keyword
MENU ▼
Read by QxMD icon Read
search

Cortactin

keyword
https://www.readbyqxmd.com/read/28341841/regulation-of-membrane-ruffling-by-polarized-stim1-and-orai1-in-cortactin-rich-domains
#1
Aida M Lopez-Guerrero, Patricia Tomas-Martin, Carlos Pascual-Caro, Thomas Macartney, Alejandro Rojas-Fernandez, Graeme Ball, Dario R Alessi, Eulalia Pozo-Guisado, Francisco Javier Martin-Romero
Cell motility and migration requires the reorganization of the cortical cytoskeleton at the leading edge of cells and extracellular Ca(2+) entry is essential for this reorganization. However the molecular nature of the regulators of this pathway is unknown. This work contributes to understanding the role of STIM1 and ORAI1 in the promotion of membrane ruffling by showing that phospho-STIM1 localizes at the leading edge of cells, and that both phospho-STIM1 and ORAI1 co-localize with cortactin (CTTN), a regulator of the cytoskeleton at membrane ruffling areas...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28335271/distribution-of-iron-oxide-core-titanium-dioxide-shell-nanoparticles-in-vx2-tumor-bearing-rabbits-introduced-by-two-different-delivery-modalities
#2
Tamer Refaat, Derek West, Samar El Achy, Vamsi Parimi, Jasmine May, Lun Xin, Kathleen R Harris, William Liu, Michael Beau Wanzer, Lydia Finney, Evan Maxey, Stefan Vogt, Reed A Omary, Daniele Procissi, Andrew C Larson, Tatjana Paunesku, Gayle E Woloschak
This work compares intravenous (IV) versus fluoroscopy-guided transarterial intra-catheter (IC) delivery of iron oxide core-titanium dioxide shell nanoparticles (NPs) in vivo in VX2 model of liver cancer in rabbits. NPs coated with glucose and decorated with a peptide sequence from cortactin were administered to animals with developed VX2 liver cancer. Two hours after NPs delivery tumors, normal liver, kidney, lung and spleen tissues were harvested and used for a series on histological and elemental analysis tests...
August 3, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28306714/reconstruction-and-signal-propagation-analysis-of-the-syk-signaling-network-in-breast-cancer-cells
#3
Aurélien Naldi, Romain M Larive, Urszula Czerwinska, Serge Urbach, Philippe Montcourrier, Christian Roy, Jérôme Solassol, Gilles Freiss, Peter J Coopman, Ovidiu Radulescu
The ability to build in-depth cell signaling networks from vast experimental data is a key objective of computational biology. The spleen tyrosine kinase (Syk) protein, a well-characterized key player in immune cell signaling, was surprisingly first shown by our group to exhibit an onco-suppressive function in mammary epithelial cells and corroborated by many other studies, but the molecular mechanisms of this function remain largely unsolved. Based on existing proteomic data, we report here the generation of an interaction-based network of signaling pathways controlled by Syk in breast cancer cells...
March 17, 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28293874/diversity-of-actin-architecture-in-human-osteoclasts-network-of-curved-and-branched-actin-supporting-cell-shape-and-intercellular-micrometer-level-tubes
#4
Paula Pennanen, Maria Helena Alanne, Elnaz Fazeli, Takahiro Deguchi, Tuomas Näreoja, Sirkku Peltonen, Juha Peltonen
Osteoclasts are multinucleated bone-resorbing cells with a dynamic actin cytoskeleton. Osteoclasts are derived from circulating mononuclear precursors. Confocal and stimulated emission depletion (STED) super-resolution microscopy was used to investigate peripheral blood-derived human osteoclasts cultured on glass surfaces. STED and confocal microscopy demonstrated that the actin was curved and branched, for instance, in the vicinity of membrane ruffles. The overall architecture of the curved actin network extended from the podosomes to the top of the cell...
March 14, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28287395/p27-kip1-promotes-invadopodia-turnover-and-invasion-through-the-regulation-of-the-pak1-cortactin-pathway
#5
Pauline Jeannot, Ada Nowosad, Renaud T Perchey, Caroline Callot, Evangeline Bennana, Takanori Katsube, Patrick Mayeux, François Guillonneau, Stéphane Manenti, Arnaud Besson
p27(Kip1) (p27) is a cyclin-CDK inhibitor and negative regulator of cell proliferation. p27 also controls other cellular processes including migration and cytoplasmic p27 can act as an oncogene. Furthermore, cytoplasmic p27 promotes invasion and metastasis, in part by promoting epithelial to mesenchymal transition. Herein, we find that p27 promotes cell invasion by binding to and regulating the activity of Cortactin, a critical regulator of invadopodia formation. p27 localizes to invadopodia and limits their number and activity...
