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https://www.readbyqxmd.com/read/29156758/development-of-novel-long-noncoding-rna-malat1-near-infrared-optical-probes-for-in-vivo-tumour-imaging
#1
Meng-Jie Dong, Cai-Qin Wang, Guo-Lin Wang, Yue-Hong Wang, Zhen-Feng Liu
With the advent of next-generation sequencing technology, there is rapidly increasing interest in long noncoding RNAs (lncRNAs). The objectives of this study were to develop a novel lncRNA MALAT1 near-infrared optical probe, to evaluate the characteristics of this optical imaging probe in vitro and to determine whether it can be used for imaging MALAT1 expression in malignant tumours in vivo. Conjugation of Cy5.5 to MALAT1 ASO was accomplished using standard NHS (N-hydroxysuccinimide) ester procedures, and the labelled MALAT1 ASO was purified with a Glen-Pak DNA Purification Cartridge and reversed-phase high performance liquid chromatography (HPLC)...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29155829/environmental-and-socio-demographic-individual-family-and-neighborhood-factors-associated-with-children-intestinal-parasitoses-at-iguaz%C3%A3%C2%BA-in-the-subtropical-northern-border-of-argentina
#2
Maria Romina Rivero, Carlos De Angelo, Pablo Nuñez, Martín Salas, Carlos E Motta, Alicia Chiaretta, Oscar D Salomón, Song Liang
BACKGROUND: Intestinal parasitoses are a major concern for public health, especially in children from middle and low-income populations of tropical and subtropical areas. We examined the presence and co-infection of parasites in humans as well as parasitic environmental contamination in Puerto Iguazú, Argentina. We explored the environmental and socio-demographic characteristics of the persistence of parasites in children and their environment. METHODOLOGY/PRINCIPAL FINDINGS: This cross-section survey was conducted among children population comprised into the area of the public health care centers of Iguazú during June 2013 to May 2016...
November 20, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29155773/dna-magnetic-particle-binding-analysis-by-dynamic-and-electrophoretic-light-scattering
#3
Yazan Haddad, Simona Dostalova, Jiri Kudr, Ondrej Zitka, Zbynek Heger, Vojtech Adam
Isolation of DNA using magnetic particles is a field of high importance in biotechnology and molecular biology research. This protocol describes the evaluation of DNA-magnetic particles binding via dynamic light scattering (DLS) and electrophoretic light scattering (ELS). Analysis by DLS provides valuable information on the physicochemical properties of particles including particle size, polydispersity, and zeta potential. The latter describes the surface charge of the particle which plays major role in electrostatic binding of materials such as DNA...
November 9, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155707/optimized-lc-ms-ms-method-for-the-high-throughput-analysis-of-clinical-samples-of-ivacaftor-its-major-metabolites-and-lumacaftor-in-biological-fluids-of-cystic-fibrosis-patients
#4
Elena K Schneider, Felisa Reyes-Ortega, Jian Li, Tony Velkov
Defects in the cystic fibrosis trans-membrane conductance regulator (CFTR) are the cause of cystic fibrosis (CF), a disease with life-threatening pulmonary manifestations. Ivacaftor (IVA) and ivacaftor-lumacaftor (LUMA) combination are two new breakthrough CF drugs that directly modulate the activity and trafficking of the defective CFTR-protein. However, there is still a dearth of understanding on pharmacokinetic/pharmacodynamic parameters and the pharmacology of ivacaftor and lumacaftor. The HPLC-MS technique for the simultaneous analysis of the concentrations of ivacaftor, hydroxymethyl-ivacaftor, ivacaftor-carboxylate, and lumacaftor in biological fluids in patients receiving standard ivacaftor or ivacaftor-lumacaftor combination therapy has previously been developed by our group and partially validated to FDA standards...
October 15, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155642/extracting-regional-oxygen-tension-from-multibreath-wash-in-3-he-mr-imaging
#5
Harald E Möller
No abstract text is available yet for this article.
