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https://www.readbyqxmd.com/read/29238079/dorsal-root-ganglion-neurons-regulate-the-transcriptional-and-translational-programs-of-osteoblast-differentiation-in-a-microfluidic-platform
#1
Diana Isabel Silva, Bruno Paiva Dos Santos, Jacques Leng, Hugo Oliveira, Joëlle Amédée
Innervation by the sensory nervous system plays a key role in skeletal development and in orchestration of bone remodeling and regeneration. However, it is unclear how and in which bone cells can sensory nerves act to control these processes. Here, we show a microfluidic coculture system comprising dorsal root ganglion (DRG) neurons and mesenchymal stem cells (MSCs) that more faithfully represents the in vivo scenario of bone sensory innervation. We report that DRG neurons promote the osteogenic differentiation capacity of MSCs, by mediating the increase of alkaline phosphatase activity and the upregulation of osteoblast-specific genes...
December 13, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29235124/silver-nanoparticles-increase-connexin43-mediated-gap-junctional-intercellular-communication-in-hacat-cells-through-activation-of-reactive-oxygen-species-and-mitogen-activated-protein-kinase-signal-pathway
#2
Yu Qin, Limin Han, Di Yang, Hongying Wei, Yue Liu, Junhui Xu, Herman Autrup, Furong Deng, Xinbiao Guo
Silver nanoparticles (AgNPs) are widely used in health and consumer products that routinely contact skin. However, the biological effects and possible mechanisms of AgNPs on skin remain unclear. Gap junctional intercellular communication (GJIC) plays a critical role in multicellular organisms to maintain tissue homeostasis. The aim of this study is to examine if non-coated AgNPs affect GJIC in human keratinocytes (HaCaT cells), and to identify the possible molecular mechanisms responsible for the effects. GJIC, connexin (Cx)43 protein and mRNA expression, and the effect of siRNA-mediated knockdown of Cx43 on GJIC were assessed...
December 13, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/29234131/a-gene-expression-study-of-ornamental-fin-shape-in-neolamprologus-brichardi-an-african-cichlid-species
#3
Ehsan Pashay Ahi, Florian Richter, Kristina M Sefc
The diversity of fin morphology within and across fish taxa offers great, but still largely unexplored, opportunities to investigate the proximate mechanisms underlying fin shape variation. Relying on available genetic knowledge brought forth mainly by the comprehensive study of the zebrafish caudal fin, we explored candidate molecular mechanisms for the maintenance and formation of the conspicuously elongated filaments adorning the unpaired fins of the East African "princess cichlid" Neolamprologus brichardi...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29228446/telmisartan-reduces-arrhythmias-through-increasing-cardiac-connexin43-by-inhibiting-il-17-after-myocardial-infarction-in-rats
#4
H-Y Chang, X Li, Y Tian
OBJECTIVE: To observe the expressions of myocardial connexin43 (Cx43) and interleukin-17 (IL-17) in acute myocardial infarction (AMI) rats and investigate its possible mechanism of telmisartan in the prevention and treatment of arrhythmia in AMI. MATERIALS AND METHODS: Sprague Dawley (SD) rats were selected and myocardial infarction model was established. After the successful modeling, the rats were randomly divided into three groups: Sham group, MI group, Telm group...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29224922/neurons-and-satellite-glial-cells-in-adult-rat-lumbar-dorsal-root-ganglia-express-connexin-36
#5
E Martha Pérez Armendariz, Monica Norcini, Beatriz Hernández-Tellez, Andrés Castell-Rodríguez, Cristina Coronel-Cruz, Raquel Guerrero Alquicira, Alexandra Sideris, Esperanza Recio-Pinto
Previous studies have shown that following peripheral nerve injury there was a downregulation of the gap junction protein connexin 36 (Cx36) in the spinal cord; however, it is not known whether Cx36 protein is expressed in the dorsal root ganglia (DRGs), nor if its levels are altered following peripheral nerve injuries. Here we address these aspects in the adult rat lumbar DRG. Cx36 mRNA was detected using qRT-PCR, and Cx36 protein was identified in DRG sections using immunohistochemistry (IHC) and immunofluorescence (IF)...
