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Michael Scherer, Indranil Singh, Martina Braun, Chelsea Szu-Tu, Michael Kardorff, Julia Rühle, Robert Frömel, Sergi Beneyto-Calabuig, Simon Raffel, Alejo Rodriguez-Fraticelli, Lars Velten
Current approaches to lineage tracing of stem cell clones require genetic engineering or rely on sparse somatic DNA variants, which are difficult to capture at single-cell resolution. Here, we show that targeted single-cell measurements of DNA methylation at single-CpG resolution deliver joint information about cellular differentiation state and clonal identities. We develop EPI-clone, a droplet-based method for transgene-free lineage tracing, and apply it to study hematopoiesis, capturing hundreds of clonal trajectories across almost 100,000 single-cells...
April 1, 2024: bioRxiv
#2
JOURNAL ARTICLE
Neftalí Ochoa-Alejo, M Humberto Reyes-Valdés, Octavio Martínez
Chili pepper fruits of the genus Capsicum represent excellent experimental models to study the growth, development, and ripening processes in a non-climacteric species at the physiological, biochemical, and molecular levels. Fruit growth, development, and ripening involve a complex, harmonious, and finely controlled regulation of gene expression. The purpose of this study was to estimate the changes in transcriptome diversity and specialization, as well as gene specificities during fruit development in this crop, and to illustrate the advantages of estimating these parameters...
March 29, 2024: Plants (Basel, Switzerland)
#3
REVIEW
Diego M Presman, Belén Benítez, Agustina L Lafuente, Alejo Vázquez Lareu
Eukaryotic genomes store information on many levels, including their linear DNA sequence, the posttranslational modifications of its constituents (epigenetic modifications), and its three-dimensional folding. Understanding how this information is stored and read requires multidisciplinary collaborations from many branches of science beyond biology, including physics, chemistry, and computer science. Concurrent recent developments in all these areas have enabled researchers to image the genome with unprecedented spatial and temporal resolution...
April 12, 2024: Histochemistry and Cell Biology
#4
JOURNAL ARTICLE
David Barranco-Gil, Lidia B Alejo, Carlos Revuelta, Miguel Górriz, Itziar Pagola, Laureano M Ozcoidi, Alejandro Lucia, Pedro L Valenzuela
BACKGROUND: Growing evidence supports the ergogenic effects of creatine supplementation on muscle power/strength, but its effects on endurance performance remain unclear. We assessed the effects of high-dose short-term creatine supplementation in professional cyclists during a training camp. METHODS: The study followed a double-blind, randomized parallel design. Twenty-three professional U23 cyclists (19 ± 1 years, maximum oxygen uptake: 73...
December 2024: Journal of the International Society of Sports Nutrition
#5
JOURNAL ARTICLE
Masuma Chowdhury, Alejo Martínez-Sansigre, Maruška Mole, Eduardo Alonso-Peleato, Nadiia Basos, Jose Manuel Blanco, Maria Ramirez-Nicolas, Isabel Caballero, Ignacio de la Calle
Seagrasses are undergoing widespread loss due to anthropogenic pressure and climate change. Since 1960, the Mediterranean seascape lost 13-50% of the areal extent of its dominant and endemic seagrass-Posidonia oceanica, which regulates its ecosystem. Many conservation and restoration projects failed due to poor site selection and lack of long-term monitoring. Here, we present a fast and efficient operational approach based on a deep-learning artificial intelligence model using Sentinel-2 data to map the spatial extent of the meadows, enabling short and long-term monitoring, and identifying the impacts of natural and human-induced stressors and changes at different timescales...
April 10, 2024: Scientific Reports
#6
JOURNAL ARTICLE
Brenda Carolina Guerra-Alejos, Youwei Yan, Megan Kurz, Nishan Mudalige, Jeong Eun Min, Fahmida Homayra, Bohdan Nosyk
BACKGROUND AND AIM: In British Columbia, Canada, clinical guidelines for the treatment of opioid use disorders (OUD) were updated in 2017, during a period in which the potency and composition of the illicit drug supply changed rapidly. We aimed to describe changes in opioid agonist treatment (OAT) prescribing practices at the population level in a setting in which fentanyl and its analogs have become the primary illicit opioid of use. DESIGN, SETTING AND PARTICIPANTS: This was a population-based retrospective cohort study using three linked health administrative databases in British Columbia (BC), Canada...
