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https://www.readbyqxmd.com/read/28917307/evaluation-of-western-blot-elisa-and-latex-agglutination-tests-to-detect-toxoplasma-gondii-serum-antibodies-in-farmed-red-deer
#1
Kandarp Khodidas Patel, Laryssa Howe, Cord Heuer, Geoffery William Asher, Peter Raymond Wilson
Abortion due to Toxoplasma gondii has been suspected in New Zealand farmed red deer. However, knowledge around the epidemiology and prevalence of T. gondii in farmed red deer is limited. The aim of this study was to firstly, assess the sensitivity and specificity of two commercially available assays, ELISA and latex agglutination test (LAT), for use in deer and secondly, to estimate the sero-prevalence of T. gondii in red deer. A total of 252 sera from rising 2-year-old and adult hinds from 17 New Zealand red deer herds at early and late pregnancy scanning and from known aborted and/or non-aborted hinds were tested for the presence of T...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28915725/distribution-of-rotavirus-genotypes-ccirculating-in-ahvaz-iran-in-2016
#2
Azarakhsh Azaran, Manoochehr Makvandi, Ali Teimoori, Saeedeh Ebrahimi, Farzad Heydari, Roya Nikfar
Background: Group A rotavirus (RVA) mainly causes acute gastroenteritis, exclusively in young children in developing countries. The prevalence and determination of the molecular epidemiology of rotavirus genotypes will determine the dominant rotavirus genotypes in the region and provide a strategy for the development of appropriate vaccines. Methods: A total of 100 fecal samples were collected from children below five years with acute gastroenteritis who referred to Aboozar Children’s Hospital of Ahvaz city during October 2015 to March 2016...
September 16, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28912421/pollination-of-ficus-elastica-india-rubber-re-establishes-sexual-reproduction-in-singapore
#3
Rhett D Harrison, Kwek Yan Chong, Nguyet Minh Pham, Alex T K Yee, Chow Khoon Yeo, Hugh T W Tan, Jean-Yves Rasplus
Ficus elastica, otherwise known as India Rubber (although its geographical origins are unclear), was an important source of latex in the early 19(th) century and was widely cultivated in tropical Asia. Like all figs, F. elastica is dependent on tiny, highly specific wasps for pollination, and detailed studies based out of Singapore in the 1930s suggested that through the loss of its pollinator F. elastica was extinct in the wild. However, around 2005 wild seedlings of F. elastica began appearing in Singapore...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28906587/vascularized-thumb-metacarpal-periosteal-pedicled-flap-for-scaphoid-nonunion-an-anatomical-study-and-pediatric-case-report
#4
Sergi Barrera-Ochoa, Gerardo Mendez-Sanchez, Alfonso Rodriguez-Baeza, Jorge Knörr, Jayme Augusto Bertelli, Francisco Soldado
PURPOSE: Through an anatomical review, the primary aim of this study was to delineate the dorsal thumb metacarpal (TM) periosteal branches of the radial artery (RA). In addition, we report here the clinical utility of a vascularized TM periosteal pedicled flap (VTMPF), supplied by the first dorsal metacarpal artery (FDMA), in a complex case of scaphoid nonunion. METHODS: Ten latex-colored upper limbs from fresh human cadavers were used. Branches of the RA were dissected under 3x loupe magnification, noting the periosteal branches arising from the FDMA...
September 14, 2017: Microsurgery
https://www.readbyqxmd.com/read/28906042/vascular-configurations-of-anastomotic-basket-of-conus-medullaris-in-human-spinal-cord
#5
Santiago Rojas, Marisa Ortega, Alfonso Rodríguez-Baeza
INTRODUCTION: The arterial basket of the conus medullaris is one of several anastomoses between the anterior and posterior spinal arteries. The anatomy of this structure has attracted little attention. This work sought to investigate its configuration in human spinal cords. MATERIALS AND METHODS: Spinal cords from male and female cadavers (n=32) were injected with colored latex through the intercostal, lumbar, medial sacral and the posterior trunks of the hypogastric arteries...
