keyword
MENU ▼
Read by QxMD icon Read
search

pestis

keyword
https://www.readbyqxmd.com/read/28081130/comparative-ability-of-oropsylla-montana-and-xenopsylla-cheopis-fleas-to-transmit-yersinia-pestis-by-two-different-mechanisms
#1
B Joseph Hinnebusch, David M Bland, Christopher F Bosio, Clayton O Jarrett
BACKGROUND: Transmission of Yersinia pestis by flea bite can occur by two mechanisms. After taking a blood meal from a bacteremic mammal, fleas have the potential to transmit the very next time they feed. This early-phase transmission resembles mechanical transmission in some respects, but the mechanism is unknown. Thereafter, transmission occurs after Yersinia pestis forms a biofilm in the proventricular valve in the flea foregut. The biofilm can impede and sometimes completely block the ingestion of blood, resulting in regurgitative transmission of bacteria into the bite site...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28068343/the-fleas-siphonaptera-in-iran-diversity-host-range-and-medical-importance
#2
Naseh Maleki-Ravasan, Samaneh Solhjouy-Fard, Jean-Claude Beaucournu, Anne Laudisoit, Ehsan Mostafavi
BACKGROUND: Flea-borne diseases have a wide distribution in the world. Studies on the identity, abundance, distribution and seasonality of the potential vectors of pathogenic agents (e.g. Yersinia pestis, Francisella tularensis, and Rickettsia felis) are necessary tools for controlling and preventing such diseases outbreaks. The improvements of diagnostic tools are partly responsible for an easier detection of otherwise unnoticed agents in the ectoparasitic fauna and as such a good taxonomical knowledge of the potential vectors is crucial...
January 9, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28067495/upconversion-nanocrystals-mediated-lateral-flow-nanoplatform-for-in-vitro-detection
#3
Zhiqin Liang, Xiaochen Wang, Wei Zhu, Pingping Zhang, Yongxin Yang, Chongyun Sun, Junjie Zhang, Xinrui Wang, Zheng Xu, Yong Zhao, Ruifu Yang, Suling Zhao, Lei Zhou
Upconversion phosphors (UCPs) that are free from interference from biological sample autofluorescence have attracted attention for in vivo and in vitro bio-applications. However, UCPs need to be water-dispersible, nanosized, and highly luminous to realize broad applications. Therefore, the aim of this research is to develop UCPs that meet these comprehensive criteria for in vitro diagnosis. To combine nanosize with high luminous intensity, β-NaYF4:Yb3+,Er3+ upconversion nanocrystals (UCNPs) codoped with Li+ and K+ are prepared that display high upconversion intensities as well as small size...
January 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28054667/yersinia-pestis-halotolerance-illuminates-plague-reservoirs
#4
Maliya Alia Malek, Idir Bitam, Anthony Levasseur, Jérôme Terras, Jean Gaudart, Said Azza, Christophe Flaudrops, Catherine Robert, Didier Raoult, Michel Drancourt
The plague agent Yersinia pestis persists for years in the soil. Two millennia after swiping over Europe and North Africa, plague established permanent foci in North Africa but not in neighboring Europe. Mapping human plague foci reported in North Africa for 70 years indicated a significant location at <3 kilometers from the Mediterranean seashore or the edge of salted lakes named chotts. In Algeria, culturing 352 environmental specimens naturally containing 0.5 to 70 g/L NaCl yielded one Y. pestis Orientalis biotype isolate in a 40 g/L NaCl chott soil specimen...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28043017/evolution-and-structural-dynamics-of-bacterial-glycan-binding-adhesins
#5
REVIEW
Kristof Moonens, Han Remaut
Infectious disease processes like bacterial adherence or the activity of secreted toxins frequently gain host and tissue specificity by glycan binding interactions with the host glycome. Recent functional and structural studies highlight the high niche specialization of bacterial lectins, but also reveal a remarkable plasticity in their glycan binding sites and mechanisms, to adapt to host glycome dynamics or changing environmental conditions at the site of infection. In this review we put emphasis on new structural insights in host adaptation and dynamics of bacterial carbohydrate binding adhesins and toxins in human pathogens like uropathogenic and enteropathogenic Escherichia coli, Helicobacter pylori, Yersinia pestis or Vibrio cholera...
