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https://www.readbyqxmd.com/read/27929533/the-yersinia-type-iii-secretion-effector-yopm-is-an-e3-ubiquitin-ligase-that-induced-necrotic-cell-death-by-targeting-nlrp3
#1
Congwen Wei, Ying Wang, Zongmin Du, Kai Guan, Ye Cao, Huiying Yang, Pengyu Zhou, Feixiang Wu, Jiankang Chen, Penghao Wang, Zirui Zheng, Pingping Zhang, Yanhong Zhang, Shengli Ma, Ruifu Yang, Hui Zhong, Xiang He
Yersinia pestis uses type III effector proteins to target eukaryotic signaling systems. The Yersinia outer protein (Yop) M effector from the Y. pestis strain is a critical virulence determinant; however, its role in Y. pestis pathogenesis is just beginning to emerge. Here we first identify YopM as the structural mimic of the bacterial IpaH E3 ligase family in vitro, and establish that the conserved CLD motif in its N-terminal is responsible for the E3 ligase function. Furthermore, we show that NLRP3 is a novel target of the YopM protein...
December 8, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27911947/the-yersinia-pestis-effector-yopm-inhibits-pyrin-inflammasome-activation
#2
Dmitry Ratner, M Pontus A Orning, Megan K Proulx, Donghai Wang, Mikhail A Gavrilin, Mark D Wewers, Emad S Alnemri, Peter F Johnson, Bettina Lee, Joan Mecsas, Nobuhiko Kayagaki, Jon D Goguen, Egil Lien
Type III secretion systems (T3SS) are central virulence factors for many pathogenic Gram-negative bacteria, and secreted T3SS effectors can block key aspects of host cell signaling. To counter this, innate immune responses can also sense some T3SS components to initiate anti-bacterial mechanisms. The Yersinia pestis T3SS is particularly effective and sophisticated in manipulating the production of pro-inflammatory cytokines IL-1β and IL-18, which are typically processed into their mature forms by active caspase-1 following inflammasome formation...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27889163/differential-detection-of-pathogenic-yersinia-spp-by-fluorescence-in-situ-hybridization
#3
Alexander Rohde, Jens Andre Hammerl, Bernd Appel, Ralf Dieckmann, Sascha Al Dahouk
Yersinia enterocolitica, Y. pseudotuberculosis and Y. pestis are pathogens of major medical importance, which are responsible for a considerable number of infections every year. The detection of these species still relies on cultural methods, which are slow, labour intensive and often hampered by the presence of high amounts of accompanying flora. In this study, fluorescence in situ hybridization (FISH) was used to develop a fast, sensitive and reliable alternative to detect viable bacteria in food. For this purpose, highly specific probes targeting the 16S and 23S ribosomal RNA were employed to differentially detect each of the three species...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27876297/host-transcriptomic-responses-to-pneumonic-plague-reveal-that-yersinia-pestis-inhibits-both-the-initial-adaptive-and-innate-immune-responses-in-mice
#4
Huiying Yang, Tong Wang, Guang Tian, Qingwen Zhang, Xiaohong Wu, Youqian Xin, Yanfeng Yan, Yafang Tan, Shiyang Cao, Wanbing Liu, Yujun Cui, Ruifu Yang, Zongmin Du
Pneumonic plague is the most deadly form of infection caused by Yersinia pestis and can progress extremely fast. However, our understanding on the host transcriptomic response to pneumonic plague is insufficient. Here, we used RNA-sequencing technology to analyze transcriptomic responses in mice infected with fully virulent strain 201 or EV76, a live attenuated vaccine strain lacking the pigmentation locus. Approximately 600 differentially expressed genes (DEGs) were detected in lungs from both 201- and EV76-infected mice at 12h post-infection (hpi)...
