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https://www.readbyqxmd.com/read/28934426/enhanced-macrophage-m1-polarization-and-resistance-to-apoptosis-enable-resistance-to-plague
#1
Emilia Pachulec, Rym Ben Abdelwahed Bagga, Lucie Chevallier, Hope O'Donnell, Chloé Guillas, Jean Jaubert, Xavier Montagutelli, Elisabeth Carniel, Christian E Demeure
Background: Susceptibility to infection is in part genetically driven, and C57BL/6 mice resist various pathogens through the proinflammatory response of their M1 macrophages (MPs). However, they are susceptible to plague. It has been reported elsewhere that Mus spretus SEG mice resist plague and develop an immune response characterized by a strong recruitment of MPs. Methods: The responses of C57BL/6 and SEG MPs exposed to Yersinia pestis in vitro were examined...
September 15, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28924496/challenging-a-bioinformatic-tool-s-ability-to-detect-microbial-contaminants-using-in-silico-whole-genome-sequencing-data
#2
Nathan D Olson, Justin M Zook, Jayne B Morrow, Nancy J Lin
High sensitivity methods such as next generation sequencing and polymerase chain reaction (PCR) are adversely impacted by organismal and DNA contaminants. Current methods for detecting contaminants in microbial materials (genomic DNA and cultures) are not sensitive enough and require either a known or culturable contaminant. Whole genome sequencing (WGS) is a promising approach for detecting contaminants due to its sensitivity and lack of need for a priori assumptions about the contaminant. Prior to applying WGS, we must first understand its limitations for detecting contaminants and potential for false positives...
2017: PeerJ
https://www.readbyqxmd.com/read/28904964/plague-a-millenary-infectious-disease-reemerging-in-the-xxi-century
#3
REVIEW
A J Dos Santos Grácio, Maria Amélia A Grácio
Plague, in the Middle Ages known as Black Death, continues to occur at permanent foci in many countries, in Africa, Asia, South America, and even the USA. During the last years outbreaks were reported from at least 3 geographical areas, in all cases after tens of years without reported cases. The recent human plague outbreaks in Libya and Algeria suggest that climatic and other environmental changes in Northern Africa may be favourable for Y. pestis epidemiologic cycle. If so, other Northern Africa countries with plague foci also may be at risk for outbreaks in the near future...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28887007/yersinia-pestis-detection-by-loop-mediated-isothermal-amplification-combined-with-magnetic-bead-capture-of-dna
#4
Na Feng, Yazhou Zhou, Yanxiao Fan, Yujing Bi, Ruifu Yang, Yusen Zhou, Xiaoyi Wang
We developed a loop-mediated isothermal amplification (LAMP) assay for the detection of Y. pestis by targeting the 3a sequence on chromosome. All 11 species of the genus Yersinia were used to evaluate the specificity of LAMP and PCR, demonstrating that the primers had a high level of specificity. The sensitivity of LAMP or PCR was 2.3 or 23CFU for pure culture, whereas 2.3×10(4) or 2.3×10(6)CFU for simulated spleen and lung samples. For simulated liver samples, the sensitivity of LAMP was 2.3×10(6)CFU, but PCR was negative at the level of 2...
August 26, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28886687/-fleaing-the-plague-adaptations-of-yersinia-pestis-to-its-insect-vector-that-lead-to-transmission
#5
B Joseph Hinnebusch, Clayton O Jarrett, David M Bland
Interest in arthropod-borne pathogens focuses primarily on how they cause disease in humans. How they produce a transmissible infection in their arthropod host is just as critical to their life cycle, however. Yersinia pestis adopts a unique life stage in the digestive tract of its flea vector, characterized by rapid formation of a bacterial biofilm that is enveloped in a complex extracellular polymeric substance. Localization and adherence of the biofilm to the flea foregut is essential for transmission. Here, we review the molecular and genetic mechanisms of these processes and present a comparative evaluation and updated model of two related transmission mechanisms...
