keyword
MENU ▼
Read by QxMD icon Read
search

rb protein

keyword
https://www.readbyqxmd.com/read/28319064/oct4-controls-mitotic-stability-and-inactivates-the-rb-tumor-suppressor-pathway-to-enhance-ovarian-cancer-aggressiveness
#1
E Comisso, M Scarola, M Rosso, S Piazza, S Marzinotto, Y Ciani, M Orsaria, L Mariuzzi, C Schneider, S Schoeftner, R Benetti
OCT4 (Octamer-binding transcription factor 4) is essential for embryonic stem cell self-renewal. Here we show that OCT4 increases the aggressiveness of high-grade serous ovarian cancer (HG-SOC) by inactivating the Retinoblastoma tumor suppressor pathway and enhancing mitotic stability in cancer cells. OCT4 drives the expression of Nuclear Inhibitor of Protein Phosphatase type 1 (NIPP1) and Cyclin F (CCNF) that together inhibit Protein Phosphatase 1 (PP1). This results in pRB hyper-phosphorylation, accelerated cell proliferation and increased in vitro tumorigenicity of ovarian cancer cells...
March 20, 2017: Oncogene
https://www.readbyqxmd.com/read/28302578/effects-of-carbon-sources-and-plant-protein-levels-in-a-biofloc-system-on-growth-performance-and-the-immune-and-antioxidant-status-of-nile-tilapia-oreochromis-niloticus
#2
Abdallah Tageldien Mansour, Maria Ángeles Esteban
The efficacy of different biofloc treatments (BFTs) to compensate for a reduction in dietary protein level under zero-water exchange systems was studied during a 10 weeks experiment, assessing the effect on water quality, growth, immune and antioxidant status of Nile tilapia (Oreochromis niloticus) fingerlings. Six groups were established and fed the same plant-based feed containing 20 or 30% crude protein: two groups in clear water conditions with no added carbon source, two biofloc groups given a wheat milling by-product (WMB) as additional carbon source and two biofloc groups given rice bran (RB)...
March 14, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28300827/simvastatin-and-atorvastatin-inhibit-dna-replication-licensing-factor-mcm7-and-effectively-suppress-rb-deficient-tumors-growth
#3
Juan Li, Jie Liu, Zheyong Liang, Fang He, Lu Yang, Pingping Li, Yina Jiang, Bo Wang, Can Zhou, Yaochun Wang, Yu Ren, Jin Yang, Jianmin Zhang, Zhijun Luo, Cyrus Vaziri, Peijun Liu
Loss or dysfunction of tumor suppressor retinoblastoma (RB) is a common feature in various tumors, and contributes to cancer cell stemness and drug resistance to cancer therapy. However, the strategy to suppress or eliminate Rb-deficient tumor cells remains unclear. In the present study, we accidentally found that reduction of DNA replication licensing factor MCM7 induced more apoptosis in RB-deficient tumor cells than in control tumor cells. Moreover, after a drug screening and further studies, we demonstrated that statin drug Simvastatin and Atorvastatin were able to inhibit MCM7 and RB expressions...
March 16, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28298331/expression-of-leptin-receptor-in-the-oviduct-of-chinese-brown-frog-rana-dybowskii
#4
Liqin Xi, Yuning Liu, Zeqi Tang, Xia Sheng, Haolin Zhang, Qiang Weng, Meiyu Xu
The oviduct of Chinese brown frog (Rana dybowskii) expands specifically during pre-hibernation instead of in the breeding period. In this study, we investigated the expression of leptin receptor (Ob-Rb) in Rana dybowskii oviduct during the breeding period and pre-hibernation. Histologically, the oviduct of Rana dybowskii consists of glandular cells, tubule lumen and epithelial cells. The oviductal weight and pipe diameter also revealed significant differences, which were higher in pre-hibernation than that of the breeding period...
March 15, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/28297760/-promoting-effect-of-cyclin-d1-overexpression-on-proliferation-and-epithelial-mesenchymal-transition-of-cervical-squamous-cell-carcinoma-siha-cells
#5
P Wang, S Liu, B Cheng, X Z Wu, S S Ding, L Xu, Y Liu, L Duan, S Z Sun
Objective: To study effects of cyclin D1 overexpression on the proliferation and differentiation of cervical squamous cell carcinoma SiHa cells and to investigate related signaling molecules. Methods: Primers were designed to amplify the full length of cyclin D1 gene and cyclin D1 gene was amplified by PCR for constructing pcDNA3.1 plasmid vector. The construct was then transfected into SiHa cells, and the cells with stable overexpression of cyclin D1 were established, cyclin D1 gene and protein expression were detected by RT-PCR and Western blot, respectively...