December 30, 2016: Current Opinion in Structural Biology
https://www.readbyqxmd.com/read/28030576/functional-and-structural-analysis-of-a-highly-expressed-yersinia-pestis-small-rna-following-infection-of-cultured-macrophages
#6
Nan Li, Scott P Hennelly, Chris J Stubben, Sofiya Micheva-Viteva, Bin Hu, Yulin Shou, Momchilo Vuyisich, Chang-Shung Tung, Patrick S Chain, Karissa Y Sanbonmatsu, Elizabeth Hong-Geller
Non-coding small RNAs (sRNAs) are found in practically all bacterial genomes and play important roles in regulating gene expression to impact bacterial metabolism, growth, and virulence. We performed transcriptomics analysis to identify sRNAs that are differentially expressed in Yersinia pestis that invaded the human macrophage cell line THP-1, compared to pathogens that remained extracellular in the presence of host. Using ultra high-throughput sequencing, we identified 37 novel and 143 previously known sRNAs in Y...
2016: PloS One
https://www.readbyqxmd.com/read/28019002/characterization-of-yersinia-species-by-protein-profiling-using-automated-microfluidic-capillary-electrophoresis
#7
Victoria M Bennett, Dennis McNevin, Paul Roffey, Michelle E Gahan
Yersinia pestis is a biological agent of high risk to national security due to its ability to be easily disseminated and transmitted among humans. If Y. pestis was to be utilized in a deliberate disease outbreak it would be essential to rapidly and accurately identify the agent. Current identification methods for Yersinia species are limited by their reliance on cultivation, the time taken to achieve results and/or the use of protocols that are not amenable for field use. Faster identification methods are urgently required...
December 26, 2016: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/28018363/plague-vaccine-development-current-research-and-future-trends
#8
REVIEW
Shailendra Kumar Verma, Urmil Tuteja
Plague is one of the world's most lethal human diseases caused by Yersinia pestis, a Gram-negative bacterium. Despite overwhelming studies for many years worldwide, there is no safe and effective vaccine against this fatal disease. Inhalation of Y. pestis bacilli causes pneumonic plague, a fast growing and deadly dangerous disease. F1/LcrV-based vaccines failed to provide adequate protection in African green monkey model in spite of providing protection in mice and cynomolgus macaques. There is still no explanation for this inconsistent efficacy, and scientists leg behind to search reliable correlate assays for immune protection...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27981238/new-insights-into-autoinducer-2-signaling-as-a-virulence-regulator-in-a-mouse-model-of-pneumonic-plague
#9
Eric C Fitts, Jourdan A Andersson, Michelle L Kirtley, Jian Sha, Tatiana E Erova, Sadhana Chauhan, Vladimir L Motin, Ashok K Chopra
The Enterobacteriaceae family members, including the infamous Yersinia pestis, the causative agent of plague, have a highly conserved interbacterial signaling system that is mediated by the autoinducer-2 (AI-2) quorum-sensing molecule. The AI-2 system is implicated in regulating various bacterial virulence genes in diverse environmental niches. Deletion of the gene encoding the synthetic enzyme for the AI-2 substrate, luxS, leads to either no significant change or, paradoxically, an increase in in vivo bacterial virulence...
November 2016: MSphere
https://www.readbyqxmd.com/read/27936190/two-isoforms-of-yersinia-pestis-plasminogen-activator-pla-intraspecies-distribution-intrinsic-disorder-propensity-and-contribution-to-virulence
#10
Svetlana V Dentovskaya, Mikhail E Platonov, Tat'yana E Svetoch, Pavel Kh Kopylov, Tat'yana I Kombarova, Sergey A Ivanov, Rima Z Shaikhutdinova, Lyubov' V Kolombet, Sadhana Chauhan, Vitaly G Ablamunits, Vladimir L Motin, Vladimir N Uversky, Andrey P Anisimov
It has been shown previously that several endemic Y. pestis isolates with limited virulence contained the I259 isoform of the outer membrane protease Pla, while the epidemic highly virulent strains possessed only the T259 Pla isoform. Our sequence analysis of the pla gene from 118 Y. pestis subsp. microtus strains revealed that the I259 isoform was present exclusively in the endemic strains providing a convictive evidence of more ancestral origin of this isoform. Analysis of the effects of the I259T polymorphism on the intrinsic disorder propensity of Pla revealed that the I259T mutation slightly increases the intrinsic disorder propensity of the C-terminal tail of Pla and makes this protein slightly more prone for disorder-based protein-protein interactions, suggesting that the T259 Pla could be functionally more active than the I259 Pla...
