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https://www.readbyqxmd.com/read/28428442/combination-of-ibrutinib-and-abt-199-in-diffuse-large-b-cell-lymphoma-and-follicular-lymphoma
#1
Hsu-Ping Kuo, Scott A Ezell, Karl J Schweighofer, Leo Wk Cheung, Sidney Hsieh, Mutiah Apatira, Mint Sirisawad, Karl Eckert, Ssucheng J Hsu, Chun-Te Chen, Darrin M Beaupre, Matthias Versele, Betty Y Chang
Diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma (FL) are the most prevalent B-lymphocyte neoplasms in which abnormal activation of the Bruton's tyrosine kinase (BTK)-mediated B-cell receptor (BCR) signaling pathway contributes to pathogenesis. Ibrutinib is an oral covalent BTK inhibitor that has shown some efficacy in both indications. To improve ibrutinib efficacy through combination therapy, we first investigated differential gene expression in parental and ibrutinib-resistant cell lines to better understand the mechanisms of resistance...
April 20, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/28425857/antioxidant-properties-of-fractions-for-unripe-fruits-of-capsicum-annuum-l-var-conoides
#2
Chung-Yi Chen, Ching-Yu Yen, Gao-Mai Shen, Tzu-Jung Yu, Yi-Shin Liao, Ru-In Jian, Sheng-Chieh Wang, Jen-Yang Tang, H-W Chang
BACKGROUND: Capsicum plant, especially for C. annuum, is an abundant resource for bioactive antioxidants, but few studies have examined the unripe fruit part of the Capsicum plant. OBJECTIVE: MeOH extract of unripe fruits of C. annuum L. var. conoides (UFCA) was chromatographed over a silica gel column using a gradient of CH2Cl2/MeOH as eluent to produce 9 fractions. Antioxidant activities are evaluated along with cell viabilities of 9 fractions of UFCA. METHOD: The antioxidant properties were analyzed in terms of total phenol content (TPC), total flavonoid content (TFC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, ferric reducing, and ferrous ion-chelating ability...
April 19, 2017: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/28424722/the-role-of-auxin-ethylene-crosstalk-in-orchestrating-primary-root-elongation-in-sugar-beet
#3
Willem Abts, Bert Vandenbussche, Maurice P De Proft, Bram Van de Poel
It is well-established in Arabidopsis and other species that ethylene inhibits root elongation through the action of auxin. In sugar beet (Beta vulgaris L.) ethylene promotes root elongation in a concentration dependent manner. However, the crosstalk between ethylene and auxin remains unknown during sugar beet seedling development. Our experiments have shown that exogenously applied auxin (indole-3-acetic acid; IAA) also stimulates root elongation. We also show that auxin promotes ethylene biosynthesis leading to longer roots...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28423872/potential-antioxidant-and-lipid-peroxidation-inhibition-of-phyllanthus-acidus-leaf-extract-in-minced-pork
#4
Tuyen Thi Kim Nguyen, Chamroon Laosinwattana, Montinee Teerarak, Komkhae Pilasombut
Objective: This study was to investigate the effect of extraction solvents on antioxidant bio-active compounds as well as potential antioxidant and lipid peroxidation inhibition of Phyllanthus acidus leaf extract in minced pork. Methods: The effect of various solvent systems of water, 25%, 50%, 75% (v/v) ethanol in water and absolute ethanol on the extraction crude yield, total phenolic content, total flavonoid content and in vitro antioxidant activities of P. acidus leaves was determined...
April 19, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28423605/pregnancy-at-early-age-is-associated-with-a-reduction-of-progesterone-responsive-cells-and-epithelial-wnt-signaling-in-human-breast-tissue
#5
Simone Muenst, Robert Mechera, Silvio Däster, Salvatore Piscuoglio, Charlotte K Y Ng, Fabienne Meier-Abt, Walter P Weber, Savas D Soysal
BACKGROUND: Pregnancy at early age is the most significant modifiable factor which consistently decreases lifetime breast cancer risk. However, the underlying mechanisms haven't been conclusively identified. Studies in mice suggest a reduction in progesterone-receptor (PR) sensitive epithelial cells as well as a downregulation of the Wnt signaling pathway as being one of the main mechanisms for the protective effect of early pregnancy. The aim of our study was to validate these findings in humans...
