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Methyl cyclodextrin

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https://www.readbyqxmd.com/read/28522425/development-and-characterization-of-fast-dissolving-tablets-of-oxaprozin-based-on-hybrid-systems-of-the-drug-with-cyclodextrins-and-nanoclays
#1
Francesca Maestrelli, Paola Mura, Marzia Cirri, Natascia Mennini, Carla Ghelardini, Lorenzo Di Cesare Mannelli
Previous studies highlighted an increase of the randomly-methylated-ß-cyclodextrin (RAMEB) solubilizing power towards oxaprozin when used in combination with L-arginine (ARG) or sepiolite nanoclay (SV). Therefore, the aim of this work was to investigate the possibility of maximising the RAMEB solubilizing efficacy by a joined approach based on the entrapment in SV of the drug-RAMEB-ARG complex. The quaternary nanocomposite was prepared by different techniques and characterized for solid state and dissolution properties, compared to ternary drug combinations with RAMEB-ARG, RAMEB-SV or ARG-SV...
May 15, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28513911/sperm-capacitation-pretreatment-positively-impacts-bovine-intracytoplasmic-sperm-injection
#2
L Águila, F Zambrano, M E Arias, Felmer R
The efficiency of intracytoplasmic sperm injection (ICSI) in bovines is low compared to other species due in part to inadequate egg activation and sperm nucleus decondensation after injection. We hypothesized that this low efficiency is due to the lack of complete sperm capacitation, so we evaluated the effects of isobutylmethylxanthine (IBMX) and methyl-β-cyclodextrin (MβCD) on bovine sperm capacitation and on the preimplantation developmental potential of bovine embryos generated by ICSI. Treatment with IBMX and MβCD decreased sperm viability (between 13 to 30%); nevertheless, 0...
May 17, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28510132/methyl-%C3%AE-cyclodextrin-modulates-thapsigargin-induced-store-dependent-ca-2-entry-in-macrophages
#3
Z I Krutetskaya, L S Milenina, A A Naumova, S N Butov, V G Antonov, A D Nozdrachev
Using Fura-2AM microfluorimetry, we have shown for the first time that preincubation of macrophages with methyl-β-cyclodextrin, inducing cholesterol extraction from membranes and raft disruption, leads to significant inhibition of thapsigargin-induced store-dependent Ca(2+) entry in rat peritoneal macrophages. In contrast, macrophage treatment with methyl-β-cyclodextrin after Ca(2+) entry mechanisms were activated by store depletion by thapsigargin application leads to potentiation of subsequent store-dependent Ca(2+) entry...
March 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28504140/dft-study-on-the-dissolution-mechanisms-of-%C3%AE-cyclodextrin-and-chitobiose-in-ionic-liquid
#4
Bobo Cao, Jiuyao Du, Ziping Cao, Xuejun Sun, Haitao Sun, Hui Fu
Density functional theory (DFT) was employed to study the dissolution mechanisms of α-cyclodextrin and chitobiose in 1-ethyl-3-methyl-imidazolium acetate ([Emim][OAc]). Geometrical analysis of the studied complexes indicated that both anion and cation in ionic liquid interacting withα-cyclodextrin and chitobiose contributed to the dissolution reaction. Intermolecular interactions in the complexes were identified as non-covalent interactions, such as hydrogen bonds, van der Waals interactions and repulsions, which were considered as the driving force of dissolution...
August 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28496320/induction-of-mitophagy-mediated-antitumor-activity-with-folate-appended-methyl-%C3%AE-cyclodextrin
#5
Kazuhisa Kameyama, Keiichi Motoyama, Nao Tanaka, Yuki Yamashita, Taishi Higashi, Hidetoshi Arima
Mitophagy is the specific autophagic elimination system of mitochondria, which regulates cellular survival via the removal of damaged mitochondria. Recently, we revealed that folate-appended methyl-β-cyclodextrin (FA-M-β-CyD) provides selective antitumor activity in folate receptor-α (FR-α)-expressing cells by the induction of autophagy. In this study, to gain insight into the detailed mechanism of this antitumor activity, we focused on the induction of mitophagy by the treatment of FR-α-expressing tumor cells with FA-M-β-CyD...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28485208/the-distribution-of-vinculin-to-lipid-rafts-plays-an-important-role-in-sensing-stiffness-of-extracellular-matrix
#6
Ayaka Ichikawa Nagasato, Hiroshi Yamashita, Michinori Matsuo, Kazumitsu Ueda, Noriyuki Kioka
Extracellular matrix (ECM) stiffness regulates cell differentiation, survival, and migration. Our previous study has shown that the interaction of the focal adhesion protein vinculin with vinexin α plays a critical role in sensing ECM stiffness and regulating stiffness-dependent cell migration. However, the mechanism how vinculin-vinexin α interaction affects stiffness-dependent cell migration is unclear. Lipid rafts are membrane microdomains that are known to affect ECM-induced signals and cell behaviors...
