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high grade squamous intraepithelial lesion

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https://www.readbyqxmd.com/read/29328473/detection-of-high-grade-neoplasia-in-air-dried-cervical-pap-smears-by-a-microrna-based-classifier
#1
Mikhail K Ivanov, Sergei E Titov, Sergei A Glushkov, Victoria V Dzyubenko, Anastasia V Malek, Polina A Arkhangelskaya, Roman B Samsonov, Andrey A Mikhetko, Elena V Bakhidze, Igor V Berlev, Nikolay N Kolesnikov
Recent studies have shown that changes in the expression levels of certain microRNAs correlate with the degree of severity of cervical lesions. The aim of the present study was to develop a microRNA-based classifier for the detection of high-grade cervical intraepithelial neoplasia (CIN ≥2) in cytological samples from patients with different high-risk human papillomavirus (HR-HPV) viral loads. For this purpose, raw RT-qPCR data for 25 candidate microRNAs, U6 snRNA and human DNA in air-dried PAP smears from 174 women with different cervical cytological diagnoses, 144 of which were HR-HPV-positive [40 negative for intraepithelial lesion or malignancy (NILM), 34 low-grade squamous intraepithelial lesions (L-SIL), 57 high-grade squamous intraepithelial lesions (H-SIL), 43 invasive cancers], were statistically processed...
January 12, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29314149/uniplex-e6-e7-pcr-method-detecting-e6-or-e7-genes-in-39-human-papillomavirus-types
#2
Mitsuaki Okodo, Kaori Okayama, Koji Teruya, Toshiyuki Sasagawa
We have developed a new human papillomavirus (HPV) assay using the uniplex E6/E7 polymerase chain reaction (PCR) method, which is able to detect E6 and E7 genes in 39 HPV types. We validated the assay for sensitivity and specificity using cloned HPV DNA and clinical samples. A comparative study using Genosearch-31 (GS-31) to determine HPV genotypes in clinical samples was also performed. E6 or E7 genes, measured by uniplex E6/E7 PCR, were detectable in 15 low-risk (HPV-6, -11, -40, -42, -44, -54, -55, -61, -62, -71, -74, -81, -84, -89, -90), 11 intermediate-risk (HPV-26, -30, -34, -53, -66, -67, -69, -70, -73, -82, -85), and 13 high-risk (HR) HPV types...
January 4, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29286606/human-papilloma-virus-genotype-distribution-in-cervical-lesions-in-zanjan-iran
#3
Shahrzad Ahmadi, Hossein Goudarzi, Ahmad Jalilvand, Abdolreza Esmaeilzadeh
Objective: Cervical cancer is one of the most common cancers among women all over the world, and main cause is persistent infection with high risk human papillomavirus (HPV) strains. It has been reported that the distribution and prevalence of HPV types varies by geographical region, so that this is important for prevention by type-specific vaccines. The aim of current study was to determine the genotype distribution of HPV using the INNO-LiPA genotyping assay in Zanjan province, North West Iran. Methods: A total of 112 formalin-fixed paraffin embedded (FFPE) tissue samples from cases of low-grade intraepithelial lesion (LSIL), high-grade intraepithelial lesion (HSIL) and squamous cell carcinoma (SCC) were collected...
December 29, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/29285369/clinical-significance-of-atypical-squamous-cells-of-undetermined-significance-after-treatment-for-cervical-intraepithelial-grade-3-neoplasia-a-retrospective-single-center-cohort-study
#4
Toshimichi Onuma, Kimihisa Tajima, Kumiko Sato, Katsushige Hattori, Shin Fukuda, Takahiro Tsuji, Yoshio Yoshida
The aim of the present study was to evaluate the clinical significance of atypical squamous cells of undetermined significance (ASC-US) following cervical conization for cervical intraepithelial neoplasia (CIN) grade 3. This study was a retrospective cohort analysis. The medical records of women treated with conization for CIN 2-3 were reviewed and 142 patients with CIN 3 who had been diagnosed using the conization specimens were selected. The mean follow-up period after conization was 41.8 months. Cytological abnormalities after conization were observed in 19...
December 2017: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/29279588/comparison-of-the-sensitivity-and-specificity-of-p16-ki-67-dual-staining-and-hpv-dna-testing-of-abnormal-cervical-cytology-in-the-detection-of-histology-proven-cervical-intraepithelial-neoplasia-grade-2-and-above-cin-2
#5
T K Y Tay, K L Lim, M H Hilmy, A A Thike, S T Goh, L H Song, J S G Hwang, S Mantoo
INTRODUCTION: Human papillomavirus (HPV) testing is used as a means of triaging cervico-vaginal smears with low grade squamous abnormalities or as part of co-testing with cytology. While HPV testing has a high sensitivity, it has a low specificity in detecting cervical intraepithelial neoplasia grade 2 and above (CIN 2+) leading to unnecessary colposcopy referrals. We investigate the accuracy of the p16/Ki-67 dual immunocytochemical stain in determining the presence of CIN 2+ lesions on histology and its potential as a superior biomarker for triage...