2016: PloS One
https://www.readbyqxmd.com/read/27929533/the-yersinia-type-iii-secretion-effector-yopm-is-an-e3-ubiquitin-ligase-that-induced-necrotic-cell-death-by-targeting-nlrp3
#11
Congwen Wei, Ying Wang, Zongmin Du, Kai Guan, Ye Cao, Huiying Yang, Pengyu Zhou, Feixiang Wu, Jiankang Chen, Penghao Wang, Zirui Zheng, Pingping Zhang, Yanhong Zhang, Shengli Ma, Ruifu Yang, Hui Zhong, Xiang He
Yersinia pestis uses type III effector proteins to target eukaryotic signaling systems. The Yersinia outer protein (Yop) M effector from the Y. pestis strain is a critical virulence determinant; however, its role in Y. pestis pathogenesis is just beginning to emerge. Here we first identify YopM as the structural mimic of the bacterial IpaH E3 ligase family in vitro, and establish that the conserved CLD motif in its N-terminal is responsible for the E3 ligase function. Furthermore, we show that NLRP3 is a novel target of the YopM protein...
December 8, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27911947/the-yersinia-pestis-effector-yopm-inhibits-pyrin-inflammasome-activation
#12
Dmitry Ratner, M Pontus A Orning, Megan K Proulx, Donghai Wang, Mikhail A Gavrilin, Mark D Wewers, Emad S Alnemri, Peter F Johnson, Bettina Lee, Joan Mecsas, Nobuhiko Kayagaki, Jon D Goguen, Egil Lien
Type III secretion systems (T3SS) are central virulence factors for many pathogenic Gram-negative bacteria, and secreted T3SS effectors can block key aspects of host cell signaling. To counter this, innate immune responses can also sense some T3SS components to initiate anti-bacterial mechanisms. The Yersinia pestis T3SS is particularly effective and sophisticated in manipulating the production of pro-inflammatory cytokines IL-1β and IL-18, which are typically processed into their mature forms by active caspase-1 following inflammasome formation...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27889163/differential-detection-of-pathogenic-yersinia-spp-by-fluorescence-in-situ-hybridization
#13
Alexander Rohde, Jens Andre Hammerl, Bernd Appel, Ralf Dieckmann, Sascha Al Dahouk
Yersinia enterocolitica, Y. pseudotuberculosis and Y. pestis are pathogens of major medical importance, which are responsible for a considerable number of infections every year. The detection of these species still relies on cultural methods, which are slow, labour intensive and often hampered by the presence of high amounts of accompanying flora. In this study, fluorescence in situ hybridization (FISH) was used to develop a fast, sensitive and reliable alternative to detect viable bacteria in food. For this purpose, highly specific probes targeting the 16S and 23S ribosomal RNA were employed to differentially detect each of the three species...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27876297/host-transcriptomic-responses-to-pneumonic-plague-reveal-that-yersinia-pestis-inhibits-both-the-initial-adaptive-and-innate-immune-responses-in-mice
#14
Huiying Yang, Tong Wang, Guang Tian, Qingwen Zhang, Xiaohong Wu, Youqian Xin, Yanfeng Yan, Yafang Tan, Shiyang Cao, Wanbing Liu, Yujun Cui, Ruifu Yang, Zongmin Du
Pneumonic plague is the most deadly form of infection caused by Yersinia pestis and can progress extremely fast. However, our understanding on the host transcriptomic response to pneumonic plague is insufficient. Here, we used RNA-sequencing technology to analyze transcriptomic responses in mice infected with fully virulent strain 201 or EV76, a live attenuated vaccine strain lacking the pigmentation locus. Approximately 600 differentially expressed genes (DEGs) were detected in lungs from both 201- and EV76-infected mice at 12h post-infection (hpi)...
