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Methyl cyclodextrin

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https://www.readbyqxmd.com/read/28933537/membrane-fluidity-sensing-on-the-single-virus-particle-level-with-plasmonic-nanoparticle-transducers
#1
Amin Feizpour, David N Stelter, Crystal Wong, Hisashi Akiyama, Suryaram Gummuluru, Tom Keyes, Bjoern M Reinhard
Viral membranes are nanomaterials whose fluidity depends on their composition, in particular the cholesterol (chol) content. As differences in the membrane composition of individual virus particles can lead to different intracellular fates, biophysical tools capable of sensing the membrane fluidity on the single-virus level are required. In this manuscript, we demonstrate that fluctuations in the polarization of light scattered off gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs) encode information about the membrane fluidity of individual VLPs...
September 21, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28918640/supramolecular-interaction-assisted-fluorescence-and-tunable-stimuli-responsiveness-of-l-phenylalanine-based-polymers
#2
Mridula Nandi, Binoy Maiti, Kambalapalli Srikanth, Priyadarsi De
Supramolecular host-guest interaction between randomly methylated β-cyclodextrin (RM β-CD) and side-chain phenylalanine (Phe) and Phe-Phe dipeptide based homopolymers has been employed for amplification of fluorescence emission of otherwise weakly fluorescent amino acid Phe. The host-guest complex has been characterized by 1H and 13C NMR spectroscopy, two-dimensional (2D) rotating-frame overhauser spectroscopy (ROESY), FT-IR, UV-visible and fluorescence spectroscopy. To gain insights into the origin of fluorescence in homopolymers, density functional theory (DFT) calculations were performed where phenyl moieties inside the less polar core of β-CD were observed to form π-π coupled complex resulting in enhanced emission...
September 18, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28910399/cholesterol-added-prior-to-vitrification-on-the-cryotolerance-of-immature-and-in-vitro-matured-bovine-oocytes
#3
Núria Arcarons, Roser Morató, Meritxell Vendrell, Marc Yeste, Manel López-Bejar, Kosala Rajapaksha, Muhammad Anzar, Teresa Mogas
This study examines whether incorporating cholesterol-loaded methyl-β-cyclodextrin (CLC) in the bovine oocyte plasma membrane improves oocyte tolerance to vitrification. In vitro matured oocytes were incubated with 2 mg/ml BODIPY-labeled CLC for different time intervals in FCS or PVA supplemented medium or exposed to different CLC concentrations to examine the subcellular localization of cholesterol by confocal microscopy live-cell imaging. Subsequently, the effects of optimized CLC concentrations and incubation times prior to vitrification on early embryo development were assessed...
2017: PloS One
https://www.readbyqxmd.com/read/28884395/cellular-cholesterol-is-required-for-porcine-nidovirus-infection
#4
Ji Hyun Jeon, Changhee Lee
Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine epidemic diarrhea virus (PEDV) are porcine nidoviruses that are considered emerging and re-emerging viral pathogens of pigs that pose a significant economic threat to the global pork industry. Although cholesterol is known to affect the replication of a broad range of viruses in vitro, its significance and role in porcine nidovirus infection remains to be elucidated. Therefore, the present study was conducted to determine whether cellular or/and viral cholesterol levels play a role in porcine nidovirus infection...
September 7, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28878794/a-tau-class-glutathione-s-transferase-is-involved-in-trans-resveratrol-transport-out-of-grapevine-cells
#5
Ascensión Martínez-Márquez, María J Martínez-Esteso, María T Vilella-Antón, Susana Sellés-Marchart, Jaime A Morante-Carriel, Elias Hurtado, Javier Palazon, Roque Bru-Martínez
Vitis vinifera cell cultures respond to pathogens and elicitors by synthesizing and extracellularly accumulating stilbenoid phytoalexins. Large amounts of trans-resveratrol (t-R) are produced when a cell culture is elicited with methylated cyclodextrins (MBCD), either alone or combined with methyl jasmonate (MeJA). t-R transport to the extracellular medium, which represents the apoplastic space, would place this antifungal defense right in the battlefield to efficiently fight against pathogen attack. Yet despite their physiological relevance, these transport pathways are mostly unknown...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28878222/angiotensin-ii-induces-cholesterol-accumulation-and-injury-in-podocytes
#6
Yingjie Yang, Qian Yang, Jian Yang, Yiqiong Ma, Guohua Ding
Angiotensin II (Ang II) is a risk factor for the initiation and progression of chronic kidney disease (CKD), as elevated Ang II levels can lead to podocyte injury. However, there have been no studies on the role of Ang II in lipid metabolism or on podocyte injury caused by lipid dysfunction. Our study showed that Ang II induced lipid droplet (LD) accumulation and expression of the LD marker adipose differentiation-related protein (ADRP) in podocytes, and the extent of lipid deposition could be alleviated by losartan...
