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https://www.readbyqxmd.com/read/29783052/the-effect-of-the-supporting-electrolyte-on-the-electrooxidation-of-enrofloxacin-using-a-flow-cell-with-a-bdd-anode-kinetics-and-follow-up-of-oxidation-intermediates-and-antimicrobial-activity
#1
Jussara F Carneiro, José M Aquino, Adilson J Silva, Juliana C Barreiro, Quezia B Cass, Romeu C Rocha-Filho
The role of the supporting electrolyte - SE (Na2 SO4 ; NaCl; Na2 CO3 ; NaNO3 ; Na3 PO4 - 0.1 M ionic strength) in the galvanostatic (10 mA cm-2 ) electrochemical degradation of the fluoroquinolone antibiotic enrofloxacin (ENRO; 100 mg L-1 ) using a filter-press flow cell with a boron-doped diamond anode was investigated (flow rate, solution volume, and temperature were kept fixed at 420 L h-1 , 1.0 L, and 25 °C, respectively). The electrochemical degradation performance with the different SEs was assessed by following up [ENRO], total organic carbon concentration (TOC), oxidation intermediates (detected by LC and LC-QqTOF), and antimicrobial activity towards Escherichia coli as the electrolyses progressed...
May 10, 2018: Chemosphere
https://www.readbyqxmd.com/read/29783048/effect-of-auriculotherapy-on-the-plasma-concentration-of-biomarkers-in-individuals-with-knee-osteoarthritis
#2
Rebeca Graça Costa-Cavalcanti, Danúbia da Cunha de Sá-Caputo, Eloá Moreira-Marconi, Cristiane Ribeiro Kütter, Samuel Brandão-Sobrinho-Neto, Laisa Liane Paineiras-Domingos, Marcia Cristina Moura-Fernandes, João Marcelo Castelpoggi da Costa, José Maurício de Morais Carmo, José Firmino Nogueira-Neto, Shyang Chang, Mario Bernardo-Filho
Knee osteoarthritis (KOA) is one of the most frequent non-communicable diseases with pain associated symptoms and affects the musculoskeletal system. Various forms of treatment can be indicated and non-pharmacological treatment is also an available option for the management of KOA individuals. For instance, auriculotherapy (AT) is one possible procedure associated with the Traditional Chinese Medicine for dealing with KOA. It is believed that the concentration of certain biomarkers could be altered in individuals with KOA after AT...
May 18, 2018: Journal of Acupuncture and Meridian Studies
https://www.readbyqxmd.com/read/29783047/stroma-cell-derived-factor-1-and-connexins-37-and-43-are-preserved-after-vitrification-and-in-vitro-culture-of-goat-ovarian-cortex
#3
Nathalie Jiatsa Donfack, Kele Amaral Alves, Benner Geraldo Alves, Rebeca Magalhães Pedrosa Rocha, Jamily Bezzera Bruno, Marcelo Bertolini, Regiane Rodrigues Dos Santos, Sheyla Farhaydes Souza Domingues, José Ricardo De Figueiredo, Johan Smitz, Ana Paula Ribeiro Rodrigues
This study aimed to evaluate the follicular morphology and development (follicular activation, cell proliferation, and hormone production), as well as the distribution pattern of Connexins 37 and 43 and SDF-1α after vitrification and in vitro culture of goat ovarian tissue. The study involved four experimental groups: fresh control, vitrified control, fresh culture and vitrified culture. The ovarian fragments were vitrified by a solid surface technique using the Ovarian Tissue Cryosystem and subsequently in vitro cultured for 7 days...
