Xiaofeng Zuo, Brennan Winkler, Kasey Lerner, Daria V Ilatovskaya, Aleksandra S Zamaro, Yujing Dang, Yanhui Su, Peifeng Deng, Wayne Fitzgibbon, Jessica Hartman, Kwon Moo Park, Joshua H Lipschutz
The exocyst and Ift88 are necessary for primary ciliogenesis. Overexpression of Exoc5 (OE), a central exocyst component, resulted in longer cilia and enhanced injury recovery. Mitochondria are involved in acute kidney injury (AKI). To investigate cilia and mitochondria, basal respiration and mitochondrial maximal and spare respiratory capacity were measured in: Exoc5 OE, Exoc5 knockdown (KD), Exoc5 ciliary targeting sequence mutant (CTS-mut), control MDCK, Ift88 knockout (KO), and Ift88 rescue cells. In Exoc5 KD, Exoc5 CTS-mut, and Ift88 KO cells, these parameters were decreased...
May 9, 2024: American Journal of Physiology. Renal Physiology