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Hematopoieticstem cell

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https://www.readbyqxmd.com/read/27956370/flow-cytometric-aldehyde-dehydrogenase-aldh-assay-enables-a-fast-and-accurate-human-umblical-cord-blood-hematopoietic-stem-cell-assessment
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Emine Begüm Gencer, Pınar Yurdakul, Klara Dalva, Meral Beksac
OBJECTIVE: Colony forming unit-granulocyte macrophage (CFU-GM) analysis is the most widely used method to determine hematopoieticstem cell (HSC) content of human umbilical cord blood (CB) for prediction of engraftment potential. Measurement of aldehyde dehydrogenase (ALDH) activity is a more recent method for HSC qualification. Our aim was to correlate phenotypic with functional assays to find the most predictive method. MATERIALS AND METHODS: In this study, flow cytometric quantitation of CD34+ cells and ALDH positivity along with CFU-GM capacity were assessed in fresh and post thaw CB units...
December 12, 2016: Turkish Journal of Haematology: Official Journal of Turkish Society of Haematology
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