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https://www.readbyqxmd.com/read/29234985/ponticoccus-alexandrii-sp-nov-a-novel-bacterium-isolated-from-the-marine-toxigenic-dinoflagellate-alexandrium-minutum
#1
Qiao Yang, Xiaoling Zhang, Lingzhi Li, Ruonan Zhang, Lijuan Feng, Jun Mu
During an investigation of the biodiversity of the cultivable bacterial community associated with paralytic shellfish poisoning toxin-producing marine dinoflagellate, Alexandrium minutum a novel algal-associated bacterium, designated strain AT2-AT was isolated. 16S rRNA gene sequence similarity analysis showed that the strain is a member of the genus Ponticoccus, with high sequence similarity to Ponticoccus litoralis DSM 18986T (97.9%) and Ponticoccus lacteus JCM 30379T (96.0%). However, based on the data obtained for the physiological and biochemical characteristics, and low level of DNA-DNA relatedness analysis, the strain could be genotypically and phenotypically differentiated from two type strains of the genus Ponticoccus...
December 12, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29234119/non-malignant-epithelial-cells-preferentially-proliferate-from-nasopharyngeal-carcinoma-biopsy-cultured-under-conditionally-reprogrammed-conditions
#2
Fenggang Yu, Yanan Lu, Lin Tao, Yan-Yi Jiang, De-Chen Lin, Lingzhi Wang, Fredrik Petersson, Hironori Yoshiyama, Phillip H Koeffler, Boon-Cher Goh, Kwok Seng Loh
Nasopharyngeal carcinoma (NPC) is an invasive cancer with particularly high incidence in Southern China and Southeast Asia. The study of NPC is greatly hampered by the lack of reliable cell lines due to the loss of EBV genome and HeLa cell contamination. Conditional reprogramming (CR) cell culture technique has been reported for rapid and efficient establishment of patient-derived normal and tumor cell cultures. The purpose of this study was to assess this method to culture NPC patient-derived primary tumor cells...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29199580/what-is-ganoderma-lucidum-in-the-molecular-era
#3
Viktor Papp, Bálint Dima, Solomon P Wasser
The scientific name Ganoderma lucidum has long been used in the global medicinal mushroom market, and many publications have proven its pharmacological properties and chemical components. Although several Ganoderma species have been described worldwide as having morphology considerably similar to that of G. lucidum s. str., this binomial is still the most frequently used in pharmacological studies. However, the use of particularly divergent nomenclature has caused major difficulties in the evaluation of the results of pharmacological studies...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/29199568/lingzhi-or-reishi-medicinal-mushroom-ganoderma-lucidum-agaricomycetes-inhibits-candida-biofilms-a-metabolomic-approach
#4
Anuja Bhardwaj, Payal Gupta, Navin Kumar, Jigni Mishra, Ajai Kumar, Rajput Rakhee, Kshipra Misra
This article presents a comparative gas chromatography (GC)-mass spectrometry (MS)-based metabolomic analysis of mycelia and fruiting bodies of the medicinal mushroom Ganoderma lucidum. Three aqueous extracts-mycelia, fruiting bodies, and a mixture of them-and their sequential fractions (methanolic and ethyl acetate), prepared using an accelerated solvent extractor, were characterized by GC-MS to determine volatile organic compounds and by high-performance thin-layer chromatography to quantify ascorbic acid, a potent antioxidant...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/29199554/antioxidative-and-inhibitory-effects-of-the-fruiting-body-of-black-lingzhi-mushroom-amauroderma-rugosum-agaricomycetes-on-ldl-oxidation-and-hmg-coa-reductase-activity
#5
Chan Kam Seng, Noorlidah Abdullah, Norhaniza Aminudin
Amauroderma rugosum fruiting bodies possess excellent cardiovascular benefits, including antioxidative, antihyperlipidemic, antihypertensive, antiinflammatory, anti-platelet aggregation, and antithrombotic effects. In this article, we describe our investigations of the in vitro antioxidant activity and in vitro antiatherosclerotic potential through inhibitory effects on low-density lipoprotein (LDL), LDL peroxidation, and 3-hydroxy3-methylglutaryl-coenzyme A (HMG-CoA) reductase catalytic activity using various fruiting body extracts partitioned with an organic solvent...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/29184907/comparative-assessment-of-anti-hla-antibodies-using-two-commercially-available-luminex-based-assays
#6
Kevin J Clerkin, Sarah B See, Maryjane A Farr, Susan W Restaino, Geo Serban, Farhana Latif, Lingzhi Li, Paolo C Colombo, George Vlad, Bryan Ray, Elena R Vasilescu, Emmanuel Zorn
Background: Allospecific anti-HLA antibodies (Abs) are associated with rejection of solid organ grafts. The 2 main kits to detect anti-HLA Ab in patient serum are commercialized by Immucor and One Lambda/ThermoFisher. We sought to compare the performance of both platforms. Methods: Background-adjusted mean fluorescence intensity (MFI) values were used from both platforms to compare sera collected from 125 pretransplant and posttransplant heart and lung transplant recipients...