April 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28423086/evaluation-of-cytogenotoxicity-antioxidant-and-hypoglycemiant-activities-of-isolate-compounds-from-mansoa-hirsuta-d-c-bignoniaceae
#6
Joquebede R Pereira, Raphael F Queiroz, Erlânia A DE Siqueira, Ana Christina Brasileiro-Vidal, Antônio E G Sant'ana, Daniel M Silva, Paulo R A DE Mello Affonso
Mansoa hirsuta (Bignoniaceae) is a native plant from caatinga in Brazilian semiarid. This plant has been locally used as antimicrobial and hypoglycemiant agents, but their action mechanisms and toxicity remain largely unknown. Therefore, we evaluated the composition and antioxidant, cytoprotective and hypoglycemiant effects of raw extract, fractions and compounds from leaves of M. hirsuta. The cytogenotoxic effects of ursolic and oleanolic acids, the main phytotherapic components of this plant, were assessed...
January 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28421300/functional-teas-from-the-leaves-of-arbutus-unedo-phenolic-content-antioxidant-activity-and-detection-of-efficient-radical-scavengers
#7
Ioannis Erkekoglou, Nikolaos Nenadis, Efrosini Samara, Fani Th Mantzouridou
The phenolic content/composition and antioxidant activity of hot/cold infusion and decoction from the leaves of Arbutus unedo were studied for the first time. 1,1-diphenyl-2-picrylhydrazyl (DPPH(●)), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical cation (ABTS(●+)), crocin-bleaching, copper-reducing, and liposome accelerated oxidation assays were used for the evaluation of the activity in vitro. In vivo, the extracts were examined for their ability to protect S. cerevisiae cells from H2O2 induced oxidative stress...
April 18, 2017: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/28421142/plackett-burman-design-for-rgilcc1-laccase-activity-enhancement-in-pichia-pastoris-concentrated-enzyme-kinetic-characterization
#8
Edwin D Morales-Álvarez, Claudia M Rivera-Hoyos, Ángela M Cardozo-Bernal, Raúl A Poutou-Piñales, Aura M Pedroza-Rodríguez, Dennis J Díaz-Rincón, Alexander Rodríguez-López, Carlos J Alméciga-Díaz, Claudia L Cuervo-Patiño
Laccases are multicopper oxidases that catalyze aromatic and nonaromatic compounds with concomitant reduction of molecular oxygen to water. They are of great interest due to their potential biotechnological applications. In this work we statistically improved culture media for recombinant GILCC1 (rGILCC1) laccase production at low scale from Ganoderma lucidum containing the construct pGAPZαA-GlucPost-Stop in Pichia pastoris. Temperature, pH stability, and kinetic parameter characterizations were determined by monitoring concentrate enzyme oxidation at different ABTS substrate concentrations...
2017: Enzyme Research
https://www.readbyqxmd.com/read/28419463/profiling-of-anthocyanins-in-transgenic-purple-fleshed-sweet-potatoes-by-hplc-ms-ms
#9
Jingqiu Ge, Yijie Hu, Hongxia Wang, Yuanshe Huang, Peng Zhang, Zhihua Liao, Min Chen
BACKGROUND: Anthocyanins in Purple-fleshed Sweet Potato (PSP) are beneficial to human health. The leaf color (Lc) gene is a transcription factor involved in regulating anthocyanin biosynthesis. The anthocyanin profiles of wild-type PSP of Ayamurasaki and its three Lc-transgenic lines were investigated by HPLC-MS/MS. And in vitro antioxidant activities of wild-type and Lc-transgenic lines, including reducing power activity, DPPH radical scavenging activity, hydroxyl radical scavenging activity, linoleic acid autoxidation inhibition activity, ABTS free radical scavenging activity and oxygen radical absorbance capacity activity, were measured...
April 17, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28417084/antioxidant-antimicrobial-activity-and-bioactive-compounds-of-bergenia-ciliata-sternb-a-valuable-medicinal-herb-of-sikkim-himalaya
#10
Mithilesh Singh, Neha Pandey, Vasudha Agnihotri, K K Singh, Anita Pandey
Bergenia ciliata Sternb., commonly known as Paashaanbhed, is a well known herb of Sikkim Himalaya with various pharmaceutical properties. However, scientific exploration of B. ciliata, growing in the Sikkim Himalaya, for phytochemicals and pharmacological properties is in infancy. With this view, the present study was undertaken to investigate B. ciliata leaf extracts for antioxidant, antimicrobial activity and bioactive compounds. Three solvents viz., methanol, ethyl acetate and hexane were used for extraction and the respective leaf extracts were analyzed for total phenolic and flavonoid contents along with the antioxidant and antimicrobial activities...