May 12, 2018: Theriogenology
https://www.readbyqxmd.com/read/29782955/loss-of-calretinin-and-parvalbumin-positive-interneurones-in-the-hippocampal-ca1-of-aged-alzheimer-s-disease-mice
#4
Fatima Zallo, Emanuela Gardenal, Alexei Verkhratsky, José Julio Rodríguez
Neuronal degeneration associated with Alzheimer's disease (AD), is linked to impaired calcium homeostasis and to changes in calcium-binding proteins (CBPs). The AD-related modification of neuronal CBPs remains controversial. Here we analyzed the presence and expression of calretinin (CR) and parvalbumin (PV) in the hippocampal CA1 neurones of 18 months old 3xTg-AD mice compared to non-Tg animals. We found a layer specific decrease in number of interneurones expressing CR and PV (by 33.7% and 52%, respectively)...
May 18, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29782827/prevalence-and-risk-factors-of-mild-chronic-renal-failure-in-hiv-infected-patients-influence-of-female-gender-and-antiretroviral-therapy
#5
Marina Pontello Cristelli, Joan Carles Trullàs, Federico Cofán, Naira Rico, Christian Manzardo, Juan Ambrosioni, Josep Lluis Bedini, Asunción Moreno, Fritz Diekmann, Jose Maria Miro
BACKGROUND: In people living with HIV, much is known about chronic kidney disease, defined as a glomerular filtration rate under 60mL/min. However, there is scarce data about prevalence and risk factors for milder impairment (60-89mL/min). OBJECTIVE: The present study aims to assess the influence of sex, antiretroviral therapy, and classical risk factors on the occurrence of mild decreased renal function in a large Spanish cohort of HIV-infected patients. METHODS: Cross-sectional, single center study, including all adult HIV-1-infected patients under antiretroviral treatment with at least two serum creatinine measures during 2014, describing the occurrence of and the risk factors for mildly decreased renal function (eGFR by CKD-EPI creatinine equation of 60-89mL/min)...
May 18, 2018: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29782810/chloride-and-sodium-ion-concentrations-in-saliva-and-sweat-as-a-method-to-diagnose-cystic-fibrosis
#6
Aline Cristina Gonçalves, Fernando Augusto Lima Marson, Regina Maria Holanda Mendonça, Carmen Sílvia Bertuzzo, Ilma Aparecida Paschoal, José Dirceu Ribeiro, Antônio Fernando Ribeiro, Carlos Emílio Levy
OBJECTIVE: Cystic fibrosis diagnosis is dependent on the chloride ion concentration in the sweat test (≥60mEq/mL - recognized as the gold standard indicator for cystic fibrosis diagnosis). Moreover, the salivary glands express the CFTR protein in the same manner as sweat glands. Given this context, the objective was to verify the correlation of saliva chloride concentration (SaCl) and sweat chloride concentration (SwCl), and between saliva sodium concentration (SaNa) and sweat sodium concentration (SwNa), in patients with cystic fibrosis and healthy control subjects, as a tool for cystic fibrosis diagnosis...
May 18, 2018: Jornal de Pediatria
https://www.readbyqxmd.com/read/29782657/phantom-clinical-and-texture-indices-evaluation-and-optimization-of-a-penalized-likelihood-image-reconstruction-method-q-clear-on-a-bgo-pet-ct-scanner
#7
Gabriel Reynés-Llompart, Cristina Gámez-Cenzano, José Luís Vercher-Conejero, Aida Sabaté-Llobera, Nahúm Calvo-Malvar, Josep M Martí-Climent
INTRODUCTION: The aim of this study was to evaluate the behavior of a penalized-likelihood image reconstruction method (Q.Clear) under different count statistics and lesion-to-background ratios (LBR) on a BGO scanner, in order to obtain an optimum penalization factor (β value) to study and optimize for different acquisition protocols and clinical goals. METHODS: Both phantom and patient images were evaluated. Data from an image quality phantom were acquired using different Lesion-to-Background ratios and acquisition times...
May 21, 2018: Medical Physics
https://www.readbyqxmd.com/read/29782553/correction-electrocardiographic-biomarkers-for-detection-of-drug-induced-late-sodium-current-block
#8
Jose Vicente, Lars Johannesen, Meisam Hosseini, Jay W Mason, Philip T Sager, Esther Pueyo, David G Strauss
[This corrects the article DOI: 10.1371/journal.pone.0163619.].