November 14, 2016: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/27874136/a-self-quenching-resistant-carbon-nanodot-powder-with-multicolored-solid-state-fluorescence-for-ultra-fast-staining-of-various-representative-bacterial-species-within-one-minute
#5
Yongqiang Zhang, Chunfeng Li, Yi Fan, Chengbin Wang, Ruifu Yang, Xingyuan Liu, Lei Zhou
In this study, we prepared self-quenching-resistant solid-state fluorescent carbon nanodots (SFCDs) without any other solid matrices. The SFCDs were prepared using a one-step microwave synthesis method through precise control of the heating power and time. The resulting SFCD powder showed excitation-dependent emission behavior with a maximum fluorescence quantum yield of 40%. The multicolored SFCDs were successfully used as fluorescent agents for rapid staining of 14 representative bacterial species, including Gram-negative, Gram-positive, and acid-fast bacteria...
December 1, 2016: Nanoscale
https://www.readbyqxmd.com/read/27870634/investigation-of-and-response-to-2-plague-cases-yosemite-national-park-california-usa-2015
#6
Mary Danforth, Mark Novak, Jeannine Petersen, Paul Mead, Luke Kingry, Matthew Weinburke, Danielle Buttke, Gregory Hacker, James Tucker, Michael Niemela, Bryan Jackson, Kerry Padgett, Kelly Liebman, Duc Vugia, Vicki Kramer
In August 2015, plague was diagnosed for 2 persons who had visited Yosemite National Park in California, USA. One case was septicemic and the other bubonic. Subsequent environmental investigation identified probable locations of exposure for each patient and evidence of epizootic plague in other areas of the park. Transmission of Yersinia pestis was detected by testing rodent serum, fleas, and rodent carcasses. The environmental investigation and whole-genome multilocus sequence typing of Y. pestis isolates from the patients and environmental samples indicated that the patients had been exposed in different locations and that at least 2 distinct strains of Y...
December 2016: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/27837489/a-method-for-characterizing-the-type-iii-secretion-system-s-contribution-to-pathogenesis-homologous-recombination-to-generate-yersinia-pestis-type-iii-secretion-system-mutants
#7
Patrick Osei-Owusu, Matthew L Nilles, David S Bradley, Travis D Alvine
The type III (T3S) secretion system of many gram-negative bacteria is a surface-exposed protein secretion apparatus used to directly inject bacterial effector molecules into eukaryotic cells. These effector molecules contribute to bacterial pathogenesis in many ways, and have been shown to be crucial for infectivity. Here, we describe a protocol for using homologous recombination to generate T3S system mutants to assess the role of different T3S system proteins in bacterial pathogenesis.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27837486/measurement-of-effector-protein-translocation-using-phosphorylatable-epitope-tags-and-phospho-specific-antibodies
#8
Sara Schesser Bartra, Gregory V Plano
Numerous bacterial pathogens employ specialized protein secretion machineries to directly inject anti-host proteins, termed effector proteins, into eukaryotic cells. Effector proteins carrying small phosphorylatable tags can be used to detect and quantify effector protein injection. Here, we describe the use of the ELK- and GSK-tags to detect the translocation of the Y. pestis YopE effector protein into RAW 264.7 macrophage-like cells using immunoblot analysis with phospho-specific antibodies.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27837480/in-vivo-photo-cross-linking-to-study-t3s-interactions-demonstrated-using-the-yersinia-pestis-t3s-system
#9
Thomas A Henderson, Matthew L Nilles
Cross-linking of proteins is effective in determining protein-protein interactions. The use of photo-cross-linkers was developed to study protein interactions in several manners. One method involved the incorporation of photo-activatable cross-linking groups into chemically synthesized peptides. A second approach relies on incorporation of photo-activatable cross-linking groups into proteins using tRNAs with chemically bound photo-activatable amino acids with suppressor tRNAs translational systems to incorporate the tags into specific sites...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27837479/blue-native-protein-electrophoresis-to-study-the-t3s-system-using-yersinia-pestis-as-a-model
#10
Thomas A Henderson, Matthew L Nilles
Since the introduction of blue native, clear native, and high-resolution clear native electrophoresis to study protein complexes of eukaryotic, bacterial, and archaeal cells, the technique has been used primarily to study physiological systems that are found in abundance within the cell. Systems involved in oxidative phosphorylation, electron transport, membrane transporters, and secretion systems have been studied using these techniques. These microscale techniques are ideal due to the minimal perturbations caused to these protein complexes...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27832215/avoiding-pandemic-fears-in-the-subway-and-conquering-the-platypus
#11
COMMENT
A Gonzalez, Y Vázquez-Baeza, J B Pettengill, A Ottesen, D McDonald, R Knight
Metagenomics is increasingly used not just to show patterns of microbial diversity but also as a culture-independent method to detect individual organisms of intense clinical, epidemiological, conservation, forensic, or regulatory interest. A widely reported metagenomic study of the New York subway suggested that the pathogens Yersinia pestis and Bacillus anthracis were part of the "normal subway microbiome." In their article in mSystems, Hsu and collaborators (mSystems 1(3):e00018-16, 2016, http://dx.doi.org/10...
