Zigui Huang, Xiaoliang Huang, Yili Huang, Kunmei Liang, Lei Chen, Chuzhuo Zhong, Yingxin Chen, Chuanbin Chen, Zhen Wang, Fuhai He, Mingjian Qin, Chenyan Long, Binzhe Tang, Yongqi Huang, Yongzhi Wu, Xianwei Mo, Tang Weizhong, Jungang Liu
Gut microbiota has demonstrated an increasingly important role in the onset and development of colorectal cancer (CRC). Nonetheless, the association between gut microbiota and KRAS mutation in CRC remains enigmatic. We conducted 16S rRNA sequencing on stool samples from 94 CRC patients and employed the linear discriminant analysis effect size algorithm to identify distinct gut microbiota between KRAS mutant and KRAS wild-type CRC patients. Transcriptome sequencing data from nine CRC patients were transformed into a matrix of immune infiltrating cells, which was then utilized to explore KRAS mutation-associated biological functions, including Gene Ontology items and Kyoto Encyclopedia of Genes and Genomes pathways...
April 4, 2024: Microbiology Spectrum