September 8, 2017: Annual Review of Microbiology
https://www.readbyqxmd.com/read/28883503/differential-impact-of-lipopolysaccharide-defects-caused-by-loss-of-rfah-in-yersinia-pseudotuberculosis-and-yersinia-pestis
#6
Jared M Hoffman, Shea Sullivan, Erin Wu, Eric Wilson, David L Erickson
RfaH enhances transcription of a select group of operons controlling bacterial surface features such as lipopolysaccharide (LPS). Previous studies have suggested that rfaH may be required for Yersinia pseudotuberculosis resistance to antimicrobial chemokines and survival during mouse infections. In order to further investigate the role of RfaH in LPS synthesis, resistance to host defense peptides, and virulence of Yersinia, we constructed ΔrfaH mutants of Y. pseudotuberculosis IP32953 and Y. pestis KIM6+. Loss of rfaH affected LPS synthesis in both species, resulting in a shorter core oligosaccharide...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28879613/vaccines-for-conservation-plague-prairie-dogs-black-footed-ferrets-as-a-case-study
#7
Daniel J Salkeld
The endangered black-footed ferret (Mustela nigripes) is affected by plague, caused by Yersinia pestis, both directly, as a cause of mortality, and indirectly, because of the impacts of plague on its prairie dog (Cynomys spp.) prey base. Recent developments in vaccines and vaccine delivery have raised the possibility of plague control in prairie dog populations, thereby protecting ferret populations. A large-scale experimental investigation across the western US shows that sylvatic plague vaccine delivered in oral baits can increase prairie dog survival...
September 6, 2017: EcoHealth
https://www.readbyqxmd.com/read/28873412/temporal-phylogeography-of-yersinia-pestis-in-madagascar-insights-into-the-long-term-maintenance-of-plague
#8
Amy J Vogler, Voahangy Andrianaivoarimanana, Sandra Telfer, Carina M Hall, Jason W Sahl, Crystal M Hepp, Heather Centner, Genevieve Andersen, Dawn N Birdsell, Lila Rahalison, Roxanne Nottingham, Paul Keim, David M Wagner, Minoarisoa Rajerison
BACKGROUND: Yersinia pestis appears to be maintained in multiple, geographically separate, and phylogenetically distinct subpopulations within the highlands of Madagascar. However, the dynamics of these locally differentiated subpopulations through time are mostly unknown. To address that gap and further inform our understanding of plague epidemiology, we investigated the phylogeography of Y. pestis in Madagascar over an 18 year period. METHODOLOGY/PRINCIPAL FINDINGS: We generated whole genome sequences for 31 strains and discovered new SNPs that we used in conjunction with previously identified SNPs and variable-number tandem repeats (VNTRs) to genotype 773 Malagasy Y...
September 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28854255/protein-abundances-can-distinguish-between-naturally-occurring-and-laboratory-strains-of-yersinia-pestis-the-causative-agent-of-plague
#9
Eric D Merkley, Landon H Sego, Andy Lin, Owen P Leiser, Brooke L Deatherage Kaiser, Joshua N Adkins, Paul S Keim, David M Wagner, Helen W Kreuzer
The rapid pace of bacterial evolution enables organisms to adapt to the laboratory environment with repeated passage and thus diverge from naturally-occurring environmental ("wild") strains. Distinguishing wild and laboratory strains is clearly important for biodefense and bioforensics; however, DNA sequence data alone has thus far not provided a clear signature, perhaps due to lack of understanding of how diverse genome changes lead to convergent phenotypes, difficulty in detecting certain types of mutations, or perhaps because some adaptive modifications are epigenetic...
2017: PloS One
https://www.readbyqxmd.com/read/28839028/eight-whole-genome-assemblies-of-yersinia-pestis-subsp-microtus-bv-caucasica-isolated-from-the-common-vole-microtus-arvalis-plague-focus-in-dagestan-russia
#10
Angelina A Kislichkina, Aleksandr G Bogun, Lidiya A Kadnikova, Nadezhda V Maiskaya, Viktor I Solomentsev, Mikhail E Platonov, Svetlana V Dentovskaya, Andrey P Anisimov
We here report the draft genome sequences of 8 Yersinia pestis subsp. microtus bv. caucasica strains isolated from the East Caucasian (previous name, Dagestan) mountain focus (no. 39), representing the most ancient branch of the 0.PE2 phylogroup circulating in populations of common voles (Microtus arvalis).