2018: PloS One
https://www.readbyqxmd.com/read/29782551/structural-insights-on-the-efficient-catalysis-of-hydroperoxide-reduction-by-ohr-crystallographic-and-molecular-dynamics-approaches
#9
Erika Piccirillo, Thiago G P Alegria, Karen F Discola, José R R Cussiol, Renato M Domingos, Marcos A de Oliveira, Leandro de Rezende, Luis E S Netto, Antonia T-do Amaral
Organic hydroperoxide resistance (Ohr) enzymes are highly efficient Cys-based peroxidases that play central roles in bacterial response to fatty acid hydroperoxides and peroxynitrite, two oxidants that are generated during host-pathogen interactions. In the active site of Ohr proteins, the conserved Arg (Arg19 in Ohr from Xylella fastidiosa) and Glu (Glu51 in Ohr from Xylella fastidiosa) residues, among other factors, are involved in the extremely high reactivity of the peroxidatic Cys (Cp) toward hydroperoxides...
2018: PloS One
https://www.readbyqxmd.com/read/29782512/regulation-of-midgut-cell-proliferation-impacts-aedes-aegypti-susceptibility-to-dengue-virus
#10
Mabel L Taracena, Vanessa Bottino-Rojas, Octavio A C Talyuli, Ana Beatriz Walter-Nuno, José Henrique M Oliveira, Yesseinia I Angleró-Rodriguez, Michael B Wells, George Dimopoulos, Pedro L Oliveira, Gabriela O Paiva-Silva
Aedes aegypti is the vector of some of the most important vector-borne diseases like dengue, chikungunya, zika and yellow fever, affecting millions of people worldwide. The cellular processes that follow a blood meal in the mosquito midgut are directly associated with pathogen transmission. We studied the homeostatic response of the midgut against oxidative stress, as well as bacterial and dengue virus (DENV) infections, focusing on the proliferative ability of the intestinal stem cells (ISC). Inhibition of the peritrophic matrix (PM) formation led to an increase in reactive oxygen species (ROS) production by the epithelial cells in response to contact with the resident microbiota, suggesting that maintenance of low levels of ROS in the intestinal lumen is key to keep ISCs division in balance...
May 21, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29782426/nailfold-videocapillaroscopy-changes-are-associated-with-the-presence-and-severity-of-systemic-sclerosis-related-interstitial-lung-disease
#11
Joana Caetano, Filipe S Paula, Marta Amaral, Susana Oliveira, José D Alves
OBJECTIVE: The aim of this study was to evaluate the association of nailfold videocapillaroscopy (NVC) changes with the presence and severity of interstitial lung disease (ILD) in systemic sclerosis. METHODS: This was a cross-sectional analysis of 48 systemic sclerosis patients (21 patients with ILD). The NVC characteristics considered were capillary organization, capillary loss (CL), avascular areas, enlarged and giant capillaries, hemorrhages, abnormally shaped capillaries, edema, and intermittent flux...
May 19, 2018: Journal of Clinical Rheumatology: Practical Reports on Rheumatic & Musculoskeletal Diseases
https://www.readbyqxmd.com/read/29782315/tlr4-gene-expression-and-pro-inflammatory-cytokines-in-alzheimer-s-disease-and-in-response-to-hippocampal-deafferentation-in-rodents
#12
Justin Miron, Cynthia Picard, Josée Frappier, Doris Dea, Louise Théroux, Judes Poirier
One important aspect in Alzheimer's disease pathology is the presence of chronic inflammation. Considering its role as a key receptor in the microglial innate immune system, TLR4 was shown to regulate the binding and phagocytosis of amyloid plaques by microglia in several mouse models of amyloidosis, as well as the production of pro-inflammatory cytokines. To our knowledge, TLR4 and its association with cytokines have not been thoroughly examined in the brains of subjects affected with Alzheimer's disease. Using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in postmortem human brains, we observed increased expression for the TLR4 and TNF genes (p = 0...
