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https://www.readbyqxmd.com/read/29642568/irradiation-induced-cardiac-connexin-43-and-mir-21-responses-are-hampered-by-treatment-with-atorvastatin-and-aspirin
#1
Csilla Viczenczova, Branislav Kura, Tamara Egan Benova, Chang Yin, Rakesh C Kukreja, Jan Slezak, Narcis Tribulova, Barbara Szeiffova Bacova
Radiation of the chest during cancer therapy is deleterious to the heart, mostly due to oxidative stress and inflammation related injury. A single sub-lethal dose of irradiation has been shown to result in compensatory up-regulation of the myocardial connexin-43 (Cx43), activation of the protein kinase C (PKC) signaling along with the decline of microRNA (miR)-1 and an increase of miR-21 levels in the left ventricle (LV). We investigated whether drugs with antioxidant, anti-inflammatory or vasodilating properties, such as aspirin, atorvastatin, and sildenafil, may affect myocardial response in the LV and right ventricle (RV) following chest irradiation...
April 10, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29627440/cyclic-biaxial-tensile-strain-promotes-bone-marrow-derived-mesenchymal-stem-cells-to-differentiate-into-cardiomyocyte-like-cells-by-mirna-27a
#2
Chengjian Cao, Liang Li, Huiming Li, Xueling He, Wu Geng, Yu Xiaoqin
A physical stimuli, it has been reported that cyclic tensile strain can promote bone marrow-derived mesenchymal stem cells (BMSCs) to differentiate into cardiomyocytes, but the underlying mechanisms have been poorly elucidated so far. Here, we used a mimicking loading strain, cyclic biaxial tensile strain (CBTS), and found it can promote BMSCs to differentiate into cardiomyocytes. When the CBTS were loaded, the cells expressed cardiac-specific markers GATA4, TNNT2, MEF-2c, and Cx43, meanwhile we found miR-27a decreased and stem cell factor (SCF) increased...
April 5, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29626091/the-ubiquitin-specific-protease-usp8-deubiquitinates-and-stabilizes-cx43
#3
Jian Sun, Qianwen Hu, Hong Peng, Cheng Peng, Liheng Zhou, Jinsong Lu, Chuanxin Huang
Connexin 43 (Cx43, also known as GJA1), is the most ubiquitously expressed connexin isoform in mammalian tissues. It forms intercellular gap junction (GJ) channels, enabling adjacent cells to communicate both electrically and metabolically. Cx43 is a short-lived protein which can be quickly degraded by the ubiquitin-dependent proteasomal, endolysosomal and autophagosomal pathways. Here, we report that the ubiquitin-specific peptidase 8 (USP8) interacts with and deubiquitinates Cx43. USP8 reduces both multiple monoubiquitination and polyubiquitination of Cx43 to prevent autophagy-mediated degradation...
April 6, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29623602/inhibition-of-astrocyte-connexin-43-channels-facilitates-the-differentiation-of-oligodendrocyte-precursor-cells-under-hypoxic-conditions-in-vitro
#4
Qiong Wang, Zhen Wang, Yeye Tian, Huaqiu Zhang, Yongkang Fang, Zhiyuan Yu, Wei Wang, Minjie Xie, Fengfei Ding
Oligodendrocyte precursor cells (OPCs) proliferation and differentiation are essential for remyelination after white matter injury. Astrocytes could promote oligodendrogenesis after white matter damage whereas the underlying mechanisms are unknown. In this study, the role of astrocytic connexin43 (Cx43) hemichannels involved in OPC proliferation and differentiation in chronic hypoxia was evaluated. In an astrocyte-OPC co-culture chronic hypoxia model, OPCs became proliferative but failed to mature into oligodendrocytes...
April 5, 2018: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/29618634/mice-harbouring-an-oculodentodigital-dysplasia-linked-cx43-g60s-mutation-have-severe-hearing-loss
#5
Julia M Abitbol, John J Kelly, Kevin J Barr, Brian L Allman, Dale W Laird
Given the importance of connexin43 (Cx43) function in the central nervous system and sensory organ processing we proposed that it would also be crucial in auditory function. To that end, hearing was examined in two mouse models of oculodentodigital dysplasia that globally express GJA1 (Cx43) mutations resulting in mild or severe loss of Cx43 function. Although Cx43I130T/+ mutant mice with ∼50% Cx43 channel function did not have any hearing loss, Cx43G60S/+ mutant mice with ∼20% Cx43 channel function had severe hearing loss...