April 7, 2024: Addiction
#7
JOURNAL ARTICLE
Alexis Carteron, Isabel Cantera, Alessia Guerrieri, Silvio Marta, Aurélie Bonin, Roberto Ambrosini, Fabien Anthelme, Roberto Sergio Azzoni, Peter Almond, Pablo Alviz Gazitúa, Sophie Cauvy-Fraunié, Jorge Luis Ceballos Lievano, Pritam Chand, Milap Chand Sharma, John J Clague, Justiniano Alejo Cochachín Rapre, Chiara Compostella, Rolando Cruz Encarnación, Olivier Dangles, Andre Eger, Sergey Erokhin, Andrea Franzetti, Ludovic Gielly, Fabrizio Gili, Mauro Gobbi, Sigmund Hågvar, Norine Khedim, Rosa Isela Meneses, Gwendolyn Peyre, Francesca Pittino, Antoine Rabatel, Nurai Urseitova, Yan Yang, Vitalii Zaginaev, Andrea Zerboni, Anaïs Zimmer, Pierre Taberlet, Guglielmina Adele Diolaiuti, Jerome Poulenard, Wilfried Thuiller, Marco Caccianiga, Gentile Francesco Ficetola
The development of terrestrial ecosystems depends greatly on plant mutualists such as mycorrhizal fungi. The global retreat of glaciers exposes nutrient-poor substrates in extreme environments and provides a unique opportunity to study early successions of mycorrhizal fungi by assessing their dynamics and drivers. We combined environmental DNA metabarcoding and measurements of local conditions to assess the succession of mycorrhizal communities during soil development in 46 glacier forelands around the globe, testing whether dynamics and drivers differ between mycorrhizal types...
April 6, 2024: New Phytologist
#8
REVIEW
Javier Gutiérrez-Guisado, Alejo Erice Calvo-Sotelo, Luis Hernández-Blasco, Ángeles Fidalgo, Covadonga Gómez-Cuervo, Antonio López-Ruiz, Jesús Aibar, Peter Verhamme, José Meireles, Manuel Monreal
BACKGROUND: Venous thromboembolism (VTE) is a critical complication after non-major trauma or surgery. While the risk and severity of VTE following major orthopedic surgery is well-documented, there is significant knowledge gap regarding, non-major trauma such as ankle sprains. METHODS: We analyzed data from the RIETE registry to assess the clinical characteristics, VTE prophylaxis usage, and outcomes in patients with VTE following ankle sprain versus those post elective knee arthroplasty...
March 21, 2024: Thrombosis Research
#9
JOURNAL ARTICLE
J Gil-Lianes, G Tena, M Combalia, B Alejo, G Oses, A Combalia, P Iglesias, A Huguet, S Garrido, J Sola, J Malvehy, M Mollà, C Carrera
BACKGROUND: acute radiation dermatitis (ARD) is the most widely reported radiotherapy-induced adverse event. Currently, there is no objective or reliable method to measure ARD. OBJECTIVE: our main objective was to identify and quantify the effects of radiotherapy with a computational model using optical coherence tomography (OCT) skin scanning. Secondary objectives included determining the ARD impact of different radiotherapeutic schemes and adjuvant topical therapies...
March 28, 2024: Actas Dermo-sifiliográficas
#10
JOURNAL ARTICLE
Alexander Marschall, Juan Duarte Torres, Belén Biscotti Rodíl, Inés Gómez Sánchez, Elena Basabe Velasco, Carmen Ramos Alejos-Pita, Edurne López Soberón, Alfonso Suárez Cuervo, Salvador Álvarez Antón, Jose María de la Torre Hernández, David Martí Sánchez
Elderly patients have been remarkably under-represented in bleeding risk cohorts. Thus, the PRECISE-DAPT and ARC-HBR scores are not validated in older adults. Therefore, we sought to evaluate the PRECISE-DAPT and ARC-HBR scores in an exclusively elderly population and furthermore assess the prognostic value of a truly simplified clinical evaluation (SCE), consisting of only 3 binary clinical variables (hemoglobin <11g/dL, prior bleeding and anticipated use of anticoagulants). This is a retrospective analysis of the prospective single-center Elderly-HCD registry...