May 2016: MSystems
https://www.readbyqxmd.com/read/27830695/crystal-structure-of-bacterial-haem-importer-complex-in-the-inward-facing-conformation
#12
Youichi Naoe, Nozomi Nakamura, Akihiro Doi, Mia Sawabe, Hiro Nakamura, Yoshitsugu Shiro, Hiroshi Sugimoto
Pathogenic bacteria remove iron from the haem of host tissues and use it as a catalytic center of many enzymes. Haem uptake by pathogenic bacteria is facilitated by the membrane-integrated haem importer, which belongs to the type II ATP-binding cassette (ABC) transporter. Here we present crystal structures of Burkholderia cenocepacia haem importer BhuUV complexed with the periplasmic haem-binding protein BhuT and in the absence of BhuT. The transmembrane helices of these structures show an inward-facing conformation, in which the cytoplasmic gate of the haem translocation pathway is completely open...
November 10, 2016: Nature Communications
https://www.readbyqxmd.com/read/27826675/effects-of-clary-sage-oil-and-its-main-components-linalool-and-linalyl-acetate-on-the-plasma-membrane-of-candida-albicans-an-in-vivo-epr-study
#13
Ágnes Blaskó, Zoltán Gazdag, Pál Gróf, Gábor Máté, Szilvia Sárosi, Judit Krisch, Csaba Vágvölgyi, Lilla Makszin, Miklós Pesti
The effects of clary sage (Salvia sclarea L.) oil (CS-oil), and its two main components, linalool (Lol) and linalyl acetate (LA), on cells of the eukaryotic human pathogen yeast Candida albicans were studied. Dynamic and thermodynamic properties of the plasma membrane were investigated by electron paramagnetic resonance (EPR) spectroscopy, with 5-doxylstearic acid (5-SASL) and 16-SASL as spin labels. The monitoring of the head group regions with 5-SASL revealed break-point frequency decrease in a temperature dependent manner of the plasma membrane between 9...
November 8, 2016: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/27791071/climatic-and-evolutionary-drivers-of-phase-shifts-in-the-plague-epidemics-of-colonial-india
#14
Joseph A Lewnard, Jeffrey P Townsend
Immune heterogeneity in wild host populations indicates that disease-mediated selection is common in nature. However, the underlying dynamic feedbacks involving the ecology of disease transmission, evolutionary processes, and their interaction with environmental drivers have proven challenging to characterize. Plague presents an optimal system for interrogating such couplings: Yersinia pestis transmission exerts intense selective pressure driving the local persistence of disease resistance among its wildlife hosts in endemic areas...
