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Methyl cyclodextrin

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https://www.readbyqxmd.com/read/29350528/proton-and-carbon-13-dynamic-nuclear-polarization-of-methylated-%C3%AE-cyclodextrins
#1
Filippo Caracciolo, Alessandra Lucini Paioni, Lucio Melone, Marta Filibian, Pietro Carretta
1 H and 13 C dynamic nuclear polarization has been studied in 13 C-enriched β - cyclodextrins doped with TEMPO free radical. 1 H and 13 C polarizations raised above 7.5 % and 7 % , respectively, and for both nuclear species the transfer of polarization from the electron spins appears to be consistent with a thermal mixing scenario for a concentration of 9 13 C nuclei per molecule. When the concentration is increased to 21 13 C nuclei per molecule a decrease in the spin-lattice relaxation and polarization build up rates is observed...
January 19, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29338971/atorvastatin-impaired-glucose-metabolism-in-c2c12-cells-partly-via-inhibiting-cholesterol-dependent-glucose-transporter-4-translocation
#2
Binbin Sun, Zeyu Zhong, Fan Wang, Jiong Xu, Feng Xu, Weimin Kong, Zhaoli Ling, Nan Shu, Ying Li, Tong Wu, Mian Zhang, Liang Zhu, Xiaodong Liu, Li Liu
Skeletal muscle accounts for approximately 75% of glucose disposal in body and statins impair glucose metabolism. We aimed to investigate the effect of atorvastatin on glucose metabolism in C2C12 cells. Glucose metabolism and expression of glucose transporter 4 (GLUT4) and hexokinase II (HXKII) were measured following incubation with atorvastatin or pravastatin. Roles of cholesterol in atorvastatin-induced glucose metabolism impairment were investigated via adding cholesterol or mevalonic acid and confirmed by cholesterol depletion with methyl-β-cyclodextrin...
January 12, 2018: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29336193/enhancement-of-iodinin-solubility-by-encapsulation-into-cyclodextrin-nanoparticles
#3
Anthony Prandina, Lars Herfindal, Sylvie Radix, Pål Rongved, Stein O Døskeland, Marc Le Borgne, Florent Perret
Phenazine is known to regroup planar nitrogen-containing heterocyclic compounds. It was used here to enhance the bioavailability of the biologically important compound iodinin, which is near insoluble in aqueous solutions. Its water solubility has led to the development of new formulations using diverse amphiphilic α-cyclodextrins (CDs). With the per-[6-desoxy-6-(3-perfluorohexylpropanethio)-2,3-di-O-methyl]-α-CD, we succeeded to get iodinin-loaded nanoformulations with good parameters such as a size of 97...
December 2018: Journal of Enzyme Inhibition and Medicinal Chemistry
https://www.readbyqxmd.com/read/29304557/abnormal-golgi-ph-homeostasis-in-cancer-cells-impairs-apical-targeting-of-carcinoembryonic-antigen-ceacam5-by-inhibiting-its-gpi-anchor-mediated-association-with-lipid-rafts
#4
Nina Kokkonen, Elham Khosrowabadi, Antti Hassinen, Deborah Harrus, Tuomo Glumoff, Thomas Kietzmann, Sakari Kellokumpu
Carcinoembryonic antigen (CEACAM5, CEA) is a known tumor marker for colorectal cancer that localizes in a polarized manner to the apical surface in normal colon epithelial cells while in cancer cells it is present at both the apical and basolateral surfaces of the cells. Since the Golgi apparatus sorts and transports most proteins to these cell surface domains, we set out here to investigate whether any of the factors commonly associated with tumorigenesis including hypoxia, generation of reactive oxygen species (ROS), altered redox homeostasis or an altered Golgi pH, are responsible for mistargeting of CEA to the basolateral surface in cancer cells...
January 5, 2018: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/29286881/daurichromenic-acid-and-grifolic-acid-phytotoxic-meroterpenoids-that-induce-cell-death-in-cell-culture-of-their-producer-rhododendron-dauricum
#5
Futoshi Taura, Miu Iijima, Fumiya Kurosaki
Daurichromenic acid (DCA) is a meroterpenoid with anti-HIV activities that is isolated from Rhododendron dauricum L. We recently reported that DCA is biosynthesized and accumulated in the apoplast of glandular scales attached on the surface of young leaves of R. dauricum. In the present study, we confirmed that a cell suspension culture of R. dauricum could not produce DCA and its precursor grifolic acid even after elicitation with methyl jasmonate and b-cyclodextrin. In addition, exogenous supplementation of DCA and grifolic acid effectively induced cell death in the same culture, with apoptosis-associated phenomena such as cytoplasmic shrinkage, chromatin condensation, and genomic DNA degradation...
