a common complication of this procedure is the resorption of the engrafted fat. Cell-assisted lipotransfer (CAL) is now a well-regarded technique where adipose-derived stem cell (ASC)-rich stromal vascular fraction is admixed with lipoaspirate, increasing the volumetric outcome of fat grafts in light of its potent angiogenic and adipogenic properties...
November 1, 2016: Surgeon: Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland
Shaun S Tan, Zhi Yang Ng, Weiqing Zhan, Warren Rozen
Autologous fat grafting is commonly utilised to reconstruct soft tissue defects caused by ageing, trauma, chronic wounds and cancer resection. The benefits of fat grafting are minimal donor site morbidity and ease of availability through liposuction or lipectomy. Nonetheless, survival and longevity of fat grafts remain poor post-engraftment. Various methods to enhance fat graft survival are currently under investigation and its stem cell constituents are of particular interest. Cell-assisted lipotransfer refers to the addition of adipose-derived stem cell (ASC) rich component of stromal vascular fraction to lipoaspirate, the results of which have proven promising...
July 2016: Journal of Cutaneous and Aesthetic Surgery
Tommaso Agostini, Giuseppe Spinelli, Raffella Perello, Daniele Bani, Giulia Boccalini
BACKGROUND: The success of lipotransfer strongly depends on the harvesting, processing, and placement of the lipoaspirated samples. This study was designed to assess the histomorphometric characteristics and viability of fat harvested using different techniques (wet and dry) following centrifugation, as described by Coleman. METHODS: The study enrolled 85 consecutive, nonrandomized, healthy patients from March 2010 to December 2014 (45 males and 40 females). The mean age was 40 years (range, 18-59 years), and the mean body mass index was 25...
September 2016: Journal of Craniofacial Surgery
T Faltus
The use of autologous fat, especially for (stem) cell-assisted lipotransfer in plastic and aesthetic surgery, has regularly been regarded as the manufacture and application of so called Advanced Therapy Medicinal Products (ATMP). However, the in-house production of such pharmaceuticals at the point-of-care (PoC) in the surgeon's practice is not permitted without an official manufacturing license. Therefore, before beginning such treatments, a pharmaceutical manufacturing license has to be granted to the surgeon to avoid criminal prosecution and negative consequences due to infringement of professional regulations...
August 2016: Handchirurgie, Mikrochirurgie, Plastische Chirurgie
Shaun S Tan, Xiaolian Han, Priyadharshini Sivakumaran, Shiang Y Lim, Wayne A Morrison
BACKGROUND: Adipose-derived stem cells (ASCs) have applications in regenerative medicine based on their therapeutic potential to repair and regenerate diseased and damaged tissue. They are commonly subject to oxidative stress during harvest and transplantation, which has detrimental effects on their subsequent viability. By functioning as an antioxidant against free radicals, melatonin may exert cytoprotective effects on ASCs. METHODS: We cultured human ASCs in the presence of varying dosages of hydrogen peroxide and/or melatonin for a period of 3 hours...
May 2016: Archives of Plastic Surgery
Moti Harats, Eran Millet, Marie Jaeger, Arie Orenstein, Josef Haik, Steven D Hajdu, Gal Markel, Eyal Winkler, Ariel Tessone
BACKGROUND: Suction-assisted lipoplasty (SAL; liposuction) is an established aesthetic procedure in plastic surgery. The main parameters differentiating one method of lipoplasty from another are safety, consistency of results, and other more technical parameters. Due to the recent popularity of lipotransfer, the quality of extracted fat has become a relevant parameter. We compare the viability of extracted adipocytes after dry SAL, hyper-tumescent PAL (power-assisted lipoplasty), and water-assisted lipoplasty (WAL)...
