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https://www.readbyqxmd.com/read/28641882/ferredoxin-dependent-bilin-reductases-in-eukaryotic-algae-ubiquity-and-diversity
#1
Nathan C Rockwell, J Clark Lagarias
Linear tetrapyrroles (bilins) are produced from heme by heme oxygenase, usually forming biliverdin IXα (BV). Fungi and bacteria use BV as chromophore for phytochrome photoreceptors. Oxygenic photosynthetic organisms use BV as a substrate for ferredoxin-dependent bilin reductases (FDBRs), enzymes that produce diverse reduced bilins used as light-harvesting pigments in phycobiliproteins and as photoactive photoreceptor chromophores. Bilin biosynthesis is essential for phototrophic growth in Chlamydomonas reinhardtii despite the absence of phytochromes or phycobiliproteins in this organism, raising the possibility that bilins are more generally required for phototrophic growth by algae...
May 31, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28607990/reaction-intermediates-in-the-heme-degradation-reaction-by-hutz-from-vibrio-cholerae
#2
Takeshi Uchida, Yukari Sekine, Nobuhiko Dojun, Ariel Lewis-Ballester, Izumi Ishigami, Toshitaka Matsui, Syun-Ru Yeh, Koichiro Ishimori
HutZ is a heme-degrading enzyme in Vibrio cholerae. It converts heme to biliverdin via verdoheme, suggesting that it follows the same reaction mechanism as that of mammalian heme oxygenase. However, none of the key intermediates have been identified. In this study, we applied steady-state and time-resolved UV-vis absorption and resonance Raman spectroscopy to study the reaction of the heme-HutZ complex with H2O2 or ascorbic acid. We characterized three intermediates: oxyferrous heme, meso-hydroxyheme, and verdoheme complexes...
June 13, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28602760/how-to-increase-brightness-of-near-infrared-fluorescent-proteins-in-mammalian-cells
#3
Anton A Shemetov, Olena S Oliinyk, Vladislav V Verkhusha
Numerous near-infrared (NIR) fluorescent proteins (FPs) were recently engineered from bacterial photoreceptors but lack of their systematic comparison makes researcher's choice rather difficult. Here we evaluated side-by-side several modern NIR FPs, such as blue-shifted smURFP and miRFP670, and red-shifted mIFP and miRFP703. We found that among all NIR FPs, miRFP670 had the highest fluorescence intensity in various mammalian cells. For instance, in common HeLa cells miRFP703, mIFP, and smURFP were 2-, 9-, and 53-fold dimmer than miRFP670...
June 22, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28601647/metalloproteomic-and-differential-expression-in-plasma-in-a-rat-model-of-type-1-diabetes
#4
Camila Pereira Braga, José Cavalcante Souza Vieira, Aline de Lima Leite, Ana Angélica Henrique Fernandes, Marília Afonso Rabelo Buzalaf, Pedro de Magalhães Padilha
Type 1 diabetes is characterized by hyperglycemia, which in the chronic stage is associated with abnormalities in lipids, protein and, carbohydrate metabolism, as well as oxidative stress. New strategies for prevention and treatment are needed, as type 1 diabetes affects life quality and survival, and involves high-cost treatment. Proteomic and metalloproteomic studies can elucidate the functional and physiological aspects of biomolecules. In the present study, differential proteomics was used to identify potential biomarkers of diabetes in rat plasma associated with copper, selenium, zinc, and magnesium fractionation in control and diabetic rats, as well as diabetic rats treated with insulin...
June 8, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28588733/differences-in-the-sensitivity-of-ovarian-cancer-to-photodynamic-therapy-and-the-mechanisms-for-those-differences
#5
Yoshihito Yokoyama, Tatsuhiko Shigeto, Rie Miura, Asami Kobayashi, Makito Mizunuma, Aisa Yamauchi, Masayuki Futagami, Hideki Mizunuma
Protoporphyrin IX (PpIX) levels are crucial to the antitumor action of photodynamic therapy (PDT). In the present study, the underling molecular mechanisms for the variation in PpIX levels in ovarian cancer cells were investigated. Five ovarian cancer cell lines were subcutaneously grafted onto the backs of nude mice. Once tumors had developed, 5-aminolevulinic acid methyl ester hydrochloride (methyl-ALA) was administered intraperitoneally and the tumor was irradiated twice/week. PpIX levels in the tumor were assayed using high-performance liquid chromatography...