March 8, 2017: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
https://www.readbyqxmd.com/read/28293272/multiple-molecular-interactions-redundantly-contribute-to-rb-mediated-cell-cycle-control
#6
Michael J Thwaites, Matthew J Cecchini, Srikanth Talluri, Daniel T Passos, Jasmyne Carnevale, Frederick A Dick
BACKGROUND: The G1-S phase transition is critical to maintaining proliferative control and preventing carcinogenesis. The retinoblastoma tumor suppressor is a key regulator of this step in the cell cycle. RESULTS: Here we use a structure-function approach to evaluate the contributions of multiple protein interaction surfaces on pRB towards cell cycle regulation. SAOS2 cell cycle arrest assays showed that disruption of three separate binding surfaces were necessary to inhibit pRB-mediated cell cycle control...
2017: Cell Division
https://www.readbyqxmd.com/read/28289097/human-cytomegalovirus-v-cdk-ul97-phosphorylates-and-inactivates-the-retinoblastoma-protein-related-p107-and-p130-proteins
#7
Satoko Iwahori, Angie C Umaña, Halena R VanDeusen, Robert F Kalejta
The human cytomegalovirus (HCMV)-encoded viral cyclin-dependent kinase (v-CDK) UL97 phosphorylates the retinoblastoma (Rb) tumor suppressor. Here we identify the other Rb family members p107 and p130 as novel targets of UL97. UL97 phosphorylates p107 and p130 thereby inhibiting their ability to repress the E2F-responsive E2F1 promoter. As with Rb, this phosphorylation, and the rescue of E2F-responsive transcription, is dependent on the L1 LXCXE motif in UL97 and its interacting clefts on p107 and p130. Interestingly, UL97 does not induce the disruption of all p107-E2F or p130-E2F complexes, as it does to Rb-E2F complexes...
March 13, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28270591/decreased-transcriptional-corepressor-p107-is-associated-with-exercise-induced-mitochondrial-biogenesis-in-human-skeletal-muscle
#8
Debasmita Bhattacharya, Mia Ydfors, Meghan C Hughes, Jessica Norrbom, Christopher G R Perry, Anthony Scimè
Increased mitochondrial content is a hallmark of exercise-induced skeletal muscle remodeling. For this process, considerable evidence underscores the involvement of transcriptional coactivators in mediating mitochondrial biogenesis. However, our knowledge regarding the role of transcriptional corepressors is lacking. In this study, we assessed the association of the transcriptional corepressor Rb family proteins, Rb and p107, with endurance exercise-induced mitochondrial adaptation in human skeletal muscle...
March 2017: Physiological Reports
https://www.readbyqxmd.com/read/28260085/roles-of-immune-inhibitory-molecule-b7-h4-in-cervical-cancer
#9
Sai Han, Yi Li, Jingjing Zhang, Lu Liu, Qian Chen, Qiuhong Qian, Shan Li, Youzhong Zhang
Recent studies have reported that the immune-regulatory protein B7-H4 is highly expressed in various types of cancer, but little is known concerning its roles in cervical cancer. In the present study, we investigated the expression of B7-H4 in human tissues and serum samples, and explored the effects of B7-H4 on proliferation, apoptosis, migration and invasion of cervical cancer cell lines, including SiHa and HeLa. We found that B7-H4 was mainly located in the cytoplasm of cervical cancer cells as determined by immunofluorescence staining...
March 1, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28259945/rb-deficiency-accelerates-progression-of-carcinoma-of-the-urinary-bladder-in-vivo-and-in-vitro-through-inhibiting-autophagy-and-apoptosis
#10
Cheng-Yuan Wang, Zhi-Bin Xu, Jiang-Ping Wang, Yong Jiao, Bo Zhang
Urinary bladder cancer is known as a common cancer diagnosed across the world and results in significant mortality and morbidity rates among patients. The retinoblastoma (Rb) protein, as a main tumor suppressor, controls cellular responses to potentially oncogenic stimulation. Rb phosphorylation could disrupt E2F complex formation, resulting in diverse transcription factor dysfunction. In our study, we investigated how Rb is involved in controlling urinary bladder cancer progression. The results indicate that Rb expression is reduced in mice with urinary bladder tumor, and its suppression leads to urinary bladder cancer progression in vivo and in vitro...
