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https://www.readbyqxmd.com/read/27910939/real-time-pcr-based-serotyping-of-streptococcus-agalactiae
#1
Kathleen M Breeding, Bhavana Ragipani, Kun-Uk David Lee, Martin Malik, Tara M Randis, Adam J Ratner
Group B Streptococcus (GBS) is an encapsulated, gram-positive pathogen that is an important cause of neonatal invasive infections, including sepsis and meningitis. There are ten known GBS serotypes based on distinct capsule compositions (Ia, Ib, II-IX), and current candidate capsular polysaccharide conjugate vaccines target only a subset of these. Serotyping of GBS isolates is important for understanding local epidemiology and for monitoring for serotype replacement or capsular switching. However, serotyping generally requires either latex agglutination, multiplex PCR with analysis of band sizes, or analysis of whole genome sequences-all techniques that are either expensive or not widely available...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910545/commercial-counterboard-for-10-ns-software-correlator-for-photon-and-fluorescence-correlation-spectroscopy
#2
Matteo Molteni, Fabio Ferri
A 10 ns time resolution, multi-tau software correlator, capable of computing simultaneous autocorrelation (A-A, B-B) and cross (A-B) correlation functions at count rates up to ∼10 MHz, with no data loss, has been developed in LabVIEW and C++ by using the National Instrument timer/counterboard (NI PCIe-6612) and a fast Personal Computer (PC) (Intel Core i7-4790 Processor 3.60 GHz ). The correlator works by using two algorithms: for large lag times (τ ≳ 1 μs), a classical time-mode scheme, based on the measure of the number of pulses per time interval, is used; differently, for τ ≲ 1 μs a photon-mode (PM) scheme is adopted and the correlation function is retrieved from the sequence of the photon arrival times...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910140/side-effects-of-irinotecan-cpt-11-the-clinically-used-drug-for-colon-cancer-therapy-are-eliminated-in-experimental-animals-treated-with-latex-proteins-from-calotropis-procera-apocynaceae
#3
Nylane Maria Nunes de Alencar, Flávio da Silveira Bitencourt, Ingrid Samantha Tavares de Figueiredo, Patrícia Bastos Luz, Roberto César P Lima-Júnior, Karoline Sabóia Aragão, Pedro Jorge Caldas Magalhães, Gerly Anne de Castro Brito, Ronaldo Albuquerque Ribeiro, Ana Paula Fragoso de Freitas, Marcio Viana Ramos
Intestinal mucositis (IM) is the critical side effect of irinotecan (CPT-11), which is the front-line drug used for the treatment of colorectal cancer. This study aimed to evaluate the effectiveness of latex proteins (LP) from Calotropis procera to prevent IM and diarrhea in animals. Swiss mice were treated daily with saline or LP (1, 5, or 50 mg/kg, i.v.) 24 h prior to CTP-11 (75 mg/kg/4 days, i.p) and for additional 6 days. Animal survival, body weight variation, and diarrhea were registered. After animal sacrifice (day 7 post first injection of CPT-11), intestinal samples were collected to study morphology and inflammatory parameters...
December 2, 2016: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/27903918/reactive-oxygen-species-in-hevea-brasiliensis-latex-and-relevance-to-tapping-panel-dryness
#4
REVIEW
Yi Zhang, Julie Leclercq, Pascal Montoro
Environmental stress can lead to oxidative stress resulting from an increase in reactive oxygen species (ROS) and involves redox adjustments. Natural rubber is synthesized in laticifers, which is a non-photosynthetic tissue particularly prone to oxidative stress. This paper reviews the current state of knowledge on the ROS production and ROS-scavenging systems in laticifers. These regulations have been the subject of intense research into a physiological syndrome, called Tapping Panel Dryness (TPD), affecting latex production in Hevea brasiliensis In order to prevent TPD occurrence, monitoring thiol content appeared to be a crucial factor of latex diagnosis...
