keyword
MENU ▼
Read by QxMD icon Read
search

Thioredoxin reductase

keyword
https://www.readbyqxmd.com/read/28702378/thioredoxin-a-is-essential-for-motility-and-contributes-to-host-infection-of-listeria-monocytogenes-via-redox-interactions
#1
Changyong Cheng, Zhimei Dong, Xiao Han, Hang Wang, Li Jiang, Jing Sun, Yongchun Yang, Tiantian Ma, Chunyan Shao, Xiaodu Wang, Zhongwei Chen, Weihuan Fang, Nancy E Freitag, Huarong Huang, Houhui Song
Microbes employ the thioredoxin system to defend against oxidative stress and ensure correct disulfide bonding to maintain protein function. Listeria monocytogenes has been shown to encode a putative thioredoxin, TrxA, but its biological roles and underlying mechanisms remain unknown. Here, we showed that expression of L. monocytogenes TrxA is significantly induced in bacteria treated with the thiol-specific oxidizing agent, diamide. Deletion of trxA markedly compromised tolerance of the pathogen to diamide, and mainly impaired early stages of infection in human intestinal epithelial Caco-2 cells...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28702279/the-utilization-of-selenocysteine-trna-ser-sec-isoforms-is-regulated-in-part-at-the-level-of-translation-in-vitro
#2
Bradley A Carlson, Nirupama Gupta, Mark H Pinkerton, Dolph L Hatfield, Paul R Copeland
The tRNA for the 21st proteinogenic amino acid, selenocysteine, exists in mammalian cells as 2 isoforms differing by a single 2'-O-methylribosyl moiety at position 34 (Um34). These isoforms contain either 5-methoxycarbonylmethyluridine (mcm(5)U) or 5-methoxycarbonylmethyl-2'-O-methyluridine (mcm(5)Um) at position 34. The accumulation of the mcm(5)Um isoform is tightly correlated with the expression of nonessential "stress response" selenoproteins such as glutathione peroxidase 1 (GPX1). The expression of essential selenoproteins, such as thioredoxin reductase 1 (TXNRD1), is not affected by changes in Sec-tRNA([Ser]Sec) isoform accumulation...
2017: Translation
https://www.readbyqxmd.com/read/28698569/ahpc-of-the-mycobacterial-antioxidant-defense-system-and-its-interaction-with-its-reducing-partner-thioredoxin-c
#3
Chui Fann Wong, Joon Shin, Malathy Sony Subramanian Manimekalai, Wuan Geok Saw, Zhan Yin, Shashi Bhushan, Arvind Kumar, Priya Ragunathan, Gerhard Grüber
Despite the highly oxidative environment of the phagosomal lumen, the need for maintaining redox homeostasis is a critical aspect of mycobacterial biology. The pathogens are equipped with the sophisticated thioredoxin- (Trx) and peroxiredoxin system, including TrxC and the alkyl hydroperoxide reductase subunit C (AhpC), whereby TrxC is one of the reducing partners of AhpC. Here we visualize the redox modulated dodecamer ring formation of AhpC from Mycobacterium bovis (BCG strain; MbAhpC) using electron microscopy and present novel insights into the unique N-terminal epitope (40 residues) of mycobacterial AhpC...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28692378/photosynthetic-activity-of-cotyledons-is-critical-during-post-germinative-growth-and-seedling-establishment
#4
Valle Ojeda, Victoria A Nájera, Maricruz González, Juan M Pérez-Ruiz, Francisco J Cejudo
Thioredoxins (Trxs) play a relevant role in thiol-dependent redox regulation, which allows the rapid adaptation of chloroplast metabolism to unpredictable environmental conditions. In chloroplasts, Trxs use reducing equivalents provided by photoreduced ferredoxin (Fdx) via the action of a ferredoxin-thioredoxin reductase (FTR), thus linking redox regulation to light. In addition, these organelles contain an NADPH-thioredoxin reductase, NTRC, with a Trx domain at the C-terminus. NTRC efficiently reduces 2-Cys peroxiredoxins (Prxs), hence having antioxidant function...
July 10, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28688915/targeted-proteome-analysis-with-isotope-coded-protein-labels-for-monitoring-the-influence-of-dietary-phytochemicals-on-the-expression-of-cytoprotective-proteins-in-primary-human-colon-cells
#5
Christiane Östreicher, Sven Bartenbacher, Monika Pischetsrieder
Cytoprotective effects by upregulating cellular expression levels of antioxidant proteins are attributed to a significant number of food ingredients. Evaluation of those cytoprotective effects and identification of the most active components requires reliable and comprehensive proteomic strategies. Thus, promising potential bioactive ingredients could be determined for the prevention of various diseases, including colon cancer formation. We established a multiplexed nanoLC-MS/MS targeted proteomic method, operated in scheduled selected reaction monitoring mode (sSRM), to record modulation of the expression levels of six major antioxidant proteins induced by dietary phytochemicals...
