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E De Crop, J Nuytinck, K Van de Putte, K Wisitrassameewong, J Hackel, D Stubbe, K D Hyde, M Roy, R E Halling, P-A Moreau, U Eberhardt, A Verbeken
Infrageneric relations of the genetically diverse milkcap genus Lactifluus (Russulales, Basidiomycota) are poorly known. Currently used classification systems still largely reflect the traditional, mainly morphological, characters used for infrageneric delimitations of milkcaps. Increased sampling, combined with small-scale molecular studies, show that this genus is underexplored and in need of revision. For this study, we assembled an extensive dataset of the genus Lactifluus, comprising 80 % of all known species and 30 % of the type collections...
June 2017: Persoonia
D Baud, N Vulliemoz, A Ammerdorffer, J Gyger, G Greub, V Castella, M Stojanov
STUDY QUESTION: What is the impact of Waddlia chondrophila, an emerging Chlamydia-related bacterium associated with miscarriage, on human spermatozoa? SUMMARY ANSWER: W. chondrophila had a negative impact on human spermatozoa (decrease in viability and mitochondrial membrane potential) and was not entirely removed from infected samples by density gradient centrifugation. WHAT IS KNOWN ALREADY: Bacterial infection or colonization might have a deleterious effect on male fertility...
November 14, 2017: Human Reproduction
Lucas de Araújo Bastos Santana, Diego Passos Aragão, Thiago de Souza Lopes Araújo, Nayara Alves de Sousa, Luan Kelves Miranda de Souza, Lucas Eduardo Silva Oliveira, Anna Carolina Toledo da Cunha Pereira, Gustavo Portela Ferreira, Naylla Veras de Moraes Oliveira, Bruna da Silva Souza, Francisca Beatriz Melo Sousa, Márcio Viana Ramos, Cleverson Diniz Teixeira de Freitas, Jand-Venes Rolim Medeiros, Jefferson Soares de Oliveira
The water-soluble protein fraction obtained from Plumeria pudica (LPPp) latex has previously been demonstrated to have anti-inflammatory and antinociceptive effects. In the present study, LPPp was tested for activity against diarrhea induced by castor oil, prostaglandin E2 (PGE2) or cholera toxin. Different doses of LPPp (10, 20 or 40mg/kg) significantly inhibited the percentage of diarrheal stools (31.18%, 42.97% and 59.70%, respectively) induced by castor oil. This event was followed by significant reduction of both intestinal fluid accumulation (31...
November 10, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Zhoubing Huang, Shuqin Liu, Jiangqiao Xu, Li Yin, Fen Sun, Ningbo Zhou, Gangfeng Ouyang
The introduction of functional groups into metal organic frameworks has great potential for enhancement in adsorption performance of nitrosamines using solid phase microextraction (SPME). However, pre-functionalization and post-modification generally suffer from the same limitation that the additional functional groups occupy the free volume and thereby decreasing pore volume and special surface area. Herein, we use a modulator-induced defect-formation strategy to prepare the 8-aminocaprylic acid doped UIO-66 (Am-UIO-66) with high adsorption capacity of nitrosamines...
February 1, 2018: Talanta
Ning Yan, Xiao-Fang Wan, Xin-Sheng Chai, Run-Quan Chen, Chun-Xia Chen
This paper reports on an ionic-liquid assisted headspace gas chromatographic (HS-GC) for the determination of the content of alkyl ketene dimer (AKD) in its latex samples, in which the GC system was equipped with a thermal conductivity detector (TCD). The method was based on the AKD hydrolysis conducted in 1-butyl-3-methylimidazolium chloride (ionic-liquid) added medium at 100°C for 10min in a closed headspace sample vial, and the measured CO2 (the resulting product of the hydrolysis) by HS-GC. The results showed that the present method has a good measurement precision (RSD <2...
November 7, 2017: Journal of Chromatography. A
Yatao Yin, Shuwei Qiu, Xiangpen Li, Bo Huang, Yun Xu, Ying Peng
BACKGROUND: Glioblastoma multiforme (GBM) induces tumor immunosuppression through interacting with tumor-infiltrating microglia or macrophages (TAMs) with an unclear pathogenesis. Enhancer of zeste homolog 2 (EZH2) is abundant in GBM samples and cell lines and is involved in GBM proliferation, cell cycle, and invasion, whereas its association with innate immune response is not yet reported. Herein, the aim of this study was to investigate the role of EZH2 in GBM immune. METHODS: Co-culturing models of human/murine GBM cells with PBMC-derived macrophages/primary microglia were employed...
November 13, 2017: Journal of Neuroinflammation
Almaky Almagirby, Jem A Rongong, Matt J Carré
Prolonged exposure of the hand to tool-induced vibrations is associated with the occurrence of conditions such as vibration white finger. This study involves the development of a new artificial model that approximates both loading and vibration behaviour of the human finger. The layered system uses polypropylene "bones", encased in a cylinder of low modulus, room-temperature curing silicone gel (to replicate subcutaneous tissues), with an outer layer of latex (to replicate the dermis and epidermis). A protocol for manufacture was developed and dynamic mechanical analysis was carried out on a range of gels in order to choose a range close to the mechanical properties of the human finger...