November 29, 2016: Tree Physiology
https://www.readbyqxmd.com/read/27903603/multicenter-evaluation-of-a-modified-cefoxitin-disk-diffusion-method-and-pbp2a-testing-to-predict-meca-mediated-oxacillin-resistance-in-atypical-staphylococcus-aureus
#5
Shelley A Miller, James Karichu, Peggy Kohner, Nicolynn Cole, Janet A Hindler, Robin Patel, Sandra Richter, Romney M Humphries
Phenotypic variants of Staphylococcus aureus display small colony size, reduced pigment, hemolysis and/or coagulase activity are periodically isolated by the clinical laboratory. Antimicrobial susceptibility testing (AST) of these isolates is complicated as many do not grow on routine AST media, including Mueller Hinton agar and cation-adjusted Mueller Hinton broth. This multi-center study evaluated cefoxitin disk diffusion for 37 isolates (156 readings) of atypical S. aureus with MHA supplemented with 5% sheep's blood (BMHA), using mecA PCR as the reference standard...
November 30, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27900596/pharmacological-evaluation-for-improvement-of-kanazawa-sutra-medicinal-thread-for-anal-fistula
#6
Takami Yokogawa, Yohei Sasaki, Hirokazu Ando, Katsuya Yamamoto, Masayuki Mikage
Kanazawa Sutra (KanS) is a medicinal thread that is used for the treatment of anal fistula. It is used as a substitute for Kshara Sutra (KS) which is used in Ayurvedic medicine. KanS is composed of Ficus carica latex (FCL), Capsicum annuum tincture (CAT), Achyranthes fauriei Kshara (which is processed ash from the whole plant) and powdered Curcuma longa rhizome (CLR). In this study, we evaluated the ingredients of KanS by measuring nitric oxide (NO) production in murine macrophage-like cell line J774.1 as well as examining cytotoxicity to rat skeletal muscle myoblasts (L6) and L6 differentiation, with a view to improving its pharmacological effect...
November 29, 2016: Journal of Natural Medicines
https://www.readbyqxmd.com/read/27895659/hbmads4-a-mads-box-transcription-factor-from-hevea-brasiliensis-negatively-regulates-hbsrpp
#7
Hui-Liang Li, Li-Ran Wei, Dong Guo, Ying Wang, Jia-Hong Zhu, Xiong-Ting Chen, Shi-Qing Peng
In plants MADS-box transcription factors (TFs) play important roles in growth and development. However, no plant MADS-box gene has been identified to have a function related to secondary metabolites regulation. Here, a MADS-box TF gene, designated as HbMADS4, was isolated from Hevea brasiliensis by the yeast one-hybrid experiment to screen the latex cDNA library using the promoter of the gene encoding H. brasiliensis small rubber particle protein (HbSRPP) as bait. HbMADS4 was 984-bp containing 633-bp open reading frame encoding a deduced protein of 230 amino acid residues with a typical conserved MADS-box motif at the N terminus...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27893628/watershed-areas-in-face-transplantation
#8
Bahar Bassiri Gharb, Russell S Frautschi, Brianna C Halasa, Gaby D Doumit, Risal S Djohan, Steven L Bernard, Brian R Gastman, Maria Z Siemionow, Francis A Papay, Antonio Rampazzo
BACKGROUND: Maxillary artery has traditionally been considered the main blood supply of the facial skeleton. However, the deep and concealed location makes the harvest of facial allografts based on this artery challenging, giving preference to the facial artery. There is growing evidence that the junction between the hard and soft palate may represent a watershed area in facial artery based allografts. The aim of this study was to review the occurrence of partial allograft necrosis and modify the available craniofacial techniques allowing for a reliable harvest of maxillary artery based facial allografts...
November 21, 2016: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/27888641/carotid-artery-and-lower-cranial-nerve-exposure-with-increasing-surgical-complexity-to-the-parapharyngeal-space
#9
Ana M Lemos-Rodriguez, Satyan B Sreenath, Rounak B Rawal, Lewis J Overton, Zainab Farzal, Adam M Zanation
OBJECTIVES/HYPOTHESIS: To investigate the extent of carotid artery exposure attained, including the identification of the external carotid branches and lower cranial nerves in five sequential external approaches to the parapharyngeal space, and to provide an anatomical algorithm. STUDY DESIGN: Anatomical study. METHODS: Six latex-injected adult cadaver heads were dissected in five consecutive approaches: transcervical approach with submandibular gland removal, posterior extension of the transcervical approach, transcervical approach with parotidectomy, parotidectomy with lateral mandibulotomy, and parotidectomy with mandibulectomy...
