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https://www.readbyqxmd.com/read/28101580/crude-extract-and-solvent-fractions-of-calystegia-soldanella-induce-g1-and-s%C3%A2-phase-arrest-of-the-cell-cycle-in-hepg2-cells
#1
Jung Im Lee, In-Hye Kim, Taek-Jeong Nam
The representative halophyte Calystegia soldanella (L) Roem. et Schult is a perennial vine herb that grows in coastal dunes throughout South Korea as well as in other regions around the world. This plant has long been used as an edible and medicinal herb to cure rheumatic arthritis, sore throat, dropsy, and scurvy. Some studies have also shown that this plant species exhibits various biological activities. However, there are few studies on cytotoxicity induced by C. soldanella treatment in HepG2 human hepatocellular carcinoma cells...
February 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28099942/bigh3-silencing-increases-retinoblastoma-tumor-growth-in-the-murine-sv40-tag-rb-model
#2
Nathalie Allaman-Pillet, Anne Oberson, Daniel F Schorderet
BIGH3, a secreted protein of the extracellular matrix interacts with collagen and integrins on the cell surface. BIGH3 can have opposing functions in cancer, acting either as tumor suppressor or promoter by enhancing tumor progression and angiogenesis. In the eye, BIGH3 is expressed in the cornea and the retinal pigment epithelium and could impact on the development of retinoblastoma, the most common paediatric intraocular neoplasm. Retinoblastoma initiation requires the inactivation of both alleles of the RB1 tumor suppressor gene in the developing retina and tumor progression involves additional genomic changes...
January 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28099924/microrna-140-mediates-rb-tumor-suppressor-function-to-control-stem-cell-like-activity-through-interleukin-6
#3
Akiyo Yoshida, Shunsuke Kitajima, Fengkai Li, Chaoyang Cheng, Yujiro Takegami, Susumu Kohno, Yuan Song Wan, Naoyuki Hayashi, Hayato Muranaka, Yuuki Nishimoto, Naoko Nagatani, Takumi Nishiuchi, Tran C Thai, Sawako Suzuki, Shinji Nakao, Tomoaki Tanaka, Osamu Hirose, David A Barbie, Chiaki Takahashi
We established an in vitro cell culture system to determine novel activities of the retinoblastoma (Rb) protein during tumor progression. Rb depletion in p53-null mouse-derived soft tissue sarcoma cells induced a spherogenic phenotype. Cells retrieved from Rb-depleted spheres exhibited slower proliferation and less efficient BrdU incorporation, however, much higher spherogenic activity and aggressive behavior. We discovered six miRNAs, including mmu-miR-18a, -25, -29b, -140, -337, and -1839, whose expression levels correlated tightly with the Rb status and spherogenic activity...
January 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28088735/long-non-coding-rna-ccat1-promotes-human-retinoblastoma-so-rb50-and-y79-cells-through-negative-regulation-of-mir-218-5p
#4
Hongxu Zhang, Jianguang Zhong, Zhenyu Bian, Xiang Fang, You Peng, Yongping Hu
OBJECTIVE: To investigate the regulatory role and potential mechanism of long non-coding RNAs (lncRNA) in human retinoblastoma (RB). METHODS: The lncRNA profile in RB tissues were analyzed by microarray and quantitative reverse transcription PCR (qRT-PCR). One of the identified lncRNAs (LncRNA CCAT1) was selected for further experiments. SO-RB50 and Y79 cells were transfected with negative control, siRNA targeting lncRNA CCAT1 (si-CCAT1) and si-CCAT1+miR218-5p inhibitor, respectively...
January 12, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28076568/immunohistochemical-analysis-of-retinoblastoma-cell-phenotype-using-neuronal-and-glial-cell-markers
#5
María Eugenia Orellana, Rubens Belfort, Emilia Antecka, Miguel Noel Burnier
Purpose: The cellular origin of retinoblastoma is uncertain as constituent tumor cells heterogeneously express markers of both immature and mature retinal cells. An immunohistochemical analysis of cellular origin may yield valuable insights into disease progression and treatment options. This study aimed to determine the cellular origin of retinoblastoma in a large case series and correlate these findings with histopathological prognostic factors. Methods: Thirty-nine retinoblastoma cases were histopathologically diagnosed and analyzed by immunohistochemistry using monoclonal antibodies against the immature neural cell marker SRY-box containing gene 2 (SOX-2), the mature neuronal cell marker microtubule-associated protein 2 (MAP2), and the mature glial cell marker glial fibrillary acidic protein (GFAP)...
