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Amniotic membrane graft

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https://www.readbyqxmd.com/read/27888184/amnion-assisted-conjunctival-epithelial-redirection-in-limbal-stem-cell-grafting
#1
Harminder S Dua, Ammar Miri, Mohamed S Elalfy, Anna Lencova, Dalia G Said
AIMS: To develop a technique using amniotic membrane (AM) to prevent admixture of conjunctival epithelial cells and limbal explant-derived corneal epithelial cells in patients undergoing limbal stem cell transplantation. To compare this technique with the current method of 'sequential sector conjunctival epitheliectomy' (SSCE). METHODS: 26 patients with total limbal stem cell deficiency who underwent ocular surface reconstruction with limbal stem cells transplantation were retrospectively studied...
November 25, 2016: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/27843896/surgical-reconstruction-of-ocular-surface-tumors-using-fibrin-sealant-tissue-adhesive
#2
Aline Roseane Queiroz de Paiva, Larissa Abreu de Azevedo Fraga, Virgínia Laura Lucas Torres
PURPOSE: To evaluate the surgical outcomes of ocular surface reconstruction in corneal-conjunctival tumors using fibrin tissue adhesive. METHODS: A prospective noncomparative study was performed between May 2013 and February 2015. Patients were submitted to routine surgical procedure for corneal-conjunctival tumor excision followed by amniotic membrane graft transplantation using fibrin tissue adhesive (Evicel®, Omrix Biopharmaceuticals Ltd., Israel). Results were assessed on the 1st, 7th, 15th and 30th postoperative days to analyze subjective complaints, adhesiveness and positioning of the graft, potential complications and recurrences...
October 2016: Ocular Oncology and Pathology
https://www.readbyqxmd.com/read/27830086/active-pedicle-epithelial-flap-transposition-combined-with-amniotic-membrane-transplantation-for-treatment-of-nonhealing-corneal-ulcers
#3
Ting Zhang, Yuexin Wang, Yanni Jia, Dongle Liu, Suxia Li, Weiyun Shi, Hua Gao
Introduction. The objective was to evaluate the efficacy of active pedicle epithelial flap transposition combined with amniotic membrane transplantation (AMT) in treating nonhealing corneal ulcers. Material and Methods. Eleven patients (11 eyes) with nonhealing corneal ulcer who underwent the combined surgery were included. Postoperatively, ulcer healing time was detected by corneal fluorescein staining. Visual acuity, intraocular pressure, surgical complications, and recurrence were recorded. Corneal status was inspected by the laser scanning confocal microscopy and anterior segment optical coherence tomography (AS-OCT)...
2016: Journal of Ophthalmology
https://www.readbyqxmd.com/read/27788828/decellularized-amniotic-membrane-scaffold-as-a-pericardial-substitute-an-in%C3%A2-vivo-study
#4
J C Francisco, R Correa Cunha, M A Cardoso, R Baggio Simeoni, B F Mogharbel, G L Picharski, D Silva Moreira Dziedzic, L C Guarita-Souza, K A T Carvalho
BACKGROUND: In the development of new biomaterials for pericardium substitute, acellular amniotic membrane (AAM) presents potential for new applications in regenerative medicine. We studied an AAM as a pericardial substitute to achieve a suitable, cost effective, abundant matrix for the purpose of using it as graft for tissue repair. METHODS: Twenty Wistar rats were randomly divided into 2 groups (n = 10/group) and had their pericardiums excised. In the experimental group, the excised pericardium segment was substituted by a 7-mm-diameter patch of decellularized AAM sutured to the lesion area...
October 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27761704/limbal-conjunctival-versus-amniotic-membrane-in-the-intraoperative-application-of-mitomycin-c-for-recurrent-pterygium-a-randomized-controlled-trial
#5
Rongxin Chen, Guofu Huang, Shu Liu, Wenfang Ma, Xiaofang Yin, Shiyou Zhou
PURPOSE: This study compared the outcomes of a limbal conjunctival autograft (LCAG) with those of an amniotic membrane graft (AMG) followed by intraoperative 0.02 % mitomycin C (MMC) to treat recurrent pterygium. METHODS: In this randomized controlled trial, ninety-six eyes with recurrent pterygium were enrolled and randomly allocated into two groups using a computer-generated random number table. Pterygium removal was followed by intraoperative 0.02 % MMC for 3 min and then either LCAG or AMG transplantation...