November 2017: Transplantation Direct
https://www.readbyqxmd.com/read/29180767/frequency-and-reactivity-of-antigen-specific-t-cells-were-concurrently-measured-through-the-combination-of-artificial-antigen-presenting-cell-macs-and-elispot
#7
Chuanlai Shen, Tao Xu, You Wu, Xiaoe Li, Lingzhi Xia, Wei Wang, Khawar Ali Shahzad, Lei Zhang, Xin Wan, Jie Qiu
Conventional peptide-major histocompatibility complex (pMHC) multimer staining, intracellular cytokine staining, and enzyme-linked immunospot (ELISPOT) assay cannot concurrently determine the frequency and reactivity of antigen-specific T cells (AST) in a single assay. In this report, pMHC multimer, magnetic-activated cell sorting (MACS), and ELISPOT techniques have been integrated into a micro well by coupling pMHC multimers onto cell-sized magnetic beads to characterize AST cell populations in a 96-well microplate which pre-coated with cytokine-capture antibodies...
November 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29179207/reduction-of-nanog-mediates-the-inhibitory-effect-of-aspirin-on-tumor-growth-and-stemness-in-colorectal-cancer
#8
Hefei Wang, Bing Liu, Jing Wang, Jinglin Li, Ying Gong, Sisi Li, Chunli Wang, Bai Cui, Xiaoyuan Xue, Mengying Yang, Wenjun Fan, Zhijie Kang, Muhammad Kamran, Jie Xu, Pengfei Tian, Yuanyuan Luo, Zhijie Hou, Lin Dong, Yanling Ren, Man Li, Qingping Wen, Wei Cheng, Lingzhi Xu, Ling Wang, Quentin Liu
BACKGROUND/AIMS: Cancer stem cells (CSCs) are considered to be responsible for tumor relapse and metastasis, which serve as a potential therapeutic target for cancer. Aspirin has been shown to reduce cancer risk and mortality, particularly in colorectal cancer. However, the CSCs-suppressing effect of aspirin and its relevant mechanisms in colorectal cancer remain unclear. METHODS: CCK8 assay was employed to detect the cell viability. Sphere formation assay, colony formation assay, and ALDH1 assay were performed to identify the effects of aspirin on CSC properties...
November 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29162539/modulation-of-diverse-oncogenic-transcription-factors-by-thymoquinone-an-essential-oil-compound-isolated-from-the-seeds-of-nigella-sativa-linn
#9
REVIEW
Muthu K Shanmugam, Frank Arfuso, Alan Prem Kumar, Lingzhi Wang, Boon Cher Goh, Kwang Seok Ahn, Anupam Bishayee, Gautam Sethi
Thymoquinone (TQ), isolated almost fifty years ago, is the main bioactive constituent of black seed essential oil extracted from the seed of Nigella sativa. TQ has been shown to have promising effects against a variety of inflammatory diseases and cancer. Cancer development is a multistep process where normal cells acquire qualities that enable the cells to proliferate continuously and migrate to distant sites in the human body. Drugs that interfere with this process are considered potential anti-cancer therapeutics, which may ultimately result in their clinical usage...
November 18, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29140080/vapor-assisted-solution-approach-for-high-quality-perovskite-ch3nh3pbbr3-thin-films-for-high-performance-green-light-emitting-diodes-applications
#10
Huifang Ji, Zhifeng Shi, Xuguang Sun, Ying Li, Sen Li, Lingzhi Lei, Di Wu, Tingting Xu, Xinjian Li, Guotong Du
Vapor-assisted solution method was developed to prepare the high-quality organic-inorganic halide perovskite CH3NH3PbBr3 (MAPbBr3) thin films. We detailedly investigated the effect of evaporation time and temperature of MABr powder on the microstructure, crystallinity and optical characterizations of MAPbBr3 thin films, and a controllable morphology evolution with varying surface coverage was observed. Temperature-dependent and time-resolved photoluminescence (PL) measurements were carried out to investigate the optical transition mechanisms and carrier recombination dynamics of MAPbBr3 thin films...