July 6, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28687608/thioredoxin-reductase-from-toxoplasma-gondii-an-essential-virulence-effector-with-antioxidant-function
#6
Junxin Xue, Wei Jiang, Yongjun Chen, Fengju Gong, Minyan Wang, Peng Zeng, Can Xia, Quan Wang, Kehe Huang
Thioredoxin reductase (TR) can help pathogens resist oxidative-burst injury from host immune cells by maintaining a thioredoxin-reduction state during NADPH consumption. TR is a necessary virulence factor that enables the persistent infection of some parasites. We performed bioinformatics analyses and biochemical assays to characterize the activity, subcellular localization, and genetic ablation of Toxoplasma gondii TR (TgTR), to shed light on its biologic function. We expressed the TgTR protein with an Escherichia coli expression system and analyzed its enzyme activity, reporting a Km for the recombinant TgTR of 11...
July 7, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28686716/gsr-is-not-essential-for-the-maintenance-of-antioxidant-defenses-in-mouse-cochlea-possible-role-of-the-thioredoxin-system-as-a-functional-backup-for-gsr
#7
Chul Han, Mi-Jung Kim, Dalian Ding, Hyo-Jin Park, Karessa White, Logan Walker, Tongjun Gu, Masaru Tanokura, Tatsuya Yamasoba, Paul Linser, Richard Salvi, Shinichi Someya
Glutathione reductase (GSR), a key member of the glutathione antioxidant defense system, converts oxidized glutathione (GSSG) to reduced glutathione (GSH) and maintains the intracellular glutathione redox state to protect the cells from oxidative damage. Previous reports have shown that Gsr deficiency results in defects in host defense against bacterial infection, while diquat induces renal injury in Gsr hypomorphic mice. In flies, overexpression of GSR extended lifespan under hyperoxia. In the current study, we investigated the roles of GSR in cochlear antioxidant defense using Gsr homozygous knockout mice that were backcrossed onto the CBA/CaJ mouse strain, a normal-hearing strain that does not carry a specific Cdh23 mutation that causes progressive hair cell degeneration and early onset of hearing loss...
2017: PloS One
https://www.readbyqxmd.com/read/28684416/thioredoxin-reductase-1-and-nadph-directly-protect-protein-tyrosine-phosphatase-1b-from-inactivation-during-h2o2-exposure
#8
Markus Dagnell, Paul E Pace, Qing Cheng, Jeroen Frijhoff, Arne Östman, Elias S J Arnér, Mark B Hampton, Christine C Winterbourn
Regulation of growth factor signaling involves reversible inactivation of protein tyrosine phosphatases (PTPs) through the oxidation and reduction of their active site cysteine. However, there is limited mechanistic understanding of these redox events and their coordination in the presence of cellular antioxidant networks. Here, we investigated interactions between PTP1B and the peroxiredoxin 2 (Prx2)/thioredoxin 1 (Trx1)/thioredoxin reductase 1 (TrxR1) network. We found that Prx2 becomes oxidized in PDGF-treated fibroblasts, but only when TrxR1 has first been inhibited...
July 6, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28675847/mononuclear-gold-iii-complexes-with-phenanthroline-ligands-as-efficient-inhibitors-of-angiogenesis-a-comparative-study-with-auranofin-and-sunitinib
#9
Aleksandar Pavic, Biljana Đ Glišić, Sandra Vojnovic, Beata Warżajtis, Nada D Savić, Marija Antić, Slavko Radenković, Goran V Janjić, Jasmina Nikodinovic-Runic, Urszula Rychlewska, Miloš I Djuran
Gold(III) complexes with 1,7- and 4,7-phenanthroline ligands, [AuCl3(1,7-phen-κN7)] (1) and [AuCl3(4,7-phen-κN4)] (2) were synthesized and structurally characterized by spectroscopic (NMR, IR and UV-vis) and single-crystal X-ray diffraction techniques. In these complexes, 1,7- and 4,7-phenanthrolines are monodentatedly coordinated to the Au(III) ion through the N7 and N4 nitrogen atoms, respectively. In comparison to the clinically relevant anti-angiogenic compounds auranofin and sunitinib, gold(III)-phenanthroline complexes showed from 1...
September 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28662462/alterations-of-growth-antioxidant-system-and-gene-expression-in-stylosanthes-guianensis-during-colletotrichum-gloeosporioides-infection
#10
Hui Wang, Zhijian Chen, Guodao Liu, Changjun Bai, Hong Qiu, Yanxing Jia, Lijuan Luo
Anthracnose caused by Colletotrichum gloeosporioides is one of the most destructive fungal diseases of many plants, including stylo (Stylosanthes spp.), which is an important tropical forage legume. Although C. gloeosporioides-caused anthracnose is the major constraint limiting the growth and yield of stylo, little information is available regarding the responses of stylo during the infection process of this pathogen. This study investigated the changes in growth, the antioxidant system and gene expression in stylo in response to C...
