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https://www.readbyqxmd.com/read/28208769/localisation-microscopy-of-breast-epithelial-erbb-2-receptors-and-gap-junctions-trafficking-after-%C3%AE-irradiation-neuregulin-1%C3%AE-and-trastuzumab-application
#1
Götz Pilarczyk, Ines Nesnidal, Manuel Gunkel, Margund Bach, Felix Bestvater, Michael Hausmann
In cancer, vulnerable breast epithelium malignance tendency correlates with number and activation of ErbB receptor tyrosine kinases. In the presented work, we observe ErbB receptors activated by irradiation-induced DNA injury or neuregulin- 1 β application, or alternatively, attenuated by a therapeutic antibody using high resolution fluorescence localization microscopy. The gap junction turnover coinciding with ErbB receptor activation and co-transport is simultaneously recorded. DNA injury caused by 4 Gray of 6 MeV photon γ -irradiation or alternatively neuregulin- 1 β application mobilized ErbB receptors in a nucleograde fashion-a process attenuated by trastuzumab antibody application...
February 9, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28208094/a-simple-strategy-to-refine-cu2o-photocatalytic-capacity-for-refractory-pollutants-removal-roles-of-oxygen-reduction-and-fe-ii-chemistry
#2
Ai-Yong Zhang, Yuan-Yi He, Tan Lin, Nai-Hui Huang, Qiao Xu, Jing-Wei Feng
Visible-light-driven photocatalysis is a promising technology for advanced water treatment, but it usually exhibits a low efficiency. Cu2O is a low-cost semiconductor with narrow band gap, high absorption coefficient and suitable conduction band, but suffers from low charge mobility, poor quantum yield and weak catalytic performance. Herein, the Cu2O catalytic capacity for refractory pollutants degradation is drastically improved by a simple and effective strategy. By virtue of the synergistic effects between photocatalysis and Fenton, a novel and efficient photocatalysis-driven Fenton system, PFC, is originally proposed and experimentally validated using Cu2O/Nano-C hybrids...
January 29, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28207192/temporal-spatial-establishment-of-initial-niche-for-the-primary-spermatogonial-stem-cell-formation-is-determined-by-an-arid4b-regulatory-network
#3
Ray-Chang Wu, Yang Zeng, Yu-Fang Chen, Rainer B Lanz, Mei-Yi Wu
During neonatal testis development, centrally located gonocytes migrate to basement membrane of the seminiferous cords, where physical contact with a niche established by Sertoli cells is essential for transition of gonocytes into spermatogonial stem cells (SSCs). To provide structural support and signaling stimuli for the gonocyte-to-SSC transition that occurs at a specific location during a finite phase, temporal-spatial establishment of the niche is critical. To date, the factors that guide Sertoli cells to establish the initial stem cell niche remain largely unknown...
February 16, 2017: Stem Cells
https://www.readbyqxmd.com/read/28205646/connexin30-3-is-expressed-in-mouse-embryonic-stem-cells-and-is-responsive-to-leukemia-inhibitory-factor
#4
Mikako Saito, Yuma Asai, Keiichi Imai, Shoya Hiratoko, Kento Tanaka
The expression of 19 connexin (Cx) isoforms was observed in the mouse embryonic stem (ES) cell line, EB3. Their expression patterns could be classified into either pluripotent state-specific, differentiating stage-specific, or non-specific Cxs. We focused on Cx30.3 as typical of the first category. Cx30.3 was pluripotent state-specific and upregulated by leukemia inhibitory factor (LIF), a specific cytokine that maintains the pluripotent state of ES cell, via a Jak signaling pathway. Cx30.3 protein was localized to both the cell membrane and cytosol...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28205211/unraveling-a-novel-mechanism-for-the-up-regulation-of-connexin43-gap-junctions-between-cells-derived-from-the-blood-brain-barrier
#5
Juan C Sáez
Endothelial cells from different vascular territories in mammals express at least three connexins (Cxs: Cx37, Cx40 and Cx43), which are protein subunits of gap junction channels. This article is protected by copyright. All rights reserved.
