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https://www.readbyqxmd.com/read/29332228/comparative-evaluation-of-the-aqueous-humor-proteome-of-primary-angle-closure-and-primary-open-angle-glaucomas-and-age-related-cataract-eyes
#1
Inderjeet Kaur, Jasbir Kaur, K Sooraj, Sandeep Goswami, Rohit Saxena, Virander Singh Chauhan, Ramanjit Sihota
PURPOSE: To analyze and compare the total proteome of aqueous humor (AH) from patients having primary angle closure glaucoma (PACG), primary open angle glaucoma (POAG) and age-related cataract. MATERIALS AND METHODOLOGY: Aqueous humor was collected from age-matched PACG, POAG and cataract patients who underwent surgery, and it was immediately stored at - 80 °C until analysis. From each sample, 25 µg of total protein was subjected to trypsin digestion and subsequently LC-MS/MS analysis was performed for the deep proteome analysis...
January 13, 2018: International Ophthalmology
https://www.readbyqxmd.com/read/29331002/genetics-of-human-hereditary-hearing-impairment
#2
Rahat Meena, Muhammad Ayub
Hereditary hearing impairment is heterogeneous type of disorder which can be caused due to environmental as well as genetical factors. Two distinct types of hereditary hearing loss are syndromic or non-syndromic. Non-syndromic hearing loss is further categorized as autosomal recessive, autosomal dominant, X-linked and mitochondrial deafness. Autosomal recessive occurs more frequently as compared to autosomal dominant. Mutations in various genes are responsible for hereditary hearing impairment. To date, about 99 autosomal recessives and 67 autosomal dominant genes for deafness have been discovered...
October 2017: Journal of Ayub Medical College, Abbottabad: JAMC
https://www.readbyqxmd.com/read/29327203/loss-of-cx43-mediated-functional-gap-junction-communication-in-meningeal-fibroblasts-following-mouse-hepatitis-virus-infection
#3
Abhishek Bose, Rahul Basu, Mahua Maulik, Jayasri Das Sarma
Mouse hepatitis virus (MHV) infection causes meningoencephalitis by disrupting the neuro-glial and glial-pial homeostasis. Recent studies suggest that MHV infection alters gap junction protein connexin 43 (Cx43)-mediated intercellular communication in brain and primary cultured astrocytes. In addition to astrocytes, meningeal fibroblasts also express high levels of Cx43. Fibroblasts in the meninges together with the basal lamina and the astrocyte endfeet forms the glial limitans superficialis as part of the blood-brain barrier (BBB)...
January 11, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29326832/at-the-atrioventricular-crossroads-dual-pathway-electrophysiology-in-the-atrioventricular-node-and-its-underlying-heterogeneities
#4
Sharon A George, N Rokhaya Faye, Alejandro Murillo-Berlioz, K Benjamin Lee, Gregory D Trachiotis, Igor R Efimov
The atrioventricular node (AVN) is a complex structure that performs a variety of functions in the heart. The AVN is primarily an electrical gatekeeper between the atria and ventricles and introduces a delay between atrial and ventricular excitation, allowing for efficient ventricular filling. The AVN is composed of several compartments that safely transmit electrical excitation from the atria to the ventricles via the fast or slow pathways. There are many electrophysiological differences between these pathways, including conduction time and electrical refractoriness, that increase the predisposition of the atrioventricular junction to arrhythmias such as atrioventricular nodal re-entrant tachycardia...
December 2017: Arrhythmia & Electrophysiology Review
https://www.readbyqxmd.com/read/29321356/the-connexin-46-mutant-v44m-impairs-gap-junction-function-causing-congenital-cataract
#5
Lijuan Chen, Dongmei Su, Sijia Li, Lina Guan, Cuige Shi, Dianjun Li, Shanshan Hu, Xu Ma
Connexin 46 (Cx46) is important for gap junction channels formation which plays crucial role in the preservation of lens homeostasis and transparency. Previously, we have identified a missense mutation (p.V44M) of Cx46 in a congenital cataract family. This study aims at dissecting the potential pathogenesis of the causative mutant of cataract. Plasmids carrying wild-type (wt) and mutant (V44M) of Cx46 were constructed and expressed in Hela cells respectively.Western blotting and fluorescence microscopy were applied to analyse the expression and subcellular localization of recombinant proteins, respectively...
