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https://www.readbyqxmd.com/read/28345995/description-and-comparison-of-excretory-urography-performed-during-radiography-and-computed-tomography-for-evaluation-of-the-urinary-system-in-healthy-new-zealand-white-rabbits-oryctolagus-cuniculus
#1
Laura Vilalta, Raul Altuzarra, Yvonne Espada, Elisabet Dominguez, Rosa Novellas, Jaime Martorell
OBJECTIVE To evaluate the usefulness of excretory urography performed during radiography (REU) and CT (CTEU) in healthy rabbits, determine timings of urogram phases, and compare sensitivities of REU and CTEU for detection of these phases. ANIMALS 13 New Zealand White rabbits (Oryctolagus cuniculus). PROCEDURES Rabbits were screened for signs of systemic and urinary tract disease. An REU examination of each was performed, followed ≥ 5 days later by a CTEU examination. Contrast images from each modality were evaluated for quality of opacification and intervals between initiation of contrast medium administration and detection of various urogram phases...
April 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28344104/antimicrobial-medium-and-long-chain-free-fatty-acids-prevent-prfa-dependent-activation-of-virulence-genes-in-listeria-monocytogenes
#2
Eva Maria Sternkopf Lillebæk, Stine Lambert Nielsen, Rikke Scheel Thomasen, Nils J Færgeman, Birgitte H Kallipolitis
The foodborne pathogen Listeria monocytogenes is the causative agent of the invasive disease listeriosis. Infection by L. monocytogenes involves bacterial crossing of the intestinal barrier and intracellular replication in a variety of host cells. The PrfA protein is the master regulator of virulence factors required for bacterial entry, intracellular replication and cell-to-cell spread. PrfA-dependent activation of virulence genes occurs primarily in the blood and during intracellular infection. In contrast, PrfA does not play a significant role in regulation of virulence gene expression in the intestinal environment...
March 23, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28343387/hydroxyl-and-methoxyl-derivatives-of-benzylglucosinolate-in-lepidium-densiflorum-with-hydrolysis-to-isothiocyanates-and-non-isothiocyanate-products-substitution-governs-product-type-and-mass-spectral-fragmentation
#3
Eleonora Pagnotta, Niels Agerbirk, Carl Erik Olsen, Luisa Ugolini, Susanna Cinti, Luca Lazzeri
A system of benzylic glucosinolates was found and characterized in common pepperweed, Lepidium densiflorum Schrad. The major glucosinolate was the novel 4-hydroxy-3,5-dimethoxybenzylglucosinolate (3,5-dimethoxysinalbin), present at high levels in seeds, leaves and roots. Medium level glucosinolates were 3,4-dimethoxybenzylglucosinolate and 3,4,5-trimethoxybenzylglucosinolate. Minor glucosinolates included benzylglucosinolate, 3-hydroxy and 3-methoxybenzylglucosinolate, 4-hydroxybenzylglucosinolate (sinalbin), the novel 4-hydroxy-3-methoxybenzylglucosinolate (3-methoxysinalbin) and indole-type glucosinolates...
March 25, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28342961/particulate-metal-bioaccessibility-in-physiological-fluids-and-cell-culture-media-toxicological-perspectives
#4
Bérénice Leclercq, Laurent Yves Alleman, Esperanza Perdrix, Véronique Riffault, Mélanie Happillon, Alain Strecker, Jean-Marc Lo-Guidice, Guillaume Garçon, Patrice Coddeville
According to the literature, tiny amounts of transition metals in airborne fine particles (PM2.5) may induce proinflammatory cell response through reactive oxygen species production. The solubility of particle-bound metals in physiological fluids, i.e. the metal bioaccessibility is driven by factors such as the solution chemical composition, the contact time with the particles, and the solid-to-liquid phase ratio (S/L). In this work, PM2.5-bound metal bioaccessibility was assessed in various physiological-like solutions including cell culture media in order to evidence the potential impact on normal human bronchial epithelial cells (NHBE) when studying the cytotoxicity and inflammatory responses of PM2...