November 2016: Arquivos Brasileiros de Oftalmologia
https://www.readbyqxmd.com/read/28068625/loss-of-periplakin-expression-is-associated-with-the-tumorigenesis-of-colorectal-carcinoma
#6
Xiang Li, Guohui Zhang, Yan Wang, Ahmed Elgehama, Yang Sun, Lele Li, Yanhong Gu, Wenjie Guo, Qiang Xu
Periplakin (PPL), a member of the plakin protein family, has been reported to be down-expressed in urothelial carcinoma. The role of PPL in human colorectal cancer, however, remains largely unknown. Also little is known about the contribution of PPL to the malignant property of colorectal cancer and the intracellular function of PPL. In this study, we demonstrated that PPL was apparently down-expressed in colon carcinomas compared with normal and para-carcinoma tissues, which was correlated with the tumor size...
January 6, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28050229/dual-function-of-mdm2-and-mdmx-toward-the-tumor-suppressors-p53-and-rb
#7
REVIEW
Jesús Hernández-Monge, Adriana Berenice Rousset-Roman, Ixaura Medina-Medina, Vanesa Olivares-Illana
The orchestrated crosstalk between the retinoblastoma (RB) and p53 pathways contributes to preserving proper homeostasis within the cell. The deregulation of one or both pathways is a common factor in the development of most types of human cancer. The proto-oncoproteins MDMX and MDM2 are the main regulators of the well- known tumor suppressor p53 protein. Under normal conditions, MDM2 and MDMX inhibit p53, either via repression of its transcriptional activity by protein-protein interaction, or via polyubiquitination as a result of MDM2-E3 ubiquitin ligase activity, for which MDM2 needs to dimerize with MDMX...
September 2016: Genes & Cancer
https://www.readbyqxmd.com/read/28050067/palbociclib-a-breakthrough-in-breast-carcinoma-in-women
#8
REVIEW
Ajay Kumar Gupta, Sushil Sharma, Navdeep Dahiya, D B S Brashier
Breast cancer (BC) is the most common cancer and leading cause of death in women worldwide. Cellular proliferation, growth, and division are tightly controlled by the cell-cycle regulatory machinery. An important pathway is cyclin-dependent kinases (CDKs) which regulate cell cycle and thus control transcriptional processes. In human cancer, multiple CDK family members are commonly deregulated. The cyclin D-CDK4/6-retinoblastoma (RB) protein-INK4 axis is particularly affected in many solid tumors which leads to cancer cell proliferation...
December 2016: Medical Journal, Armed Forces India
https://www.readbyqxmd.com/read/28031113/-genomelab-tm-gexp-analysis-system-to-detect-apoptosis-associated-genes-in-a549-cells-treated-by-recombinant-human-il-24-combined-with-cisplatin
#9
Jinjin Guo, Shaohui Wang, Wanbang Sun, Yanli Tang, Guangbin Jiang, Mingying Song
Objective To investigate the changes of apoptosis-associated genes in human lung adenocarcinoma cell line A549 after treated by the recombinant human interleukin-24 (rhIL-24) combined with cisplatin (DDP). Methods The mRNA levels of Bax, Bcl-2, survivin, caspase-3, retinoblastoma (Rb) gene, p53 were analyzed by GenomeLab(TM) GeXP genetic analysis system after A549 cells were treated with 160 ng/mL rhIL-24 or 3 μg/mL DDP or 160 ng/mL rhIL-24 combined with 3 μg/mL DDP. Results The rhIL-24 up-regulated the levels of Bax, Rb, caspase-3 mRNAs and down-regulated Bcl-2, survivin mRNAs, but the change of p53 mRNA was not regular...
January 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28004068/-up-regulation-of-erbb3-binding-protein-1-inhibits-the-growth-of-esophageal-carcinoma-cells
#10
Hao Jiang, Dong-Ping Liu, Dong Xie, Dong-Zhi Wei
The objective of this study was to investigate the role of ErbB3-binding protein 1 (Ebp1) in the growth of esophageal squamous cell carcinoma (ESCC) cells and the underlying mechanism. Eca109 and KYSE150 cells were transfected with lentiviral vector carrying Ebp1 gene. The mRNA levels of Ebp1 in esophageal cancer tissues and paired adjacent normal tissues were examined by real-time PCR. The growth and viability of esophageal carcinoma cells were assessed using MTT and crystal violet assays, respectively. Clone-forming abilities of Eca109 and KYSE150 cells were analyzed by soft agar assay...