November 4, 2017: Journal of the Mechanical Behavior of Biomedical Materials
De-Li Zhai, Haiying Yu, Si-Chong Chen, Sailesh Ranjitkar, Jianchu Xu
The phenology of rubber trees (Hevea brasiliensis) could be influenced by meteorological factors and exhibits significant changes under different geoclimates. In the sub-optimal environment in Xishuangbanna, rubber trees undergo lengthy periods of defoliation and refoliation. The timing of refoliation from budburst to leaf aging could be affected by powdery mildew disease (Oidium heveae), which negatively impacts seed and latex production. Rubber trees are most susceptible to powdery mildew disease at the copper and leaf changing stages...
November 13, 2017: International Journal of Biometeorology
Alicia Arechavala, Ricardo Negroni, Fernando Messina, Mercedes Romero, Emmanuel Marín, Roxana Depardo, Laura Walker, Gabriela Santiso
BACKGROUND: Cryptococcosis is still a life-threatening mycosis that continues to be of serious concern in Latin American countries, especially among HIV+positive population. However, there is not any reliable information about the prevalence of this disease in this region. AIMS: The aim of this study is to report data of 2041 patients with cryptococcosis that were attended at the Infectious Diseases Hospital F. J. Muñiz over a 30 year-period. METHODS: Information about demographic and clinical data, survival time and the applied treatment, was taken from the Mycology Unit database...
November 9, 2017: Revista Iberoamericana de Micología
Lidija Kocbek, Mateja Rakuša
PURPOSE: The right bronchial artery usually arises from the descending thoracic aorta as a common trunk with the right intercostal artery and forms the right intercostobronchial trunk. Both, the third right posterior intercostal artery and the right intercostobronchial trunk, are described as the most constant vessels. The focus of the study was to determine the characteristics of the right intercostobronchial trunk regarding the origins of the posterior intercostal arteries from the thoracic aorta...
November 10, 2017: Surgical and Radiologic Anatomy: SRA
Kyunil Rah, Sujeong Han, Jaeyeong Choi, Chul Hun Eum, Seungho Lee
We investigate an explicit role of the ionic strength in the retention behaviors of polystyrene (PS) latex particles in sedimentation field-flow fractionation (SdFFF) by hinging upon the retention theory recently developed [1] asR=(Ro+vb(*))/(1+vb(*)). Here R is an experimental retention ratio, and Ro is the analytical expression of the standard retention theory based on the parabolic flow velocity. The reduced boundary velocityvb(*) is expressed in terms of the ionic strength I of the carrier liquid as vb(*)=vb,o(*)/(1+εI), where vb,o(*)=0...
November 7, 2017: Journal of Chromatography. A
Weijia Fan, Masatoshi Tosaka, Shigeru Yamago, Michael F Cunningham
Ab initio emulsion polymerization of methyl methacrylate (MMA) using a water-soluble organotellurium chain transfer agent in the presence of the surfactant Brij 98 in water is reported. Polymerization proceeded under both thermal and visible light-irradiation conditions giving poly(methyl methacrylate) (PMMA) with controlled molecular weight and low dispersity (Đ<1.5). Despite the formation of an opaque latex, the photo-activation of the organotellurium dormant species took place efficiently as demonstrated by the quantitative monomer conversion and temporal control...
November 10, 2017: Angewandte Chemie
Peter Mandl, David Bong, Peter V Balint, Hilde Berner Hammer, Maribel Miguel, Esperanza Naredo, Lene Terslev, Ingrid Möller
Our study provides a detailed anatomic and sonographic description of the subtalar joint, a single joint that, anatomically, is divided into the anterior subtalar joint (ASTJ) and the posterior subtalar joint (PSTJ). Cadaver specimens of the ankle and foot were examined in detail by ultrasound (US), and the subtalar joints of all the specimens were injected with colored latex of a contrasting color under US guidance. Compatible with other studies, examination of the sections revealed lack of communication between the ASTJ and the PSTJ and communication between the PSTJ and the posterior recess of the tibiotalar joint...
November 6, 2017: Ultrasound in Medicine & Biology
Ameerah Y Mansour
OBJECTIVES: To compare orthodontic elastics with different force extension characteristics, thereby aiming to reduce the orthodontist's inventory for elastics. MATERIALS AND METHODS: Thirty nonextraction class I models were used to determine extension distances. Light, medium, and heavy forced Latex elastics of size 1/4 and 3/16 (from three manufacturers) were used. Thirty elastics from each pack were selected, for a total sample size of 540 elastics. Elastic force was measured at three extensions: three times the lumen (A), first molar to opposing canine (B), and second molar to opposing canine distance (C)...
