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Amniotic membrane graft

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https://www.readbyqxmd.com/read/28912992/giant-conjunctival-nevus-in-a-12-year-old-child
#1
Edit Tóth-Molnár, Eszter Vizvári, Ákos Skribek, András Vörös
We describe a case of a giant conjunctival nevus presented in a 12-year-old girl with suspicious clinicomorphological appearance. The lesion was noticed by the parents at the age of 3 years as a "fleshy spot" on the bulbar conjunctiva. The lesion remained unchanged until approx. 6 months before recent admission. On slit-lamp examination, a large conjunctival lesion with variegate pigmentation and indistinct margins was detected on the superonasal part of the bulbar conjunctiva of the left eye. Intralesional cysts and vessels were detected with AS-OCT examination...
2017: Case Reports in Ophthalmological Medicine
https://www.readbyqxmd.com/read/28893657/the-effect-of-adjuvant-treatment-to-prevent-and-treat-intrauterine-adhesions-a-network-meta-analysis-of-randomized-controlled-trials
#2
REVIEW
Yu Yan, Dongmei Xu
Intrauterine adhesions (IUA) can lead to partial or complete closure of the uterine cavity, which may result in symptoms including abnormal menstruation, infertility, and pelvic pain. A network meta-analysis was performed to assess the effect of adjuvant therapy on the prevention and treatment of intrauterine adhesions. We searched electronic databases, including PubMed, EmBase, and the Cochrane Library, up to May 5, 2017 without language restrictions. The primary outcomes in the present analysis were the rate of IUAs for prevention and the rate of IUA recurrence for treatment...
September 8, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28835428/oral-mucosal-grafting-combined-with-tenonplasty-for-ocular-surface-and-lid-margin-reconstruction-in-an-atypical-sectorial-chemical-burn
#3
Swati Singh, Purvasha Narang, Vikas Mittal
A 46-year-old woman presented with chemical injury in both eyes after the instillation of undefined eye-drops prescribed by quack. She had an atypical presentation in the form of bilateral severe necrosis of both lids and whole palpebral conjunctiva. Extensive debridement with conjunctival epitheliectomy, tenonplasty and amniotic membrane transplantation (AMT) was performed. Characteristic differences from typical chemical injuries were a sectorial involvement of ocular surface and keratinisation over distorted lid margins...
August 23, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28835072/-advances-in-preparation-and-clinical-application-of-amniotic-membrane-graft
#4
S Q Zheng, T S Chen, S Z Ji, P F Luo, S C Xiao
Amniotic membrane has been used as wound dressing for more than 100 years. With the development of the preservation and preparation techniques, amniotic membrane is widely used in ophthalmology, burns, plastic surgery, dentistry, and neurosurgery. In recent years, as more and more amniotic membrane is used in chronic wounds, it will be a new treatment method for wounds. This paper is a brief review about advances in preparation and clinical application of amniotic membrane graft.
August 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28806949/case-report-of-ocular-kaposi-s-sarcoma
#5
Jing Yang, Xiao-Fang Yin, Yong-Ping Li, Shi-You Zhou
BACKGROUND: Kaposi's sarcoma (KS) is generally considered a neoplastic disorder of vascular origin and occurs in patients with acquired immunodeficiency syndrome (AIDS) or who have received immunosuppressive treatments after an organ transplant (Soulier et al., Blood 86(4):1276-80, 1995; Viejo-Borbolla and Schulz, AIDS Rev 5(4):222-9, 2003; Schulz, J Antimicrob Chemother 45(Suppl T3):15-27, 2000; Aversa et al. Crit Rev Oncol Hematol 53(3):253-65, 2005; Mbulaiteye and Engels, Int J Cancer 119(11):2685-91, 2006; Tessari et al...
August 14, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28770884/outcome-of-application-of-amniotic-membrane-graft-in-ocular-surface-disorders
#6
Nargis Nizam Ashraf, Muhammad Idrees Adhi
OBJECTIVE: To determine the outcome of application of amniotic graft in ocular surface disorders. METHODS: This cross-sectional study was conducted at Dow University of Health Sciences, Karachi, from January 2010 to December 2012, and comprised patients with ocular surface disorders. Patients' presenting symptoms and signs were recorded. Previously harvested and frozen amniotic graft was applied in different types of ocular surface disorders, such as corneal ulcers, pterygium, keratomalacia, Steven-Johnson syndrome, etc...