June 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28483280/stacking-cyclodextrin-microchip-electrokinetic-chromatographic-determination-of-gabapentinoid-drugs-in-pharmaceutical-and-biological-matrices
#7
Abdallah M Zeid, Noritada Kaji, Jenny Jeehan M Nasr, Fathalla F Belal, Yoshinobu Baba, Mohamed I Walash
A facile, rapid, and highly sensitive microchip-based electrokinetic chromatographic method was developed for the simultaneous analysis of two gabapentinoid drugs, gabapentin (GPN) and pregabalin (PGN). Both drugs were first reacted with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) via nucleophilic substitution reactions to yield highly fluorescent products with λex/em 470/540nm. Analyses of both fluorescently labeled compounds were achieved within 200s in a poly(methyl methacrylate) (PMMA) microchip with a 30mm separation channel...
April 26, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28447973/methods-to-study-lipid-alterations-in-neutrophils-and-the-subsequent-formation-of-neutrophil-extracellular-traps
#8
Graham Brogden, Ariane Neumann, Diab M Husein, Friederike Reuner, Hassan Y Naim, Maren von Köckritz-Blickwede
Lipid analysis performed by high performance thin layer chromatography (HPTLC) is a relatively simple, cost-effective method of analyzing a broad range of lipids. The function of lipids (e.g., in host-pathogen interactions or host entry) has been reported to play a crucial role in cellular processes. Here, we show a method to determine lipid composition, with a focus on the cholesterol level of primary blood-derived neutrophils, by HPTLC in comparison to high performance liquid chromatography (HPLC). The aim was to investigate the role of lipid/cholesterol alterations in the formation of neutrophil extracellular traps (NETs)...
March 29, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28446672/cellular-cholesterol-facilitates-the-post-entry-replication-cycle-of-herpes-simplex-virus-1
#9
George A Wudiri, Anthony V Nicola
Cholesterol is an essential component of cell membranes and is required for HSV-1 entry (1-3). Treatment of HSV-1-infected Vero cells with methyl beta-cyclodextrin from 2 - 9 hours post-entry reduced plaque numbers. Transport of incoming viral capsids to the nuclear periphery was unaffected by cholesterol reduction suggesting that cell cholesterol is important for the HSV-1 replicative cycle at a stage(s) beyond entry after the arrival of capsids at the nucleus. The synthesis and release of infectious HSV-1 and cell-to-cell spread of infection were all impaired in cholesterol-reduced cells...
April 26, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28445756/membrane-mechanics-of-primary-afferent-neurons-in-the-dorsal-root-ganglia-of-rats
#10
Hirosato Kanda, Jianguo G Gu
Membrane mechanics is an important biological factor regulating many cellular functions including cell motility, intercellular and intracellular signaling, gene expression, and membrane ion channel activity. Primary afferent neurons transduce sensory information about temperature, touch, and pain. These sensory functions may be profoundly affected by the states of primary afferent neuron mechanics. However, membrane mechanics of primary afferent neurons is largely unknown. In this study, we established the optical trapping technique for determining membrane mechanics of cultured primary afferent neurons of the dorsal root ganglia (DRG)...
April 25, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28432770/chiral-separation-of-aryloxyphenoxy-propionate-herbicides-in-a-permethyl-%C3%AE-cyclodextrin-based-column-influence-of-temperature-and-mobile-phase-composition-on-enantioselectivity
#11
Ester Lubomirsky, Juan M Padró, Héctor Di Loreto, Cecilia B Castells
We used a permethyl-β-cyclodextrin chiral stationary phase under reversed-phase conditions for the chiral separation of four aryloxyphenoxy-propionate herbicides (fenoxaprop-p-ethyl, quizalofop-p-ethyl and tefuryl, and haloxyfop-p-methyl) with mixtures of methanol, ethanol, 2-propanol, n-propanol, tert-butanol, or acetonitrile and water as mobile phases and investigated the influence of mobile phase composition and column temperature (from 0 to 50°C) on the separation. The retention factors (k) and selectivity factors (α) of all the herbicides investigated decreased with increasing temperature...