June 2017: Oncology Letters
https://www.readbyqxmd.com/read/28576353/reprint-of-heme-oxygenase-1-affects-granulopoiesis-in-mice-through-control-of-myelocyte-proliferation
#6
Karolina Bukowska-Strakova, Maciej Ciesla, Krzysztof Szade, Witold Norbert Nowak, Robert Straka, Agata Szade, Malgorzata Tyszka-Czochara, Karolina Najder, Anna Konturek, Maciej Siedlar, Jozef Dulak, Alicja Jozkowicz
Heme oxygenase-1 (HO-1) is stress-inducible, cytoprotective enzyme degrading heme to carbon monoxide (CO), biliverdin and Fe(2+). We showed that HO-1 knock-out mice (HO-1(-/-)) have a twofold higher level of granulocytes than wild type (WT) mice, despite decreased concentration of granulocyte colony-stimulating factor (G-CSF) in the blood and reduced surface expression of G-CSF receptor on the hematopoietic precursors. This suggests the effect of HO-1 on granulopoiesis. Here we aimed to determine the stage of granulopoiesis regulated by HO-1...
June 2017: Immunobiology
https://www.readbyqxmd.com/read/28566503/heavy-metal-suicide
#7
Andreas Linkermann, Brent R Stockwell, Tom Vanden Berghe
In acute kidney injury (AKI), the majority of dying tubular cells succumbs to an iron-dependent form of regulated necrosis, referred to as ferroptosis. Ferroptosis is essentially mediated by iron-catalyzed lipid peroxidation upon GPX4 dysfunction. Heme oxygenase 1 (HO-1) is a master regulator of intracellular free iron due to the conversion of heme to iron, carbon monoxide, and biliverdin(12), and therefore represents a potential regulator of ferroptotic cell death. In this issue of AJP - Renal Physiology, Adedoyin et al...
May 31, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28560878/serial-metabolome-changes-in-a-prospective-cohort-of-subjects-with-influenza-viral-infection-and-comparison-with-dengue-fever
#8
Liang Cui, Jinling Fang, Eng Eong Ooi, Yie Hou Lee
Influenza virus infection (IVI) and dengue virus infection (DVI) are major public health threats. Between IVI and DVI, clinical symptoms can be overlapping yet infection-specific, but host metabolome changes are not well-described. Untargeted metabolomics and targeted oxylipinomic analyses were performed on sera serially collected at three phases of infection from a prospective cohort study of adult subjects with either H3N2 influenza infection or dengue fever. Untargeted metabolomics identified 26 differential metabolites and major perturbed pathways included purine metabolism, fatty acid biosynthesis and β-oxidation, tryptophan metabolism, phospholipid catabolism and steroid hormone pathway...
May 31, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28560357/impact-of-heme-on-specific-antibody-production-in-mice-promotive-inhibitive-or-null-outcome-is-determined-by-its-concentration
#9
Guofu Li, Haiyan Xue, Zeng Fan, Yun Bai
Free heme is an endogenous danger signal that provokes innate immunity. Active innate immunity provides a precondition of an effective adaptive immune response. However, heme catabolites, CO, biliverdin and bilirubin trigger immunosuppression. Furthermore, free heme induces expression of heme oxygenase-1 to increase production of CO, biliverdin and bilirubin. As such, free heme can play a paradoxical role in adaptive immunity. What is the outcome of the animal immune response to an antigen in the presence of free heme? This question remains to be explored...
May 2017: Heliyon
https://www.readbyqxmd.com/read/28554854/hgf-induces-emt-in-non-small-cell-lung-cancer-through-the-hbvr-pathway
#10
Fang Liu, Shasha Song, Zhi Yi, Min Zhang, Jiali Li, Fang Yang, Hongtao Yin, Xiufeng Yu, Chao Guan, Ying Liu, Zizhen Liu, Jing Wang, Daling Zhu
The epithelial-to-mesenchymal transition (EMT) is a crucial event during non-small-cell lung cancer (NSCLC) invasion and metastasis. However, the mechanisms involved in NSCLC EMT have not been fully clarified. Hepatocyte growth factor (HGF) and human biliverdin reductase (hBVR) are reported to contribute to EMT in several diseases. Here, we show that compared with transforming growth factor beta (TGF-β), fibroblast growth factor (FGF), and epidermal growth factor (EGF), HGF is an important cell factor for EMT in NSCLC cell lines A549 and H460...
