keyword
MENU ▼
Read by QxMD icon Read
search

(ROCK OR Rho) AND mitochondri*

keyword
https://www.readbyqxmd.com/read/29773647/altered-sterol-metabolism-in-budding-yeast-affects-mitochondrial-iron-sulfur-fe-s-cluster-synthesis
#1
Diane M Ward, Opal S Chen, Liangtao Li, Jerry Kaplan, Shah Alam Bhuiyan, Selvamuthu K Natarajan, Martin Bard, James E Cox
Ergosterol synthesis is essential for cellular growth and viability of the budding yeast Saccharomyces cerevisiae, and intracellular sterol distribution and homeostasis are therefore highly regulated in this species. Erg25 is an iron-containing C4-methyl sterol oxidase that contributes to the conversion of 4,4-dimethylzymosterol to zymosterol, a precursor of ergosterol. The ERG29 gene encodes an endoplasmic reticulum (ER)-associated protein, and here we identified a role for Erg29 in the methyl sterol oxidase step of ergosterol synthesis...
May 17, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29720839/protective-effect-of-water-extracted-spirulina-maxima-on-glutamate-induced-neuronal-cell-death-in-mouse-hippocampal-ht22-cell
#2
Hyeon Yong Lee, Ga Hee Ryu, Woon Yong Choi, Woo Seung Yang, Hyeon Woo Lee, Choong Je Ma
Introduction: Spirulina maxima was used as important nutritional source in the Aztec civilization because it is rich in proteins and vitamins. It contains various antioxidants such as phycocyanin and flavonoids. Based on abundant antioxidants, S. maxima is known to possess anti-inflammatory effect, especially on neuronal cells. Materials and Methods: S. maxima was extracted in water and contain of phycocyanin was identified by high-performance liquid chromatography...
April 2018: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29702701/glycolysis-regulates-pollen-tube-polarity-via-rho-gtpase-signaling
#3
Wei Chen, Pingping Gong, Jingzhe Guo, Hui Li, Ruizi Li, Weiman Xing, Zhenbiao Yang, Yuefeng Guan
As a universal energy generation pathway utilizing carbon metabolism, glycolysis plays an important housekeeping role in all organisms. Pollen tubes expand rapidly via a mechanism of polarized growth, known as tip growth, to deliver sperm for fertilization. Here, we report a novel and surprising role of glycolysis in the regulation of growth polarity in Arabidopsis pollen tubes via impingement of Rho GTPase-dependent signaling. We identified a cytosolic phosphoglycerate kinase (pgkc-1) mutant with accelerated pollen germination and compromised pollen tube growth polarity...
April 27, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29698604/cytocompatibility-and-cellular-internalization-of-pegylated-clickable-nucleic-acid-oligomers
#4
Alex J Anderson, Erica B Peters, Alex Neumann, Justine Wagner, Benjamin Fairbanks, Stephanie J Bryant, Christopher N Bowman
The recently developed synthetic oligonucleotides referred to as "Click" Nucleic Acids (CNAs) are promising due to their relatively simple synthesis based on thiol-X reactions with numerous potential applications in biotechnology, biodetection, gene silencing, and drug delivery. Here, the cytocompatibility and cellular uptake of rhodamine tagged, PEGylated CNA copolymers (PEG-CNA-RHO) were evaluated. NIH 3T3 fibroblast cells treated for 1 hour with 1, 10 or 100 μg/mL PEG-CNA-RHO maintained an average cell viability of 86%, which was not significantly different from the untreated control...
April 26, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29686202/a-new-species-of-gonionemus-hydrozoa-limnomedusae-from-southern-australia
#5
Jeanette E Watson, Annette F Govindarajan
A new species of a crawling limnomedusa belonging to the genus Gonionemus (Hydrozoa: Limnomedusae: Olindiidae) was collected from the brown alga Cystophora monilifera in an intertidal rock pool in Western Port, Victoria, Australia. The new species is described and compared with the three known species of Gonionemus. The mitochondrial DNA barcode markers cytochrome oxidase I and 16S rRNA were sequenced and compared to sequences from other olindiid species. The sequencing results corroborate the morphological findings...