September 14, 2017: Clinical Anatomy
https://www.readbyqxmd.com/read/28905955/a-low-cost-large-field-of-view-scanning-ion-conductance-microscope-for-studying-nanoparticle-cell-membrane-interactions
#6
Astrid Gesper, Philipp Hagemann, Patrick Happel
Nanoparticles have the potential to become versatile tools in the medical and life sciences. One potential application is delivering drugs or other compounds to the cell cytoplasm, which requires the nanoparticles to bind to or cross the cell membrane. However, there are only a few tools available which allow studying the interaction of nanoparticles and the cell membrane of living cells in a physiological environment. Currently, the tool which least biases living cells is Scanning Ion Conductance Microscopy (SICM)...
September 14, 2017: Nanoscale
https://www.readbyqxmd.com/read/28900146/a-bead-assisted-flow-cytometry-method-for-the-semi-quantitative-analysis-of-extracellular-vesicles
#7
Henar Suárez, Ana Gámez-Valero, Raquel Reyes, Soraya López-Martín, María Josefa Rodríguez, José L Carrascosa, Carlos Cabañas, Francesc E Borràs, María Yáñez-Mó
Most experimental approaches commonly employed for the characterization and quantitation of EVs are time consuming, require of specialized instrumentation and often are rather inaccurate. To circumvent the caveats imposed by EV small size, we used general and specific membrane markers in bead assisted flow cytometry, to provide a semi-quantitative measure of EV content in a given sample. EVs were isolated from in vitro cultured cells-conditioned medium and biological fluids by size exclusion chromatography and coupled to latex beads to allow their detection by standard flow cytometers...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28899765/mechanical-particle-coating-using-polymethacrylate-nanoparticle-agglomerates-for-the-preparation-of-controlled-release-fine-particles-the-relationship-between-coating-performance-and-the-characteristics-of-various-polymethacrylates
#8
Keita Kondo, Shinsuke Kato, Toshiyuki Niwa
We aimed to understand the factors controlling mechanical particle coating using polymethacrylate. The relationship between coating performance and the characteristics of polymethacrylate powders was investigated. First, theophylline crystals were treated using a mechanical powder processor to obtain theophylline spheres (<100μm). Second, five polymethacrylate latexes were powdered by spray freeze drying to produce colloidal agglomerates. Finally, mechanical particle coating was performed by mixing theophylline spheres and polymethacrylate agglomerates using the processor...
September 9, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28895728/polycaprolactone-nanocomposites-reinforced-with-cellulose-nanocrystals-surface-modified-via-covalent-grafting-or-physisorption-a-comparative-study
#9
Assya Boujemaoui, Carmen Cobo Sanchez, Joakim Engström, Carl Bruce, Linda Fogelström, Anna Carlmark, Eva Malmström
In the present work, cellulose nanocrystals (CNCs) have been surface-modified either via covalent grafting or through physisorption of poly(n-butyl methacrylate) (PBMA) and employed as reinforcement in PCL. Covalent grafting was achieved by surface-initiated atom transfer radical polymerization (SI-ATRP). Two approaches were utilized for the physisorption; either using micelles of poly(dimethyl aminoethylmethacrylate)-block-poly(n-butyl methacrylate) (PDMAEMA-b-PBMA) or latex nanoparticles of poly(dimethyl aminoethylmethacrylate-co-methacrylic acid)-block-poly(n-butyl methacrylate) (P(DMAEMA-co-MAA)-b-PBMA)...
