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Batu Aydinuraz, Enver Kerem Dirican, Safak Olgan, Ozlem Aksunger, Onur Kadir Erturk
This study aimed to evaluate the effect of artificial oocyte activation (AOA) by calcium ionophore after intracytoplasmic morphologically selected sperm injection (IMSI) on fertilization, cleavage rate and embryo quality. A total of 194 oocytes from 21 cycles from women with a history of low fertilization rate accompanying teratozoospermia were enrolled over a 3-month period. Mature oocytes from each patient were randomly allocated into two groups after IMSI. In the study group, half of the patients' oocytes (n = 97) were exposed to AOA, and in the control group (n = 97), AOA was not applied...
October 13, 2016: Human Fertility: Journal of the British Fertility Society
Mara Simopoulou, Laertis Gkoles, Panagiotis Bakas, Polina Giannelou, Theodoros Kalampokas, Konstantinos Pantos, Michael Koutsilieris
: Intracytoplasmic sperm injection (ICSI) is the most frequently applied method for fertilization making the process of identifying the perfect spermatozoon fundamental. Herein we offer a critical and thorough presentation on the techniques reported regarding (i) handling and preparing semen samples, (ii) identifying and 'fishing' spermatozoa, and (iii) improving key factors, such as motility for a successful ICSI practice. These approaches are suggested to make the process easier and more effective especially in atypical and challenging circumstances...
September 20, 2016: Systems Biology in Reproductive Medicine
A Hershko-Klement, R Sukenik-Halevy, T Biron Shental, N Miller, A Berkovitz
Our objective was to study the birth defect rates in intracytoplasmic morphologically selected sperm injection (IMSI) pregnancies. A cohort of couples presenting male factor infertility between January 2006 and January 2014 was retrospectively analyzed. Discharge letters and a telephone interview were performed for assessing pregnancy outcome. All clinical data were reviewed by a board certified medical geneticist. Main outcomes were fetal/birth defect and chromosomal abnormality rates. Two thousand two hundred and fifty-eight pregnancies were available for analysis, of them, 1669 (73...
September 2016: Andrology
C K Bradley, S J McArthur, A J Gee, K A Weiss, U Schmidt, L Toogood
Sperm DNA fragmentation (SDF) is used in assisted reproductive technology (ART) programs as an indicator for sperm quality, although there is still a lack of consensus as to its clinical utility. In this retrospective study, we examined intracytoplasmic sperm injection (ICSI) outcomes of 1924 infertile patients who underwent SDF analysis using the sperm chromatin integrity test. ART patients were classified as having low [DNA fragmentation index (DFI) <29%] or high SDF (DFI ≥29%) and by whether or not an intervention [physiological intracytoplasmic sperm injection (PICSI), intracytoplasmic morphologically selected sperm injection (IMSI), testicular sperm extraction (TESE)/testicular sperm aspiration (TESA), frequent ejaculation] was performed...
September 2016: Andrology
Elena Moretti, Gaetano Sutera, Giulia Collodel
UNLABELLED: This review is aimed at discussing the role of ultrastructural studies on human spermatozoa and evaluating transmission electron microscopy as a diagnostic tool that can complete andrology protocols. It is clear that morphological sperm defects may explain decreased fertilizing potential and acquire particular value in the field of male infertility. Electron microscopy is the best method to identify systematic or monomorphic and non-systematic or polymorphic sperm defects...
June 2016: Systems Biology in Reproductive Medicine
Daniel Luna, Roly Hilario, Julio Dueñas-Chacón, Rocío Romero, Patricia Zavala, Lucy Villegas, Javier García-Ferreyra
PURPOSE: The intracytoplasmic morphologically selected sperm injection (IMSI) procedure has been associated with better laboratory and clinical outcomes in assisted reproduction technologies. Less information is available regarding the relationship between embryo aneuploidy rate and the IMSI procedure. The aim of this study is to compare the clinical outcomes and chromosomal status of IMSI-derived embryos with those obtained from intracytoplasmic sperm injection (ICSI) in order to establish a clearer view of the benefits of IMSI in infertile patients...
2015: Clinical Medicine Insights. Reproductive Health
Yi Gong, Xiang-Qian Meng, Mian Liu, Jing Wang, Fu Xiong, Ying Zhong, Song Quan
OBJECTIVE: To test whether intracytoplasmic injection of morphologically selected spermatozoa (IMSI) from patients with male factor infertility can improve the clinical and embryo development outcomes of intracytoplasmic sperm injection-embryo transfer (ICSI-ET). METHODS: We performed IMSI for 82 couples diagnosed with obstructive azoospermia at high magnification (×6600) and traditional ICSI for another 91 couples using testicular sperms. We also performed IMSI for 44 couples with teratozoospermia at high magnification (×6600) and traditional ICSI for 71 patients using ejaculated sperms...