December 2017: Journal of Genetics
https://www.readbyqxmd.com/read/29318123/genetic-linkage-analysis-of-dfnb4-dfnb28-dfnb93-loci-in-autosomal-recessive-non-syndromic-hearing-loss-evidence-for-digenic-inheritance-in-gjb2-and-gjb3-mutations
#6
Marzieh Naseri, Masoud Akbarzadehlaleh, Marjan Masoudi, Najmeh Ahangari, Ali Akbar Poursadegh Zonouzi, Ahmad Poursadegh Zonouzi, Leila Shams, Azim Nejatizadeh
Background: Autosomal recessive non-syndromic hearing loss (ARNSHL) a most frequent hereditary type of hearing impairment, exhibit tremendous genetic heterogeneity. We aimed to determine the contribution of three common DFNB loci (DFNB4, DFNB28, and DFNB93), and mutation analysis of Gap Junction Beta-2 gene (GJB2) and GJB3 genes in ARNSHL subjects in southern Iran. Methods: Thirty-six large ARNSHL pedigrees (167 individuals) with at least two affected subjects (72 patients) were included in this descriptive study from Hormozgan Province of Iran, during 2014 - 2015...
January 2018: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/29317708/connexin-43-hemichannel-as-a-novel-mediator-of-sterile-and-infectious-inflammatory-diseases
#7
Wei Li, Guoqiang Bao, Weiqiang Chen, Xiaoling Qiang, Shu Zhu, Shuaiwei Wang, Mingzhu He, Gaifeng Ma, Mahendar Ochani, Yousef Al-Abed, Huan Yang, Kevin J Tracey, Ping Wang, John D'Angelo, Haichao Wang
Cytoplasmic membrane-bound connexin 43 (Cx43) proteins oligomerize into hexameric channels (hemichannels) that can sometimes dock with hemichannels on adjacent cells to form gap junctional (GJ) channels. However, the possible role of Cx43 hemichannels in sterile and infectious inflammatory diseases has not been adequately defined due to the lack of selective interventions. Here we report that a proinflammatory mediator, the serum amyloid A (SAA), resembled bacterial endotoxin by stimulating macrophages to up-regulate Cx43 expression and double-stranded RNA-activated protein kinase R (PKR) phosphorylation in a TLR4-dependent fashion...
January 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29315933/an-alkylated-indacenodithieno-3-2-b-thiophene-based-nonfullerene-acceptor-with-high-crystallinity-exhibiting-single-junction-solar-cell-efficiencies-greater-than-13-with-low-voltage-losses
#8
Zhuping Fei, Flurin D Eisner, Xuechen Jiao, Mohammed Azzouzi, Jason A Röhr, Yang Han, Munazza Shahid, Anthony S R Chesman, Christopher D Easton, Christopher R McNeill, Thomas D Anthopoulos, Jenny Nelson, Martin Heeney
A new synthetic route, to prepare an alkylated indacenodithieno[3,2-b]thiophene-based nonfullerene acceptor (C8-ITIC), is reported. Compared to the reported ITIC with phenylalkyl side chains, the new acceptor C8-ITIC exhibits a reduction in the optical band gap, higher absorptivity, and an increased propensity to crystallize. Accordingly, blends with the donor polymer PBDB-T exhibit a power conversion efficiency (PCE) up to 12.4%. Further improvements in efficiency are found upon backbone fluorination of the donor polymer to afford the novel material PFBDB-T...
January 9, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29315235/response-of-fibroblasts-mrc-5-to-flufenamic-acid-grafted-mcm-41-nanoparticles
#9
Giovanna Gomes Lara, Marcelo Fernandes Cipreste, Gracielle Ferreira Andrade, Wellington Marcos da Silva, Edésia Martins Barros de Sousa
Recently, flufenamic acid (FFA) was discovered among fenamates as a free radical scavenger and gap junction blocker; however, its effects have only been studied in cancer cells. Normal cells in the surroundings of a tumor also respond to radiation, although they are not hit by it directly. This phenomenon is known as the bystander effect, where response molecules pass from tumor cells to normal ones, through communication channels called gap junctions. The use of the enhanced permeability and retention effect, through which drug-loaded nanoparticles smaller than 200 nm may accumulate around a tumor, can prevent the local side effect upon controlled release of the drug...