September 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28893002/interventional-angiography-radiation-protection-for-the-examiner-by-using-lead-free-gloves
#10
Peter Kamusella, Fabian Scheer, Christopher Wilhelm Lüdtke, Philipp Wiggermann, Christian Wissgott, Reimer Andresen
INTRODUCTION: The radiation exposure to unprotected parts of the body requires special attention for the interventional radiologist. During angiographic procedures, hands are exposed to the direct X-ray beam and scattered radiation. AIM: The aim of the study was to evaluate the radiation exposure of examiners hand with the use of lead-free X-ray protective gloves in clinical practice in terms of shielding and sense of touch. The aim of the study was to evaluate the radiation exposure of examiners hand with the use of lead-free X-ray protective gloves in clinical practice in terms of shielding and sense of touch...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28892873/combination-of-pcr-and-dual-nanoparticles-for-detection-of-plasmodium-falciparum
#11
Tienrat Tangchaikeeree, Duangporn Polpanich, Abderrazzak Bentaher, Abdoullatif Baraket, Abdelhamid Errachid, Geraldine Agusti, Abdelhamid Elaissari, Kulachart Jangpatarapongsa
Highly reactive particle-based DNA amplification was developed for the detection of the Pfg377 gene from P. falciparum gametocytes using functional magnetic latex particles (MLPs) and quantum dots encapsulated polymer particles (QDs-PPs). Firstly, MLPs were prepared from the precipitation of iron oxide, polymerization using initiator, and adsorption of aminodextran (AMD) so as to provide amino-functionalized MLPs. Furthermore, amino-containing polymer particles (PPs) were prepared by emulsifier-free polymerization and encapsulated with fluorescent quantum dots (QDs) for use as a signaling support...
September 2, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28891077/a-comparison-of-the-effects-of-cyp3a5-polymorphism-on-tacrolimus-blood-concentrations-measured-by-4-immunoassay-methods-in-renal-transplant-patients
#12
Yumiko Akamine, Hideaki Kagaya, Tadashi Ohkubo, Shigeru Satoh, Masatomo Miura
WHAT IS KNOWN AND OBJECTIVE: The anti-tacrolimus antibodies used in commercial immunoassay methods have cross-reactivity with tacrolimus metabolites. The aim of this study was to investigate differences in the effects of CYP3A5 polymorphism on tacrolimus concentrations obtained by four immunoassay methods in renal transplant patients. METHODS: Samples (n = 508) were evaluated using four immunoassays (chemiluminescence enzyme immunoassay [CLIA], affinity column-mediated immunoassay [ACMIA], electrochemiluminescence immunoassay [ECLIA] and latex agglutination turbidimetric immunoassay [LTIA])...
September 10, 2017: Journal of Clinical Pharmacy and Therapeutics
https://www.readbyqxmd.com/read/28890861/idiopathic-anaphylaxis
#13
REVIEW
Bright I Nwaru, Sangeeta Dhami, Aziz Sheikh
Idiopathic anaphylaxis is a rare life-threatening disorder with symptoms similar to other forms of anaphylaxis. There is lack of a robust evidence base underpinning the treatment of anaphylaxis and even less so for idiopathic anaphylaxis. Much of the evidence therefore comes from relatively small case series and expert opinion. Idiopathic anaphylaxis is a diagnosis of exclusion, requiring a thorough history and careful diagnostic work-up investigating possible triggers and underlying predisposing factors. Key diagnostic tests include skin-prick testing, tests for specific-IgE, component-resolved diagnostics, and in some cases for allergen challenge tests...
2017: Current Treatment Options in Allergy
https://www.readbyqxmd.com/read/28888250/latex-allergy-where-are-we-now-and-how-did-we-get-there
#14
Kevin J Kelly, Gordon Sussman
Latex allergy emerged as an epidemic of anaphylaxis, occupational asthma, and clinical dilemmas in the 1980s. A systematic recognition, investigation, discovery, epidemiology, and prevention strategy followed. International attention and collaborations of investigators, government agencies, manufacturing, and health policy resulted in near elimination of a global epidemic. This article summarizes nearly 4 decades of work in control of this epidemic and focuses attention on future problems that still require resolution...
September 2017: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/28886036/potential-role-of-sirtuin-1-in-m%C3%A3-ller-glial-cells-in-mice-choroidal-neovascularization
#15
Tomoka Ishida, Takeshi Yoshida, Kosei Shinohara, Kejia Cao, Ken-Ichi Nakahama, Ikuo Morita, Kyoko Ohno-Matsui
This study investigated the potential role of sirtuin 1 in Müller glial cells in choroidal neovascularization. In the in vitro study, primary Müller glial cells were cultured and treated with resveratrol, a sirtuin 1 activator. Glial fibrillary acidic protein expression and angiogenesis-related gene expression were examined using quantitative polymerase chain reaction and phagocytosis, as a marker of Müller glial cell function; in addition, a latex bead assay was used to analyze cell function. For the in vivo study, choroidal neovascularization was induced in C57BL/6 mice via laser photocoagulation, and resveratrol was administered intravitreally...