March 23, 2017: Environmental Research
https://www.readbyqxmd.com/read/28342840/the-ratio-of-contrast-volume-to-glomerular-filtration-rate-predicts-acute-kidney-injury-and-mortality-after-transcatheter-aortic-valve-implantation
#5
Francesco Giannini, Azeem Latib, Richard J Jabbour, Massimo Slavich, Susanna Benincasa, Alaide Chieffo, Matteo Montorfano, Stefano Stella, Nicola Buzzatti, Ottavio Alfieri, Antonio Colombo
OBJECTIVE: The aim of this study was to assess the impact of the ratio of volume of contrast medium to the glomerular filtration rate (V/GFR) on acute kidney injury (AKI) after transcatheter aortic valve implantation (TAVI) and its impact on long-term mortality. METHODS: We retrospectively calculated V/GFR in 397 patients undergoing TAVI. AKI was defined as VARC-modified Risk, Injury, Failure, Loss and End-stage (RIFLE) kidney disease score≥2. RESULTS: The incidence of AKI was 17...
February 20, 2017: Cardiovascular Revascularization Medicine: Including Molecular Interventions
https://www.readbyqxmd.com/read/28341047/short-communication-effects-of-prill-size-of-a-palmitic-acid-enriched-fat-supplement-on-the-yield-of-milk-and-milk-components-and-nutrient-digestibility-of-dairy-cows
#6
J de Souza, J L Garver, C L Preseault, A L Lock
The objective of our experiment was to evaluate the effects of prill size of a palmitic acid-enriched fatty acid supplement (PA; 85% C16:0) on feed intake, nutrient digestibility, and production responses of dairy cows. Twenty-four primiparous and multiparous Holstein cows were assigned based on parity and production level to replicated 4×4 Latin squares balanced for carryover effects with 21-d periods. Treatments were a control diet (no added PA), or 2.0% PA added as a small prill size (PA-SM; 284±12.4µm), a medium prill size (PA-MD; 325±14...
January 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28341030/the-clinical-value-of-hepatic-extracellular-volume-fraction-using-routine-multiphasic-contrast-enhanced-liver-ct-for-staging-liver-fibrosis
#7
S L Guo, L N Su, Y N Zhai, W M Chirume, J Q Lei, H Zhang, L Yang, X P Shen, X X Wen, Y M Guo
AIM: To investigate the value of hepatic extracellular volume fractions (fECVs) measured using routine liver computed tomography (CT) evaluating liver fibrosis (LF). MATERIALS AND METHODS: A total of 60 patients (male:female ratio, 39:21; mean age, 42.4 years) histologically diagnosed with LF underwent routine liver CT. Absolute enhancement (in Hounsfield units) of the liver parenchyma (Eliver) and aorta (Eaorta) 3 minutes after contrast medium administration was calculated using precontrast and equilibrium phase scans...
March 2017: Clinical Radiology
https://www.readbyqxmd.com/read/28340620/stable-high-level-expression-of-factor-viii-in-chinese-hamster-ovary-cells-in-improved-elongation-factor-1-alpha-based-system
#8
Nadezhda A Orlova, Sergey V Kovnir, Alexandre G Gabibov, Ivan I Vorobiev
BACKGROUND: Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which the entire domain B is replaced by a short linker peptide, was approved for medical use. Efficacy and safety of this FVIII deletion variant are similar to full-length FVIII preparations while the level of production in CHO cells is substantially higher. Typical levels of productivity for CHO cell lines producing deletion variant FVIII-BDD SQ, described elsewhere, are 0...
March 24, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28337989/post-hoc-study-intravenous-hydration-treatment-in-chinese-patients-with-high-risk-of-contrast-induced-nephropathy-following-percutaneous-coronary-intervention
#9
Weijie Bei, Hualong Li, Kaiyang Lin, Kun Wang, Shiqun Chen, Xiaosheng Guo, Yong Liu, Ning Tan, Jiyan Chen
Contrast-induced nephropathy (CIN) develops after the injection of iodinated contrast media. This is a post hoc analysis of the data obtained from the TRUST study, which was a prospective, multicentre, observational study conducted to evaluate the safety and tolerability of the contrast medium iopromide in patients undergoing cardiac catheterization from August 2010 to September 2011 in China, conducted to explore the current status, trends and risk predictors of hydration treatment. The status of hydration to prevent CIN in each patient was recorded...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28335228/fabricating-water-dispersible-superparamagnetic-iron-oxide-nanoparticles-for-biomedical-applications-through-ligand-exchange-and-direct-conjugation
#10
Tina Lam, Pramod K Avti, Philippe Pouliot, Foued Maafi, Jean-Claude Tardif, Éric Rhéaume, Frédéric Lesage, Ashok Kakkar
Stable superparamagnetic iron oxide nanoparticles (SPIONs), which can be easily dispersed in an aqueous medium and exhibit high magnetic relaxivities, are ideal candidates for biomedical applications including contrast agents for magnetic resonance imaging. We describe a versatile methodology to render water dispersibility to SPIONs using tetraethylene glycol (TEG)-based phosphonate ligands, which are easily introduced onto SPIONs by either a ligand exchange process of surface-anchored oleic-acid (OA) molecules or via direct conjugation...