September 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28863818/depletion-of-membrane-cholesterol-suppresses-drosophila-transient-receptor-potential-like-trpl-channel-activity
#7
Maximilian Peters, Ben Katz, Shaya Lev, Rachel Zaguri, Rita Gutorov, Baruch Minke
Cholesterol is an essential compound of higher eukaryotic cell membranes and a known modulator of ion channel activity. Changes in phospholipids and cholesterol composition of cell membranes are known to alter the activity of ion channels. However, there is little knowledge on the effects of cholesterol on transient receptor potential (TRP) channels. In this study, we explore the effects of cholesterol depletion on the Drosophila photoreceptor channel TRP-like (TRPL), when expressed in tissue culture cells...
2017: Current Topics in Membranes
https://www.readbyqxmd.com/read/28856727/cholesterol-regulates-polymodal-sensory-transduction-in-m%C3%A3-ller-glia
#8
Monika Lakk, Oleg Yarishkin, Jackson M Baumann, Anthony Iuso, David Križaj
Over- and underexposure to cholesterol activates glia in neurodegenerative brain and retinal diseases but the molecular targets of cholesterol in glial cells are not known. Here, we report that disruption of unesterified membrane cholesterol content modulates the transduction of chemical, mechanical and temperature stimuli in mouse Müller cells. Activation of TRPV4 (transient receptor potential vanilloid type 4), a nonselective polymodal cation channel was studied following the removal or supplementation of cholesterol using the methyl-beta cyclodextrin (MβCD) delivery vehicle...
August 30, 2017: Glia
https://www.readbyqxmd.com/read/28831063/sterol-targeting-drugs-reveal-life-cycle-stage-specific-differences-in-trypanosome-lipid-rafts
#9
Aabha I Sharma, Cheryl L Olson, João I Mamede, Felipe Gazos-Lopes, Conrad L Epting, Igor C Almeida, David M Engman
Cilia play important roles in cell signaling, facilitated by the unique lipid environment of a ciliary membrane containing high concentrations of sterol-rich lipid rafts. The African trypanosome Trypanosoma brucei is a single-celled eukaryote with a single cilium/flagellum. We tested whether flagellar sterol enrichment results from selective flagellar partitioning of specific sterol species or from general enrichment of all sterols. While all sterols are enriched in the flagellum, cholesterol is especially enriched...
August 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28822572/enantiodifferentiation-of-whisky-and-cognac-lactones-using-gas-chromatography-with-different-cyclodextrin-chiral-stationary-phases
#10
Hans-Georg Schmarr, Maximilian Mathes, Kristina Wall, Frank Metzner, Marius Fraefel
The chiral lactone 5-butyl-4-methyloxolan-2-one or 5-butyl-4-methyldihydro-2(3H)-furanone, often named whisky lactone, is found in oak wood, then contributing to the appreciated flavor of beverages stored in such wooden barrels. Its next higher homologue is named cognac lactone (5-pentyl-4-methyloxolan-2-one or 5-pentyl-4-methyldihydro-2(3H)-furanone), however is much less known, probably due to its minor concentration level. In order to study the direct enantioseparation of both lactones by gas chromatography on chiral stationary phases, individual enantiomers, particularly for cognac lactone were made available...