December 29, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/29286295/determination-of-tangeretin-in-rat-plasma-assessment-of-its-clearance-and-absolute-oral-bioavailability
#6
Mai Gamal Elhennawy, Hai-Shu Lin
Tangeretin (TAN) is a dietary polymethoxylated flavone that possesses a broad scope of pharmacological activities. A simple high-performance liquid chromatography (HPLC) method was developed and validated in this study to quantify TAN in plasma of Sprague-Dawley rats. The lower limit of quantification (LLOQ) was 15 ng/mL; the intra- and inter-day assay variations expressed in the form of relative standard deviation (RSD) were all less than 10%; and the assay accuracy was within 100 ± 15%. Subsequently, pharmacokinetic profiles of TAN were explored and established...
December 29, 2017: Pharmaceutics
https://www.readbyqxmd.com/read/29279104/cross-linked-chitosan-%C3%AE-cyclodextrin-composite-for-selective-removal-of-methyl-orange-adsorption-performance-and-mechanism
#7
Yezhou Jiang, Bingchuan Liu, Jikun Xu, Keliang Pan, Huijie Hou, Jingping Hu, Jiakuan Yang
A complex chitosan/β-cyclodextrin polymer was synthesized by bridging with maleoyl chains followed by cross-linking with glutaraldehyde. The adsorption performance of the synthetic polymer was investigated for selective removal of methyl orange (MO) from aqueous solution. The kinetic behavior was well fitted by the pseudo-second order model, while the adsorption process at equilibrium followed the Langmuir isotherm model. The thermodynamic parameters suggested that the adsorption was exothermic and spontaneous...
February 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29277655/phosphatidylinositol-4-5-bisphosphate-cholesterol-and-fatty-acids-modulate-the-calcium-activated-chloride-channel-tmem16a-ano1
#8
José J De Jesús-Pérez, Silvia Cruz-Rangel, Ángeles E Espino-Saldaña, Ataúlfo Martínez-Torres, Zhiqiang Qu, H Criss Hartzell, Nancy E Corral-Fernandez, Patricia Pérez-Cornejo, Jorge Arreola
The TMEM16A-mediated Ca2+-activated Cl- current drives several important physiological functions. Membrane lipids regulate ion channels and transporters but their influence on members of the TMEM16 family is poorly understood. Here we have studied the regulation of TMEM16A by phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), cholesterol, and fatty acids using patch clamp, biochemistry and fluorescence microscopy. We found that depletion of membrane PI(4,5)P2 causes a decline in TMEM16A current that is independent of cytoskeleton, but is partially prevented by removing intracellular Ca2+...
December 22, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29273450/helicobacter-pylori-depletes-cholesterol-in-gastric-glands-to-prevent-interferon-gamma-signaling-and-escape-the-inflammatory-response
#9
Pau Morey, Lennart Pfannkuch, Ervinna Pang, Francesco Boccellato, Michael Sigal, Aki Imai-Matsushima, Victoria Dyer, Manuel Koch, Hans-Joachim Mollenkopf, Philipp Schlaermann, Thomas F Meyer
BACKGROUND & AIMS: Despite inducing an inflammatory response, Helicobacter pylori can persist in the gastric mucosa for decades. H pylori expression of cholesterol-α-glucosyltransferase (encoded by cgt) is required for gastric colonization and T-cell activation. We investigated how cgt affects gastric epithelial cells and the host immune response. METHODS: MKN45 gastric epithelial cells, AGS cells, and human primary gastric epithelial cells (obtained from patients undergoing gastrectomy or sleeve resection or gastric antral organoids) were incubated with interferon gamma (IFNG) or IFNB and exposed to H pylori, including cagPAI and cgt mutant strains...
December 19, 2017: Gastroenterology
https://www.readbyqxmd.com/read/29244868/cyclodextrin-containing-hydrogels-as-an-intraocular-lens-for-sustained-drug-release
#10
Xiao Li, Yang Zhao, Kaijie Wang, Lei Wang, Xiaohui Yang, Siquan Zhu
To improve the efficacy of anti-inflammatory factors in patients who undergo cataract surgery, poly(2-hydroxyethyl methacrylate-co-methyl methacrylate) (p(HEMA-co-MMA)) hydrogels containing β-cyclodextrin (β-CD) (pHEMA/MMA/β-CD) were designed and prepared as intraocular lens (IOLs) biomaterials that could be loaded with and achieve the sustained release of dexamethasone. A series of pHEMA/MMA/β-CD copolymers containing different ratios of β-CD (range, 2.77 to 10.24 wt.%) were obtained using thermal polymerization...