October 20, 2016: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/27759861/dehydrated-allogenic-human-amniotic-membrane-graft-for-conjunctival-surface-reconstruction-following-removal-of-exposed-scleral-buckle
#6
Dilraj S Grewal, Tamer H Mahmoud
A 54-year-old male developed symptomatic scleral buckle exposure 16 years after placement and presented with large conjunctival defects with fibrosed edges. Following surgical removal of the buckle elements, primary conjunctival closure could not be achieved due to significant wound tension despite undermining the conjunctival edges to mobilize the conjunctiva. A dehydrated amniotic membrane graft (Ambio5; IOP Ophthalmics, Costa Mesa, CA) was placed on the scleral bed and secured in place with fibrin sealant (TISSEEL; Baxter International, Westlake Village, CA)...
October 1, 2016: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/27717107/management-of-patients-with-ocular-manifestations-in-vesiculobullous-disorders-affecting-the-mouth
#7
REVIEW
M S Hansen, O N Klefter, H O Julian, A M Lynge Pedersen, S Heegaard
Pemphigoid and pemphigus diseases as well as Stevens-Johnson syndrome present as vesiculobullous disorders of the skin and may additionally involve both the oral cavity and the ocular surface. Ocular involvement ranges from mild irritation and dry eye disease to chronic conjunctivitis, symblepharon, eyelid malposition, ocular surface scarring and severe visual loss. In addition to diagnostic assessments, ophthalmologists must treat the dry eye and meibomian gland dysfunction components of these diseases using a stepladder approach, including eyelid hygiene and lubricants...
October 7, 2016: Oral Diseases
https://www.readbyqxmd.com/read/27651882/clinical-comparison-of-coronally-advanced-flap-plus-amniotic-membrane-or-subepithelial-connective-tissue-in-the-treatment-of-miller-s-class-i-and-ii-gingival-recessions-a-split-mouth-study
#8
Ardeshir Lafzi, Nader Abolfazli, Masoumeh Faramarzi, Masoumeh Eyvazi, Amir Eskandari, Fariba Salehsaber
Background. The aim of the present study was to compare coronally advanced flap (CAF) plus amniotic membrane (AM) to CAF with connective tissue graft (CTG) in the treatment of Miller's class I and II gingival recessions. Methods. Eleven healthy subjects with thirty Miller's class І and ІІ gingival recessions ≥3 mm, were selevted for this research and randomly assigned to two groups in a split-mouth design. In the control group gingival recessions were treated with CAF and CTG; however, in the test group the lesions were treated with (AM) and CAF...
2016: Journal of Dental Research, Dental Clinics, Dental Prospects
https://www.readbyqxmd.com/read/27649406/comparing-the-efficacy-of-viable-cryopreserved-placental-membrane-for-the-treatment-of-wounds-of-diverse-etiologies
#9
Derek S Anselmo, Marc Karpo, Ebony Love, Tracey Vlahovic
INTRODUCTION AND OBJECTIVES: The benefits of using amniotic tissue in skin regeneration are well documented. Today, cryopreservation technology allows for better availability and maintenance of mesenchymal stem cells. This is of particular interest in treating the diabetic foot ulcer as this population has fewer mesenchymal stem cells. The objective of this case series investigation was to compare the efficacy of cryopreserved human amniotic stem cells in treating foot wounds of different etiologies...
September 2016: Journal of the American Podiatric Medical Association
https://www.readbyqxmd.com/read/27565784/long-term-follow-up-of-transplantation-of-preserved-limbal-allograft-and-amniotic-membrane-for-recurrent-pterygium
#10
Takashi Ono, Yosai Mori, Ryohei Nejima, Tadatoshi Tokunaga, Kazunori Miyata, Shiro Amano
PURPOSE: To conduct a long-term follow-up study evaluating the efficacy and safety of transplantation of preserved limbal allograft and amniotic membrane for recurrent pterygium. METHODS: This was a retrospective, non-comparative, interventional case series conducted at a private eye hospital. Eighty-four eyes of 80 patients with recurrent pterygium were included in the study. The mean number of previous surgeries for pterygium was 1.36 ± 0.98 (range, 1-8)...
