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Methyl cyclodextrin

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https://www.readbyqxmd.com/read/28214727/a-new-strategy-to-enhance-the-biosynthesis-of-trans-resveratrol-by-overexpressing-stilbene-synthase-gene-in-elicited-vitis-vinifera-cell-cultures
#1
Mingyu Chu, M A Pedreño, Nuria Alburquerque, Lydia Faize, Lorenzo Burgos, Lorena Almagro
In this work, transgenic lines of suspension cultured cells of Vitis vinifera cv. Monastrell containing the plasmid pMOG800-sts have been obtained. The cell growth of these transgenic cell lines decreased slightly as compared to non-transgenic suspension cultured cells, while cell viability was not affected. In addition, the elicitation with cyclodextrins and methyl jasmonate enhanced the production of trans-resveratrol, observing the highest levels of this compound in sts-expressing transgenic Vitis suspension cultured cells with the sts expression cassette in the forwards orientation...
February 8, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28213944/separation-of-hydroxynorketamine-stereoisomers-using-capillary-electrophoresis-with-sulfated-%C3%AE-cyclodextrin-and-highly-sulfated-%C3%AE-cyclodextrin
#2
Friederike A Sandbaumhüter, Regula Theurillat, Wolfgang Thormann
The racemic N-methyl-d-aspartate receptor antagonist ketamine is used in anesthesia, analgesia and the treatment of depressive disorders. It is known that interactions of hydroxylated norketamine metabolites and 5,6-dehydronorketamine (DHNK) with the α7 -nicotinic acetylcholine receptor and the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor are responsible for the antidepressive effects. Ketamine and its first metabolite norketamine are not active on these receptors. As stereoselectivity plays a role in ketamine metabolism, a cationic capillary electrophoresis based method capable of resolving and analyzing the stereoisomers of four hydroxylated norketamine metabolites, norketamine and DHNK was developed...
February 18, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28205316/novel-polymeric-monolith-materials-with-a-%C3%AE-cyclodextrin-graphene-composite-for-the-highly-selective-extraction-of-methyl-jasmonate
#3
Xinhong Yu, Xu Ling, Li Zou, Zilin Chen
A novel polymeric monolith column with a β-cyclodextrins-graphene composite was prepared for extraction of methyl jasmonate. A simple, sensitive and effective polymeric monolith microextraction with high-performance liquid chromatography method has been presented for the determination. To carry out the best microextraction efficiency, several parameters such as sample flow rate, sample volume and sample pH value were systematically optimized. In addition, the method validation showed a wide linear range of 5-2000 ng mL(-) ¹, with a good linearity and the low limits of detection for methyl jasmonate...
February 16, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28204818/reactive-oxygen-species-mediate-angiotensin-ii-induced-transcytosis-of-low-density-lipoprotein-across-endothelial-cells
#4
Fang Bian, Jun Cui, Tao Zheng, Si Jin
The retention of plasma low-density lipoprotein (LDL) particles to subendothelial spaces through transcytosis across the endothelium is the initial step of atherosclerosis (AS). Angiotensin II (Ang II), as the principal effector molecule of the renin-angiotensin system (RAS), is implicated in several important steps of AS development. However, whether or not Ang II can directly exert a pro‑atherogenic effect by promoting LDL transcytosis across endothelial barriers, has not been defined. In the present study, we found that Ang II upregulated intracellular reactive oxygen species (ROS) levels in endothelial cells (ECs) by measuring fluorescence of 2',7'-dichlorofluorescein (DCF‑DA)...
March 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28203712/fertility-of-cold-stored-mouse-sperm-is-recovered-by-promoting-acrosome-reaction-and-hyperactivation-after-cholesterol-efflux-by-methyl-beta-cyclodextrin
#5
Hidetaka Yoshimoto, Toru Takeo, Tetsumi Irie, Naomi Nakagata
No abstract text is available yet for this article.