December 7, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/29223529/corticosterone-impairs-gap-junctions-in-the-prefrontal-cortical-and-hippocampal-astrocytes-via-different-mechanisms
#6
Cong-Yuan Xia, Zhen-Zhen Wang, Zhao Zhang, Jiao Chen, Ying-Ying Wang, Yu-Xia Lou, Yan Gao, Piao Luo, Qian Ren, Guo-Hua Du, Nai-Hong Chen
Increasing evidence has implicated astrocyte pathology in the etiopathology of major depressive disorder (MDD). In particular, dysfunction of gap junctions in astrocytes is a potential target for MDD treatment. However, the mechanism underlying stress-induced dysfunction of gap junctions is still unknown. We therefore studied the mechanism of stress-induced dysfunction of gap junctions in prefrontal cortical and hippocampal astrocytes. Corticosterone (CORT) was used to induce stress conditions; CORT damaged the function of gap junctions, which resulted from less distribution of connexin43 (Cx43) on membranes and the enhanced phosphorylation of Cx43 at S368...
December 6, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/29218600/the-sh3-binding-domain-of-cx43-participates-in-loop-tail-interactions-critical-for-cx43-hemichannel-activity
#7
Jegan Iyyathurai, Nan Wang, Catheleyne D'hondt, Jean X Jiang, Luc Leybaert, Geert Bultynck
Connexin 43 (Cx43) hemichannels establish local signaling networks via the release of ATP and other molecules, but their excessive opening may result in cell death. Hence, the activity of Cx43-hemichannels ought to be critically controlled. This involves interactions between the C-terminal tail (CT) and the cytoplasmic loop (CL), more particularly the L2 domain within CL. Previous work revealed an important role for the last nine amino acids of the Cx43 CT by targeting the L2 domain, as these nine amino acids were sufficient to restore the activity of CT-truncated Cx43-hemichannels...
December 7, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29212255/soluble-epoxide-hydrolase-inhibitors-t-aucb-regulated-microrna-1-and-its-target-genes-in-myocardial-infarction-mice
#8
Ya-Jun Gui, Tao Yang, Qiong Liu, Cai-Xiu Liao, Jing-Yuan Chen, Ya-Ting Wang, Jia-Hui Hu, Dan-Yan Xu
Purpose: Soluble epoxide hydrolase inhibitors (sEHIs) had been demonstrated to produce cardioprotective effects against ischemia-induced lethal arrhythmias, but the exact mechanisms remain unknown. The present study was designed to investigate whether the beneficial effects of sEHIs are related to regulation of microRNA-1, which was a proarrhythmic factor in the ischemic heart. Methods: A mousemyocardial infarction (MI) model was established by ligating the coronary artery...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29209227/the-role-of-na-k-2cl-cotransporter-1-nkcc1-slc12a2-in-dental-epithelium-during-enamel-formation-in-mice
#9
Rozita Jalali, Johannes C Lodder, Behrouz Zandieh-Doulabi, Dimitra Micha, James E Melvin, Marcelo A Catalan, Huibert D Mansvelder, Pamela DenBesten, Antonius Bronckers
Na+:K+:2Cl- cotransporters (NKCCs) belong to the SLC12A family of cation-coupled Cl- transporters. We investigated whether enamel-producing mouse ameloblasts express NKCCs. Transcripts for Nkcc1 were identified in the mouse dental epithelium by RT-qPCR and NKCC1 protein was immunolocalized in outer enamel epithelium and in the papillary layer but not the ameloblast layer. In incisors of Nkcc1-null mice late maturation ameloblasts were disorganized, shorter and the mineral density of the enamel was reduced by 10% compared to wild-type controls...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29200997/connexin43-hemichannels-in-satellite-glial-cells-can-they-influence-sensory-neuron-activity
#10
REVIEW
Mauricio A Retamal, Manuel A Riquelme, Jimmy Stehberg, Julio Alcayaga
In this review article, we summarize the current insight on the role of Connexin- and Pannexin-based channels as modulators of sensory neurons. The somas of sensory neurons are located in sensory ganglia (i.e., trigeminal and nodose ganglia). It is well known that within sensory ganglia, sensory neurons do not form neither electrical nor chemical synapses. One of the reasons for this is that each soma is surrounded by glial cells, known as satellite glial cells (SGCs). Recent evidence shows that connexin43 (Cx43) hemichannels and probably pannexons located at SGCs have an important role in paracrine communication between glial cells and sensory neurons...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29198211/association-of-cx43-rs2071166-polymorphism-with-an-increased-risk-for-atrial-septal-defect
#11
Ruoyi Gu, Wei Sheng, Xiaojing Ma, Guoying Huang
Atrial septal defect is one of the most common CHD. The pathogenesis of atrial septal defect still remains unknown. Cx43 is the most prevalent connexin in the mammalian heart during development. Its genetic variants can cause several CHD. The aim of our study was to investigate the association of genetic variations of the Cx43 with sporadic atrial septal defect. A total of 450 paediatric patients were recruited, including 150 cases with atrial septal defect and 300 healthy controls. The promoter region of Cx43 was analysed by sequencing after polymerase chain reaction...