December 2016: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/27520087/role-of-artificial-tears-in-reducing-the-recurrence-of-pterygium-after-surgery-a-prospective-randomized-controlled-trial
#11
Kosol Kampitak, Wichai Leelawongtawun, Supinda Leeamornsiri, Wannisa Suphachearaphan
PURPOSE: To determine whether artificial tears can reduce recurrence of pterygium after surgery. METHODS: A total of 128 primary pterygium cases after excision were randomized into two groups according to postoperative drugs: 64 cases received topical dexamethasone (control group), and 64 cases received topical dexamethasone and hydroxypropyl methylcellulose (treatment group). The pterygium was removed with amniotic membrane graft technique in all cases. Recurrence was observed within 6 months after surgery, and survival analysis was used to evaluate the difference between groups...
August 13, 2016: Acta Ophthalmologica
https://www.readbyqxmd.com/read/27507558/long-term-result-of-autologous-cultivated-oral-mucosal-epithelial-transplantation-for-severe-ocular-surface-disease
#12
Pinnita Prabhasawat, Pattama Ekpo, Mongkol Uiprasertkul, Suksri Chotikavanich, Nattaporn Tesavibul, Kanograt Pornpanich, Panitee Luemsamran
The present study aimed to investigate the clinical outcomes of autologous cultivated oral mucosal epithelial transplantation (COMET) on human amniotic membrane (AM) for corneal limbal stem cell deficiency (LSCD). In this prospective, noncomparative case series, 20 eyes (18 patients) with bilateral severe ocular surface disease were chosen to undergo COMET on human AM. The primary outcome was clinical success, and the secondary outcomes were the best-corrected visual acuity difference, corneal opacification, symblepharon formation, and complications...
September 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27453750/new-clinical-application-of-amniotic-membrane-transplant-for-ocular-surface-disease
#13
A Gheorghe, M Pop, M Burcea, M Serban
UNLABELLED: The new defined anatomical and functional complex conjunctiva-limbus-cornea is a new concept, which helps clinicians better understand and treat ocular surface pathologies. The management of the ocular surface disease has changed dramatically over the years, with spectacular improvements of techniques, and of course, results. The amniotic membrane, used as a graft or as a substrate for the cultivation of limbal corneal cells has showed encouraging results. AIM: To investigate the usefulness of amniotic membrane transplantation in ocular surface pathologies...
April 2016: Journal of Medicine and Life
https://www.readbyqxmd.com/read/27450147/management-of-severe-and-refractory-mooren-s-ulcers-with-rituximab
#14
Damien Guindolet, Clotilde Reynaud, Gaelle Clavel, Georges Belangé, Maycene Benmahmed, Serge Doan, Gilles Hayem, Isabelle Cochereau, Eric E Gabison
PURPOSE: Management of severe and refractory Mooren's ulcers is challenging as it encompasses tectonic surgical treatment and aggressive immunosuppressive therapies. Efficacy of rituximab in the management of severe Mooren's ulcers has never been reported. METHODS: Five patients (six eyes) from the Cornea and External Disorders department at the Rothschild Ophthalmologic Foundation (Paris, France) were treated for severe Mooren's ulcer unresponsive to conventional treatments between 2008 and 2016...
July 22, 2016: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/27430235/optimizing-amniotic-membrane-tissue-banking-protocols-for-ophthalmic-use
#15
D Hettiarachchi, V H W Dissanayake, H W W Goonasekera
Amniotic membrane (AM) due to its anti-inflammatory, anti-scarring and anti-angiogenic properties is used as corneal and wound grafts. When developing AM tissue banks, cell viability, membrane morphology and genomic stability should be preserved following cryopreservation. To analyze the changes rendered to the AM during the process of cryopreservation by comparing different combinations of standard cryopreservation media; fetal bovine serum (FBS), dimethyl sulfoxide (DMSO), Dulbecco's modified eagle's medium (DMEM) and glycerol at -80 °C and at -196 °C for a period of 6 weeks and at 4 °C in 70 % alcohol for 6 weeks...