November 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29116589/extracellular-vesicles-novel-vehicles-in-herpesvirus-infection
#11
REVIEW
Lingzhi Liu, Quan Zhou, Yan Xie, Lielian Zuo, Fanxiu Zhu, Jianhong Lu
Herpesviruses are remarkable pathogens that have evolved multiple mechanisms to evade host immunity, ensuring their proliferation and egress. Among these mechanisms, herpesviruses utilize elaborate extracellular vesicles, including exosomes, for the intricate interplay between infected host and recipient cells. Herpesviruses incorporate genome expression products and direct cellular products into exosomal cargoes. These components alter the content and function of exosomes released from donor cells, thus affecting the downstream signalings of recipient cells...
October 2017: Virologica Sinica
https://www.readbyqxmd.com/read/29115581/tramadol-attenuates-the-sensitivity-of-glioblastoma-to-temozolomide-through-the-suppression-of-cx43%C3%A2-mediated-gap-junction-intercellular-communication
#12
Lingzhi Wang, Yuexia Peng, Jianxin Peng, Min Shao, Li Ma, Zhuoli Zhu, Guocheng Zhong, Zhengyuan Xia, Huansen Huang
Analgesics and antineoplastic drugs are often used concurrently for cancer patients. Our previous study reported that gap junctions composed of connexin32 (Cx32) was implicated in the effect of analgesics on cisplatin cytotoxicity. However, the effect of analgesic on the most widely expressed connexin (Cx), connexin43 (Cx43), and whether such effect mediates the influence on chemotherapeutic efficiency remain unknown. By manipulation of Cx43 expression or gap junction function, we found that there were gap junction-dependent and independent effect of Cx43 on temozolomide (TMZ) sensitivity in U87 glioblastoma cells...
January 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/29109180/isolation-of-aggressive-behavior-mutants-in-drosophila%C3%A2-using-a-screen-for-wing-damage
#13
Shaun M Davis, Amanda L Thomas, Lingzhi Liu, Ian M Campbell, Herman A Dierick
Aggression is a complex social behavior that is widespread in nature. To date only a limited number of genes that affect aggression have been identified, in large part because the complexity of the phenotype makes screening difficult and time consuming regardless of the species that is studied. We discovered that aggressive group-housed Drosophila melanogaster males inflict damage on each other's wings, and show that wing damage negatively affects their ability to fly and mate. Using this wing-damage phenotype, we screened males from ~1,400 chemically mutagenized strains and found ~40 mutant strains with substantial wing damage...
November 6, 2017: Genetics
https://www.readbyqxmd.com/read/29096260/a-novel-process-for-preparation-of-titanium-dioxide-from-ti-bearing-electric-furnace-slag-nh4hf2-hf-leaching-and-hydrolyzing-process
#14
Fuqiang Zheng, Yufeng Guo, Guanzhou Qiu, Feng Chen, Shuai Wang, Yulei Sui, Tao Jiang, Lingzhi Yang
A novel process to prepare titanium dioxide from Ti-bearing electric furnace slag by NH4HF2-HF leaching and hydrolyzing process has been developed. In this present study, the effects of [NH4(+)]/[F] mXolar ratio, leaching temperature, [F] concentration, liquid/solid mass ratio, leaching time on the Ti extraction, and the phase transformations have been investigated to reveal the leaching mechanism of Ti-bearing electric furnace slag in NH4HF2-HF solution. In the NH4HF2-HF leaching process, the MgTi2O5 and Al2TiO5 are converted to TiF6(2-) and Mg-Al-bearing precipitate...
October 28, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29078264/overview-of-ganoderma-sinense-polysaccharide-an-adjunctive-drug-used-during-concurrent-chemo-radiation-therapy-for-cancer-treatment-in-china
#15
REVIEW
Yifei Jiang, Yajing Chang, Yong Liu, Meng Zhang, Heng Luo, Cui Hao, Pengjiao Zeng, Yue Sun, Hua Wang, Lijuan Zhang
Ganoderma sinense or "Chinese Lingzhi" is a well-known medicinal fungus in China for more than 2000 years. Polysaccharide is the main immunomodulatory and antitumor component in G. sinense. In 2010, G. sinense polysaccharide (GSP) tablet is approved as an adjunctive therapeutic drug in China for treating leukopenia and hematopoietic injury caused by concurrent chemo/radiation therapy during cancer treatment by the State Food and Drug Administration (SFDA). β-glucan, an established immunostimulant, is one of the components in GSP...
