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Hao Li, Xian-Mei Tang, Yangchen Liu, Weizhen Li, Qiaoyun Chen, Yan Pan
BACKGROUND/AIMS: The HOX transcript antisense intergenic RNA (HOTAIR), a long non-coding RNA (lncRNA), plays an important role in the pathogenesis and progression of multiple tumors. The aim of the present study was to evaluate whether common single nucleotide polymorphisms (SNPs) in HOTAIR are related to hepatocellular carcinoma (HCC) susceptibility in a Chinese population. METHODS: We genotyped three SNPs of HOTAIR in a hepatocellular carcinoma (HCC) case-control study, including 482 cases and 520 control subjects...
November 15, 2017: Cellular Physiology and Biochemistry
Xinyang Lu, Zhiqiang Liu, Xiaofei Ning, Lunhua Huang, Biao Jiang
The long non-coding RNA HOX transcript antisense RNA (HOTAIR) has been found overexpressed in many human malignancies and involved in tumor progression and metastasis. Although the downstream target through which HOTAIR modulates tumor metastasis is not well-known, evidence suggests that microRNA-197 (miR-197) might be involved in this event. In the present study, the significance of HOTAIR and miR-197 in the progression of colorectal cancer were detected in vitro and in vivo. We found that HOTAIR expression was significantly increased in colorectal cancer cells and tissues...
November 14, 2017: Oncology Research
Naomi Li, Steven Hébert, Jingwen Song, Claudia L Kleinman, Stéphane Richard
The KH-type RNA binding protein Sam68 is required for adipogenesis. We have previously shown that Sam68-deficient mice have a lean phenotype and are protected against dietary-induced obesity due to defects in mTOR and S6K1 alternative splicing. Herein we profiled the transcriptome of Sam68 wild type and deficient 3T3-L1 mouse preadipocytes. We identified 652 protein-coding genes and 9 ncRNAs that were significantly altered with the loss of Sam68. As expected, downregulated genes were significantly associated with GO terms linked to cell migration, motility, and fat cell differentiation, while upregulated genes were mostly associated with GO terms linked to neurogenesis...
October 10, 2017: Oncotarget
Min Zhang, Yinghui Song, Furui Zhai
BACKGROUND: The objective of this study was to explore the role of HOTAIR in the development of cervical cancer, as well as its downstream signaling pathway. METHOD: We conducted computational analysis, luciferase assay to explore downstream of HOTAIR and miR-331-3p. Real-time PCR and western-blot were carried out to detect the relationship among E7, HOTAIR, miR-331-3p, NRP2 and P53. Finally, MTT assay and flow cytometry analysis were performed to validate the effect of E7 and miR-331-3p on cell apoptosis and proliferation...
November 11, 2017: Journal of Cellular Biochemistry
Da-Ni Zhong, Yi-Huan Luo, Wei-Jia Mo, Xin Zhang, Zhong Tan, Na Zhao, Si-Min Pang, Gang Chen, Min-Hua Rong, Wei Tang
HOX transcript antisense RNA (HOTAIR), a newly discovered long noncoding RNA (lncRNA), has been reported to be a poor prognostic marker in many types of cancers. The current study attempted to investigate the biological roles and clinicopathlogical implications of HOTAIR in hepatocellular carcinoma (HCC), as well as understand the molecular mechanisms of HOTAIR in HCC progression. HOTAIR expression in 95 HCC patients with paired HCC tissues and adjacent non‑cancer tissues were investigated using quantitative reverse transcription‑polymerase chain reaction...
November 7, 2017: Molecular Medicine Reports
Zheng Li, Xingye Li, Chao Jiang, Wenwei Qian, Gary Tse, Matthew T V Chan, William K K Wu
Rheumatoid arthritis, a disabling autoimmune disease, is associated with altered gene expression in circulating immune cells and synovial tissues. Accumulating evidence has suggested that long non-coding RNAs (lncRNAs), which modulate gene expression through multiple mechanisms, are important molecules involved in immune and inflammatory pathways. Importantly, many studies have reported that lncRNAs can be utilized as biomarkers for disease diagnosis and prognostication. Recently, dysregulation of lncRNAs in rheumatoid arthritis and other autoimmune diseases has been revealed...
November 7, 2017: Cell Proliferation
Shuqi Chi, Lina Shen, Teng Hua, Shuangge Liu, Guobing Zhuang, Xiaoxiao Wang, Xing Zhou, Guozhen Wang, Hongbo Wang
Long noncoding RNAs (lncRNAs) have been reported to be abnormally expressed in cervical cancer (CC) and presumably serve as diagnostic or prognostic markers. We thus performed a systematic review and meta-analysis to evaluate the clinical values of dysregulated lncRNAs in CC. A literature search was performed using the electronic databases PubMed, Embase, and Web of Science. A total of 22 relevant studies were eligible, including 21 on clinicopathological features, 18 on prognosis, and 4 on diagnosis. For clinicopathological features, HOTAIR expression was positively associated with tumor size (odds ratio [OR]=2...