April 3, 2018: Journal of Cell Science
https://www.readbyqxmd.com/read/29602954/dl-3-n-butylphthalide-improves-ventricular-function-and-prevents-ventricular-remodeling-and-arrhythmias-in-post-mi-rats
#6
Huiliang Qiu, Jin Ma, Huanlin Wu, Chunhua Ding
DL-3-n-butylphthalide (NBP) is used in the treatment of ischemic stroke. It was demonstrated NBP also has a cardioprotective effect in acute myocardial infarction (MI) model. However, the chronic effects of NBP on ventricular function, remodeling, and arrhythmias in post-MI stage are unknown. This study was to investigate the effect of NBP on reducing ventricular remodeling and arrhythmias in post-MI stage. Post-MI rats were randomly treated with 100 mg/kg NBP daily (n = 21) or vehicle (n = 21) for 5 weeks...
March 30, 2018: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/29600160/multifaceted-roles-of-connexin-43-in-stem-cell-niches
#7
REVIEW
Nafiisha Genet, Neha Bhatt, Antonin Bourdieu, Karen K Hirschi
Purpose of Review: Considerable progress has been made in the field of stem cell research; nonetheless, the use of stem cells for regenerative medicine therapies, for either endogenous tissue repair or cellular grafts post injury, remains a challenge. To better understand how to maintain stem cell potential in vivo and promote differentiation ex vivo, it is fundamentally important to elucidate the interactions between stem cells and their surrounding partners within their distinct niches...
2018: Current Stem Cell Reports
https://www.readbyqxmd.com/read/29597134/hyaluronic-acid-coated-albumin-nanoparticles-for-targeted-peptide-delivery-in-the-treatment-of-retinal-ischaemia
#8
Di Huang, Ying-Shan Chen, Colin R Green, Ilva D Rupenthal
Recent studies have shown that Connexin43 mimetic peptide (Cx43 MP) can prevent secondary damage following retinal ischaemic and inflammatory disorders by blocking uncontrolled Cx43 hemichannel opening. However, limitations in peptide stability and the presence of various intraocular barriers limit efficient retinal delivery in the clinical setting. The present study aimed to achieve targeted and sustained peptide delivery to the retina by encapsulating Cx43 MP into hyaluronic acid (HA) coated albumin nanoparticles (NPs)...
March 20, 2018: Biomaterials
https://www.readbyqxmd.com/read/29596891/connexin-43-regulates-the-expression-of-wound-healing-related-genes-in-human-gingival-and-skin-fibroblasts
#9
Rana Tarzemany, Guoqiao Jiang, Jean X Jiang, Corrie Gallant-Behm, Colin Wiebe, David A Hart, Hannu Larjava, Lari Häkkinen
Fibroblasts are the most abundant connective tissue cells and play an important role in wound healing. It is possible that faster and scarless wound healing in oral mucosal gingiva relative to skin may relate to the distinct phenotype of the fibroblasts residing in these tissues. Connexin 43 (Cx43) is the most ubiquitous Cx in skin (SFBLs) and gingival fibroblasts (GFBLs), and Cx43 assembles into hemichannels (HCs) and gap junctions (GJs) on the cell membrane. We hypothesized that SFBLs and GFBLs display distinct expression or function of Cx43, and that this may partly underlie the different wound healing outcomes in skin and gingiva...
March 26, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29587860/roles-of-astrocytic-connexin-43-hemichannels-and-gap-junctions-in-oxygen-glucose-deprivation-reperfusion-injury-induced-neuroinflammation-and-the-possible-regulatory-mechanisms-of-salvianolic-acid-b-and-carbenoxolone
#10
Xiang Yin, Liangshu Feng, Di Ma, Ping Yin, Xinyu Wang, Shuai Hou, Yulei Hao, Jingdian Zhang, Meiying Xin, Jiachun Feng
BACKGROUND: Glia-mediated neuroinflammation is related to brain injury exacerbation after cerebral ischemia/reperfusion (I/R) injury. Astrocytic hemichannels or gap junctions, which were mainly formed by connexin-43, have been implicated in I/R damage. However, the exact roles of astrocytic hemichannels and gap junction in neuroinflammatory responses induced by I/R injury remain unknown. METHODS: Primary cultured astrocytes were subjected to OGD/R injury, an in vitro model of I/R injury...
