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JOURNAL ARTICLE
Ana Rita Rodrigues, André Oliveira, Tatiana Vieira, Rui Assis, Catarina Lume, João Gonçalves-Pereira, Susana M Fernandes
BACKGROUND: Patients with critical illness often survive the intensive care unit (ICU) at a cost of prolonged length of stay (LOS) and slow recovery. This chronic critically ill disease may lead to long-term poor outcomes, especially in older or frail patients. OBJECTIVES: The main goal of this study was to address the characteristics and outcomes of patients with prolonged ICU LOS. Mainly, short- and long-term admissions were compared to identify risk factors for persistent critical illness and to characterise the impact on ICU, hospital, and long-term mortality...
April 21, 2024: Australian Critical Care: Official Journal of the Confederation of Australian Critical Care Nurses
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JOURNAL ARTICLE
Sandra Fernandes, Carolina Costa, Ingrid Sayumi Nakamura, Rui Poínhos, Bruno M P M Oliveira
The transition to college is a period of higher risk of the development of eating disorders, with nutrition/dietetics students representing a group of particular vulnerability. Hence, it is interesting to assess eating disorders, taking into consideration potential sources of bias, including social desirability. Our aims were to compare the risk of eating disorders between students of nutrition/dietetics and those attending other courses and to study potential social desirability biases. A total of 799 higher education students (81...
March 29, 2024: Healthcare (Basel, Switzerland)
#3
JOURNAL ARTICLE
Carolina Freitas Henriques, Rui Fernandes, Francisco Barreto, Rubina Miranda, Teresa Carolina Aguiar
BACKGROUND: Hyperargininemia is a rare inherited metabolic disorder of the urea cycle with an autosomal recessive transmission. It occurs due to a deficiency of the enzyme arginase I and causes progressive neurological damage. Very few cases are diagnosed in adulthood, with the majority being diagnosed before the age of 4. Currently, this condition is diagnosed by a mass spectrometry technique in neonatal screening, which has been implemented in Portugal since 2007; births before that were not screened for this entity...
2024: European Journal of Case Reports in Internal Medicine
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JOURNAL ARTICLE
Jorge Gomes Lopes Barros, Raquel Fernandes, Ana Abraão, Rui Dias Costa, Alfredo Aires, Irene Gouvinhas, Daniel Granato, Ana Novo Barros
The historical use of plants as sources of natural compounds has persisted over time. Increasing the intake of bioactive substances shows significant potential for promoting overall well-being and health. This study delves into the pigments, phenolic composition, and profile, along with antioxidant properties, of leaf extracts rich in bioactives from plants in the Azores region, contributing to sustainable primary food production. Analyses encompassed chlorophylls, carotenoids, total phenols, ortho -diphenols, and flavonoids, as well as antioxidant capacity assessment, polyphenolic profiling, and quantification...
March 6, 2024: Antioxidants (Basel, Switzerland)
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JOURNAL ARTICLE
Ricardo Abrantes, Victor Pimentel, Mafalda N S Miranda, Ana Rita Silva, António Diniz, Bianca Ascenção, Carmela Piñeiro, Carmo Koch, Catarina Rodrigues, Cátia Caldas, Célia Morais, Domitília Faria, Elisabete Gomes da Silva, Eugénio Teófilo, Fátima Monteiro, Fausto Roxo, Fernando Maltez, Fernando Rodrigues, Guilhermina Gaião, Helena Ramos, Inês Costa, Isabel Germano, Joana Simões, Joaquim Oliveira, José Ferreira, José Poças, José Saraiva da Cunha, Jorge Soares, Sandra Fernandes, Kamal Mansinho, Liliana Pedro, Maria João Aleixo, Maria João Gonçalves, Maria José Manata, Margarida Mouro, Margarida Serrado, Micaela Caixeiro, Nuno Marques, Olga Costa, Patrícia Pacheco, Paula Proença, Paulo Rodrigues, Raquel Pinho, Raquel Tavares, Ricardo Correia de Abreu, Rita Côrte-Real, Rosário Serrão, Rui Sarmento E Castro, Sofia Nunes, Telo Faria, Teresa Baptista, Daniel Simões, Luis Mendão, M Rosário O Martins, Perpétua Gomes, Marta Pingarilho, Ana B Abecasis
INTRODUCTION: HIV late presentation (LP) remains excessive in Europe. We aimed to analyze the factors associated with late presentation in the MSM population newly diagnosed with HIV in Portugal between 2014 and 2019. METHODS: We included 391 newly HIV-1 diagnosed Men who have Sex with Men (MSM), from the BESTHOPE project, in 17 countrywide Portuguese hospitals. The data included clinical and socio-behavioral questionnaires and the viral genomic sequence obtained in the drug resistance test before starting antiretrovirals (ARVs)...