March 13, 2017: ELife
https://www.readbyqxmd.com/read/28235780/inhibitory-cortactin-nanobodies-delineate-the-role-of-nta-and-sh3-domain-specific-functions-during-invadopodium-formation-and-cancer-cell-invasion
#6
Laurence Bertier, Ciska Boucherie, Olivier Zwaenepoel, Berlinda Vanloo, Marleen Van Troys, Isabel Van Audenhove, Jan Gettemans
Cancer cells exploit different strategies to escape from the primary tumor, gain access to the circulation, disseminate throughout the body, and form metastases, the leading cause of death by cancer. Invadopodia, proteolytically active plasma membrane extensions, are essential in this escape mechanism. Cortactin is involved in every phase of invadopodia formation, and its overexpression is associated with increased invadopodia formation, extracellular matrix degradation, and cancer cell invasion. To analyze endogenous cortactin domain function in these processes, we characterized the effects of nanobodies that are specific for the N-terminal acidic domain of cortactin and expected to target small epitopes within this domain...
February 24, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28166391/lactosamine-based-derivatives-as-tools-to-delineate-the-biological-functions-of-galectins-application-to-skin-tissue-repair
#7
Cyrille Grandjean, Johann Dion, Frédérique Deshayes, Nataliya Storozhylova, Tamara Advedissian, Annie Lambert, Mireille Viguier, Charles Tellier, Christophe Dussouy, Françoise Poirier
Galectins have been recognized as potential novel therapeutic targets in connection with the numerous fundamental biological processes which they are involved in. Galectins are key players of homeostasis and as such, their expression and function are finely tuned in vivo. As a consequence, their modes of action are complex and remain largely unexplored partly due the lack of dedicated tools. We thus thought to design galectin inhibitors from lactosamine core, functionalized at key C2 and C3' positions by aromatic subsituents to ensure both high affinity and selectivity, and equipped with a spacer which can be modified on demand to further modulate their intrinsic physico-chemical properties...
February 6, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28131549/cortactin-and-phosphorylated-cortactin-tyr-466-expression-in-temporal-bone-carcinoma
#8
Gino Marioni, Elisabetta Zanoletti, Antonio Mazzoni, Andrea Gianatti, Elisa Valentini, Laura Girasoli, Martina Guariento, Luciano Giacomelli, Alessandro Martini, Stella Blandamura
PURPOSE: Cortactin is a multidomain protein engaged in several cellular mechanisms involving actin assembly and cytoskeletal arrangement. Cortactin overexpression in several malignancies has been associated with increased cell migration, invasion, and metastatic potential. Cortactin needs to be activated by tyrosine or serine/threonine phosphorylation. The role of cortactin and phosphorylated cortactin (residue tyr(466)) was investigated in temporal bone squamous cell carcinoma (TBSCC)...
March 2017: American Journal of Otolaryngology
https://www.readbyqxmd.com/read/28126411/perfluorooctanoic-acid-affects-endocytosis-involving-clathrin-light-chain-a-and-microrna-133b-3p-in-mouse-testes
#9
Yin Lu, Jianshe Wang, Xuejiang Guo, Shengmin Yan, Jiayin Dai
Perfluorooctanoic acid (PFOA) is an abundant perfluoroalkyl substance widely applied in industrial and consumer products. Among its potential health hazards, testicular toxicity is of major concern. To explore the potential effect of miRNA on post-translational regulation after PFOA exposure, changes in miRNAs were detected via miRNA array. Seventeen miRNAs were differentially expressed (eight upregulated, nine downregulated) in male mouse testes after exposure to 5mg/kg/d of PFOA for 28d (>1.5-fold and P<0...
March 1, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28123080/cortactin-is-a-regulator-of-activity-dependent-synaptic-plasticity-controlled-by-wingless
#10
Daniel Alicea, Marizabeth Perez, Carolina Maldonado, Carihann Dominicci-Cotto, Bruno Marie
Major signaling molecules initially characterized as key early developmental regulators are also essential for the plasticity of the nervous system. Previously, the Wingless (Wg)/Wnt pathway was shown to underlie the structural and electrophysiological changes during activity-dependent synaptic plasticity at the Drosophila neuromuscular junction. A challenge remains to understand how this signal mediates the cellular changes underlying this plasticity. Here, we focus on the actin regulator Cortactin, a major organizer of protrusion, membrane mobility, and invasiveness, and define its new role in synaptic plasticity...