February 15, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28202692/localization-of-phosphorylated-connexin-43-by-serial-section-immunogold-electron-microscopy
#6
Rachael P Norris, Valentina Baena, Mark Terasaki
Gap junction turnover occurs by the internalization of both plasma membranes of a gap junction plaque to form a double membrane-enclosed vesicle, or connexosome. Phosphorylation has a key role in regulation, but further progress requires clearly distinguishing gap junctions and connexosomes and precisely localizing proteins to them. We examined by electron microscopy serial sections of mouse preovulatory ovarian follicles collected with an automated tape collecting ultramicrotome (ATUM). We found connexosomes may form from adjacent cell bodies, from thin cell processes, or from the same cell...
February 15, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28202194/study-of-factors-affecting-the-progression-and-termination-of-drug-induced-torsade-de-pointes-in-two-dimensional-cardiac-tissue
#7
Ponnuraj Kirthi Priya, M Ramasubba Reddy
INTRODUCTION: To study the conditions leading to the initiation and termination of drug induced Torsade de pointes (TdP) along with QT prolongation. METHODS: A 2D anisotropic transmural section of the ventricular myocardium is modeled using the TP06 equations and the cells are interconnected with gap junction conductances (GJC). The tissue is remodeled by reducing the repolarization reserve (by increasing calcium current (ICaL)) of all cells thus making them vulnerable to development of early after depolarizations (EADs)...
February 2, 2017: Journal of Electrocardiology
https://www.readbyqxmd.com/read/28199220/critical-analysis-of-soft-point-contact-andreev-reflection-spectra-between-superconducting-films-and-pressed-in
#8
Pradnya Parab, Prashant Chauhan, H Muthurajan, Sangita Bose
We present a critical analysis of an alternative technique of point contact Andreev reflection (PCAR) spectroscopy used to extract energy resolved information of superconductors which is based on making 'soft-contacts' between superconductors and indium. This technique is not sensitive to mechanical vibrations and hence can be used in a cryogen free platform increasing its accessibility to users having no access to cryogenic liquids. Through our experiments on large number of superconducting films we show that the PCAR spectra below the T c of In show sub-harmonic gap structures consistent with the theory of multiple Andreev reflection (MAR) and a zero bias conductance (ZBC) anomaly associated with the Josephson supercurrent...
February 15, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/28193563/calcium-oxidative-stress-and-connexin-channels-a-harmonious-orchestra-directing-the-response-to-radiotherapy-treatment
#9
REVIEW
Elke Decrock, Delphine Hoorelbeke, Raghda Ramadan, Tinneke Delvaeye, Marijke De Bock, Nan Wang, Dmitri V Krysko, Sarah Baatout, Geert Bultynck, An Aerts, Mathieu Vinken, Luc Leybaert
Although radiotherapy is commonly used to treat cancer, its beneficial outcome is frequently hampered by the radiation resistance of tumor cells and adverse reactions in normal tissues. Mechanisms of cell-to-cell communication and how intercellular signals are translated into cellular responses, have become topics of intense investigation, particularly within the field of radiobiology. A substantial amount of evidence is available demonstrating that both gap junctional and paracrine communication pathways can propagate radiation-induced biological effects at the intercellular level, commonly referred to as radiation-induced bystander effects (RIBE)...
February 10, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28193317/identification-of-differentially-expressed-placental-transcripts-during-multiple-gestations-in-the-eurasian-beaver-castor-fiber-l
#10
A Lipka, L Paukszto, M Majewska, J P Jastrzebski, K Myszczynski, G Panasiewicz, B Szafranska
The Eurasian beaver is one of the largest rodents that, despite its high impact on the environment, is a non-model species that lacks a reference genome. Characterising genes critical for pregnancy outcome can serve as a basis for identifying mechanisms underlying effective reproduction, which is required for the success of endangered species conservation programs. In the present study, high-throughput RNA sequencing (RNA-seq) was used to analyse global changes in the Castor fiber subplacenta transcriptome during multiple pregnancy...