2024: Frontiers in Public Health
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JOURNAL ARTICLE
Cláudia Raquel Marques, Jonas Campos, Belém Sampaio-Marques, Filipa Ferreira Antunes, Raquel Medina Dos Santos Cunha, Deolinda Silva, Sandra Barata-Antunes, Rui Lima, Ana Fernandes-Platzgummer, Cláudia L da Silva, Rui Amandi Sousa, António José Salgado
BACKGROUND AIMS: Parkinson's disease (PD) is the second most common neurodegenerative disorder. The etiology of the disease remains largely unknown, but evidence have suggested that the overexpression and aggregation of alpha-synuclein (α-syn) play key roles in the pathogenesis and progression of PD. Mesenchymal stromal cells (MSCs) have been earning attention in this field, mainly due to their paracrine capacity. The bioactive molecules secreted by MSCs, i.e. their secretome, have been associated with enhanced neuronal survival as well as a strong modulatory capacity of the microenvironments where the disease develops...
February 18, 2024: Cytotherapy
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REVIEW
Rui Silva, Bruna Silva, Cristiana Fernandes, Pedro Morouço, Nuno Alves, António Veloso
When a limb suffers a fracture, rupture, or dislocation, it is traditionally immobilized with plaster. This may induce discomfort in the patient, as well as excessive itching and sweating, which creates the growth of bacteria, leading to an unhygienic environment and difficulty in keeping the injury clean during treatment. Furthermore, if the plaster remains for a long period, it may cause lesions in the joints and ligaments. To overcome all of these disadvantages, orthoses have emerged as important medical devices to help patients in rehabilitation, as well as for self-care of deficiencies in clinics and daily life...
February 20, 2024: Sensors
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JOURNAL ARTICLE
Paulo Fernandes, Rui Pinto, Cláudia Correia, Isabel Mourão, Luísa Moura, Luis Miguel Brito
Kiwi waste from the calibration process is a major environmental problem of kiwi production due to landfill deposition. This work aims to contribute to the agronomic use of recycled kiwi waste through composting. With this objective, a composting experiment was carried out with kiwi fruit waste mixed with 5%, 10% and 20% (fresh weight) of wheat straw from bundles used to protect kiwifruit trunks from frost, as abulking agent to increase aeration, in the piles 5S, 10S and 20S, respectively. The highest temperatures for piles 5S and 10S were above 60°C, whereas the temperature did not reach 40°C in the pile with the highest straw content (20S) because the aeration increased heat loss in addition to increased C/N ratio of this pile...
March 12, 2024: Environmental Technology
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JOURNAL ARTICLE
José Lentilhas-Graça, Diogo J Santos, João Afonso, Andreia Monteiro, Andreia G Pinho, Vera M Mendes, Marta S Dias, Eduardo D Gomes, Rui Lima, Luís S Fernandes, Fernando Fernandes-Amorim, Inês M Pereira, Nídia de Sousa, Jorge R Cibrão, Aline M Fernandes, Sofia C Serra, Luís A Rocha, Jonas Campos, Tiffany S Pinho, Susana Monteiro, Bruno Manadas, António J Salgado, Ramiro D Almeida, Nuno A Silva
INTRODUCTION: The inflammatory response after spinal cord injury (SCI) is an important contributor to secondary damage. Infiltrating macrophages can acquire a spectrum of activation states, however, the microenvironment at the SCI site favors macrophage polarization into a pro-inflammatory phenotype, which is one of the reasons why macrophage transplantation has failed. METHODS: In this study, we investigated the therapeutic potential of the macrophage secretome for SCI recovery...