December 4, 2017: Cardiology in the Young
https://www.readbyqxmd.com/read/29197877/functional-characterization-of-a-novel-truncating-mutation-in-lamin-a-c-gene-in-a-family-with-a-severe-cardiomyopathy-with-conduction-defects
#12
Andrea Gerbino, Irene Bottillo, Serena Milano, Martina Lipari, Roberta De Zio, Silvia Morlino, Maria Grazia Mola, Giuseppe Procino, Federica Re, Elisabetta Zachara, Paola Grammatico, Maria Svelto, Monica Carmosino
BACKGROUND/AIMS: Truncating LMNA gene mutations occur in many inherited cardiomyopathy cases, but the molecular mechanisms involved in the disease they cause have not yet been systematically investigated. Here, we studied a novel frameshift LMNA variant (p.D243Gfs*4) identified in three members of an Italian family co-segregating with a severe form of cardiomyopathy with conduction defects. METHODS: HEK293 cells and HL-1 cardiomyocytes were transiently transfected with either Lamin A or D243Gfs*4 tagged with GFP (or mCherry)...
December 4, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29186829/inhibition-by-commercial-aminoglycosides-of-human-connexin-hemichannels-expressed-in-bacteria
#13
Mariana C Fiori, Srinivasan Krishnan, Abbey Kjellgren, Luis G Cuello, Guillermo A Altenberg
In addition to gap junctional channels that mediate cell-to-cell communication, connexins form hemichannels that are present at the plasma membrane. Since hemichannels are permeable to small hydrophilic compounds, including metabolites and signaling molecules, their abnormal opening can cause or contribute to cell damage in disorders such as cardiac infarct, stroke, deafness, skin diseases, and cataracts. Therefore, hemichannels are potential pharmacological targets. A few aminoglycosides, well-known broad-spectrum antibiotics, have been shown to inhibit hemichannels...
November 25, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29185069/connexin-43-and-atp-sensitive-potassium-channels-crosstalk-a-missing-link-in-hypoxia-ischemia-stress
#14
REVIEW
Ajaz Ahmad Waza, Shabir Ahmad Bhat, Mahboob Ul Hussain, Bashir A Ganai
Connexin 43 (Cx43) is a gap junction protein expressed in various tissues and organs of vertebrates. Besides functioning as a gap junction, Cx43 also regulates diverse cellular processes like cell growth and differentiation, cell migration, cell survival, etc. Cx43 is critical for normal cardiac functioning and is therefore abundantly expressed in cardiomyocytes. On the other hand, ATP-sensitive potassium (KATP) channels are metabolic sensors converting metabolic changes into electrical activity. These channels are important in maintaining the neurotransmitter release, smooth muscle relaxation, cardiac action potential repolarization, normal physiology of cellular repolarization, insulin secretion and immune function...
November 29, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/29183963/distinct-moieties-underlie-biphasic-h-gating-of-connexin43-channels-producing-a-ph-optimum-for-intercellular-communication
#15
Carolina D Garciarena, Akif Malik, Pawel Swietach, Alonso P Moreno, Richard D Vaughan-Jones
Most mammalian cells can intercommunicate via connexin-assembled, gap-junctional channels. To regulate signal transmission, connexin (Cx) channel permeability must respond dynamically to physiological and pathophysiological stimuli. One key stimulus is intracellular pH (pHi), which is modulated by a tissue's metabolic and perfusion status. Our understanding of the molecular mechanism of H+ gating of Cx43 channels-the major isoform in the heart and brain-is incomplete. To interrogate the effects of acidic and alkaline pHi on Cx43 channels, we combined voltage-clamp electrophysiology with pHi imaging and photolytic H+ uncaging, performed over a range of pHi values...
