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https://www.readbyqxmd.com/read/28104390/progesterone-and-vip-cross-talk-enhances-phagocytosis-and-anti-inflammatory-profile-in-trophoblast-derived-cells
#1
Daiana Vota, Mariana Aguero, Esteban Grasso, Vanesa Hauk, Lucila Gallino, Elizabeth Soczewski, Claudia Pérez Leirós, Rosanna Ramhorst
Trophoblast cells produce several inmmuneregulators like the Vasoactive Intestinal Peptide (VIP) and P4 targeting multiple circuits, and also display an intese phagocytic ability allowing embryo implantation in a tolerogenic context. Here, we explored whether P4 and VIP- crosstalk modulates trophoblast cell function, focus on the phagocytic ability and the immune homeostasis maintenance. P4 enhanced the phagocytosis in trophoblast-derived cells quantified by the engulfment of latex-beads or eryptotic erythrocytes...
January 16, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28101016/cellulitis-in-a-liver-transplant-patient-as-an-initial-manifestation-of-disseminated-cryptococcal-disease
#2
Marcos Davi Gomes de Sousa, Fred Bernardes Filho, Luís Eduardo Barros Costa Fernandes, Cássia Regina Guedes Leal, Cristiane Rocha Magalhães, Marcos Antonio Custódio Neto da Silva, Halime Silva Barcaui
A 50-year-old male underwent liver transplantation due to cryptogenic cirrhosis and was admitted with severe pain in the left leg as well as phlogosis. Amoxicillin/clavulanic acid was prescribed, assuming bullous erysipelas. Among the tests performed, the latex agglutination test for the Cryptococcus sp. antigen was positive, and in both the blood culture and blister culture Cryptococcus sp. was isolated. Daily fluconazole was started. Even though liposomal amphotericin B has been started on the fifth day of hospitalization, the patient progressed to death...
September 2016: Case Reports in Dermatology
https://www.readbyqxmd.com/read/28099000/application-of-nano-fe%C3%A2-tannic-acid-complexes-in-modifying-aqueous-acrylic-latex-for-controlled-release-coated-urea
#3
Yazhen Shen, Changwen Du, Jianmin Zhou, Fei Ma
Acrylic latexes are valuable waterborne materials used in controlled-release fertilizers. Controlled-release urea coated with these latexes release a large amount of nutrients, making it difficult to meet the requirement of plants. Herein, FeⅢ-Tannic Acid (TA) complexes, which were blended with acrylic latex and subsequently reassembled capsules on a surface of polyacrylate particles. These complexes remarkably retarded the release of urea (the preliminary solubility was decreased from 22.3% to 0.8%) via decreasing the coating tackiness (Tg was increased from 4...
January 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28093371/sonographically-guided-knee-meniscus-injections-feasibility-techniques-and-validation
#4
Michael R Baria, Jacob L Sellon, Dan Lueders, Jay Smith
BACKGROUND: There is a growing interest in the use of biologic agents such as platelet-rich plasma and mesenchymal stem/stromal cells to treat musculoskeletal injuries, including meniscal tears. Although prior research has documented the role of diagnostic ultrasound to evaluate meniscal tears, sonographically guided (SG) techniques to specifically deliver therapeutic agents into the meniscus have not been described. OBJECTIVE: To describe and validate SG injection techniques for the body and posterior horn of the medial and lateral meniscus...
January 13, 2017: PM & R: the Journal of Injury, Function, and Rehabilitation
https://www.readbyqxmd.com/read/28081456/robust-tough-and-anti-fatigue-cationic-latex-composite-hydrogels-based-on-dual-physically-cross-linked-networks
#5
Song Gu, Lijie Duan, Xiuyan Ren, Guang Hui Gao
Dual physically cross-linked hydrogels, which are triggered by cationic latexes as hydrophobic association and ionic crosslinking centers, were easily fabricated via a one-pot in situ polymerization method. First, the hydrophobic alkyl chains of hydrophobic monomers are adsorbed on the surface of latex microspheres and stabilized in the presence of surfactants, forming hydrophobic association centers as the first physical crosslinking points. Meanwhile, the anionic sulfate radicals dissociated by persulphate are attracted towards the cationic molecular chains of latex microspheres through ionic interactions, forming the secondary physical crosslinking centers, and initiate the copolymerization between acrylamide and hydrophobic vinyl monomers...