December 2017: Radiology
https://www.readbyqxmd.com/read/29155632/regional-fractional-ventilation-by-using-multibreath-wash-in-3-he-mr-imaging
#6
Hooman Hamedani, Justin T Clapp, Stephen J Kadlecek, Kiarash Emami, Masaru Ishii, Warren B Gefter, Yi Xin, Maurizio Cereda, Hoora Shaghaghi, Sarmad Siddiqui, Milton D Rossman, Rahim R Rizi
No abstract text is available yet for this article.
December 2017: Radiology
https://www.readbyqxmd.com/read/29155281/effect-of-oregano-essential-oil-and-carvacrol-on-cryptosporidium-parvum-infectivity-in-hct-8-cells
#7
Shashank Gaur, Theresa B Kuhlenschmidt, Mark S Kuhlenschmidt, Juan E Andrade
Cryptosporidium parvum is the second leading cause of persistent diarrhea among children in low-resource settings. This study examined the effect of oregano essential oil (OEO) and carvacrol (CV) on inhibition of C. parvum infectivity in vitro. HCT-8 cells were seeded (1×10(6)) in 96-well microtiter plates until confluency. Cell viability and infectivity were assessed by seeding HCT-8 cell monolayers with C. parvum oocysts (1×10(4)) in two modalities: 1) 4h co-culture with bioactive (0-250μg/mL) followed by washing and incubation (48h, 37°C, 5% CO2) in bioactive-free media; and 2) 4h co-culture of C...
November 15, 2017: Parasitology International
https://www.readbyqxmd.com/read/29154799/integrative-omics-data-analyses-of-repeated-dose-toxicity-of-valproic-acid-in-vitro-reveal-new-mechanisms-of-steatosis-induction
#8
Simone G J van Breda, Sandra M H Claessen, Marcel van Herwijnen, Daniël H J Theunissen, Danyel G J Jennen, Theo M C M de Kok, Jos C S Kleinjans
Valproic acid (VPA) is a very potent anti-cancer and neuro-protective drug probably by its HCAC inhibiting properties, which may cause steatosis in the liver. The present study investigates the effect of repetitive VPA treatment of primary human hepatocytes (PHH) on whole genome gene expression-, DNA methylation-, and miRNA changes, using microarrays and integrated data analyses. PHH were exposed to a non-cytotoxic dose of VPA for 5days daily which induced lipid accumulation. Part of the PHH was left untreated for 3days for studying the persistence of 'omics' changes...
November 14, 2017: Toxicology
https://www.readbyqxmd.com/read/29154787/functionalization-of-paramagnetic-nanoparticles-for-protein-immobilization-and-purification
#9
Lara A B C Carneiro, Richard J Ward
A paramagnetic nanocomposite coated with chitosan and N-(5-Amino-1-carboxy-pentyl) iminodiacetic acid (NTA) that is suitable for protein immobilization applications has been prepared and characterized. The nanoparticle core was synthesized by controlled aggregation of Fe3O4 under alkaline conditions, and Transmission Electron Microscopy revealed a size distribution of 10-50 nm. The nanoparticle core was coated with chitosan and derivatized with glutaraldehyde and NTA, as confirmed by Fourier Transform Infrared Spectroscopy...
November 14, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29153581/novel-application-of-micro-computerized-tomography-for-morphologic-characterization-of-the-murine-penis
#10
Marisol O'Neill, Gene O Huang, Dolores J Lamb
BACKGROUND: The murine penis model has enriched our understanding of anomalous penile development. The morphologic characterization of the murine penis using conventional serial sectioning methods is labor intensive and prone to errors. AIM: To develop a novel application of micro-computerized tomography (micro-CT) with iodine staining for rapid, non-destructive morphologic study of murine penis structure. METHODS: Penises were dissected from 10 adult wild-type mice and imaged using micro-CT with iodine staining...