October 2015: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
L Chansel-Debordeaux, S Dandieu, S Bechoua, C Jimenez
Infertility affects approximately 15% of couples in reproductive age. Male infertility is estimated to represent about 20% of the etiologies. Among them, a rare type of teratozoospermia known as globozoospermia leads to disappointing pregnancy outcomes. Morphological, physiological and genetic aspects of this severe disorder have been described. We undertook a complete review of the available data on the reproductive outcomes in globozoospermic patients. To this end, a literature review in both English and French, over a 20-year time period using PubMed/Medline, ScienceDirect, and Scopus was performed...
November 2015: Andrology
James D M Nicopoullos, E Whitney, V Wells, S Batha, R Faris, H Abdalla
PURPOSE: Total fertilisation failure (TFF), even with intracytoplasmic sperm injection (ICSI), occurs in approximately 3 % of cycles, can be recurrent and the exact cause is difficult to elucidate. Differentiation between oocyte and sperm-related cause of TFF is possible using mouse oocyte-activation techniques, but is not an option within most clinical settings. Therefore, the management of these couples is clinically driven, and the endpoint, if recurrent, is often the use of donor gametes...
November 2015: Journal of Assisted Reproduction and Genetics
Roy Amit, Shlomit Greenberg
PURPOSE: Our eyes are in constant motion. Even when fixating, our eyes produce small saccades called microsaccades. Following a visual stimulus onset, microsaccade rate follows a consistent modulation of a decrease and then increase. However, much less is known on the effect of ocular events on the occurrence of consecutive microsaccades. It was previously suggested that the process triggering microsaccades is a Poisson process, and therefore the inter-microsaccade intervals (IMSIs) follow an exponential distribution...
2015: Journal of Vision
Giovanni Battista La Sala, Alessia Nicoli, Eleonora Fornaciari, Angela Falbo, Ilaria Rondini, Daria Morini, Barbara Valli, Maria Teresa Villani, Stefano Palomba
BACKGROUND: Intracytoplasmic morphologically selected sperm injection (IMSI) is still proposed and employed in the clinical practice to improve the reproductive outcome in infertile couples scheduled for conventional intracytoplasmic sperm injection (cICSI). The aim of the current randomized controlled trial (RCT) was to test the hypothesis that IMSI gives a better live birth delivery rate than cICSI. METHODS: Infertile couples scheduled for their first cICSI cycle for male factor were allocated using a simple randomization procedure...
2015: Reproductive Biology and Endocrinology: RB&E
Anton Neyer, Martin Zintz, Astrid Stecher, Magnus Bach, Barbara Wirleitner, Nicolas H Zech, Pierre Vanderzwalmen
PURPOSE: Various time-lapse studies have postulated embryo selection criteria based on early morphokinetic markers. However, late paternal effects are mostly not visible before embryonic genome activation. The primary objective of this retrospective study was to investigate whether those early morphokinetic algorithms investigated by time-lapse imaging are reliable enough to allow for the accurate selection of those embryos that develop into blastocysts, while of course taking into account the correlation with the type of injected spermatozoa...
November 2015: Journal of Assisted Reproduction and Genetics
Bogdan Doroftei, Mihaela Zlei, Gabriela Simionescu, Radu Maftei, Simona Cumpata, Geraldine Emerson
We report a successful ongoing pregnancy obtained in a case of total globozoospermia after intracytoplasmic morphologically selected sperm injection (IMSI) with oocyte activation. The first semen analysis on investigation showed partial globozoospermia. However, under high magnification assessment at oocyte retrieval only round headed sperm were observed. Considering the high risk of a complete failure to fertilize from IMSI the couple gave written informed consent to the use of oocyte activation media post IMSI...
2015: Reproductive Health
Ruth van Veelen, Sabine Otten, Mara Cadinu, Nina Hansen
Social identification denotes individuals' psychological bond with their ingroup. It is an indispensable construct in research on intragroup and intergroup dynamics. Today's understanding of social identification is firmly grounded in self-stereotyping principles (i.e., assimilation to the ingroup prototype). However, we argue for a more integrative approach to understand social identification, including a more prominent role for the personal self. We present the Integrative Model of Social Identification (IMSI) and postulate that there are two cognitive pathways to self-group overlap that can simultaneously yet distinctly explain social identification: self-stereotyping and self-anchoring (i...