April 22, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28423596/transient-activation-of-the-pi3k-akt-pathway-promotes-newcastle-disease-virus-replication-and-enhances-anti-apoptotic-signaling-responses
#12
Yinfeng Kang, Runyu Yuan, Xiaqiong Zhao, Bin Xiang, Shimin Gao, Pei Gao, Xu Dai, Minsha Feng, Yanling Li, Peng Xie, Yulian Li, Xiaoyi Gao, Tao Ren
Viral infection activates a host's cellular phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway, which is involved in cell differentiation, growth, survival, and apoptosis. To elucidate molecular mechanisms in the pathogenesis of Newcastle disease virus (NDV), we demonstrated that NDV transiently activates the PI3K/Akt pathway in chicken cells at an early phase of infection. Its activation was observed as early as 15 min post-infection and gradually weakened after 24 h. Incubating cells with a PI3K inhibitor, LY294002 or wortmannin, prior to NDV infection decreased NDV progeny yields and suppressed Akt phosphorylation at early times post-infection...
April 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28421241/effect-of-methyl-%C3%AE-cyclodextrin-on-gene-expression-in-microbial-conversion-of-phytosterol
#13
Victoria Y Shtratnikova, Mikhail I Schelkunov, Dmitry V Dovbnya, Eugeny Y Bragin, Marina V Donova
Modified β-cyclodextrins are widely used for the enhancement of microbial conversions of lipophilic compounds such as steroids. Multiple mechanisms of cyclodextrin-mediated enhancement of phytosterol bioconversion by mycobacteria had previously been shown to include steroid solubilization, alterations in the cell wall permeability for both steroids and nutrients, facilitation of protein leaking, and activity suppression of some steroid-transforming enzymes.In this work, we studied whether cyclodextrins might affect expression of the genes involved in the steroid catabolic pathway...
April 18, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28412819/structural-and-spectroscopic-characterization-of-tpgs-micelles-disruptive-role-of-cyclodextrins-and-kinetic-pathways
#14
Joan Puig-Rigall, Isabelle Grillo, Cécile A Dreiss, Gustavo González-Gaitano
The aggregation and structure of d-α-tocopheryl polyethylene glycol succinate micelles, TPGS-1000, an amphiphilic derivative of vitamin E, were characterized using scattering and spectroscopic methods, and the impact of different cyclodextrins (CDs) on the self-assembly was investigated, with the view of combining these two versatile pharmaceutical excipients in drug formulations. Combined small-angle neutron scattering (SANS), dynamic light scattering, and time-resolved and steady-state fluorescence emission experiments revealed a core-shell architecture with a high aggregation number (Nagg ≈ 100) and a highly hydrated poly(ethylene oxide) corona (∼11 molecules of solvent per ethylene oxide unit)...
May 2, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28411453/inhibition-of-influenza-virus-infection-by-multivalent-pentacyclic-triterpene-functionalized-per-o-methylated-cyclodextrin-conjugates
#15
Zhenyu Tian, Longlong Si, Kun Meng, Xiaoshu Zhou, Yongmin Zhang, Demin Zhou, Sulong Xiao
Multivalent ligands that exhibit high binding affinity to influenza hemagglutinin (HA) trimer can block the interaction of HA with its sialic acid receptor. In this study, a series of multivalent pentacyclic triterpene-functionalized per-O-methylated cyclodextrin (CD) derivatives were designed and synthesized using 1, 3-dipolar cycloaddition click reaction. A cell-based assay showed that three compounds (25, 28 and 31) exhibited strong inhibitory activity against influenza A/WSN/33 (H1N1) virus. Compound 28 showed the most potent anti-influenza activity with IC50 of 4...