December 19, 2017: Zootaxa
https://www.readbyqxmd.com/read/29684861/inhibition-of-gastric-cancer-by-local-anesthetic-bupivacaine-through-multiple-mechanisms-independent-of-sodium-channel-blockade
#6
Jiapeng Dan, Xiaodan Gong, Dezhan Li, Guanhua Zhu, Lishen Wang, Fang Li
BACKGROUND: Retrospective studies of patients undergoing cancer surgery suggest the use of local anesthesia may decrease tumor recurrence and improve survival. The mechanisms on the benefits of local anesthesia on cancer recurrence are complex and remain to be elucidated. METHODS: This study investigated the effects of bupivacaine on various cellular activities of gastric cancer using proliferation, migration, apoptosis assay. The underlying mechanism was analyzed focusing on mitochondrial functions and the activities of Rho family members...
April 20, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29626418/bergamot-natural-products-eradicate-cancer-stem-cells-cscs-by-targeting-mevalonate-rho-gdi-signalling-and-mitochondrial-metabolism
#7
Marco Fiorillo, Maria Peiris-Pagès, Rosa Sanchez-Alvarez, Lucia Bartella, Leonardo Di Donna, Vincenza Dolce, Giovanni Sindona, Federica Sotgia, Anna Rita Cappello, Michael P Lisanti
Here, we show that a 2:1 mixture of Brutieridin and Melitidin, termed "BMF", has a statin-like properties, which blocks the action of the rate-limiting enzyme for mevalonate biosynthesis, namely HMGR (3-hydroxy-3-methylglutaryl-CoA-reductase). Moreover, our results indicate that BMF functionally inhibits several key characteristics of CSCs. More specifically, BMF effectively i) reduced ALDH activity, ii) blocked mammosphere formation and iii) inhibited the activation of CSC-associated signalling pathways (STAT1/3, Notch and Wnt/beta-catenin) targeting Rho-GDI-signalling...
April 4, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29511132/a-first-in-human-phase-1-study-of-opb-111077-a-small-molecule-stat3-and-oxidative-phosphorylation-inhibitor-in-patients-with-advanced-cancers
#8
Anthony Tolcher, Keith Flaherty, Geoffrey I Shapiro, Jordan Berlin, Thomas Witzig, Thomas Habermann, Andrea Bullock, Edwin Rock, Agnes Elekes, Chester Lin, Dusan Kostic, Naoto Ohi, Drew Rasco, Kyriakos P Papadopoulos, Amita Patnaik, Lon Smith, Gregory M Cote
LESSONS LEARNED: OPB-111077 is a novel inhibitor of STAT3 and mitochondrial oxidative phosphorylation that exhibited promising anticancer activity in preclinical models.In this first-in-human phase I study of OPB-111077 in unselected advanced cancers, treatment-emergent adverse events, most frequently nausea, fatigue, and vomiting, were generally mild to moderate in intensity and could be medically managed.Overall, only modest clinical activity was observed after OPB-111077 given as monotherapy...
March 6, 2018: Oncologist
https://www.readbyqxmd.com/read/29491429/simulated-microgravity-inhibits-cell-focal-adhesions-leading-to-reduced-melanoma-cell-proliferation-and-metastasis-via-fak-rhoa-regulated-mtorc1-and-ampk-pathways
#9
Xin Tan, Aizhang Xu, Tuo Zhao, Qin Zhao, Jun Zhang, Cuihong Fan, Yulin Deng, Andrew Freywald, Harald Genth, Jim Xiang
Simulated microgravity (SMG) was reported to affect tumor cell proliferation and metastasis. However, the underlying mechanism is elusive. In this study, we demonstrate that clinostat-modelled SMG reduces BL6-10 melanoma cell proliferation, adhesion and invasiveness in vitro and decreases tumor lung metastasis in vivo. It down-regulates metastasis-related integrin α6β4, MMP9 and Met72 molecules. SMG significantly reduces formation of focal adhesions and activation of focal adhesion kinase (FAK) and Rho family proteins (RhoA, Rac1 and Cdc42) and of mTORC1 kinase, but activates AMPK and ULK1 kinases...
February 28, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29485634/melatonin-modulates-neuronal-mitochondria-function-during-normal-ageing-in-mice
#10
A J Idowu, S L Kumar, B Yidong, R Russel
Mitochondrial dysfunction has been shown to be associated with normal ageing and may account for age-related vulnerability to disease. The increasing number of old people worldwide has created the need to find effective therapeutic agents to reduce the incidence of age-related disease. In the current report, we carried out an assessment of mitochondrial function in established young, middle-aged and old synaptosomal mitochondria bearing cybrids without or with melatonin treatment. The cybrids were generated by transferring isolated mitochondria from synaptosomes of brain cortical cells in mice to rho-zero mtDNA-less cells...