January 9, 2018: Bioengineering
https://www.readbyqxmd.com/read/29314148/3d-reconstruction-of-the-porcine-and-equine-pulmonary-veins-supplemented-with-the-identification-of-telocytes-in-the-horse
#10
T Vandecasteele, W Van Den Broeck, H Tay, L Couck, G van Loon, P Cornillie
The myocardial sleeve of the porcine and equine pulmonary veins were histologically investigated and reconstructed three dimensionally. Moreover, the localization of neuron cell bodies at the veno-atrial junction and alongside the myocardial sleeve was light microscopically visualized to depict the organization of nerve, myocardial and fat tissue. Finally, the presence of telocytes inside the equine pulmonary veins was demonstrated by use of transmission electron microscopy. These structures are thought to play a role in the induction of atrial fibrillation, which is frequently seen in horses, while pigs are often used as a cardiovascular model in this context...
January 3, 2018: Anatomia, Histologia, Embryologia
https://www.readbyqxmd.com/read/29313035/peptide-delivery-with-poly-ethylene-glycol-diacrylate-microneedles-through-swelling-effect
#11
Shiying Liu, David C Yeo, Christian Wiraja, Hong Liang Tey, Milan Mrksich, Chenjie Xu
Transdermal delivery of therapeutic biomolecules (including peptides) can avoid enzymatic digestion that occurs in the oral route. (Polyethylene glycol) diacrylate (PEGDA)-based microneedles, with good biocompatibility, are easily fabricated through photo-polymerization with a precisely controlled structure. It has successfully been used for the transdermal delivery of small molecule drugs such as 5-fluorouracil. However, the delivery of peptide-based therapeutics using this platform is seldom reported. This is because of the potential damage to the peptide during the photo-polymerization process of PEGDA...
September 2017: Bioengineering & Translational Medicine
https://www.readbyqxmd.com/read/29312613/dysregulated-connexin-43-in-her2-positive-drug-resistant-breast-cancer-cells-enhances-proliferation-and-migration
#12
Elizabeth S Yeh, Christina J Williams, Carly Bess Williams, Ingrid V Bonilla, Nancy Klauber-DeMore, Stephanie L Phillips
Connexin 43 (Cx43) is a gap junction protein whose function in the development of breast cancer and in breast cancer progression remains unclear. Evidence suggests that Cx43 (GJA1) mRNA and protein expression is altered in breast tumors. However, reports indicate both increased and decreased Cx43 levels in human breast cancer samples. Studies also suggest that loss of Cx43 regulated gap junction intercellular communication is a common feature of breast malignancies that potentially correlates with histological stage...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29308765/-mitochondrial-transfer-mechanism-of-stem-cells-for-therapy-of-lung-injury
#13
Jinwang Han, Yanling Song, Feng Zhu
Stem cells transfer normal functional mitochondria to injured lung cells and reconstruct their function, which is one of the important mechanisms of stem cells in the treatment of lung injury. Mitochondrial translocation is mediated by intercellular gap junction (GJCs), which is unidirectional. This paper reviewed the research of stem cells for the treatment of lung injury mitochondrial transfer mechanism, to understand the clinical significance of lung mitochondria in physiological and pathological roles of mitochondria and transfer mechanisms of stem cells for the treatment of various types of lung injury...
January 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29305924/mechanistic-understanding-the-bioeffects-of-ultrasound-driven-microbubbles-to-enhance-macromolecule-delivery
#14
REVIEW
Peng Qin, Tao Han, Alfred C H Yu, Lin Xu
Ultrasound-driven microbubbles can trigger reversible membrane perforation (sonoporation), open interendothelial junctions and stimulate endocytosis, thereby providing a temporary and reversible time-window for the delivery of macromolecules across biological membranes and endothelial barriers. This time-window is related not only to cavitation events, but also to biological regulatory mechanisms. Mechanistic understanding of the interaction between cavitation events and cells and tissues, as well as the subsequent cellular and molecular responses will lead to new design strategies with improved efficacy and minimized side effects...
January 3, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29303486/hearts-during-ischemia-with-or-without-htk-protection-analysed-by-dielectric-spectroscopy
#15
Michael Schaefer, Wolfgang Gross, Martha-Maria Gebhard
We investigated canine hearts during ischemia after aortic cross clamping (UI, n=20) and after HTK-cardioplegia (HTK, n=24) at 35°C, 25°C, 15°C, and 5°C with the aim to compare tissue changes caused by the activity of anaerobic metabolism(AAM), cell membrane destruction(CD), and gap junction uncoupling(GJU). We measured continuously the complex dielectric spectrum(DS), ATP- and lactate content, extracellular pH, and rigor contracture. To identify changes in DS caused by AAM, CD, and GJU we performed additional experiments on the gap junction-free skeletal muscle...