November 16, 2017: Journal of Sexual Medicine
https://www.readbyqxmd.com/read/29153515/daily-and-seasonal-trends-of-electricity-and-water-use-on-pasture-based-automatic-milking-dairy-farms
#11
J Shortall, B O'Brien, R D Sleator, J Upton
The objective of this study was to identify the major electricity and water-consuming components of a pasture-based automatic milking (AM) system and to establish the daily and seasonal consumption trends. Electricity and water meters were installed on 7 seasonal calving pasture-based AM farms across Ireland. Electricity-consuming processes and equipment that were metered for consumption included milk cooling components, air compressors, AM unit(s), auxiliary water heaters, water pumps, lights, sockets, automatic manure scrapers, and so on...
November 15, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29152241/effects-of-matrix-on-plasma-levels-of-epa-and-dha-in-dogs
#12
Kay Goffin, Marc van Maris, Ronald J Corbee
EPA and DHA are often used in veterinary medicine due to their beneficial effects for several medical conditions such as osteoarthritis. EPA and DHA are administered to dogs through different matrices. The aim of the present study was to determine the effects on the plasma levels in dogs caused by various matrices for EPA and DHA administration. In this study, three different n-3 PUFA formulations were used: soft chew tablet (CCx); liquid fish oil (LFO); and enriched kibbles (EK). The formulations were administered single-dose and compared in a randomised, cross-over designed study with a 1-week wash-out period...
2017: Journal of Nutritional Science
https://www.readbyqxmd.com/read/29152072/platelet-derived-tgf-%C3%AE-promotes-cervical-carcinoma-cell-growth-by-suppressing-klf6-expression
#13
Ao-Di He, Shao-Ping Wang, Wen Xie, Wei Song, Shuo Miao, Ru-Ping Yang, Ying Zhu, Ji-Zhou Xiang, Zhang-Yin Ming
Platelets in the primary tumor microenvironment play crucial roles in regulating tumor growth, metastasis, and angiogenesis, but the underlying mechanisms are unclear. Here, we show that platelet releasates exhibited a proliferative effect on HeLa cells, and this effect correlated with a reduction of KLF6 expression. After incubation with either washed human platelets or collagen-related peptide (CRP) activated platelet releasates, expression of KLF6 in the HeLa cervical tumor cell line was markedly reduced...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29151936/multiple-guided-technologies-based-on-radial-probe-endobronchial-ultrasound-for-the-diagnosis-of-solitary-peripheral-pulmonary-lesions-a-single-center-study
#14
Huang Haidong, Ning Yunye, Zhang Wei, Paul Zarogoulidis, Wolfgang Hohenforst-Schmidt, Yan-Gao Man, Yang Yuguang, Dong Yuchao, Bai Chong
Objective To evaluate the value of multiple guided technologies based on radial probe endobronchial ultrasound (R-EBUS) in the application of the diagnosis of solitary pulmonary peripheral lesions (PPLs). Methods Analysis of diagnostic yield in 4 groups patients with different combined multiple guided technologies based on R-EBUS, 94 patients with 94 solitary PPLs from Mar, 2013 to Nov, 2014 in Changhai Hospital. Results The overall diagnostic yield was 75% (70/94), the diagnostic yield of Group A (R-EBUS) was 62%(16/26), Group B (R-EBUS with guided sheath, EBUS-GS) was 76% (34/45), Group C (EBUS-GS with fluoroscopy) was 82% (9/11), Group D (virtual bronchoscopic navigation guided EBUS-GS with fluoroscopy) was 92% (11/12)...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/29150736/effect-of-erythrocyte-sperm-separation-medium-on-nuclear-acrosomal-and-membrane-maturity-parameters-in-human-sperm
#15
Bikem Soygur, Soner Celik, Ciler Celik-Ozenci, Leyla Sati
PURPOSE: The purpose of this study is to investigate whether erythrocyte-sperm separation medium (ESSM) has effects on human sperm motility, morphology, viability, membrane maturity, acrosome integrity, and nuclear attributes before and after cryopreservation. METHODS: Semen samples from normozoospermic (n = 36) and oligozoospermic (n = 9) patients were analyzed. Samples from the same patient were divided into three aliquots: group 1 and group 2 were resuspended in sperm washing media and ESSM, respectively...
