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https://www.readbyqxmd.com/read/28727605/in-vitro-compatibility-of-contact-lenses-with-corneal-epithelial-cells
#1
Ajay K Vijay, Zohra Fadli, Carol Lakkis, Chantal Coles-Brennan, Mark D P Willcox
PURPOSE: To determine the interaction of contact lenses of different materials with corneal epithelial cells grown in tissue culture. METHODS: Two different corneal epithelial cell lines were grown to confluence in culture media. Two hydrogel contact lenses with and without polyvinylpyrrolidone (PVP) {1-DAY ACUVUE MOIST (1-Day ACUVUE [hydrogel lenses]) and a silicone hydrogel contact lens, AIR OPTIX NIGHT & DAY} were removed from their blister packs, washed in phosphate-buffered saline, and applied to the cells...
July 18, 2017: Eye & Contact Lens
https://www.readbyqxmd.com/read/28723191/highly-enriched-controllable-continuous-aerosol-sampling-using-inertial-microfluidics-and-its-application-to-real-time-detection-of-airborne-bacteria
#2
Jeongan Choi, Seung Chan Hong, Woojin Kim, Jae Hee Jung
We report a novel microfluidic technique for sampling of aerosols into liquids. The two-phase fluid, sampling air and collecting liquid, forms a stratified flow in the curved microchannel. By passing fluids through the curved region, the particles are transferred from air into the liquid phase by the particle centrifugal and drag forces. This microfluidic-based aerosol-into-liquid sampling system, called the MicroSampler, is driven by particle inertial differences. To evaluate the physical particle collection efficiency of the MicroSampler, we used standard polystyrene-latex (PSL) particles ranging in size from 0...
April 28, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28719186/elastomer-reinforced-with-regenerated-chitin-from-alkaline-urea-aqueous-system
#3
Peng Yu, Hui He, Yuanfang Luo, Demin Jia, Alain Dufresne
Novel hybrid elastomer/regenerated chitin (R-chitin) composites were developed, for the first time, by introducing chitin solution (dissolved in alkaline/urea aqueous solution at low temperature) into rubber latex, and then co-coagulating using ethanol as the co-coagulant. During the rapid co-precipitation process, the chitin solution showed rapid coagulant-induced gelation and a porous chitin phase was generated, while the rubber latex particles were synchronously demulsificated to form the rubbery phase. The two phases interlaced and interpenetrated simultaneously to form an Interpenetrating Polymer Network (IPN) structure, which was evidenced by SEM observation...
July 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28718699/multiple-facets-of-laticifer-cells
#4
Lourdes Castelblanque, Begoña Balaguer, Cristina Martí, Juan José Rodríguez, Marianela Orozco, Pablo Vera
In the latex-bearing plants, the laticiferous system is the tubing structure that contains the latex and is constituted of living cells (laticifers). While laticifers are present only in a small percentage of the flowering plant species, they represent a type of specialized tissue within the plant where a myriad of metabolites are synthesized, some of them of considerable commercial importance. In this mini-review we synopsize the present knowledge about laticifer cells and discuss about their particular features as well as some evolutionary and ecophysiological cues and the potential exploitation of the knowledge generated around this peculiar type of plant cell...
July 18, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28716430/non-invasive-evaluation-of-right-ventricular-function-with-real-time-3-d-echocardiography
#5
Ran Chen, Meihua Zhu, Kacie Amacher, Xia Wu, David J Sahn, Muhammad Ashraf
The aim of this study was to evaluate the accuracy and feasibility of real-time 3-D echocardiography (3-DE) in assessing right ventricular (RV) systolic function. A latex balloon was inserted into the right ventricle of 20 freshly harvested pig hearts which were then passively driven by a pulsatile pump apparatus. The RV global longitudinal strain (GLS), global circumferential strain (GCS), global area strain (GAS) and RV ejection fraction (RVEF), derived from 3-DE, as well as the RVEF obtained from 2-D echocardiography (2-DE) were quantified at different stroke volumes (30-70 mL) and compared with sonomicrometry data...