August 2016: Aesthetic Plastic Surgery
R Kumar, M Griffin, G Adigbli, N Kalavrezos, P E M Butler
BACKGROUND: Radiation-induced fibrosis (RIF) is a late complication of radiotherapy that results in progressive functional and cosmetic impairment. Autologous fat has emerged as an option for soft tissue reconstruction. There are also sporadic reports suggesting regression of fibrosis following regional lipotransfer. This systematic review aimed to identify cellular mechanisms driving RIF, and the potential role of lipotransfer in attenuating these processes. METHODS: PubMed, OVID and Google Scholar databases were searched to identify all original articles regarding lipotransfer for RIF...
July 2016: British Journal of Surgery
Navid Mohamadpour Toyserkani, Charlotte Harken Jensen, Søren Paludan Sheikh, Jens Ahm Sørensen
UNLABELLED: : Lymphedema is one of the most frequent side effects following cancer treatment, and treatment opportunities for it are currently lacking. Stem cell therapy has been proposed as a possible novel treatment modality. This study was the first case in which freshly isolated adipose-derived stromal cells were used to treat lymphedema. Treatment was given as a cell-assisted lipotransfer in which 4.07 × 10(7) cells were injected with 10 ml of lipoaspirate in the axillary region...
July 2016: Stem Cells Translational Medicine
Vipra Guneta, Nguan Soon Tan, Shigeki Sugii, Thiam Chye Lim, Thien Chong Marcus Wong, Cleo Choong
BACKGROUND: Abdominal tissue enriched with adipose-derived stem cells (ASCs) is often used in cell-assisted lipotransfer procedures for breast reconstruction. However, as the tissue microenvironment and stem cell niche play important roles in defining the characteristics of the resident cells, it is hypothesized that the stem cell population present in the donor abdominal tissue has dissimilar properties as compared with the cells in the recipient breast tissue, which may ultimately affect the long-term success of the graft...
May 2016: Annals of Plastic Surgery
Wenshan Xing, Dali Mu, Qian Wang, Su Fu, Minqiang Xin, Jie Luan
BACKGROUND: Stem cells have proven to be beneficial to fat graft survival, but a one-step method of cell-assisted lipotransfer is still missing. In the present work, the authors improved the fat graft survival using bone marrow aspirate and bone marrow concentrate, to ensure that both liposuction and cell-assisted lipotransfer were included in the same procedure. METHODS: Bone marrow aspirate was collected from the iliac crest of the rabbits. Bone marrow concentrate was obtained using density gradient centrifugation and labeled with PKH26 fluorescent cell linker...
April 2016: Plastic and Reconstructive Surgery
Elizabeth R Zielins, Elizabeth A Brett, Michael T Longaker, Derrick C Wan
An invaluable part of the plastic surgeon's technical arsenal for soft tissue contouring, fat grafting continues to be plagued by unpredictable outcomes, resulting in either reoperation and/or patient dissatisfaction. Thus, extensive research has been conducted into the effects of adipose tissue procurement, processing, and placement on fat graft quality at both the cellular level and in terms of overall volume retention. Herein, we present an overview of the vast body of literature in these areas, with additional discussion of cell-assisted lipotransfer as a therapy to improve volume retention, and on the controversial use of autologous fat in the setting of prior irradiation...
April 2016: Aesthetic Surgery Journal
Sammy Sinno, Stelios Wilson, Nicholas Brownstone, Steven M Levine
BACKGROUND: Autologous fat grafting is an increasingly popular procedure used for facial rejuvenation and body contouring. The purpose of this article is to perform an evidence-based review to determine fact from fiction for the hot topics in autologous fat grafting. METHODS: A comprehensive literature search was performed. The following key words were then searched: "fat grafting," "autologous fat grafting," "autologous fat transfer," "lipotransfer," "liposculping," and "lipofilling...