November 26, 2016: Laryngoscope
https://www.readbyqxmd.com/read/27884872/an-hplc-procedure-for-the-quantification-of-aloin-in-latex-and-gel-from-aloe-barbadensis-leaves
#10
Dalia I Sánchez-Machado, Jaime López-Cervantes, María F Mariscal-Domínguez, Paola Cruz-Flores, Olga N Campas-Baypoli, Ernesto U Cantú-Soto, Ana Sanches-Silva
Aloin is an anthraquinone-C-glycoside present in Aloe vera This compound is extremely variable among different species and highly depends on the growing conditions of the plants. The quantification of aloin in different extraction preparations has been a frequent problem due to the high instability of the compound. The aim of the present study is to develop and validated an analytical method for aloin detection in fresh and dry samples of Aloe barbadensis gel and latex using high performance liquid chromatography coupled to a diode array detector (HPLC-DAD)...
November 24, 2016: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/27881806/how-do-gloves-affect-cutaneous-sensibility-in-medical-practice-two-new-applied-tests
#11
Peter Mylon, Matt J Carré, Nicolas Martin, Roger Lewis
In order to quantify the effect of medical gloves on tactile performance, two new Simulated Medical Examination Tactile Tests (SMETT) have been developed to replicate the tactile and haptic ability required in medical examinations: the 'Bumps' test and the 'Princess and the Pea' (P&P) test. A pilot study was carried out using 30-40 subjects for each test in order to investigate the suitability of the tests for medical glove evaluation. Tests were performed with latex and nitrile examination gloves and without gloves...
November 23, 2016: Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine
https://www.readbyqxmd.com/read/27876984/first-serological-study-of-equine-hydatidosis-in-iran
#12
Ehsanollah Sakhaee, Mehdi Golchin, Hamidreza Amiri, Mohammad Reza Fayed, Jamaladdin Eydi
Hydatidosis, is an important worldwide zoonotic disease caused by larval stages (metacestodes) of tapeworm parasites of the genus Echinococcus. The objective of the present study was to determine the seroprevalence of equine hydatidosis in Iran by latex agglutination test. This study also served to correlate sex and age with mentioned results in cases. Therefore, 193 serum samples were collected from clinically healthy horses at 9 race clubs in Kerman, Yazd and Golestan provinces, Iran. According to the results, antibodies against hydatidosis were detected in 6 sera (3...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27876957/diagnosis-of-cystic-echinococcosis-in-buffaloes-by-native-8%C3%A2-kda-antigen-using-latex-agglutination-test-lat
#13
A Sheeba, A Sangaran, B R Latha
The dog tapeworm Echinococcusgranulosus is the causative agent of cystic hydatid disease in domestic/wild herbivores animals and man. Accurate immunodiagnosis of the infection requires highly specific and sensitive antigens. The aim of this study was to develop and evaluate immunoassays with principles of precipitation, agglutination for the identification of buffaloes infected with hydatid cyst which would allow the monitoring of animals from endemic areas and identifying infected animals prior to slaughter...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27876472/evaluation-of-recombinant-antigens-in-combination-and-single-formula-for-diagnosis-of-feline-toxoplasmosis
#14
Abdelbaset Eweda Abdelbaset, Hend Alhasan, Doaa Salman, Mohamed Hassan Karram, Mahmoud Abd Ellah Rushdi, Xuan Xuenan, Makoto Igarashi
Cats are the only definitive hosts of Toxoplasma gondii and constitute an essential source of infection to all warm blooded animals and humans. Diagnosis of T. gondii infection in cats is fundamental for proper management and control of infection in humans and animals. In the current study, we have evaluated the diagnostic performance of tachyzoite lysate antigen (TLA) and different T. gondii recombinant antigens including surface antigen 2 (SAG2), dense granule proteins 2, 6, 7, 15 (GRA2, GRA6, GRA7, GRA15) and microneme 10 protein (MIC10) in immunoglobulin G enzyme linked-immunosorbent assay (IgG ELISA) using cat serum samples, with reference to latex agglutination test (LAT)...
November 19, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27876296/pathogenic-features-of-clinically-significant-coagulase-negative-staphylococci-in-hospital-and-community-infections-in-benin
#15
Chimène Nanoukon, Xavier Argemi, Frédéric Sogbo, Jeanne Orekan, Daniel Keller, Dissou Affolabi, Frédéric Schramm, Philippe Riegel, Lamine Baba-Moussa, Gilles Prévost
In West Africa, very little consideration has been given to coagulase negative Staphylococci (CNS). Herein, we describe the features contributing to the pathogenicity of 99 clinically-significant independent CNS isolates associated with infections encountered at the National Teaching Hospital Center of Cotonou (Benin). The pathogenic potentials of nosocomial strains were compared with community strains. S. haemolyticus (44%), S. epidermidis (22%) and S. hominis (7%) were the most frequently isolated while bacteremia (66...
