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https://www.readbyqxmd.com/read/29783268/lipidomic-components-alterations-of-human-follicular-fluid-reveal-the-relevance-of-improving-clinical-outcomes-in-women-using-progestin-primed-ovarian-stimulation-compared-to-short-term-protocol
#1
Xiaowei Wen, Yanping Kuang, Lixia Zhou, Baofeng Yu, Qiuju Chen, Yonglun Fu, Zheng Yan, Haiyan Guo, Qifeng Lyu, Jun Xie, Weiran Chai
BACKGROUND Increasing the success rate of in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) is a duty of clinicians that has made many seek a variety of protocols. This study was undertaken to use a liquid chromatography-mass spectrometry (LC-MS) to define the alterations of follicular fluid (FF) lipid metabolites in patients undergoing progestin-primed ovarian stimulation (PPOS) compared with short-term protocol, revealing potential correlations between the differentially expressed lipids and ameliorative clinical outcomes...
May 21, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29778272/development-of-the-waveless-bovine-model
#2
F Jimenez-Krassel, J L H Ireland, C Kronemeyer, A Wilson-Alvarado, J J Ireland
Development of a bovine model without ovarian follicular waves (waveless) and transient increases in gonadotropin secretion during estrous cycles may lead to new methods to more consistently regulate ovulatory follicle growth thereby improving efficiency of embryo transfer. We hypothesized that the GnRH antagonist acyline would inhibit gonadotropin secretion thereby blocking follicular waves, ovarian function and ovulation during estrous cycles of cattle. To test this hypothesis, beef heifers (n = 5 per group) were treated twice daily with vehicle (control) or 25 or 50 μg/kg acyline beginning 12 h after GnRH-induced ovulation and ending 21 days later...
May 15, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29778102/effects-of-extending-the-length-of-pro-oestrus-in-an-oestradiol-and-progesterone-based-oestrus-synchronisation-program-on-ovarian-function-uterine-environment-and-pregnancy-establishment-in-beef-heifers
#3
J J de la Mata, R Núñez-Olivera, F Cuadro, D Bosolasco, V de Brun, A Meikle, G A Bó, A Menchaca
The aim of the present study was to investigate the effects of a strategy for extending pro-oestrus (the interval between luteolysis and ovulation) in an oestrus synchronisation protocol (named J-Synch) in beef heifers on follicular growth, sexual steroid concentrations, the oestrogen receptor ERα and progesterone receptors (PR) in the uterus, insulin-like growth factor (IGF) 1 and pregnancy rates. In Experiment 1, heifers treated with the new J-Synch protocol had a longer pro-oestrus period than those treated with the conventional protocol (mean (±s...
May 21, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29776717/m2-macrophages-promote-wound-induced-hair-neogenesis
#4
Akira Kasuya, Taisuke Ito, Yoshiki Tokura
BACKGROUND: De novo hair regeneration occurs in scars of normal adult mice. This interesting phenomenon is termed as wound-induced hair neogenesis (WIHN). We hypothesized that M2 macrophages are crucially involved in WIHN. OBJECTIVE: To clarify the contribution of M2 macrophages to WIHN. METHOD: We established a mouse model of WIHN. A full thickness skin excision was implemented on the back of C57BL/6 (B6) mice. Newly developing hair follicles were detected by a whole-mount assay...
May 12, 2018: Journal of Dermatological Science
https://www.readbyqxmd.com/read/29752954/regulation-of-the-activins-follistatins-inhibins-axis-by-energy-status-impact-on-reproductive-function
#5
Nikolaos Perakakis, Jagriti Upadhyay, Wael Ghaly, Joyce Chen, Pavlina Chrysafi, Athanasios D Anastasilakis, Christos S Mantzoros
BACKGROUND: We have previously demonstrated that the adipose tissue derived hormone leptin controls reproductive function by regulating the hypothalamic-pituitary-gonadal axis in response to energy deficiency. Here, we evaluate the activins-follistatins-inhibins (AFI) axis during acute (short-term fasting in healthy people) and chronic energy deficiency (women with hypothalamic amenorrhea due to strenuous exercise [HA]) and investigate their relation to leptin and reproductive function in healthy subjects and subjects with HA...
