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Lingzhi Zhao, Liu Zhao, Yanqing Miao, Chunye Liu, Chenxiao Zhang
The detection of pyrophosphatase (PPase) activity is of great significance in diagnosing diseases and understanding the function of PPase-related biological events. This study constructed a turn off-on-off fluorescent system for PPase activity assay based on PPase-regulated competitive coordination of Cu(2+) between a water-soluble fluorescent probe 6,7-dihydroxycoumarin (DHC) and pyrophosphate (PPi). The probe DHC can coordinate with Cu(2+) and consequently display on-off type fluorescence response. Furthermore, the in situ formed nonfluorescent Cu(2+)-DHC complex can act as an effective off-on type fluorescent probe for sensing PPi due to the higher coordination reactivity between Cu(2+) and PPi than that between Cu(2+) and DHC...
2016: Journal of Analytical Methods in Chemistry
Min Thura, Abdul Qader Omer Al-Aidaroos, Wei Peng Yong, Koji Kono, Abhishek Gupta, You Bin Lin, Kousaku Mimura, Jean Paul Thiery, Boon Cher Goh, Patrick Tan, Ross Soo, Cheng William Hong, Lingzhi Wang, Suling Joyce Lin, Elya Chen, Sun Young Rha, Hyun Cheol Chung, Jie Li, Sayantani Nandi, Hiu Fung Yuen, Shu-Dong Zhang, Yeoh Khay Guan, Jimmy So, Qi Zeng
Novel, tumor-specific drugs are urgently needed for a breakthrough in cancer therapy. Herein, we generated a first-in-class humanized antibody (PRL3-zumab) against PRL-3, an intracellular tumor-associated phosphatase upregulated in multiple human cancers, for unconventional cancer immunotherapies. We focused on gastric cancer (GC), wherein elevated PRL-3 mRNA levels significantly correlated with shortened overall survival of GC patients. PRL-3 protein was overexpressed in 85% of fresh-frozen clinical gastric tumor samples examined but not in patient-matched normal gastric tissues...
June 16, 2016: JCI Insight
Dan Xu, Yingshuo Wang, Zhimin Chen, Shuxian Li, Yungai Cheng, Li Zhang, Lingzhi Zhao
BACKGROUND: Asthma is a global problem. Prevalence varies among different countries and cities. We aimed to obtain the prevalence, describe the characteristics, and discover factors that may relate to asthma in Hangzhou. METHODS: This cross-sectional study was conducted in Hangzhou. The subjects were children aged 14 years and younger. A control group of non-asthma children that matched in age and sex with each asthmatic patient was also randomly selected and interviewed...
September 27, 2016: Respiratory Research
Zhongheng Zhang, Qian Li, Lingzhi Jiang, Bo Xie, Xiaowei Ji, Jiahong Lu, Ronglin Jiang, Shu Lei, Shihao Mao, Lijun Ying, Di Lu, Xiaoshui Si, Jianxin He, Mingxia Ji, Jianhua Zhu, Guodong Chen, Yadi Shao, Yinghe Xu, Ronghai Lin, Chao Zhang, Weiwen Zhang, Jian Luo, Tianzheng Lou, Xuwei He, Kun Chen, Renhua Sun
INTRODUCTION: Enteral feed is an important component of nutritional therapy in critically ill patients and underfeeding has been associated with adverse outcomes. The article developed an enteral feeding protocol and planed a before-and-after comparative trial to explore whether implementation of enteral feeding protocol was able to improve clinical outcomes. METHODS AND ANALYSIS: The study will be conducted in intensive care units (ICUs) of ten tertiary care academic centers...
August 2016: Annals of Translational Medicine
Wei Hong, Lingzhi Li, Ruinan Xue, Xiaoyang Xu, Huan Wang, Jingkuo Zhou, Huilin Zhao, Yahui Song, Yu Liu, Jianping Gao
Fabrication of low-cost and efficient electrocatalyst for oxygen reduction reaction (ORR) is highly desirable. Herein, Zinc ferrite/reduced graphene oxide (ZnFe2O4/rGO) is prepared by a quite simple and environmentally benign approach and applied as a high performance ORR electrocatalyst for the first time. The surface morphology and chemical composition of ZnFe2O4/rGO are characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, thermogravimetric analysis and Fourier transform infrared spectroscopy...
January 1, 2017: Journal of Colloid and Interface Science
Xiaoqing Tang, Weixi Cai, Baojun Xu
The objective of this study was to compare the mycochemical profiles, antioxidant activities, and antidiabetic effects of 2 species of genus Ganoderma, the red lingzhi (G. lucidum) and purple lingzhi (G. sinense) mushrooms. In Chinese medicinal practice, hot water and ethanol are used as solvents to extract samples. In this study, a total of 4 extracts (ethanol and hot water extracts from G. lucidum and G. sinense) were prepared for further assays. Hot water extracts presented much higher values for total phenolic content and ferric-reducing antioxidant power than the ethanol extracts...