April 2017: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/28416338/separation-and-purification-of-polyphenols-from-red-wine-extracts-using-high-speed-counter-current-chromatography
#11
Yuanyuan Li, Lingxi Li, Yan Cui, Shuting Zhang, Baoshan Sun
Polyphenols are important compounds of red wine owing to their contribution to sensory properties and antioxidant activities. In this study, high-speed counter-current chromatography (HSCCC) coupled with semi-preparative HPLC was used for large-scale separation and purification of polyphenols from red wine extracts. With the solvent system of hexane-ethyl acetate-water (1-50-50), various oligomeric procyanidins including monomer catechin, epicatechin, dimers B1, B2; phenolic acids including coutaric acid, caftaric acid and other type of polyphenols were largely separated within 370min and most of these compounds presented high yields (0...
March 12, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28415842/spectrophotometric-quantification-of-peroxidase-with-p-phenylene-diamine-for-analyzing-peroxidase-encapsulating-lipid-vesicles
#12
Ya Zhang, Yannick R F Schmid, Sandra Luginbühl, Qiang Wang, Petra S Dittrich, Peter Walde
A spectrophotometric assay for the determination of horseradish peroxidase (HRP) in aqueous solution with p-phenylenediamine (PPD, benzene-1,4-diamine) as electron donor substrate and hydrogen peroxide (H2O2) as oxidant was developed. The oxidation of PPD by HRP/H2O2 leads to the formation of Bandrowski's base ((3E,6E)-3,6-bis[(4-aminophenyl)imino]cyclohexa-1,4-diene-1,4-diamine), which can be quantified by following the increase in absorbance at 500 nm. The assay was applied for monitoring the activity of HRP inside ≈180 nm-sized lipid vesicles (liposomes), prepared from POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) and purified by size exclusion chromatography...
April 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28415001/degradation-of-synthetic-fragrances-by-laccase-mediated-system
#13
Laura Vallecillos, Yumna Sadef, Francesc Borrull, Eva Pocurull, Kai Bester
Laccase mediator systems are important biodegradation agents as the rate of reaction could be enhanced in the presence of redox mediators. In the present study the commercial enzyme laccase from Trametes versicolor and the redox mediator 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were used for the biotransformation of the synthetic fragrances 1-(1,2,3,4,5,6,7,8-octahydro-2,3,8,8,-tetramethyl-2-naphthyl)ethan-1-one (Iso-E-Super, OTNE), 1,3,4,6,7,8,-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta-[g]-2-benzopyran (Galaxolide, HHCB), 7-acetyl-1,1,3,4,4,6-hexamethyl-1,2,3,4-tetrahydronaphtalene (Tonalide, AHTN) and the transformation product of HHCB, 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta-[g]-2-benzopyran-1-one (Galaxolidone, HHCB-lactone) in water...
April 4, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28412293/glecaprevir-and-pibrentasvir-yield-high-response-rates-in-patients-with-hcv-genotype-1-6-without-cirrhosis
#14
Paul Y Kwo, Fred Poordad, Armen Asatryan, Stanley Wang, David L Wyles, Tarek Hassanein, Franco Felizarta, Mark S Sulkowski, Edward Gane, Benedict Maliakkal, J Scott Overcash, Stuart C Gordon, Andrew J Muir, Humberto Aguilar, Kosh Agarwal, Gregory J Dore, Chih-Wei Lin, Ran Liu, Sandra S Lovell, Teresa I Ng, Jens Kort, Federico J Mensa
BACKGROUND: and Aims Hepatitis C virus (HCV) therapy that is highly efficacious, pangenotypic, with a high barrier to resistance and short treatment duration is desirable. The efficacy and safety of 8- and 12-week treatments with glecaprevir (ABT-493; NS3/4A protease inhibitor) and pibrentasvir (ABT-530; NS5A inhibitor) was evaluated in non-cirrhotic patients with chronic HCV genotype 1-6 infection. METHODS: SURVEYOR-I and SURVEYOR-II were phase 2, open-label, multicenter, dose-ranging trials including patients with chronic HCV genotype 1-6 infection who were either previously untreated or treated with pegylated interferon plus ribavirin...
April 12, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/28411365/analysis-of-glycosylated-flavonoids-extracted-from-sweet-cherry-stems-as-antibacterial-agents-against-pathogenic-escherichia-coli-isolates
#15
Alfredo Aires, Carla Dias, Rosa Carvalho, Maria José Saavedra
The aim of this study was to evaluate the bioactivity of flavonoids extracted from sweet-cherry stems which are often used by a traditional system of medicine to treat gastro-intestinal and urinary tract infections but lacking any consistent scientific evidence; moreover the information about the class of phenolics, their content and the potential bioactivity of such material is very scarce. Thus, in this context, we have set a research study in which we evaluated the profile and content of phenolics extracted from sweet-cherry stems through a conventional (70ºC and 20 min) and ultrasound assisted extraction (40 kHz, room temperature and 20 min)...
