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Ines Marek, Robert Becker, Fabian B Fahlbusch, Carlos Menendez-Castro, Wolfgang Rascher, Christoph Daniel, Gudrun Volkert, Andrea Hartner
BACKGROUND/AIMS: Healing of mesangioproliferative glomerulonephritis involves degradation of excess extracellular matrix, resolution of hypercellularity by apoptosis and phagocytosis of apoptotic cells. Integrin receptors participate in the regulation of phagocytosis. In mice deficient for alpha8 integrin (Itga8-/-) healing of glomerulonephritis is delayed. As Itga8 is abundant in mesangial cells (MC) which are non-professional phagocytes, we hypothesized that Itga8 facilitates phagocytosis of apoptotic cells and matrix components by MC...
March 10, 2018: Cellular Physiology and Biochemistry
Valeryi K Lishko, Valentin P Yakubenko, Tatiana P Ugarova, Nataly P Podolnikova
Platelet Factor 4 (PF4) is one of the most abundant cationic proteins secreted from α-granules of activated platelets. Based on its structure, PF4 was assigned to the CXC family of chemokines and has been shown to have numerous effects on myeloid leukocytes. However, the receptor for PF4 remains unknown. Here, we demonstrate that PF4 induces leukocyte responses through the integrin Mac-1 (αM β2 , CD11b/CD18). Human neutrophils, monocytes, U937 monocytic and HEK293 cells expressing Mac-1 strongly adhered to immobilized PF4 in a concentration-dependent manner...
March 14, 2018: Journal of Biological Chemistry
Sakihito Kitajima, Wataru Aoki, Daisuke Shibata, Daisuke Nakajima, Nozomu Sakurai, Kazufumi Yazaki, Ryosuke Munakata, Toki Taira, Masaru Kobayashi, Shunsuke Aburaya, Eric Hyrmeya Savadogo, Susumu Hibino, Haruna Yano
Latexes in immature fruit, young petioles and lignified trunks of fig trees protect the plant using toxic proteins and metabolites in various organ-dependent ways. Latexes from plants contain high amounts of toxic proteins and metabolites, which attack microbes and herbivores after exudation at pest-induced wound sites. The protein and metabolite constituents of latexes are highly variable, depending on the plant species and organ. To determine the diversity of latex-based defense strategies in fig tree (Ficus carica) organs, we conducted comparative proteomic, transcriptomic and metabolomic analyses on latexes isolated from immature fruit, young petioles and lignified trunks of F...
March 13, 2018: Planta
M Bedolla-Barajas, T R Bedolla-Pulido, M V Flores-Merino, A Jiménez-Rosales, M V Domínguez-García
Background. Oral allergy syndrome (OAS) is the most common food allergy manifestation amongst adults. However, population studies aimed at estimating its prevalence and associated factors are scarce in Mexi-co. Objectives. To establish the prevalence of OAS in a sample of university students and to describe their clinical characteristics and its associated factors. Methods. From a sample group made up of 25,269 univer-sity students, the data corresponding to 1,200 students aged 18 to 25 was analyzed with a cross-sectional approach...
February 28, 2018: European Annals of Allergy and Clinical Immunology
R Andler, S Hiessl, O Yücel, M Tesch, A Steinbüchel
Potential biotechnological recycling processes for rubber products include the bacterial degradation of poly(cis-1,4-isoprene) (IR) in order to achieve its total biodegradation or its biotransformation into useful products. The actinomycete Gordonia polyisoprenivorans strain VH2 catalyzes the degradation of IR and enables its use as a sole carbon source via β-oxidation. The initial cleavage reaction is catalyzed by the extracellular latex clearing protein (Lcp). This dioxygenase is the key enzyme for the formation of oligo(cis-1,4-isoprene) molecules with different lengths, i...
March 9, 2018: New Biotechnology
Marilyn C Erickson, Jye-Yin Liao, Mussie Y Habteselassie, Jennifer L Cannon
Field workers often wear gloves harvesting ready-to-eat produce; however, fields are not sterile environments and gloves may become contaminated numerous times during a working shift. This study explored the potential for inactivation of Escherichia coli O157:H7 and Salmonella when contaminated gloves were washed in levulinic acid (LV) and sodium dodecyl sulfate (SDS) solutions. Washing nitrile gloves with increasing concentrations of LV above 1.0% led to a decreased prevalence of glove contamination by Salmonella (P = 0...
August 2018: Food Microbiology
Ignacio Martín-Fabiani, Jennifer Lesage de la Haye, Malin Schulz, Yang Liu, Michelle Lee, Brendan Duffy, Franck D'Agosto, Muriel Lansalot, Joseph L Keddie
The presence of low molar mass surfactants in latex films results in detrimental effects on their water permeability, gloss and adhesion. For applications as coatings, there is a need to develop formulations that do not contain surfactants and that have better water barrier properties. Having previously reported the synthesis of surfactant-free latex particles in water using low amounts (< 2 wt%) of controlled radical polymer chains (Lesage de la Haye et al. Macromolecules 2017, 50, 9315-9328), here we study the water barrier properties of films made from these particles and their application in anti-corrosion coatings...