May 26, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28334245/coordinated-nitrogen-and-carbon-remobilization-for-nitrate-assimilation-in-leaf-sheath-and-root-and-associated-cytokinin-signals-during-early-regrowth-of-lolium-perenne
#11
Jessica Roche, Matthew H Turnbull, Qianqian Guo, Ondrej Novák, Jana Späth, Steven P Gieseg, Paula E Jameson, Jonathan Love
Background and Aims: The efficiency of N assimilation in response to defoliation is a critical component of plant regrowth and forage production. The aim of this research was to test the effect of the internal C/N balance on NO3- assimilation and to estimate the associated cytokinin signals following defoliation of perennial ryegrass ( Lolium perenne L. 'Grasslands Nui') plants. Methods: Plants, manipulated to have contrasting internal N content and contrasting availability of water soluble carbohydrates (WSCs), were obtained by exposure to either continuous light or short days (8:16 h light-dark), and watered with modified N-free Hoagland medium containing either high (5 m m ) or low (50 μ m ) NO3- as sole N source...
March 14, 2017: Annals of Botany
https://www.readbyqxmd.com/read/28332442/ultrasound-imaging-in-the-diagnosis-of-large-vessel-vasculitis
#12
Michael Czihal, Christian Lottspeich, Ulrich Hoffmann
Nowadays noninvasive vascular imaging has an important role in the diagnostic work-up of the large vessel vasculitides (LVV), most importantly giant cell arteritis (GCA) and Takayasu arteritis. Among the imaging modalities available, ultrasound (US) has several important advantages, including low costs, rapid and repetitive availability without exposure to radiation, and high spatial resolution for assessment of large and medium-sized arteries. Therefore, US can be regarded the first line imaging method in suspected LVV...
March 23, 2017: VASA. Zeitschrift Für Gefässkrankheiten
https://www.readbyqxmd.com/read/28330439/impact-of-preoperative-anemia-and-postoperative-hemoglobin-drop-on-the-incidence-of-acute-kidney-injury-and-in-hospital-mortality-in-patients-with-type-b-acute-aortic-syndromes-undergoing-thoracic-endovascular-aortic-repair
#13
Riccardo Gorla, Konstantinos Tsagakis, Michael Horacek, Amir-Abbas Mahabadi, Philipp Kahlert, Heinz Jakob, Eduardo Bossone, Raimund Erbel, Rolf Alexander Jánosi
BACKGROUND: The impact of preoperative anemia and postoperative hemoglobin (Hb) drop on the incidence of acute kidney injury (AKI) after thoracic endovascular aortic repair (TEVAR) for type B acute aortic syndromes (AAS) as well as their prognostic value is unknown. METHODS: This retrospective study included 144 patients with type B AAS undergoing TEVAR at our center. Preoperative anemia was classified as no/mild (Hb ≥ 12.0 g/dL in men; ≥11.3 g/dL in women), moderate (Hb 10...
April 2017: Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/28330433/impact-of-renal-arterial-morphology-on-fluoroscopy-time-in-chimney-endovascular-aneurysm-repair
#14
Masayuki Sugimoto, Giovanni Torsello, Konstantinos P Donas
Objectives To evaluate the impact of renal artery morphology on fluoroscopy time in chimney endovascular aneurysm repair. Methods Morphology of renal artery was retrospectively quantified in the preoperative CT angiography. Renal artery angulation, ostial diameter, severity of ostial stenosis, main renal artery length, and tortuosity were analyzed with a 3D workstation. The main measure outcome was fluoroscopic time as an indicator of complexity. Uni- and multivariate logistic regression analyses were performed to determine predictors of longer fluoroscopy...
January 1, 2017: Vascular
https://www.readbyqxmd.com/read/28326663/a-morphometric-study-of-the-canine-skull-and-periorbita-and-its-implications-for-regional-ocular-anesthesia
#15
Paulo Roberto Klaumann, Juan Carlos Duque Moreno, Fabiano Montiani-Ferreira
OBJECTIVE: A hypothetical relationship between canine cranial length and the length of the periorbita could be used for intraconal anesthetic volume estimation. STUDY SUBJECTS: Forty-one canine cadaver heads and one macerated dog skull. PROCEDURES: Inion and nasion points were recognized in the macerated skull and used as landmarks for cranial length measure. Thirty cadavers classified as dolichocephalic, mesaticephalic and brachycephalic were distributed in three study groups...