May 26, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28500721/structural-and-vibrational-characterization-of-the-chromophore-binding-site-of-bacterial-phytochrome-agp1
#11
Aref Takiden, Francisco Velazquez-Escobar, Jovan Dragelj, Anna Lena Woelke, Ernst-Walter Knapp, Patrick Piwowarski, Franz Bart, Peter Hildebrandt, Maria Andrea Mroginski
Agp1 is a prototypical bacterial phytochrome from Agrobacterium fabrum harboring a biliverdin cofactor which reversibly photoconverts between a red-light-absorbing (Pr) and a far-red-light-absorbing (Pfr) states. The reaction mechanism involves the isomerization of the bilin-chromophore followed by large structural changes of the protein matrix that are coupled to protonation dynamics at the chromophore binding site. Histidines His250 and His280 participate in this process. Although the three-dimensional structure of Agp1 has been solved at high resolution, the precise position of hydrogen atoms and protonation pattern in the chromophore binding pocket has not been investigated yet...
May 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/28499219/oxidant-and-anti-oxidant-status-in-common-brain-tumors-correlation-to-tp53-and-human-biliverdin-reductase
#12
Pinar Atukeren, Sena Oner, Oguz Baran, Rahsan Kemerdere, Bulent Eren, Ufuk Cakatay, Taner Tanriverdi
OBJECTIVE: To assess oxidant and antioxidant status in patients with common brain tumors; namely meningiomas, low-grade gliomas (LGG) and high-grade gliomas (HGG) and to compare with normal brain tissues. PATIENTS AND METHODS: Almost nine biomarkers were measured in 59 brain tumors obtained during surgery and 15 normal brain tissues that were collected during autopsy. Results were compared between two groups. RESULTS: In general, protein oxidation and lipid peroxidation increased while antioxidant capacity decreased significantly in tumors compared to the controls (p<0...
May 3, 2017: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/28490767/biliverdin-reductase-inhibitors-did-not-improve-severe-unconjugated-hyperbilirubinemia-in-vivo
#13
Remco van Dijk, Sem J Aronson, Dirk R de Waart, Stan F van de Graaf, Suzanne Duijst, Jurgen Seppen, Ronald Oude Elferink, Ulrich Beuers, Piter J Bosma
We aimed to identify potent biliverdin reductase (BVRA) inhibitors as a novel concept for the treatment of severe unconjugated hyperbilirubinemia. 1280 FDA-approved compounds were screened in vitro for their ability to inhibit human and rat BVRA activity and 26 compounds were identified as BVRA inhibitors. Montelukast and Disulfiram were selected as potentially clinically applicable drugs and tested to reduce serum unconjugated bilirubin (UCB) levels in the Ugt1a1-deficient rat, a model for chronic unconjugated hyperbilirubinemia...
May 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28481303/interaction-of-biliverdin-chromophore-with-near-infrared-fluorescent-protein-bphp1-fp-engineered-from-bacterial-phytochrome
#14
Olesya V Stepanenko, Olga V Stepanenko, Irina M Kuznetsova, Daria M Shcherbakova, Vladislav V Verkhusha, Konstantin K Turoverov
Near-infrared (NIR) fluorescent proteins (FPs) designed from PAS (Per-ARNT-Sim repeats) and GAF (cGMP phosphodiesterase/adenylate cyclase/FhlA transcriptional activator) domains of bacterial phytochromes covalently bind biliverdin (BV) chromophore via one or two Cys residues. We studied BV interaction with a series of NIR FP variants derived from the recently reported BphP1-FP protein. The latter was engineered from a bacterial phytochrome RpBphP1, and has two reactive Cys residues (Cys15 in the PAS domain and Cys256 in the GAF domain), whereas its mutants contain single Cys residues either in the PAS domain or in the GAF domain, or no Cys residues...
May 8, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28480786/ultraviolet-a1-irradiation-therapy-for-systemic-lupus-erythematosus
#15
H McGrath
Systemic lupus erythematosus (lupus, SLE) is a chronic autoimmune disease characterized by the production of autoantibodies, which bind to antigens and are deposited within tissues to fix complement, resulting in widespread systemic inflammation. The studies presented herein are consistent with hyperpolarized, adenosine triphosphate (ATP)-deficient mitochondria being central to the disease process. These hyperpolarized mitochondria resist the depolarization required for activation-induced apoptosis. The mitochondrial ATP deficits add to this resistance to apoptosis and also reduce the macrophage energy that is needed to clear apoptotic bodies...