December 30, 2017: Nigerian Journal of Physiological Sciences: Official Publication of the Physiological Society of Nigeria
https://www.readbyqxmd.com/read/29481548/dna-memochip-long-term-and-high-capacity-information-storage-and-select-retrieval
#11
George B Stefano, Fuzhou Wang, Richard M Kream
Over the course of history, human beings have never stopped seeking effective methods for information storage. From rocks to paper, and through the past several decades of using computer disks, USB sticks, and on to the thin silicon "chips" and "cloud" storage of today, it would seem that we have reached an era of efficiency for managing innumerable and ever-expanding data. Astonishingly, when tracing this technological path, one realizes that our ancient methods of informational storage far outlast paper (10,000 vs...
February 26, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29474864/a-crucial-role-of-rock-for-alleviation-of-senescence-associated-phenotype
#12
Joon Tae Park, Hyun Tae Kang, Chi Hyun Park, Young-Sam Lee, Kyung A Cho, Sang Chul Park
In our previous study, we uncovered a novel mechanism in which amelioration of Hutchinson-Gilford progeria syndrome (HGPS) phenotype is mediated by mitochondrial functional recovery upon rho-associated protein kinase (ROCK) inhibition. However, it remains elusive whether this mechanism is also applied to the amelioration of normal aging cells. In this study, we used Y-27632 and fasudil as effective ROCK inhibitors, and examined their role in senescence. We found that ROCK inhibition induced the functional recovery of the mitochondria as well as the metabolic reprogramming, which are two salient features that are altered in normal aging cells...
June 2018: Experimental Gerontology
https://www.readbyqxmd.com/read/29472719/epigenetic-heterogeneity-affects-the-risk-of-relapse-in-children-with-t-8-21-runx1-runx1t1-rearranged-aml
#13
Matteo Zampini, Claudia Tregnago, Valeria Bisio, Luca Simula, Giulia Borella, Elena Manara, Carlo Zanon, Francesca Zonta, Valentina Serafin, Benedetta Accordi, Silvia Campello, Barbara Buldini, Andrea Pession, Franco Locatelli, Giuseppe Basso, Martina Pigazzi
The somatic translocation t(8;21)(q22;q22)/RUNX1-RUNX1T1 is one of the most frequent rearrangements found in children with standard-risk acute myeloid leukemia (AML). Despite the favorable prognostic role of this aberration, we recently observed a higher than expected frequency of relapse. Here, we employed an integrated high-throughput approach aimed at identifying new biological features predicting relapse among 34 t(8;21)-rearranged patients. We found that the DNA methylation status of patients who suffered from relapse was peculiarly different from that of children maintaining complete remission...
May 2018: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
https://www.readbyqxmd.com/read/29452094/evolving-mechanisms-of-vascular-smooth-muscle-contraction-highlight-key-targets-in-vascular-disease
#14
REVIEW
Zhongwei Liu, Raouf A Khalil
Vascular smooth muscle (VSM) plays an important role in the regulation of vascular function. Identifying the mechanisms of VSM contraction has been a major research goal in order to determine the causes of vascular dysfunction and exaggerated vasoconstriction in vascular disease. Major discoveries over several decades have helped to better understand the mechanisms of VSM contraction. Ca2+ has been established as a major regulator of VSM contraction, and its sources, cytosolic levels, homeostatic mechanisms and subcellular distribution have been defined...
February 13, 2018: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29435058/ursolic-acid-activates-the-apoptosis-of-prostate-cancer-via-rock-pten-mediated-mitochondrial-translocation-of-cofilin-1
#15
Dawei Mu, Gaobiao Zhou, Jianye Li, Bin Su, Heqing Guo
Ursolic acid has various pharmacological activities, and can reduce blood fat as well as having antihepatic, antitumoral, anti-inflammatory and antiviral properties. However, the pro-apoptotic mechanism by which ursolic acid influences human prostate cancer requires additional study. The aim of the present study was to assess whether ursolic acid activates the apoptosis of prostate cancer and to investigate the mechanism by which the Rho-associated protein kinase 1 (ROCK1)/phosphatase and tensin homolog (PTEN) signaling pathway performs a role in ursolic acid-mediated cofilin-1 to induce apoptosis in human prostate cancer...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29428217/oxidative-stress-caspase-3-activation-and-cleavage-of-rock-1-play-an-essential-role-in-mehg-induced-cell-death-in-primary-astroglial-cells
#16
Alessandra Antunes Dos Santos, Caridad López-Granero, Marcelo Farina, João B T Rocha, Aaron B Bowman, Michael Aschner
Methylmercury is a toxic environmental contaminant that elicits significant toxicity in humans. The central nervous system is the primary target of toxicity, and is particularly vulnerable during development. Rho-associated protein kinase 1 (ROCK-1) is a major downstream effector of the small GTPase RhoA and a direct substrate of caspase-3. The activation of ROCK-1 is necessary for membrane blebbing during apoptosis. In this work, we examined whether MeHg could affect the RhoA/ROCK-1 signaling pathway in primary cultures of mouse astrocytes...