July 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28742619/use-of-topical-insulin-to-treat-refractory-neurotrophic-corneal-ulcers
#7
Angeline L Wang, Eric Weinlander, Brandon M Metcalf, Neal P Barney, David M Gamm, Sarah M Nehls, Michael C Struck
PURPOSE: To report the clinical course of 6 patients with refractory neurotrophic corneal ulcers that were treated with topical insulin drops. METHODS: Retrospective chart review of patients who had neurotrophic corneal ulcers or epithelial defects refractory to standard medical and surgical treatment. Insulin drops, prepared by mixing regular insulin in artificial tears with a polyethylene glycol and propylene glycol base at a concentration of 1 unit per milliliter, were prescribed 2 to 3 times daily...
July 24, 2017: Cornea
https://www.readbyqxmd.com/read/28730219/histological-immunohistochemical-and-clinical-considerations-on-amniotic-membrane-transplant-for-ocular-surface-reconstruction
#8
Elena Şapte, Claudia Florida Costea, Alexandru Cărăuleanu, Cristina Dancă, Gabriela FlorenŢa Dumitrescu, Gabriela Dimitriu, Mădălina Adriana Chihaia, Cătălin Mihai Buzdugă, Andrei Cucu, Mihaela Dana Turliuc
Amniotic membrane (AM) transplantation has been used successfully worldwide in ophthalmology plastic surgery for over 100 years. This review presents the histological and the immunohistochemical features of AM compared to those of the conjunctiva and discusses the techniques of processing and preservation, its mechanism of action in ocular reconstruction, its clinical ophthalmic indications, but also advantages and limitations of grafting with this biomaterial.
2017: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
https://www.readbyqxmd.com/read/28715585/retinoic-acid-engineered-amniotic-membrane-used-as-graft-or-homogenate-positive-effects-on-corneal-alkali-burns
#9
Romain Joubert, Estelle Daniel, Nicolas Bonnin, Aurélie Comptour, Christelle Gross, Corinne Belville, Frédéric Chiambaretta, Loïc Blanchon, Vincent Sapin
Purpose: Alkali burns are the most common, severe chemical ocular injuries, their functional prognosis depending on corneal wound healing efficiency. The purpose of our study was to compare the benefits of amniotic membrane (AM) grafts and homogenates for wound healing in the presence or absence of previous all-trans retinoic acid (atRA) treatment. Methods: Fifty male CD1 mice with reproducible corneal chemical burn were divided into five groups, as follows: group 1 was treated with saline solution; groups 2 and 3 received untreated AM grafts or grafts treated with atRA, respectively; and groups 4 and 5 received untreated AM homogenates or homogenates treated with atRA, respectively...
July 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28700252/modified-allogenic-simple-limbal-epithelial-transplantation-followed-by-keratoplasty-as-treatment-for-total-limbal-stem-cell-deficiency
#10
Alfonso Vasquez-Perez, Mayank A Nanavaty
PURPOSE: To describe a modified allogenic simple limbal epithelial transplant (SLET) technique with large donor tissue explants followed by keratoplasty. METHODS: A 69-year-old with conjunctival melanoma on her left eye developed total limbal stem cell deficiency (LSCD) after multiple cycles of topical mitomycin. She also had herpes stromal keratitis while on treatment with mitomycin. She underwent modified allogenic SLET with large donor limbal tissue explants glued on the cornea directly and the amniotic membrane placed over the limbal explants...