March 27, 2024: American Journal of Cardiology
#11
JOURNAL ARTICLE
Alejo Ramiro Barbuzza, Fabricio Ballarini, Celina Goyeneche, Victoria Reppucci, Pedro Benedetti, Franco Moscato, Jorge H Medina, Cynthia Katche, Diego Moncada, Haydeé Viola
Background and objectives A controversy regarding the duration of generalized anxiety disorders (GAD) and depressive symptoms during the COVID-19 pandemic arose, stating that these symptoms last a short time, perhaps a few months, or that they are more persistent over time. After more than three years of the pandemic, this is still a question that requires an answer. The main goal of this work was to record the levels of self-perceived GAD and depression in the Argentine population at several time points during the pandemic to characterize whether they were transient or persisted over the successive waves of contagion...
February 2024: Curēus
#12
JOURNAL ARTICLE
Víctor Rodríguez-Rielves, David Barranco-Gil, Ángel Buendía-Romero, Alejandro Hernández-Belmonte, Enrique Higueras-Liébana, Jon Iriberri, Iván R Sánchez-Redondo, José Ramón Lillo-Beviá, Alejandro Martínez-Cava, Raúl de Pablos, Pedro L Valenzuela, Jesús G Pallarés, Lidia B Alejo
We aimed to determine the feasibility, test-retest reliability and long-term stability of a novel method for assessing the force (torque)-velocity (cadence) profile and maximal dynamic force (MDF) during leg-pedaling using a friction-loaded isoinertial cycle ergometer and a high-precision power-meter device. Fifty-two trained male cyclists completed a progressive loading test up to the one-repetition maximum (1RM) on a cycle ergometer. The MDF was defined as the force attained at the cycle performed with the 1RM-load...
March 21, 2024: Sensors
#13
JOURNAL ARTICLE
Gabriela Pavarini, David M Lyreskog, Danielle Newby, Jessica Lorimer, Vanessa Bennett, Edward Jacobs, Laura Winchester, Alejo Nevado-Holgado, Ilina Singh
BACKGROUND: Use of personal sensing to predict mental health risk has sparked interest in adolescent psychiatry, offering a potential tool for targeted early intervention. OBJECTIVES: We investigated the preferences and values of UK adolescents with regard to use of digital sensing information, including social media and internet searching behaviour. We also investigated the impact of risk information on adolescents' self-understanding. METHODS: Following a Design Bioethics approach, we created and disseminated a purpose-built digital game (www...
March 20, 2024: BMJ Ment Health
#14
JOURNAL ARTICLE
Ana Belén Plata-Gómez, Lucía de Prado-Rivas, Alba Sanz, Nerea Deleyto-Seldas, Fernando García, Celia de la Calle Arregui, Camila Silva, Eduardo Caleiras, Osvaldo Graña-Castro, Elena Piñeiro-Yáñez, Joseph Krebs, Luis Leiva-Vega, Javier Muñoz, Ajay Jain, Guadalupe Sabio, Alejo Efeyan
The metabolic functions of the liver are spatially organized in a phenomenon called zonation, linked to the differential exposure of portal and central hepatocytes to nutrient-rich blood. The mTORC1 signaling pathway controls cellular metabolism in response to nutrients and insulin fluctuations. Here we show that simultaneous genetic activation of nutrient and hormone signaling to mTORC1 in hepatocytes results in impaired establishment of postnatal metabolic and zonal identity of hepatocytes. Mutant hepatocytes fail to upregulate postnatally the expression of Frizzled receptors 1 and 8, and show reduced Wnt/β-catenin activation...
March 18, 2024: Nature Communications
#15
REVIEW
Diana Sagastume, Antonio Barrenechea-Pulache, Andrea Ruiz-Alejos, Katja Polman, Lenka Beňová, Manuel Ramírez-Zea, José L Peñalvo
BACKGROUND: Estimating the prevalence of double burden of malnutrition (DBM) is challenging in the Latin American and Caribbean (LAC) region where various DBM typologies (e.g., obesity and stunting) are heterogeneous and estimates are scattered across literature. OBJECTIVES: We aimed to assess the prevalence of DBM typologies in the LAC region. METHODS: We searched PubMed, Embase, Scopus, and Web of Science to identify studies on the prevalence of DBM published between 1 January, 2000, and 23 January, 2023...
March 15, 2024: Advances in Nutrition
#16
JOURNAL ARTICLE
Matthew S Tenan, Bob Alejo
There are two largely competing models for an athletics organization at both the collegiate and professional levels: the High Performance Model and the Medical Model. The High Performance Model arises from international Football perspective that places a "Performance Director" at the center of teams supporting the athletes. The Medical Model, supported by both the National Athletic Trainers Association and the NCAA, separates off medical staff (athletic trainers, physical therapists, and physicians, predominantly) and emphasizes the autonomy of medical decisions...