January 1, 2017: Lupus
https://www.readbyqxmd.com/read/28459399/-biliverdin-protects-the-isolated-lungs-from-ischemia-reperfusion-injury-via-anti-apoptosis
#16
Jieyun Sun, Peng Zhang, Xinying Yang, Di Xu, Bin Du, Junliang Chen, Minchen Wu, Qingfeng Pang
OBJECTIVE: To observe the effect of biliverdin (BV) on the lung ischemia/reperfusion injury (LIRI), and to investigate the mechanism of BV in treatment of LIRI. METHODS: Thirty-two male Sprague-Dawley (SD) rats were randomly divided into control group, LIRI group, glutathione (GSH) group and BV-treated group, with 8 rats in each group. The rat LIRI model was reproduced by isolated lung perfusion system. Fifteen minutes after perfusion balance, lungs of control group were perfused continuously for 90 minutes, and the lungs in other groups were reperfused for 90 minutes after 1-hour ischemia, while the perfusion was added 10 μmol/L BV in BV-treated group and 4 mmol/L GSH in GSH group respectively...
January 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28421060/the-heme-metabolite-carbon-monoxide-facilitates-kshv-infection-by-inhibiting-tlr4-signaling-in-endothelial-cells
#17
Sara Botto, Jean K Gustin, Ashlee V Moses
Kaposi sarcoma herpesvirus (KSHV) is the etiologic agent of Kaposi sarcoma (KS) and certain rare B cell lymphoproliferative disorders. KSHV infection of endothelial cells (EC) in vitro increases expression of the inducible host-encoded enzyme heme oxygenase-1 (HO-1), which is also strongly expressed in KS tumors. HO-1 catalyzes the rate-limiting step in the conversion of heme into iron, biliverdin and the gasotransmitter carbon monoxide (CO), all of which share anti-apoptotic, anti-inflammatory, pro-survival, and tumorigenic activities...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28412905/therapeutic-potential-of-heme-oxygenase-1-carbon-monoxide-system-against-ischemia-reperfusion-injury
#18
Yuanyuan Cheng, Jianhui Rong
BACKGROUND: Ischemia-reperfusion (I/R) injury causes the dysfunctions of different major organs, leading to morbidity and mortality on the global scale. Among a battery of therapeutic targets, the heme oxygenase-1 (HO-1)/carbon monoxide (CO) system has been evaluated for the development of new therapies against I/R injury. The enzyme HO-1 catalyzes the degradation of heme into three biologically active end products, namely biliverdin/bilirubin, CO and ferrous ion. Interestingly, CO is one of a few bioactive gaseous molecules with the capability of regulating inflammation, cell survival and growth...
April 13, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28412296/impact-of-higher-order-heme-degradation-products-on-hepatic-function-and-hemodynamics
#19
Raphael A Seidel, Thierry Claudel, Franziska A Schleser, Navin K Ojha, Matthias Westerhausen, Sandor Nietzsche, Christoph Sponholz, Frans Cuperus, Sina M Coldewey, Stefan H Heinemann, Georg Pohnert, Michael Trauner, Michael Bauer
BACKGROUND& AIMS: While, traditionally, biliverdin and bilirubin are considered end products of heme catabolism, there is evidence for further degradation to diverse bioactive products. As such, Z-BOX A and Z-BOX B arise upon oxidation with unknown implications for hepatocellular function and integrity. We studied the impact of Z-BOX A and B on hepatic functions and explored their alterations in health and cholestatic conditions. METHODS: Functional implications and mechanisms were investigated in rats, hepatocytic HepG2 and HepaRG cells, human immortalized hepatocytes, and isolated perfused livers...
April 12, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/28401765/near-infrared-fluorescent-proteins-biosensors-and-optogenetic-tools-engineered-from-phytochromes
#20
REVIEW
Konstantin G Chernov, Taras A Redchuk, Evgeniya S Omelina, Vladislav V Verkhusha
Phytochrome photoreceptors absorb far-red and near-infrared (NIR) light and regulate light responses in plants, fungi, and bacteria. Their multidomain structure and autocatalytic incorporation of linear tetrapyrrole chromophores make phytochromes attractive molecular templates for the development of light-sensing probes. A subclass of bacterial phytochromes (BphPs) utilizes heme-derived biliverdin tetrapyrrole, which is ubiquitous in mammalian tissues, as a chromophore. Because biliverdin possesses the largest electron-conjugated chromophore system among linear tetrapyrroles, BphPs exhibit the most NIR-shifted spectra that reside within the NIR tissue transparency window...
May 10, 2017: Chemical Reviews
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