February 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28194311/tracking-the-virus-like-particles-of-macrobrachium-rosenbergii-nodavirus-in-insect-cells
#6
Ummi Fairuz Hanapi, Chean Yeah Yong, Zee Hong Goh, Noorjahan Banu Alitheen, Swee Keong Yeap, Wen Siang Tan
Macrobrachium rosenbergii nodavirus (MrNv) poses a major threat to the prawn industry. Currently, no effective vaccine and treatment are available to prevent the spread of MrNv. Its infection mechanism and localisation in a host cell are also not well characterised. The MrNv capsid protein (MrNvc) produced in Escherichia coli self-assembled into virus-like particles (VLPs) resembling the native virus. Thus, fluorescein labelled MrNvc VLPs were employed as a model to study the virus entry and localisation in Spodoptera frugiperda, Sf9 cells...
2017: PeerJ
https://www.readbyqxmd.com/read/28185959/improving-the-dissolution-properties-of-curcumin-using-dense-gas-antisolvent-technology
#7
Firman Kurniawansyah, Lisa Quachie, Raffaella Mammucari, Neil R Foster
The dissolution properties of curcumin are notoriously poor and hinder its bioavailability. To improve its dissolution properties, curcumin has been formulated with methyl-β-cyclodextrin and polyvinylpyrrolidone by the atomized rapid injection solvent extraction (ARISE) system. The compounds were co-precipitated from organic solutions using carbon dioxide at 30°C and 95bar as the antisolvent. Curcumin formulations were also produced by physical mixing and freeze drying for comparative purposes. The morphology, crystallinity, solid state molecular interactions, apparent solubility and dissolution profiles of samples were observed...
February 6, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28184226/clathrin-in-chara-australis-molecular-analysis-and-involvement-in-charasome-degradation-and-constitutive-endocytosis
#8
Marion C Hoepflinger, Margit Hoeftberger, Aniela Sommer, Christina Hametner, Ilse Foissner
Charasomes are convoluted plasma membrane domains in characean green algae. They are known to form in response to light via secretion of trans-Golgi network (TGN) vesicles and local inhibition of endocytosis. Charasomes are involved in the acidification of their aqueous environment, thereby facilitating photosynthesis-dependent carbon uptake. Charasome formation is reversible to allow cells to adapt to different light conditions. Here, we show that darkness-induced degradation of charasomes involves the formation of coated pits and coated vesicles...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28183619/self-healing-ph-sensitive-poly-methyl-vinyl-ether-alt-maleic-acid-based-supramolecular-hydrogels-formed-by-inclusion-complexation-between-cyclodextrin-and-adamantane
#9
Xiaoe Ma, Naizhen Zhou, Tianzhu Zhang, Wanjun Hu, Ning Gu
Self-healing materials are of interest for drug delivery, cell and gene therapy, tissue engineering, and other biomedical applications. In this work, on the base of biocompatible polymer poly(methyl vinyl ether-alt-maleic acid) (P(MVE-alt-MA)), host polymer β-cyclodextrin-grafted P(MVE-alt-MA) (P(MVE-alt-MA)-g-β-CD) and guest polymer adamantane-grafted P(MVE-alt-MA) (P(MVE-alt-MA)-g-Ad) were first prepared. Then through taking advantage of the traditional host-guest interaction of β-cyclodextrin and adamantane, a novel self-healing pH-sensitive physical P(MVE-alt-MA)-g-β-CD/P(MVE-alt-MA)-g-Ad supramolecular hydrogels were obtained after simply mixing the aqueous solution of host polymer and guest polymer...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28179028/betanodavirus-like-particles-enter-host-cells-via-clathrin-mediated-endocytosis-in-a-cholesterol-ph-and-cytoskeleton-dependent-manner
#10
Runqing Huang, Guohua Zhu, Jing Zhang, Yuxiong Lai, Yu Xu, Jianguo He, Junfeng Xie
Betanodavirus, also referred to nervous necrosis virus (NNV), is the causative agent of the fatal disease, viral nervous necrosis and has brought significant economic losses in marine and freshwater cultured fish, especially larvae and juveniles. Here, we used an established invasion model with virus-like particle (VLP)-cells, mimicking orange-spotted grouper nervous necrosis virus (OGNNV), to investigate the crucial events of virus entry. VLP were observed in the perinuclear regions of Asian sea bass (SB) cells within 1...