April 2, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28406307/%C3%AE-cyclodextrin-as-a-catalyst-for-the-synthesis-of-2-methyl-3-5-diarylisoxazolidines-in-water
#16
Giuseppe Floresta, Carmen Talotta, Carmine Gaeta, Margherita De Rosa, Ugo Chiacchio, Placido Neri, Antonio Rescifina
A green and efficient 1,3-dipolar cycloaddition of nitrones with different styrenes and cinnamates using a catalytic amount of γ-cyclodextrin (γ-CD) in water has been developed to give substituted isoxazolidines. γ-CD was found to be highly efficacious in carrying out this reaction under an eco-friendly environment, affording moderate to excellent yields and, in some cases, excellent diastereomeric excess (up to >95%) at 100 °C in 8-12 h. The catalyst can be easily recuperated and recycled for several times without loss of activity...
May 5, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28394556/%C3%AE-cd-functionalized-microdevice-for-rapid-capture-and-release-of-bacteria
#17
Alexandra Perez-Anes, Anna Szarpak-Jankowska, Dorothée Jary, Rachel Auzély-Velty
Most procedures for detecting pathogens in liquid media require an initial concentration step. In this regard, carbohydrates have proven to be attractive affinity ligands for the solid-phase capture of bacteria that use lectins for adhesion to host cell membranes. However, the use of cyclodextrin-immobilized substrates to selectively trap bacteria has not been explored before. Here, using quartz-crystal microbalance with dissipation monitoring experiments, we demonstrate that functionalization of surfaces by β-cyclodextrin (β-CD) can not only allow for rapid and efficient capture of bacterial cells in liquid but also their facile elution with an aqueous solution of a selectively methylated β-CD derivative as a competitive molecule...
April 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28388069/detection-and-removal-of-endogenous-carbon-monoxide-by-selective-and-cell-permeable-hemoprotein-model-complexes
#18
Saika Minegishi, Aki Yumura, Hirotsuna Miyoshi, Shigeru Negi, Shigeru Taketani, Roberto Motterlini, Roberta Foresti, Koji Kano, Hiroaki Kitagishi
Carbon monoxide (CO) is produced in mammalian cells during heme metabolism and serves as an important signaling messenger. Here we report the bioactive properties of selective CO scavengers, hemoCD1 and its derivative R8-hemoCD1, which have the ability to detect and remove endogenous CO in cells. HemoCD1 is a supramolecular hemoprotein-model complex composed of 5,10,15,20-tetrakis(4-sulfonatophenyl)porphinatoiron(II) and a per-O-methylated β-cyclodextrin dimer having an pyridine linker. We demonstrate that hemoCD1 can be used effectively to quantify endogenous CO in cell lysates by a simple spectrophotometric method...
April 17, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28384320/direct-administration-of-2-hydroxypropyl-beta-cyclodextrin-into-guinea-pig-cochleae-effects-on-physiological-and-histological-measurements
#19
J T Lichtenhan, K Hirose, C A Buchman, R K Duncan, A N Salt
2-Hydroxypropyl-Beta-Cyclodextrin (HPβCD) can be used to treat Niemann-Pick type C disease, Alzheimer's disease, and atherosclerosis. But, a consequence is that HPβCD can cause hearing loss. HPβCD was recently found to be toxic to outer hair cells (OHCs) in the organ of Corti. Previous studies on the chronic effects of in vivo HPβCD toxicity did not know the intra-cochlear concentration of HPβCD and attributed variable effects on OHCs to indirect drug delivery to the cochlea. We studied the acute effects of known HPβCD concentrations administered directly into intact guinea pig cochleae...
2017: PloS One
https://www.readbyqxmd.com/read/28381673/iron-ii-porphyrin-cyclodextrin-supramolecular-complex-as-a-carbon-monoxide-depleting-agent-in-living-organisms
#20
REVIEW
Hiroaki Kitagishi, Saika Minegishi
The specific intermolecular interaction between an anionic tetraarylporphyrin and per-O-methylated β-cyclodextrin (TMe-β-CD) paved the way to produce a functional supramolecule that works as a strong carbon monoxide (CO)-depleting agent in living organisms. The supramolecular complex, hemoCD, that is composed of meso-tetrakis(4-sulfonatophenyl)porphinatoiron(II) and a TMe-β-CD dimer linked by a pyridine linker, captured internal CO from carboxyhemoglobin during its circulation in the blood of animals. HemoCD thus produced the pseudo-knockdown (loss-of-functional) state of endogenous CO in the animals...
2017: Chemical & Pharmaceutical Bulletin
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