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https://www.readbyqxmd.com/read/28465081/characterization-of-male-germ-cell-markers-in-canine-testis
#1
Won-Young Lee, Ran Lee, Hyun-Jung Park, Jeong Tae Do, Chankyu Park, Jin-Hoi Kim, Hyunjhung Jhun, Ji-Heon Lee, Taiyoung Hur, Hyuk Song
Spermatogenesis begins at puberty and continues throughout a male's life. This process is initiated and maintained by spermatogonial stem cells in the seminiferous tubules, and these cells produce haploid spermatozoa. Markers of male germ cells have been fully identified in rodents, including mice and rats, but not in canines. To characterize the canine male germ cells, histological and immunohistochemical analyses were performed, using prepubertal (1-3-month-old), early pubertal (4-month-old), and postpubertal (7-month-old) dog testes...
January 12, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28401161/estrogen-replacement-regulates-vaginal-innervations-in-ovariectomized-adult-virgin-rats-a-histological-study
#2
Ting Li, Yuanyuan Ma, Hong Zhang, Ping Yan, Lili Huo, Yongyan Hu, Xi Chen, Ting Li, Miao Zhang, Zhaohui Liu
Background. Our previous Gräfenberg spot findings confirmed that the distal-third areas of the anterior vaginal wall bore a significantly greater number of nerves and sexual hormone may have certain degree of influence on these significant differences. However, the role of estrogen in vaginal innervations remains controversial. Methods. To investigate whether hormonal-neural interactions occur in the vagina, sixty rats were randomly divided into six groups: Sham-operated, ovariectomy, and 4 treatment groups...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28371926/sensory-innervation-of-perivascular-adipose-tissue-a-crucial-role-in-artery-vasodilatation-and-leptin-release
#3
Hamidah Abu Bakar, William Robert Dunn, Craig Daly, Vera Ralevic
Aims: Electrical field stimulation (EFS) elicits robust sensory neurogenic relaxation responses in the rat isolated mesenteric arterial bed but these responses are absent or difficult to demonstrate in isolated arteries. We believe that this mismatch is due to the absence of perivascular adipose tissue (PVAT) as it is conventionally removed in studies on isolated vessels. We aimed to determine whether sensory nerves are expressed in PVAT, their physiological roles and their possible interactions with PVAT-derived adipokines...
March 23, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28270361/morphological-and-immunohistochemical-comparison-of-intrapancreatic-nerves-between-chronic-pancreatitis-and-type-1-autoimmune-pancreatitis
#4
Kota Kato, Tsukasa Ikeura, Masato Yanagawa, Takashi Tomiyama, Toshiro Fukui, Kazushige Uchida, Makoto Takaoka, Akiyoshi Nishio, Yoshiko Uemura, Sohei Satoi, Hisao Yamada, Kazuichi Okazaki
OBJECTIVES: The abdominal pain associated with chronic pancreatitis (CP) may be related to the increased number and size of intrapancreatic nerves. On the other hand, patients with type 1 autoimmune pancreatitis (AIP) rarely suffer from the pain syndrome, and there are no previous studies concerning the histopathological findings of intrapancreatic nerves in patients with type 1 AIP. The current study is aimed at investigating the differences in the histopathological and immunohistochemical findings of intrapancreatic nerves in patients with CP and type 1 AIP...
February 20, 2017: Pancreatology: Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
https://www.readbyqxmd.com/read/28152579/5htr3a-driven-gfp-labels-immature-olfactory-sensory-neurons
#5
Thomas E Finger, Dianna L Bartel, Nicole Shultz, Noah B Goodson, Charles A Greer
The ionotropic serotonin receptor, 5-HT3 , is expressed by many developing neurons within the central nervous system. Since the olfactory epithelium continues to generate new olfactory sensory neurons (OSNs) throughout life, we investigated the possibility that 5-HT3 is expressed in the adult epithelium. Using a transgenic mouse in which the promoter for the 5-HT3a subunit drives expression of green fluorescent protein (GFP), we assessed the expression of this marker in the olfactory epithelium of adult mice...
February 2, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/28090391/neurotoxin-induced-catecholaminergic-loss-in-the-colonic-myenteric-plexus-of-rhesus-monkeys
#6
Jeanette M Shultz, Henry Resnikoff, Viktorya Bondarenko, Valerie Joers, Andres Mejia, Heather Simmons, Marina E Emborg
OBJECTIVE: Constipation is a common non-motor symptom of Parkinson's disease (PD). Although pathology of the enteric nervous system (ENS) has been associated with constipation in PD, the contribution of catecholaminergic neurodegeneration to this symptom is currently debated. The goal of this study was to assess the effects of the neurotoxin 6-hydroxydopamine (6-OHDA) on the colonic myenteric plexus and shed light on the role of catecholaminergic innervation in gastrointestinal (GI) function...