July 14, 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28711667/identification-of-three-homologous-latex-clearing-protein-lcp-genes-from-the-genome-of-streptomyces-sp-strain-cfmr-7
#6
Jayaram Nanthini, Su Yean Ong, Kumar Sudesh
Rubber materials have greatly contributed to human civilization. However, being a polymeric material does not decompose easily, it has caused huge environmental problems. On the other hand, only few bacteria are known to degrade rubber, with studies pertaining them being intensively focusing on the mechanism involved in microbial rubber degradation. The Streptomyces sp. strain CFMR 7, which was previously confirmed to possess rubber-degrading ability, was subjected to whole genome sequencing using the single molecule sequencing technology of the PacBio® RS II system...
July 12, 2017: Gene
https://www.readbyqxmd.com/read/28709944/latex-proteins-from-calotropis-procera-toxicity-and-immunological-tolerance-revisited
#7
Camila F Bezerra, Érika F Mota, Ana Claudia M Silva, Adriana R Tomé, Maria Z R Silva, Daniel de Brito, Camila T M N Porfírio, Ariclécio C Oliveira, José V Lima-Filho, Márcio V Ramos
Many thousands of plants are disseminated worldwide in traditional and folk medicines based on the belief that their leaves, roots, seeds, bark or secretions, when adequately handled, can treat, alleviate or ameliorate numerous disease symptoms. Calotropis procera (Apocynaceae) is a popular medicinal plant and the claims of this shrub's phytomedicinal properties have been scientifically validated. In this study, further prospects towards the in vivo toxicity and oral immunological tolerance of phytomodulatory proteins isolated from the latex of C...
July 11, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28706952/development-of-an-economical-method-to-reduce-the-extractable-latex-protein-levels-in-finished-dipped-rubber-products
#8
Ambegoda Liyanage Harini Amalka Perera, Bulathsinhalage Gayani Kanchana Perera
Natural rubber latex (NRL) allergy is caused by the extractable latex proteins in dipped rubber products. It is a major concern for the consumers who are sensitive to the allergenic extractable proteins (EP) in products such as NRL gloves. Objective of this research was to develop an economical method to reduce the EP in finished dipped NRL products. In order to reduce the EP levels, two natural proteases, bromelain from pineapple and papain from papaya, were extracted and partially purified using (NH4)2SO4...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28701251/recognition-and-management-of-anaphylaxis-in-older-adults
#9
Anastasiya Plagova, Diane Nykamp
OBJECTIVES: To discuss medications that cause anaphylaxis in older adults and its treatment, with a focus on epinephrine. DATA SOURCE: A search of PubMed using the terms "anaphylaxis," "drug-induced anaphylaxis," "epinephrine autoinjectable," "anaphylaxis diagnosis," "anaphylaxis treatment," and "epinephrine cost" was performed. English-language articles from January 2000 to August 2016 appearing in these searches were reviewed. STUDY SELECTION/DATA EXTRACTION: Relevant review articles, consensus statements, practice parameters, task force recommendations, and original articles were assessed for recognition and treatment of drug-induced anaphylaxis...
July 1, 2017: Consultant Pharmacist: the Journal of the American Society of Consultant Pharmacists
https://www.readbyqxmd.com/read/28700079/study-of-the-surfactant-role-in-latex-aerogel-systems-by-scanning-transmission-electron-microscopy-on-aqueous-suspensions
#10
A Perret, G Foray, K Masenelli-Varlot, E Maire, B Yrieix
For insulation applications, boards thinner than 2 cm are under design with specific thermal conductivities lower than 15 mW m(-1)  K(-1) . This requires binding slightly hydrophobic aerogels which are highly nanoporous granular materials. To reach this step and ensure insulation board durability at the building scale, it is compulsory to design, characterise and analyse the microstructure at the nanoscale. It is indeed necessary to understand how the solid material is formed from a liquid suspension. This issue is addressed in this paper through wet-STEM experiments carried out in an Environmental Scanning Electron Microscope (ESEM)...
July 12, 2017: Journal of Microscopy
https://www.readbyqxmd.com/read/28699390/randomized-controlled-trial-of-chest-tube-removal-aided-by-a-party-balloon
#11
Puwadon Thitivaraporn, Natawat Narueponjirakul, Pasurachate Samorn, Supparerk Prichayudh, Sukanya Sriussadaporn, Rattaplee Pak-Art, Suvit Sriussadaporn, Kritaya Kritayakirana
Background Recurrent pneumothorax is one of the most common complications after thoracostomy tube removal. The purpose of this study was to assess the optimal method of thoracostomy tube removal by comparing party balloon-assisted Valsalva and classic Valsalva techniques. Methods Trauma patients with indications for tube thoracostomy from 2014 to 2015 were recruited. Exclusion criteria were age < 15- or > 64-years-old, history of chronic lung disease, Glasgow Coma Scale < 13, latex allergy, or tracheostomy...