May 9, 2018: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/29716627/microrna-424-503-cluster-members-regulate-bovine-granulosa-cell-proliferation-and-cell-cycle-progression-by-targeting-smad7-gene-through-activin-signalling-pathway
#6
Hari Om Pande, Dawit Tesfaye, Michael Hoelker, Samuel Gebremedhn, Eva Held, Christiane Neuhoff, Ernst Tholen, Karl Schellander, Dessie Salilew Wondim
BACKGROUND: The granulosa cells are indispensable for follicular development and its function is orchestrated by several genes, which in turn posttranscriptionally regulated by microRNAs (miRNA). In our previous study, the miRRNA-424/503 cluster was found to be highly abundant in bovine granulosa cells (bGCs) of preovulatory dominant follicle compared to subordinate counterpart at day 19 of the bovine estrous cycle. Other study also indicated the involvement of miR-424/503 cluster in tumour cell resistance to apoptosis suggesting this miRNA cluster may involve in cell survival...
May 1, 2018: Journal of Ovarian Research
https://www.readbyqxmd.com/read/29684814/transcriptional-and-translational-abundance-of-bone-morphogenetic-protein-bmp-2-4-6-7-and-their-receptors-bmpr1a-1b-and-bmpr2-in-buffalo-ovarian-follicle-and-the-role-of-bmp4-and-bmp7-on-estrogen-production-and-survival-of-cultured-granulosa-cells
#7
G Rajesh, S R Mishra, A Paul, M Punetha, G M Vidyalakshmi, K Narayanan, S Bag, S K Bhure, V Singh Chouhan, V P Maurya, G Singh, Mihir Sarkar
BMPs and their receptors modulate the granulosa cell (GC) function in the follicle of domestic animals. Since little is known on BMPs in the buffalo, the present study was aimed to investigate the expression of BMP2, 4, 6, 7 and their receptors BMPR1A, BMPR1B, BMPR2 in the GC and theca cells (TC) of ovarian follicles and the role of BMP4 and BMP7 on buffalo GC. Follicles were classified into four groups based on size and E2 level in the follicular fluid as follows: (i) Group1(4-6 mm; <0.5 ng/mL) (ii) Group 2 (7-9 mm; 0...
April 16, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29684365/leucocyte-dynamics-during-the-evolution-of-human-coronary-atherosclerosis-conclusions-from-a-7-fold-chromogen-based-immunohistochemical-evaluation
#8
Melissa Zuiderwijk, Marlieke Geerts, Connie Janssen van Rhijn, Antoon van den Bogaerdt, Jaap F Hamming, Rogier A van Dijk, Jan H Lindeman
Atherosclerosis is a complex process with strong inflammatory component. We developed a straightforward 7-fold staining protocol for simultaneous assessment of dominant leucocyte classes, vascularization, and expression of the putative foam cell maker CD36. The method was applied on human coronaries covering the full spectrum of atherosclerotic disease. Results confirm the progressive association of macrophages and T-cells with the process, and a global presence of mast cells. B-cells exclusively present in adventitial follicles that accompany the process plaque destabilization (thin cap and ruptured lesions) and are otherwise absent...
April 20, 2018: American Journal of Pathology
https://www.readbyqxmd.com/read/29684161/impact-of-heat-stress-during-the-follicular-phase-on-porcine-ovarian-steroidogenic-and-phosphatidylinositol-3-signaling
#9
M J Dickson, C L Hager, A Al-Shaibi, P Q Thomas, L H Baumgard, J W Ross, A F Keating
Environmental conditions that impede heat dissipation and increase body temperature cause heat stress (HS). The study objective was to evaluate impacts of HS on the follicular phase of the estrous cycle. Post-pubertal gilts (126.0 ± 21.6 kg) were orally administered altrenogest to synchronize estrus, and subjected to either 5 d of thermal neutral (TN; 20.3 ± 0.5°C; n = 6) or cyclical HS (25.4 - 31.9°C; n = 6) conditions during the follicular phase preceding behavioral estrus. On d 5, blood samples were obtained, gilts were euthanized, and ovaries collected...