January 5, 2018: Physiological Measurement
https://www.readbyqxmd.com/read/29301784/trpv4-transient-receptor-potential-vanilloid-4-channel-dependent-negative-feedback-mechanism-regulates-gq-protein-coupled-receptor-induced-vasoconstriction
#16
Kwangseok Hong, Eric L Cope, Leon J DeLalio, Corina Marziano, Brant E Isakson, Swapnil K Sonkusare
OBJECTIVE: Several physiological stimuli activate smooth muscle cell (SMC) GqPCRs (Gq protein-coupled receptors) to cause vasoconstriction. As a protective mechanism against excessive vasoconstriction, SMC GqPCR stimulation invokes endothelial cell vasodilatory signaling. Whether Ca2+ influx in endothelial cells contributes to the regulation of GqPCR-induced vasoconstriction remains unknown. Ca2+ influx through TRPV4 (transient receptor potential vanilloid 4) channels is a key regulator of endothelium-dependent vasodilation...
January 4, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29298877/molecular-basis-for-potentiation-of-cx36-gap-junction-channel-conductance-by-n-alcohols-and-general-anesthetics
#17
Vytautas Raškevičius, Vaidas Jotautis, Lina Rimkutė, Alina Marandykina, Mintautė Kazokaitė, Visvaldas Kairys, Vytenis Arvydas Skeberdis
In our recent study, we have demonstrated that short carbon chain n -alcohols (up to octanol) stimulated while long carbon chain n -alcohols inhibited the conductance of connexin 36 (Cx36) gap junction (GJ) channels. In contrast, GJ channels composed of other types of Cxs all were inhibited by n -alcohols independently on their carbon chain length. To identify the putative structural domains of Cx36, responsible for the dual effect of n -alcohols, we performed structural modeling of Cx36 protein docking with hexanol and isoflurane that stimulated as well as nonanol and carbenoxolone that inhibited the conductance of Cx36 GJs and revealed their multiple common docking sites and a single pocket accessible only to hexanol and isoflurane...
January 3, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29297522/one-dimensional-cadmium-sulphide-nanotubes-for-photocatalytic-water-splitting
#18
Lin Ju, Ying Dai, Wei Wei, Mengmeng Li, Yan Liang, Baibiao Huang
Recently, a two-dimensional (2D) stable CdS monolayer with remarkable visible-light photocatalytic activity for water splitting has been proposed via theoretical calculations. It is also interesting to explore the photocatalytic activity of one-dimensional (1D) CdS nanotubes (CSNTs). In the present study, we have designed atomic models for CSNTs in the 5.2-19.2 Å outer diameter range and investigated the basic properties using density functional theory. It is shown that the CSNTs have negative strain energies when the diameter is beyond 6...
January 3, 2018: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29295866/connexin-43-gap-junctions-contribute-to-brain-endothelial-barrier-hyperpermeability-in-familial-cerebral-cavernous-malformations-type-iii-by-modulating-tight-junction-structure
#19
Allison M Johnson, James P Roach, Anna Hu, Svetlana M Stamatovic, Michal R Zochowski, Richard F Keep, Anuska V Andjelkovic
Familial cerebral cavernous malformations type III (fCCM3) is a disease of the cerebrovascular system caused by loss-of-function mutations in ccm3 that result in dilated capillary beds that are susceptible to hemorrhage. Before hemorrhage, fCCM3 lesions are characterized by a hyperpermeable blood-brain barrier (BBB), the key pathologic feature of fCCM3. We demonstrate that connexin 43 (Cx43), a gap junction (GJ) protein that is incorporated into the BBB junction complex, is up-regulated in lesions of a murine model of fCCM3...
January 2, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29290021/dl-3-n-butylphthalide-reduces-atrial-fibrillation-susceptibility-by-inhibiting-atrial-structural-remodeling-in-rats-with-heart-failure
#20
Huiliang Qiu, Huanlin Wu, Jin Ma, Haiming Cao, Lihua Huang, Wencong Qiu, Ying Peng, Chunhua Ding
Agents against atrial structural remodeling (ASR) are thought to block the occurrence of atrial fibrillation (AF). The aim of this study was to investigate the effects of DL-3-n-butylphthalide (NBP) on ASR and AF formation in rats with heart failure (HF) induced by myocardial infarction. The heart failure rats established 1 week after ligating left anterior descending coronary artery were randomly treated with vehicle (HF group, n = 24), or treated with DL-3-n-butylphthalide (100 mg/kg body weight) (NBP group, n = 26) for 4 weeks...
December 30, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
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