February 22, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28122813/hs1-deficiency-impairs-neutrophil-recruitment-in-vivo-and-activation-of-the-small-gtpases-rac1-and-rap1
#11
Joanna Latasiewicz, Annette Artz, Ding Jing, Mariana Pacheco Blanco, Silke M Currie, Martha Velázquez Avila, Michael Schnoor, Dietmar Vestweber
Neutrophil extravasation is a critical step of the innate immune system's response to inflammation. This multistep process is tightly regulated by adhesion and signaling molecules in the endothelium and neutrophils. Activation of the β2 integrin LFA-1 is critical for adhesion of leukocytes to postcapillary venules. This step requires coordinated activation of signaling pathways in chemokine-stimulated neutrophils, including GTPase activation and cytoskeletal remodeling, leading to conformational changes in LFA-1...
January 25, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28120846/cortactin-deficiency-causes-increased-rhoa-rock1-dependent-actomyosin-contractility-intestinal-epithelial-barrier-dysfunction-and-disproportionately-severe-dss-induced-colitis
#12
A F Citalán-Madrid, H Vargas-Robles, A García-Ponce, M Shibayama, A Betanzos, P Nava, C Salinas-Lara, K Rottner, R Mennigen, M Schnoor
The intestinal epithelium constitutes a first line of defense of the innate immune system. Epithelial dysfunction is a hallmark of intestinal disorders such as inflammatory bowel diseases (IBDs). The actin cytoskeleton controls epithelial barrier integrity but the function of actin regulators such as cortactin is poorly understood. Given that cortactin controls endothelial permeability, we hypothesized that cortactin is also important for epithelial barrier regulation. We found increased permeability in the colon of cortactin-KO mice that was accompanied by reduced levels of ZO-1, claudin-1, and E-cadherin...
January 25, 2017: Mucosal Immunology
https://www.readbyqxmd.com/read/28115390/inhibition-of-klf5-myo9b-rhoa-pathway-mediated-podosome-formation-in-macrophages-ameliorates-abdominal-aortic-aneurysm
#13
Dong Ma, Bin Zheng, Toru Suzuki, Ruonan Zhang, Chunyang Jiang, Disi Bai, Weina Yin, Zhan Yang, Xinhua Zhang, Lianguo Hou, Hong Zhan, Jin-Kun Wen
RATIONALE: Abdominal aortic aneurysms (AAAs) are characterized by pathological remodeling of the aortic wall. Although both increased Krüppel-like factor 5 (KLF5) expression and macrophage infiltration have been implicated in vascular remodeling, the role of KLF5 in macrophage infiltration and AAA formation remains unclear. OBJECTIVE: To determine the role of KLF5 in AAA formation and macrophage infiltration into AAAs. METHODS AND RESULTS: KLF5 expression was significantly increased in human AAA tissues and in two mouse models of experimental AAA...
January 23, 2017: Circulation Research
https://www.readbyqxmd.com/read/28068324/role-of-akt2-in-regulation-of-metastasis-suppressor-1-expression-and-colorectal-cancer-metastasis
#14
E Agarwal, C M Robb, L M Smith, M G Brattain, J Wang, J D Black, S Chowdhury
Survival signaling is critical for the metastatic program of cancer cells. The current study investigated the role of Akt survival proteins in colorectal cancer (CRC) metastasis and explored potential mechanisms of Akt-mediated metastasis regulation. Using an orthotopic implantation model in mice, which uniquely recapitulates the entire multistep process of CRC metastasis, combined with an inducible system of short hairpin RNA-mediated Akt isoform knockdown in human CRC cells, our studies confirm a role of Akt2 in CRC cell dissemination to distant organs in vivo...
January 9, 2017: Oncogene
https://www.readbyqxmd.com/read/28052035/brca1-iris-overexpression-promotes-and-maintains-the-tumor-initiating-phenotype-implications-for-triple-negative-breast-cancer-early-lesions
#15
Abhilasha Sinha, Bibbin T Paul, Lisa M Sullivan, Hillary Sims, Ahmed El Bastawisy, Hend F Yousef, Abdel-Rahman N Zekri, Abeer A Bahnassy, Wael M ElShamy
Tumor-initiating cells (TICs) are cancer cells endowed with self-renewal, multi-lineage differentiation, increased chemo-resistance, and in breast cancers the CD44+/CD24-/ALDH1+ phenotype. Triple negative breast cancers show lack of BRCA1 expression in addition to enhanced basal, epithelial-to-mesenchymal transition (EMT), and TIC phenotypes. BRCA1-IRIS (hereafter IRIS) is an oncogene produced by the alternative usage of the BRCA1 locus. IRIS is involved in induction of replication, transcription of selected oncogenes, and promoting breast cancer cells aggressiveness...