March 8, 2018: ACS Applied Materials & Interfaces
Binh Pham, Duc Nguyen, Vien Huynh, Eh Hau Pan, Bhavna Shirodkar-Robinson, Michelle Carey, Algirdas Serelis, Gregory G Warr, Tim Davey, Christopher H Such, Brian Stanley Hawkett
A robust polymerization technique that enables the surfactant-free aqueous synthesis of a high solid content latex containing polymeric hollow particles is presented. Uniquely designed amphiphilic macro-RAFT copolymers were used as sole stabilizers for monomer emulsification as well as free radical emulsion polymerization. The polymerization was found to be under RAFT control, generating various morphologies from spherical particles, worm-like structures to polymer vesicles. The final particles were dominantly polymeric vesicles which had a substantially uniform and continuous polymer layer around a single aqueous filled void...
March 8, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Evan Maynard, Emily Heath, Michelle Hilts, Andrew Jirasek
This study introduces the first 3D deformable dosimetry system based on x-ray computed tomography (CT) polymer gel dosimetry (PGD) and establishes the setup reproducibility, deformation characteristics and dose response of the system. A N-isopropylacrylamide (NIPAM)-based gel formulation optimized for X-ray CT gel dosimetry was used, with a latex balloon serving as the deformable container and low-density polyethylene and polyvinyl alcohol providing additional oxygen barrier. Deformable gels were irradiated with a 6 MV calibration pattern to determine dosimetric response and a dosimetrically uniform plan to determine the spatial uniformity of the response...
March 8, 2018: Physics in Medicine and Biology
Izabela Steinka, Anita Kukułowicz
BACKGROUND: Methicillin-Resistant Staphylococcus aureus (MRSA), which is resistant to many antibiotics, creates a serious problem for human health if present in food. This study aimed to assess the quality of commercially-available fresh and frozen strawberries and to compare the behavior of staphylococci in these fruits as affected by the temperature of freezing. METHODS: The research material included different species of fresh strawberries and strawberries frozen with the fluidization method to &ndash;40&deg;C and packaged in an industrial environment...
January 2018: Acta Scientiarum Polonorum. Technologia Alimentaria
Leticia Bignardi, Talita Prates, Andiara De Rossi, Paulo Nelson-Filho, Fabrício Kitasono de Carvalho, Talitha de Siqueira Mellara, Alexandra Mussolino de Queiroz
Myelomeningocele (MMC) is a congenital malformation that occurs in the embryonic period, characterized by failure in closure of the caudal portion of the neural tube during central nervous system formation. Alterations and complications can be associated with this condition, such as hydrocephalus, neurogenic bladder, orthopedic problems, and motor and cognitive impairment. This patients with MMC also have predisposition to develop latex allergy and high caries risk and activity due to deficient oral hygiene, fermentable carbon hydrate-rich diet and prolonged use of sugar-containing oral medications...
March 7, 2018: Special Care in Dentistry
Jacek Kuchinka
The persistent stapedial artery is a component of the main arterial roads of the head in some animal groups (Frąckowiak 2003). This type of vascularization occurs in the Mongolian gerbil, among others. The stapedial artery is common in a variety of forms in rodents. It has been described, for example, in Sciuridae, Muridae, Heteromyidae, Geomyidae, Splacidae, Cricetidae, Arvicolinae, and in genus Jaculus (Cox and Hautier, 2015). The aim of this study was the analysis of morphology, and of course, of the stapedial artery in this species...
March 5, 2018: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
Yukiko Komeno, Yasunori Ota, Tomohiko Koibuchi, Yoichi Imai, Kuniko Iihara, Tomiko Ryu
BACKGROUND Syphilis is a sexually transmitted disease caused by the pathogen Treponema pallidum. Prevalence continues to rise, especially among men who have sex with men (MSM). Due to changes in patterns of sexual activity, manifestations of the disease are highly variable. CASE REPORT A 27-year-old male visited the hospital for a low-grade fever and tender 5-cm mass in the right side of his neck. His right tonsil was swollen and covered with a white coating. Levofloxacin was prescribed, but ineffective. The patient's levels of liver function enzymes increased gradually...