January 4, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28078650/in-vivo-efficacy-of-latex-from-calotropis-procera-in-ameliorating-fever-biochemical-characteristics-and-plausible-mechanism
#6
Vijay L Kumar, B Guruprasad, Syed Meraj A Fatmi, Priyanka Chaudhary, Nylane Maria Nunes Alencar, José Vitor Moreira Lima-Filho, Márcio Viana Ramos
Calotropis procera latex fractions possessing anti-inflammatory property were characterized for their biochemical properties, compared for their efficacy in ameliorating fever in rats and their mechanism of action was elucidated. Aqueous fraction and methanol extract (AqDL and MeDL) were derived from the dried latex (DL) and proteins were separated from the fresh latex (LP). Polyacrylamide gel electrophoresis carried out under denaturing conditions showed the presence of proteins with some similarity in LP and AqDL and both of these fractions exhibited proteinase activity by gelatin zymography...
January 11, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28078336/design-of-latex-layered-double-hydroxide-composites-by-tuning-the-aggregation-in-suspensions
#7
Marko Pavlovic, Paul Rouster, Elodie Bourgeat-Lami, Vanessa Prevot, Istvan Szilagyi
Colloidal stability of polymeric latex particles was studied in the presence of oppositely charged layered double hydroxide (LDH) platelets of different interlayer anions. Adsorption of the LDH particles led to charge neutralization and to overcharging of the latex at appropriate concentrations. Mixing stable colloidal suspensions of individual particles results in rapid aggregation once the LDH adsorption neutralizes the negative charges of the polymer spheres, while stable suspensions were observed at high and low LDH doses...
January 12, 2017: Soft Matter
https://www.readbyqxmd.com/read/28068859/isolation-and-characterisation-of-cytotoxic-compounds-from-euphorbia-clementei-boiss
#8
Abbes Benmerache, Abdulmagid Alabdul Magid, Amira Labed, Ahmed Kabouche, Laurence Voutquenne-Nazabadioko, Jane Hubert, Hamid Morjani, Zahia Kabouche
A new phorbol-type diterpene ester, 4,20-dideoxy-4α-phorbol-12β-acetate-13α-isobutyrate, in addition to 11 known compounds were isolated from the latex and roots of Euphorbia clementei Boiss. Structure elucidation was performed by comprehensive 1D and 2D NMR analyses ((1)H and (13)C NMR, COSY, ROESY, HSQC and HMBC experiments), mass spectrometry (HR-ESI-MS) and by comparison with literature data. The inhibitory activity of all isolated compounds was evaluated against promyelocytic leukemia HL60 and human erythromyeloblastoid leukemia K562 cell lines and seven of these compounds exhibited a weak cytotoxicity with IC50 values ranging from 40 to 97 μM...
January 9, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28068637/assembling-of-multifunctional-latex-based-hybrid-nanocarriers-from-calotropis-gigantea-for-sustained-doxorubicin-dox-releases
#9
Periyakaruppan Pradeepkumar, Dharman Govindaraj, Murugaraj Jeyaraj, Murugan A Munusamy, Mariappan Rajan
Natural rubber Latex (Lax) is a colloidal dispersion of polymer particles in liquid and shows good biodegradable, biocompatibility, and non-toxicity. Natural polymers are the most important materials used in food packaging, micro/nano-drug delivery, tissue engineering, agriculture, and coating. In the present study, natural compounds extracted from plant Lax were designed to function as drug carriers using various surfactants via emulation and solvent evaporation method. Calotropis gigantea belongs to the family Apocynaceae and has received considerable attention in modern medicine, ayurvedeic, siddha, and traditional medicine...
January 6, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28067755/vascular-structures-of-the-lateral-wall-of-the-maxillary-sinus-a-vascular-labeling-technique
#10
José Caraballo, Alba Gálvez, María Cristina Manzanares-Céspedes, Iván Valdivia-Gandur, Rui Figueiredo, Eduard Valmaseda-Castellón
PURPOSE: Sinus floor augmentation is a common procedure in implant dentistry. However, several intraoperative complications can occur during this procedure, such as bleeding from the lateral wall of the maxillary sinus. The aim of this study was to describe the vascular structures of the lateral wall of the maxillary sinus using a vascular labeling technique. MATERIALS AND METHODS: Ten cadaveric specimens were prepared by the vascular labeling technique. Liquid latex was injected into the large vessels of the head, and the lateral wall of the maxillary sinus was exposed by dissection...