2017: PloS One
https://www.readbyqxmd.com/read/29241586/influence-of-membrane-cholesterol-on-monocyte-chemotaxis
#11
Amit K Saha, Marzieh Mousavi, Shatha F Dallo, Shankar J Evani, Anand K Ramasubramanian
Cholesterol content influences several important physiological functions due to its effect on membrane receptors. In this work, we tested the hypothesis that cellular cholesterol alters chemotactic response of monocytes to Monocyte Chemoattractant Protein-1 (MCP-1) due to their effect on the receptor, CCR2. We used Methyl-β-cyclodextrin (MβCD) to alter the baseline cholesterol in human monocytic cell line THP-1, and evaluated their chemotactic response to MCP-1. Compared to untreated cells, cholesterol enrichment increased the number of monocytes transmigrated in response to MCP-1 while depletion had opposite effect...
December 9, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/29235455/atmospheric-pressure-mass-spectrometric-imaging-of-live-hippocampal-tissue-slices-with-subcellular-spatial-resolution
#12
Jae Young Kim, Eun Seok Seo, Hyunmin Kim, Ji-Won Park, Dong-Kwon Lim, Dae Won Moon
We report a high spatial resolution mass spectrometry (MS) system that allows us to image live hippocampal tissue slices under open-air atmospheric pressure (AP) and ambient temperature conditions at the subcellular level. The method is based on an efficient desorption process by femtosecond (fs) laser assisted with nanoparticles and a subsequent ionization step by applying nonthermal plasma, termed AP nanoparticle and plasma assisted laser desorption ionization (AP-nanoPALDI) MS method. Combining the AP-nanoPALDI with microscopic sample scanning, MS imaging with spatial resolution of 2...
December 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/29214230/cytotoxicity-of-endocytosis-and-efflux-inhibitors-in-the-bewo-cell-line
#13
Mansi Shah, Luke Bourner, Shariq Ali, Sanaalarab Al-Enazy, Erik Rytting
Aims: The purpose of this study was to determine the cell viability and cytotoxicity of various endocytosis and efflux inhibitors which can be used to determine transport and uptake mechanisms in the BeWo (b30 clone) human placental trophoblast cell line. Ethanol and dimethylsulfoxide (DMSO) were also studied since they are often used as cosolvents for administration of these inhibitors. Methodology: The water-soluble tetrazolium-1 (WST-1) assay was used to quantify cell viability and the lactate dehydrogenase (LDH) assay was used to determine cytotoxicity...
2017: Journal of Pharmaceutical Research International
https://www.readbyqxmd.com/read/29213055/inhibiting-mevalonate-pathway-enzymes-increases-stromal-cell-resilience-to-a-cholesterol-dependent-cytolysin
#14
Sholeem Griffin, Giulio Preta, Iain Martin Sheldon
Animal health depends on the ability of immune cells to kill invading pathogens, and on the resilience of tissues to tolerate the presence of pathogens. Trueperella pyogenes causes tissue pathology in many mammals by secreting a cholesterol-dependent cytolysin, pyolysin (PLO), which targets stromal cells. Cellular cholesterol is derived from squalene, which is synthesized via the mevalonate pathway enzymes, including HMGCR, FDPS and FDFT1. The present study tested the hypothesis that inhibiting enzymes in the mevalonate pathway to reduce cellular cholesterol increases the resilience of stromal cells to PLO...
December 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29207048/tlr4-promotes-the-expression-of-hif-1%C3%AE-by-triggering-reactive-oxygen-species-in-cervical-cancer-cells-in%C3%A2-vitro-implications-for-therapeutic-intervention
#15
Xiao Yang, Gan Tao Chen, Yan Qing Wang, Shu Xian, Li Zhang, Shao Ming Zhu, Feng Pan, Yan Xiang Cheng
The present study investigated the mechanism underlying Toll-like receptor 4 (TLR4)-mediated stimulation of hypoxia-inducible factor-1α (HIF-1α) activity and its association with reactive oxygen species (ROS) in cervical cancer cells. SiHa cells were cultured and randomized to control, lipopolysaccharide (LPS), methyl-β-cyclodextrin (MβCD)+LPS, ammonium pyrrolidinedithiocarbamate (PDTC)+LPS, ST2825+LPS and small interfering (si) RNA TLR4+LPS treatment groups. Cell proliferation was quantified using an MTT assay, cell cloning was performed using soft agar colony formation and HIF-1α expression was detected by immunocytochemical staining and western blot analyses...