August 24, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28809473/aggregation-induced-emission-materials-with-different-electric-charges-as-an-artificial-tongue-design-construction-and-assembly-with-various-pathogenic-bacteria-for-effective-bacterial-imaging-and-discrimination
#11
Guang-Jian Liu, Sheng-Nan Tian, Cui-Yun Li, Guo-Wen Xing, Lei Zhou
Imaging-based total bacterial count and type identification of bacteria play crucial roles in clinical diagnostics, public health, biological and medical science, and environmental protection. Herein, we designed and synthesized a series of tetraphenylethenes (TPEs) functionalized with one or two aldehyde, carboxylic acid, and quaternary ammonium groups, which were successfully used as fluorescent materials for rapid and efficient staining of eight kinds of representative bacterial species, including pathogenic bacteria Vibrio cholera, Klebsiella pneumoniae, and Listeria monocytogenes and potential bioterrorism agent Yersinia pestis...
August 30, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28792853/small-scale-die-offs-in-woodrats-support-long-term-maintenance-of-plague-in-the-u-s-southwest
#12
Michael Kosoy, Pamela Reynolds, Ying Bai, Kelly Sheff, Russell E Enscore, John Montenieri, Paul Ettestad, Kenneth Gage
Our longitudinal study of plague dynamics was conducted in north-central New Mexico to identify which species in the community were infected with plague, to determine the spatial and temporal patterns of the dynamics of plague epizootics, and to describe the dynamics of Yersinia pestis infection within individual hosts. A total of 3156 fleas collected from 535 small mammals of 8 species were tested for Y. pestis DNA. Nine fleas collected from six southern plains woodrats (Neotoma micropus) and from one rock squirrel (Otospermophilus variegatus) were positive for the pla gene of Y...
September 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28754138/serological-and-pcr-investigation-of-yersinia-pestis-in-potential-reservoir-hosts-from-a-plague-outbreak-focus-in-zambia
#13
S S Nyirenda, B M Hang'ombe, E Mulenga, B S Kilonzo
BACKGROUND: Plague is a bacterial zoonotic disease, caused by Yersinia pestis. Rodents are the natural hosts with fleas as the vehicle of disease transmission. Domestic and wild dogs and cats have also been identified as possible disease hosts. In Zambia, plague outbreaks have been reported in the Southern and Eastern regions in the last 20 years. Based on these observations, Y. pestis could possibly be endemically present in the area. METHODS: To substantiate such possibility, sera samples were collected from rodents, shrews, dogs and cats for detection of antibodies against Fraction 1 gene (Fra1) of Y...
July 28, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28750048/competition-is-the-basis-of-the-transport-mechanism-of-the-nhab-na-h-exchanger-from-klebsiella-pneumoniae
#14
Miyer Patiño-Ruiz, Constanța Ganea, Klaus Fendler, Octavian Călinescu
Na+/H+ exchange is essential for survival of all organisms, having a role in the regulation of the intracellular Na+ concentration, pH and cell volume. Furthermore, Na+/H+ exchangers were shown to be involved in the virulence of the bacterium Yersinia pestis, indicating they might be potential targets for novel antibiotic treatments. The model system for Na+/H+ exchangers is the NhaA transporter from Escherichia coli, EcNhaA. Therefore, the general transport mechanism of NhaA exchangers is currently well characterized...
2017: PloS One
https://www.readbyqxmd.com/read/28746380/paleoproteomics-of-the-dental-pulp-the-plague-paradigm
#15
Rémi Barbieri, Rania Mekni, Anthony Levasseur, Eric Chabrière, Michel Signoli, Stéfan Tzortzis, Gérard Aboudharam, Michel Drancourt
Chemical decomposition and fragmentation may limit the detection of ancient host and microbial DNA while some proteins can be detected for extended periods of time. We applied paleoproteomics on 300-year-old dental pulp specimens recovered from 16 individuals in two archeological funeral sites in France, comprising one documented plague site and one documented plague-negative site. The dental pulp paleoproteome of the 16 teeth comprised 439 peptides representative of 30 proteins of human origin and 211 peptides representative of 27 proteins of non-human origin...