March 13, 2024: Journal of Athletic Training
#17
F Aaron Cruz-Navarrete, Wezley C Griffin, Yuk-Cheung Chan, Maxwell I Martin, Jose L Alejo, S Kundhavai Natchiar, Isaac J Knudson, Roger B Altman, Alanna Schepartz, Scott J Miller, Scott C Blanchard
Templated synthesis of proteins containing non-natural amino acids (nnAAs) promises to vastly expand the chemical space available to biological therapeutics and materials. Existing technologies limit the identity and number of nnAAs than can be incorporated into a given protein. Addressing these bottlenecks requires deeper understanding of the mechanism of messenger RNA (mRNA) templated protein synthesis and how this mechanism is perturbed by nnAAs. Here we examine the impact of both monomer backbone and side chain on formation and ribosome-utilization of the central protein synthesis substate: the ternary complex of native, aminoacylated transfer RNA (aa-tRNA), thermally unstable elongation factor (EF-Tu), and GTP...
February 27, 2024: bioRxiv
#18
JOURNAL ARTICLE
Carmen Alicia Ruiz Valdez, Olga María Alejo Martínez, Brenda Leticia Rocha Reyes, Porfirio Felipe Hernández Bautista, David Alejandro Cabrera Gaytán, Alfonso Vallejos Parás, Lumumba Arriaga Nieto, Leticia Jaimes Betancourt, Alejandro Moctezuma Paz, Mónica Grisel Rivera Mahey, Gabriel Valle Alvarado, Brenda Ivett Velez García
INTRODUCTION: Dengue is a disease with a wide clinical spectrum. The early identification of dengue cases is crucial but challenging for health professionals; therefore, it is necessary to have effective diagnostic instruments to initiate timely care. OBJECTIVE: To evaluate the effectiveness of an algorithm based on an artificial neural network (ANN) to diagnose dengue in an endemic area. METHODS: A single-center case-control study was conducted in a secondary-care hospital in Ciudad Obregón, Sonora...
2024: Digital Health
#19
JOURNAL ARTICLE
Federico Giannitti, Mizael Machado, Caroline da Silva Silveira, Ximena Cibils-Stewart, Nicolás Baráibar, Cintia R R Queiroz-Machado, Robert H Poppenga, Alejo Menchaca, Francisco A Uzal, Juan A García, Carolina Matto, Fernando Dutra, Gretel Ruprechter, Darío Caffarena, Anderson Saravia
In the summer of 2023, ingestion of Astylus atromaculatus (pollen beetle) was linked to spontaneous fatal disease in grazing cattle and sheep in Argentina and Uruguay. While the disease was experimentally reproduced in sheep and guinea pigs in the 1970's, no experimental reproductions have been attempted in cattle, and controversy exists as to whether this insect is indeed noxious to cattle and at which dose. Here, we demonstrate that A. atromaculatus causes acute fatal disease in Hereford calves at single oral dosages of 2...
March 4, 2024: Veterinary Pathology
#20
JOURNAL ARTICLE
Natalia Jiménez, Marta Garcia de Herreros, Òscar Reig, Mercedes Marín-Aguilera, Caterina Aversa, Laura Ferrer-Mileo, Samuel García-Esteve, Leonardo Rodríguez-Carunchio, Isabel Trias, Albert Font, Alejo Rodriguez-Vida, Miguel Ángel Climent, Sara Cros, Isabel Chirivella, Montserrat Domènech, Mariona Figols, Joan Carles, Cristina Suárez, Daniel Herrero Rivera, Enrique González-Billalabeitia, Claudia Cívico, Núria Sala-González, Vicenç Ruiz de Porras, Maria J Ribal, Aleix Prat, Begoña Mellado
BACKGROUND: Androgen deprivation therapy (ADT) with docetaxel (D) and/or antiandrogen receptor therapies (ARTs) are the standard therapies in metastatic hormone-sensitive prostate cancer (mHSPC). Alterations in the tumor suppressor genes (TSGs) RB1, PTEN, and TP53 are associated with an aggressive evolution and treatment resistance in castration-resistant prostate cancer (CRPC). OBJECTIVE: To study the clinical implications of TSG mRNA expression in mHSPC patients...
March 1, 2024: European Urology Oncology
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