October 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29065629/exploring-space-management-goals-in-institutional-care-facilities-in-china
#16
Lingzhi Li, Jingfeng Yuan, Yan Ning, Qiuhu Shao, Jiankun Zhang
Space management has been widely examined in commercial facilities, educational facilities, and hospitals but not in China's institutional care facilities. Poor spatial arrangements, such as wasted space, dysfunctionality, and environment mismanagement, are increasing; in turn, the occupancy rate is decreasing due to residential dissatisfaction. To address these problems, this paper's objective is to explore the space management goals (SMGs) in institutional care facilities in China. Systematic literature analysis was adopted to set SMGs' principles, to identify nine theoretical SMGs, and to develop the conceptual model of SMGs for institutional care facilities...
2017: Journal of Healthcare Engineering
https://www.readbyqxmd.com/read/29050340/nogo-b-promotes-emt-in-lung-fibrosis-via-mmp14-mediates-free-tgf-beta1-formation
#17
Ye Xiong, Jing Zhang, Lingzhi Shi, Yunye Ning, Ying Zhu, Si Chen, Meng Yang, Jingyu Chen, Guo-Wu Zhou, Qiang Li
Idiopathic pulmonary fibrosis (IPF) is a lung disease with an extremely poor prognosis. Epithelial mesenchymal transition (EMT) appearing on the airway epithelial cell plays an essential role in the formation and development of Idiopathic pulmonary fibrosis. In this paper, Bleomycin (BLM)-induced mice model combined with bioinformatics analysis were employed to elucidate the potential mechanism of EMT in pulmonary fibrosis. The obtained results showed that endoplasmic reticulum protein Nogo-b may promote MMP14-mediated proprotein maturation of TGF-β1, accelerating the release of free TGF-β1 in type II airway epithelial cells A549, subsquently, induce the epithelial-mesenchymal transition (EMT) of the cell...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29050257/linc-ror-induces-epithelial-to-mesenchymal-transition-in-ovarian-cancer-by-increasing-wnt-%C3%AE-catenin-signaling
#18
Yanhui Lou, Huanhuan Jiang, Zhumei Cui, Lingzhi Wang, Xiangyu Wang, Tian Tian
Long intergenic non-protein coding RNA, regulator of reprogramming (linc-ROR) is an intergenic long non-coding RNA (lncRNA) previously shown to contribute to tumorigenesis in several malignancies. However, little is known about whether linc-ROR has a role in ovarian cancer progression. In this study, we found that linc-ROR expression was increased in high-grade ovarian serous cancer tissues compared with normal ovarian tissues or normal fallopian tube tissues. Furthermore, the level of linc-ROR expression was associated with ovarian cancer International Federation of Gynecology and Obstetrics stage and lymph node metastasis...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29028618/one-single-standard-substance-for-the-simultaneous-determination-of-17-triterpenes-in-ganoderma-lingzhi-and-its-related-species-using-high-performance-liquid-chromatography
#19
Wei Liu, Jingsong Zhang, Wei Han, Yanfang Liu, Jie Feng, Chuanhong Tang, Na Feng, Qingjiu Tang
Due to the difficulty and high cost for the preparation of triterpenes, one single standard for the simultaneous determination of multi-components (SSDMC) with high performance liquid chromatography (HPLC) is an advanced solution for multi-component analysis. Experiments were carried out to investigate the feasibility of SSDMC for the analysis of Ganoderma triterpenes, with external standard method (ESM) compared, and the samples of Ganoderma were classified by the content of Ganoderma triterpenes. The analysis was performed by using a Fortis Speed Core-C18 column (150mm×4...
October 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29024267/novel-t3ss-effector-esek-in-edwardsiella-piscicida-is-chaperoned-by-esch-and-escs-to-express-virulence
#20
Huifang Cao, Cuiting Yang, Shu Quan, Tianjian Hu, Lingzhi Zhang, Yuanxing Zhang, Dahai Yang, Qin Liu
Bacterium usually utilizes type III secretion systems (T3SS) to deliver effectors directly into host cells with the aids of chaperones. Hence, it is very important to identify bacterial T3SS effectors and chaperones for better understanding of host-pathogen interactions. Edwardsiella piscicida is an invasive enteric bacterium, which infects a wide range of hosts from fish to human. Given E. piscicida encodes a functional T3SS to promote infection, very few T3SS effectors and chaperones have been identified in this bacterium so far...
October 11, 2017: Cellular Microbiology
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