February 22, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28255749/analysis-of-ki67-hmga1-mdm2-and-rb-expression-in-nonfunctioning-pituitary-adenomas
#11
Xiaohui Yao, Hua Gao, Chuzhong Li, Lijuan Wu, Jiwei Bai, Jichao Wang, Yangfang Li, Yazhuo Zhang
Nonfunctioning pituitary adenomas (NFPAs) are the most prevalent type of pituitary macro-adenoma. Clarifying the relationship between NFPA markers and disease progression or recurrence could provide a basis for administration of adjuvant treatments. The present study examined the expression levels of high-mobility group (HMG)A1, Ki-67, mouse double minute 2 homolog (MDM2), and retinoblastoma (RB)with respect to NFPA recurrence. Immunohistochemistry was carried out using antibodies to Ki-67, MDM2, HMGA-1, and RB on tissue microarray slides of a cohort of 35 paired NFPA samples of primary and recurrence/regrowth tumors...
March 2, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/28249908/kinome-wide-rna-interference-screen-reveals-a-role-for-pdk1-in-acquired-resistance-to-cdk4-6-inhibition-in-er-positive-breast-cancer
#12
Valerie M Jansen, Neil E Bhola, Joshua A Bauer, Luigi Formisano, Kyung-Min Lee, Katherine E Hutchinson, Agnieszka K Witkiewicz, Preston D Moore, Monica Valeria Estrada, Violeta Sanchez, Paula G Ericsson, Melinda Sanders, Paula R Pohlmann, Michael J Pishvaian, David A Riddle, Wenyi Wei, Teresa C Dugger, Erik Knudsen, Carlos L Arteaga
To discover mechanisms of resistance to CDK4/6 inhibitors, we used a kinome-wide siRNA screen to identify kinases that, when downregulated, promote sensitivity to ribociclib. We identified 3-phosphoinositide dependent protein kinase 1 (PDK1) as the top siRNA that sensitized ER+ MCF-7 cells to ribociclib. Pharmacological inhibition of PDK1 with GSK2334470 in combination with ribociclib or palbociclib, synergistically inhibited proliferation and increased apoptosis in a panel of ER+ breast cancer cell lines. Ribociclib-resistant MCF-7, T47D and HCC1428 cells, selected after chronic drug exposure, displayed increased levels of PDK1, P-RSK2, P-AKT and P-S6 compared to parental drug-sensitive cells...
March 1, 2017: Cancer Research
https://www.readbyqxmd.com/read/28243323/doxycycline-regulated-p16-mts1-expression-suppresses-the-anchorage-independence-and-tumorigenicity-of-breast-cancer-cell-lines-that-lack-endogenous-p16
#13
Maria C Todd, Thomas A Langan, Robert A Sclafani
The RB pathway controls the critical transition from G1 into S phase of the mammalian cell cycle. Deregulation of the RB pathway by means of RB or p16 inactivation has been implicated in the development of virtually all human cancers. Such findings have led to the view that the loss of RB-mediated regulation at the G1/S checkpoint is a precondition for human malignancy. Our analysis of the RB-positive MCF-7 and ZR75.1 breast cancer cell lines revealed a lack of endogenous p16 protein expression as a result of the homozygous deletion and methylation of the p16 gene at the CDKN2A locus, respectively...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28242412/genomic-instability-and-proliferation-survival-pathways-in-rb1-deficient-malignancies
#14
Lara Pappas, Xiaoliang Leon Xu, David H Abramson, Suresh C Jhanwar
Genomic instability (GIN) is a hallmark of most cancer cells. However, compared to most human cancer cell types, the retinoblastoma tumor cells show a relatively stable genome. The fundamental basis of this genomic stability has yet to be elucidated, and the role of certain proteins involved in cell cycle regulation may be the key to the development of these specific genotypes. We examined whether thyroid hormone receptor beta 1 and 2 (TRβ1 and TRβ2), known to regulate tumorigenesis, and PTTG1, a mitotic checkpoint protein, play a role in maintaining genomic stability in retinoblastoma...
February 8, 2017: Advances in Biological Regulation
https://www.readbyqxmd.com/read/28229086/rb-an-essential-player-in-adult-neurogenesis
#15
Bensun C Fong, Ruth S Slack
The fundamental mechanisms underlying adult neurogenesis remain to be fully clarified. Members of the cell cycle machinery have demonstrated key roles in regulating adult neural stem cell (NSC) quiescence and the size of the adult-born neuronal population. The retinoblastoma protein, Rb, is known to possess CNS-specific requirements that are independent from its classical role as a tumor suppressor. The recent study by Vandenbosch et al. has clarified distinct requirements for Rb during adult neurogenesis, in the restriction of proliferation, as well as long-term adult-born neuronal survival...