April 14, 2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28407975/chemical-composition-of-lycium-europaeum-fruit-oil-obtained-by-supercritical-co2-extraction-and-evaluation-of-its-antioxidant-activity-cytotoxicity-and-cell-absorption
#16
Antonella Rosa, Andrea Maxia, Danilo Putzu, Angela Atzeri, Benedetta Era, Antonella Fais, Cinzia Sanna, Alessandra Piras
We studied the total phenols and flavonoids, liposoluble antioxidants, fatty acid and triacylglycerol profiles, and oxidative status of oil obtained from Lycium europaeum fruits following supercritical CO2 extraction (at 30MPa and 40°C). Linoleic (52%), palmitic (18%), oleic (13%), and α-linolenic (6%) were the main oil fatty acids, while trilinolein and palmitodilinolein/oleodilinolein represented the main triacylglycerols. The oil was characterized by high levels of all-trans-zeaxanthin and all-trans-β-carotene (755 and 332μg/g of oil, respectively), α-tocopherol (308μg/g of oil), total phenols (13...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28407907/the-effects-of-postharvest-application-of-lecithin-to-improve-storage-potential-and-quality-of-fresh-goji-lycium-barbarum-l-berries
#17
Mushtaque Ahmed Jatoi, Slaven Jurić, Rajko Vidrih, Marko Vinceković, Marko Vuković, Tomislav Jemrić
To enhance storage life and post-storage quality of fresh goji berries, three treatments with lecithin (1, 5, 10g·L(-1)) and two storage times (8, 16days) were evaluated. The significant effects on the physiological and biochemical parameters were varied. 1g·L(-1) lecithin showed its main effects after 8days of storage by reduction in total weight loss and decay, SSC/TA ratio (also at 16days), and chlorophyll content and with highest scores of sensory attributes (also at 16days). 5g·L(-1) lecithin showed its main effects after 16days of storage: highest SSC, highest TA (also at 8days), highest TPC, only significant reduction in DPPH antioxidant activity, and highest total flavonoid content...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28407893/h2o2-oxidative-preparation-characterization-and-antiradical-activity-of-a-novel-oligosaccharide-derived-from-flaxseed-gum
#18
Shan Liang, Wenzhen Liao, Xiang Ma, Xiaofeng Li, Yong Wang
In this present study, a novel flaxseed gum oligosaccharide (FGOS) was prepared by H2O2 oxidative degradation method followed by physicochemical properties characterization and antiradical activity evaluation. Results indicated that the degradation rate of flaxseed gum is 37.81% under the optimum conditions (i.e., reaction temperature of 120°C, H2O2 concentration of 0.2M and reaction time of 2.0h) and FGOS as a reddish brown semisolid was obtained. Physicochemical properties identification showed that FGOS has a molecular weight of 1047Da and is a typical oligosaccharide which contains uronic acid...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28407721/chemical-composition-and-biological-investigation-of-pelargonium-endlicherianum-root-extracts
#19
Gökçe Şeker Karatoprak, Fatih Göger, Mükerrem Betül Yerer, Müberra Koşar
CONTEXT: Pelargonium endlicherianum Fenzl. (Geraniaceae) roots and flowers are traditionally used in Turkey as a decoction treatment against intestinal parasites. Neither the chemical composition nor the potential bioactivity of the plant roots has been studied before. OBJECTIVES: The phenolic content and effects of P. endlicherianum root extracts on antioxidant enzyme levels on A549 cells were studied for the first time. MATERIALS AND METHODS: The chemical composition was analyzed via spectrophotometric and chromatographic (HPLC MS/MS and HPLC) techniques...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28407441/same-policy-different-impact-center-level-effects-of-share-35-liver-allocation
#20
Douglas R Murken, Allison W Peng, David D Aufhauser, Peter L Abt, David S Goldberg, Matthew H Levine
Early studies of national data suggest that the Share 35 allocation policy increased liver transplants without compromising post-transplant outcomes. Changes in center-specific volumes and practice patterns in response to the national policy change are not well characterized. Understanding center-level responses to Share 35 is crucial for optimizing the policy and constructing effective future policy revisions. Data from the United Network for Organ Sharing were analyzed to compare center-level volumes of allocation-MELD ≥35 transplants pre- and post-policy implementation...
April 13, 2017: Liver Transplantation
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