2017: PloS One
https://www.readbyqxmd.com/read/28743813/curative-treatment-of-severe-gram-negative-bacterial-infections-by-a-new-class-of-antibiotics-targeting-lpxc
#16
Nadine Lemaître, Xiaofei Liang, Javaria Najeeb, Chul-Jin Lee, Marie Titecat, Emmanuelle Leteurtre, Michel Simonet, Eric J Toone, Pei Zhou, Florent Sebbane
The infectious diseases caused by multidrug-resistant bacteria pose serious threats to humankind. It has been suggested that an antibiotic targeting LpxC of the lipid A biosynthetic pathway in Gram-negative bacteria is a promising strategy for curing Gram-negative bacterial infections. However, experimental proof of this concept is lacking. Here, we describe our discovery and characterization of a biphenylacetylene-based inhibitor of LpxC, an essential enzyme in the biosynthesis of the lipid A component of the outer membrane of Gram-negative bacteria...
July 25, 2017: MBio
https://www.readbyqxmd.com/read/28737374/integrated-sers-based-microdroplet-platform-for-the-automated-immunoassay-of-f1-antigens-in-yersinia-pestis
#17
Namhyun Choi, Jiyoung Lee, Juhui Ko, Jun Ho Jeon, Gi-Eun Rhie, Andrew J deMello, Jaebum Choo
The development of surface-enhanced Raman scattering (SERS)-based microfluidic platforms has attracted significant recent attention in the biological sciences. SERS is a highly sensitive detection modality, with microfluidic platforms providing many advantages over microscale methods, including high analytical throughput, facile automation, and reduced sample requirements. Accordingly, the integration of SERS with microfluidic platforms offers significant utility in chemical and biological experimentation. Herein, we report a fully integrated SERS-based microdroplet platform for the automatic immunoassay of specific antigen fraction 1 (F1) in Yersinia pestis...
August 3, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28732479/an-integrated-computational-experimental-approach-reveals-yersinia-pestis-genes-essential-across-a-narrow-or-a-broad-range-of-environmental-conditions
#18
Nicola J Senior, Kalesh Sasidharan, Richard J Saint, Andrew E Scott, Mitali Sarkar-Tyson, Philip M Ireland, Helen L Bullifent, Z Rong Yang, Karen Moore, Petra C F Oyston, Timothy P Atkins, Helen S Atkins, Orkun S Soyer, Richard W Titball
BACKGROUND: The World Health Organization has categorized plague as a re-emerging disease and the potential for Yersinia pestis to also be used as a bioweapon makes the identification of new drug targets against this pathogen a priority. Environmental temperature is a key signal which regulates virulence of the bacterium. The bacterium normally grows outside the human host at 28 °C. Therefore, understanding the mechanisms that the bacterium used to adapt to a mammalian host at 37 °C is central to the development of vaccines or drugs for the prevention or treatment of human disease...
July 21, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28726410/structural-insights-into-the-yersinia-pestis-outer-membrane-protein-ail-in-lipid-bilayers
#19
Samit Kumar Dutta, Yong Yao, Francesca M Marassi
Yersinia pestis the causative agent of plague, is highly pathogenic and poses very high risk to public health. The outer membrane protein Ail (Adhesion invasion locus) is one of the most highly expressed proteins on the cell surface of Y. pestis, and a major target for the development of medical countermeasures. Ail is essential for microbial virulence and is critical for promoting the survival of Y. pestis in serum. Structures of Ail have been determined by X-ray diffraction and solution NMR spectroscopy, but the protein's activity is influenced by the detergents in these samples, underscoring the importance of the surrounding environment for structure-activity studies...
August 4, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28722614/identification-of-risk-factors-associated-with-transmission-of-plague-disease-in-eastern-zambia
#20
Stanley S Nyirenda, Bernard M Hang'ombe, Robert Machangu, Jackson Mwanza, Bukheti S Kilonzo
Plague is a fatal, primarily rodent-flea-borne zoonotic disease caused by Yersinia pestis. The identification of risk factors of plague was investigated through questionnaire interview and conducting focus group discussion (FGD) in Sinda and Nyimba of eastern Zambia. A total of 104 questionnaires were administered to individual respondents and 20 groups consisting of 181 discussants, which comprised FGD team in this study. The study revealed that trapping, transportation, and preparation of rodents for food exposed the community to rodent and their fleas suggesting that plague may have occurred primarily by either flea bites or contact with infected wild rodents...
June 12, 2017: American Journal of Tropical Medicine and Hygiene
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