October 3, 2017: Oncotarget
Danju Kang, Guangxian Zhou, Shiwei Zhou, Jie Zeng, Xiaolong Wang, Yu Jiang, Yuxin Yang, Yulin Chen
Adipose tissues are phenotypically, metabolically and functionally heterogeneous based on the sites of their deposition. Undesirable fat deposits in the body are often detrimental to animal and human health. To unravel the potential underlying mechanisms governing accumulation of adipose tissues in various regions of the body, i.e., subcutaneous (SAT), visceral (VAT) and tail (TAT), we profiled transcriptomes from Tan sheep, a Chinese indigenous breed with notable fat tail using RNA-seq. Upon comparison, we identified a total of 1,058 differentially expressed genes (DEGs) between the three adipose types (218, 324, and 795 in SAT/VAT, SAT/TAT, and VAT/TAT, respectively), from which several known key players were identified that are involved in lipid metabolic process, Wnt signals, Vitamin A metabolism, and transcriptional regulation of adipocyte differentiation...
November 3, 2017: Scientific Reports
Wei Liu, Nan-Chang Yin, Hao Liu, Ke-Jun Nan
OBJECTIVES: Lung cancer is one of the most common malignant tumors worldwide, and its morbidity and mortality rates continue to rise. The role of lncRNAs in lung cancer has become an emerging area of research. MATERIALS AND METHODS: The expression of CAV-1 and HOTAIR was determined in lung cancer tissues and cell lines by western blot and RT-qPCR. Cell proliferation, migration and invasion were analysed in lung cell following knockdown or overexpression of CAV-1 or HOTAIR by transfection with small interfering RNA (siRNA) or plasmid...
October 25, 2017: Gene
Chuanyao Cheng, Yaguang Qin, Qiongjie Zhi, Jianjun Wang, Changjiang Qin
HOX transcript antisense RNA (HOTAIR), a well-known long non-coding RNA (lncRNA), has been reported to be related to cisplatin (DDP) resistance in gastric cancers. However, the molecular mechanism of HOTAIR in DDP resistance of gastric cancer (GC) remains largely undefined. The aim of this study was to investigate the role and underlying mechanism of HOTAIR in regulating cisplatin resistance of GC. The results showed that HOTAIR was over-expressed in GC tissues and GC cell lines, while miR-34a was low-expressed...
October 26, 2017: International Journal of Biological Macromolecules
Ying Wu, Qian Xiong, Siting Li, Xue Yang, Feng Ge
Long non-coding RNA HOX transcript antisense RNA (HOTAIR) is involved in human tumorigenesis and is dysregulated in hepatocellular carcinoma (HCC). However, the molecular mechanisms underlying HOTAIR functions in HCC are largely unknown. Here, we employed an integrated transcriptomic and quantitative proteomic analysis to systematically explore the regulatory role of HOTAIR in HCC. A total of 673 transcripts and 293 proteins were found to be dysregulated after HOTAIR inhibition. Bioinformatics studies indicated that differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) are involved in many biological processes, especially cancer-related signaling pathways...
October 27, 2017: Molecular & Cellular Proteomics: MCP
Giuseppe Troiano, Vito Carlo Alberto Caponio, Linda Boldrup, Xiaolian Gu, Lorenzo Lo Muzio, Nicola Sgaramella, Lixiao Wang, Karin Nylander
INTRODUCTION: Long noncoding RNAs (lncRNAs) are often dysregulated in cancer tissue and seem to play an important role in neoplastic processes. Recent studies have shown that the HOX transcript antisense intergenic RNA (HOTAIR) may play a role as a marker of prognosis in squamous cell carcinoma of the head and neck (SCCHN). The aim of this study was to perform a meta-analysis of studies focused on the prognostic role of HOTAIR in SCCHN. RESULTS: At the end of the selection process, four studies were considered eligible for inclusion in the meta-analysis, comprising a total of 271 patients...
September 22, 2017: Oncotarget
Ranran Wang, Bin Yan, Zheng Li, Yiqun Jiang, Chao Mao, Xiang Wang, Xinmin Zhou
Evidence suggests that both 14-3-3σ and long non-coding RNA HOX transcript antisense RNA (HOTAIR) are involved in the tumorigenesis and progression of lung cancer. In the present study, the potential association between 14-3-3σ and HOTAIR in non-small cell lung cancer (NSCLC) was investigated. In tissue samples collected from 54 patients with NSCLC, expression of HOTAIR and 14-3-3σ was analyzed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). After stable ectopic expression of HOTAIR and stable HOTAIR knockdown in PC9 cancer cells, the effect of HOTAIR on levels of mRNA and protein 14-3-3σ expression levels were detected using RT-qPCR and western blotting, respectively...