November 2016: Journal of Alzheimer's Disease and Parkinsonism
https://www.readbyqxmd.com/read/28066953/diabetic-and-idiopathic-gastroparesis-is-associated-with-loss-of-cd206-positive-macrophages-in-the-gastric-antrum
#7
M Grover, C E Bernard, P J Pasricha, H P Parkman, S J Gibbons, J Tonascia, K L Koch, R W McCallum, I Sarosiek, W L Hasler, L A B Nguyen, T L Abell, W J Snape, M L Kendrick, T A Kellogg, T J McKenzie, F A Hamilton, G Farrugia
BACKGROUND: Animal studies have increasingly highlighted the role of macrophages in the development of delayed gastric emptying. However, their role in the pathophysiology of human gastroparesis is unclear. Our aim was to determine changes in macrophages and other cell types in the gastric antrum muscularis propria of patients with diabetic and idiopathic gastroparesis. METHODS: Full thickness gastric antrum biopsies were obtained from patients enrolled in the Gastroparesis Clinical Research Consortium (11 diabetic, 6 idiopathic) and 5 controls...
June 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/28053620/establishment-of-a-surgically-induced-cryptorchidism-canine-recipient-model-for-spermatogonial-stem-cell-transplantation
#8
Won-Young Lee, Ran Lee, Hyuk Song, Tai-Young Hur, Seunghoon Lee, Jiyun Ahn, Hyunjhung Jhun
Transplantation of spermatogonial stem cells (SSCs) in experimental animal models has been used to study germ line stem cell biology and to produce transgenic animals. The species-specific recipient model preparation is important for the characterization of SSCs and the production of offspring. Here, we investigated the effects of surgically induced cryptorchidism in dog as a new recipient model for spermatogonial stem cell transplantation. Artificially unilateral or bilateral cryptorchidism was induced in ten mature male dogs by surgically returning the testis and epididymis to the abdominal cavity...
December 2016: Laboratory Animal Research
https://www.readbyqxmd.com/read/28028361/characterization-of-smooth-muscle-enteric-nerve-interstitial-cells-of-cajal-and-fibroblast-like-cells-in-the-gastric-musculature-of-patients-with-diabetes-mellitus
#9
Kyung Sik Park, Kwang Bum Cho, Il Seon Hwang, Jae Hyung Park, Byung Ik Jang, Kyeong Ok Kim, Sung Woo Jeon, Eun Soo Kim, Chang Sik Park, Joong Goo Kwon
AIM: To investigate histologic abnormalities in the gastric smooth muscle of patients with diabetes mellitus (DM). METHODS: Full-thickness gastric specimens were obtained from patients undergoing surgery for gastric cancer. H&E stain and Masson's Trichrome stain were performed to assess the degree of fibrosis. Immunohistochemical staining using various antibodies was also performed [antibodies against protein gene product 9.5 (PGP9.5), neuronal nitric oxide synthase (nNOS), vasoactive intestinal peptide (VIP), neurokinin-1 (NK1) receptor, c-Kit, and platelet-derived growth factor receptor-alpha, (PDGFRα)]...
December 14, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28013333/semaphorin-3a-expression-following-intestinal-ischemia-reperfusion-injury-in-sox10-venus-mice
#10
Masahiro Takeda, Katsumi Miyahara, Manabu Okawada, Chihiro Akazawa, Geoffrey J Lane, Atsuyuki Yamataka
PURPOSE: Semaphorin 3A (Sema3A) is a protein secreted during development of the nervous system that plays an important role in neuronal pathophysiology. However, there is no known correlation between Sema3A and intestinal ischemia/reperfusion (I/R) injury. We assessed Sema3A expression and distribution in relation to enteric nervous system (ENS) damage seen after intestinal I/R injury in Sox10-Venus mice. METHODS: Intestinal I/R injury was induced by vascular occlusion for 3 h...