March 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29421168/associations-of-urinary-phthalate-metabolites-and-lipid-peroxidation-with-sperm-mitochondrial-dna-copy-number-and-deletions
#17
Alexandra M Huffman, Haotian Wu, Allyson Rosati, Tayyab Rahil, Cynthia K Sites, Brian W Whitcomb, J Richard Pilsner
BACKGROUND: Phthalates, a chemical class of plasticizers, are ubiquitous environmental contaminants that have been associated with oxidative stress. Mitochondria DNA copy number (mtDNAcn) and DNA deletions (mtDNAdel) are emerging biomarkers for cellular oxidative stress and environment exposures. OBJECTIVES: To examine associations of urinary phthalate metabolite and isoprostane concentrations on sperm mtDNAcn and mtDNAdel in male partners undergoing assisted reproductive technologies (ART)...
May 2018: Environmental Research
https://www.readbyqxmd.com/read/29396473/decreased-tesk1-mediated-cofilin-1-phosphorylation-in-the-jejunum-of-ibs-d-patients-may-explain-increased-female-predisposition-to-epithelial-dysfunction
#18
Bruno K Rodiño-Janeiro, Cristina Martínez, Marina Fortea, Beatriz Lobo, Marc Pigrau, Adoración Nieto, Ana María González-Castro, Eloísa Salvo-Romero, Danila Guagnozzi, Cristina Pardo-Camacho, Cristina Iribarren, Fernando Azpiroz, Carmen Alonso-Cotoner, Javier Santos, Maria Vicario
Disturbed intestinal epithelial barrier and mucosal micro-inflammation characterize irritable bowel syndrome (IBS). Despite intensive research demonstrating ovarian hormones modulation of IBS severity, there is still limited knowledge on the mechanisms underlying female predominance in this disorder. Our aim was to identify molecular pathways involved in epithelial barrier dysfunction and female predominance in diarrhea-predominant IBS (IBS-D) patients. Total RNA and protein were obtained from jejunal mucosal biopsies from healthy controls and IBS-D patients meeting the Rome III criteria...
February 2, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29373639/a-novel-metabolism-based-phenotypic-drug-discovery-platform-in-zebrafish-uncovers-hdacs-1-and-3-as-a-potential-combined-anti-seizure-drug-target
#19
Kingsley Ibhazehiebo, Cezar Gavrilovici, Cristiane L de la Hoz, Shun-Chieh Ma, Renata Rehak, Gaurav Kaushik, Paola L Meza Santoscoy, Lucas Scott, Nandan Nath, Do-Young Kim, Jong M Rho, Deborah M Kurrasch
Despite the development of newer anti-seizure medications over the past 50 years, 30-40% of patients with epilepsy remain refractory to treatment. One explanation for this lack of progress is that the current screening process is largely biased towards transmembrane channels and receptors, and ignores intracellular proteins and enzymes that might serve as efficacious molecular targets. Here, we report the development of a novel drug screening platform that harnesses the power of zebrafish genetics and combines it with in vivo bioenergetics screening assays to uncover therapeutic agents that improve mitochondrial health in diseased animals...
January 24, 2018: Brain: a Journal of Neurology
https://www.readbyqxmd.com/read/29365285/ectopic-expression-of-miro-1-ameliorates-seizure-and-inhibits-hippocampal-neurodegeneration-in-a-mouse-pilocarpine-epilepsy-model
#20
Haifeng Zhang, Yajun Lian, Nanchang Xie, Yake Zheng
Epilepsy is a common disease of the central nervous system. This study aims to investigate the role of mitochondrial Rho (Miro) 1 in epilepsy using a mouse model of pilocarpine-induced status epilepticus (SE). Intraperitoneal injection of pilocarpine induced epileptic seizure in mice and significantly decreased Miro 1 expression in the hippocampus. Moreover, pilocarpine treatment increased the serum levels of heat shock protein 70 (HSP70) and S100 calcium binding protein B (S100B), and led to hippocampal neuronal injury and apoptosis...
January 24, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
keyword
keyword
85740
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"