December 2017: Malaysian Journal of Pathology
https://www.readbyqxmd.com/read/29258466/cervical-cytology-and-human-papillomavirus-among-asymptomatic-healthy-volunteers-in-vientiane-lao-pdr
#6
Reika Takamatsu, Viengvansay Nabandith, Vatsana Pholsena, Phouthasone Mounthisone, Katsu Nakasone, Kentarou Ohtake, Naoki Yoshimi
BACKGROUND: Cervical cancer is the most common cancer in women living in Vientiane, Lao People's Democratic Republic (PDR). This study examines cervical cytology using a liquid-based cytology (LBC) method and reports the presence of high-risk (HR) human papillomavirus (HPV). METHODS: We collected cervical samples from 1475 asymptomatic and healthy volunteers from six hospitals in Lao PDR. A total of 1422 volunteers (mean age 39.1 ± 6.4 years, range 30-54 years) were included in the final analysis...
December 19, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29247086/risk-of-high-grade-lesions-after-atypical-glandular-cells-in-cervical-screening-a-population-based-cohort-study
#7
Ingrid Norman, Anders Hjerpe, Joakim Dillner
OBJECTIVES: To determine how human papillomavirus (HPV) positivity of atypical glandular cells (AGCs) affects the predictive values for the presence of high-grade cervical lesions. DESIGN: Population-based cohort study. SETTING: Stockholm-Gotland region, Sweden. PARTICIPANTS: Between 17 February 2014 and 30 June 2016, there were 562 women with AGC detected in a cervical sample. Registry linkages up to 30 June 2016 identified 392 women with an associated HPV test and a histopathological follow-up...
December 14, 2017: BMJ Open
https://www.readbyqxmd.com/read/29245055/high-proportion-of-abnormal-pap-smear-tests-and-cervical-dysplasia-in-women-with-cystic-fibrosis
#8
Christine Rousset-Jablonski, Quitterie Reynaud, Raphaëlle Nove-Josserand, Isabelle Ray-Coquard, Yahia Mekki, François Golfier, Isabelle Durieu
OBJECTIVES: Insufficient gynecological follow-up and cervical screening has been reported in women with cystic fibrosis (CF). Some of these patients will require a pulmonary transplantation, known to be associated with a higher risk of cervical dysplasia. The aim of this study was to explore the results of cervical screening in adult women with CF, and to report the prevalence of abnormal pap smear tests in this population. STUDY DESIGN: We retrospectively analyzed medical records of sexually active women with CF who attended a gynecological consultation in Lyon University CF referral center between June 2014 and December 2015...
December 9, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29239064/case-of-rapidly-progressing-condylomatous-squamous-cell-carcinoma-of-the-uterine-cervix-associated-with-low-risk-human-papillomavirus-type-6
#9
Miho Masuda, Kaoru Abiko, Sachiko Minamiguchi, Ryusuke Murakami, Tsukasa Baba, Ikuo Konishi
Condylomatous, or warty squamous cell carcinoma (SCC) of the uterine cervix, is a rare variant of invasive SCC. Here, we describe a case of aggressive condylomatous SCC of the uterine cervix. A 43-year-old woman was monitored for 3 years for suspected low-grade squamous intraepithelial lesion. A whitish papillary mass occupied the cervix, and the colposcopic diagnosis was condyloma acuminatum. A cervical biopsy revealed papillary proliferating thick squamous epithelium, which consisted of koilocytes and atypical cells with enlarged nuclei...
December 14, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/29230975/negative-pap-tests-in-women-with-high-grade-cervical-lesions-on-follow-up-biopsies-contributing-factors-and-role-of-human-papillomavirus-genotyping
#10
Steven Goodman, Roxanne R Mody, Donna Coffey, Blythe K Gorman, Eric Luna, Donna Armylagos, Mary R Schwartz, Dina R Mody, Yimin Ge
BACKGROUND: Previous studies have indicated that negative Papanicolaou (Pap) tests can precede high-grade cervical lesions (HGCL) on biopsy. This study aims to determine the contributing factors for cytologic discrepancy and the potential role of human papilloma virus (HPV) testing in risk evaluation of women with negative Pap tests. METHODS: Of 42,797 Pap tests interpreted as negative for intraepithelial lesion or malignancy (NILM) from March 1, 2013 to December 30, 2014, 426 had available HPV testing and follow-up biopsy...