March 22, 2017: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/28323634/effect-of-laser-pulse-propagation-on-ultrafast-magnetization-dynamics-in-a-birefringent-medium
#16
J A de Jong, A M Kalashnikova, R V Pisarev, A M Balbashov, A V Kimel, A Kirilyuk, Th Rasing
Light propagation effects can strongly influence the excitation and the detection of laser-induced magnetization dynamics. We investigated experimentally and analytically the effects of crystallographic linear birefringence on the excitation and detection of ultrafast magnetization dynamics in the rare-earth orthoferrites (Sm0.5Pr0.5)FeO3 and (Sm0.55Tb0.45)FeO3, which possess weak and strong linear birefringence, respectively. Our finding is that the effect of linear birefringence on the result of a magneto-optical pump-probe experiment strongly depends on the mechanism of excitation...
April 26, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/28322045/9-73-efficiency-nonfullerene-all-organic-small-molecule-solar-cells-with-absorption-complementary-donor-and-acceptor
#17
Haijun Bin, Yankang Yang, Zhi-Guo Zhang, Long Ye, Masoud Ghasemi, Shanshan Chen, Yindong Zhang, Chunfeng Zhang, Chenkai Sun, Ling-Wei Xue, Changduk Yang, Harald Ade, Yongfang Li
In the last two years, polymer solar cells (PSCs) developed quickly with n-type organic semiconductor (n-OSs) as acceptor. In contrast, the research progress of nonfullerene organic solar cells (OSCs) with organic small molecule as donor and the n-OS as acceptor lags behind. Here, we synthesized a D-A structured medium bandgap organic small molecule H11 with bithienyl-benzodithiophene (BDT) as central donor unit and fluorobenzotriazole as acceptor unit, and achieved a power conversion efficiency (PCE) of 9...
March 21, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28320877/functional-analysis-of-the-alternative-sigma-28-factor-flia-and-its-anti-sigma-factor-flgm-of-the-non-flagellated-legionella-species-l-oakridgensis
#18
Hana Tlapák, Kerstin Rydzewski, Tino Schulz, Dennis Weschka, Eva Schunder, Klaus Heuner
Legionella oakridgensis is causing the Legionnaires' disease but is known to be less virulent than Legionella pneumophilaL. oakridgensis is one of the Legionella species that is non-flagellated. The genes of the flagellar regulon are absent; except the alternative sigma-28 factor (FliA) and its anti-sigma-28 factor (FlgM). Similar to L. oakridgensis, Legionella adelaidensis and Legionella londiniensis, located in the same phylogenetic clade, they have no flagellar regulon, although both are positive for fliA and flgM Herein, we investigated the role and function of both genes to better understand the role of FliA, the positive regulator of flagellin expression, in non-flagellated strains...
March 20, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28320610/carbon-monoxide-releasing-molecule-401-suppresses-polymorphonuclear-leukocyte-migratory-potential-by-modulating-f-actin-dynamics
#19
Ken Inoue, Eric K Patterson, Alfredo Capretta, Abdel R Lawendy, Douglas D Fraser, Gediminas Cepinskas
Carbon monoxide-releasing molecules (CORMs) suppress inflammation by reducing polymorphonuclear leukocyte (PMN) recruitment to the affected organs. We investigated modulation of PMN-endothelial cell adhesive interactions by water-soluble CORM-401 using an experimental model of endotoxemia in vitro. Human umbilical vein endothelial cells grown on laminar-flow perfusion channels were stimulated with 1 μg/mL lipopolysaccharide for 6 hours and perfused with 100 μmol/L CORM-401 (or inactive compound iCORM-401)-pretreated PMN for 5 minutes in the presence of 1...
March 17, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28319866/contrast-enhanced-ultrasound-and-computed-tomography-findings-of-granulomatosis-with-polyangiitis-presenting-with-multiple-intrarenal-microaneurysms-a-case-report
#20
Youe Ree Kim, Young Hwan Lee, Jong-Ho Lee, Kwon-Ha Yoon
Granulomatosis with polyangiitis (GPA) is a systemic disorder that affects small- and medium- sized vessels in many organs. Although the kidneys are the second most commonly involved organ in patients with GPA, its manifestation as multiple intrarenal aneurysms is rare. We report an unusual manifestation of GPA with multiple intrarenal microaneurysms, as demonstrated by contrast-enhanced ultrasound and computed tomography.
March 9, 2017: Clinical Imaging
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