January 1, 2017: Asian Cardiovascular & Thoracic Annals
https://www.readbyqxmd.com/read/28698893/cytotoxicity-against-tumor-cell-lines-and-anti-inflammatory-properties-of-chitinases-from-calotropis-procera-latex
#12
Carolina Araújo Viana, Márcio V Ramos, José Delano Barreto Marinho Filho, Letícia Veras Costa Lotufo, Ingrid Samantha Tavares Figueiredo, Jefferson Soares de Oliveira, Pietro Mastroeni, José Vitor Lima-Filho, Nylane Maria Nunes Alencar
The role of chitinases from the latex of medicinal shrub Calotropis procera on viability of tumor cell lines and inflammation was investigated. Soluble latex proteins were fractionated in a CM Sepharose Fast-Flow Column and the major peak (LPp1) subjected to ion exchange chromatography using a Mono-Q column coupled to an FPLC system. In a first series of experiments, immortalized macrophages were cultured with LPp1 for 24 h. Then, cytotoxicity of chitinase isoforms (LPp1-P1 to P6) was evaluated against HCT-116 (colon carcinoma), OVCAR-8 (ovarian carcinoma), and SF-295 (glioblastoma) tumor cell lines in 96-well plates...
July 11, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/28695652/production-of-functionalized-oligo-isoprenoids-by-enzymatic-cleavage-of-rubber
#13
Wolf Röther, Jakob Birke, Stephanie Grond, Jose Manuel Beltran, Dieter Jendrossek
In this study, we show the proof of concept for the production of defined oligo-isoprenoids with terminal functional groups that can be used as starting materials for various purposes including the synthesis of isoprenoid-based plastics. To this end, we used three types of rubber oxygenases for the enzymatic cleavage of rubber [poly(cis-1,4-isoprene)]. Two enzymes, rubber oxygenase RoxAXsp and rubber oxygenase RoxBXsp , originate from Xanthomonas sp. 35Y; the third rubber oxygenase, latex-clearing protein (LcpK30 ), is derived from Gram-positive rubber degraders such as Streptomyces sp...
July 11, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28695221/core-shell-nano-latex-blending-method-to-prepare-multi-shape-memory-polymers
#14
Hongze Li, Yingwu Luo, Xiang Gao
Multi-shape memory polymers were prepared by blending a series of styrene (St)-block-(styrene-random-meth acrylate (MA))-block-styrene triblock copolymer nano-latexes. These latexes synthesized using a reversible addition-fragmentation chain transfer (RAFT) emulsion polymerization method have a core-shell structure. The cores are formed by the middle poly(St-random-MA) blocks. They act as transition phases with variable transition temperatures via adjusting the St/MA ratio. When the latexes are blended with an identical PSt shell but different poly(St-random-MA) cores, the shells play a role in preventing the aggregation of these poly(St-random-MA) cores forming a crosslinked network after hot-press treatment...
July 11, 2017: Soft Matter
https://www.readbyqxmd.com/read/28692480/association-of-plasminogen-activator-inhibitor-1-and-low-density-lipoprotein-heterogeneity-as-a-risk-factor-of-atherosclerotic-cardiovascular-disease-with-triglyceride-metabolic-disorder-a-pilot-cross-sectional-study
#15
Kiyoshi Iida, Shigemasa Tani, Wataru Atsumi, Tsukasa Yagi, Kenji Kawauchi, Naoya Matsumoto, Atsushi Hirayama
BACKGROUND: We hypothesized that an increase in plasminogen activator inhibitor 1 (PAI-1) might reduce low-density lipoprotein (LDL) particle size in conjunction with triglyceride (TG) metabolism disorder, resulting in an increased risk of atherosclerotic cardiovascular disease (ASCVD). METHODS: This study was carried out as a hospital-based cross-sectional study in 537 consecutive outpatients (mean age: 64 years; men: 71%) with one or more risk factors for ASCVD from April 2014 to October 2014 at the Cardiovascular Center of Nihon University Surugadai Hospital...