November 28, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29181063/impact-of-connexin-43-coupling-on-survival-and-migration-of-multiple-myeloma-cells
#16
Yangmin Zhang, Ziyan Wang, Liying Zhang, Dongming Zhou, Yu Sun, Panjun Wang, Songguang Ju, Ping Chen, Jun Li, Jinxiang Fu
Introduction: Gap junctions (GJs) represent the best known intercellular communication (IC) system and are membrane-spanning channels that facilitate intercellular communication by allowing small signaling molecules to pass from cell to cell. In this study, we constructed an amino terminus of human Cx43 (Cx43NT-GFP), verified the overexpression of Cx43-NT in HUVEC cells and explored the impact of gap junctions (GJs) on multiple myeloma (MM). Material and methods: The levels of phosphorylated Cx43(s368) and the change of MAPK pathway associated molecules (ERK1/2, JNK, p38, NFκB) were also investigated in our cell models...
October 2017: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/29180066/inhibition-of-estrogen-receptor-reduces-connexin-43-expression-in-breast-cancers
#17
Cheng-Fang Tsai, Yu-Kai Cheng, Dah-Yuu Lu, Shu-Lin Wang, Chen-Ni Chang, Pei-Chun Chang, Wei-Lan Yeh
Connexins are widely supported as tumor suppressors due to their downregulation in cancers, nevertheless, more recent evidence suggests roles for connexins in facilitating tumor progression in later stages, including metastasis. One of the key factors regulating the expression, modification, stability, and localization of connexins is hormone receptors in hormone-dependent cancers. It is reasonable to consider that hormones/hormone receptors may modulate connexins expression and play critical roles in the cellular control of connexins during breast cancer progression...
November 24, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29179175/novel-functional-role-of-heat-shock-protein-90-in-mitochondrial-connexin-43-mediated-hypoxic-postconditioning
#18
Rong-Hui Tu, Qing-Jie Li, Zheng Huang, Yan He, Jian-Jun Meng, Hui-Lei Zheng, Zhi-Yu Zeng, Guo-Qiang Zhong
BACKGROUND/AIMS: Previous studies have shown that heat shock protein 90 (HSP90)-mediated mitochondrial import of connexin 43 (Cx43) is critical in preconditioning cardioprotection. The present study was designed to test whether postconditioning has the same effect as preconditioning in promoting Cx43 translocation to mitochondria and whether mitochondrial HSP90 modulates this effect. METHODS: Cellular models of hypoxic postconditioning (HPC) from rat heart-derived H9c2 cells and neonatal rat cardiomyocytes were employed...
November 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29176328/cardiomyopathy-associated-gene-1-sensitive-pkc-dependent-connexin-43-expression-and-phosphorylation-in-left-ventricular-noncompaction-cardiomyopathy
#19
Yuanyuan Xie, Shenghua Liu, Shengshou Hu, Yingjie Wei
BACKGROUND/AIMS: Cardiomyopathy-associated gene 1 (CMYA1) plays an important role in embryonic cardiac development, postnatal cardiac remodeling and myocardial injury repair. Abnormal CMYA1 expression may be involved in cardiac dysplasia and primary cardiomyopathy. Our study aims to establish the relationship between CMYA1 and Left ventricular noncompaction cardiomyopathy (LVNC) pathogenesis. METHODS: We explored the effects of CMYA1 on connexins (Cx), which contribute to gap junction intercellular communication (GJIC), and the underlying signaling pathway in human normal tissues, LVNC myocardial tissues and HL1 cells by means of western blotting, RT-qPCR, immunohistochemistry, immunofluorescence, co-immunoprecipitation and scrape loading-dye transfer...
November 24, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29174980/pdgf-mediated-pi3k-akt-%C3%AE-catenin-signaling-regulates-gap-junctions-in-corpus-cavernosum-smooth-muscle-cells
#20
Xiang Zhang, Fan Zhao, Jian-Feng Zhao, Hui-Ying Fu, Xiao-Jun Huang, Bo-Dong Lv
Erectile dysfunction (ED) is the most common sexual disorder that men report to healthcare providers. Gap junctions (GJs) are thought to be responsible for synchronous shrinkage of corpus cavernosum smooth muscle cells (CCSMCs), and play thus an important role in the maintenance of an erection. Hypoxia has been suggested as a pathological mechanism underlying ED. Here we demonstrate that hypoxia increased the expression of platelet-derived growth factor (PDGF) and the main GJ component connexin (Cx)43 in CCSMCs...
November 22, 2017: Experimental Cell Research
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