2024: Frontiers in Immunology
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JOURNAL ARTICLE
Rui D V Fernandes, Alina Pranovich, Sergiy Valyukh, Andrea Zille, Tomas Hallberg, Kenneth Järrendahl
Poly(styrene-methyl methacrylate-acrylic acid) photonic crystals (PCs), with five different sizes (170, 190, 210, 230 and 250 nm), were applied onto three plain fabrics, namely polyamide, polyester and cotton. The PC-coated fabrics were analyzed using scanning electronic microscopy and two UV/Vis reflectance spectrophotometric techniques (integrating sphere and scatterometry) to evaluate the PCs' self-assembly along with the obtained spectral and colors characteristics. Results showed that surface roughness of the fabrics had a major influence on the color produced by PCs...
January 25, 2024: Biomimetics
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JOURNAL ARTICLE
Camilo Mosquera, Marina Morante Silva, Ashleigh M Weyh, Michael A Malik, Rui Fernandes
STUDY DESIGN: Retrospective cohort study. OBJECTIVE: Malnutrition has been found to have negative effects on the immune system and inflammatory responses, impairing the wound healing process. Free flap failure is a serious complication in patients undergoing microvascular reconstruction, as it increases patient morbidity, length of stay in the hospital, patient, and hospital costs, as well as causes the need for further surgical interventions1 . Malnutrition is estimated to be present in 35-50% of head and neck cancer patients with higher rates in those experiencing hypo-oropharyngeal disease...
March 2024: Craniomaxillofacial Trauma & Reconstruction
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JOURNAL ARTICLE
Elisabete Teixeira, Cláudia Fernandes, Maria Bungărdean, Arnaud Da Cruz Paula, Raquel T Lima, Rui Batista, João Vinagre, Manuel Sobrinho-Simões, Valdemar Máximo, Paula Soares
In a family with Familial Non-Medullary Thyroid Carcinoma (FNMTC), our investigation using Whole-Exome Sequencing (WES) uncovered a novel germline USP42 mutation [ p.(Gly486Arg) ]. USP42 is known for regulating p53, cell cycle arrest, and apoptosis, and for being reported as overexpressed in breast and gastric cancer patients. Recently, a USP13 missense mutation was described in FNMTC, suggesting a potential involvement in thyroid cancer. Aiming to explore the USP42 mutation as an underlying cause of FNMTC, our team validated the mutation in blood and tissue samples from the family...
January 26, 2024: International Journal of Molecular Sciences
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JOURNAL ARTICLE
Maria R Pinheiro, Luís E Fernandes, Isa C Carneiro, Sónia D Carvalho, Rui M Henrique, Valery V Tuchin, Hélder P Oliveira, Luís M Oliveira
With the objective of developing new methods to acquire diagnostic information, the reconstruction of the broadband absorption coefficient spectra (μa [λ]) of healthy and chromophobe renal cell carcinoma kidney tissues was performed. By performing a weighted sum of the absorption spectra of proteins, DNA, oxygenated, and deoxygenated hemoglobin, lipids, water, melanin, and lipofuscin, it was possible to obtain a good match of the experimental μa (λ) of both kidney conditions. The weights used in those reconstructions were estimated using the least squares method, and assuming a total water content of 77% in both kidney tissues, it was possible to calculate the concentrations of the other tissue components...
February 6, 2024: Journal of Biophotonics
#14
JOURNAL ARTICLE
Duarte Freitas, António Antunes, Martine Thomis, Ricardo Silva, Gonçalo Marques, Alípio Silva, Rui Nunes, Marcelo Delgado, Paulo Jardim, Rubina Xíxaro, Joana Silva, Francisco Fernandes, Robert M Malina
BACKGROUND: Motor performances of youth are related to growth and maturity status, among other factors. AIM: To estimate the contribution of skeletal maturity status per se to the motor performances of female athletes aged 10-15 years and the mediation effects of growth status on the relationships. SUBJECTS AND METHODS: Skeletal age (TW3 RUS SA), body size, proportions, estimated fat-free mass (FFM), motor performances, training history and participation motivation were assessed in 80 non-skeletally mature female participants in several sports...