October 24, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27789646/complete-genome-sequence-of-pigmentation-negative-yersinia-pestis-strain-cadman
#15
Sean Lovett, Kitty Chase, Galina Koroleva, Gustavo Palacios, David Rozak, Jason T Ladner
Here, we report the genome sequence of Yersinia pestis strain Cadman, an attenuated strain lacking the pgm locus. Y. pestis is the causative agent of plague and generally must be worked with under biosafety level 3 (BSL-3) conditions. However, strains lacking the pgm locus are considered safe to work with under BSL-2 conditions.
October 27, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27745954/intranasal-delivery-of-a-protein-subunit-vaccine-using-a-tobacco-mosaic-virus-platform-protects-against-pneumonic-plague
#16
Paul M Arnaboldi, Mariya Sambir, Christina D'Arco, Lauren A Peters, Jos F M L Seegers, Lloyd Mayer, Alison A McCormick, Raymond J Dattwyler
Yersinia pestis, one of history's deadliest pathogens, has killed millions over the course of human history. It has attributes that make it an ideal choice to produce mass casualties and is a prime candidate for use as a biological weapon. When aerosolized, Y. pestis causes pneumonic plague, a pneumonia that is 100% lethal if not promptly treated with effective antibiotics. Currently, there is no FDA approved plague vaccine. The current lead vaccine candidate, a parenterally administered protein subunit vaccine comprised of the Y...
November 11, 2016: Vaccine
https://www.readbyqxmd.com/read/27744508/acting-on-actin-rac-and-rho-played-by-yersinia
#17
Martin Aepfelbacher, Manuel Wolters
Pathogenic bacteria of the genus Yersinia include Y. pestis-the agent of plaque-and two enteropathogens, Y. enterocolitica, and Y. pseudotuberculosis. These pathogens have developed an array of virulence factors aimed at manipulating Rho GTP-binding proteins and the actin cytoskeleton in host cells to cross the intestinal barrier and suppress the immune system. Yersinia virulence factors include outer membrane proteins triggering cell invasion by binding to integrins, effector proteins injected into host cells to manipulate Rho protein functions and a Rho protein-activating exotoxin...
October 16, 2016: Current Topics in Microbiology and Immunology
https://www.readbyqxmd.com/read/27726806/a-personal-view-of-how-paleomicrobiology-aids-our-understanding-of-the-role-of-lice-in-plague-pandemics
#18
Didier Raoult
We have been involved in the field of paleomicrobiology since 1998, when we used dental pulp to identify Yersinia pestis as the causative agent of the great plague of Marseille (1720). We recently designed a specific technique, "suicide PCR," that can prevent contamination. A controversy arose between two teams, with one claiming that DNA must be altered to amplify it and the other group claiming that demographic data did not support the role of Y. pestis in the Black Death (i.e., the great plague of the Middle Ages)...
August 2016: Microbiology Spectrum
https://www.readbyqxmd.com/read/27722871/perspectives-on-yersinia-pestis-a-model-for-studying-zoonotic-pathogens
#19
Ruifu Yang, Yujun Cui, Yujing Bi
Yersinia pestis is a typical zoonotic bacterial pathogen. The following reasons make this pathogen a model for studying zoonotic pathogens: (1) Its unique lifestyle makes Y. pestis an ideal model for studying host-vector-environment-pathogen interactions; (2) population diversity characters in Y. pestis render it a model species for studying monomorphic bacterial evolution; (3) the pathogenic features of bacteria provide us with good opportunities to study human immune responses; (4) typical animal and vector models of Y...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27722870/bacteriophages-of-yersinia-pestis
#20
Xiangna Zhao, Mikael Skurnik
Bacteriophage play many varied roles in microbial ecology and evolution. This chapter collates a vast body of knowledge and expertise on Yersinia pestis phages, including the history of their isolation and classical methods for their isolation and identification. The genomic diversity of Y. pestis phage and bacteriophage islands in the Y. pestis genome are also discussed because all phage research represents a branch of genetics. In addition, our knowledge of the receptors that are recognized by Y. pestis phage, advances in phage therapy for Y...
2016: Advances in Experimental Medicine and Biology
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