Jeffrey J Czajka, Yichao Han, Joonhoon Kim, Stephen J Mondo, Beth A Hofstad, AnaLaura Robles, Sajeet Haridas, Robert Riley, Kurt LaButti, Jasmyn Pangilinan, William Andreopoulos, Anna Lipzen, Juying Yan, Mei Wang, Vivian Ng, Igor V Grigoriev, Joseph W Spatafora, Jon K Magnuson, Scott E Baker, Kyle R Pomraning
The Lipomyces clade contains oleaginous yeast species with advantageous metabolic features for biochemical and biofuel production. Limited knowledge about the metabolic networks of the species and limited tools for genetic engineering have led to a relatively small amount of research on the microbes. Here, a genome-scale metabolic model (GSM) of Lipomyces starkeyi NRRL Y-11557 was built using orthologous protein mappings to model yeast species. Phenotypic growth assays were used to validate the GSM (66% accuracy) and indicated that NRRL Y-11557 utilized diverse carbohydrates but had more limited catabolism of organic acids...
2024: Frontiers in Bioengineering and Biotechnology