February 2016: Personality and Social Psychology Review
Anick De Vos, Nikolaos P Polyzos, Greta Verheyen, Herman Tournaye
Introduced in 2001, intracytoplasmic morphologically selected sperm injection (IMSI) represents a more sophisticated way of ICSI whereby, prior to injection, the spermatozoon is selected at higher magnification. Doing so, the spermatozoon can be evaluated for fine integrity of its nucleus and the injection of a normal spermatozoon with a vacuole-free head can be assured. Additional research is needed to unravel the underlying mechanisms responsible for the presence of vacuoles in sperm heads. Associations with acrosome status, chromatin condensation, DNA fragmentation and sperm aneuploidy have been documented, however, controversy on their nature exists...
2013: Basic and Clinical Andrology
Amanda Souza Setti, Daniela Paes Almeida Ferreira Braga, Rita Cassia Sávio Figueira, Assumpto Iaconelli, Edson Borges
PURPOSE: To compare the outcomes of ICSI and IMSI in women presenting with poor ovarian response. METHODS: Data of IMSI cycles performed from January 2011 to December 2013 were included in this retrospective cohort study. Patients were divided into two groups: normoresponder patients (NR group; patients with > 4 oocytes retrieved) and poor-responder patients (PR group; patients with ≤ 4 oocytes retrieved). Patients who underwent IMSI were matched with patients who underwent ICSI in the same period...
March 2015: Journal of Assisted Reproduction and Genetics
Brian Dale, Yves Menezo, Gianfranco Coppola
Morphological selection techniques of gametes and embryos are of current interest to clinical practice in ART. Although intracytoplasmic morphologically selected sperm injection (IMSI), time lapse imaging morphometry (TLIM) or quantification of chromosome numbers (PGS) are potentially useful in research, they have not been shown to be of statistically predictive value and, thus, have only limited clinical usefulness. We make the point that morphological markers alone cannot predict the success of the early embryo, which depends on the correct orchestration of a myriad of physiological and biochemical activation events that progress independently of the maternal or zygotic genome...
April 2015: Journal of Assisted Reproduction and Genetics
Amanda S Setti, Daniela P A F Braga, Rita C S Figueira, Assumpto Iaconelli, Edson Borges
The objective of this study was to perform the first meta-analysis to compare conventional intracytoplasmic sperm injection (ICSI) outcomes and intracytoplasmic morphologically selected sperm injection (IMSI) outcomes in couples with previous ICSI failures (IF) or male factor infertility (MF). A systematic review was performed by searching Medline database to identify articles reporting on the comparison between ICSI and IMSI outcomes in couples with IF or MF. The main outcome measures were the implantation, pregnancy and miscarriage rates...
December 2014: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Einat Shalom-Paz, Saja Anabusi, Medeia Michaeli, Etti Karchovsky-Shoshan, Nechami Rothfarb, Tal Shavit, Adrian Ellenbogen
OBJECTIVE: Spermatozoal morphology was reported to effect fertilization, embryo quality and pregnancy results in spontaneous conception and ART. Intracytoplasmic morphologically selected sperm injection (IMSI) is an innovative, not invasive technique, which examines the sperm with no harm at a magnification of 6000 × in order to obtain optimal sperm to perform IVF-ICSI. We evaluated the efficiency of IMSI technique in patients with repeated IVF-ICSI failure of at least three cycles with no viable pregnancy and/or very poor sperm quality...
March 2015: Gynecological Endocrinology
Michelle M Oakes, Lisa K Baxter, Rachelle M Duvall, Meagan Madden, Mingjie Xie, Michael P Hannigan, Jennifer L Peel, Jorge E Pachon, Siv Balachandran, Armistead Russell, Thomas C Long
A variety of single pollutant and multipollutant metrics can be used to represent exposure to traffic pollutant mixtures and evaluate their health effects. Integrated mobile source indicators (IMSIs) that combine air quality concentration and emissions data have recently been developed and evaluated using data from Atlanta, Georgia. IMSIs were found to track trends in traffic-related pollutants and have similar or stronger associations with health outcomes. In the current work, we apply IMSIs for gasoline, diesel and total (gasoline + diesel) vehicles to two other cities (Denver, Colorado and Houston, Texas) with different emissions profiles as well as to a different dataset from Atlanta...
November 2014: International Journal of Environmental Research and Public Health
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