February 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28006832/the-dual-edema-preventing-molecular-mechanism-of-the-crataegus-extract-ws-1442-can-be-assigned-to-distinct-phytochemical-fractions
#16
Simone Fuchs, Iris Bischoff, Elisabeth A Willer, Jacqueline Bräutigam, Martin F Bubik, Clemens A J Erdelmeier, Egon Koch, Maria T Faleschini, Maria De Mieri, Milena Bauhart, Stefan Zahler, Andreas Hensel, Matthias Hamburger, Olivier Potterat, Robert Fürst
The hawthorn (Crataegus spp.) extract WS 1442 is used against mild forms of chronic heart failure. This disease is associated with endothelial barrier dysfunction and edema formation. We have recently shown that WS 1442 protects against this dysfunction by a dual mechanism: it both promotes endothelial barrier integrity by activation of a barrier-enhancing pathway (cortactin activation) and inhibits endothelial hyperpermeability by blocking a barrier disruptive pathway (calcium signaling). In this study, we aimed to identify the bioactive compounds responsible for these actions by using a bioactivity-guided fractionation approach...
December 22, 2016: Planta Medica
https://www.readbyqxmd.com/read/27974247/annexin-a2-is-critical-for-blood-testis-barrier-integrity-and-spermatid-disengagement-in-the-mammalian-testis
#17
Katarzyna Chojnacka, Barbara Bilinska, Dolores D Mruk
Throughout spermatogenesis, two important processes occur at late stage VIII of the seminiferous epithelial cycle in the rat testis: preleptotene spermatocytes commence entry into the adluminal compartment and step 19 spermatids release from the seminiferous epithelium. Presently, it is not clear how these processes, which involve extensive restructuring of unique Sertoli-Sertoli and Sertoli-germ cell junctions, are mediated. We aimed to determine whether annexin A2 (ANXA2), a Ca(2+)-dependent and phospholipid-binding protein, participates in cell junction dynamics...
December 11, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27959772/cellular-defense-or-viral-assist-the-dilemma-of-hdac6
#18
Kai Zheng, Yingchun Jiang, Zhendan He, Kaio Kitazato, Yifei Wang
AbstractHistone deacetylase 6 (HDAC6) is an unique cytoplasmic deacetylase that regulates various important biological processes via preventing protein aggregation and deacetylating different non-histone substrates including tubulin, heat shock protein 90 (Hsp90), cortactin, retinoic acid inducible gene I (RIG-1) and β-catenin. Growing evidences have indicated a dual role for HDAC6 in viral infection and pathogenesis: HDAC6 may represent a host defense mechanism against viral infection through modulating microtubule, triggering antiviral immune response and stimulating protective autophagy, or it may be hijacked by the virus to enhance proinflammatory response...
December 12, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27911942/persistent-morbillivirus-infection-leads-to-altered-cortactin-distribution-in-histiocytic-sarcoma-cells-with-decreased-cellular-migration-capacity
#19
Vanessa Maria Pfankuche, Mohamed Sayed-Ahmed, Vanessa Bono Contioso, Ingo Spitzbarth, Karl Rohn, Reiner Ulrich, Ulrich Deschl, Arno Kalkuhl, Wolfgang Baumgärtner, Christina Puff
Histiocytic sarcomas represent rare but fatal neoplasms in humans. Based on the absence of a commercially available human histiocytic sarcoma cell line the frequently affected dog displays a suitable translational model. Canine distemper virus, closely related to measles virus, is a highly promising candidate for oncolytic virotherapy. Therapeutic failures in patients are mostly associated with tumour invasion and metastasis often induced by misdirected cytoskeletal protein activities. Thus, the impact of persistent canine distemper virus infection on the cytoskeletal protein cortactin, which is frequently overexpressed in human cancers with poor prognosis, was investigated in vitro in a canine histiocytic sarcoma cell line (DH82)...
2016: PloS One
https://www.readbyqxmd.com/read/27903975/cortactin-promotes-colorectal-cancer-cell-proliferation-by-activating-the-egfr-mapk-pathway
#20
Xiaojian Zhang, Kun Liu, Tao Zhang, Zhenlei Wang, Xuan Qin, Xiaoqian Jing, Haoxuan Wu, Xiaopin Ji, Yonggang He, Ren Zhao
Cortactin (CTTN) is overexpressed in various tumors, including head and neck squamous cell carcinoma and colorectal cancer (CRC), and can serve as a biomarker of cancer metastasis. We observed that CTTN promotes cancer cell proliferation in vitro and increases CRC tumor xenograft growth in vivo. CTTN expression increases EGFR protein levels and enhances the activation of the MAPK signaling pathway. CTTN expression also inhibits the ubiquitin-mediated degradation of EGFR by suppressing the coupling of c-Cbl with EGFR...
January 3, 2017: Oncotarget
keyword
keyword
86035
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"