November 14, 2016: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/27874136/a-self-quenching-resistant-carbon-nanodot-powder-with-multicolored-solid-state-fluorescence-for-ultra-fast-staining-of-various-representative-bacterial-species-within-one-minute
#15
Yongqiang Zhang, Chunfeng Li, Yi Fan, Chengbin Wang, Ruifu Yang, Xingyuan Liu, Lei Zhou
In this study, we prepared self-quenching-resistant solid-state fluorescent carbon nanodots (SFCDs) without any other solid matrices. The SFCDs were prepared using a one-step microwave synthesis method through precise control of the heating power and time. The resulting SFCD powder showed excitation-dependent emission behavior with a maximum fluorescence quantum yield of 40%. The multicolored SFCDs were successfully used as fluorescent agents for rapid staining of 14 representative bacterial species, including Gram-negative, Gram-positive, and acid-fast bacteria...
December 1, 2016: Nanoscale
https://www.readbyqxmd.com/read/27870634/investigation-of-and-response-to-2-plague-cases-yosemite-national-park-california-usa-2015
#16
Mary Danforth, Mark Novak, Jeannine Petersen, Paul Mead, Luke Kingry, Matthew Weinburke, Danielle Buttke, Gregory Hacker, James Tucker, Michael Niemela, Bryan Jackson, Kerry Padgett, Kelly Liebman, Duc Vugia, Vicki Kramer
In August 2015, plague was diagnosed for 2 persons who had visited Yosemite National Park in California, USA. One case was septicemic and the other bubonic. Subsequent environmental investigation identified probable locations of exposure for each patient and evidence of epizootic plague in other areas of the park. Transmission of Yersinia pestis was detected by testing rodent serum, fleas, and rodent carcasses. The environmental investigation and whole-genome multilocus sequence typing of Y. pestis isolates from the patients and environmental samples indicated that the patients had been exposed in different locations and that at least 2 distinct strains of Y...
December 2016: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/27837489/a-method-for-characterizing-the-type-iii-secretion-system-s-contribution-to-pathogenesis-homologous-recombination-to-generate-yersinia-pestis-type-iii-secretion-system-mutants
#17
Patrick Osei-Owusu, Matthew L Nilles, David S Bradley, Travis D Alvine
The type III (T3S) secretion system of many gram-negative bacteria is a surface-exposed protein secretion apparatus used to directly inject bacterial effector molecules into eukaryotic cells. These effector molecules contribute to bacterial pathogenesis in many ways, and have been shown to be crucial for infectivity. Here, we describe a protocol for using homologous recombination to generate T3S system mutants to assess the role of different T3S system proteins in bacterial pathogenesis.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27837486/measurement-of-effector-protein-translocation-using-phosphorylatable-epitope-tags-and-phospho-specific-antibodies
#18
Sara Schesser Bartra, Gregory V Plano
Numerous bacterial pathogens employ specialized protein secretion machineries to directly inject anti-host proteins, termed effector proteins, into eukaryotic cells. Effector proteins carrying small phosphorylatable tags can be used to detect and quantify effector protein injection. Here, we describe the use of the ELK- and GSK-tags to detect the translocation of the Y. pestis YopE effector protein into RAW 264.7 macrophage-like cells using immunoblot analysis with phospho-specific antibodies.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27837480/in-vivo-photo-cross-linking-to-study-t3s-interactions-demonstrated-using-the-yersinia-pestis-t3s-system
#19
Thomas A Henderson, Matthew L Nilles
Cross-linking of proteins is effective in determining protein-protein interactions. The use of photo-cross-linkers was developed to study protein interactions in several manners. One method involved the incorporation of photo-activatable cross-linking groups into chemically synthesized peptides. A second approach relies on incorporation of photo-activatable cross-linking groups into proteins using tRNAs with chemically bound photo-activatable amino acids with suppressor tRNAs translational systems to incorporate the tags into specific sites...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27837479/blue-native-protein-electrophoresis-to-study-the-t3s-system-using-yersinia-pestis-as-a-model
#20
Thomas A Henderson, Matthew L Nilles
Since the introduction of blue native, clear native, and high-resolution clear native electrophoresis to study protein complexes of eukaryotic, bacterial, and archaeal cells, the technique has been used primarily to study physiological systems that are found in abundance within the cell. Systems involved in oxidative phosphorylation, electron transport, membrane transporters, and secretion systems have been studied using these techniques. These microscale techniques are ideal due to the minimal perturbations caused to these protein complexes...
2017: Methods in Molecular Biology
keyword
keyword
85966
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"