June 20, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28660949/selenoproteins-are-involved-in-antioxidant-defense-systems-in-thalassemia
#11
REVIEW
G E Genc, Z Ozturk, S Gumuslu
Thalassemia major (TM) is a hereditary blood disease that affects the production of hemoglobin, resulting in severe anemia. Iron overload because of repeated blood transfusion and increased intestinal iron absorption and hemolysis are the major causes of increased oxidative stress in these patients. Growth and maturational delay, cardiomyopathy, endocrinopathies, and osteoporosis are the complications of thalassemia, secondary to anemia and iron overload. The human body has endogenous defense mechanisms to help protect against free radical-induced cell damage...
June 29, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28659344/guanylyl-cyclase-sensitivity-to-nitric-oxide-is-protected-by-a-thiol-oxidation-driven-interaction-with-thioredoxin1
#12
Can Huang, Maryam Alapa, Ping Shu, Narayan Nagarajan, Changgong Wu, Junichi Sadoshima, Vladyslav Kholodovych, Hong Li, Annie Beuve
Nitric oxide (NO) modulates many physiological events through production of cGMP from its receptor, the NO-sensitive guanylyl cyclase (GC1). NO also appears to function in a cGMP independent manner, via S-nitrosation (SNO), a redox-based modification of cysteine thiols. Previously we have shown that S-nitrosated GC1 (SNO-GC1) is desensitized to NO stimulation following prolonged NO exposure or under oxidative/nitrosative stress. In animal models of nitrate tolerance and angiotensin II-induced hypertension, decreased vasodilation in response to NO correlates with GC1 thiol oxidation, but the physiological mechanism that resensitizes GC1 to NO and restores basal activity is unknown...
June 28, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28658624/hepatocyte-hyperproliferation-upon-liver-specific-co-disruption-of-thioredoxin-1-thioredoxin-reductase-1-and-glutathione-reductase
#13
Justin R Prigge, Lucia Coppo, Sebastin S Martin, Fernando Ogata, Colin G Miller, Michael D Bruschwein, David J Orlicky, Colin T Shearn, Jean A Kundert, Julia Lytchier, Alix E Herr, Åse Mattsson, Matthew P Taylor, Tomas N Gustafsson, Elias S J Arnér, Arne Holmgren, Edward E Schmidt
Energetic nutrients are oxidized to sustain high intracellular NADPH/NADP(+) ratios. NADPH-dependent reduction of thioredoxin-1 (Trx1) disulfide and glutathione disulfide by thioredoxin reductase-1 (TrxR1) and glutathione reductase (Gsr), respectively, fuels antioxidant systems and deoxyribonucleotide synthesis. Mouse livers lacking both TrxR1 and Gsr sustain these essential activities using an NADPH-independent methionine-consuming pathway; however, it remains unclear how this reducing power is distributed...
June 27, 2017: Cell Reports
https://www.readbyqxmd.com/read/28653098/overexpression-of-glutathione-peroxidase-1-predicts-poor-prognosis-in-oral-squamous-cell-carcinoma
#14
Jae Ryung Lee, Jong-Lyel Roh, Sun Mi Lee, Yangsoon Park, Kyung-Ja Cho, Seung-Ho Choi, Soon Yuhl Nam, Sang Yoon Kim
PURPOSE: Intracellular antioxidant enzymes are commonly upregulated in various cancer types and are associated with treatment outcomes. Because the relationship has rarely been examined in oral squamous cell carcinoma (OSCC), we aimed to evaluate the association between the levels of glutathione peroxidase (GPX)1, GPX4, and thioredoxin reductase (TrxR)1 expression and prognosis in patients with OSCC who underwent curative surgical resection. METHODS: This study included 233 patients who underwent curative surgery for previously untreated OSCC between 2000 and 2012...
June 26, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28648527/targeting-the-thioredoxin-system-for-cancer-therapy
#15
REVIEW
Junmin Zhang, Xinming Li, Xiao Han, Ruijuan Liu, Jianguo Fang
Thioredoxin (Trx) and thioredoxin reductase (TrxR) are essential components of the Trx system which plays pivotal roles in regulating multiple cellular redox signaling pathways. In recent years TrxR/Trx have been increasingly recognized as an important modulator of tumor development, and hence targeting TrxR/Trx is a promising strategy for cancer treatment. In this review we first discuss the structural details of TrxR, the functions of the Trx system, and the rational of targeting TrxR/Trx for cancer treatment...
