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Chao Liang, Kaiyue Wang, Bin Xu, Zhi Yu
BACKGROUND: Electroacupuncture (EA) at acupoint ST 37 (Shangjuxu) has been used to alleviate gastrointestinal symptoms and improve gastrointestinal motility. However, the mechanisms by which EA affects the enteric nervous system (ENS) have scarcely been investigated. In this study, we investigated whether EA could improve ENS function. METHODS: A constipation model was established by gastric instillation of ice-cold saline daily for 14 days. The constipated mice were divided into two groups: the model group, which was not treated, and the EA group, which received EA at ST 37 at a frequency of 2-15 HZ and an amplitude of 1 mA for 15 min a day for 3 days...
October 18, 2016: BMC Complementary and Alternative Medicine
Naho Fujiwara, Katsumi Miyahara, Nana Nakazawa-Tanaka, Chihiro Akazawa, Atsuyuki Yamataka
PURPOSE: Hirschsprung's disease (HD) is caused by a failure of enteric neural crest-derived cells (ENCC) to colonize the bowel, resulting in an absence of the enteric nervous system (ENS). Previously, we developed a Sox10 transgenic version of the Endothelin receptor-B (Ednrb) mouse to visualize ENCC with the green fluorescent protein, Venus. The aim of this study was to isolate Sox10-Venus(+) cells, which are differentiated neurons and glial cells in the ENS, and analyze these cells using Sox10-Venus mice gut...
September 23, 2016: Pediatric Surgery International
A Nishimoto-Kakiuchi, S Netsu, S Matsuo, S Hayashi, T Ito, S Okabayashi, L Yasmin, K Yuzawa, O Kondoh, A Kato, M Suzuki, R Konno, T Sankai
STUDY QUESTION: What are the characteristics of spontaneous endometriosis in cynomolgus monkeys? SUMMARY ANSWER: Spontaneous endometriosis in cynomolgus monkeys exhibited similar characteristics to the human disease. WHAT IS KNOWN ALREADY: One previous report described the prevalence and the basic histopathology of spontaneous endometriosis in cynomolgus monkeys. STUDY DESIGN, SIZE, DURATION: Endometriotic lesions that had been histologically confirmed in 8 female cynomolgus monkeys between 5 and 21 years old were subjected to study...
October 2016: Human Reproduction
Agung Dewanto, Jozsef Dudas, Rudolf Glueckert, Sylvia Mechsner, Anneliese Schrott-Fischer, Ludwig Wildt, Beata Seeber
BACKGROUND: The roles of the neurotrophins NGF (Neurotrophic growth factor) and BDNF (brain-derived neurotrophic factor) in neuronal growth and development are already known. Meanwhile, the neurotrophin receptors TrkA (tropomyosin related kinase A), TrkB, and p75 are important for determining the fate of cells. In endometriosis, this complex system has not been fully elucidated yet. The aim of this study was to evaluate the expression and location of these neurotrophins and their receptors in peritoneal (PE) and deep infiltrating endometriotic (DIE) tissues and to measure and compare the density of nerve fibers in the disease subtypes...
2016: Reproductive Biology and Endocrinology: RB&E
Meng Jiang, Kang Li, Shuai Li, Li Yang, Dehua Yang, Xi Zhang, Mijing Fang, Guoqing Cao, Yong Wang, Weibin Chen, Shaotao Tang
Evaluation of rectal suction biopsies for the ganglion cells and neural hypertrophy is the basic modality for the diagnosis of Hirschsprung's disease (HD). However, the traditional hematoxylin and eosin staining coupled with acetylcholinesterase histochemistry remain challenging, especially in newborns. Thus we conducted a prospective study to evaluate the usefulness of calretinin combined with S100 and protein gene product 9.5 (PGP9.5) immunostaining of rectal suction biopsies for the diagnosis of HD. A total of 195 patients were enrolled in our study...
2016: American Journal of Translational Research
Päivi Tommola, Leila Unkila-Kallio, Anders Paetau, Seppo Meri, Eija Kalso, Jorma Paavonen
BACKGROUND: Provoked vestibulodynia manifests as allodynia of the vulvar vestibular mucosa. The exact mechanisms that result in altered pain sensation are unknown. Recently, we demonstrated the presence of secondary lymphoid tissue, which is the vestibule-associated lymphoid tissue in the vestibular mucosa, and showed that this tissue becomes activated in provoked vestibulodynia. OBJECTIVE: The purpose of this study was to examine whether expression of intraepithelial nerve fibers and nerve growth factor are related to immune activation in provoked vestibulodynia...