April 19, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29643837/insulin-like-growth-factor-binding-proteins-and-igfbp-proteases-a-dynamic-system-regulating-the-ovarian-folliculogenesis
#10
REVIEW
Sabine Mazerbourg, Philippe Monget
The aim of the present article is to update our understanding of the expression of the insulin-like growth factor binding proteins (IGFBPs), IGFBP proteases and their implication in the different processes of ovarian folliculogenesis in mammals. In the studied species, IGFs and several small-molecular weight IGFBPs (in particular IGFBP-2 and IGFBP-4) are considered, respectively, as stimulators and inhibitors of follicular growth and maturation. IGFs play a key role in sensitizing ovarian granulosa cells to FSH action during terminal follicular growth...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29610059/gonadotropin-concentrations-associated-with-variations-in-diameter-deviation-during-follicle-selection-in-holstein-heifers
#11
O J Ginther, R R Domingues, S V Dangudubiyyam, E R Araujo
Diameter deviation or selection of the future dominant follicle (F1) from the future largest subordinate follicle (F2) during a follicular wave occurs when F1 is 8.5 mm (expected deviation, day 0). Deviation has been classified as conventional (F2 ≥ 7.0 mm), F2-undersized (F2 < 7.0 mm), and F1,F2-switched (F2 larger than F1 on day -1 or 0). Concentrations of gonadotropins were compared within and among deviation classifications in waves 1 and 2 in 48 heifers. A three-way (wave 1 compared with 2, classification, day) analysis indicated no effect of wave 1 compared with 2 on F2 or FSH...
March 26, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29609180/angiocoupling-between-the-dominant-follicle-and-corpus-luteum-during-waves-1-and-2-in-bos-taurus-heifers
#12
R R Domingues, O J Ginther
The local two-way effect of the future and established dominant follicle (DF) and corpus luteum (CL) on color-Doppler signals of blood flow on each other (angiocoupling) was studied from ovulation to 16 days after ovulation during wave 1 and for the first time in wave 2 in Bos taurus heifers (N = 24). Identity of follicles greater than 4 mm was maintained from day to day. Blood-flow signals in the follicle wall and in the CL were estimated after scanning the entire structure. Ovarian patterns were defined based on the presence of a DF and CL: DF-CL pattern (DF and CL in the same ovary), DF pattern or CL pattern (DF and CL in opposite ovaries), and devoid pattern (neither DF nor CL)...
March 21, 2018: Theriogenology
https://www.readbyqxmd.com/read/29584617/constitutively-active-follicle-stimulating-hormone-receptor-enables-androgen-independent-spermatogenesis
#13
Olayiwola O Oduwole, Hellevi Peltoketo, Ariel Poliandri, Laura Vengadabady, Marcin Chrusciel, Milena Doroszko, Luna Samanta, Laura Owen, Brian Keevil, Nafis A Rahman, Ilpo T Huhtaniemi
Spermatogenesis is regulated by the 2 pituitary gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH). This process is considered impossible without the absolute requirement of LH-stimulated testicular testosterone (T) production. The role of FSH remains unclear because men and mice with inactivating FSH receptor (FSHR) mutations are fertile. We revisited the role of FSH in spermatogenesis using transgenic mice expressing a constitutively strongly active FSHR mutant in a LH receptor-null (LHR-null) background...
March 26, 2018: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29582411/the-influence-of-endometrial-thickness-on-pregnancy-rates-in-modified-natural-cycle-frozen-thawed-embryo-transfer
#14
Eva R Groenewoud, Ben J Cohlen, Amani Al-Oraiby, Egbert A Brinkhuis, Frank J M Broekmans, Jan-Peter de Bruin, Grada van Dool, Katrin Fleisher, Jaap Friederich, Mariëtte Goddijn, Annemieke Hoek, Diederik A Hoozemans, Eugenie M Kaaijk, Caroliene A M Koks, Joop S E Laven, Paul J Q van der Linden, Petra A Manger, Minouche van Rumste, Taeke Spinder, Nick S Macklon
INTRODUCTION: Pregnancy after frozen-thawed embryo transfer (FET) is a multifactorial process. While embryo quality is a key factor in determining pregnancy, other factors, including maternal determinants are also considered to be predictive. Even though an association between endometrial thickness measured by transvaginal ultrasound and pregnancy rates has been reported in patients undergoing various assisted reproductive technology treatments, whether endometrial thickness predicts achieving pregnancy after natural cycle FET (NC-FET) remains unclear...
March 27, 2018: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/29577917/msx2-supports-epidermal-competency-during-wound-induced-hair-follicle-neogenesis
#15
Michael W Hughes, Ting-Xin Jiang, Maksim V Plikus, Christian Fernando Guerrero-Juarez, Chein-Hong Lin, Christopher Schafer, Robert Maxson, Randall B Widelitz, Cheng-Ming Chuong
Cutaneous wounds in adult mammals typically heal by scarring. However, large full-thickness wounds undergo wound-induced hair follicle neogenesis (WIHN), a novel form of regeneration. Here, we demonstrate WIHN requires transient expression of epidermal Msx2 in two phases; the wound margin early and the wound center late. Msx2 expression is present in the migrating epithelium during early wound healing, and then presents in the epithelium and mesenchyme later in the wound center. WIHN is abrogated in germline and epithelial-specific Msx2 mutant mice...