February 2024: Annals of Human Biology
#15
JOURNAL ARTICLE
Carla Barbosa, Alberta Araújo, Paulo Fernandes, Alexandre Romão, Manuel Rui Alves
To reduce the microbial load in goat's milk, which is less thermally stable than cow's milk, an alternative processing method was used in this study. This involved treating the milk with pulsed electric fields (PEFs) (at 10 kV·cm-1 , with 50 µs pulses for 3 Hz) and then heat-treating it at 63 °C for 6.0 s, as well as using heat treatment alone at 75 °C for 3.4 s. Cheeses were made using both types of milk treatment, and samples were collected after 5, 15, and 25 days of ripening for DNA extraction and purification, followed by high-throughput sequencing on the MiSeq Illumina sequencing platform...
November 21, 2023: Foods (Basel, Switzerland)
#16
JOURNAL ARTICLE
Wafaa Saleh, Hamad Alharbi, Sijia Yue, Rui P Fernandes
BACKGROUND: Lichen planus is one of the common adverse reactions after COVID-19 infection and vaccination. Despite it being reported in several case reports, the literature including a large sample of the studied population is lacking. The current study was performed to assess the risk of LP after COVID-19 infection as well as COVID-19 vaccination. METHODS: The current study was designed as a retrospective cross-sectional hospital-based study of registered patients at the University of Florida (UF) health centers...
December 8, 2023: Oral Diseases
#17
JOURNAL ARTICLE
Ana Oliveira, Luísa C Rodrigues, Diana Soares da Costa, Emanuel M Fernandes, Rui L Reis, Nuno M Neves, Pedro Leão, Albino Martins
Selective COX-2 inhibitors such as etoricoxib (ETX) are potentially indicated for the treatment of intestinal inflammatory disorders. However, their systemic administration provokes some off-site secondary effects, decreasing the desirable local effectiveness. To circumvent such limitations, herein an ETX delivery system based on electrospun fibrous meshes (eFMs) was proposed. ETX at different concentrations (1, 2, and 3 mg mL-1 ) was loaded into eFMs, which not affect the morphology and the mechanical properties of this drug delivery system (DDS)...
November 29, 2023: Biomater Adv
#18
JOURNAL ARTICLE
Rui Valdiviesso, Teresa F Amaral, Emília Moreira, Ana Rita Sousa-Santos, Mário Fernandes, Maria J V Aguiar, Sónia Martins, Luís F Azevedo, Lia Fernandes, José Silva-Cardoso, Nuno Borges
BACKGROUND: Frailty and sarcopenia have been extensively studied in heart failure (HF) patients, but their coexistence is unknown. The aim of this work is to describe the coexistence of these conditions in a sample of HF outpatients and its association with the use of medication and left-ventricular ejection fraction. METHODS: Participants in this cross-sectional study were recruited from a HF outpatients' clinic in northern Portugal. Frailty phenotype was assessed according to Fried et al...
December 5, 2023: BMC Cardiovascular Disorders
#19
REVIEW
Júlio Belo Fernandes, Noélia Ferreira, Josefa Domingos, Rui Ferreira, Catarina Amador, Nelson Pardal, Cidália Castro, Aida Simões, Sónia Fernandes, Catarina Bernardes, Diana Alves Vareta, Dina Peças, Dora Ladislau, Natacha Sousa, Andreia Duarte, Catarina Godinho
Patients with lower limb fractures require rehabilitation but often struggle with adherence to interventions. Adding motivational strategies to rehabilitation programs can increase patient adherence and enhance outcomes. This review aims to identify the motivational strategies used by health professionals in the rehabilitation of people with lower limb fractures. We used Arksey and O'Malley's methodological framework to structure and conduct this scoping review. The literature search was performed using the Scopus, CINAHL, MEDLINE, Nursing & Allied Health, and Cochrane Central Register of Controlled Trials databases...
November 10, 2023: International Journal of Environmental Research and Public Health
#20
REVIEW
Camilo Mosquera, Ashleigh Weyh, Michael Malik, Rui Fernandes, Anthony Bunnell, Stacey Nedrud
BACKGROUND: Radial forearm free flap (RFFF) donor site closure is traditionally performed with split thickness skin grafts (STSG), which can be associated with poor aesthetics, wrist stiffness, paresthesia, reduced strength, and tendon exposure. Full thickness skin grafts (FTSG) are potentially beneficial as they provide a more durable coverage, and the skin graft donor site can be closed primarily, which is more aesthetic. The aim of this systematic review is to compare the outcomes of STSG versus FTSG for closure of the RFFF donor site...
November 22, 2023: Microsurgery
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