February 14, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28192031/neural-crest-stem-cells-can-differentiate-to-a-cardiomyogenic-lineage-with-an-ability-to-contract-in-response-to-pulsed-infrared-stimulation
#11
Jordan M Greenberg, Vicente Lumbreras, Daniel Pelaez, Suhrud M Rajguru, Herman S Cheung
INTRODUCTION: Cellular cardiomyoplasty has rapidly risen to prominence in the clinic following a myocardial infarction; however, low engraftment of transplanted cells limits the therapeutic benefit to these procedures. Recently, lineage-specific stem cells differentiated into cardiomyocytes have gained much attention to assist in the repair of an injured heart tissue; however, questions regarding the ideal cell source remain. In the present study, we have identified a source that is easy to extract stem cells from and show that the cells present have a high plasticity toward the cardiomyogenic lineage...
October 2016: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28190758/intercellular-signalling-cross-talk-to-kill-to-heal-and-to-rejuvenate
#12
REVIEW
Egor Y Plotnikov, Denis N Silachev, Vasily A Popkov, Ljubava D Zorova, Irina B Pevzner, Savva D Zorov, Stanislovas S Jankauskas, Valentina A Babenko, Gennady T Sukhikh, Dmitry B Zorov
Intercellular cross-talk is a fundamental process for spreading cellular signals between neighbouring and distant cells to properly regulate their metabolism, to coordinate homeostasis, adaptation and survival as a functional tissue and organ. In this review, we take a close molecular view of the underpinning molecular mechanisms of such complex intercellular communications. There are several studied forms of cell-to-cell communications considered crucial for the maintenance of multicellular organisms. The most explored is paracrine signalling which is realised through the release of diffusible signalling factors (e...
January 23, 2017: Heart, Lung & Circulation
https://www.readbyqxmd.com/read/28188112/thrombin-induces-inositol-trisphosphate-mediated-spatially-extensive-responses-in-lung-microvessels
#13
Rachel Escue, Kathirvel Kandasamy, Kaushik Parthasarathi
Activation of plasma membrane receptors initiates compartmentalized second messenger signaling. Whether this compartmentalization facilitates the preferential intercellular diffusion of specific second messengers is unclear. Toward this, the receptor-mediated agonist, thrombin, was instilled into microvessels in a restricted region of isolated blood-perfused mouse lungs. Subsequently, the thrombin-induced increase in endothelial F-actin was determined using confocal fluorescence microscopy. Increased F-actin was evident in microvessels directly treated with thrombin and in those located in adjoining thrombin-free regions...
February 7, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28187910/analysis-of-gap-junctional-intercellular-communications-using-a-dielectrophoresis-based-microchip
#14
Marta Tellez-Gabriel, Céline Charrier, Bénédicte Brounais-Le Royer, Mathilde Mullard, Hannah K Brown, Franck Verrecchia, Dominique Heymann
Gap junctions are transmembrane structures that directly connect the cytoplasm of adjacent cells, making intercellular communications possible. It has been shown that the behaviour of several tumours - such as bone tumours - is related to gap junction intercellular communications (GJIC). Several methodologies are available for studying GJIC, based on measuring different parameters that are useful for multiple applications, such as the study of carcinogenesis for example. These methods nevertheless have several limitations...
February 6, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28181063/connexin43-and-the-intercellular-signaling-network-regulating-skeletal-remodeling
#15
REVIEW
Megan C Moorer, Joseph P Stains
PURPOSE OF THE REVIEW: This review highlights recent developments into how intercellular communication through connexin43 facilitates bone modeling and remodeling. RECENT FINDINGS: Connexin43 is required for both skeletal development and maintenance, particularly in cortical bone, where it carries out multiple functions, including preventing osteoclastogenesis, restraining osteoprogenitor proliferation, promoting osteoblast differentiation, coordinating organized collagen matrix deposition, and maintaining osteocyte survival...