November 18, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29150255/effects-of-red-earthworms-eisenia-fetida-on-leachability-of-lead-minerals-in-soil
#16
Armin Kavehei, Grant C Hose, Damian B Gore
Contamination of soils by metals and metalloids is an important environmental problem in many residential and industrial sites around the world. Lead is a common contaminant, which enters the soil through mining, industrial activities and waste disposal. A range of technologies can be used to remediate soil lead, however most remediation technologies adversely affect the environment and particularly soil biota. We have assessed the efficacy of vermiremediation (the use of earthworms for remediation) to reduce water extractable lead concentrations in soil...
November 14, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29149905/the-kusamala-program-for-primary-caregivers-of-children-6-59-months-of-age-hospitalized-with-severe-acute-malnutrition-in-malawi-study-protocol-for-a-cluster-randomized-controlled-trial
#17
Allison I Daniel, Meta van den Heuvel, Wieger P Voskuijl, Melissa Gladstone, Mike Bwanali, Isabel Potani, Celine Bourdon, Jenala Njirammadzi, Robert H J Bandsma
BACKGROUND: Severe acute malnutrition (SAM) is associated with high mortality rates and impairments in growth and development in children that do survive. There are complex nutritional, health, and behavioural risk factors involving severely malnourished children and their primary caregivers, requiring integrated intervention approaches. METHODS: A cluster-randomized controlled trial at the Queen Elizabeth Central Hospital in Blantyre, Malawi will be conducted to evaluate the effectiveness of a 4-day hospital-based intervention programme directed at primary caregivers...
November 17, 2017: Trials
https://www.readbyqxmd.com/read/29149742/stability-of-heavy-metals-in-soil-washing-residue-with-and-without-biochar-addition-under-accelerated-ageing
#18
Zhengtao Shen, Deyi Hou, Bin Zhao, Wendi Xu, Yong Sik Ok, Nanthi S Bolan, Daniel S Alessi
Soil washing residue (SWR), which typically concentrates the washed toxic metals and is comprised of high contents of clay particles, may pose risks to the surrounding environment. This study aims to simulate accelerated ageing to assess the stability of selected metals (Cd(2+) (132mg/kg), Cu(2+) (248mg/kg) and Pb(2+) (3470mg/kg)) in a SWR (89.68% of clay) with and without biochar treatment. The soil was incubated under constant moisture and wet-dry cycles (accelerated ageing), respectively, and the mobility and fractions of heavy metals in the soils with and without biochar treatment were examined...
November 14, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29148876/food-safety-at-farmers-markets-a-knowledge-synthesis-of-published-research
#19
Ian Young, Abhinand Thaivalappil, Danielle Reimer, Judy Greig
Farmers' markets are increasingly popular venues in North America for the sale of fresh produce and other foods. However, the nature of their operation can present possible food safety issues, challenges, and risks to consumers. A knowledge synthesis was conducted to identify, characterize, and summarize published research on the microbial food safety issues and implications associated with farmers' markets. A scoping review was conducted using the following steps: comprehensive search strategy, relevance screening of abstracts, and characterization of relevant articles...
November 17, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/29148650/residual-effectiveness-of-permethrin-treated-clothing-for-prevention-of-mosquito-bites-under-simulated-conditions
#20
Stephanie L Richards, Jo Anne G Balanay, Jonathan W Harris, Victoria M Banks, Steven Meshnick
Biological hazards such as exposure to ticks and mosquitoes can affect health. Permethrin-treated clothing is available to the public. We don’t currently understand, however, the effects of environmental factors such as fabric type, washing, sunlight, and temperature on permethrin content in treated clothing with respect to mosquito knockdown and mortality. We evaluated the extent to which fabric type (100% cotton denim jeans, 100% polyester work shirt, 35% cotton/65% polyester work shirt), light exposure (0 or 100%), temperature (18 °C, 32 °C), and number of washes (0, 3, 12, 36) affected mosquito knockdown 2 hours post-exposure, mosquito mortality 24 hours post-exposure, and permethrin content...
April 2017: Journal of Environmental Health
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