July 12, 2017: Ocular Immunology and Inflammation
https://www.readbyqxmd.com/read/28673520/similarities-between-induced-membrane-and-amniotic-membrane-novelty-for-bone-repair
#11
Florelle Gindraux, Thomas Rondot, Benoit de Billy, Narcisse Zwetyenga, Jean-Christophe Fricain, Aurélie Pagnon, Laurent Obert
Previous clinical studies have shown the efficacy of a two-stage surgical procedure - the induced membrane (IM) technique - for reconstruction of large bone defects or bone non-union. The first stage involves radical debridement and insertion of a cement spacer into the bone defect. The second stage, performed weeks to months later, consists of removing the spacer while leaving the foreign body membrane induced by the cement in place, and then filling the cavity with bone autograft. The IM has been shown to (1) act as a protective physical barrier by preventing bone autograft resorption and (2) act as a bioreactor by promoting healing through revascularisation and growth factor secretion, and by concentrating mesenchymal stem cells (MSC) with osteogenic properties...
June 23, 2017: Placenta
https://www.readbyqxmd.com/read/28586928/-biomaterials-or-donor-tissue-what-is-the-future-of-tissue-engenieering-for-cornea-reconstruction
#12
Björn O Bachmann, Stefan Schrader
For the replacement of corneal tissue, corneal grafts or amniotic membrane are still used as a standard material. Since this is biological tissue, there is only a limited standardization regarding preparation, tissue properties and behaviour after transplantation. In addition, there is a risk of disease transmission, and the availability of both human corneas and amniotic membrane is insufficient in many regions of the world, which is why alternative biomaterials have been explored for many years now. Among the natural biomaterials, materials based on collagen or keratin provide characteristics that make them good candidates for corneal tissue replacement...
June 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/28557902/cryopreserved-amniotic-membrane-improves-clinical-outcomes-following-microdiscectomy
#13
D Greg Anderson, Victor Popov, Andrew L Raines, Julie O'Connell
STUDY DESIGN: Prospective, randomized controlled trial. OBJECTIVE: To compare pain, physical/mental functional recovery and recurrent herniation for patients following lumbar microdiscectomy with and without the use of a cryopreserved amniotic tissue graft. SUMMARY OF BACKGROUND DATA: Although microdiscectomy procedures are routinely successful for patients with lumbar radiculopathy due to herniated disc disease, residual low back pain, and recurrent herniation remain unsolved clinical problems...
May 26, 2017: Clinical Spine Surgery
https://www.readbyqxmd.com/read/28553162/bladder-reconstruction-with-human-amniotic-membrane-in-a-xenograft-rat-model-a-preclinical-study
#14
Dimitri Barski, Holger Gerullis, Thorsten Ecke, Jin Yang, Gabriella Varga, Mihaly Boros, Isabel Pintelon, Jean-Pierre Timmermans, Thomas Otto
Background: Human amniotic membranes (HAMs) are assumed to have a number of unique characteristics including durability, hypoallergenic and anti-inflammatory properties. Materials and Methods: Multilayer HAMs from caesarian sections were applied to repair defined bladder defects in male Sprague-Dawley rats. The animals were sacrificed at 7, 21 and 42 days after implantation. Bladder volume capacity after grafting was measured. Histological analyses were performed to asses a number of parameters including HAM degradation, inflammatory reaction, graft rejection and smooth muscle ingrowth...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28550448/towards-xeno-free-cultures-of-human-limbal-stem-cells-for-ocular-surface-reconstruction
#15
Zala Lužnik, Claudia Breda, Vanessa Barbaro, Stefano Ferrari, Angelo Migliorati, Enzo Di Iorio, Barbara Ferrari, Carlo Griffoni, Andrea Grassetto, Hossein Mostafa Elbadawy, Marina Bertolin
Isolated limbal epithelial stem cells (LESCs) were cultured with or without a 3T3 murine fibroblast feeder-layer (FL) in 4 different culture media on culture plates or on denuded human amniotic membrane (AM) support and fibrin gel support: (1) control medium supplemented with fetal bovine serum; (2) control medium supplemented with the synthetic serum "XerumFree™ XF205" (XF); (3) CnT-20 medium supplemented with "XerumFree™ XF205" (CnT-XF) and (4) CnT-20 medium supplemented with human AB serum (CnT-AB). The three xenogeneic media were compared to standard condition (control + FL) and parameters assessed included cell morphology, proliferative potential, number of passages, assessment of clonogenic and abortive colonies, life span, ∆Np63α expression and epithelial morphology on AM...