July 25, 2016: American Journal of Obstetrics and Gynecology
Sigrid Regauer, Marianne Gamper, Mathias K Fehr, Volker Viereck
PURPOSE: 'Pain' is the key symptom that distinguishes bladder pain syndrome/interstitial cystitis from overactive bladder syndrome, but overlap occurs. To find a discriminating marker for these bladder diseases, we examined sensory hyperinnervation and neurotrophin receptor expression in bladder biopsies, and nerve growth factor levels in urine. MATERIALS AND METHODS: Bladder biopsies from patients with bladder pain syndrome/interstitial cystitis with (n=12) and without (n=19) Hunner lesions, overactive bladder syndrome (n=13), and healthy controls (n=12) were analyzed by immunohistochemistry with antibodies to nerve cell marker PGP9...
July 1, 2016: Journal of Urology
U Anand, M Sinisi, M Fox, A MacQuillan, T Quick, Y Korchev, C Bountra, T McCarthy, P Anand
BACKGROUND: Mycolactone is a polyketide toxin secreted by the mycobacterium Mycobacterium ulcerans, responsible for the extensive hypoalgesic skin lesions characteristic of patients with Buruli ulcer. A recent pre-clinical study proposed that mycolactone may produce analgesia via activation of the angiotensin II type 2 receptor (AT2R). In contrast, AT2R antagonist EMA401 has shown analgesic efficacy in animal models and clinical trials for neuropathic pain. We therefore investigated the morphological and functional effects of mycolactone in cultured human and rat dorsal root ganglia (DRG) neurons and the role of AT2R using EMA401...
2016: Molecular Pain
S Escada-Rebelo, A F Silva, S Amaral, R S Tavares, C Paiva, S Schlatt, J Ramalho-Santos, P C Mota
Spermatogonial stem cells are being exploited in many species as a tool to recover fertility, but may also be used to manipulate the genetic pool. Whatever the purpose, these cells must be fully characterized and easily identifiable, and our goal was to improve this procedure in the domestic cat, used as an animal model for endangered felid species and for some human diseases/physiological processes. We have therefore screened several markers that might be used to distinguish and study the undifferentiated spermatogonia population in situ and in vitro via immunohistochemistry applied to tissue sections and whole mounts of the domestic cat seminiferous tubules...
June 17, 2016: Andrology
Carlos De la Rosa-Prieto, Daniel Saiz-Sanchez, Isabel Ubeda-Banon, Alicia Flores-Cuadrado, Alino Martinez-Marcos
Alzheimer's disease (AD) is the most prevalent neurodegenerative disease, mostly idiopathic and with palliative treatment. Neuropathologically, it is characterized by intracellular neurofibrillary tangles of tau protein and extracellular plaques of amyloid β peptides. The relationship between AD and neurogenesis is unknown, but two facts are particularly relevant. First, early aggregation sites of both proteinopathies include the hippocampal formation and the olfactory bulb (OB), which have been correlated to memory and olfactory deficits, respectively...
2016: Frontiers in Neuroscience
E A Kogan, A S Ovakimyan, N B Paramonova, N M Faizullina, I F Kazachenko, L V Adamyan
UNLABELLED: Endometriosis (EM) is morphologically characterized by the development of extrauterine endometrioid heterotopies, the major clinical symptoms of which is chronic pelvic pain, which is a serious problem not only in modern gynecology, but also in public health as a whole. AIM: to investigate neurogenic markers in the foci of EM of various sites and histological structure in women with and without pain syndrome. MATERIAL AND METHODS: The investigation was performed using the operative material (resected segments of the intestine, bladder, rectovaginal septum, and small pelvic peritoneum) obtained from 52 women with an intraoperative and morphologically verified diagnosis of EM and (Group 1) and without (Group 2) pain syndrome...
May 2016: Arkhiv Patologii
Xiao Yan Yu, Li Zhang, Xiu Ying Yang, Xiao Ting Li, Guan Hua Du
OBJECTIVE: This study aimed to detect the effect of a new herbal extract salvianolic acid A (SalA) on gastrointestinal complications in diabetic rats. METHODS: Altogether 80 rats were divided randomly into five groups, including normal control (NC) group, high-fat (HF) diet group, diabetes mellitus (DM) control group, and DM treated with SalA (0.1 mg/kg and 0.3 mg/kg) groups, respectively. DM was induced by feeding the rats with HF diet and the administration of streptozotocin (30 mg/kg)...
July 2016: Journal of Digestive Diseases
S Mizukami, T Murakami, T Tanaka, N Machida, K Nomura, T Yoshida, M Shibutani
The present study has characterized the germ cell component of canine testicular mixed germ cell-sex cord stromal tumours (MGSCTs) by examining the histological nature and histochemical and immunohistochemical features using gonocytic and spermatogonial cellular markers, c-Kit, placental alkaline phosphatase (PLAP), protein gene product 9.5 (PGP9.5), Sal-like protein 4 (SALL4), and the periodic acid-Schiff (PAS) reaction. Histologically, all 45 examples of MGSCTs were classified as spermatocytic seminomas (SSs) and Sertoli cell tumours in combination...
