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Hematopoieticstem cell

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https://www.readbyqxmd.com/read/27956370/flow-cytometric-aldehyde-dehydrogenase-assay-enables-a-fast-and-accurate-human-umbilical-cord-blood-hematopoietic-stem-cell-assessment
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Emine Begüm Gençer, Pınar Yurdakul, Klara Dalva, Meral Beksaç
OBJECTIVE:  Colony-forming units of granulocytes/macrophages (CFU-GM) analysis is the most widely used method to determine the hematopoietic stem cell (HSC) content of human umbilical cord blood (CB) for prediction of engraftment potential. The measurement of aldehyde dehydrogenase (ALDH) activity is a more recent method for HSC qualification. Our aim was to correlate phenotypic and functional assays to find the most predictive method. MATERIALS AND METHODS:  In this study, flow cytometric quantitation of CD34+ cells and ALDH positivity along with CFU-GM capacity were assessed in fresh and post-thaw CB units...
December 1, 2017: Turkish Journal of Haematology: Official Journal of Turkish Society of Haematology
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