June 22, 2017: Trends in Pharmacological Sciences
https://www.readbyqxmd.com/read/28647579/frailty-is-associated-with-lower-expression-of-genes-involved-in-cellular-response-to-stress-results-from-the-toledo-study-for-healthy-aging
#16
Mariam El Assar, Javier Angulo, José Antonio Carnicero, Stefan Walter, Francisco José García-García, Eva López-Hernández, José-María Sánchez-Puelles, Leocadio Rodríguez-Mañas
BACKGROUND: Specific mechanisms underlying frailty syndrome are not well known. Frailty can be viewed as a loss of functional reserve resulting in increased vulnerability to stressors. We hypothesize that pathways regulating cellular response to stress are potential players in the development of frailty. The aim of this study was to evaluate the association of the expression of certain genes related to cellular response to stress with the presence of frailty in older patients. METHODS: A sample of 350 individuals aged 65 years or older (22% frail) was selected from the Toledo Study of Healthy Aging...
June 21, 2017: Journal of the American Medical Directors Association
https://www.readbyqxmd.com/read/28640807/lack-of-a-peroxiredoxin-suppresses-the-lethality-of-cells-devoid-of-electron-donors-by-channelling-electrons-to-oxidized-ribonucleotide-reductase
#17
Susanna Boronat, Alba Domènech, Mercè Carmona, Sarela García-Santamarina, M Carmen Bañó, José Ayté, Elena Hidalgo
The thioredoxin and glutaredoxin pathways are responsible of recycling several enzymes which undergo intramolecular disulfide bond formation as part of their catalytic cycles such as the peroxide scavengers peroxiredoxins or the enzyme ribonucleotide reductase (RNR). RNR, the rate-limiting enzyme of deoxyribonucleotide synthesis, is an essential enzyme relying on these electron flow cascades for recycling. RNR is tightly regulated in a cell cycle-dependent manner at different levels, but little is known about the participation of electron donors in such regulation...
June 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28636040/tamoxifen-like-metallocifens-target-the-thioredoxin-system-determining-mitochondrial-impairment-leading-to-apoptosis-in-jurkat-cells
#18
Valeria Scalcon, Michèle Salmain, Alessandra Folda, Siden Top, Pascal Pigeon, Hui Zhi Shirley Lee, Gérard Jaouen, Alberto Bindoli, Anne Vessières, Maria Pia Rigobello
Tamoxifen-like metallocifens (TLMs) of the group-8 metals (Fe, Ru, and Os) show strong anti-proliferative activity on cancer cell lines resistant to apoptosis, owing to their unique redox properties. In contrast, the thioredoxin system, which is involved in cellular redox balance, is often overexpressed in cancer cells, especially in tumour types resistant to standard chemotherapies. Therefore, we investigated the effect of these three TLMs on the thioredoxin system and evaluated the input of the metallocene unit in comparison with structurally related organic tamoxifens...
July 19, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28629723/microbial-antioxidant-defense-enzymes
#19
REVIEW
Cindy Staerck, Amandine Gastebois, Patrick Vandeputte, Alphonse Calenda, Gérald Larcher, Louiza Gillmann, Nicolas Papon, Jean-Philippe Bouchara, Maxime J J Fleury
Free radicals are often described as chemical compounds characterized by unpaired electrons in their outer orbital rendering them highly reactive species. In mammalians, studies on free radicals were focused on reactive oxygen species (ROS) or reactive nitrogen species (RNS) due to their relative importance in physiological as well as in pathological processes. These cellular compounds are produced by different physiological systems such as the aerobic metabolism and play a major role in cell signaling pathways but also in the host immune defenses against pathogenic microorganisms...
June 16, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28624622/preclinical-anti-cancer-activity-and-multiple-mechanisms-of-action-of-a-cationic-silver-complex-bearing-n-heterocyclic-carbene-ligands
#20
Simon J Allison, Maria Sadiq, Efstathia Baronou, Patricia A Cooper, Chris Dunnill, Nikolaos T Georgopoulos, Ayşe Latif, Samantha Shepherd, Steve D Shnyder, Ian J Stratford, Richard T Wheelhouse, Charlotte E Willans, Roger M Phillips
Organometallic complexes offer the prospect of targeting multiple pathways that are important in cancer biology. Here, the preclinical activity and mechanism(s) of action of a silver-bis(N-heterocyclic carbine) complex (Ag8) were evaluated. Ag8 induced DNA damage via several mechanisms including topoisomerase I/II and thioredoxin reductase inhibition and induction of reactive oxygen species. DNA damage induction was consistent with cytotoxicity observed against proliferating cells and Ag8 induced cell death by apoptosis...
June 15, 2017: Cancer Letters
keyword
keyword
8641
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"