July 2016: Journal of Comparative Pathology
Nathalie Van Acker, Michael Ragé, Ellen Sluydts, Michiel W M Knaapen, Martine De Bie, Maarten Timmers, Erik Fransen, Carla Duymelinck, Stefanie De Schepper, Praveen Anand, Theo Meert, Léon Plaghki, Patrick Cras
BACKGROUND: In this study we explored the possibility of automating the PGP9.5 immunofluorescence staining assay for the diagnosis of small fiber neuropathy using skin punch biopsies. The laboratory developed test (LDT) was subjected to a validation strategy as required by good laboratory practice guidelines and compared to the well-established gold standard method approved by the European Federation of Neurological Societies (EFNS). To facilitate automation, the use of thinner sections...
2016: BMC Research Notes
Cordula Netzer, Karin Urech, Thomas Hügle, Robyn Melanie Benz, Jeroen Geurts, Stefan Schären
Facet joint osteoarthritis may be a cause of low back pain in degenerative spine diseases including lumbar spinal stenosis. Subchondral bone is regarded as a potential therapeutic target for osteoarthritis treatment. The goal of this study was to characterize subchondral bone histopathology in osteoarthritic facet joints from lumbar spinal stenosis patients. Fifteen patients with degenerative spinal stenosis scheduled for transforaminal lumbar interbody fusion surgery were recruited for this study. Osteoarthritis severity was graded on T1- and T2-weighted MRI images using Weishaupt scoring system...
August 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Pan Luo, Cheng Chen, Yun Lu, TianLi Fu, Qing Lu, Xulin Xu, Changjun Li, Zhi He, Lianjun Guo
Chronic cerebral hypoperfusion (CCH) causes memory deficits and increases the risk of vascular dementia (VD) through several biologically plausible pathways. However, whether CCH causes prefrontal cortex (PFC)-dependent spatial working memory impairments and Baclofen, a GABAB receptor agonist, could ameliorate the impairments is still not clear especially the mechanisms underlying the process. In this study, rats were subjected to permanent bilateral occlusion of the common carotid arteries (two-vessel occlusion, 2VO) to induce CCH...
July 15, 2016: Behavioural Brain Research
Niraj Kumari, Nandita Chaudhary, Roma Pradhan, Amit Agarwal, Narendra Krishnani
Parathyroid carcinoma (PC) is a rare neoplasm accounting for 0.5-6 % of primary hyperparathyroidism. Histological criteria are currently considered as established means to diagnose malignancy in parathyroid neoplasms; however, it does not accurately predict the risk of aggressive behaviour of PC. Immunohistochemical (IHC) markers have been used in the literature with variable results. This work was planned to study whether IHC markers would have any added advantage over histology in predicting outcome in parathyroid neoplasms...
June 2016: Endocrine Pathology
Dandan He, Adriana Manzoni, Diego Florentin, William Fisher, Yi Ding, MinJae Lee, Gustavo Ayala
Pancreatic cancer (PaCA) is a deadly disease with few systemic therapeutic options. The head of the pancreas is the most innervated part and most common location of cancer. However, little is known about the contribution of the nerve-cancer interaction to facilitate pancreatic progression. To quantify PaCA axonogenesis, we used a 3-dimensional in vitro neurogenesis model. In addition, neurogenesis in human PaCA was analyzed using PGP9.5 immunohistochemistry, deconvolution imaging, and image segmentation and analysis...
June 2016: Human Pathology
Chao Liang, Kai-Yue Wang, Zhi Yu, Bin Xu
AIM: To establish a novel mouse constipation model. METHODS: Animals were randomly divided into three groups, and intragastrically administered 0-4 °C saline (ice-cold group) or 15-20 °C saline (saline control group) daily for 14 d, or were left untreated (blank control group). Stools were collected 3-24 h after treatment to record the wet and dry weights and the stool form. Intestinal propulsion experiments were carried out and defecation time was measured for six days continuously after suspending treatments...
March 7, 2016: World Journal of Gastroenterology: WJG
Wanyou Feng, Shibei Chen, Dagiang Do, Qinyou Liu, Yanfei Deng, Xiaocan Lei, Chan Luo, Ben Huang, Deshun Shi
Isolation and culture of spermatogonial stem cells (SSCs) are attractive for production of genetic modified offspring. In the present study, buffalo spermatogonial stem-like cells were isolated, cultured and expression pattern of different germ cell marker genes were determined. To recover spermatogonia, testes from age 3 to 7 months of buffalo were decapsulated, and seminiferous tubules were enzymatically dissociated. Two types of cells, immature sertoli cell and type A spermatogonia were observed in buffalo testes in this stage...
October 2016: Asian-Australasian Journal of Animal Sciences
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