March 27, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29587382/functional-characterization-of-novel-atrial-fibrillation-linked-gja5-cx40-mutants
#11
Mahmoud Noureldin, Honghong Chen, Donglin Bai
Atrial fibrillation (AF) is the most common form of cardiac arrhythmia. Recently, four novel heterozygous Cx40 mutations-K107R, L223M, Q236H, and I257L-were identified in 4 of 310 unrelated AF patients and a followup genetic analysis of the mutant carriers' families showed that the mutants were present in all the affected members. To study possible alterations associated with these Cx40 mutants, including their cellular localization and gap junction (GJ) function, we expressed GFP-tagged and untagged mutants in connexin-deficient model cells...
March 25, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29587265/transforming-growth-factor-beta-1-drives-a-switch-in-connexin-mediated-cell-to-cell-communication-in-tubular-cells-of-the-diabetic-kidney
#12
Claire Hills, Gareth William Price, Mark John Wall, Timothy John Kaufmann, Sidney Chi-Wai Tang, Wai Han Yiu, Paul Edward Squires
BACKGROUND/AIMS: Changes in cell-to-cell communication have been linked to several secondary complications of diabetes, but the mechanism by which connexins affect disease progression in the kidney is poorly understood. This study examines a role for glucose-evoked changes in the beta1 isoform of transforming growth factor (TGFβ1), on connexin expression, gap-junction mediated intercellular communication (GJIC) and hemi-channel ATP release from tubular epithelial cells of the proximal renal nephron...
March 26, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29578247/benzo-a-pyrene-decreased-gap-junctional-intercellular-communication-via-calcium-calmodulin-signaling-increases-apoptosis-in-tm4-cells
#13
Xiaoli Ji, Xin Chou, Zehe Ge, Fan Ding, Hui Gao, Qing Wu
The mechanism of male reproductive toxicity induced by benzo[a]pyrene (BaP) is poorly understood. Gap junctional intercellular communication (GJIC) is known to play a critical role in maintaining spermatogenesis. The aim of the present study was to determine the toxic effects of BaP in Sertoli cells, and to explore the possibility and potential mechanisms of BaP-induced changes in the level of GJIC, and the relationship between GJIC and BaP-induced apoptosis. We treated mouse Sertoli cell lines (TM4) with different concentrations (0...
March 26, 2018: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/29577900/protective-effects-of-kenpaullone-on-cardiomyocytes-following-h-2-o-2-induced-oxidative-stress-are-attributed-to-inhibition-of-connexin-43-degradation-by-sgsm3
#14
Hyun-Chel Joo, Jung-Won Choi, Hanbyeol Moon, Chang Youn Lee, Kyung-Jong Yoo, Sang Woo Kim, Ki-Chul Hwang
A previous study showed that small G protein signaling modulator 3 (SGSM3) was highly correlated with Cx43 in heart functions and that high levels of SGSM3 may induce Cx43 turnover through lysosomal degradation in infarcted rat hearts. Here, we investigated the protective effects of kenpaullone on cardiomyocytes following H2 O2 -induced oxidative stress mediated by the interaction of SGSM3 with Cx43. We found that the gap junction protein Cx43 was significantly down-regulated in an H2 O2 concentration-dependent manner, whereas expression of SGSM3 was up-regulated upon H2 O2 exposure in H9c2 cells...
March 22, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29571253/in-vitro-inhibited-effect-of-gap-junction-composed-of-cx43-in-the-invasion-and-metastasis-of-testicular-cancer-resistanced-to-cisplatin
#15
Dandan Wu, Beibei Li, Haofeng Liu, Min Yuan, Meiling Yu, Liang Tao, Shuying Dong, Xuhui Tong
The effect of gap junction intercellular communication composed of connexin on cancer invasion/metastasis has been thoroughly explored; however, its effect on testicular cancer resistanced to chemotherapy is still unclear. In this study, we found that the capability of invasion and migration of I-10/DDP (cisplatin (DDP)-resistance) cells were elevated. Furthermore, the expression of Cx43 and the function of gap junction (GJ) in I-10/DDP cells were decreased compared with parental I-10 cells. Pharmacological inhibition of GJs by oleamide (Olea) enhanced invasion and migration...