2017: Neurogenesis (Austin, Tex.)
https://www.readbyqxmd.com/read/28219226/-effects-of-13-cis-retinoic-acid-combined-with-interferon-%C3%AE-2b-in-mantle-cell-lymphoma-cell-lines-jeko-1-in-vitro
#16
Z B Liu, J J Wen, C G Xu
Objectives: To explore the effects of 13-cis-retinoic acid (13cRA) alone or combined with interferonα-2b (IFNα-2b) for the inhibition of cell growth and apoptosis induction of mantle cell lymphoma cell lines Jeko-1 cells. Methods: Jeko-1 cells were treated by different concentrations of 13cRA alone or combined with IFN-α2b. CCK-8 was used to measure the inhibition effects by different treatments. Cell cycles were analyzed by flow cytometry. Effects on apoptosis were assessed by staining of Annexin Ⅴ/PI...
January 14, 2017: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/28213288/inhibition-of-estrogen-related-receptor-%C3%AE-attenuates-vascular-smooth-muscle-cell-proliferation-and-migration-by-regulating-rhoa-p27-kip1-and-%C3%AE-catenin-wnt4-signaling-pathway
#17
Qunyi Li, Lei Zhu, Liudi Zhang, Haifei Chen, Yingfeng Zhu, Yongli Du, Wanxian Zhong, Mingkang Zhong, Xiaojin Shi
RhoA/p27(Kip1) and β-Catenin/Wnt4 signaling processes play central roles in proliferation and migration in vascular smooth muscle cells (VSMCs). ERRα, a member of orphan nuclear receptors, is a potent prognostic factor in breast, ovarian, colon and other types of tumors. However, biological significance of ERRα in VSMCs as well as the molecular mechanisms remains largely unknown. Therefore, the present study was designed to investigate whether ERRα is involved in the proliferation and migration of VSMCs in vitro and neointimal formation in vivo...
February 14, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28211617/phosphorylation-of-prb-mechanism-for-rb-pathway-inactivation-in-mycn-amplified-retinoblastoma
#18
Kathryn G Ewens, Tricia R Bhatti, Kimberly A Moran, Jennifer Richards-Yutz, Carol L Shields, Ralph C Eagle, Arupa Ganguly
A small, but unique subgroup of retinoblastoma has been identified with no detectable mutation in the retinoblastoma gene (RB1) and with high levels of MYCN gene amplification. This manuscript investigated alternate pathways of inactivating pRb, the encoded protein in these tumors. We analyzed the mutation status of the RB1 gene and MYCN copy number in a series of 245 unilateral retinoblastomas, and the phosphorylation status of pRb in a subset of five tumors using immunohistochemistry. There were 203 tumors with two mutations in RB1 (RB1(-/-) , 83%), 29 with one (RB1(+/-) , 12%) and 13 with no detectable mutations (RB1(+/+) , 5%)...
March 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28203301/progress-with-palbociclib-in-breast-cancer-latest-evidence-and-clinical-considerations
#19
REVIEW
Andrea Rocca, Alessio Schirone, Roberta Maltoni, Sara Bravaccini, Lorenzo Cecconetto, Alberto Farolfi, Giuseppe Bronte, Daniele Andreis
Deregulation of the cell cycle is a hallmark of cancer, and research on cell cycle control has allowed identification of potential targets for anticancer treatment. Palbociclib is a selective inhibitor of the cyclin-dependent kinases 4 and 6 (CDK4/6), which are involved, with their coregulatory partners cyclin D, in the G1-S transition. Inhibition of this step halts cell cycle progression in cells in which the involved pathway, including the retinoblastoma protein (Rb) and the E2F family of transcription factors, is functioning, although having been deregulated...
February 2017: Therapeutic Advances in Medical Oncology
https://www.readbyqxmd.com/read/28197540/an-rb-insensitive-to-cdk-regulation
#20
Manel Joaquin, Eulàlia de Nadal, Francesc Posas
The N-term phosphorylation of Retinoblastoma (RB) by the p38 stress-activated protein kinase (SAPK) makes RB insensitive to cyclin-dependent kinase (CDK)-Cyclin inhibition, which enhances the transcriptional repression of E2F-driven promoters and delays tumor cell growth. This novel mechanism of RB regulation opens up a window for developing new cancer drug treatments for tumors harboring high CDK-Cyclin activity and a wild-type RB gene.
2017: Molecular & Cellular Oncology
keyword
keyword
85938
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"