July 7, 2017: Coronary Artery Disease
https://www.readbyqxmd.com/read/28690968/miniemulsion-copolymerization-of-meth-acrylates-in-the-presence-of-functionalized-multiwalled-carbon-nanotubes-for-reinforced-coating-applications
#16
Bertha T Pérez-Martínez, Lorena Farías-Cepeda, Víctor M Ovando-Medina, José M Asua, Lucero Rosales-Marines, Radmila Tomovska
Film forming, stable hybrid latexes made of methyl metacrylate (MMA), butyl acrylate (BA) and 2-hydroxyethyl methacrylate (HEMA) copolymer reinforced with modified multiwalled carbon nanotubes (MWCNTs) were synthesized by in situ miniemulsion polymerization. The MWCNTs were pretreated by an air sonication process and stabilized by polyvinylpyrrolidone. The presence of the MWCNTs had no significant effect on the polymerization kinetics, but strongly affected the polymer characteristics (Tg and insoluble polymer fraction)...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28689674/determination-of-immune-status-in-dogs-against-cpv-2-by-recombinant-protein-based-latex-agglutination-test
#17
Jobin Thomas, Mithilesh Singh, T K Goswami, Philma Glora, Soumendu Chakravarti, Vishal Chander, Vikramaditya Upmanyu, Suman Verma, Chhavi Sharma, K Mahendran
Canine parvoviral enteritis is a highly contagious viral illness caused by canine parvovirus-2 (CPV-2) which affects puppies of mainly 6-20 weeks of age. Vaccination is pivotal in preventing and controlling CPV-2 infection. Determination of antibody status is a critical determinant for successful vaccination. The hemagglutination inhibition (HI) test is 'gold standard' test for quantification of antibodies specific to CPV-2, although the execution of this test is not feasible under field conditions. The present study was undertaken to develop a point of care testing to determine immune status prior to CPV-2 vaccination or to detect seroconversion in immunized dogs by latex agglutination test (LAT) using recombinant antigen...
July 6, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28681813/is-there-a-need-to-revise-the-antibiotic-concentration-in-clinical-and-laboratory-standards-institute-recommended-oxacillin-screen-agar
#18
Niveditha Nagasundaram, Sujatha Sistla
INTRODUCTION: In routine diagnostic microbiology laboratories, Clinical and Laboratory Standards Institute (CLSI) recommends the use of cefoxitin disc, in addition to oxacillin screen agar (OSA) of 6 μg/ml for the detection of methicillin-resistant Staphylococcus aureus (MRSA), whereas minimum inhibitory concentration values of oxacillin for S. aureus are ≤2 μg/ml (susceptible) and ≥4 μg/ml (resistant). Hence, the study was carried out to evaluate the ability of screen agar with lower concentrations of oxacillin to identify the isolates of MRSA and to compare this with cefoxitin disc diffusion (CDD)...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28681434/arterial-patterns-of-the-face-in-perissodactyla
#19
Karolina Kowalczyk, Hieronim Frąckowiak
Considerable consistency in the arterial pattern of the head has been observed in species of Artiodactyla, but few studies have examined the order Perissodactyla. Here we describe arteries supplying the intermandibular, mental, masseteric, buccal, labial and nasal regions in eight perissodactylans, including representing of all families comprising this order. Observations were made on a total of 45 preparations of head arteries, obtained by injection of arteries with acetone-dissolved stained vinyl superchloride or stained latex LBS3060...
July 6, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/28671578/preparation-of-self-assembled-chitin-nanofiber-natural-rubber-composite-sheets-and-porous-materials
#20
Akito Kawano, Kazuya Yamamoto, Jun-Ichi Kadokawa
We previously reported the preparation of a self-assembled chitin nanofiber (CNF) film via regeneration from an ion gel with an ionic liquid, followed by sonication and filtration. Based on the finding that CNFs were redispersed in a mixture of the film with ammonia aqueous solution (aq.), in this study, CNF-natural rubber (NR) composite sheets were fabricated by mixing redispersed CNF with NR latex stabilized by ammonia, followed by drying under reduced pressure. Tensile testing of the sheets indicated the reinforcing effect of CNFs...
July 1, 2017: Biomolecules
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