February 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29570626/angiotensin-ii-induced-mesangial-cell-damaged-is-preceded-by-cell-membrane-permeabilization-due-to-upregulation-of-non-selective-channels
#16
Gonzalo I Gómez, Paola Fernández, Victoria Velarde, Juan C Sáez
Connexin43 (Cx43), pannexin1 (Panx1) and P2X₇ receptor (P2X₇R) are expressed in kidneys and are known to constitute a feedforward mechanism leading to inflammation in other tissues. However, the possible functional relationship between these membrane channels and their role in damaged renal cells remain unknown. In the present work, we found that MES-13 cells, from a cell line derived from mesangial cells, stimulated with angiotensin II (AngII) developed oxidative stress (OS, thiobarbituric acid reactive species (TBARS) and generated pro-inflammatory cytokines (ELISA; IL-1β and TNF-α)...
March 23, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29570224/a-rare-missense-mutation-in-gjb3-cx31g45e-is-associated-with-a-unique-cellular-phenotype-resulting-in-necrotic-cell-death
#17
J A Easton, A K Alboulshi, M A F Kamps, G H Brouns, M R Broers, B J Coull, V Oji, M van Geel, M A M van Steensel, P E Martin
Erythrokeratodermia variabilis et progressiva (EKV-P) is caused by mutations in either the GJB3 (Cx31) or GJB4 gene (Cx30.3). We identified a rare GJB3 missense mutation, c.134G>A (p.G45E), in two unrelated patients and investigated its cellular characteristics. Expression of Cx31G45E-GFP caused previously undescribed changes within HeLa cells and HaCaT cells, a model human keratinocyte cell line. Cx31WT-GFP localised to the plasma membrane, but expression of Cx31G45E-GFP caused vacuolar expansion of the endoplasmic reticulum (ER), the mutant protein accumulated within the ER membrane and disassembly of the microtubular network occurred...
March 23, 2018: Experimental Dermatology
https://www.readbyqxmd.com/read/29565275/connexin-43-controls-the-astrocyte-immunoregulatory-phenotype
#18
Anne-Cécile Boulay, Alice Gilbert, Vanessa Oliveira Moreira, Corinne Blugeon, Sandrine Perrin, Juliette Pouch, Stéphane Le Crom, Bertrand Ducos, Martine Cohen-Salmon
Astrocytes are the most abundant glial cells of the central nervous system and have recently been recognized as crucial in the regulation of brain immunity. In most neuropathological conditions, astrocytes are prone to a radical phenotypical change called reactivity, which plays a key role in astrocyte contribution to neuroinflammation. However, how astrocytes regulate brain immunity in healthy conditions is an understudied question. One of the astroglial molecule involved in these regulations might be Connexin 43 (Cx43), a gap junction protein highly enriched in astrocyte perivascular endfeet-terminated processes forming the glia limitans...
March 22, 2018: Brain Sciences
https://www.readbyqxmd.com/read/29555979/helium-alters-the-cytoskeleton-and-decreases-permeability-in-endothelial-cells-cultured-in-vitro-through-a-pathway-involving-caveolin-1
#19
Kirsten F Smit, Moritz Konkel, Raphaela Kerindongo, Maximilian A Landau, Coert J Zuurbier, Markus W Hollmann, Benedikt Preckel, Rienk Nieuwland, Martin Albrecht, Nina C Weber
Caveolins are involved in anaesthetic-induced cardioprotection. Actin filaments are located in close connection to Caveolins in the plasma membrane. We hypothesised that helium might affect the cytoskeleton and induce secretion of Caveolin. HCAEC, HUVEC and Cav-1 siRNA transfected HUVEC were exposed for 20 minutes to either helium (5% CO2 , 25% O2 , 70% He) or control gas (5% CO2 , 25% O2 , 70% N2 ). Cells and supernatants were collected for infrared Western blot analysis, immunofluorescence staining, nanoparticle tracking analysis and permeability measurements...
March 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29551657/aspirin-inhibits-growth-and-enhances-cardiomyocyte-differentiation-of-bone-marrow-mesenchymal-stem-cells
#20
Wen Hao, Shutian Shi, Shenghui Zhou, Xiao Wang, Shaoping Nie
This study aimed to examine the effects of aspirin on the growth and cardiomyocyte differentiation grade of bone marrow mesenchymal stem cells (BMMSCs). BMMSCs were divided into five differentiation groups with different concentrations of aspirin (0mM, 0.5mM, 1mM, 2mM, or 5mM), and a undifferentiated control group. Cell growth was measured by cell proliferation, apoptosis assays and DNA cycle analysis. The differentiation grade of BMMSC-derived cardiomyocyte-like cells was examined by measuring the levels of cardiac-specific proteins with cyto-immunofluorescence staining, flow cytometry, and Western blotting...
March 15, 2018: European Journal of Pharmacology
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