May 16, 2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29782210/colonization-with-enterobacteriaceae-producing-esbls-ampcs-and-oxa-48-in-wild-avian-species-spain-2015-2016
#13
Jesús Oteo, Aida Mencía, Verónica Bautista, Natalia Pastor, Noelia Lara, Fernando González-González, Francisco Javier García-Peña, José Campos
Antibiotic resistance is a global threat of complex and changeable epidemiology. The role of wild birds in the dissemination of antibacterial resistance might be underestimated. We studied the cloacal colonization by cefotaxime-resistant Enterobacteriaceae in 668 wild birds in Spain. Eighty-eight wild birds (13.2%) of 28 species carried cefotaxime-resistant isolates; 58 of them (8.7%) carried extended-spectrum β-lactamases (ESBLs) and 15 (2.5%) plasmid-mediated AmpCs of the blaCIT family. The 58 ESBLs belonged to the CTX-M-1 group (63...
May 21, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29782186/excessive-erythrocytosis-and-cardiovascular-risk-in-andean-highlanders
#14
Noemí Corante, Cecilia Anza-Ramírez, Rómulo Figueroa-Mujíca, José Luis Macarlupú, Gustavo Vizcardo-Galindo, Grzegorz Bilo, Gianfranco Parati, Jorge L Gamboa, Fabiola León-Velarde, Francisco C Villafuerte
Cardiovascular diseases are the main cause of death worldwide. Life under high-altitude (HA) hypoxic conditions is believed to provide highlanders with a natural protection against cardiovascular and metabolic diseases compared with sea-level inhabitants. However, some HA dwellers become intolerant to chronic hypoxia and develop a progressive incapacitating syndrome known as chronic mountain sickness (CMS), characterized by excessive erythrocytosis (EE; Hb ≥21 g/dL in men, Hb ≥19 g/dL in women). Evidence from HA studies suggests that, in addition to CMS typical signs and symptoms, these highlanders may also suffer from metabolic and cardiovascular disorders...
May 21, 2018: High Altitude Medicine & Biology
https://www.readbyqxmd.com/read/29782181/effectiveness-of-an-osteopathic-abdominal-manual-intervention-in-pain-thresholds-lumbopelvic-mobility-and-posture-in-women-with-chronic-functional-constipation
#15
María José Martínez-Ochoa, Juan Carlos Fernández-Domínguez, Jose Miguel Morales-Asencio, Javier González-Iglesias, François Ricard, Ángel Oliva-Pascual-Vaca
OBJECTIVES: To assess the effect of an osteopathic abdominal manual intervention (AMI) on pressure pain thresholds (PPTs), mobility, hip flexibility, and posture in women with chronic functional constipation. DESIGN: Randomized, double-blind placebo-controlled trial. SETTING/LOCATION: Subjects were recruited for the study by referral from different gastroenterology outpatient clinics in the city of Madrid (Spain). SUBJECTS: Sixty-two patients suffering from chronic functional constipation according to the guidelines of the Congress of Rome III...
May 21, 2018: Journal of Alternative and Complementary Medicine: Research on Paradigm, Practice, and Policy
https://www.readbyqxmd.com/read/29782064/accuracy-of-p57-kip-2-compared-with-genotyping-to-diagnose-complete-hydatidiform-mole-a-systematic-review-and-meta-analysis
#16
José Mauro Madi, Antonio Braga, Machline Paim Paganella, Isnard Elman Litvin, Eliana Márcia Wendland
BACK GROUND: Distinguishing hydatidiform moles (HMs) from non-molar specimens and the sub-classification of HM are important because complete hydatidiform mole (CHM) is associated with an increased risk of development of gestational trophoblastic neoplasia. However, diagnosis based solely on morphology has poor interobserver reproducibility. Recent studies have demonstrated that the use of p57KIP 2 immunostaining improves diagnostic accuracy for CHM. OBJECTIVES: To evaluate the accuracy of p57KIP 2 immunostaining compared with molecular genotyping for the diagnosis of CHM...