August 5, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28821134/graphene-cyclodextrin-based-nanocomposite-hydrogel-with-enhanced-strength-and-thermo-responsive-ability
#11
Pang Zhu, Yonghong Deng, Chaoyang Wang
Dual crosslinked system has been proved to be an efficient method to obtain tough and high strength hydrogels. Herein, we synthesized a novel graphene oxide/p(acrylamide-co-poly(ethylene glycol) methyl ether methacrylate)/α-cyclodextrin (GO/P(AM-co-PEGMA)/CD) physical dual crosslinked hydrogel via copolymerization of AM and PEGMA in the α-CD/GO solution. The polymer main chains adsorb onto the GO surface resulting in the first crosslinked system and multiple hydrogen bonds between α-CDs that thread on the PEGMA side chains establish the second crosslinked system...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28815793/responsiveness-and-morphology-study-of-dual-stimuli-controlled-supramolecular-polymer
#12
Chenxiao Xiong, Meiran Xie, Ruyi Sun
The synthesis and characterization of a linear supramolecular polymer formed by dual host-guest recognitions is presented. The polymer linked by the orthogonal interactions of azobenzene with β-cyclodextrin and methyl viologen with sulfonatocalix[4]arene is constructed, and the morphology change along with the formation and vanishment of host-guest interaction is investigated. The reversible disassembly-reassembly of the polymer induced by light and the redox process are monitored by UV-vis and cyclic voltammetry, respectively...
August 16, 2017: Macromolecular Rapid Communications
https://www.readbyqxmd.com/read/28800107/sequestration-effect-on-the-open-cyclic-switchable-property-of-warfarin-induced-by-cyclodextrin-time-resolved-fluorescence-study
#13
Naji Al-Dubaili, Na'il Saleh
The excited-state lifetimes of the anticoagulant drug warfarin (W) in water and in the absence and presence of methyl-β-cyclodextrins (Me-β-CD) were recorded using time-resolved fluorescence measurements. Selective excitation of the open and cyclic protonated isomers of W were acquired with laser emitting diodes (LED) producing 320 and 280 nm excitation pulses, respectively. Formation of the inclusion complex was checked by UV-visible absorption spectroscopy, and the values of binding constants (2.9 × 10³ M(-1) and 4...
August 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28766240/determination-of-s-lansoprazole-in-dexlansoprazole-preparation-by-capillary-zone-electrophoresis
#14
Hyun Kyu Chung, Quoc-Ky Truong, Xuan-Lan Mai, Yusung Choi, Jong-Seong Kang, Woongchon Mar, Kyeong Ho Kim
Capillary zone electrophoresis was successfully applied to the enantiomeric purity determination of dexlansoprazole using sulfobutyl ether-β-cyclodextrin and methyl-β-cyclodextrin as chiral selectors. Separations were carried out in a 50 μm, 64/56 cm fused-silica capillary. The optimized conditions included 90 mM phosphate buffer, pH 6.0, containing 30 mM sulfobutyl ether-β-cyclodextrin, 20 mM methyl-β-cyclodextrin as background electrolyte, an applied voltage of 25 kV and a temperature of 16 °C, detection was at 280 nm...
August 1, 2017: Archives of Pharmacal Research
https://www.readbyqxmd.com/read/28758845/diloxanide-furoate-binary-complexes-with-%C3%AE-methyl-%C3%AE-and-hydroxypropyl-%C3%AE-cyclodextrins-inclusion-mode-characterization-in-solution-and-in-solid-state-and-in-vitro-dissolution-studies
#15
Carolina Aloisio, Marcela Longhi
The purpose of this study was to investigate the effect on solubility and dissolution rate of binary complexes of β-(βCD), methyl-(MβCD) and hydroxypropyl-β-cyclodextrin (HPβCD) with diloxanide furoate (DF). The complexation in solution was evaluated by phase solubility studies and (1)H nuclear magnetic resonance (NMR). Enhanced water solubility of DF was obtained with the DF:MβCD system (61-fold). The mode of inclusion was supported by NMR experiments, which indicated that real inclusion complexes were formed between DF and MβCD or HPβCD...