November 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29187674/nitrative-dna-damage-in-cultured-macrophages-exposed-to-indium-oxide
#16
Tahmina Afroz, Yusuke Hiraku, Ning Ma, Sharif Ahmed, Shinji Oikawa, Shosuke Kawanishi, Mariko Murata
OBJECTIVES: Indium compounds are used in manufacturing displays of mobile phones and televisions. However, these materials cause interstitial pneumonia in exposed workers. Animal experiments demonstrated that indium compounds caused lung cancer. Chronic inflammation is considered to play a role in lung carcinogenesis and fibrosis induced by particulate matters. 8-Nitroguanine (8-nitroG) is a mutagenic DNA lesion formed during inflammation and may participate in carcinogenesis. To clarify the mechanism of carcinogenesis, we examined 8-nitroG formation in indium-exposed cultured cells...
November 29, 2017: Journal of Occupational Health
https://www.readbyqxmd.com/read/29185164/extracellular-chromone-derivatives-in-cell-cultures-of-pimpinella-anisum-influence-of-elicitation-with-methyl-jasmonate-and-2%C3%AE-methyl-cyclodextrins
#17
Camilo Soto-Argel, Diego Hidalgo, Javier Palazon, Purificación Corchete
OBJECTIVES: To explore the potentiality of undifferentiated Pimpinella anisum L. cell cultures for the production of secondary metabolites by means of elicitation. RESULTS: Two chromone compounds were secreted to the medium of undifferentiated cultures of P. anisum: 4-methoxyfuro[3,2-g]chromen-7-one, known as bergapten, which is constitutive to anise, and 5-hydroxy-7-methoxy-2-methylchromen-4-one, the rare chromone eugenin, not yet described in P. anisum. Caffeoyl quinic acid species were also identified in the biomass...
November 28, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/29173029/enzymatically-synthesized-megalo-type-isomaltosaccharides-enhance-the-barrier-function-of-the-tight-junction-in-the-intestinal-epithelium
#18
Hiroshi Hara, Shunsuke Kume, Takahisa Iizuka, Yoshinori Fujimoto, Atsuo Kimura
Megalo-type isomaltosaccharides are an enzymatically synthesized foodstuff produced by transglucosylation from maltodextrin, and they contain a mid-chain length polymer of D-glucose with α-1,6-glycoside linkages. The injection of a solution of megalo-type isomaltosaccharides (1-4%(w/v), average DP = 12.6), but not oligo-type isomaltosaccharides (average DP = 3.3), into the intestinal lumen dose-dependently reduced the transport rates of tight junction permeable markers in a ligated loop of the anesthetized rat jejunum...
November 27, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/29162448/intracellular-cholesterol-level-regulates-sensitivity-of-glioblastoma-cells-against-temozolomide-induced-cell-death-by-modulation-of-caspase-8-activation-via-death-receptor-5-accumulation-and-activation-in-the-plasma-membrane-lipid-raft
#19
Yutaro Yamamoto, Arata Tomiyama, Nobuyoshi Sasaki, Hideki Yamaguchi, Takuya Shirakihara, Katsuhiko Nakashima, Kosuke Kumagai, Satoru Takeuchi, Terushige Toyooka, Kojiro Wada, Yoshitaka Narita, Koichi Ichimura, Ryuichi Sakai, Hiroki Namba, Kentaro Mori
Development of resistance against temozolomide (TMZ) in glioblastoma (GBM) after continuous treatment with TMZ is one of the critical problems in clinical GBM therapy. Intracellular cholesterol regulates cancer cell biology, but whether intracellular cholesterol is involved in TMZ resistance of GBM cells remains unclear. The involvement of intracellular cholesterol in acquired resistance against TMZ in GBM cells was investigated. Intracellular cholesterol levels were measured in human U251 MG cells with acquired TMZ resistance (U251-R cells) and TMZ-sensitive control U251 MG cells (U251-Con cells), and found that the intracellular cholesterol level was significantly lower in U251-R cells than in U251-Con cells...
November 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29156883/low-molecular-weight-supramolecular-ionogel-based-on-host-guest-interaction
#20
Aoli Wu, Fei Lu, Panpan Sun, Xuanxuan Qiao, Xinpei Gao, Li-Qiang Zheng
Supramolecular ionogels were prepared by self-assemble of small molecules through host-guest interaction between β-cyclodextrin (β-CD) and a room-temperature ionic liquid (IL) 3-(1-methyl-3-imidazolio)propanesulfonate - lithium bis(trifluoromethanesulfonyl)imide (MIPS-LiTFSI) which contains zwitterion MIPS. 19F NMR and 2D ROESY 1H NMR have been used to prove that only TFSI- is involved in the complexation. 1H NMR, FT-IR and comparative tests indicated that the electrostatic interaction between imidazole cation and TFSI- anion and intermolecular hydrogen bonding between three compounds also contribute to the formation of supramolecular ionogel...
November 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
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