March 2017: Pediatric Surgery International
https://www.readbyqxmd.com/read/28002923/tendinopathic-supraspinatus-tenocytes-may-have-a-neuroendocrine-like-function-secreting-cgrp-sp-and-vegf-a-pilot-immunohistochemistry-study
#11
R Sahemey, P Malliaras, J S Lewis, G J Michael, P Shortland, D Morrissey, N Maffulli
We wanted to observe and compare the appearance of neurovascular tissue from tendon ex vivo, in patients with and without painful rotator cuff tendinopathy. Supraspinatus tendons were biopsied from 5 participants with painful tendinopathy and normal tendon from a young male. Slides were stained with haematoxylin and eosin and toluidine blue for histological assessment. Immunohistochemical markers for general nerves (protein gene-product 9.5 and synaptophysin), sensory nerves (calcitonin gene-related peptide; substance-P) and vascularisation (vascular endothelial growth factor) were used...
October 2016: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/27914685/protein-gene-product-9-5-pgp9-5-expression-in-benign-cutaneous-mesenchymal-histiocytic-and-melanocytic-lesions-comparison-with-cellular-neurothekeoma
#12
COMPARATIVE STUDY
Grace Y Wang, Rosalynn M Nazarian, Lili Zhao, Alexandra C Hristov, Rajiv M Patel, Douglas R Fullen, May P Chan
Cellular neurothekeoma (CNTK) frequently enters the differential diagnosis of a benign dermal cellular proliferation. Diagnosis often relies on immunohistochemistry including the use of protein gene product 9.5 (PGP9.5). A previous study demonstrated PGP9.5 expression across a wide variety of soft tissue neoplasms. We explored the utility of this antibody in distinguishing CNTK from other benign dermal-based lesions. A cohort of CNTK (n=7) and benign cutaneous lesions of neural (n=28), fibrohistiocytic (n=23), fibroblastic (n=25), histiocytic (n=18), myofibroblastic (n=7), smooth muscle (n=14), and melanocytic (n=12) differentiations were immunostained with PGP9...
January 2017: Pathology
https://www.readbyqxmd.com/read/27865118/dienogest-reduces-proliferation-ngf-expression-and-nerve-fiber-density-in-human-adenomyosis
#13
Arisa Takeuchi, Kaori Koga, Mariko Miyashita, Tomoko Makabe, Fusako Sue, Miyuki Harada, Tetsuya Hirata, Yasushi Hirota, Tomoyuki Fujii, Yutaka Osuga
OBJECTIVES: To evaluate the in vivo effect of dienogest on proliferation, apoptosis, aromatase expression, vascular density, nerve growth factor (NGF) expression and nerve fiber density in human adenomyosis tissue. STUDY DESIGN: Twelve women who underwent hysterectomy for adenomyosis were enrolled. Six patients received dienogest treatment prior to hysterectomy (dienogest group), and age-matched six patients who had not received any hormonal treatment for ≥3 months before surgery (control group)...
December 2016: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/27856290/enhanced-neurogenic-biomarker-expression-and-reinnervation-in-human%C3%A2-acute-skin-wounds-treated-by-electrical-stimulation
#14
Anil Sebastian, Susan W Volk, Poonam Halai, James Colthurst, Ralf Paus, Ardeshir Bayat
Electrical stimulation (ES) is known to promote cutaneous healing; however, its ability to regulate reinnervation remains unclear. First, we show that ES treatment of human acute cutaneous wounds (n = 40) increased reinnervation. Next, to define neurophysiologic mechanisms through which ES affects repair, microarray analysis of wound biopsy samples was performed on days 3, 7, 10, and 14 after wounding. This identified neural differentiation biomarkers TUBB3 (melanocyte development and neuronal marker) and its upstream molecule FIG4 (phosphatidylinositol (3,5)-bisphosphate 5-phosphatase) as significantly up-regulated after ES treatment...
March 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/27776012/peripheral-neuropathic-changes-in-pachyonychia-congenita
#15
Baohan Pan, Kelly Byrnes, Mary Schwartz, C David Hansen, Claudia M Campbell, Malvina Krupiczojc, Michael J Caterina, Michael Polydefkis
We compared patterns of intraepidermal nerve fibers and mechanoreceptors from affected and unaffected plantar skin from patients with pachyonychia congenita (PC) and control subjects. Plantar biopsies from 10 genetically confirmed patients with PC (with a mutation in KRT6A) were performed at the ball of the foot (affected skin) and the arch (unaffected) and were compared to biopsies from corresponding locations in 10 control subjects. Tissue was processed to visualize intraepidermal nerve fibers (IENF) (PGP9...