October 2017: Journal of Orthodontic Science
Masayuki Inui, Susumu Ohwada, Yoshikatsu Inui, Yuko Kondo
The serum Helicobacter pylori titers are good markers for population-based H. pylori screening and treatment programs because the tests used to estimate these titers are noninvasive, inexpensive, and convenient. However, the reference range of 3-10U/mL, which is used as a standard in commercially available serum H. pylori antibody kit E plate Eiken H. pylori antibody II, is regarded as a gray-zone cutoff value to indicate H. pylori infection status. We aimed to clarify the gray-zone cutoff values of H. pylori infection status with new serum H...
2017: Nihon Shokakibyo Gakkai Zasshi, the Japanese Journal of Gastro-enterology
Tatiana A Nevzorova, Qingze Zhao, Yakov A Lomakin, Anastasia A Ponomareva, Alexander R Mukhitov, Prashant K Purohit, John W Weisel, Rustem I Litvinov
Interactions of DNA with proteins are essential for key biological processes and have both a fundamental and practical significance. In particular, DNA binding to anti-DNA antibodies is a pathogenic mechanism in autoimmune pathology, such as systemic lupus erythematosus. Here we measured at the single-molecule level binding and forced unbinding of surface-attached DNA and a monoclonal anti-DNA antibody MRL4 from a lupus erythematosus mouse. In optical trap-based force spectroscopy, a microscopic antibodycoated latex bead is trapped by a focused laser beam and repeatedly brought into contact with a DNA-coated surface...
March 2017: BioNanoScience
Sujal I Shah, Hai Bui, Nelson Velasco, Shilpa Rungta
BACKGROUND Cryptococcus is the third most common invasive fungal organism in immunocompromised patients, including transplant patients, and usually involves the central nervous system and lungs, with a median time to infection of 25 months. We report a case of Cryptococcus of the prostate gland, found as an incidental finding on prostate biopsy for prostate adenocarcinoma, four months following cardiac transplantation. CASE REPORT A 62-year-old male African-American who had a cardiac transplant four months previously, underwent a six-core prostate biopsy for a two-year history of increasing prostate-specific antigen (PSA) levels, and a recent history of non-specific urinary tract symptoms...
November 6, 2017: American Journal of Case Reports
Benjamin A Peprah, Juliana A Ramsay, Bruce A Ramsay
The effects of carboxylation (via mercaptoundecanoic acid) on colloidal properties of medium-chain-length poly(3-hydroxyalkanoate) (mcl-PHA) latexes were studied. Non-ionic surfactants tested at 0.4% solids of 11 mol% carboxylated mcl-PHA produced similar particle sizes and particle size distribution (PdI) with Triton X-100 giving the smallest size. When Triton X-100 was combined with an ionic surfactant, smaller nanoparticles (97.1±1.1 to 121.7±5.7nm) with narrower PdIs (0.21±0.001 to 0.25±0.003) were obtained...
November 1, 2017: International Journal of Biological Macromolecules
Manuel Zorzi, Cesare Hassan, Giulia Capodaglio, Chiara Fedato, Adriana Montaguti, Anna Turrin, Alberto Rosano, Daniele Monetti, Carmen Stocco, Susanna Baracco, Francesca Russo, Alessandro Repici, Massimo Rugge
BACKGROUND: The long-term performance of colorectal cancer (CRC) screening programmes based on a 2-year faecal immunochemical test (FIT) is still unclear. METHODS: In a sample of 50 to 69-year-olds repeatedly screened with the FIT (OC-Hemodia latex agglutination test; cut-off: 20 µg haemoglobin/g faeces), we examined: (1) the FIT positivity rate, the CRC and advanced adenoma detection rate and the FIT's positive predictive value (PPV) for advanced neoplasia, at each round of screening and (2) the cumulative CRC and advanced adenoma detection rate after five rounds of FIT...
November 3, 2017: Gut
Michel Doumith, Shazad Mushtaq, Veronique Martin, Aiysha Chaudhry, Rachael Adkin, Juliana Coelho, Vicki Chalker, Alasdair MacGowan, Neil Woodford, David M Livermore
Background: Like other streptococci, Streptococcus agalactiae typically has intrinsic low-level aminoglycoside resistance. High-level gentamicin resistance was seen in 2 of 1125 isolates collected in the BSAC Bacteraemia Surveillance Programme between 2001 and 2014. These organisms, both isolated in 2014, were characterized. Methods: Identifications were by latex agglutination, MICs by BSAC agar dilution and sequencing by Illumina methodology. Results: Gentamicin MICs were >1024 mg/L versus a species mode of 8 mg/L; both isolates also were unusually ciprofloxacin resistant with MICs of 64 mg/L versus a species mode of 1 mg/L...
October 1, 2017: Journal of Antimicrobial Chemotherapy
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