2016: International Journal of Medicinal Mushrooms
Chun-Ru Cheng, Jie Ding, Yi Yang, Xin-Yong Liang, De-An Guo, Min Yang, Shu-Hong Guan
Ganoderma lucidum is a famous medicinal mushroom that has been widely used in clinical practice and as a dietary supplementa. The triterpenoid ganoderic acids are the main constituents of G. lucidum. To determine the pharmacokinetic characteristics of ganoderic acids, we developed and validated a sensitive and selective liquid chromatography-tandem mass spectrometry method to determine simultaneously the concentration of 4 representative ganoderic acids in rat plasma after oral administration of the extract from G...
2016: International Journal of Medicinal Mushrooms
Daichao Wu, Ming Guo, Michael A Philips, Lingzhi Qu, Longying Jiang, Jun Li, Xiaojuan Chen, Zhuchu Chen, Lin Chen, Yongheng Chen
Aberrant FGFR4 signaling has been documented abundantly in various human cancers. The majority of FGFR inhibitors display significantly reduced potency toward FGFR4 compared to FGFR1-3. However, LY2874455 has similar inhibition potency for FGFR1-4 with IC50 less than 6.4 nM. To date, there is no published crystal structure of LY2874455 in complex with any kinase. To better understand the pan-FGFR selectivity of LY2874455, we have determined the crystal structure of the FGFR4 kinase domain bound to LY2874455 at a resolution of 2...
2016: PloS One
Hongzhong Chen, Huijun Phoebe Tham, Chung Yen Ang, Qiuyu Qu, Lingzhi Zhao, Pengyao Xing, Linyi Bai, Si Yu Tan, Yanli Zhao
Targeted drug delivery systems having controlled drug release property with an inherent fluorescence reporter have drawn a lot of attention in nanomedicine. However, only very few prodrugs can be directly used to construct such delivery systems. Herein, we report that an amphiphilic chlorambucil-based prodrug consisting of a fluorescence reporter and a d-mannose targeting ligand could directly self-assemble into glutathione-responsive nanovesicles for selective cancer therapy and intracellular imaging. These nanovesicles could be dissociated to release the chlorambucil drug with obviously red-shifted fluorescence when internalized by d-mannose receptor-overexpressed MCF-7 cancer cells...
September 21, 2016: ACS Applied Materials & Interfaces
Chao Zhang, Shenghui Qin, Lingzhi Qin, Liwei Liu, Wenjia Sun, Xiyu Li, Naping Li, Renliang Wu, Xi Wang
Cigarette smoke exposure is a major cause of chronic obstructive pulmonary disease (COPD), but the underlying molecular inflammatory mechanisms remain poorly understood. Previous studies have found that smoke disrupts cell-cell adhesion by inducing epithelial barrier damage to the adherens junction proteins, primarily E-cadherin (E-cad) and p120-catenin (p120). Recently, the anti-inflammatory role of p120 has drawn increasing attention. In this study, we demonstrate that p120 has a role in the cigarette smoke extract-induced inflammatory response, presumably by regulating NF-κB signaling activation...
2016: Scientific Reports
Andrea L A Wong, Raghav Sundar, Ting-Ting Wang, Thian-C Ng, Bo Zhang, Sing-Huang Tan, Thomas I P Soh, Angela S L Pang, Chee-Seng Tan, Samuel G W Ow, Lingzhi Wang, Jannet Mogro, Jingshan Ho, Anand D Jeyasekharan, Yiqing Huang, Choon-Hua Thng, Ching-Wan Chan, Mikael Hartman, Philip Iau, Shaik A Buhari, Boon-Cher Goh, Soo-Chin Lee
BACKGROUND: Prolonged anti-angiogenic therapy destroys tumor vasculature, whereas vascular-normalizing doses may enhance intra-tumoral drug delivery. We hypothesize that low-dose, short-course sunitinib normalizes vasculature, enhancing chemotherapy efficacy. PATIENTS AND METHODS: In phase Ib, treatment-naïve breast cancer patients received four cycles of pre-operative doxorubicin/cyclophosphamide, with sunitinib before each cycle. The optimal dose of sunitinib leading to tumor vessel normalization on immunohistochemistry was identified...
August 25, 2016: Oncotarget
Seiichi Sakamoto, Nao Kikkawa, Toshitaka Kohno, Kuniyoshi Shimizu, Hiroyuki Tanaka, Satoshi Morimoto
Ganoderic acid A (GAA) is one of the major Ganoderma triterpenes produced by medicinal mushroom belonging to the genus Ganoderma (Ganodermataceae). Due to its interesting pharmacological activities, Ganoderma species have been traditionally used in China for the treatment of various diseases. Herein, we developed a colloidal gold-based immunochromatographic strip assay (ICA) for the rapid detection of GAA using highly specific monoclonal antibody against GAA (MAb 12A) conjugated with gold nanoparticles. Using the developed ICA, the detection of GAA can be completed within 15min after dipping the test strip into an analyte solution with the limit of detection (LOD) for GAA of ~500ng/mL...