August 16, 2017: Pharmaceutical Development and Technology
https://www.readbyqxmd.com/read/28758625/the-dengue-virus-non-structural-protein-1-ns1-is-secreted-from-infected-mosquito-cells-via-a-non-classical-caveolin-1-dependent-pathway
#16
Ana C Alcalá, Raiza Hernández-Bravo, Fernando Medina, David S Coll, Jose L Zambrano, Rosa M Del Angel, Juan E Ludert
Dengue virus NS1 is a glycoprotein of 46-50 kDa that is conserved among flaviviruses, associates as a dimer to cell membranes and is secreted as a hexamer to the extracellular milieu. Recent evidence showed that NS1 is secreted efficiently from infected mosquito cells. To explore the secretory route of NS1 in mosquito cells, infected cells were treated with brefeldin A (BFA) and methyl-beta-cyclodextrin (MβCD). The results showed that MβCD, but not BFA, significantly reduced the release of NS1. Moreover, silencing the expression of caveolin-1 (CAV1; a key component of the caveolar system that transports cholesterol inside the cell), but not SAR1 (a GTPase that participates in the classical secretory pathway), also results in a significant reduction of the secretion of NS1...
August 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28753519/enantioselectivity-of-anteiso-fatty-acids-in-hitherto-uninspected-sample-matrices
#17
Dorothee Eibler, Carolin Seyfried, Walter Vetter
Anteiso-fatty acids (aFAs) are chiral molecules due to a methyl substituent on the antepenultimate carbon of the otherwise straight acyl chain. 12-Methyltetradecanoic acid (a15:0) and 14-methylhexadecanoic acid (a17:0) are the predominant aFAs in nature but their individual contributions e.g. to food lipids are usually low. Enantioselective data has been collected in fish, bovine milk/cheese, and Brussels sprouts. In this study, we determined the enantioselectivity of a15:0 and a17:0 in shea butter, moose and camel milk, two soil samples and mold (collected from contaminated cheese)...
July 20, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28748368/development-and-evaluation-of-ph-responsive-cyclodextrin-based-in-situ-gel-of-paliperidone-for-intranasal-delivery
#18
Atul P Sherje, Vaishali Londhe
Paliperidone (PLPD) is approved for treatment and management of schizophrenia. The current study demonstrates the potential of in situ gel of PLPD for nasal delivery. The permeation of drug through sheep nasal mucosa was analyzed since the nose-to-brain pathway has been indicated for delivering drugs to the brain. The carbopol 934 (CP)- and hydroxypropyl methyl cellulose K4M (HPMC)-based in situ gels containing 0.2% CP and 0.4% w/v HPMC were optimized using experimental design software. The use of hydroxypropyl-β-cyclodextrin (HP-β-CD) in nasal permeation of drug was investigated...
July 26, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28747438/palmitoylation-is-a-prerequisite-for-dimerization-dependent-raftophilicity-of-rhodopsin
#19
Keiji Seno, Fumio Hayashi
The visual photopigment rhodopsin (Rh) is a prototypical G protein-coupled receptor (GPCR) responsible for initiation of the phototransduction cascade in rod photoreceptors. Similar to other GPCRs, Rh can form dimers or even higher oligomers, and tends to have a supramolecular organization that is likely important in the dim light response. Rh also exhibits high affinity for lipid rafts (raftophilicity) upon light-dependent binding with cognate G protein transducin (Gt), suggesting the presence of lipid raft-like domains in the retinal disk membrane and their importance in phototransduction...
July 26, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28729092/annexin-a2-modulated-proliferation-of-pulmonary-arterial-smooth-muscle-cells-depends-on-caveolae-and-caveolin-1-in-hepatopulmonary-syndrome
#20
Lin Liao, Binwu Zheng, Bin Yi, Chang Liu, Lin Chen, Ziyang Zeng, Jing Gao
We have established that annexin A2 (ANXA2) is an important factor in the experimental hepatopulmonary syndrome (HPS) serum-induced proliferation of pulmonary arterial smooth muscle cells (PASMCs). However, the detailed mechanism remains unclear. ANXA2 translocated to the caveolin-enriched microdomains (caveolae) in PASMCs upon HPS serum stimulation. The disruption of caveolae by Methyl-β-cyclodextrin (MβCD) alleviated the caveolae recruitment of ANXA2 and the ANXA2-mediated activation of ERK1/2 and NF-κB, so that ANXA2-modulated PASMC proliferation was suppressed...
July 17, 2017: Experimental Cell Research
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