May 26, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28548553/human-amniotic-membrane-is-not-suitable-for-the-grafting-of-colon-lesions-and-prevention-of-adhesions-in-a-xenograft-rat-model
#16
Dimitri Barski, Holger Gerullis, Thorsten Ecke, Gabriella Varga, Mihaly Boros, Isabel Pintelon, Jean-Pierre Timmermans, Thomas Otto
INTRODUCTION: New biological materials are needed for specific applications in reconstructive bowel surgery and for the prevention of adhesion formation. Amniotic membranes (AMs) are assumed to have a number of unique characteristics that enhance the ingrowth of the surrounding tissue. The aim of the present study was to provide proof of these qualities in a xenograft model. MATERIALS AND METHODS: A multilayer human AM (HAM) was applied to repair defined colon wall defects in Sprague-Dawley rats (n = 18)...
May 1, 2017: Surgical Innovation
https://www.readbyqxmd.com/read/28529415/using-amniotic-membrane-as-a-novel-method-to-reduce-post-burn-hypertrophic-scar-formation-a-prospective-follow-up-study
#17
Ali Akbar Mohammadi, Shima Eskandari, Hamed Ghoddusi Johari, Ata'ollah Rajabnejad
BACKGROUND: Several studies have shown that the application of amniotic membrane as a biological dressing in the management of burns is accompanied by rapid re-epithelialisation. In this follow-up study, we aimed to evaluate the possible role of amniotic membrane as an adjunct to split thickness skin grafting on reducing itching and severity of hypertrophic scar formation. MATERIALS AND METHODS: From October 2013 to January 2015, in a prospective follow-up study, 54 patients (108 limbs) with second and third degree burns, covering 4%-15% of total body surface area (TBSA), were included in the study...
January 2017: Journal of Cutaneous and Aesthetic Surgery
https://www.readbyqxmd.com/read/28512421/when-an-easy-thing-goes-wrong-foreign-body-induced-granuloma-associated-scleritis-following-pterygium-surgery
#18
Pedro Coelho, Carlos Menezes, Pedro Rodrigues, Rita Gonçalves, Tiago Maio, Jorge Moreira, Paula Tenedório
PURPOSE: To report a case of pterygium surgery with conjunctival autograft followed by focal necrotizing scleritis due to foreign body entrapment in the scleral bed. CASE REPORT/RESULTS: This is a case report of a 76-years-old male patient who underwent nasal pterygium surgery and developed focal necrotizing scleritis secondary to foreign body entrapment under conjunctival autograft. One month following surgery, slit-lamp examination demonstrated a progressive thinning of the surgical area with focal inflammatory signs...
January 2017: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/28370981/in-vitro-assessment-of-a-novel-hypothermically-stored-amniotic-membrane-for-use-in-a-chronic-wound-environment
#19
John P McQuilling, Jeremy B Vines, Katie C Mowry
Chronic wounds require extensive healing time and place patients at risk of infection and amputation. Recently, a fresh hypothermically stored amniotic membrane (HSAM) was developed and has subsequently shown promise in its ability to effectively heal chronic wounds. The purpose of this study is to investigate the mechanisms of action that contribute to wound-healing responses observed with HSAM. A proteomic analysis was conducted on HSAM, measuring 25 growth factors specific to wound healing within the grafts...
March 29, 2017: International Wound Journal
https://www.readbyqxmd.com/read/28344763/utility-of-human-amniotic-membrane-allograft-in-re-epithelialization-of-the-nasal-tip
#20
D'Antonio C Dennis, Adam R Turnock, Collin Sutton, Bradley Chastant, Wesley B Vanderlan
Variations in skin thickness and contours pose significant challenges to reconstruction of the lower third of the nose. Human amniotic membrane allograft offers a potential alternative to tissue transfer in reconstruction of the lower third of the nose. We reviewed the procedure and photographs of a healthy 56-year-old male with a 22 × 18 mm lower third nasal defect involving full thickness skin and subcutaneous tissue. Following preparation for grafting, dehydrated human amniotic membrane was fashioned to the dimensions of the defect and applied...
October 2016: Journal of Surgical Case Reports
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