December 25, 2016: Sheng Li Xue Bao: [Acta Physiologica Sinica]
https://www.readbyqxmd.com/read/28002961/design-synthesis-and-biological-activity-of-substrate-competitive-smyd2-inhibitors
#11
Scott D Cowen, Daniel Russell, Leslie A Dakin, Huawei Chen, Nicholas A Larsen, Robert Godin, Scott Throner, Xiaolan Zheng, Audrey Molina, Jiaquan Wu, Tony Cheung, Tina Howard, Renee Garcia-Arenas, Nicholas Keen, Christopher S Pendleton, Jennifer A Pietenpol, Andrew D Ferguson
Protein lysine methyltransferases (KMTs) have emerged as important regulators of epigenetic signaling. These enzymes catalyze the transfer of donor methyl groups from the cofactor S-adenosylmethionine to specific acceptor lysine residues on histones, leading to changes in chromatin structure and transcriptional regulation. These enzymes also methylate an array of nonhistone proteins, suggesting additional mechanisms by which they influence cellular physiology. SMYD2 is reported to be an oncogenic methyltransferase that represses the functional activity of the tumor suppressor proteins p53 and RB...
December 22, 2016: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27998772/zinc-induces-apoptosis-on-cervical-carcinoma-cells-by-p53-dependent-and-independent-pathway
#12
Seog Nyeon Bae, Keun Ho Lee, Jin Hwi Kim, Sung Jong Lee, Lae Ok Park
OBJECTIVE: There is evidence that the mineral zinc is involved in the apoptotic cell death of various carcinoma cells. In this study, we aim to determine whether zinc in the form of CIZAR induces apoptosis in cervical carcinoma cells by increasing intracellular zinc concentration. STUDY DESIGN: CaSki and HeLa cervical carcinoma cells and HPV-16 DNA-transformed keratinocyte (CRL2404) were treated with different concentrations of CIZAR. The cell viability test was carried out, the intracellular level of zinc was determined, and apoptosis was confirmed by flow cytometry after propidium iodide (PI) staining and fluorescence microscopy under DAPI staining...
December 17, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27992230/ph-and-potential-transients-of-the-bc1-complex-co-reconstituted-in-proteo-lipobeads-with-the-reaction-center-from-rb-sphaeroides
#13
Andreas F Geiss, Raghav Khandelwal, Dieter Baurecht, Christina Bliem, Ciril Reiner-Rozman, Michael Boersch, G Matthias Ullmann, Leslie M Loew, Renate L C Naumann
His-tag technology is employed to bind membrane proteins, such as the bc1 complex and the reaction center (RC) from Rhodobacter sphaeroides, to spherical as well as planar surfaces in a strict orientation. Subsequently, the spherical and planar surfaces are subjected to in situ dialysis to form proteo-lipobeads (PLBs) and protein-tethered bilayer membranes, respectively. PLBs based on Ni-nitrileotriacetic acid-functionalized agarose beads that have diameters ranging from 50 to 150 μm are used to assess proton release and membrane potential parameters by confocal laser-scanning microscopy...
January 4, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/27991962/the-complex-link-between-apoptosis-and-autophagy-a-promising-new-role-for-rb
#14
João M A Delou, Deborah Biasoli, Helena L Borges
Physiological processes, as autophagy, proliferation and apoptosis are affected during carcinogenesis. Restoring cellular sensitivity to apoptotic stimuli, such as the antineoplastic cocktails, has been explored as a strategy to eliminate cancer cells. Autophagy, a physiological process of recycling organelles and macromolecules can be deviated from homeostasis to support cancer cells survival, proliferation, escape from apoptosis, and therapy resistance. The relationship between autophagy and apoptosis is complex and many stimuli can induce both processes...
October 2016: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/27983729/genetic-screening-in-iranian-patients-with-retinoblastoma
#15
K Shahraki, A Ahani, P Sharma, M Faranoush, G Bahoush, I Torktaz, W A Gahl, M Naseripour, B Behnam
PurposeThe most common intraocular tumor in childhood, retinoblastoma, is largely associated with mutations in the RB1 gene. In the most comprehensive RB1 screening in Iran, we evaluated the RB1 mutations in 106 patients with retinoblastoma, including 73 bilateral (heritable) and 33 unilateral (sporadic) cases.Patients and methodsMutations were identified using amplification refractory mutation system (ARMS) PCR and direct sequencing of the 27 coding exons of RB1 and multiplex ligation-dependent probe amplification (MLPA)...