December 12, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/29223946/-quality-of-the-exo-endocervical-sampling-in-the-prevention-of-uterine-cervix-cancer
#11
Anabela A Angeleri, Lili B Díaz, Gisela Coliva, Fernando Guerra, Luis A Palaoro, Adriana E Rocher
Cervical and vaginal cytology, Papanicolaou test (PAP), is the most effective test for screening of preneoplastic lesions, and cervical cancer prevention. Its sensitivity goes from 50 to 98%, according to different statistics, and this variation is related to the sampling procedure. A satisfactory smear should be taken from the transformation zone, the junction between endocervix and exocervix. According to Bethesda, metaplastic and/or endocervical cells should be observed under the microscope. The traditional PAP smear includes an exo-endocervical sampling using the Ayre spatula; however, only near 50% of the smears are representative of the transformation zone...
2017: Medicina
https://www.readbyqxmd.com/read/29215211/a-study-of-hormonal-effects-in-cervical-smear-samples-using-raman-spectroscopy
#12
Damien Traynor, Padraig Kearney, Ines Ramos, Cara M Martin, John J O'Leary, Fiona M Lyng
Raman spectroscopy is a powerful tool that has the potential to be used as a screening method for cervical cancer. It is a label free, low cost method providing a biochemical fingerprint of a given sample. The objective of this study was to address patient to patient variability contributed by hormonal effects due to the menstrual cycle, the use of hormone based contraceptives and the onset of menopause, and to determine if these changes would affect the ability to successfully identify dyskaryotic cells. Raman spectra were recorded from unstained ThinPrep cervical samples (45 cytology negative and 15 high grade dyskaryosis (high grade squamous intraepithelial lesion (SIL)) samples using a HORIBA Jobin Yvon XploRA™ system...
December 7, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/29210158/screening-for-human-papillomavirus-cervical-cytological-abnormalities-and-associated-risk-factors-in-hiv-positive-and-hiv-negative-women-in-rwanda
#13
M F Mukanyangezi, V Sengpiel, O Manzi, G Tobin, S Rulisa, E Bienvenu, D Giglio
OBJECTIVES: Cervical cancer is the major cause of death from cancer in Africa. We wanted to assess the prevalence of human papillomavirus (HPV) infections and associated risk factors and to determine whether HPV testing could serve as a screening method for squamous intraepithelial lesions (SILs) in Rwanda. We also wanted to obtain a broader understanding of the underlying risk factors for the establishment of HPV infection in Rwanda. METHODS: A total of 206 HIV-positive women, 172 HIV-negative women and 22 women with unknown HIV status were recruited at the University Teaching Hospitals of Kigali (UTHK) and of Butare (UTHB) in Rwanda...
December 6, 2017: HIV Medicine
https://www.readbyqxmd.com/read/29208455/high-prevalence-and-incidence-of-hpv-related-anal-cancer-precursor-lesions-in-hiv-positive-women-in-the-late-haart-era
#14
Carmen Hidalgo-Tenorio, Samantha E de Jesus, Javier Esquivias, Juan Pasquau
INTRODUCTION: Anal cancer is one of the most common non-AIDS defining malignancies, especially in men who have sex with men and women living with HIV (WLHIV). OBJECTIVES: To evaluate the prevalence and incidence of precursor lesions (high-grade squamous intraepithelial lesions [HSIL]) and anal cancer in our cohort of women and to compare them to cervical lesions; to calculate the percentage of patients that acquire and clear oncogenic genotypes (HR-HPV) in the anal canal; and to determine predictive factors for anal HPV infection...
December 2, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29207280/hybrid-capture-as-a-tool-for-cervical-lesions-screening-in-hiv-infected-women-insights-from-a-brazilian-cohort
#15
Emilia Moreira Jalil, Paula M Luz, Marcel Quintana, Ruth Khalili Friedman, Rosa M Domingues S Madeira, Angela Cristina Andrade, Janice Chicarino, Ronaldo Ismerio Moreira, Monica Derrico, José Eduardo Levi, Fabio Russomano, Valdilea Gonçalves Veloso, Beatriz Grinsztejn
INTRODUCTION: Cervical cancer remains an important burden for HIV-infected women in the era of combination antiretroviral therapy. Recommendations for cervical screening in these women diverge and may include high-risk HPV (HRHPV) testing. We aimed to evaluate the clinical usefulness of a single HRHPV testing for cervical screening of HIV-infected women. METHODS: 723 HIV-infected women from a Brazilian prospective cohort were included between 1996 and 2012. Inclusion criteria were: normal cervical cytology at baseline and having a HRHPV-test at baseline...