January 6, 2017: Implant Dentistry
https://www.readbyqxmd.com/read/28065456/characterization-and-functional-expression-of-a-rubber-degradation-gene-of-a-nocardia-degrader-from-a-rubber-processing-factory
#11
Dao Viet Linh, Nguyen Lan Huong, Michiro Tabata, Shunsuke Imai, Sou Iijima, Daisuke Kasai, To Kim Anh, Masao Fukuda
A rubber-degrading bacterial consortium named H2DA was obtained from an enrichment culture with natural rubber latex and rubber-processing factory waste in Vietnam. Gel permeation chromatography analysis revealed that only the strain NVL3 degraded synthetic poly(cis-1,4-isoprene) into low-molecular-weight intermediates among the three strains found in the H2DA. The 16S-rRNA gene sequence of NVL3 showed the highest identity with that of Nocardia farcinica DSM 43665(T). NVL3 accumulated aldehyde intermediates from synthetic poly(cis-1,4-isoprene) on a rubber-overlay plate as indicated by Schiff's staining...
January 3, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28062071/chemical-profile-and-defensive-function-of-the-latex-of-euphorbia-peplus
#12
Juan Hua, Yan Liu, Chao-Jiang Xiao, Shu-Xi Jing, Shi-Hong Luo, Sheng-Hong Li
Plant latex is an endogenous fluid secreted from highly specialized laticifer cells and has been suggested to act as a plant defense system. The chemical profile of the latex of Euphorbia peplus was investigated. A total of 13 terpenoids including two previously unknown diterpenoids, (2S*,3S*,4R*,5R*,6R*,8R*,l1R*,13S*,14S*,15R*, 16R*)-5,8,15-triacetoxy-3-benzoyloxy-11,16-dihydroxy-9-oxopepluane and (2R*,3R*, 4S*,5R*,7S*,8S*,9S*,l3S*,14S*,15R*)-2,5,8,9,14-pentaacetoxy-3-benzoyloxy-15-hydroxy-7-isobutyroyloxyjatropha-6(17),11E-diene), ten known diterpenoids, and a known acyclic triterpene alcohol peplusol, were identified, using HPLC and UPLC-MS/MS analyses and through comparison with the authentic compounds isolated from the whole plant...
January 3, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28057569/a-novel-peroxidase-purified-from-marsdenia-megalantha-latex-inhibits-phytopathogenic-fungi-mediated-by-cell-membrane-permeabilization
#13
Henrique P Oliveira, Rodolpho G G Silva, Jose T A Oliveira, Daniele O B Sousa, Mirella L Pereira, Pedro F N Souza, Arlete A Soares, Valdirene M Gomes, Ana C O Monteiro-Moreira, Frederico B M B Moreno, Ilka M Vasconcelos
An antifungal class III peroxidase was purified from Marsdenia megalantha latex (named Mo-POX) using DEAE-cellulose and gel filtration chromatography on a Superose 12 HR 10/30 column. Mm-POX has an apparent molecular mass of 67.0kDa and a pI of 5.2, shares identity with other peroxidases, and follows Michaelis-Menten kinetics. It has a high affinity for guaiacol and hydrogen peroxide. The pH and temperature optima for Mm-POX were 5.0-7.0 and 60°C, respectively. The catalytic activity of Mm-POX was decreased in the presence of classic peroxidase inhibitors including azide, dithiothreitol, ethylenediamine tetraacetic acid, and sodium metabisulfite and high concentrations of Na(+), Mn(+), and salicylic acid...
January 3, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28057488/exploring-hemostatic-and-thrombolytic-potential-of-heynein-a-cysteine-protease-from-ervatamia-heyneana-latex
#14
Priyanka Uday, M Maheshwari, P Sharanappa, Zohara Nafeesa, Vivek Hamse Kameshwar, B S Priya, S Nanjunda Swamy
ETHNOPHARMACOLOGIAL RELEVANCE: The latex of Ervatamia heyneana (Wall.) T. Cooke plant has been used for wound healing and various skin diseases by Indian tribes and folklore. AIM OF THE STUDY: To validate the scientific basis of heynein - a key protease of Ervatamia heyneana, in hemostasis and wound healing process. MATERIALS AND METHODS: The latex from E. heyneana was processed and subjected to two step purification. The purified heynein was assayed for proteolytic activity using casein as substrate and also attested by zymography...