December 16, 2016: Eye
https://www.readbyqxmd.com/read/27966456/ablating-all-three-retinoblastoma-family-members-in-mouse-lung-leads-to-neuroendocrine-tumor-formation
#16
Sara Lázaro, Miriam Pérez-Crespo, Ana Belén Enguita, Pilar Hernández, Jesús Martínez-Palacio, Marta Oteo, Julien Sage, Jesús M Paramio, Mirentxu Santos
Lung cancer is a deadly disease with increasing cases diagnosed worldwide and still a very poor prognosis. While mutations in the retinoblastoma (RB1) tumor suppressor have been reported in lung cancer, mainly in small cell lung carcinoma, the tumor suppressive role of its relatives p107 and p130 is still a matter of debate. To begin to investigate the role of these two Rb family proteins in lung tumorigenesis, we have generated a conditional triple knockout mouse model (TKO) in which the three Rb family members can be inactivated in adult mice...
December 10, 2016: Oncotarget
https://www.readbyqxmd.com/read/27965693/comprehensive-analysis-of-photosynthetic-characteristics-and-quality-improvement-of-purple-cabbage-under-different-combinations-of-monochromatic-light
#17
Biyun Yang, Xiangzhu Zhou, Ru Xu, Jin Wang, Yizhang Lin, Jie Pang, Shuang Wu, Fenglin Zhong
Light is essential for plant growth. Light intensity, photoperiod, and light quality all affect plant morphology and physiology. Compared to light intensity, photoperiod, little is known about the effects of different monochromatic lights on crop species. To investigate how different lighting conditions influence crops with heterogeneous colors in leaves, we examined photosynthetic characteristics and quality (regarding edibility and nutrition) of purple cabbage under different combinations of lights. Eight different treatments were applied including monochromic red (R), monochromic blue (B), monochromic yellow (Y), monochromic green (G), and the combination of red and blue (3/1, RB), red/blue/yellow (3/1/1, RBY), red/blue/green (3/1/1,RBG), and white light as the control...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27965688/mutations-in-rice-yellow-mottle-virus-polyprotein-p2a-involved-in-rymv2-gene-resistance-breakdown
#18
Agnès Pinel-Galzi, Christine Dubreuil-Tranchant, Eugénie Hébrard, Cédric Mariac, Alain Ghesquière, Laurence Albar
Rice yellow mottle virus (RYMV) is one of the major diseases of rice in Africa. The high resistance of the Oryza glaberrima Tog7291 accession involves a null allele of the RYMV2 gene, whose ortholog in Arabidopsis, CPR5, is a transmembrane nucleoporin involved in effector-triggered immunity. To optimize field deployment of the RYMV2 gene and improve its durability, which is often a weak point in varietal resistance, we analyzed its efficiency toward RYMV isolates representing the genetic diversity of the virus and the molecular basis of resistance breakdown...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27940353/white-matter-damage-and-glymphatic-dysfunction-in-a-model-of-vascular-dementia-in-rats-with-no-prior-vascular-pathologies
#19
Poornima Venkat, Michael Chopp, Alex Zacharek, Chengcheng Cui, Li Zhang, Qingjiang Li, Mei Lu, Talan Zhang, Amy Liu, Jieli Chen
We investigated cognitive function, axonal/white matter (WM) changes and glymphatic function of vascular dementia using a multiple microinfarction (MMI) model in retired breeder (RB) rats. The MMI model induces significant (p < 0.05) cognitive decline that worsens with age starting at 2 weeks, which persists until at least 6 weeks after MMI. RB rats subjected to MMI exhibit significant axonal/WM damage identified by decreased myelin thickness, oligodendrocyte progenitor cell numbers, axon density, synaptic protein expression in the cortex and striatum, cortical neuronal branching, and dendritic spine density in the cortex and hippocampus compared with age-matched controls...
February 2017: Neurobiology of Aging
https://www.readbyqxmd.com/read/27936046/the-ras-effector-interface-isoform-specific-differences-in-the-effector-binding-regions
#20
Hossein Nakhaeizadeh, Ehsan Amin, Saeideh Nakhaei-Rad, Radovan Dvorsky, Mohammad Reza Ahmadian
RAS effectors specifically interact with the GTP-bound form of RAS in response to extracellular signals and link them to downstream signaling pathways. The molecular nature of effector interaction by RAS is well-studied but yet still incompletely understood in a comprehensive and systematic way. Here, structure-function relationships in the interaction between different RAS proteins and various effectors were investigated in detail by combining our in vitro data with in silico data. Equilibrium dissociation constants were determined for the binding of HRAS, KRAS, NRAS, RRAS1 and RRAS2 to both the RAS binding (RB) domain of CRAF and PI3Kα, and the RAS association (RA) domain of RASSF5, RALGDS and PLCε, respectively, using fluorescence polarization...
2016: PloS One
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