December 2, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29204065/anal-cancer-and-anal-cancer-precursors-in-women-with-a-history-of-hpv-related-dysplasia-and-cancer
#16
Elizabeth A Stier, Elizabeth Y Chiao
The epidemiology of anal cancer in the U.S. has changed over the past 3 decades. During this period the incidence of anal cancer has increased among both men and women. Of note, women with a history of anogenital HPV infection are at higher risk than the general population for anal cancer. The increased risk ranged from increased incidence rate ratios ranging from 1.82 to 6.3 in women with a history of cervical cancer, to 4.2-16.4 in women with a history of prior cervical intraepithelial neoplasia 3 (CIN 3)...
June 2017: Seminars in Colon & Rectal Surgery
https://www.readbyqxmd.com/read/29202724/epcam-expression-in-squamous-cell-carcinoma-of-the-uterine-cervix-detected-by-monoclonal-antibody-to-the-membrane-proximal-part-of-epcam
#17
Warangkana Chantima, Charin Thepthai, Pornsuk Cheunsuchon, Tararaj Dharakul
BACKGROUND: Epithelial cell adhesion molecule (EpCAM) is a promising biomarker for squamous cell carcinoma (SCC) of the uterine cervix, because it is over-expressed in various cancers of epithelial origin. However, EpCAM expression reported in previous immunohistochemistry (IHC) studies was inconsistent. We hypothesize that the membrane-distal part of EpCAM may be lost during tissue preparation, leaving only the membrane-proximal part of EpCAM available for antibody binding and IHC staining...
December 4, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29185272/prevalence-of-human-papillomavirus-genotypes-and-precancerous-cervical-lesions-in-a-screening-population-in-the-republic-of-korea-2014-2016
#18
Yung Taek Ouh, Kyung Jin Min, Hyun Woong Cho, Moran Ki, Jin Kyoung Oh, Sang Yop Shin, Jin Hwa Hong, Jae Kwan Lee
OBJECTIVE: Knowledge regarding the prevalence and distribution of human papillomavirus (HPV) genotyping in healthy women is important in establishing strategies for cervical cancer screening and HPV vaccination. METHODS: A total of 18,170 women who visited a Korean Medical Institute for health check-ups were recruited retrospectively; they underwent HPV genotyping and conventional cervical cytology. An HPV DNA test was performed using the Anyplex™ II HPV 28 detection system (Seegene) or HPV Liquid Bead Microarray (Osang Healthcare)...
January 2018: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/29185266/are-vaginal-swabs-comparable-to-cervical-smears-for-human-papillomavirus-dna-testing
#19
Liselotte Coorevits, Ans Traen, Luc Bingé, Jo Van Dorpe, Marleen Praet, Jerina Boelens, Elizaveta Padalko
OBJECTIVE: Human papillomavirus (HPV) testing is widely incorporated into cervical cancer screening strategies. Current screening requires pelvic examination for cervical sampling, which may compromise participation. The acceptance could be raised by introducing testing on vaginal swabs. We explored the interchangeability of vaginal swabs and cervical smears for HPV testing, by means of a prospective study conducted in female sex workers (FSWs). Besides, we report on the occurrence of 32 different HPV genotypes in FSW with low-grade squamous intraepithelial lesion (LSIL) or high-grade squamous intraepithelial lesion (HSIL)...
January 2018: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/29183133/distribution-of-human-papillomavirus-genotype-in-anal-condyloma-acuminatum-among-japanese-men-the-higher-prevalence-of-high-risk-human-papillomavirus-in-men-who-have-sex-with-men-with-hiv-infection
#20
Satomi Furukawa, Shin Uota, Tetsuo Yamana, Rikisaburo Sahara, Kuniko Iihara, Yoshiyuki Yokomaku, Yasumasa Iwatani, Wataru Sugiura
Human papillomavirus (HPV) infection is known to cause anal condyloma acuminatum (CA) and squamous cell carcinoma. Men who have sex with men (MSM) with human immunodeficiency virus (HIV) infection are frequently co-infected with HPV, especially high risk HPV (HR-HPV) that causes anal squamous cell carcinoma. However, there are few reports of HPV genotype studies in anal lesion of Japanese men. We tried to estimate the distribution of HPV genotypes in anal CA tissue specimens from the Japanese men in order to elucidate the risk of anal cancer...
November 28, 2017: AIDS Research and Human Retroviruses
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