September 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27413501/fornix-reconstruction-with-amniotic-membrane-transplantation-a-cosmetic-remedy-for-blind-patients
#16
Shreya Thatte, Jagriti Jain
PURPOSE: Fornix contracture is an obstacle for fitting a prosthesis in blind or enucleated patients, and may lead to disfigurement and psychological issues. This study evaluates the efficacy of amniotic membrane transplantation (AMT) for fornix reconstruction with the aim of better retention of the ocular prosthesis. METHODS: This non-comparative interventional case series includes eighteen blind eyes with fornix deformity in which a cosmetic prosthesis could not be retained...
April 2016: Journal of Ophthalmic & Vision Research
https://www.readbyqxmd.com/read/27405284/amniotic-membrane-transplantation-with-narrow-strip-conjunctival-autograft-vs-conjunctival-autograft-for-recurrent-pterygia
#17
José Bonifácio Barbosa, Charles Costa De Farias, Flávio Eduardo Hirai, José Álvaro Pereira Gomes
PURPOSE: To compare amniotic membrane transplantation (AMT) associated with narrow-strip conjunctival autograft vs conjunctival autograft alone for the treatment of recurrent pterygium. METHODS: In this prospective consecutive interventional study, patients with recurrent pterygium were randomly divided into one of 2 groups; group 1: patients undergoing AMT associated with autologous conjunctival graft; and group 2: patients undergoing conjunctival autograft alone...
July 12, 2016: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/27390533/amniotic-membrane-can-be-a-valid-source-for-wound-healing
#18
Hossam ElHeneidy, Eman Omran, Ahmed Halwagy, Hesham Al-Inany, Mirvat Al-Ansary, Amr Gad
UNLABELLED: Amniotic membrane (AM) can promote proper epithelialization with suppression of excessive fibrosis by creating a supportive milieu for regeneration of chronic ulcer bed. OBJECTIVE: The objective of this study is to investigate whether AM scaffold can modulate the healing of a wound by promoting tissue reconstruction rather than promoting scar tissue formation. SUBJECTS AND METHODS: AM was obtained and prepared and then applied to patients with chronic leg ulcers who were randomly divided into two different groups...
2016: International Journal of Women's Health
https://www.readbyqxmd.com/read/27362882/existence-of-normal-limbal-epithelium-in-eyes-with-clinical-signs-of-total-limbal-stem-cell-deficiency
#19
Eric Chan, Qihua Le, Andres Codriansky, Jiaxu Hong, Jianjian Xu, Sophie X Deng
PURPOSE: To report the presence of normal limbal epithelium detected by in vivo confocal laser scanning microscopy (IVCM) in 3 cases of clinically diagnosed total limbal stem cell deficiency (LSCD). METHODS: This is a retrospective case report consisting of 3 patients who were diagnosed with total LSCD based on clinical examination and/or impression cytology. Clinical data including ocular history, presentation, slit-lamp examination, IVCM, and impression cytology were reviewed...
November 2016: Cornea
https://www.readbyqxmd.com/read/27308239/the-healing-effect-of-amniotic-membrane-in-burn-patients
#20
Mahdi Eskandarlou, Mina Azimi, Soghra Rabiee, Mohammad Ali Seif Rabiee
BACKGROUND: Different methods for dressing of donor site of skin graft in burn patients have similarly pain, limitation of mobility of donor site and local complications such as infection and scar. Amniotic membrane has used for improvement of healing in some wounds. Accordingly in this study amnion was used as biologic dressing for donor site of skin graft to evaluate it's efficacy in improvement of pain, move score and the risk of local infection. METHODS: Study was done as clinical trial over 32 admitted patients in burn department of Beasat hospital...
January 2016: World Journal of Plastic Surgery
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