February 8, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28167768/high-resolution-imaging-and-quantification-of-plasma-membrane-cholesterol-by-nanosims
#11
Cuiwen He, Xuchen Hu, Rachel S Jung, Thomas A Weston, Norma P Sandoval, Peter Tontonoz, Matthew R Kilburn, Loren G Fong, Stephen G Young, Haibo Jiang
Cholesterol is a crucial lipid within the plasma membrane of mammalian cells. Recent biochemical studies showed that one pool of cholesterol in the plasma membrane is "accessible" to binding by a modified version of the cytolysin perfringolysin O (PFO*), whereas another pool is sequestered by sphingomyelin and cannot be bound by PFO* unless the sphingomyelin is destroyed with sphingomyelinase (SMase). Thus far, it has been unclear whether PFO* and related cholesterol-binding proteins bind uniformly to the plasma membrane or bind preferentially to specific domains or morphologic features on the plasma membrane...
February 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28167128/testosterone-promotes-tube-formation-of-endothelial-cells-isolated-from-veins-via-activation-of-smad1-protein
#12
Pei Liu, Xiaosa Li, Fuhu Song, Ping Li, Jinzhi Wei, Qing Yan, Xingyan Xu, Jun Yang, Chuanxiang Li, Xiaodong Fu
Testosterone (T) deficiency is positively correlated with the increased incidence of cardiovascular disease. However, the effects of T on vascular endothelial cells remain obscure. Tube formation capacity is critical for vascular regeneration/repair and Smad1 plays an important role in these events. In this study, we investigated the effects of T on Smad1 activation and tube formation of cultured human umbilical endothelial cells (HUVECs). Our results showed that T rapidly increased endothelial Smad1 phosphorylation...
February 5, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28160133/cardioprotective-kinase-signaling-to-subsarcolemmal-and-interfibrillar-mitochondria-is-mediated-by-caveolar-structures
#13
Wylly Ramsés García-Niño, Francisco Correa, Julia Isabel Rodríguez-Barrena, Juan Carlos León-Contreras, Mabel Buelna-Chontal, Elizabeth Soria-Castro, Rogelio Hernández-Pando, José Pedraza-Chaverri, Cecilia Zazueta
The demonstration that caveolin-3 overexpression reduces myocardial ischemia/reperfusion injury and our own finding that multiprotein signaling complexes increase in mitochondria in association with caveolin-3 levels, led us to investigate the contribution of caveolae-driven extracellular signal-regulated kinases 1/2 (ERK1/2) on maintaining the function of cardiac mitochondrial subpopulations from reperfused hearts subjected to postconditioning (PostC). Rat hearts were isolated and subjected to ischemia/reperfusion and to PostC...
March 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/28160078/proteomic-analysis-of-bmn-cell-lipid-rafts-reveals-roles-in-bombyx-mori-nucleopolyhedrovirus-infection
#14
Xiaolong Hu, Min Zhu, Zi Liang, Dhiraj Kumar, Fei Chen, Liyuan Zhu, Sulan Kuang, Renyu Xue, Guangli Cao, Chengliang Gong
The mechanism of how Bombyx mori nucleopolyhedrovirus (BmNPV) enters cells is unknown. The primary components of membrane lipid rafts are proteins and cholesterol, and membrane lipid rafts are thought to be an active region for host-viral interactions. However, whether they contribute to the entry of BmNPV into silkworm cells remains unclear. In this study, we explored the membrane protein components of lipid rafts from BmN cells with mass spectrometry (MS). Proteins and cholesterol were investigated after establishing infection with BmNPV in BmN cells...
February 3, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/28154312/effects-of-m%C3%AE-cd-on-lipoxygenase-induced-ldl-oxidation
#15
Meiying Ao, Yong Chen
Beta-cyclodextrin (β-CD) has been applied as drug/food carriers or potential drugs for treating some diseases. Most recently, some evidence indicated that methyl-β-cyclodextrin (MβCD) and 2-hydroxypropyl-β-cyclodextrin (2-HPβCD), two major derivatives of β-CD, may inhibit atherogenesis, implying that cyclodextrins also can be potential drugs for treating atherosclerosis. It is well known that modification (e.g. oxidation) of low-density lipoprotein (LDL) is one of the most critical steps of atherogenesis...