November 2017: Experimental and Therapeutic Medicine
Tek N Lamichhane, Christopher A Leung, Lampouguin Yenkoidiok Douti, Steven M Jay
Extracellular vesicles (EVs), such as exosomes, have been identified as regulators of vascular remodeling and have promise as therapeutics for vascularization applications. Towards development of EVs as therapeutics, it has been demonstrated that physiological stimuli of angiogenic phenotypes in EV-producing cells can enhance the potency of EVs for vascularization. The goal of this study was to assess whether ethanol, which induces angiogenic phenotypes in endothelial cells, could be employed to enhance endothelial-derived EV vascularization bioactivity...
October 23, 2017: Scientific Reports
Majid Pahlevan Kakhki, Abbas Nikravesh, Zeinab Shirvani Farsani, Mohammad Ali Sahraian, Mehrdad Behmanesh
Different studies have revealed that dysregulation in gene expression is one of the main aspects of multiple sclerosis (MS) pathogenesis. Although the molecular pathways underlying the immunomodulatory role of vitamin D (VD) in MS is not completely elucidated, VD has more recently become a topic of interest in immune regulation and is widely administrated to MS patients as an immunomodulatory supplement. Long non-coding RNAs (lncRNAs) are known to play important roles in regulation of gene expression via different mechanisms...
October 14, 2017: Immunology
Weiliang Sun, Yunben Yang, Chunjing Xu, Junming Guo
Long non-coding RNAs (lncRNAs) are a heterogeneous class of RNAs that are non-protein coding transcripts longer than 200 nucleotides. In this review, we introduce the mechanisms by which lncRNAs regulate gene expression in four parts, epigenetic regulation (genetic imprinting and chromatin remodeling), transcriptional regulation (molecular decoy), post-transcriptional regulation (splicing and mRNA decay), and translational regulation. H19, Xist, and others are involved in genomic imprinting. HOTAIR and ANRIL function in chromatin remodeling...
October 2017: Cancer Genetics
Shekoufeh Hassanzarei, Mohammad Hashemi, Hedieh Sattarifard, Seyed Mehdi Hashemi, Gholamreza Bahari, Saeid Ghavami
There is an increasing body of evidence which highlights the critical functions of long non-coding RNAs in the carcinogenicity mechanism of a variety of cancers. It has been reported that HOX transcript antisense intergenic RNA, a member of long non-coding RNA family, increases breast cancer risk. To date, no data regarding the association between HOX transcript antisense intergenic RNA polymorphisms and the risk of breast cancer development has been reported in Iran. Here, we examine the possible association between HOX transcript antisense intergenic RNA gene polymorphisms and breast cancer in a sample of southeast Iranian female population...
October 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Weiyi Pan, Chenzhou Wu, Zhifei Su, Zexi Duan, Longjiang Li, Fanglin Mi, Chunjie Li
Genetic polymorphisms, including single nucleotide polymorphisms (SNP) and nucleotide repeat expansions, can occur in regions that transcribe non-coding RNAs (ncRNA), such as, but not limited to, micro RNA and long non-coding RNA. An association between genetic polymorphisms of ncRNA and increasing head and neck cancer (HNC) risk has been identified by several studies. Therefore, the aim of this systematic review is to consolidate existing findings to clarify this association. Four electronic databases, such as MEDLINE, EMBASE, Chinese BioMedical Literature Database, and China National Knowledge Infrastructure, were utilised...
September 22, 2017: Oncotarget
Xiangrong Cui, Xuan Jing, Xueqing Wu
Cervical cancer is one of the most common malignancies in women worldwide. Numerous literatures demonstrate that aberrantly expressed lncRNAs are involved in tumorigenesis and development, and may have the potential to be prognostic markers. However, their prognostic functions in cervical remain controversial. Therefore, we performed this meta-analysis to evaluate the prognostic significance of lncRNAs in cervical cancer. We searched databases to identify relevant articles. Pooled hazard ratios (HRs) and 95% confidence intervals (CIs) were calculated...
September 22, 2017: Oncotarget
Wen-Ming Chen, Wei-Dong Chen, Xue-Mei Jiang, Xue-Feng Jia, Hong-Mei Wang, Qiu-Jie Zhang, Yong-Qian Shu, Hai-Bo Zhao
AIM: To clarify the mechanisms of HOX transcript antisense intergenic RNA (HOTAIR) in gastric cancer (GC) migration and invasion. METHODS: Quantitative real-time polymerase chain reaction (qPCR) was used to detect the expression level of HOTAIR in GC tissues. The correlation of its expression with clinicopathological features was analyzed. Area under receiver operating characteristic curve (AUCROC) was constructed to evaluate the diagnostic value of HOTAIR. Wound-healing assay and Transwell assay were performed to detect the biological effects of HOTAIR in GC cells...
September 7, 2017: World Journal of Gastroenterology: WJG
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