2017: PloS One
https://www.readbyqxmd.com/read/28885117/the-interperiosteodural-concept-applied-to-the-jugular-foramen-and-its-compartmentalization
#16
Florian Bernard, Ilyess Zemmoura, Jean Philippe Cottier, Henri-Dominique Fournier, Louis-Marie Terrier, Stéphane Velut
OBJECTIVE The dura mater is made of 2 layers: the endosteal layer (outer layer), which is firmly attached to the bone, and the meningeal layer (inner layer), which directly covers the brain and spinal cord. These 2 dural layers join together in most parts of the skull base and cranial convexity, and separate into the orbital and perisellar compartments or into the spinal epidural space to form the extradural neural axis compartment (EDNAC). The EDNAC contains fat and/or venous blood. The aim of this dissection study was to anatomically verify the concept of the EDNAC by focusing on the dural layers surrounding the jugular foramen area...
September 8, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/28882491/anatomic-and-high-resolution-computed-tomographic-angiography-study-of-the-lateral-femoral-condyle-flap-implications-for-surgical-dissection
#17
Mohamed Morsy, Yoo Joon Sur, Osman Akdag, Amr Eisa, Tarek A El-Gammal, Nirusha Lachman, Steven L Moran
BACKGROUND: The lateral femoral condyle (LFC) flap is a vascularized bone flap based on the superior lateral genicular artery (SLGA). Harvest technique for this flap has not yet been demonstrated. The purpose of this study was to better delineate the blood supply to the bone and skin of this flap to allow for a safe and effective harvest. MATERIALS AND METHODS: Twenty-three lower extremities were injected with latex or a mixture of latex and barium sulfate. The SLGA was identified and dissected, documenting the course, diameter, anatomical relations, length, and branches...
August 16, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/28878804/the-latex-protein-mlx56-from-mulberry-morus-multicaulis-protects-plants-against-insect-pests-and-pathogens
#18
Ying-Ping Gai, Ya-Nan Zhao, Huai-Ning Zhao, Chuan-Zhong Yuan, Shuo-Shuo Yuan, Shuo Li, Bing-Sen Zhu, Xian-Ling Ji
Biotic stresses are major constraints limiting the leaf quality and productivity of mulberry. MLX56 is a unique chitin-binding protein isolated from Shin-Ichinose (Morus alba) latex that displays toxicity against lepidopteran caterpillars. In this study, the full-length cDNA encoding MLX56 was isolated from Husang 32 (M. multicaulis) and designated HMLX56. Amino acid sequence analysis and protein modeling of three MLX56 proteins showed that they were highly conserved among Morus species. Tissue expression pattern analysis showed that the HMLX56 gene was strongly expressed in mulberry bark and leaves but only slightly expressed in fruits...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28877760/sbmlpkgspec-a-latex-style-file-for-sbml-package-specification-documents
#19
Michael Hucka
OBJECTIVE: The Systems Biology Markup Language (SBML) is a popular open format for storing and exchanging computational models in biology. The definition of SBML is captured in formal specification documents. SBMLPkgSpec is a LaTeX document style intended to fill the need for a standard format for writing such specification documents. RESULTS: Specification documents for SBML Level 3 extensions (known as packages in SBML) are made more uniform with the use of a standard template...
September 6, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28877490/adaptive-response-of-actin-bundles-under-mechanical-stress
#20
Florian Rückerl, Martin Lenz, Timo Betz, John Manzi, Jean-Louis Martiel, Mahassine Safouane, Rajaa Paterski-Boujemaa, Laurent Blanchoin, Cécile Sykes
Actin is one of the main components of the architecture of cells. Actin filaments form different polymer networks with versatile mechanical properties that depend on their spatial organization and the presence of cross-linkers. Here, we investigate the mechanical properties of actin bundles in the absence of cross-linkers. Bundles are polymerized from the surface of mDia1-coated latex beads, and deformed by manipulating both ends through attached beads held by optical tweezers, allowing us to record the applied force...
September 5, 2017: Biophysical Journal
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