January 2, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28054632/a-sensitive-three-monoclonal-antibodies-based-automatic-latex-particle-enhanced-turbidimetric-immunoassay-for-golgi-protein-73-detection
#15
Yanyan Xia, Han Shen, Yefei Zhu, Hongpan Xu, Zhiyang Li, Jin Si
Golgi protein 73 (GP73) is a novel and potential marker for diagnosing hepatocellular carcinoma (HCC) that has been found to be abnormally elevated in liver disease. A latex particle-enhanced turbidimetric immunoassay (LTIA) was recently introduced and licensed for application in a variety of automated clinical chemistry analyzers. However, no studies have reported sufficient data on analytical performance of this method when using 3 monoclonal antibodies for GP73 measurement. The experimental conditions were firstly optimized and range of linearity, diagnostic potential, clinical relevance were compared with the LTIA based on polyclonal antibodies and ELISA...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28054515/method-validation-of-nanoparticle-tracking-analysis-to-measure-pulmonary-nanoparticle-content-the-size-distribution-in-exhaled-breath-condensate-depends-on-occupational-exposure
#16
Jean-Jacques Sauvain, Guillaume Suarez, Jean-Louis Edmé, Olivia Maria de Paula Alvez Bezerra, Keller Silveira, Lenio Amaral, Ana Paula Carneiro, Natalie Cherot-Kornobis, Annie Sobaszek, Sébastien Hulo
A particle exposure assessment based on the dose deposited in the lungs would be the gold standard for the evaluation of any resulting health effects. Measuring particles in exhaled breath condensate (EBC)-a matrix containing water and airway lining fluid-could help to evaluate particle retention in the lungs. This study aimed to (1) validate a nanoparticle tracking analysis (NTA) method for determining the particle number concentration and their hydrodynamic size distribution in EBC, and (2) apply this method to EBC collected from workers exposed to soapstone (n = 55) or quartz dust (n = 12) and controls (n = 11)...
January 5, 2017: Journal of Breath Research
https://www.readbyqxmd.com/read/28052674/dye-aggregates-as-new-stabilizers-for-mini-emulsions
#17
Susanne Sihler, Ulrich Ziener
Water-soluble organic dyes like fluorescein are widely used, mainly for coloration of e.g. biological samples, groundwater tracing etc., and they are not obviously amphiphilic by molecular structure like surfactants. Here, we show that the dyes alone stabilize oil-in-water emulsions. Exemplarily, fluorescein is compared with the classical surfactant sodium dodecyl sulfate (SDS) by means of surface/interfacial tension, concentration of stabilizer and electrolyte as well as pH. The principle can be extended to further classes of water-soluble dyes, which keep up with or exceed SDS by efficiency...
January 4, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28052415/arthroscopic-approach-and-intra-articular-anatomy-of-the-dorsal-and-plantar-synovial-compartments-of-the-bovine-tarsocrural-joint
#18
Hélène Lardé, Sylvain Nichols, Marie Babkine, André Desrochers
OBJECTIVE: To determine arthroscopic approaches to the dorsal and plantar synovial compartments of the tarsocrural joint in adult cattle, and to describe the arthroscopic intra-articular anatomy from each approach. STUDY DESIGN: Ex vivo study. ANIMALS: Fresh adult bovine cadavers (n = 7). METHODS: Two tarsocrural joint were injected with latex to determine arthroscopic portal locations and arthroscopy of the tarsocrural joint of 12 tarsi was performed...
January 2017: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/28051098/production-of-itaconate-by-whole-cell-bioconversion-of-citrate-mediated-by-expression-of-multiple-cis-aconitate-decarboxylase-cada-genes-in-escherichia-coli
#19
Junyoung Kim, Hyung-Min Seo, Shashi Kant Bhatia, Hun-Seok Song, Jung-Ho Kim, Jong-Min Jeon, Kwon-Young Choi, Wooseong Kim, Jeong-Jun Yoon, Yun-Gon Kim, Yung-Hun Yang
Itaconate, a C5 unsaturated dicarboxylic acid, is an important chemical building block that is used in manufacturing high-value products, such as latex and superabsorbent polymers. Itaconate is produced by fermentation of sugars by the filamentous fungus Aspergillus terreus. However, fermentation by A. terreus involves a long fermentation period and the formation of various byproducts, resulting in high production costs. E. coli has been developed as an alternative for producing itaconate. However, fermentation of glucose gives low conversion yields and low productivity...
January 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28048706/tu-ab-bra-06-best-in-physics-joint-imaging-therapy-an-mri-compatible-externally-and-internally-deformable-lung-motion-phantom-for-multi-modality-igrt
#20
P Sabouri, T Arai, A Sawant
PURPOSE: MRI has become an attractive tool for tumor motion management. Current MR-compatible phantoms are only capable of reproducing translational motion. This study describes the construction and validation of a more realistic, MRI-compatible lung phantom that is deformable internally as well as externally. We demonstrate a radiotherapy application of this phantom by validating the geometric accuracy of the open-source deformable image registration software NiftyReg (UCL, UK). METHODS: The outer shell of a commercially-available dynamic breathing torso phantom was filled with natural latex foam with eleven water tubes...
June 2016: Medical Physics
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