February 8, 2017: Current Osteoporosis Reports
https://www.readbyqxmd.com/read/28180214/detection-of-electron-tunneling-across-plasmonic-nanoparticle-film-junctions-using-nitrile-vibrations
#16
Hao Wang, Kun Yao, John A Parkhill, Zachary D Schultz
The significant electric field enhancements that occur in plasmonic nanogap junctions are instrumental in boosting the performance of spectroscopy, optoelectronics and catalysis. Electron tunneling, associated with quantum effects in small junctions, is reported to limit the electric field enhancement. However, observing and quantitatively determining how tunneling alters the electric fields within small gaps is challenging due to the nanoscale dimensions and heterogeneity present experimentally. Here, we report the use of a nitrile probe placed in the nanoparticle-film gap junctions to demonstrate that the change in the nitrile stretching band associated with the vibrational Stark effect can be directly correlated with the local electric field environment modulated by gap size variations...
February 9, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28179989/bystander-effects-elicited-by-single-cell-photo-oxidative-blue-light-stimulation-in-retinal-pigment-epithelium-cell-networks
#17
Masaaki Ishii, Bärbel Rohrer
'Bystander effect' refers to the induction of biological effects in cells not directly targeted. The retinal pigment epithelium consists of hexagonal cells, forming a monolayer interconnected by gap junctions (GJs). Oxidative stress initiated in an individual cell by photostimulation (488 nm) triggered changes in reactive oxygen species (ROS), Ca(2+) and mitochondrial membrane potential (ψm). The Ca(2+) signal was transmitted to neighboring cells slowly and non-uniformly; the ROS signal spread fast and radially...
2017: Cell Death Discovery
https://www.readbyqxmd.com/read/28177904/genome-scale-analysis-identifies-gjb2-and-ero1lb-as-prognosis-markers-in-patients-with-pancreatic-cancer
#18
Tao Zhu, Yuan-Feng Gao, Yi-Xin Chen, Zhi-Bin Wang, Ji-Ye Yin, Xiao-Yuan Mao, Xi Li, Wei Zhang, Hong-Hao Zhou, Zhao-Qian Liu
Pancreatic cancer is a complex and heterogeneous disease with the etiology largely unknown. The deadly nature of pancreatic cancer, with an extremely low 5-year survival rate, renders urgent a better understanding of the molecular events underlying it. The aim of this study is to investigate the gene expression module of pancreatic adenocarcinoma and to identify differentially expressed genes (DEGs) with prognostic potentials. Transcriptome microarray data of five GEO datasets (GSE15471, GSE16515, GSE18670, GSE32676, GSE71989), including 117 primary tumor samples and 73 normal pancreatic tissue samples, were utilized to identify DEGs...
February 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28177159/connexin43-mutant-patient-derived-induced-pluripotent-stem-cells-exhibit-altered-differentiation-potential
#19
Jessica L Esseltine, Qing Shao, Courtney Brooks, Jacinda Sampson, Dean H Betts, Cheryle Séguin, Dale W Laird
We present for the first time the generation of induced pluripotent stem cells (iPSCs) from a patient with a connexin-linked disease. The importance of gap junctional intercellular communication in bone homeostasis is exemplified by the autosomal dominant developmental disorder oculodentodigital dysplasia (ODDD) which is linked to mutations in the GJA1 (Cx43) gene. ODDD is characterised by craniofacial malformations, ophthalmic deficits, enamel hypoplasia and syndactyly. In addition to harboring a Cx43 p.V216L mutation, ODDD iPSCs exhibit reduced Cx43 mRNA and protein abundance when compared to control iPSCs and display impaired channel function...
February 8, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28176351/role-of-connexin-43-in-cadmium-induced-proliferation-of-human-prostate-epithelial-cells
#20
Qingping Liu, Xiaoli Ji, Zehe Ge, Haipeng Diao, Xiuli Chang, Lihua Wang, Qing Wu
Connexins (Cxs), the subunits of gap junction channels, are involved in many physiological processes. Aberrant control of Cxs and gap junction intercellular communication may contribute to many diseases, including the promotion of cancer. Cd exposure is associated with increased risk of human prostate cancer and benign prostatic hyperplasia. The roles of Cxs in the effects of Cd on the prostate have, however, not been reported previously. In this study, the human prostate epithelial cell line RWPE-1 was exposed to Cd...
February 8, 2017: Journal of Applied Toxicology: JAT
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