October 2016: Fitoterapia
Huabing Liu, Chaoyong Xu, Yanli Bai, Lin Liu, Dongmei Liao, Jiangong Liang, Lingzhi Liu, Heyou Han
Carbon dots (CDs) have been widely used for the preparation of multifunctional probes by conjugation with organic fluorescent dyes. However, the effect of organic fluorescent dyes on CDs still remains poorly understood. Herein, the effect of fluorescein isothiocyanate (FITC) on CDs was explored by spectroscopic techniques at pH5.1, 7.0 and 9.0. The fluorescent intensity of CDs was found to be quenched gradually after mixing directly with different concentrations of FITC, but the fluorescent lifetime of CDs remained unchanged...
January 15, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Daichao Wu, Lingzhi Qu, Yang Fu, Jun Li, Longying Jiang, Xiaojuan Chen, Ming Guo, Zhuchu Chen, Lin Chen, Yongheng Chen
PTEN-induced putative kinase 1 (PINK1) is a Ser/Thr kinase that specifically localizes on the mitochondrial membrane. It cooperates with Parkin to regulate mitochondrial quality control. Mutations in PINK1 protein which account for 8-15% of Parkinson's disease (PD), are the second most common cause of early-onset Autosomal Recessive Parkinson's disease (AR-PD). The lack of methods for PINK1 heterologous expression and purification has slowed progress in the AR-PD research field. To pave the way for direct structural study of this important protein, in this study, we developed an efficient expression system of recombinant PINK1 kinase domain (rPINK1) using Pichia pastoris (P...
December 2016: Protein Expression and Purification
Donghua Li, Yurong Yan, Lingzhi Yu, Yong Duan
Neuropathic pain (NPP) is the main culprit among chronic pains affecting the normal life of patients. Procaine is a frequently-used local anesthesia with multiple efficacies in various diseases. However, its role in modulating NPP has not been reported yet. This study aims at uncovering the role of procaine in NPP. Rats were pretreated with procaine by intrathecal injection. Then NPP rat model was induced by sciatic nerve chronic compression injury (CCI) and behavior tests were performed to analyze the pain behaviors upon mechanical, thermal and cold stimulations...
September 1, 2016: Biomolecules & Therapeutics
Simon Siu-Man Sum, Jane Ziegler
No abstract text is available yet for this article.
October 2016: Nutrition in Clinical Practice
Yanxia Zhu, Kedong Song, Siyu Jiang, Jinglian Chen, Lingzhi Tang, Siyuan Li, Jiangli Fan, Yiwei Wang, Jiaquan Zhao, Tianqing Liu
Cartilage tissue engineering is believed to provide effective cartilage repair post-injuries or diseases. Biomedical materials play a key role in achieving successful culture and fabrication of cartilage. The physical properties of a chitosan/gelatin hybrid hydrogel scaffold make it an ideal cartilage biomimetic material. In this study, a chitosan/gelatin hybrid hydrogel was chosen to fabricate a tissue-engineered cartilage in vitro by inoculating human adipose-derived stem cells (ADSCs) at both dynamic and traditional static culture conditions...
August 15, 2016: Applied Biochemistry and Biotechnology
Hongliang Liu, Changchun Hou, Qiang Zeng, Liang Zhao, Yushan Cui, Linyu Yu, Lingzhi Wang, Yang Zhao, Junyan Nie, Bin Zhang, Aiguo Wang
Excess fluoride and iodide coexist in drinking water in many regions, but few studies have investigated the single or interactive effects on thyroid in vivo. In our study, Wistar rats were exposed to excess fluoride and/or iodide through drinking water for 2 or 8 months. The structure and function of the thyroid, cells apoptosis and the expression of inositol-requiring enzyme 1 (IRE1) pathway-related factors were analyzed. Results demonstrated that excess fluoride and/or iodide could change thyroid follicular morphology and alter thyroid hormone levels in rats...
September 2016: Environmental Toxicology and Pharmacology
Lingzhi Huang, Lezhi Li, Xiaofang Chen, Huiling Zhang, Zeya Shi
BACKGROUND: Acute myocardial infarction (AMI) is commonly known as the heart attack. The molecular events involved in the development of AMI remain unclear. This study was to investigate the expression of miR-103a in patients with high blood pressure (HBP) and AMI patients with and without HBP, as well as its effect on endothelial cell functions. METHODS: MiR-103a expression in plasma and peripheral blood mononuclear cells was measured by real-time polymerase chain reaction (PCR)...
August 12, 2016: Cardiology Journal
Fei Gao, Linyi Bai, Xiaohua Feng, Huijun Phoebe Tham, Ruochong Zhang, Yuanyuan Zhang, Siyu Liu, Lingzhi Zhao, Yuanjin Zheng, Yanli Zhao
Two near-infrared dyes featuring good dispersion and light-harvesting property present a remarkable nonlinear photoacoustic response in vitro and in vivo comparing with conventional gold nanorods. This study benefits the fabrication of drug delivery platforms with accurate targeting and control effect under photoacoustic image guidance.
October 2016: Small
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