May 21, 2018: BJOG: An International Journal of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/29782032/leaf-associated-bacterial-microbiota-of-coffee-and-its-correlation-with-manganese-and-calcium-levels-on-leaves
#17
Leandro Pio de de Sousa, Marcio José da da Silva, Jorge Maurício Mondego
Coffee is one of the most valuable agricultural commodities and the plants' leaves are the primary site of infection for most coffee diseases, such as the devastating coffee leaf rust. Therefore, the use of bacterial microbiota that inhabits coffee leaves to fight infections could be an alternative agricultural method to protect against coffee diseases. Here, we report the leaf-associated bacteria in three coffee genotypes over the course of a year, with the aim to determine the diversity of bacterial microbiota...
May 17, 2018: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29781985/testing-the-role-of-multicopy-plasmids-in-the-evolution-of-antibiotic-resistance
#18
Jose Antonio Escudero, R Craig MacLean, Alvaro San Millan
Multicopy plasmids are extremely abundant in prokaryotes but their role in bacterial evolution remains poorly understood. We recently showed that the increase in gene copy number per cell provided by multicopy plasmids could accelerate the evolution of plasmid-encoded genes. In this work, we present an experimental system to test the ability of multicopy plasmids to promote gene evolution. Using simple molecular biology methods, we constructed a model system where an antibiotic resistance gene can be inserted into Escherichia coli MG1655, either in the chromosome or on a multicopy plasmid...
May 2, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29781950/report-of-the-key-opinion-leaders-meeting-on-stem-cell-derived-beta-cells
#19
Jon Odorico, James Markmann, Douglas Melton, Julia Greenstein, Albert Hwa, Cristina Nostro, Alireza Rezania, Jose Oberholzer, Daniel Pipeleers, Luhan Yang, Chad Cowan, Danwei Huangfu, Dieter Egli, Uri Ben-David, Ludovic Vallier, Shane T Grey, Qizhi Tang, Bart Roep, Camilo Ricordi, Ali Naji, Giuseppe Orlando, Daniel G Anderson, Mark Poznansky, Barbara Ludwig, Alice Tomei, Dale L Greiner, Melanie Graham, Melissa Carpenter, Giovanni Migliaccio, Kevin D'Amour, Bernhard Hering, Lorenzo Piemonti, Thierry Berney, Mike Rickels, Thomas Kay, Ann Adams
Beta cell replacement has the potential to restore euglycemia in patients with insulin dependent diabetes. While great progress has been made in establishing allogeneic islet transplantation from deceased donors as the standard of care for those with the most labile diabetes, it is also clear that the deceased donor organ supply cannot possibly treat all those who could benefit from restoration of a normal beta cell mass, especially if immunosuppression were not required. Against this background, the International Pancreas and Islet Transplant Association (IPITA) in collaboration with the Harvard Stem Cell Institute (HSCI), the Juvenile Diabetes Research Foundation (JDRF), and the Helmsley Foundation held a 2-day Key Opinion Leaders Meeting in Boston in 2016 to bring together experts in generating and transplanting beta cells derived from stem cells...
April 30, 2018: Transplantation
https://www.readbyqxmd.com/read/29781804/control-of-the-plasmonic-near-field-in-metallic-nanohelices
#20
José M Caridad, Sinead Winters, David McCloskey, Georg S Duesberg, John F Donegan, Vojislav Krstić
The optical response of metallic nanohelices is mainly governed by a longitudinal localized surface plasmon resonance (LSPR) which arises due to the helical anisotropy of the system. Up to now, experimental studies have predominantly addressed the far-field response, despite the fact that the LSPR being of broad interest for converting incoming light into strongly enhanced (chiral) optical near-fields. Here, we demonstrate the control and spatial reproducibility of the plasmon-induced electromagnetic near-field around metallic nanohelices via surface-enhanced Raman scattering...
May 21, 2018: Nanotechnology
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