November 10, 2016: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/27875955/detection-the-mycoplasma-pneumonia-in-synovial-fluid-of-children-with-negative-culture-arthritis-a-cross-sectional-study-in-tehran-iran
#16
Samileh Noorbakhsh, Mohammad Reza Shokrollahi, Farideh Ebrahimi Taj, Vida Zarabi, Azardokht Tabatabaei
BACKGROUND: arthritis could be caused by different etiologies ranging from rheumatologic diseases to infectious conditions. Therefore, early diagnosis of etiology and treatment is important. The purpose of this study was to determine the the M.pneumonia in synovial fluid of children with arthritis by 2 methods (serology and qualitative PCR). Methods & materials: this trial was carried out as a cross sectional study in pediatric and orthopedic ward of Rasoul-e Akram hospital in Tehran, Iran...
November 21, 2016: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/27873521/a-case-of-recurrent-urticaria-due-to-formaldehyde-release-from-root-canal-disinfectant
#17
Ji Hoon Jang, Seung Hyun Park, Hang Jea Jang, Sung Geun Lee, Jin Han Park, Jae Won Jeong, Chan Sun Park
Although formaldehyde is well known to cause type 4 hypersensitivity, immunoglobulin E (IgE)-mediated hypersensitivity to formaldehyde is rare. Here, we report a case of recurrent generalized urticaria after endodontic treatment using a para-formaldehyde (PFA)-containing root canal sealant and present a review of previous studies describing cases of immediate hypersensitivity reactions to formaldehyde. A 50-year-old man visited our allergy clinic for recurrent generalized urticaria several hours after endodontic treatment...
January 2017: Yonsei Medical Journal
https://www.readbyqxmd.com/read/27872990/blood-supply-of-the-subacromial-bursa-and-rotator-cuff-tendons-on-the-bursal-side
#18
Elle Põldoja, Madis Rahu, Kristo Kask, Imke Weyers, Ivo Kolts
PURPOSE: Vascularity of the subacromial bursa and rotator cuff tendons is key factors in the pathogenesis of subacromial bursitis and impingement syndrome, rotator cuff tendinitis, and rotator cuff tears. The purpose of this study was to investigate and describe blood supply to the cranial and caudal parts of the subacromial bursa and the vascularity of the rotator cuff tendons on the bursal side. METHODS: Fourteen fresh cadaveric shoulders from six females and eight males with a mean age of 71...
November 21, 2016: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/27871841/rubber-particle-proteins-ref1-and-srpp1-interact-differently-with-native-lipids-extracted-from-hevea-brasiliensis-latex
#19
Kanthida Wadeesirisak, Sabine Castano, Karine Berthelot, Laurent Vaysse, Frédéric Bonfils, Frédéric Peruch, Kittipong Rattanaporn, Siriluck Liengprayoon, Sophie Lecomte, Céline Bottier
Rubber particle membranes from the Hevea latex contain predominantly two proteins, REF1 and SRPP1 involved in poly(cis-1,4-isoprene) synthesis or rubber quality. The repartition of both proteins on the small or large rubber particles seems to differ, but their role in the irreversible coagulation of the rubber particle is still unknown. In this study we highlighted the different modes of interactions of both recombinant proteins with different classes of lipids extracted from Hevea brasiliensis latex, and defined as phospholipids (PL), glycolipids (GL) and neutral lipids (NL)...
November 19, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27869321/performance-and-selectivity-of-cationic-nanoparticle-pseudo-stationary-phases-in-electrokinetic-chromatography
#20
Julie R McGettrick, Nathan H Williamson, Adam T Sutton, Christopher P Palmer
Electrokinetic chromatography (EKC) is a powerful analytical technique that uses an ionic pseudo-stationary phase (PSP) to separate neutral compounds. Although anionic surfactants are the most common choice for PSP, cationic latex nanoparticles are an attractive alternative. Reversible addition-fragmentation chain transfer (RAFT) polymerization was used to synthesize several types of diblock copolymers that self-assemble into latex nanoparticles, which were characterized by a variety of techniques including diffusion NMR...
November 21, 2016: Electrophoresis
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