October 20, 2016: Pain
https://www.readbyqxmd.com/read/27756367/electroacupuncture-at-acupoint-st-37-shangjuxu-improves-function-of-the-enteric-nervous-system-in-a-novel-mouse-constipation-model
#16
Chao Liang, Kaiyue Wang, Bin Xu, Zhi Yu
BACKGROUND: Electroacupuncture (EA) at acupoint ST 37 (Shangjuxu) has been used to alleviate gastrointestinal symptoms and improve gastrointestinal motility. However, the mechanisms by which EA affects the enteric nervous system (ENS) have scarcely been investigated. In this study, we investigated whether EA could improve ENS function. METHODS: A constipation model was established by gastric instillation of ice-cold saline daily for 14 days. The constipated mice were divided into two groups: the model group, which was not treated, and the EA group, which received EA at ST 37 at a frequency of 2-15 HZ and an amplitude of 1 mA for 15 min a day for 3 days...
October 18, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27663687/altered-differentiation-of-enteric-neural-crest-derived-cells-from-endothelin-receptor-b-null-mouse-model-of-hirschsprung-s-disease
#17
Naho Fujiwara, Katsumi Miyahara, Nana Nakazawa-Tanaka, Chihiro Akazawa, Atsuyuki Yamataka
PURPOSE: Hirschsprung's disease (HD) is caused by a failure of enteric neural crest-derived cells (ENCC) to colonize the bowel, resulting in an absence of the enteric nervous system (ENS). Previously, we developed a Sox10 transgenic version of the Endothelin receptor-B (Ednrb) mouse to visualize ENCC with the green fluorescent protein, Venus. The aim of this study was to isolate Sox10-Venus(+) cells, which are differentiated neurons and glial cells in the ENS, and analyze these cells using Sox10-Venus mice gut...
December 2016: Pediatric Surgery International
https://www.readbyqxmd.com/read/27591226/characteristics-of-histologically-confirmed-endometriosis-in-cynomolgus-monkeys
#18
A Nishimoto-Kakiuchi, S Netsu, S Matsuo, S Hayashi, T Ito, S Okabayashi, L Yasmin, K Yuzawa, O Kondoh, A Kato, M Suzuki, R Konno, T Sankai
STUDY QUESTION: What are the characteristics of spontaneous endometriosis in cynomolgus monkeys? SUMMARY ANSWER: Spontaneous endometriosis in cynomolgus monkeys exhibited similar characteristics to the human disease. WHAT IS KNOWN ALREADY: One previous report described the prevalence and the basic histopathology of spontaneous endometriosis in cynomolgus monkeys. STUDY DESIGN, SIZE, DURATION: Endometriotic lesions that had been histologically confirmed in 8 female cynomolgus monkeys between 5 and 21 years old were subjected to study...
October 2016: Human Reproduction
https://www.readbyqxmd.com/read/27519317/localization-of-trkb-and-p75-receptors-in-peritoneal-and-deep-infiltrating-endometriosis-an-immunohistochemical-study
#19
Agung Dewanto, Jozsef Dudas, Rudolf Glueckert, Sylvia Mechsner, Anneliese Schrott-Fischer, Ludwig Wildt, Beata Seeber
BACKGROUND: The roles of the neurotrophins NGF (Neurotrophic growth factor) and BDNF (brain-derived neurotrophic factor) in neuronal growth and development are already known. Meanwhile, the neurotrophin receptors TrkA (tropomyosin related kinase A), TrkB, and p75 are important for determining the fate of cells. In endometriosis, this complex system has not been fully elucidated yet. The aim of this study was to evaluate the expression and location of these neurotrophins and their receptors in peritoneal (PE) and deep infiltrating endometriotic (DIE) tissues and to measure and compare the density of nerve fibers in the disease subtypes...
August 12, 2016: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/27508037/calretinin-s100-and-protein-gene-product-9-5-immunostaining-of-rectal-suction-biopsies-in-the-diagnosis-of-hirschsprung-disease
#20
Meng Jiang, Kang Li, Shuai Li, Li Yang, Dehua Yang, Xi Zhang, Mijing Fang, Guoqing Cao, Yong Wang, Weibin Chen, Shaotao Tang
Evaluation of rectal suction biopsies for the ganglion cells and neural hypertrophy is the basic modality for the diagnosis of Hirschsprung's disease (HD). However, the traditional hematoxylin and eosin staining coupled with acetylcholinesterase histochemistry remain challenging, especially in newborns. Thus we conducted a prospective study to evaluate the usefulness of calretinin combined with S100 and protein gene product 9.5 (PGP9.5) immunostaining of rectal suction biopsies for the diagnosis of HD. A total of 195 patients were enrolled in our study...
2016: American Journal of Translational Research
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