March 4, 2018: American Journal of Case Reports
Lina Paola Orejarena Ávila, Erika Marcela Inguilan Benavides, Leonardo Padilla Sanabria, Delia Piedad Recalde-Reyes, Carlos Andrés Rodríguez-Salazar, Jhon Carlos Castaño-Osorio
Fasciolosis is a zoonotic parasitic disease, which affects humans and animals; diagnosed through noncommercial immunoassay tests that cannot be used on the field. Thereby, establishing the optimal conditions to develop a latex agglutination technique with IgG and IgM antibodies directed against excretion/secretion antigens of Fasciola sp. is a priority. Latex particles were sensitized with IgG and IgM antibodies directed against excretion/secretion antigens of Fasciola sp. The specificity of the antibodies was determined against antigens of different helminths and protozoa; the sensitivity and specificity of the test was evaluated against a previously standardized direct ELISA...
March 2018: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
Aline S L Santoro Soares, Miriam Zaccaro Scelza, Janaína Spoladore, Marcos Antônio Gallito, Felipe Oliveira, Rita de Cássia Martins Moraes, Gutemberg Gomes Alves
Denture adhesives (DA) improve the retention and stability of ill-fitting dentures, especially for older adults. These materials should be biocompatible, i.e., they cannot cause undesired biological responses and be non-cytotoxic to oral tissues. However, in vitro testing of DA biocompatibility employing primary cell culture may possibly be affected by other factors, such as the donor age. OBJECTIVE: To compare the cytotoxicity of three different denture adhesives when assessed in primary gingival fibroblasts from a young donor or from an older donor, as well as the release of the basic fibroblast growth factor (bFGF), and the inflammatory response marker interleukin-6 (IL-6)...
2018: Journal of Applied Oral Science: Revista FOB
Tetsuhito Kigata, Hideshi Shibata
The adrenal gland regulates stress responses by releasing steroid hormones, whose synthesis and secretion are influenced by adrenal blood flow. Adrenalectomy is commonly performed in rabbits to study the function of the adrenal gland. Although knowledge of the arterial supply to the adrenal gland forms the anatomical basis of the surgery, its description in prior studies is incomplete for the rabbit. Therefore, we observed the adrenal arteries in 27 male and 11 female New Zealand White rabbits using the colored latex injection method...
February 27, 2018: Anatomical Science International
U Hagag, M G Tawfiek
The aims of the present study were to describe the normal ultrasonographic, magnetic resonance imaging (MRI) and computed tomographic (CT) appearances of the bovine metacarpo/metatarsophalangeal (MCP/MTP) joints and to assess the normal cross-sectional dimensions of the superficial (SDFT) and deep (DDFT) digital flexor tendons. A systematic ultrasound examination was performed on the MCP/MTP joints of 22 healthy cattle and two bovine cadavers, and the cross-sectional dimensions of the SDFT and DDFT were recorded...
March 2018: Veterinary Journal
G O Nwagbara, Abiodun Mathias Emokpae
Hypertension constitutes one of the major metabolic disease in Nigeria especially among military personnel and their families. Myocardial infarction and other cardiovascular diseases may occur in this group of patient due to uncontrolled or poorly controlled hypertension. The objective of this study was to determine serum cardiac Troponin I (cTnI), levels in hypertensive Nigerian Military service personnel attending clinic in a military Health facility.  We measured the serum levels of cTnI in 126 hypertensive subjects [76 males (19-73 years) and 50 females (26-77years)] and 82 normotensive controls [41 males (19-60years) and 41 females (18-53years)] using Latex Enhanced Immunoturbidimetry technique...
December 30, 2017: Nigerian Journal of Physiological Sciences: Official Publication of the Physiological Society of Nigeria
Camille Boucher-Jacobs, Muhammad Rabnawaz, Joshua S Katz, Ralph Even, Damien Guironnet
The catalytic emulsion polymerization of ethylene has been a long-lasting technical challenge as current techniques still suffer some limitations. Here we report an alternative strategy for the production of semi-crystalline polyethylene latex. Our methodology consists of encapsulating a catalyst precursor within micelles composed of an amphiphilic block copolymer. These micelles act as nanoreactors for the polymerization of ethylene in water. Phosphinosulfonate palladium complexes were used to demonstrate the success of our approach as they were found to be active for hours when encapsulated in micelles...
February 26, 2018: Nature Communications
A B de Sá, L C Oliveira, R Camilo, F F Pierotti, D Solé
Background. Microarray technique employing molecular allergens is pointed out as a new method to evaluate allergic patients. Objective. To evaluate if microarray technique (ImmunoCAP-ISAC®; I-ISAC®) is similar to fluorescence enzyme immunoassay (FEIA; ImmunoCAP®) in the diagnosis of latex allergy (specific IgE to latex plus symptoms) and latex sensitization (only antibody) in pediatric (9-mo to 14-yrs) patients with myelomeningocele undergone to surgical repair. Methods. Patients underwent skin prick testing (SPT) to latex and food (prick to prick), and dosage of serum specific IgE to latex and recombinant latex allergens (rHev b 1, rHev b 3, rHev b 5, rHev b 6...
January 15, 2018: European Annals of Allergy and Clinical Immunology
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