March 22, 2018: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/29573406/th17-treg-imbalance-in-hidradentitis-suppurativa-acne-inversa-the-link-to-hair-follicle-dissection-obesity-smoking-and-autoimmune-comorbidities
#16
REVIEW
B C Melnik, S M John, W Chen, G Plewig
BACKGROUND: Disintegration of the infundibula of terminal hair follicles (HF) in intertriginous skin areas exhibits the histological hallmark of hidradenitis suppurativa (HS)/acne inversa, featuring a dissecting terminal hair folliculitis. Elevated serum levels of interleukin 17 and local increase in the ratio of proinflammatory Th17 cells and antiinflammatory regulatory T cells (Tregs) have been reported. Perifollicular Tregs play a key role for HF stem cell homeostasis and infundibular integrity...
March 24, 2018: British Journal of Dermatology
https://www.readbyqxmd.com/read/29566475/-identification-of-nodular-gastritis-among-patients-diagnosed-with-lymphofollicular-gastritis-on-a-gastric-biopsied-specimen
#17
Young Jung Kim, Sun Young Lee, Sang Pyo Lee, Jeong Hwan Kim, In Kyung Sung, Hyung Seok Park, Chan Sup Shim, Hye Seung Han
Background/Aims: Nodular gastritis (NG) is a well-known endoscopic finding observed in patients with a Helicobacter pylori infection, which may lead to invasive gastric cancer. Lymphofollicular gastritis consists of lymphoid follicles or lymphoid cell aggregates, and is common in children. The aim of this study was to identify patients with NG from those in whom gastric biopsied specimens showed lymphoid follicles and lymphoid cell aggregates. Methods: Subjects, whose gastric biopsy specimens showed lymphoid follicles or lymphoid cell aggregates, were included in this study...
March 25, 2018: Korean Journal of Gastroenterology, Taehan Sohwagi Hakhoe Chi
https://www.readbyqxmd.com/read/29550272/synchronization-of-time-of-ovarian-follicular-development-in-llamas-lama-glama-using-a-protocol-based-on-gnrh-and-pgf-2%C3%AE
#18
C P Bianchi, M A Benavente, M Simonetti, M A Aba
The aim of this study was to evaluate the effectiveness of a protocol based on GnRH and PGF2α to synchronize the emergence of a new wave of ovarian follicular development in llamas and, therefore, when a new dominant follicle develops. Llamas (n = 18) were assigned to growing, mature or regressing follicle groups according to the phase of the follicular wave at the beginning of treatment. The protocol was initiated with a GnRH analogue (GnRHa) injection on Day 0 followed 7 days later with a d-cloprostenol injection and a second GnRHa injection on Day 10...
May 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29544632/involvement-of-bone-morphogenetic-proteins-bmp-in-the-regulation-of-ovarian-function
#19
Sheena L P Regan, Phil G Knight, John L Yovich, Yee Leung, Frank Arfuso, Arun Dharmarajan
Primordial germ cells migrate to the fetal gonads and proliferate during gestation to generate a fixed complement of primordial follicles, the so-called ovarian reserve. Primordial follicles comprise an oocyte arrested at the diplotene stage of meiosis, surrounded by a layer of pregranulosa cells. Activation of primordial follicles to grow beyond this arrested stage is of particular interest because, once activated, they are subjected to regulatory mechanisms involved in growth, selection, maturation, and ultimately, ovulation or atresia...
2018: Vitamins and Hormones
https://www.readbyqxmd.com/read/29544123/concentrations-of-follicle-stimulating-hormone-associated-with-follicle-selection-number-of-follicles-and-ipsilateral-vs-contralateral-relationships-in-mares
#20
O J Ginther, R R Domingues, S V Dangudubiyyam, E R Araujo
Diameter deviation or follicle selection during a follicular wave begins with continuous growth rate of F1 (dominant or selected follicle; DF) and decreasing growth rate of F2 (largest subordinate). Intraovarian patterns based on presence or absence of the DF and CL are DF-CL, DF, CL, and devoid (neither DF nor CL). The DF and CL relationships in a pair of ovaries are ipsilateral (DF-CL pattern and devoid pattern) and contralateral (DF pattern and CL pattern). The effects of patterns and relationships on F1, F2, and FSH during deviation were determined in 21 mares...
June 2018: Theriogenology
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