March 2016: Plastic and Reconstructive Surgery
Navid Mohamadpour Toyserkani, Marlene Louise Quaade, Jens Ahm Sørensen
INTRODUCTION: Autologous lipotransfer is seen as an ideal filler for soft tissue reconstruction. The main limitation of this procedure is the unpredictable resorption and volume loss of the fat graft. In the recent decade, an increasing amount of research has focused on the use of adipose tissue-derived stromal cells (ASCs) to enrich the fat graft, a procedure termed cell-assisted lipotransfer (CAL). The aim of this review was to systematically review the current preclinical and clinical evidence for the efficacy of CAL compared with conventional lipotransfer...
April 2016: Aesthetic Plastic Surgery
Qianwen Wang, Xiao Wang, Lu Zhou, Jiaqi Wang, Tailing Wang
BACKGROUND: Many studies have shown that autologous fat grafting and cell-assisted lipotransfer can improve skin quality, with both mature adipocytes (MAs) and adipose-derived stem cells (ADSCs) considered to play a role. However, it is unclear whether transplantation of ADSCs or MAs has the same impact on the overlying skin. METHODS: Nine pigs were divided into 3 equal groups, with lipoaspirates obtained from the porcine dorsum of each pig. Transplant content was injected into the right upper eyelids, while the left eyelids were used as controls...
January 2017: Ophthalmic Plastic and Reconstructive Surgery
Yiwen Zhou, Jing Wang, Haizhou Li, Xiao Liang, Jinhong Bae, Xiaolu Huang, Qingfeng Li
BACKGROUND: The main drawback of autologous fat grafting, which is commonly used for soft-tissue augmentation, is the high resorption rate. Cell-assisted lipotransfer has been used to improve fat graft survival; however, evidence for its efficacy and safety is still lacking. METHODS: The authors searched PubMed, Cochrane Library, EBSCO, and EMBASE for clinical studies on cell-assisted lipotransfer published from 2008 through 2014. A meta-analysis was conducted to pool the estimated fat survival rate...
January 2016: Plastic and Reconstructive Surgery
Rafi Fredman, Renee E Edkins, Charles Scott Hultman
BACKGROUND: Chronic neuropathic pain after burn injury is a significant problem that affects up to 29% of burn patients. Neuropathic burn scar pain is a challenge for plastic and burn surgeons, who have limited solutions. Fat grafting, with its mechanical and regenerative qualities, can improve neuropathic pain from various traumatic and postsurgical etiologies, but its effectiveness in neuropathic burn scar pain has yet to be demonstrated. In this study, the possible role of lipotransfer in treating neuropathic burn scar pain is explored, focusing on safety, graft take, and short-term efficacy...
June 2016: Annals of Plastic Surgery
Sheng Huang, Weiliang Zhao, Zihua Wang, Kai Tao, Xiaoyan Liu, Peng Chang
Cell-assisted lipotransfer (CAL) has been widely used in various clinical applications, including breast augmentation following mammectomy, soft-tissue reconstruction and wound healing. However, the clinical application of CAL has been restricted due to the transplanted fat tissues being readily liquefied and absorbed. The present review examines 57 previously published studies involving CAL, including fat grafting or fat transfer with human adipose-stem cells in all known databases. Of these 57 articles, seven reported the clinical application of CAL...
February 2016: Molecular Medicine Reports
Jun Zhang, Xiaozhi Bai, Bin Zhao, Yunchuan Wang, Linlin Su, Peng Chang, Xujie Wang, Shichao Han, Jianxin Gao, Xiaolong Hu, Dahai Hu, Xiaoyan Liu
Autologous adipose-derived stem cells (ADSCs) can protect fat grafts in cell-assisted lipotransfer (CAL). However, diabetes alters the intrinsic properties of ADSCs and impairs their function so that they lack these protective effects. We investigate whether allogeneic ADSCs from healthy donors could protect fat grafts in immunocompetent diabetic rats. Syngeniec adipose tissues and ADSCs were derived from diabetic Lewis (LEW) rats, whereas allogeneic ADSCs were from healthy brown-Norway rats. A grafted mixture containing 0...
May 2016: Cell and Tissue Research
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