2017: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28154260/identification-of-adenovirus-derived-cell-penetrating-peptide
#16
Takamasa Hirai, Yoshiaki Yamagishi, Naoya Koizumi, Miwa Nonaka, Rina Mochida, Kenta Shida, Tetsuya Nomura, Makiko Fujii, Fuminori Sakurai, Hiroyuki Mizuguchi, Yoshiteru Watanabe, Naoki Utoguchi
Cell-penetrating peptides (CPPs) have been highly anticipated as an efficient delivery system due to their ability to cross biological membranes and transport various cargoes into cells. In the present study, we have identified adenovirus-derived CPPs using various capsid-mutant adenovirus (Ad) vectors. First, we examined the endocytosis-inducing ability of these vectors. A fiber-shaft substituted Ad vector, Ad type 5 vector with the fiber shaft domain replaced by that derived from Ad type 35, induced the highest fluorescein isothiocyanate (FITC)-dextran uptake into a human liver cell line, HepG2 cells...
2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28153292/excitation-emission-fluorescence-kinetic-data-obtained-by-fenton-degradation-determination-of-heavy-polycyclic-aromatic-hydrocarbons-by-four-way-parallel-factor-analysis
#17
Maira D Carabajal, Juan A Arancibia, Graciela M Escandar
For the first time, a simple and environmentally friendly third-order/four-way calibration was applied for the simultaneous determination of five heavy-polycyclic aromatic hydrocarbons (PAHs) in interfering environments. The kinetic evolution of the Fenton degradation of benzo[a]pyrene, dibenz[a,h]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene and benz[a]anthracene was followed by recording full excitation-emission fluorescence matrices (EEFMs) of the samples at different reaction times, obtaining third-order EEFM-kinetic (EEFM-K) data...
April 1, 2017: Talanta
https://www.readbyqxmd.com/read/28130905/characterization-of-complexes-between-phenethylamine-enantiomers-and-%C3%AE-cyclodextrin-derivatives-by-capillary-electrophoresis-determination-of-binding-constants-and-complex-mobilities
#18
Joachim Wahl, Takayuki Furuishi, Etsuo Yonemochi, Lorenz Meinel, Ulrike Holzgrabe
To optimize chiral separation conditions and to improve the knowledge of enantioseparation it is important to know the binding constants K between analytes and cyclodextrins and the electrophoretic mobilities of the temporarily formed analyte-cyclodextrin-complexes. K values for complexes between 8 phenethylamine enantiomers, namely ephedrine, pseudoephedrine, methylephedrine and norephedrine, and 4 different β-cyclodextrin derivatives were determined by affinity capillary electrophoresis. The binding constants were calculated from the electrophoretic mobility values of the phenethylamine enantiomers at increasing concentrations of cyclodextrins in running buffer...
January 28, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28129550/atp-binding-cassette-a1-abca1-mediates-microparticle-formation-during-high-density-lipoprotein-hdl-biogenesis
#19
Anouar Hafiane, Jacques Genest
BACKGROUND AND AIMS: Micro-particles (MP) are secreted by various cells. Their biological roles in health and in disease remain unknown. Here we describe formation of MP in the process of ABCA1-dependent cholesterol efflux in different cell types. METHODS: The ATP-binding cassette transporter, subfamily A, member 1 (ABCA1) is the rate-limiting step in the biogenesis of high-density lipoproteins (HDL). We have found that ABCA1 and apoA-I contribute to the formation of MP...
January 17, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28123586/downregulation-of-caveolin-1-increases-the-sensitivity-of-drug-resistant-colorectal-cancer-hct116-cells-to-5-fluorouracil
#20
Zhaoyang Li, Ning Wang, Changxin Huang, Yanhong Bao, Yiqian Jiang, Guiting Zhu
Colorectal cancer is the third most common type of cancer in men and women. Chemotherapy is an important treatment strategy for patients with terminal stage cancer. However, the development of drug resistance hampers the effectiveness of chemotherapy. Therefore, an effective therapeutic approach to target chemoresistance-associated cellular molecules is required. In the present study, drug-resistant human colorectal cancer HCT116 cells were developed by treating HCT116 cells with increasing concentrations of 5-fluorouracil (5-FU)...
January 2017: Oncology Letters
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